Starting /dee2/code/volunteer_pipeline.sh ERR4018151
    current disk space = 372092948
    free memory = 195692288 
ERR4018151 SRAfilesize
600196bc4e477ce12d3690f5b897c33e  ERR4018151.sra
ERR4018151.sra file validated
ERR4018151 is single end
ERR4018151 is conventional basespace
ERR4018151 read1 length is 76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR4018151_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	76
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3815	32.0	32.0	32.0	32.0	32.0
2	31.41125	32.0	32.0	32.0	32.0	32.0
3	31.642	32.0	32.0	32.0	32.0	32.0
4	31.644	32.0	32.0	32.0	32.0	32.0
5	31.6235	32.0	32.0	32.0	32.0	32.0
6	34.9615	36.0	36.0	36.0	36.0	36.0
7	35.2355	36.0	36.0	36.0	36.0	36.0
8	35.199	36.0	36.0	36.0	36.0	36.0
9	35.2165	36.0	36.0	36.0	36.0	36.0
10-11	35.346000000000004	36.0	36.0	36.0	36.0	36.0
12-13	35.142624999999995	36.0	36.0	36.0	36.0	36.0
14-15	35.19775	36.0	36.0	36.0	36.0	36.0
16-17	35.291125	36.0	36.0	36.0	36.0	36.0
18-19	35.213625	36.0	36.0	36.0	36.0	36.0
20-21	35.177499999999995	36.0	36.0	36.0	36.0	36.0
22-23	35.20425	36.0	36.0	36.0	36.0	36.0
24-25	35.122375000000005	36.0	36.0	36.0	36.0	36.0
26-27	35.0085	36.0	36.0	36.0	36.0	36.0
28-29	34.9555	36.0	36.0	36.0	36.0	36.0
30-31	34.972375	36.0	36.0	36.0	36.0	36.0
32-33	35.133	36.0	36.0	36.0	36.0	36.0
34-35	35.143375000000006	36.0	36.0	36.0	36.0	36.0
36-37	35.067375	36.0	36.0	36.0	36.0	36.0
38-39	35.132000000000005	36.0	36.0	36.0	36.0	36.0
40-41	34.883125	36.0	36.0	36.0	34.0	36.0
42-43	35.069625	36.0	36.0	36.0	36.0	36.0
44-45	35.036500000000004	36.0	36.0	36.0	36.0	36.0
46-47	34.929375	36.0	36.0	36.0	36.0	36.0
48-49	34.678875000000005	36.0	36.0	36.0	32.0	36.0
50-51	34.656625000000005	36.0	36.0	36.0	32.0	36.0
52-53	34.43475	36.0	36.0	36.0	32.0	36.0
54-55	33.991749999999996	36.0	36.0	36.0	29.5	36.0
56-57	33.600875	36.0	34.0	36.0	27.0	36.0
58-59	32.958749999999995	36.0	32.0	36.0	24.0	36.0
60-61	32.933375	36.0	32.0	36.0	27.0	36.0
62-63	32.332625	36.0	32.0	36.0	21.0	36.0
64-65	32.643	36.0	32.0	36.0	21.0	36.0
66-67	33.377875	36.0	36.0	36.0	27.0	36.0
68-69	33.472125	36.0	36.0	36.0	27.0	36.0
70-71	33.19725	36.0	36.0	36.0	24.0	36.0
72-73	33.213499999999996	36.0	36.0	36.0	24.0	36.0
74-75	33.541875000000005	36.0	36.0	36.0	27.0	36.0
76	32.03925	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	5.0
22	4.0
23	3.0
24	10.0
25	22.0
26	22.0
27	56.0
28	50.0
29	58.0
30	106.0
31	141.0
32	170.0
33	312.0
34	857.0
35	2182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.225	23.474999999999998	17.424999999999997	22.875
2	35.85	36.325	9.675	18.15
3	19.35	27.275	10.05	43.325
4	32.375	11.825	36.3	19.5
5	19.775000000000002	25.55	28.625	26.05
6	22.163522012578614	13.0062893081761	35.82389937106918	29.006289308176104
7	27.575	18.85	32.225	21.349999999999998
8	17.4	25.775	47.875	8.95
9	36.5	13.975000000000001	20.7	28.825
10-11	44.0875	18.2	19.112499999999997	18.6
12-13	26.787499999999998	23.95	29.9	19.3625
14-15	40.6875	23.4875	19.225	16.6
16-17	30.062499999999996	28.15	33.35	8.4375
18-19	40.112500000000004	16.675	25.3	17.9125
20-21	31.887500000000003	18.325	36.65	13.1375
22-23	27.8375	23.7625	32.2875	16.1125
24-25	36.275	18.862499999999997	29.7	15.162500000000001
26-27	19.787499999999998	26.150000000000002	30.875000000000004	23.1875
28-29	28.069077712426484	13.665373545238394	35.802778125391065	22.462770616944063
30-31	25.25	8.05	32.2875	34.4125
32-33	43.6125	10.5	22.7125	23.175
34-35	46.5375	17.5125	17.6125	18.337500000000002
36-37	34.5875	23.2625	23.9	18.25
