Starting /dee2/code/volunteer_pipeline.sh ERR4018152
    current disk space = 357305448
    free memory = 198542664 
ERR4018152 SRAfilesize
34d8db9692c02b4e8ed7f57366103aa9  ERR4018152.sra
ERR4018152.sra file validated
ERR4018152 is single end
ERR4018152 is conventional basespace
ERR4018152 read1 length is 76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR4018152_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	76
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.345	32.0	32.0	32.0	32.0	32.0
2	31.39575	32.0	32.0	32.0	32.0	32.0
3	31.587	32.0	32.0	32.0	32.0	32.0
4	31.645	32.0	32.0	32.0	32.0	32.0
5	31.626	32.0	32.0	32.0	32.0	32.0
6	34.7775	36.0	36.0	36.0	36.0	36.0
7	35.263	36.0	36.0	36.0	36.0	36.0
8	35.1995	36.0	36.0	36.0	36.0	36.0
9	35.21325	36.0	36.0	36.0	36.0	36.0
10-11	35.23675	36.0	36.0	36.0	36.0	36.0
12-13	35.148250000000004	36.0	36.0	36.0	36.0	36.0
14-15	35.21425	36.0	36.0	36.0	36.0	36.0
16-17	35.213625	36.0	36.0	36.0	36.0	36.0
18-19	35.17375	36.0	36.0	36.0	36.0	36.0
20-21	35.212	36.0	36.0	36.0	36.0	36.0
22-23	35.1225	36.0	36.0	36.0	36.0	36.0
24-25	35.051500000000004	36.0	36.0	36.0	36.0	36.0
26-27	34.9265	36.0	36.0	36.0	34.0	36.0
28-29	34.822500000000005	36.0	36.0	36.0	32.0	36.0
30-31	34.973375000000004	36.0	36.0	36.0	36.0	36.0
32-33	34.981625	36.0	36.0	36.0	36.0	36.0
34-35	35.1005	36.0	36.0	36.0	36.0	36.0
36-37	35.01625	36.0	36.0	36.0	36.0	36.0
38-39	35.063874999999996	36.0	36.0	36.0	36.0	36.0
40-41	34.84525	36.0	36.0	36.0	34.0	36.0
42-43	34.9055	36.0	36.0	36.0	36.0	36.0
44-45	35.05825	36.0	36.0	36.0	36.0	36.0
46-47	35.002250000000004	36.0	36.0	36.0	36.0	36.0
48-49	34.7975	36.0	36.0	36.0	34.0	36.0
50-51	34.73875	36.0	36.0	36.0	34.0	36.0
52-53	34.48775	36.0	36.0	36.0	32.0	36.0
54-55	34.344125000000005	36.0	36.0	36.0	32.0	36.0
56-57	33.797	36.0	36.0	36.0	29.5	36.0
58-59	33.285624999999996	36.0	34.0	36.0	24.0	36.0
60-61	33.404875000000004	36.0	36.0	36.0	27.0	36.0
62-63	32.870375	36.0	32.0	36.0	21.0	36.0
64-65	32.935625	36.0	32.0	36.0	24.0	36.0
66-67	33.46325	36.0	36.0	36.0	27.0	36.0
68-69	33.617999999999995	36.0	36.0	36.0	27.0	36.0
70-71	33.302499999999995	36.0	36.0	36.0	24.0	36.0
72-73	33.146375	36.0	36.0	36.0	24.0	36.0
74-75	33.48725	36.0	36.0	36.0	27.0	36.0
76	31.57425	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	1.0
22	3.0
23	6.0
24	11.0
25	22.0
26	34.0
27	43.0
28	56.0
29	74.0
30	100.0
31	122.0
32	186.0
33	306.0
34	743.0
35	2292.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.175000000000004	18.475	13.4	24.95
2	35.125	33.825	9.725	21.325
3	15.299999999999999	28.000000000000004	9.85	46.85
4	31.3	9.725	42.15	16.825000000000003
5	17.974999999999998	26.275	27.1	28.65
6	24.892812105926858	9.634300126103405	35.40983606557377	30.063051702395967
7	28.749999999999996	13.525	33.800000000000004	23.925
8	16.875	27.150000000000002	48.4	7.575
9	44.35	13.0	16.0	26.650000000000002
10-11	44.625	17.2625	18.8125	19.3
12-13	27.0875	21.837500000000002	33.4875	17.5875
14-15	43.925	24.7875	15.45	15.837499999999999
16-17	30.55	29.575000000000003	32.1125	7.7625
18-19	39.300000000000004	14.725	27.150000000000002	18.825
20-21	34.949999999999996	21.7	31.324999999999996	12.025
22-23	25.6	30.325000000000003	27.6	16.475
24-25	30.175	17.7375	35.65	16.4375
26-27	17.483741870935468	18.396698349174585	33.379189594797396	30.740370185092548
28-29	35.391358797745774	11.659361302442079	28.040075140889165	24.909204758922982
