Starting /dee2/code/volunteer_pipeline.sh ERR4018143
    current disk space = 379916468
    free memory = 195958500 
ERR4018143 SRAfilesize
3ec8866f990eda1472eea96238fe0bae  ERR4018143.sra
ERR4018143.sra file validated
ERR4018143 is single end
ERR4018143 is conventional basespace
ERR4018143 read1 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR4018143_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1685	35.0	35.0	35.0	15.0	35.0
2	33.80475	35.0	35.0	35.0	27.0	35.0
3	34.19475	35.0	35.0	35.0	32.0	35.0
4	34.4665	35.0	35.0	35.0	33.0	35.0
5	33.753	35.0	35.0	35.0	31.0	35.0
6	38.47575	39.0	39.0	40.0	36.0	40.0
7	38.97	40.0	39.0	40.0	38.0	40.0
8	39.12475	40.0	39.0	40.0	38.0	40.0
9	38.9745	40.0	39.0	40.0	37.0	40.0
10	39.19175	40.0	39.0	40.0	38.0	40.0
11	39.0335	40.0	39.0	40.0	38.0	40.0
12	39.125	40.0	39.0	40.0	38.0	40.0
13	39.0435	40.0	39.0	40.0	38.0	40.0
14	39.0595	40.0	39.0	40.0	38.0	40.0
15	39.0515	40.0	39.0	40.0	38.0	40.0
16	39.0975	40.0	39.0	40.0	38.0	40.0
17	39.1925	40.0	39.0	40.0	38.0	40.0
18	39.08525	40.0	39.0	40.0	38.0	40.0
19	39.13775	40.0	39.0	40.0	38.0	40.0
20	39.13825	40.0	39.0	40.0	38.0	40.0
21	39.11075	40.0	39.0	40.0	38.0	40.0
22	39.12475	40.0	39.0	40.0	38.0	40.0
23	39.09675	40.0	39.0	40.0	38.0	40.0
24	39.115	40.0	39.0	40.0	38.0	40.0
25	39.11325	40.0	39.0	40.0	38.0	40.0
26	39.06825	40.0	39.0	40.0	38.0	40.0
27	39.14075	40.0	39.0	40.0	38.0	40.0
28	39.18825	40.0	39.0	40.0	38.0	40.0
29	39.193	40.0	39.0	40.0	38.0	40.0
30	39.14275	40.0	39.0	40.0	38.0	40.0
31	39.07075	40.0	39.0	40.0	38.0	40.0
32	39.026	40.0	39.0	40.0	38.0	40.0
33	39.06375	40.0	39.0	40.0	38.0	40.0
34	39.03975	40.0	39.0	40.0	38.0	40.0
35	39.029	40.0	39.0	40.0	38.0	40.0
36	39.01175	40.0	39.0	40.0	38.0	40.0
37	38.92975	40.0	39.0	40.0	38.0	40.0
38	38.938	40.0	39.0	40.0	38.0	40.0
39	38.8595	40.0	39.0	40.0	37.0	40.0
40	38.9595	40.0	39.0	40.0	38.0	40.0
41	38.966	40.0	39.0	40.0	38.0	40.0
42	38.92125	40.0	39.0	40.0	38.0	40.0
43	38.94975	40.0	39.0	40.0	38.0	40.0
44	38.98675	40.0	39.0	40.0	38.0	40.0
45	38.93475	40.0	39.0	40.0	37.0	40.0
46	38.928	40.0	39.0	40.0	38.0	40.0
47	38.90725	40.0	39.0	40.0	38.0	40.0
48	38.9305	40.0	39.0	40.0	38.0	40.0
49	38.621	40.0	39.0	40.0	36.0	40.0
50	38.67325	40.0	39.0	40.0	37.0	40.0
51	38.36125	39.0	39.0	40.0	36.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	0.0
21	0.0
22	0.0
23	0.0
24	1.0
25	1.0
26	3.0
27	5.0
28	9.0
29	8.0
30	15.0
31	24.0
32	31.0
33	33.0
34	47.0
35	80.0
36	126.0
37	297.0
38	1199.0
39	2119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.202053844018874	18.123785734110463	36.55287260616153	21.12128781570913
2	41.725	14.95	33.2	10.125
3	22.75	34.75	13.175	29.325000000000003
4	16.6	38.7	19.2	25.5
5	20.4	41.65	15.25	22.7
6	23.825	19.900000000000002	46.35	9.925
