Starting /dee2/code/volunteer_pipeline.sh ERR4018147
    current disk space = 375701280
    free memory = 195463168 
ERR4018147 SRAfilesize
0e55735f9380605c5602132d3d449742  ERR4018147.sra
ERR4018147.sra file validated
ERR4018147 is single end
ERR4018147 is conventional basespace
ERR4018147 read1 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR4018147_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7675	35.0	35.0	35.0	27.0	35.0
2	33.892	35.0	35.0	35.0	30.0	35.0
3	34.19075	35.0	35.0	35.0	32.0	35.0
4	34.374	35.0	35.0	35.0	33.0	35.0
5	33.84775	35.0	35.0	35.0	31.0	35.0
6	38.3895	39.0	38.0	40.0	36.0	40.0
7	38.97175	40.0	39.0	40.0	38.0	40.0
8	39.09125	40.0	39.0	40.0	38.0	40.0
9	38.92675	40.0	39.0	40.0	37.0	40.0
10	39.14025	40.0	39.0	40.0	38.0	40.0
11	39.09275	40.0	39.0	40.0	38.0	40.0
12	39.08725	40.0	39.0	40.0	38.0	40.0
13	39.13525	40.0	39.0	40.0	38.0	40.0
14	39.059	40.0	39.0	40.0	38.0	40.0
15	39.01725	40.0	39.0	40.0	38.0	40.0
16	39.0765	40.0	39.0	40.0	38.0	40.0
17	39.11	40.0	39.0	40.0	38.0	40.0
18	39.025	40.0	39.0	40.0	38.0	40.0
19	39.072	40.0	39.0	40.0	38.0	40.0
20	39.06625	40.0	39.0	40.0	38.0	40.0
21	39.15725	40.0	39.0	40.0	38.0	40.0
22	39.10025	40.0	39.0	40.0	38.0	40.0
23	39.041	40.0	39.0	40.0	38.0	40.0
24	39.0435	40.0	39.0	40.0	38.0	40.0
25	39.03775	40.0	39.0	40.0	38.0	40.0
26	39.00575	40.0	39.0	40.0	38.0	40.0
27	39.0865	40.0	39.0	40.0	38.0	40.0
28	39.15525	40.0	39.0	40.0	38.0	40.0
29	39.06	40.0	39.0	40.0	38.0	40.0
30	39.047	40.0	39.0	40.0	38.0	40.0
31	38.902	40.0	39.0	40.0	38.0	40.0
32	39.02025	40.0	39.0	40.0	38.0	40.0
33	39.016	40.0	39.0	40.0	38.0	40.0
34	39.0335	40.0	39.0	40.0	38.0	40.0
35	39.00125	40.0	39.0	40.0	38.0	40.0
36	38.908	40.0	39.0	40.0	38.0	40.0
37	39.03225	40.0	39.0	40.0	38.0	40.0
38	38.92175	40.0	39.0	40.0	38.0	40.0
39	38.9425	40.0	39.0	40.0	38.0	40.0
40	38.88525	40.0	39.0	40.0	37.0	40.0
41	38.937	40.0	39.0	40.0	38.0	40.0
42	38.9265	40.0	39.0	40.0	38.0	40.0
43	38.94825	40.0	39.0	40.0	38.0	40.0
44	39.002	40.0	39.0	40.0	38.0	40.0
45	38.9185	40.0	39.0	40.0	38.0	40.0
46	38.9135	40.0	39.0	40.0	38.0	40.0
47	38.94575	40.0	39.0	40.0	38.0	40.0
48	38.9265	40.0	39.0	40.0	38.0	40.0
49	38.581	40.0	39.0	40.0	36.0	40.0
50	38.72875	40.0	39.0	40.0	37.0	40.0
51	38.28275	39.0	39.0	40.0	35.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	0.0
20	1.0
21	0.0
22	1.0
23	0.0
24	1.0
25	2.0
26	4.0
27	5.0
28	6.0
29	13.0
30	12.0
31	29.0
32	24.0
33	36.0
34	57.0
35	91.0
36	123.0
37	274.0
38	1167.0
39	2153.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.601303639326453	18.68549701249321	34.111895708853886	23.601303639326453
2	46.275	13.950000000000001	28.725	11.05
3	20.925	35.4	13.825000000000001	29.849999999999998
4	18.25	37.45	20.9	23.400000000000002
5	21.025	37.5	14.95	26.525
6	25.85	19.925	41.475	12.75
7	54.775	7.199999999999999	27.474999999999998	10.549999999999999
8	27.650000000000002	46.675	13.975000000000001	11.700000000000001
9	11.799999999999999	39.5	38.625	10.075000000000001
10	50.8	13.950000000000001	25.374999999999996	9.875
11	40.2	42.475	9.75	7.575
12	29.325000000000003	30.55	17.075000000000003	23.05
13	17.65	30.575000000000003	20.474999999999998	31.3
14	11.125	37.475	32.125	19.275000000000002
15	18.175	20.5	32.85	28.475