30-31	28.921691268451337	7.443082311733801	29.334500875656744	34.30072554415812
32-33	43.425000000000004	9.8375	25.224999999999998	21.512500000000003
34-35	40.9625	22.5625	15.6125	20.8625
36-37	30.425	29.25	23.5875	16.7375
38-39	25.324999999999996	25.074999999999996	19.35	30.25
40-41	21.775	31.4625	16.6875	30.075000000000003
42-43	40.025	23.6375	14.2625	22.075
44-45	26.787499999999998	28.175	17.9375	27.1
46-47	25.587500000000002	21.25	18.5	34.6625
48-49	15.4	30.9625	14.674999999999999	38.9625
50-51	30.1375	30.55	12.7875	26.525
52-53	28.512500000000003	31.7375	19.287499999999998	20.4625
54-55	18.1625	40.0875	14.887500000000001	26.8625
56-57	18.212500000000002	33.050000000000004	23.5375	25.2
58-59	17.2	36.075	20.5	26.224999999999998
60-61	17.1875	33.375	27.6125	21.825
62-63	16.7125	29.9875	27.787499999999998	25.5125
64-65	19.9375	19.1375	31.15	29.775000000000002
66-67	29.6625	16.162499999999998	29.8875	24.2875
68-69	21.675	31.1	25.687500000000004	21.5375
70-71	18.862499999999997	27.575	29.3375	24.224999999999998
72-73	15.975	11.7875	38.2	34.0375
74-75	15.5	8.512500000000001	40.4125	35.575
76	28.749999999999996	7.625	36.85	26.775
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.5
23	0.5
24	0.0
25	0.0
26	0.0
27	1.0
28	1.5
29	1.0
30	1.5
31	1.5
32	1.0
33	1.0
34	3.5
35	7.5
36	6.0
37	3.0
38	2.5
39	3.0
40	4.5
41	8.5
42	12.0
43	20.0
44	34.0
45	85.0
46	130.0
47	196.5
48	326.0
49	417.5
50	446.0
51	724.5
52	769.0
53	446.0
54	357.0
55	323.0
56	216.5
57	191.5
58	239.0
59	152.0
60	41.0
61	10.0
62	3.0
63	1.5
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.8750000000000001
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.05
28-29	0.1875
30-31	0.075
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.04522246535376	26.75
2	8.533916849015318	5.8500000000000005
3	4.230488694383661	4.35
4	1.604668125455872	2.1999999999999997
5	1.0940919037199124	1.875
6	0.802334062727936	1.6500000000000001
7	0.7293946024799417	1.7500000000000002
8	0.802334062727936	2.1999999999999997
9	0.36469730123997085	1.125
>10	2.9905178701677606	19.625
>50	0.437636761487965	9.85
>100	0.36469730123997085	22.775000000000002
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GACTCTTAGCGGTGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	336	8.4	RNA PCR Primer, Index 1 (100% over 33bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	241	6.0249999999999995	No Hit
CGCGACCTCAGATCAGACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	118	2.9499999999999997	RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA	112	2.8000000000000003	No Hit
ACAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	104	2.6	RNA PCR Primer, Index 1 (100% over 29bp)
GACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	79	1.975	RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	76	1.9	RNA PCR Primer, Index 1 (100% over 30bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA	63	1.575	RNA PCR Primer, Index 1 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	61	1.525	No Hit
GACTCTTAGCGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG	61	1.525	RNA PCR Primer, Index 15 (97% over 36bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCTGGAATTCTCGGGTGC	54	1.35	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	49	1.225	No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGTGGAATTCTCGGGTGCCAAG	42	1.05	No Hit
CGCGACCTCAGATCAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	37	0.9249999999999999	RNA PCR Primer, Index 1 (100% over 32bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCTGGAATTCTCGGGTG	30	0.75	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAG	29	0.7250000000000001	No Hit
CGCGACCTCAGATCAGACGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	27	0.675	RNA PCR Primer, Index 1 (100% over 29bp)
CAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	24	0.6	RNA PCR Primer, Index 1 (100% over 30bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	24	0.6	No Hit
AGCTACATTGTCTGCTGGGTTTCTGGAATTCTCGGGTGCCAAGGAACTCC	24	0.6	RNA PCR Primer, Index 1 (100% over 27bp)
GACTCTTAGCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT	23	0.575	RNA PCR Primer, Index 1 (97% over 35bp)
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG	21	0.525	No Hit
TGGCTCAGTTCAGCAGGAACAGTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
GGCCGCCGGTGAAATACCACTACTCTGGAATTCTCGGGTGCCAAGGAACT	21	0.525	RNA PCR Primer, Index 1 (100% over 25bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC	21	0.525	RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	21	0.525	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA	19	0.475	RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA	18	0.44999999999999996	No Hit
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCT	17	0.42500000000000004	RNA PCR Primer, Index 19 (100% over 43bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC	17	0.42500000000000004	No Hit
GTTTCCGTAGTGTAGTGGTTATCTGGAATTCTCGGGTGCCAAGGAACTCC	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 27bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	17	0.42500000000000004	No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCTGGAATTCTCGGGTGCCAA	16	0.4	No Hit
ATCCCGGGGCCGAGGGAGCCGGATTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG	14	0.35000000000000003	No Hit
GACTCTTAGCGGTGGATCACTTGGAATTCTCGGGTGCCAAGGAACTCCAG	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCATTGCTGTCGGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG	14	0.35000000000000003	RNA PCR Primer, Index 15 (97% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGTGGAATTCTCGGGTGCCA	14	0.35000000000000003	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	13	0.325	No Hit
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGTGGAATTCTCGGGTGCCAA	13	0.325	No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA	12	0.3	No Hit
GTTTCCGTAGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAA	12	0.3	RNA PCR Primer, Index 19 (100% over 38bp)
ACAGTAGTCTGCACATTGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCTGGAATTCTCGGGTGCCA	10	0.25	No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTTGGAATTCTCGGGTGCCAAGG	10	0.25	Illumina Small RNA Adapter 2 (100% over 21bp)
CGACTCTTAGCGGTGGATCACTCTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
CGACTCTTAGCGGTGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	10	0.25	RNA PCR Primer, Index 1 (100% over 32bp)
GACTCTTAGCGGTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	9	0.22499999999999998	RNA PCR Primer, Index 6 (100% over 34bp)
ACAGTAGTCTGCACATTGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
GGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCAAGG	9	0.22499999999999998	Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 33bp)