7	55.7	7.2749999999999995	28.749999999999996	8.275
8	29.025000000000002	48.65	12.25	10.075000000000001
9	11.15	37.7	42.675000000000004	8.475000000000001
10	51.775000000000006	13.125	26.724999999999998	8.375
11	41.75	41.4	9.925	6.925000000000001
12	27.425	28.375	19.15	25.05
13	16.325	32.625	20.825	30.225
14	10.8	39.025	34.0	16.175
15	16.875	21.099999999999998	35.375	26.650000000000002
16	13.575000000000001	31.95	34.1	20.375
17	21.725	23.599999999999998	18.375	36.3
18	17.549999999999997	32.275	30.8	19.375
19	15.425	31.0	33.300000000000004	20.275000000000002
20	16.2	23.7	34.925	25.174999999999997
21	23.375	15.575	52.425	8.625
22	29.5	23.35	30.55	16.6
23	26.650000000000002	30.9	11.975	30.475
24	31.674999999999997	26.924999999999997	20.599999999999998	20.8
25	30.975	24.375	15.174999999999999	29.475
26	26.474999999999998	21.85	23.025000000000002	28.65
27	30.425	21.875	13.375	34.325
28	18.224999999999998	20.599999999999998	27.525	33.650000000000006
29	28.299999999999997	17.925	29.675	24.099999999999998
30	35.3	17.675	26.924999999999997	20.1
31	27.875	27.800000000000004	24.775	19.55
32	26.125	23.775	22.175	27.925
33	14.274999999999999	29.45	20.5	35.775
34	14.575	17.825	30.75	36.85
35	25.35	23.799999999999997	25.424999999999997	25.424999999999997
36	31.35	39.375	10.05	19.225
37	12.725	44.925	22.775000000000002	19.575
38	21.6	33.425	19.15	25.825
39	24.7	22.625	27.950000000000003	24.725
40	30.4	16.5	22.125	30.975
41	32.35	26.525	23.125	18.0
42	20.225	38.125	29.675	11.975
43	21.125	29.95	24.0	24.925
44	25.85	36.0	22.650000000000002	15.5
45	23.849999999999998	40.35	18.9	16.900000000000002
46	22.7	33.85	22.95	20.5
47	14.224999999999998	30.099999999999998	37.325	18.35
48	22.325	37.775	21.775	18.125
49	11.325000000000001	36.25	34.050000000000004	18.375
50	24.575	19.75	21.525	34.150000000000006
51	24.775	22.425	24.5	28.299999999999997
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	1.0
21	0.5
22	0.0
23	1.0
24	2.0
25	1.5
26	2.0
27	3.0
28	18.0
29	33.0
30	44.5
31	56.0
32	42.0
33	28.0
34	63.5
35	99.0
36	305.5
37	512.0
38	465.5
39	419.0
40	426.0
41	433.0
42	278.0
43	123.0
44	137.5
45	152.0
46	249.0
47	346.0
48	297.0
49	248.0
50	408.5
51	569.0
52	468.0
53	367.0
54	290.0
55	213.0
56	170.5
57	128.0
58	133.5
59	139.0
60	90.0
61	41.0
62	28.0
63	15.0
64	12.0
65	9.0
66	11.5
67	14.0
68	20.0
69	26.0
70	22.0
71	18.0
72	9.5
73	1.0
74	2.0
75	1.5
76	0.0
77	0.5
78	1.0
79	0.5
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	9.925
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.92152627789777	28.1
2	7.199424046076314	5.0
3	2.3038156947444204	2.4
4	2.159827213822894	3.0
5	1.0079193664506838	1.7500000000000002
6	0.8639308855291578	1.7999999999999998
7	0.7199424046076314	1.7500000000000002