38-39	23.799999999999997	24.4	16.625	35.175
40-41	23.1125	35.449999999999996	13.5375	27.900000000000002
42-43	41.3875	26.950000000000003	12.5	19.162499999999998
44-45	25.112499999999997	31.9625	15.662499999999998	27.2625
46-47	20.325	21.05	20.4375	38.1875
48-49	15.9125	25.7625	15.812499999999998	42.512499999999996
50-51	28.15	26.25	14.799999999999999	30.8
52-53	22.400000000000002	24.8	20.1375	32.6625
54-55	19.7375	24.6625	15.525	40.075
56-57	20.1125	14.149999999999999	24.725	41.0125
58-59	18.875	13.9375	18.65	48.5375
60-61	22.1	15.2125	22.9875	39.7
62-63	16.1	20.962500000000002	29.925	33.0125
64-65	16.375	12.3375	33.725	37.5625
66-67	25.275	9.55	29.549999999999997	35.625
68-69	24.349999999999998	15.85	30.55	29.25
70-71	22.825	14.0375	30.575000000000003	32.5625
72-73	20.775	7.775	30.9875	40.4625
74-75	18.475	7.6499999999999995	38.5	35.375
76	22.0	7.55	41.25	29.2
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.5
29	1.0
30	1.5
31	1.5
32	2.0
33	3.0
34	2.0
35	2.0
36	2.5
37	2.0
38	3.0
39	4.0
40	5.0
41	8.0
42	10.0
43	11.0
44	9.0
45	5.5
46	5.0
47	14.5
48	31.5
49	100.5
50	162.0
51	269.5
52	469.5
53	549.0
54	536.0
55	665.0
56	657.5
57	449.0
58	377.0
59	331.0
60	183.5
61	97.0
62	112.0
63	82.0
64	28.5
65	2.5
66	0.5
67	1.0
68	0.5
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	1.0
87	2.0
88	1.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	3.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.625
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.11249999999999999
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.75
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.65034965034965	28.475
2	7.4825174825174825	5.35
3	2.6573426573426575	2.85
4	1.8881118881118881	2.7
5	0.9790209790209791	1.7500000000000002
6	1.118881118881119	2.4
7	0.5594405594405595	1.4000000000000001
8	0.6293706293706294	1.7999999999999998
9	0.7692307692307693	2.475
>10	3.5664335664335662	24.65
>50	0.4195804195804196	9.85
>100	0.27972027972027974	16.3
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GACTCTTAGCGGTGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	229	5.7250000000000005	RNA PCR Primer, Index 1 (100% over 33bp)
ACAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	178	4.45	RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	127	3.175	No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA	118	2.9499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	100	2.5	RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTCAGATCAGACGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	75	1.875	RNA PCR Primer, Index 1 (100% over 30bp)
GACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	58	1.4500000000000002	RNA PCR Primer, Index 1 (100% over 32bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCTGGAATTCTCGGGTGC	58	1.4500000000000002	No Hit
GACTCTTAGCGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTC	52	1.3	RNA PCR Primer, Index 1 (97% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA	51	1.275	No Hit
AGCTACATTGTCTGCTGGGTTTCTGGAATTCTCGGGTGCCAAGGAACTCC	47	1.175	RNA PCR Primer, Index 1 (100% over 27bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGTGGAATTCTCGGGTGCCAAG	40	1.0	No Hit
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	40	1.0	RNA PCR Primer, Index 1 (100% over 29bp)
TGGCTCAGTTCAGCAGGAACAGTGGAATTCTCGGGTGCCAAGGAACTCCA	38	0.95	RNA PCR Primer, Index 1 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	37	0.9249999999999999	No Hit
CGCGACCTCAGATCAGACGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 29bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCTGGAATTCTCGGGTG	31	0.775	No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG	28	0.7000000000000001	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	28	0.7000000000000001	No Hit
CAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	26	0.65	RNA PCR Primer, Index 1 (100% over 30bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA	26	0.65	No Hit
AACATTCATTGCTGTCGGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTC	25	0.625	RNA PCR Primer, Index 1 (100% over 26bp)
GACTCTTAGCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT	24	0.6	RNA PCR Primer, Index 1 (97% over 35bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	23	0.575	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAG	22	0.5499999999999999	No Hit
CGCGACCTCAGATCAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	21	0.525	RNA PCR Primer, Index 1 (100% over 32bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	20	0.5	No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC	19	0.475	RNA PCR Primer, Index 1 (100% over 27bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGTGGAATTCTCGGGTGCCAA	19	0.475	No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTC	18	0.44999999999999996	RNA PCR Primer, Index 1 (97% over 36bp)
ACAGTAGTCTGCACATTGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
GACTCTTAGCGGTGGATCACTTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
TGGATTTTTGGAACTAGGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 29bp)
GACTCTTAGCGGTGGATCACTCTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA	16	0.4	RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC	15	0.375	RNA PCR Primer, Index 1 (100% over 27bp)
ATCCCGGGGCCGAGGGAGCCGGATTGGAATTCTCGGGTGCCAAGGAACTC	15	0.375	RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 28bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTTGGAATTCTCGGGTGCCAAGG	14	0.35000000000000003	Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCT	13	0.325	RNA PCR Primer, Index 18 (100% over 43bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC	13	0.325	No Hit
TGGAAGACTAGTGATTTTGTTGTTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCTGGAATTCTCGGGTGCCAA	13	0.325	No Hit
CGCGACCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCAT	12	0.3	RNA PCR Primer, Index 18 (100% over 41bp)
AATCCCGGGGCCGAGGGAGCCGGATTGGAATTCTCGGGTGCCAAGGAACT	12	0.3	RNA PCR Primer, Index 1 (100% over 25bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA	12	0.3	RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT	11	0.27499999999999997	RNA PCR Primer, Index 1 (97% over 35bp)
GGTGCGGACCAGGGGAATCCGACTGTTTGGAATTCTCGGGTGCCAAGGAA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 23bp)
CGACTCTTAGCGGTGGATCACTCTGGAATTCTCGGGTGCCAAGGAACTCC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 27bp)
AATCCCGGGGCCGAGGGAGCCGGATGGAATTCTCGGGTGCCAAGGAACTC	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 26bp)
GAGGTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAGGAACT	10	0.25	RNA PCR Primer, Index 1 (100% over 25bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG	10	0.25	No Hit
GGCCGCCGGTGAAATACCACTACTCTCATGGAATTCTCGGGTGCCAAGGA	10	0.25	RNA PCR Primer, Index 1 (100% over 22bp)
GTTTCCGTAGTGTAGTGGTTATCTGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
ATCCCGGGGCCGAGGGAGCCGGATGGAATTCTCGGGTGCCAAGGAACTCC	10	0.25	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
GACTCTTAGCGGTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	9	0.22499999999999998	RNA PCR Primer, Index 6 (100% over 34bp)
AGAGGTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAGGAAC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 24bp)
CGACTCTTAGCGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (97% over 35bp)
ACAGTAGTCTGCACATTGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCTGGAATTCTCGGGTGCCA	9	0.22499999999999998	No Hit
ACAGTAGTCTGCACATTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	9	0.22499999999999998	No Hit
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (97% over 36bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTGGAATTCTCGGGTGCCA	9	0.22499999999999998	No Hit
TACAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCTGGAATTCTCGGGTGCCAAGGA	8	0.2	RNA PCR Primer, Index 1 (100% over 22bp)
ATGGATTTTTGGAACTAGGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA	8	0.2	RNA PCR Primer, Index 1 (100% over 23bp)
TGAGGTAGTAGTTTGTGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	8	0.2	RNA PCR Primer, Index 1 (100% over 29bp)
GGCCGCCGGTGAAATACCACTACTCTGGAATTCTCGGGTGCCAAGGAACT	8	0.2	RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AATGGATTTTTGGAACTAGGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
GGTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCATGACAGAACTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
GGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCAAGG	7	0.17500000000000002	Illumina Small RNA Adapter 2 (100% over 21bp)
CGACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAAG	7	0.17500000000000002	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA	7	0.17500000000000002	No Hit
GGCTGGTCCGAAGGTAGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCATG	6	0.15	No Hit
AGCTACATTGTCTGCTGGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
AGGTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
AAATGGATTTTTGGAACTAGGAGTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	6	0.15	RNA PCR Primer, Index 1 (100% over 33bp)
GTTTCCGTAGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCG	6	0.15	RNA PCR Primer, Index 18 (100% over 38bp)
GTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG	6	0.15	RNA PCR Primer, Index 1 (100% over 29bp)
CGACTCTTAGCGGTGGATCACTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GACTCTTAGCGGTGGATCATGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTTGGAATTCTCGGGTGCCAAG	6	0.15	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	6	0.15	No Hit
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CGACTCTTAGCGGTGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	6	0.15	RNA PCR Primer, Index 1 (100% over 32bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
CACCCGTAGAACCGACCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
CATGAGAGGTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
AGGCAGTGTAGTTAGCTGATTGCTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
CTTCTCACTACTGCACTTGACTAGTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
TGGAAGACTAGTGATTTTGTTGTTTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCG	5	0.125	RNA PCR Primer, Index 18 (100% over 38bp)
CGACTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATC	5	0.125	RNA PCR Primer, Index 18 (100% over 42bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCATGG	5	0.125	No Hit
ACTAACCCTAGCCCTACAACCAACCTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
CGGTGGATCACTCGGCTCGTGCGTCTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTTGGAATTCTCGGGTGCC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.025	0.0	0.0	0.0
6	0.0	0.05	0.0	0.0	0.0
7	0.0	0.175	0.0	0.0	0.0
8	0.0	1.175	0.0	0.0	0.0