16	16.475	33.4	31.924999999999997	18.2
17	23.225	24.775	16.975	35.025
18	21.175	32.925	27.474999999999998	18.425
19	16.3	32.375	33.225	18.099999999999998
20	15.075	26.025	30.475	28.425
21	23.474999999999998	15.6	50.724999999999994	10.2
22	32.85	23.0	27.625	16.525000000000002
23	26.900000000000002	32.5	11.25	29.349999999999998
24	30.5	30.15	18.575	20.775
25	30.5	24.875	14.475	30.15
26	23.799999999999997	22.3	25.25	28.65
27	30.3	20.150000000000002	12.7	36.85
28	18.925	19.225	27.474999999999998	34.375
29	27.575	22.475	27.200000000000003	22.75
30	34.2	14.099999999999998	28.925	22.775000000000002
31	24.025	29.525000000000002	26.775	19.675
32	32.5	22.525000000000002	16.575	28.4
33	14.399999999999999	32.2	18.3	35.099999999999994
34	14.099999999999998	18.625	31.0	36.275
35	22.5	16.925	37.55	23.025000000000002
36	24.625	46.025	10.95	18.4
37	22.275	42.65	16.325	18.75
38	20.849999999999998	32.175	12.5	34.475
39	22.675	24.725	36.575	16.025
40	28.65	15.45	30.15	25.75
41	33.35	27.825	20.849999999999998	17.974999999999998
42	20.150000000000002	36.325	31.7	11.825
43	19.825	27.950000000000003	21.725	30.5
44	23.275000000000002	33.7	28.9	14.124999999999998
45	21.2	41.949999999999996	16.650000000000002	20.200000000000003
46	26.8	29.625	19.625	23.95
47	13.725000000000001	28.799999999999997	37.375	20.1
48	25.75	37.025000000000006	20.974999999999998	16.25
49	12.025	35.125	36.925000000000004	15.925
50	24.9	20.8	20.150000000000002	34.150000000000006
51	23.925	20.474999999999998	25.374999999999996	30.225
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	1.0
23	0.5
24	0.0
25	0.5
26	2.5
27	4.0
28	12.5
29	21.0
30	40.0
31	59.0
32	39.0
33	19.0
34	55.5
35	92.0
36	244.0
37	396.0
38	372.0
39	348.0
40	354.0
41	360.0
42	238.0
43	116.0
44	129.5
45	143.0
46	278.0
47	413.0
48	510.0
49	607.0
50	548.0
51	489.0
52	388.5
53	288.0
54	257.5
55	227.0
56	186.0
57	145.0
58	143.5
59	142.0
60	94.0
61	46.0
62	30.5
63	15.0
64	12.0
65	9.0
66	10.5
67	12.0
68	17.5
69	23.0
70	20.0
71	17.0
72	12.0
73	7.0
74	3.5
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	7.95
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.199999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.21546961325967	29.4
2	7.320441988950275	5.3
3	3.1767955801104977	3.45
4	1.4502762430939227	2.1
5	0.9668508287292817	1.7500000000000002
6	1.0359116022099446	2.25
7	0.48342541436464087	1.225
8	0.5524861878453038	1.6
9	0.4143646408839779	1.35
>10	2.7624309392265194	19.950000000000003
>50	0.20718232044198895	5.0
>100	0.4143646408839779	26.625
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC	313	7.825	TruSeq Adapter, Index 10 (100% over 50bp)
TGCACTGATGAAACTAGAAATAGGAAGTGCCGTCCGAAACGACAACTGACG	193	4.825	No Hit
TGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAAACT	184	4.6	No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCT	142	3.55	TruSeq Adapter, Index 10 (100% over 51bp)
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAAAC	119	2.9749999999999996	No Hit
GTAAATGATGAGATTTCCATTGGTCCGTGTTTCTGAACTCCATGATTTTTG	114	2.85	No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATAAAAC	81	2.025	No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG	67	1.675	No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAT	52	1.3	TruSeq Adapter, Index 10 (100% over 41bp)