8	0.5039596832253419	1.4000000000000001
9	0.3599712023038157	1.125
>10	3.1677465802735782	20.200000000000003
>50	0.3599712023038157	8.625
>100	0.4319654427645789	24.85
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC	232	5.800000000000001	TruSeq Adapter, Index 14 (98% over 50bp)
TGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAAACT	222	5.55	No Hit
GTAAATGATGAGATTTCCATTGGTCCGTGTTTCTGAACTCCATGATTTTTG	156	3.9	No Hit
TGCACTGATGAAACTAGAAATAGGAAGTGCCGTCCGAAACGACAACTGACG	143	3.5749999999999997	No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAAAC	134	3.35	No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCT	107	2.675	TruSeq Adapter, Index 14 (98% over 51bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG	79	1.975	No Hit
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATAAAAC	76	1.9	No Hit
TACTGTGATGATAACATAGTTCAGCAGACGTGACTCTGGTGAACAATCATG	75	1.875	No Hit
ATACATGATGATCTCACTCCAACTTGAACTCTCTCACTGATTACTTGATGA	61	1.525	No Hit
CACAATGATGACTTATGTTTTTGCCGTTTACCCAGCTGAGGGTTTCTTTGA	54	1.35	No Hit
TTGCAATGATGTGAATCTTTTGCTGAAGTGACCTTGAAGTGTAATTCCTGA	46	1.15	No Hit
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCAT	43	1.075	TruSeq Adapter, Index 14 (97% over 41bp)
TGGAATGATGACATTCTCCGGAATCGCTGTACTGTCTTGATGAAAGTACTT	39	0.975	No Hit
GGTCTGTGATGAAACCCATTATCAGTGGACATCTATGGATGAGAAATGCGG	34	0.8500000000000001	No Hit
TGCTGTGATGAGGACACCAGTAGGAAGTGCCGTCAGAGTCGATAACTGACG	32	0.8	No Hit
CTGCGATGATGTCATTTCTTAGGACACCTTTGGATTAATCATGAAAATAGC	27	0.675	No Hit
NGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC	25	0.625	TruSeq Adapter, Index 14 (98% over 50bp)
TTGAGTGATGACTTGTATTGTCGGATACCCCTTCACCCCGTTCATGGGTGA	25	0.625	No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG	24	0.6	No Hit
CTTAATGATGATTGTCTATTTGATTGCTTGATGCAATGTGAAAACCACATT	24	0.6	No Hit
GCTAATGATGATACCGCACTAACTGAGCAGTTAGTAGTTGGTCCTTTGATT	23	0.575	No Hit
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGATGTGGAGAAAGGTA	23	0.575	No Hit
GGTCAATGATGTGTTGGCATGTATTATCTGAATTTGCTGATGTGTCCTAAC	23	0.575	No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTCC	22	0.5499999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
NGCACTGATGAAACTAGAAATAGGAAGTGCCGTCCGAAACGACAACTGACG	21	0.525	No Hit
AGTAAATGATGAGATTTCCATTGGTCCGTGTTTCTGAACTCCATGATTTTT	21	0.525	No Hit
NGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAAACT	20	0.5	No Hit
ATCGATGTGGTGCTGCCGCGTTCTCTTCGGGCCGGGGCCTAAGCCGCGCCA	20	0.5	No Hit
GTTCTGTGATGAGACTCTGGAATGTGCGCTGGGCACAGCGCCCGAGTGAGA	20	0.5	No Hit
TTCTGTGATGAGACTCTGGAATGTGCGCTGGGCACAGCGCCCGAGTGAGAC	16	0.4	No Hit
NTAAATGATGAGATTTCCATTGGTCCGTGTTTCTGAACTCCATGATTTTTG	15	0.375	No Hit
NTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAAAC	15	0.375	No Hit