9	0.0	1.75	0.0	0.0	0.0
10	0.0	2.5	0.0	0.0	0.0
11	0.0	3.0	0.0	0.0	0.0
12	0.0	3.45	0.0	0.0	0.0
13	0.0	4.25	0.0	0.0	0.0
14	0.0	4.875	0.0	0.0	0.0
15	0.0	8.025	0.0	0.0	0.0
16	0.0	9.775	0.0	0.0	0.0
17	0.0	11.15	0.0	0.0	0.0
18	0.0	18.025	0.0	0.0	0.0
19	0.0	21.025	0.0	0.0	0.0
20	0.0	25.275	0.0	0.0	0.0
21	0.0	29.75	0.0	0.0	0.0
22	0.0	40.525	0.0	0.0	0.0
23	0.0	53.025	0.0	0.0	0.0
24	0.0	60.75	0.0	0.0	0.0
25	0.0	64.6	0.0	0.0	0.0
26	0.0	66.8	0.0	0.0	0.0
27	0.0	68.15	0.0	0.0	0.0
28	0.0	69.6	0.0	0.0	0.0
29	0.0	71.325	0.0	0.0	0.0
30	0.0	72.725	0.0	0.0	0.0
31	0.0	76.375	0.0	0.0	0.0
32	0.0	84.075	0.0	0.0	0.0
33	0.0	86.35	0.0	0.0	0.0
34	0.0	87.85	0.0	0.0	0.0
35	0.0	89.875	0.0	0.0	0.0
36	0.0	91.1	0.0	0.0	0.0
37	0.0	91.55	0.0	0.0	0.0
38	0.0	91.8	0.0	0.0	0.0
39	0.0	92.0	0.0	0.0	0.0
40	0.0	92.25	0.0	0.0	0.0
41	0.0	92.4	0.0	0.0	0.0
42	0.0	92.575	0.0	0.0	0.0
43	0.0	92.975	0.0	0.0	0.0
44	0.0	93.075	0.0	0.0	0.0
45	0.0	93.35	0.0	0.0	0.0
46	0.0	93.525	0.0	0.0	0.0
47	0.0	93.8	0.0	0.0	0.0
48	0.0	94.075	0.0	0.0	0.0
49	0.0	94.325	0.0	0.0	0.0
50	0.0	94.85	0.0	0.0	0.0
51	0.0	95.2	0.0	0.0	0.0
52	0.0	95.25	0.0	0.0	0.0
53	0.0	95.25	0.0	0.0	0.0
54	0.0	95.275	0.0	0.0	0.0
55	0.0	95.3	0.0	0.0	0.0
56	0.0	95.35	0.0	0.0	0.0
57	0.0	95.4	0.0	0.0	0.0
58	0.0	95.45	0.0	0.0	0.0
59	0.0	95.575	0.0	0.0	0.0
60	0.0	95.625	0.0	0.0	0.0
61	0.0	95.7	0.0	0.0	0.0
62	0.0	95.8	0.0	0.0	0.0
63	0.0	95.85	0.0	0.0	0.0
64	0.0	95.925	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGAAT	15	0.0020658788	70.00001	18
TGGTTCA	15	0.0020658788	70.00001	8
AATCCCG	15	0.0020658788	70.00001	1
AGCTTAT	35	2.6138878E-9	70.00001	2
ACTGATG	30	7.878043E-8	70.00001	12
TGGTAGA	15	0.0020658788	70.00001	16
GATGTTG	30	7.878043E-8	70.00001	15
ATTGGTG	15	0.0020658788	70.00001	3
AGTGGTA	15	0.0020658788	70.00001	14
TAGCTTA	35	2.6138878E-9	70.00001	1
TTATCAG	35	2.6138878E-9	70.00001	5
GGTTCAG	15	0.0020658788	70.00001	9
ACCCGCT	15	0.0020658788	70.00001	25
CTGATGT	30	7.878043E-8	70.00001	13
GTTCAGT	15	0.0020658788	70.00001	10
AGAATTC	15	0.0020658788	70.00001	20
TCAGTGG	15	0.0020658788	70.00001	12
TCTCGCC	15	0.0020658788	70.00001	25
GCTCGTG	30	7.878043E-8	70.00001	24
TTCTCGC	15	0.0020658788	70.00001	24
>>END_MODULE
Rejected 2550708 READS because READLEN < 1
Read 2550708 spots for ERR4018151.sra
Written 2550708 spots for ERR4018151.sra
Rejected 2550708 READS because READLEN < 1
Read 2550708 spots for ERR4018151.sra
Written 2550708 spots for ERR4018151.sra
Rejected 2550708 READS because READLEN < 1
Read 2550708 spots for ERR4018151.sra
Written 2550708 spots for ERR4018151.sra
Rejected 2550708 READS because READLEN < 1
Read 2550708 spots for ERR4018151.sra
Written 2550708 spots for ERR4018151.sra
Rejected 2550708 READS because READLEN < 1
Read 2550708 spots for ERR4018151.sra
Written 2550708 spots for ERR4018151.sra
Rejected 2550708 READS because READLEN < 1
Read 2550708 spots for ERR4018151.sra
Written 2550708 spots for ERR4018151.sra
Rejected 2550708 READS because READLEN < 1
Read 2550708 spots for ERR4018151.sra
Written 2550708 spots for ERR4018151.sra
Rejected 2550708 READS because READLEN < 1
Read 2550708 spots for ERR4018151.sra
Written 2550708 spots for ERR4018151.sra
SRR ids: ['ERR4018151.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd__28m_5d7
ERR4018151.sra spots: 20405664
blocks: [[1, 2550708], [2550709, 5101416], [5101417, 7652124], [7652125, 10202832], [10202833, 12753540], [12753541, 15304248], [15304249, 17854956], [17854957, 20405664]]
ERR4018151 file size 3884076