TGGTGCGGACCAGGGGAATCCGACTGTTTGGAATTCTCGGGTGCCAAGGA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 22bp)
AATCCCGGGGCCGAGGGAGCCGGATTGGAATTCTCGGGTGCCAAGGAACT	8	0.2	RNA PCR Primer, Index 1 (100% over 25bp)
TCTTAGCGGTGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG	8	0.2	RNA PCR Primer, Index 15 (97% over 36bp)
GACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAAC	8	0.2	RNA PCR Primer, Index 1 (100% over 24bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AAATGGATTTTTGGAACTAGGAGTTGGAATTCTCGGGTGCCAAGGAACTC	8	0.2	RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
TGGAAGACTAGTGATTTTGTTGTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCATGG	8	0.2	No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAAG	8	0.2	No Hit
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
ATGGATTTTTGGAACTAGGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
GAGGTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAGGAACT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 33bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
GGCCGCCGGTGAAATACCACTACTTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
GACTCTTAGCGGTGGATCATGGAATTCTCGGGTGCCAAGGAACTCCAGTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
GGTGCGGACCAGGGGAATCCGACTGTTTGGAATTCTCGGGTGCCAAGGAA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 23bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTGGAATTCTCGGGTGCCA	7	0.17500000000000002	No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA	6	0.15	RNA PCR Primer, Index 1 (100% over 23bp)
GGCCGCCGGTGAAATACCACTACTCTCATGGAATTCTCGGGTGCCAAGGA	6	0.15	RNA PCR Primer, Index 1 (100% over 22bp)
CGACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	6	0.15	No Hit
AATGGATTTTTGGAACTAGGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT	6	0.15	RNA PCR Primer, Index 1 (97% over 35bp)
ATCCCGGGGCCGAGGGAGCCGGATGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GGTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AATCCCGGGGCCGAGGGAGCCGGATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
GACTCTTAGCGGTGGATTGGAATTCTCGGGTGCCAGGAACTCCAGTCACG	5	0.125	No Hit
AACATTCATTGCTGTCGGTGGGTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GGTGCGGACCAGGGGAATCCGACTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ATACATGATGATCTCACTCCAACTTGAACTCTCTCACTGATTACTTGATG	5	0.125	No Hit
ACGACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
AGGCAGTGTAGTTAGCTGATTGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	5	0.125	RNA PCR Primer, Index 6 (100% over 34bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCATG	5	0.125	No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG	5	0.125	RNA PCR Primer, Index 15 (97% over 36bp)
AAAGGTTTGGTCCTGGCCTTATAATTAATTGGAGGTAAGATTATGGAATT	5	0.125	No Hit
CGACTCTTAGCGGTGGATCACTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GACTCTTAGCGGTGGATCACTCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
ATGGATTTTTGGAACTAGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.05	0.0	0.0	0.0
7	0.0	0.225	0.0	0.0	0.0
8	0.0	0.9	0.0	0.0	0.0
9	0.0	1.225	0.0	0.0	0.0
10	0.0	1.8	0.0	0.0	0.0
11	0.0	2.35	0.0	0.0	0.0
12	0.0	2.875	0.0	0.0	0.0
13	0.0	3.675	0.0	0.0	0.0
14	0.0	4.5	0.0	0.0	0.0
15	0.0	7.2	0.0	0.0	0.0
16	0.0	8.675	0.0	0.0	0.0
17	0.0	10.375	0.0	0.0	0.0
18	0.0	21.05	0.0	0.0	0.0
19	0.0	25.4	0.0	0.0	0.0
20	0.0	30.1	0.0	0.0	0.0