CACAATGATGACTTATGTTTTTGCCGTTTACCCAGCTGAGGGTTTCTTTGA	43	1.075	No Hit
TACTGTGATGATAACATAGTTCAGCAGACGTGACTCTGGTGAACAATCATG	42	1.05	No Hit
TGGAATGATGACATTCTCCGGAATCGCTGTACTGTCTTGATGAAAGTACTT	41	1.0250000000000001	No Hit
TTGCAATGATGTGAATCTTTTGCTGAAGTGACCTTGAAGTGTAATTCCTGA	40	1.0	No Hit
ATACATGATGATCTCACTCCAACTTGAACTCTCTCACTGATTACTTGATGA	34	0.8500000000000001	No Hit
GGTCTGTGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	34	0.8500000000000001	No Hit
TGCTGTGATGAGGACACCAGTAGGAAGTGCCGTCAGAGTCGATAACTGACG	34	0.8500000000000001	No Hit
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGATGTGGAGAAAGGTA	34	0.8500000000000001	No Hit
CTGCGATGATGTCATTTCTTAGGACACCTTTGGATTAATCATGAAAATAGC	32	0.8	No Hit
NGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC	31	0.775	TruSeq Adapter, Index 10 (100% over 50bp)
GCTAATGATGATACCGCACTAACTGAGCAGTTAGTAGTTGGTCCTTTGATT	27	0.675	No Hit
CTTAATGATGATTGTCTATTTGATTGCTTGATGCAATGTGAAAACCACATT	22	0.5499999999999999	No Hit
GTTCTGTGATGAGACTCTGGAATGTGCGCTGGGCACAGCGCCCGAGTGAGA	20	0.5	No Hit
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAT	19	0.475	TruSeq Adapter, Index 10 (100% over 41bp)
NGCACTGATGAAACTAGAAATAGGAAGTGCCGTCCGAAACGACAACTGACG	18	0.44999999999999996	No Hit
CTTCTGTGATGACTTTACCCAAATGACTTTCGTTCTTCTGAGTTTGCTGAA	17	0.42500000000000004	No Hit
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGTA	17	0.42500000000000004	No Hit
NGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAAACT	16	0.4	No Hit
TTCTGTGATGAGACTCTGGAATGTGCGCTGGGCACAGCGCCCGAGTGAGAC	16	0.4	No Hit
GGTCAATGATGTGTTGGCATGTATTATCTGAATTTGCTGATGTGTCCTAAC	16	0.4	No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG	15	0.375	No Hit
GTGGAATGATGACATTCTCCGGAATCGCTGTACTGTCTTGATGAAAGTACT	15	0.375	No Hit
ATCGATGTGGTGCTGCCGCGTTCTCTTCGGGCCGGGGCCTAAGCCGCGCCA	15	0.375	No Hit
TTGAGTGATGACTTGTATTGTCGGATACCCCTTCACCCCGTTCATGGGTGA	15	0.375	No Hit
AGTAAATGATGAGATTTCCATTGGTCCGTGTTTCTGAACTCCATGATTTTT	15	0.375	No Hit
GCGGATGATGACACCTGGGTATGCTGCACATTCCCGACTGCGTCGTGGGGA	14	0.35000000000000003	No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG	13	0.325	No Hit
NGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCT	13	0.325	TruSeq Adapter, Index 10 (98% over 51bp)
NTAAATGATGAGATTTCCATTGGTCCGTGTTTCTGAACTCCATGATTTTTG	12	0.3	No Hit
TTTGAGTGATGACTTGTATTGTCGGATACCCCTTCACCCCGTTCATGGGTG	12	0.3	No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCCA	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTCC	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATCGATGATGTCCTAAAAGTAAATGTCTGAACATATGAATGCTATACTGA	11	0.27499999999999997	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCAC	11	0.27499999999999997	No Hit
CTCCCATGATGTATAGCACTGGGCTCTGACCACCATGGTGACGTGTTGTGC	11	0.27499999999999997	No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA	10	0.25	No Hit
CACAAATGATGAATCCTTTGACGGGCGGGCAGAATCTCTGTGCTGATTGTC	10	0.25	No Hit
CTTCGGTGATGACACGATGACGAGTCAGAAAGGCCACGTCTTGCTCTTGGG	10	0.25	No Hit
TATGGCACTGGTAGAATTCACTAGATCGGAAGAGCACACGTCTGAACTCCA	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACAGTGATGACCACAACATGCCATCTGAGTTGCTTTGCTGAAATCCAGAG	9	0.22499999999999998	No Hit