GTGGAATGATGACATTCTCCGGAATCGCTGTACTGTCTTGATGAAAGTACT	14	0.35000000000000003	No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGGA	14	0.35000000000000003	No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGAGATCGGAAGAGCACACGT	13	0.325	No Hit
CACAAATGATGAATCCTTTGACGGGCGGGCAGAATCTCTGTGCTGATTGTC	13	0.325	No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCCA	12	0.3	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGATGATGACACCTGGGTATGCTGCACATTCCCGACTGCGTCGTGGGGA	12	0.3	No Hit
NTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG	12	0.3	No Hit
CGCGACCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCAT	12	0.3	TruSeq Adapter, Index 14 (97% over 41bp)
AACAGTGATGACCACAACATGCCATCTGAGTTGCTTTGCTGAAATCCAGAG	11	0.27499999999999997	No Hit
TCGATGTGGTGCTGCCGCGTTCTCTTCGGGCCGGGGCCTAAGCCGCGCCAG	11	0.27499999999999997	No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCCA	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTCTGTGATGACTTTACCCAAATGACTTTCGTTCTTCTGAGTTTGCTGAA	11	0.27499999999999997	No Hit
AAATATGATGAGCATACAAAATGTTGATTACAGTGAAAAACAGTAAGATCC	11	0.27499999999999997	No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGAGATCGGAAGAGCACACGTC	11	0.27499999999999997	No Hit
CTGCAGTGATGACTTTCTTAGGACACCTTTGGATTTACCGTGAAAATAAGT	11	0.27499999999999997	No Hit
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACTC	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
NGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCT	10	0.25	TruSeq Adapter, Index 14 (97% over 35bp)
TATCGATGATGTCCTAAAAGTAAATGTCTGAACATATGAATGCTATACTGA	10	0.25	No Hit
CTCCCATGATGTATAGCACTGGGCTCTGACCACCATGGTGACGTGTTGTGC	10	0.25	No Hit
TTTGAGTGATGACTTGTATTGTCGGATACCCCTTCACCCCGTTCATGGGTG	10	0.25	No Hit
NTACATGATGATCTCACTCCAACTTGAACTCTCTCACTGATTACTTGATGA	10	0.25	No Hit
TGCAATGATGAAGGTTATGTTGGTAGGGACAACTGACCTTGTTGATGAATA	10	0.25	No Hit
NTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATAAAAC	9	0.22499999999999998	No Hit
GAAATATGATGAGCATACAAAATGTTGATTACAGTGAAAAACAGTAAGATC	9	0.22499999999999998	No Hit
NTGCAATGATGTGAATCTTTTGCTGAAGTGACCTTGAAGTGTAATTCCTGA	9	0.22499999999999998	No Hit
TTTCTGTGATGAATCAACTAGCTCACTATGACTGCTAATGAAAACACTGGA	9	0.22499999999999998	No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAGT	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCCA	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAAATGATGATATCACCTTAAATAGCTGGAATTACCGGCAGATTGCTAGT	8	0.2	No Hit
CGTTGGATCGATGTGGTGCTGCCGCGTTCTCTTCGGGCCGGGGCCTAAGCC	8	0.2	No Hit
CTTCGGTGATGACACGATGACGAGTCAGAAAGGCCACGTCTTGCTCTTGGG	8	0.2	No Hit
NGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCAT	8	0.2	TruSeq Adapter, Index 14 (97% over 41bp)
TGCAATGATGTGTGAATTTCTTCACCTGAAATCACCGTGAAGAGTAATTTA	8	0.2	No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	8	0.2	No Hit