ERR4018151 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o ERR4018151 ERR4018151_1.fastq
Input file:	ERR4018151_1.fastq
trimmed:	ERR4018151-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Sun Dec 28 15:06:08 2025 >> started

Sun Dec 28 15:06:15 2025 >> done (7.181s)
20405664 reads processed; of these:
       0 ( 0.00%) short reads filtered out after trimming by size control
       0 ( 0.00%) empty reads filtered out after trimming by size control
20405664 (100.00%) reads available; of these:
    3691 ( 0.02%) trimmed reads available after processing
20401973 (99.98%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 42	      62	  0.00%
 43	      73	  0.00%
 44	      54	  0.00%
 45	     416	  0.00%
 46	     474	  0.00%
 47	     555	  0.00%
 48	     407	  0.00%
 49	     502	  0.00%
 50	     115	  0.00%
 51	     220	  0.00%
 52	     347	  0.00%
 53	      29	  0.00%
 54	     156	  0.00%
 55	      16	  0.00%
 56	       4	  0.00%
 57	      11	  0.00%
 58	       1	  0.00%
 59	       0	  0.00%
 60	       0	  0.00%
 61	       0	  0.00%
 62	       0	  0.00%
 63	       6	  0.00%
 64	       0	  0.00%
 65	       0	  0.00%
 66	       0	  0.00%
 67	      18	  0.00%
 68	       8	  0.00%
 69	      79	  0.00%
 70	       9	  0.00%
 71	       6	  0.00%
 72	       5	  0.00%
 73	      62	  0.00%
 74	       7	  0.00%
 75	      49	  0.00%
 76	20401973	 99.98%
20405664 reads passed initial QC


criterion=sequence-density
sequence-density=96.17
sequence-density-rank=1
fanout-score=25.99
fanout-score-rank=1
prefix-density=96.99
prefix-fanout=25.8
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGCCGTCTTCTGCTTGAAAAA


criterion=fanout-score
sequence-density=96.17
sequence-density-rank=1
fanout-score=25.99
fanout-score-rank=1
prefix-density=96.99
prefix-fanout=25.8
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGCCGTCTTCTGCTTGAAAAA
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGCCGTCTTCTGCTTGAAAAA -o ERR4018151 -
Input file:	STDIN
trimmed:	ERR4018151-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGCCGTCTTCTGCTTGA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Sun Dec 28 15:06:34 2025 >> started

Sun Dec 28 15:06:50 2025 >> done (16.111s)
19984929 reads processed; of these:
 3766993 (18.85%) short reads filtered out after trimming by size control
     381 ( 0.00%) empty reads filtered out after trimming by size control
16217555 (81.15%) reads available; of these:
15942430 (98.30%) trimmed reads available after processing
  275125 ( 1.70%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  766953	  4.73%
 19	  870928	  5.37%
 20	  980361	  6.05%
 21	 2046325	 12.62%
 22	 2577514	 15.89%
 23	 1545890	  9.53%
 24	  776222	  4.79%
 25	  461213	  2.84%
 26	  260592	  1.61%
 27	  263738	  1.63%
 28	  361663	  2.23%
 29	  322644	  1.99%
 30	  648796	  4.00%
 31	 1757662	 10.84%
 32	  534166	  3.29%
 33	  259297	  1.60%
 34	  366506	  2.26%
 35	  214812	  1.32%
 36	   76443	  0.47%
 37	   60390	  0.37%
 38	   51475	  0.32%
 39	   46947	  0.29%
 40	   31994	  0.20%
 41	   37036	  0.23%
 42	   42829	  0.26%
 43	   34969	  0.22%
 44	   41341	  0.25%
 45	   28270	  0.17%
 46	   27748	  0.17%
 47	   44277	  0.27%
 48	   30366	  0.19%
 49	   87219	  0.54%
 50	   57382	  0.35%
 51	   15588	  0.10%
 52	    8902	  0.05%
 53	    8589	  0.05%
 54	    7526	  0.05%
 55	    6950	  0.04%
 56	    7904	  0.05%
 57	   18926	  0.12%
 58	   16814	  0.10%
 59	   11629	  0.07%
 60	    9576	  0.06%
 61	   13608	  0.08%
 62	    7926	  0.05%
 63	    7346	  0.05%
 64	    8211	  0.05%
 65	    5756	  0.04%