TCGATGTGGTGCTGCCGCGTTCTCTTCGGGCCGGGGCCTAAGCCGCGCCAG	9	0.22499999999999998	No Hit
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT	9	0.22499999999999998	TruSeq Adapter, Index 10 (100% over 39bp)
AGATCGGAAGAGCACACGTCTAAACTCCAGTCACTAGCTTATCTCGTATGC	9	0.22499999999999998	TruSeq Adapter, Index 10 (98% over 50bp)
TGTCCCATTTGACTACCACATTTCCTACTGATAATAGATTTTGGTGGCTAT	9	0.22499999999999998	No Hit
CTACAATGATGGTAATATTTTTCGTCAACAGAGTTCACCTAGTGAGTGTTG	9	0.22499999999999998	No Hit
ACAAGTGATGACTAACAAAGGGACTTAATACTGAAACATCTGGGATTGAAA	8	0.2	No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCAGATCGGAAGAGCACACGTCTGA	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC	8	0.2	No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGTC	8	0.2	No Hit
NTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAAAC	8	0.2	No Hit
TGCAATGATGAAGGTTATGTTGGTAGGGACAACTGACCTTGTTGATGAATA	8	0.2	No Hit
TGAAGTGATGATTCACAATCATGTCTCTTCTCTGAAGAATTCTTGGAGATT	8	0.2	No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	8	0.2	No Hit
GTGCAATGATGTGTGAATTTCTTCACCTGAAATCACCGTGAAGAGTAATTT	7	0.17500000000000002	No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCCA	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATATGATGAGCATACAAAATGTTGATTACAGTGAAAAACAGTAAGATCC	7	0.17500000000000002	No Hit
NTACATGATGATCTCACTCCAACTTGAACTCTCTCACTGATTACTTGATGA	7	0.17500000000000002	No Hit
CGTTGGATCGATGTGGTGCTGCCGCGTTCTCTTCGGGCCGGGGCCTAAGCC	7	0.17500000000000002	No Hit
CCGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTA	7	0.17500000000000002	TruSeq Adapter, Index 10 (100% over 40bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTAGATCGGAAGAGCACACGT	7	0.17500000000000002	No Hit
ATGTTGTGATGATGGGCAAAATGTTCAACTGCTCTGAATGGGCTGAATGAA	6	0.15	No Hit
NACAATGATGACTTATGTTTTTGCCGTTTACCCAGCTGAGGGTTTCTTTGA	6	0.15	No Hit
NTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATAAAAC	6	0.15	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA	6	0.15	No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGTCT	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGAGATCGGAAGAGCACACGT	6	0.15	No Hit
ACTTAATGATGATTGTCTATTTGATTGCTTGATGCAATGTGAAAACCACAT	6	0.15	No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATAAGT	6	0.15	No Hit
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGAGATGATGAACTGACTCAACCTGACCTTCAGAAATGGAGGTGATAAAA	6	0.15	No Hit
GATCTATGATGATCCTATCCCGAATCTGAATTCCTGTTGAAAAAATATAAT	6	0.15	No Hit
CCGATACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC	5	0.125	No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTA	5	0.125	No Hit
TGGCGGTGATGACCCACTTGCCCTCACTGAGGACAAAGTTCGTTGGTGAGA	5	0.125	No Hit
CGCGACCTCAGATCAGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
NTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG	5	0.125	No Hit
NGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAT	5	0.125	TruSeq Adapter, Index 10 (100% over 41bp)
ACAAATGATGAATCCTTTGACGGGCGGGCAGAATCTCTGTGCTGATTGTCA	5	0.125	No Hit
ATACTGTGATGATAACATAGTTCAGCAGACGTGACTCTGGTGAACAATCAT	5	0.125	No Hit
GCACAATGATGACTTATGTTTTTGCCGTTTACCCAGCTGAGGGTTTCTTTG	5	0.125	No Hit
AACCCGTAGATCCGATCTTGTGAAGATCGGAAGAGCACACGTCTGAACTCC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCCTGTGATGTTCAGCACTGGGCTCTGATCACCCTGAGGACATGGAGCT	5	0.125	No Hit