CTAGACTGAGGCTCCTTGAGGAGATCGGAAGAGCACACGTCTGAACTCCAG	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCAT	7	0.17500000000000002	TruSeq Adapter, Index 14 (97% over 41bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTA	7	0.17500000000000002	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA	7	0.17500000000000002	No Hit
CGACTCTTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCC	7	0.17500000000000002	TruSeq Adapter, Index 14 (100% over 39bp)
TACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATC	7	0.17500000000000002	No Hit
ATACTGTGATGATAACATAGTTCAGCAGACGTGACTCTGGTGAACAATCAT	7	0.17500000000000002	No Hit
CCGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCA	7	0.17500000000000002	TruSeq Adapter, Index 14 (97% over 39bp)
CACCCTGATGAACTGAATACCGCCCCAGTCTGATAGCTGTGGAGAAAGGTA	7	0.17500000000000002	No Hit
CTACAATGATGGTAATATTTTTCGTCAACAGAGTTCACCTAGTGAGTGTTG	7	0.17500000000000002	No Hit
CTTCGGTGATGACACGATGACGAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACAAGTGATGACTAACAAAGGGACTTAATACTGAAACATCTGGGATTGAAA	6	0.15	No Hit
AATACATGATGATCTCACTCCAACTTGAACTCTCTCACTGATTACTTGATG	6	0.15	No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG	6	0.15	No Hit
NAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTCC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAAGCTGATGATACCACTTCTTTCCCCATCAGATCGACCCTGTTGATCTCA	6	0.15	No Hit
TGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATAAAACA	6	0.15	No Hit
TGAAGTGATGATTCACAATCATGTCTCTTCTCTGAAGAATTCTTGGAGATT	6	0.15	No Hit
TGTCCCATTTGACTACCACATTTCCTACTGATAATAGATTTTGGTGGCTAT	6	0.15	No Hit
TGGAGATGATGAACTGACTCAACCTGACCTTCAGAAATGGAGGTGATAAAA	6	0.15	No Hit
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTCC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGCAATGATGTGTGAATTTCTTCACCTGAAATCACCGTGAAGAGTAATTT	5	0.125	No Hit
CCGATACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC	5	0.125	No Hit
ATATATGATGACTTTCATAGAATCTCGTTCGGCTGATGATTGCTGTTGAGA	5	0.125	No Hit
GTGCAATGATGCATTTTATTAATCAACACATCATTCTGAAAGCAGATGTGT	5	0.125	No Hit
ACAAATGATGAATCCTTTGACGGGCGGGCAGAATCTCTGTGCTGATTGTCA	5	0.125	No Hit
ACTTAATGATGATTGTCTATTTGATTGCTTGATGCAATGTGAAAACCACAT	5	0.125	No Hit
GTTAGTGATGATCAATTAAGTTAAAACAGATGGGAATCTCTCTGAACAACA	5	0.125	No Hit
ATCTATGATGATCCTATCCCGAATCTGAATTCCTGTTGAAAAAATATAATT	5	0.125	No Hit
NTGCGATGATGTCATTTCTTAGGACACCTTTGGATTAATCATGAAAATAGC	5	0.125	No Hit
ACCCCCTGGACATGGGCAAGGAGACACCATGTTGGTGGTGTTGTTCCTATA	5	0.125	No Hit
TGAGGTAGTAGGTTGTATGGTTAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCCCTGTGATGTTCAGCACTGGGCTCTGATCACCCTGAGGACATGGAGCT	5	0.125	No Hit
GGTTATGATGAAATTCCACTTCATGGTCCGTGTTTCTGAAATAACATGATT	5	0.125	No Hit