21	0.0	34.4	0.0	0.0	0.0
22	0.0	41.4	0.0	0.0	0.0
23	0.0	49.475	0.0	0.0	0.0
24	0.0	55.65	0.0	0.0	0.0
25	0.0	58.6	0.0	0.0	0.0
26	0.0	60.9	0.0	0.0	0.0
27	0.0	62.375	0.0	0.0	0.0
28	0.0	63.725	0.0	0.0	0.0
29	0.0	65.5	0.0	0.0	0.0
30	0.0	66.85	0.0	0.0	0.0
31	0.0	70.875	0.0	0.0	0.0
32	0.0	83.4	0.0	0.0	0.0
33	0.0	86.625	0.0	0.0	0.0
34	0.0	87.95	0.0	0.0	0.0
35	0.0	90.1	0.0	0.0	0.0
36	0.0	91.225	0.0	0.0	0.0
37	0.0	91.425	0.0	0.0	0.0
38	0.0	91.65	0.0	0.0	0.0
39	0.0	91.9	0.0	0.0	0.0
40	0.0	92.1	0.0	0.0	0.0
41	0.0	92.325	0.0	0.0	0.0
42	0.0	92.425	0.0	0.0	0.0
43	0.0	92.675	0.0	0.0	0.0
44	0.0	92.925	0.0	0.0	0.0
45	0.0	93.175	0.0	0.0	0.0
46	0.0	93.25	0.0	0.0	0.0
47	0.0	93.375	0.0	0.0	0.0
48	0.0	93.625	0.0	0.0	0.0
49	0.0	93.775	0.0	0.0	0.0
50	0.0	94.425	0.0	0.0	0.0
51	0.0	94.825	0.0	0.0	0.0
52	0.0	95.05	0.0	0.0	0.0
53	0.0	95.1	0.0	0.0	0.0
54	0.0	95.175	0.0	0.0	0.0
55	0.0	95.2	0.0	0.0	0.0
56	0.0	95.25	0.0	0.0	0.0
57	0.0	95.275	0.0	0.0	0.0
58	0.0	95.375	0.0	0.0	0.0
59	0.0	95.4	0.0	0.0	0.0
60	0.0	95.475	0.0	0.0	0.0
61	0.0	95.475	0.0	0.0	0.0
62	0.0	95.55	0.0	0.0	0.0
63	0.0	95.6	0.0	0.0	0.0
64	0.0	95.625	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGACC	60	0.0	70.00001	1
GAGGTAG	15	0.0020658788	70.00001	2
AGCTTAT	15	0.0020658788	70.00001	2
ACTGATG	15	0.0020658788	70.00001	12
TTGTATA	15	0.0020658788	70.00001	13
GCGACCT	60	0.0	70.00001	2
GCGACCC	30	7.878043E-8	70.00001	22
CCGCTTG	15	0.0020658788	70.00001	27
GATGTTG	15	0.0020658788	70.00001	15
TAGCTTA	15	0.0020658788	70.00001	1
TTATCAG	15	0.0020658788	70.00001	5
CTCTTAG	60	0.0	70.00001	3
ACTCTTA	60	0.0	70.00001	2
GGCGACC	30	7.878043E-8	70.00001	21
AGAATTC	45	3.6379788E-12	70.00001	20
GTATAGT	15	0.0020658788	70.00001	15
CCCGCTT	15	0.0020658788	70.00001	26
CGTGGCG	35	2.6138878E-9	70.00001	18
AGACGTG	45	3.6379788E-12	70.00001	15
TCTCGCC	45	3.6379788E-12	70.00001	25
>>END_MODULE
Rejected 3595350 READS because READLEN < 1
Read 3595350 spots for ERR4018152.sra
Written 3595350 spots for ERR4018152.sra
Rejected 3595350 READS because READLEN < 1
Read 3595350 spots for ERR4018152.sra
Written 3595350 spots for ERR4018152.sra
Rejected 3595350 READS because READLEN < 1
Read 3595350 spots for ERR4018152.sra
Written 3595350 spots for ERR4018152.sra
Rejected 3595350 READS because READLEN < 1
Read 3595350 spots for ERR4018152.sra
Written 3595350 spots for ERR4018152.sra
Rejected 3595350 READS because READLEN < 1
Read 3595350 spots for ERR4018152.sra
Written 3595350 spots for ERR4018152.sra
Rejected 3595350 READS because READLEN < 1
Read 3595350 spots for ERR4018152.sra
Written 3595350 spots for ERR4018152.sra
Rejected 3595352 READS because READLEN < 1
Read 3595352 spots for ERR4018152.sra
Written 3595352 spots for ERR4018152.sra
Rejected 3595350 READS because READLEN < 1
Read 3595350 spots for ERR4018152.sra
Written 3595350 spots for ERR4018152.sra
SRR ids: ['ERR4018152.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_nfjnoo22
ERR4018152.sra spots: 28762802
blocks: [[1, 3595350], [3595351, 7190700], [7190701, 10786050], [10786051, 14381400], [14381401, 17976750], [17976751, 21572100], [21572101, 25167450], [25167451, 28762802]]
ERR4018152 file size 5483684
ERR4018152 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o ERR4018152 ERR4018152_1.fastq
Input file:	ERR4018152_1.fastq
trimmed:	ERR4018152-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Sun Dec 28 15:07:34 2025 >> started