CCTAGTGATGACAAGGAGACATTGTCAACCAATCCCCACAAGGGAATGAGG	5	0.125	No Hit
TTTCTGTGATGAATCAACTAGCTCACTATGACTGCTAATGAAAACACTGGA	5	0.125	No Hit
TGCAATGATGTGTGAATTTCTTCACCTGAAATCACCGTGAAGAGTAATTTA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.625	0.0	0.0	0.0	0.0
2	8.675	0.0	0.0	0.0	0.0
3	8.725	0.0	0.0	0.0	0.0
4	8.75	0.0	0.0	0.0	0.0
5	8.825	0.0	0.0	0.0	0.0
6	9.0	0.0	0.0	0.0	0.0
7	9.05	0.0	0.0	0.0	0.0
8	9.275	0.0	0.0	0.0	0.0
9	9.525	0.0	0.0	0.0	0.0
10	12.075	0.0	0.0	0.0	0.0
11	12.675	0.0	0.0	0.0	0.0
12	13.325	0.0	0.0	0.0	0.0
13	13.875	0.0	0.0	0.0	0.0
14	14.55	0.0	0.0	0.0	0.0
15	15.05	0.0	0.0	0.0	0.0
16	15.55	0.0	0.0	0.0	0.0
17	16.075	0.0	0.0	0.0	0.0
18	16.7	0.0	0.0	0.0	0.0
19	17.375	0.0	0.0	0.0	0.0
20	17.85	0.0	0.0	0.0	0.0
21	18.875	0.0	0.0	0.0	0.0
22	20.4	0.0	0.0	0.0	0.0
23	23.55	0.0	0.0	0.0	0.0
24	25.7	0.0	0.0	0.0	0.0
25	26.925	0.0	0.0	0.0	0.0
26	27.65	0.0	0.0	0.0	0.0
27	28.275	0.0	0.0	0.0	0.0
28	28.5	0.0	0.0	0.0	0.0
29	29.375	0.0	0.0	0.0	0.0
30	29.85	0.0	0.0	0.0	0.0
31	30.3	0.0	0.0	0.0	0.0
32	31.55	0.0	0.0	0.0	0.0
33	32.275	0.0	0.0	0.0	0.0
34	32.675	0.0	0.0	0.0	0.0
35	33.025	0.0	0.0	0.0	0.0
36	33.5	0.0	0.0	0.0	0.0
37	34.1	0.0	0.0	0.0	0.0
38	34.525	0.0	0.0	0.0	0.0
39	34.75	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGACC	15	0.007837298	49.88889	1
CTCGCTG	30	7.9634447E-7	49.88889	1
GTAAATG	15	0.007837298	49.88889	1
TGATGAG	35	8.720781E-8	44.9	6
CATGATT	20	6.2916183E-4	44.9	41
TGGTCCG	20	6.2916183E-4	44.9	21
GTGTTTC	20	6.2916183E-4	44.9	27
CCGTGTT	20	6.2916183E-4	44.9	25
TAAATGA	20	6.2916183E-4	44.9	2
TAGGAAG	20	6.2916183E-4	44.9	21
ATGATTT	20	6.2916183E-4	44.9	42
ACTGACG	20	6.2916183E-4	44.9	45
TTTCTGA	20	6.2916183E-4	44.9	30
TTTCCAT	20	6.2916183E-4	44.9	14
GTCCGTG	20	6.2916183E-4	44.9	23
GGTCCGT	20	6.2916183E-4	44.9	22
TGATTTT	20	6.2916183E-4	44.9	43
GAAGTGC	20	6.2916183E-4	44.9	24
ATTGGTC	20	6.2916183E-4	44.9	19
CTCCATG	20	6.2916183E-4	44.9	38
>>END_MODULE
Rejected 2325203 READS because READLEN < 1
Read 2325203 spots for ERR4018147.sra
Written 2325203 spots for ERR4018147.sra
Rejected 2325210 READS because READLEN < 1
Read 2325210 spots for ERR4018147.sra
Written 2325210 spots for ERR4018147.sra
Rejected 2325203 READS because READLEN < 1
Read 2325203 spots for ERR4018147.sra
Written 2325203 spots for ERR4018147.sra
Rejected 2325203 READS because READLEN < 1
Read 2325203 spots for ERR4018147.sra
Written 2325203 spots for ERR4018147.sra
Rejected 2325203 READS because READLEN < 1
Read 2325203 spots for ERR4018147.sra
Written 2325203 spots for ERR4018147.sra
Rejected 2325203 READS because READLEN < 1
Read 2325203 spots for ERR4018147.sra
Written 2325203 spots for ERR4018147.sra
Rejected 2325203 READS because READLEN < 1
Read 2325203 spots for ERR4018147.sra
Written 2325203 spots for ERR4018147.sra
Rejected 2325203 READS because READLEN < 1
Read 2325203 spots for ERR4018147.sra
Written 2325203 spots for ERR4018147.sra
SRR ids: ['ERR4018147.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_voy9ypst
ERR4018147.sra spots: 18601631
blocks: [[1, 2325203], [2325204, 4650406], [4650407, 6975609], [6975610, 9300812], [9300813, 11626015], [11626016, 13951218], [13951219, 16276421], [16276422, 18601631]]