 66	   13448	  0.08%
 67	    9326	  0.06%
 68	   10709	  0.07%
 69	    4844	  0.03%
 70	    6288	  0.04%
 71	   16101	  0.10%
 72	    6661	  0.04%
 73	    6006	  0.04%
 74	       1	  0.00%
 75	      24	  0.00%
 76	  274928	  1.70%


criterion=sequence-density
sequence-density=7.03
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=8
prefix-density=0.00
prefix-fanout=1.0
sequence=GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCA


criterion=fanout-score
sequence-density=0.36
sequence-density-rank=21
fanout-score=3.81
fanout-score-rank=1
prefix-density=1.38
prefix-fanout=1.0
sequence=TAGTAGGTTGTGTGGTTT
                                 Started job on |	Dec 28 15:07:00
                             Started mapping on |	Dec 28 15:07:00
                                    Finished on |	Dec 28 15:07:51
       Mapping speed, Million of reads per hour |	1174.47

                          Number of input reads |	16638290
                      Average input read length |	27
                                    UNIQUE READS:
                   Uniquely mapped reads number |	3744801
                        Uniquely mapped reads % |	22.51%
                          Average mapped length |	25.29
                       Number of splices: Total |	15016
            Number of splices: Annotated (sjdb) |	6460
                       Number of splices: GT/AG |	12837
                       Number of splices: GC/AG |	162
                       Number of splices: AT/AC |	22
               Number of splices: Non-canonical |	1995
                      Mismatch rate per base, % |	0.23%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.05
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.61
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	7469578
             % of reads mapped to multiple loci |	44.89%
        Number of reads mapped to too many loci |	4678400
             % of reads mapped to too many loci |	28.12%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	4.09%
                     % of reads unmapped: other |	0.39%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	5423911	5423911	5423911
N_multimapping	7469578	7469578	7469578
N_noFeature	198721	317350	3619963
N_ambiguous	110472	104259	18
UnstrandedReadsAssigned:3435608 PositiveStrandReadsAssigned:3323192 NegativeStrandReadsAssigned:124820
Dataset is classified positive stranded
MeadianReadLen=23 20thPercentileLength=21 echo kmer=19
ERR4018151 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 31,715
[index] number of k-mers: 49,362,984
[index] number of equivalence classes: 166,181
[quant] running in single-end mode
[quant] will process file 1: ERR4018151-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 16,638,290 reads, 2,343,478 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 723 rounds

  31716 ERR4018151.ke.tsv
  32883 ERR4018151.se.tsv
  64599 total
==> ERR4018151.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	856	184	40.9415
ENSRNOT00000040993.4	1039	940	208	42.1459
ENSRNOT00000050156.3	1545	1446	2346	309.015
ENSRNOT00000043693.3	684	585	805	262.096
ENSRNOT00000046201.3	204	105	90	163.258
ENSRNOT00000046108.3	681	582	258	84.4339
ENSRNOT00000049683.3	784	685	360	100.1
ENSRNOT00000041241.3	348	249	78	59.6644
ENSRNOT00000044582.3	297	198	91	87.538

==> ERR4018151.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	0
ENSRNOG00000058808	0
ENSRNOG00000061316	2
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	0
ERR4018151 completed mapping pipeline successfully