Sun Dec 28 15:07:53 2025 >> done (19.483s)
28762802 reads processed; of these:
       0 ( 0.00%) short reads filtered out after trimming by size control
       0 ( 0.00%) empty reads filtered out after trimming by size control
28762802 (100.00%) reads available; of these:
    5361 ( 0.02%) trimmed reads available after processing
28757441 (99.98%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 42	      99	  0.00%
 43	     132	  0.00%
 44	      92	  0.00%
 45	     630	  0.00%
 46	     709	  0.00%
 47	     740	  0.00%
 48	     580	  0.00%
 49	     747	  0.00%
 50	     195	  0.00%
 51	     298	  0.00%
 52	     525	  0.00%
 53	      26	  0.00%
 54	     207	  0.00%
 55	      27	  0.00%
 56	       9	  0.00%
 57	      13	  0.00%
 58	       4	  0.00%
 59	       0	  0.00%
 60	       0	  0.00%
 61	       3	  0.00%
 62	       0	  0.00%
 63	       7	  0.00%
 64	       0	  0.00%
 65	       0	  0.00%
 66	       0	  0.00%
 67	      34	  0.00%
 68	      12	  0.00%
 69	     110	  0.00%
 70	      10	  0.00%
 71	       6	  0.00%
 72	       5	  0.00%
 73	      58	  0.00%
 74	      18	  0.00%
 75	      65	  0.00%
 76	28757441	 99.98%
28762802 reads passed initial QC


criterion=sequence-density
sequence-density=96.49
sequence-density-rank=1
fanout-score=23.05
fanout-score-rank=2
prefix-density=97.24
prefix-fanout=22.9
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA


criterion=fanout-score
sequence-density=1.39
sequence-density-rank=6
fanout-score=67.85
fanout-score-rank=1
prefix-density=93.69
prefix-fanout=1.0
sequence=CACGTGAAAATATCGTATGCCGT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGTATGCCGTCTTCTGCTTGAAAAA -o ERR4018152 -
Input file:	STDIN
trimmed:	ERR4018152-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGTATGCCGTCTTCTGCTTGA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Sun Dec 28 15:08:24 2025 >> started

Sun Dec 28 15:08:50 2025 >> done (26.315s)
28169755 reads processed; of these:
 6276888 (22.28%) short reads filtered out after trimming by size control
     391 ( 0.00%) empty reads filtered out after trimming by size control
21892476 (77.72%) reads available; of these:
21552534 (98.45%) trimmed reads available after processing
  339942 ( 1.55%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	 1205834	  5.51%
 19	 1559551	  7.12%
 20	 1116837	  5.10%
 21	 1881588	  8.59%
 22	 2319116	 10.59%
 23	 1639356	  7.49%
 24	  942997	  4.31%
 25	  718146	  3.28%
 26	  384290	  1.76%
 27	  377073	  1.72%
 28	  518553	  2.37%
 29	  454226	  2.07%
 30	 1184032	  5.41%
 31	 3783385	 17.28%
 32	  974141	  4.45%
 33	  367162	  1.68%
 34	  498349	  2.28%
 35	  322082	  1.47%
 36	  112684	  0.51%
 37	   75150	  0.34%
 38	   72539	  0.33%
 39	   68473	  0.31%
 40	   49670	  0.23%
 41	   50408	  0.23%
 42	   57583	  0.26%
 43	   43492	  0.20%
 44	   56214	  0.26%
 45	   36212	  0.17%
 46	   36279	  0.17%
 47	   61011	  0.28%
 48	   44280	  0.20%
 49	  165971	  0.76%
 50	  135389	  0.62%
 51	   23573	  0.11%
 52	   10694	  0.05%
 53	   10115	  0.05%
 54	    9380	  0.04%
 55	    8627	  0.04%
 56	    9635	  0.04%
 57	   18146	  0.08%
 58	   17614	  0.08%
 59	   11388	  0.05%
 60	    8996	  0.04%
 61	   12495	  0.06%
 62	    8429	  0.04%
 63	    7799	  0.04%
 64	    8650	  0.04%
 65	    5883	  0.03%
 66	   12996	  0.06%
 67	    8698	  0.04%
 68	   10197	  0.05%