ERR4018147 file size 2630492
ERR4018147 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o ERR4018147 ERR4018147_1.fastq
Input file:	ERR4018147_1.fastq
trimmed:	ERR4018147-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Sun Dec 28 15:04:41 2025 >> started

Sun Dec 28 15:04:46 2025 >> done (4.939s)
18601631 reads processed; of these:
 1495893 ( 8.04%) short reads filtered out after trimming by size control
 1755662 ( 9.44%) empty reads filtered out after trimming by size control
15350076 (82.52%) reads available; of these:
  237470 ( 1.55%) trimmed reads available after processing
15112606 (98.45%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  125054	  0.81%
 19	  112338	  0.73%
 20	       0	  0.00%
 21	       0	  0.00%
 22	       0	  0.00%
 23	       0	  0.00%
 24	       0	  0.00%
 25	       0	  0.00%
 26	       0	  0.00%
 27	       0	  0.00%
 28	       0	  0.00%
 29	       0	  0.00%
 30	       0	  0.00%
 31	       0	  0.00%
 32	       0	  0.00%
 33	       0	  0.00%
 34	       0	  0.00%
 35	       0	  0.00%
 36	       0	  0.00%
 37	       0	  0.00%
 38	       0	  0.00%
 39	       0	  0.00%
 40	       0	  0.00%
 41	       0	  0.00%
 42	       0	  0.00%
 43	       0	  0.00%
 44	       0	  0.00%
 45	       0	  0.00%
 46	       0	  0.00%
 47	       0	  0.00%
 48	       0	  0.00%
 49	      36	  0.00%
 50	      42	  0.00%
 51	15112606	 98.45%
15350076 reads passed initial QC


criterion=sequence-density
sequence-density=19.88
sequence-density-rank=1
fanout-score=35.95
fanout-score-rank=1
prefix-density=20.69
prefix-fanout=34.6
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTC


criterion=fanout-score
sequence-density=19.88
sequence-density-rank=1
fanout-score=35.95
fanout-score-rank=1
prefix-density=20.69
prefix-fanout=34.6
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTC -o ERR4018147 -
Input file:	STDIN
trimmed:	ERR4018147-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Sun Dec 28 15:04:56 2025 >> started

Sun Dec 28 15:05:04 2025 >> done (8.291s)
13815069 reads processed; of these:
    7121 ( 0.05%) short reads filtered out after trimming by size control
    2630 ( 0.02%) empty reads filtered out after trimming by size control
13805318 (99.93%) reads available; of these:
 3992300 (28.92%) trimmed reads available after processing
 9813018 (71.08%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  108958	  0.79%
 19	  104755	  0.76%
 20	  173993	  1.26%
 21	  234181	  1.70%
 22	  525659	  3.81%
 23	  342166	  2.48%
 24	  237315	  1.72%
 25	  104423	  0.76%
 26	   82586	  0.60%
 27	   84806	  0.61%
 28	  115037	  0.83%
 29	   65141	  0.47%
 30	  105170	  0.76%
 31	  187488	  1.36%
 32	  123967	  0.90%
 33	   74195	  0.54%
 34	   60124	  0.44%
 35	   58326	  0.42%
 36	   91810	  0.67%
 37	   62213	  0.45%
 38	   42182	  0.31%
 39	   42995	  0.31%
 40	   28864	  0.21%
 41	   28061	  0.20%
 42	   42108	  0.31%
 43	   83904	  0.61%
 44	  126583	  0.92%
 45	  520354	  3.77%
 46	   82437	  0.60%
 47	   36810	  0.27%
 48	  223875	  1.62%
 49	      18	  0.00%
 50	      24	  0.00%
 51	 9604790	 69.57%


criterion=sequence-density
sequence-density=4.36
sequence-density-rank=1
fanout-score=1.96
fanout-score-rank=11
prefix-density=0.02
prefix-fanout=2.0
sequence=GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAAACTCTCTGCTGATC


criterion=fanout-score
sequence-density=0.44
sequence-density-rank=14
fanout-score=43.57
fanout-score-rank=1
prefix-density=5.79
prefix-fanout=3.3
sequence=GTGATGACTTTC
                                 Started job on |	Dec 28 15:05:13
                             Started mapping on |	Dec 28 15:05:13
                                    Finished on |	Dec 28 15:05:39
       Mapping speed, Million of reads per hour |	2124.05

                          Number of input reads |	15340325
                      Average input read length |	45
                                    UNIQUE READS:
                   Uniquely mapped reads number |	10248797
                        Uniquely mapped reads % |	66.81%
                          Average mapped length |	47.48
                       Number of splices: Total |	12660
            Number of splices: Annotated (sjdb) |	2738
                       Number of splices: GT/AG |	11725
                       Number of splices: GC/AG |	210
                       Number of splices: AT/AC |	3
               Number of splices: Non-canonical |	722
                      Mismatch rate per base, % |	0.37%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.09
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.23
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	3135667
             % of reads mapped to multiple loci |	20.44%
        Number of reads mapped to too many loci |	462199
             % of reads mapped to too many loci |	3.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	9.64%
                     % of reads unmapped: other |	0.10%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1955861	1955861	1955861
N_multimapping	3135667	3135667	3135667
N_noFeature	324613	422992	9842223
N_ambiguous	858199	549938	66
UnstrandedReadsAssigned:9065985 PositiveStrandReadsAssigned:9275867 NegativeStrandReadsAssigned:406508
Dataset is classified positive stranded
MeadianReadLen=51 20thPercentileLength=35 echo kmer=31
ERR4018147 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 31,715
[index] number of k-mers: 49,915,185
[index] number of equivalence classes: 70,351
[quant] running in single-end mode
[quant] will process file 1: ERR4018147-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 15,340,325 reads, 105,086 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 679 rounds

  31716 ERR4018147.ke.tsv
  32883 ERR4018147.se.tsv
  64599 total
==> ERR4018147.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	856	118	906.33
ENSRNOT00000040993.4	1039	940	159	1112.11
ENSRNOT00000050156.3	1545	1446	855	3887.55
ENSRNOT00000043693.3	684	585	421	4731.56
ENSRNOT00000046201.3	204	105	154	9642.94
ENSRNOT00000046108.3	681	582	151	1705.82
ENSRNOT00000049683.3	784	685	119	1142.18
ENSRNOT00000041241.3	348	249	35	924.159
ENSRNOT00000044582.3	297	198	105	3486.6

==> ERR4018147.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	0
ENSRNOG00000058808	0
ENSRNOG00000061316	2
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	0
ERR4018147 completed mapping pipeline successfully