 69	    4683	  0.02%
 70	    5753	  0.03%
 71	   12007	  0.05%
 72	    7164	  0.03%
 73	    7757	  0.04%
 74	       9	  0.00%
 75	      39	  0.00%
 76	  339676	  1.55%


criterion=sequence-density
sequence-density=11.00
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=8
prefix-density=0.00
prefix-fanout=1.0
sequence=GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCA


criterion=fanout-score
sequence-density=0.25
sequence-density-rank=22
fanout-score=3.66
fanout-score-rank=1
prefix-density=0.91
prefix-fanout=1.0
sequence=TAGTAGGTTGTGTGGTTT
                                 Started job on |	Dec 28 15:09:02
                             Started mapping on |	Dec 28 15:09:02
                                    Finished on |	Dec 28 15:10:13
       Mapping speed, Million of reads per hour |	1140.11

                          Number of input reads |	22485523
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	3514034
                        Uniquely mapped reads % |	15.63%
                          Average mapped length |	25.59
                       Number of splices: Total |	13696
            Number of splices: Annotated (sjdb) |	6151
                       Number of splices: GT/AG |	11091
                       Number of splices: GC/AG |	174
                       Number of splices: AT/AC |	42
               Number of splices: Non-canonical |	2389
                      Mismatch rate per base, % |	0.27%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.05
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.68
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	9364390
             % of reads mapped to multiple loci |	41.65%
        Number of reads mapped to too many loci |	8662151
             % of reads mapped to too many loci |	38.52%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	3.92%
                     % of reads unmapped: other |	0.29%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	9607099	9607099	9607099
N_multimapping	9364390	9364390	9364390
N_noFeature	184265	294404	3397974
N_ambiguous	104292	98368	22
UnstrandedReadsAssigned:3225477 PositiveStrandReadsAssigned:3121262 NegativeStrandReadsAssigned:116038
Dataset is classified positive stranded
MeadianReadLen=25 20thPercentileLength=21 echo kmer=19
ERR4018152 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 31,715
[index] number of k-mers: 49,362,984
[index] number of equivalence classes: 166,181
[quant] running in single-end mode
[quant] will process file 1: ERR4018152-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 22,485,523 reads, 1,966,656 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 819 rounds

  31716 ERR4018152.ke.tsv
  32883 ERR4018152.se.tsv
  64599 total
==> ERR4018152.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	856	321	88.905
ENSRNOT00000040993.4	1039	940	345	87.0134
ENSRNOT00000050156.3	1545	1446	4980	816.5
ENSRNOT00000043693.3	684	585	1798	728.666
ENSRNOT00000046201.3	204	105	92	207.727
ENSRNOT00000046108.3	681	582	573	233.414
ENSRNOT00000049683.3	784	685	742	256.808
ENSRNOT00000041241.3	348	249	115	109.495
ENSRNOT00000044582.3	297	198	120	143.685

==> ERR4018152.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	0
ENSRNOG00000058808	0
ENSRNOG00000061316	2
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	0
ERR4018152 completed mapping pipeline successfully