GGAGATGATGAACTGACTCAACCTGACCTTCAGAAATGGAGGTGATAAAAC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.175	0.0	0.0	0.0	0.0
2	6.225	0.0	0.0	0.0	0.0
3	6.275	0.0	0.0	0.0	0.0
4	6.3	0.0	0.0	0.0	0.0
5	6.325	0.0	0.0	0.0	0.0
6	6.425	0.0	0.0	0.0	0.0
7	6.45	0.0	0.0	0.0	0.0
8	6.625	0.0	0.0	0.0	0.0
9	6.8	0.0	0.0	0.0	0.0
10	8.975	0.0	0.0	0.0	0.0
11	9.45	0.0	0.0	0.0	0.0
12	10.175	0.0	0.0	0.0	0.0
13	10.55	0.0	0.0	0.0	0.0
14	11.2	0.0	0.0	0.0	0.0
15	11.675	0.0	0.0	0.0	0.0
16	12.025	0.0	0.0	0.0	0.0
17	12.35	0.0	0.0	0.0	0.0
18	12.8	0.0	0.0	0.0	0.0
19	13.125	0.0	0.0	0.0	0.0
20	13.575	0.0	0.0	0.0	0.0
21	14.325	0.0	0.0	0.0	0.0
22	15.725	0.0	0.0	0.0	0.0
23	19.5	0.0	0.0	0.0	0.0
24	21.8	0.0	0.0	0.0	0.0
25	23.25	0.0	0.0	0.0	0.0
26	23.75	0.0	0.0	0.0	0.0
27	24.225	0.0	0.0	0.0	0.0
28	24.625	0.0	0.0	0.0	0.0
29	25.1	0.0	0.0	0.0	0.0
30	25.4	0.0	0.0	0.0	0.0
31	25.775	0.0	0.0	0.0	0.0
32	26.75	0.0	0.0	0.0	0.0
33	27.65	0.0	0.0	0.0	0.0
34	27.875	0.0	0.0	0.0	0.0
35	28.025	0.0	0.0	0.0	0.0
36	28.225	0.0	0.0	0.0	0.0
37	28.525	0.0	0.0	0.0	0.0
38	28.825	0.0	0.0	0.0	0.0
39	29.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGGTA	15	0.007414995	50.577465	1
CGACAAC	35	8.73988E-8	44.8875	40
TAGAAAT	35	8.73988E-8	44.8875	15
AAACTAG	35	8.73988E-8	44.8875	11
GAAACTA	35	8.73988E-8	44.8875	10
TAGGAAG	30	1.7006078E-6	44.8875	21
GAAATAG	35	8.73988E-8	44.8875	17
ACTGACG	35	8.73988E-8	44.8875	45
AACGACA	35	8.73988E-8	44.8875	38
GAAGTGC	30	1.7006078E-6	44.8875	24
GTCCGAA	35	8.73988E-8	44.8875	32
ATAGGAA	30	1.7006078E-6	44.8875	20
AAATAGG	35	8.73988E-8	44.8875	18
AGGAAGT	30	1.7006078E-6	44.8875	22
ATGAAAC	35	8.73988E-8	44.8875	8
CCGTCCG	35	8.73988E-8	44.8875	30
AGAAATA	35	8.73988E-8	44.8875	16
AATAGGA	30	1.7006078E-6	44.8875	19
AGTGCCG	35	8.73988E-8	44.8875	26
AACTAGA	35	8.73988E-8	44.8875	12
>>END_MODULE
Rejected 2704158 READS because READLEN < 1
Read 2704158 spots for ERR4018143.sra
Written 2704158 spots for ERR4018143.sra
Rejected 2704158 READS because READLEN < 1
Read 2704158 spots for ERR4018143.sra
Written 2704158 spots for ERR4018143.sra
Rejected 2704165 READS because READLEN < 1
Read 2704165 spots for ERR4018143.sra
Written 2704165 spots for ERR4018143.sra
Rejected 2704158 READS because READLEN < 1
Read 2704158 spots for ERR4018143.sra
Written 2704158 spots for ERR4018143.sra
Rejected 2704158 READS because READLEN < 1
Read 2704158 spots for ERR4018143.sra
Written 2704158 spots for ERR4018143.sra
Rejected 2704158 READS because READLEN < 1
Read 2704158 spots for ERR4018143.sra
Written 2704158 spots for ERR4018143.sra
Rejected 2704158 READS because READLEN < 1
Read 2704158 spots for ERR4018143.sra
Written 2704158 spots for ERR4018143.sra
Rejected 2704158 READS because READLEN < 1
Read 2704158 spots for ERR4018143.sra
Written 2704158 spots for ERR4018143.sra
SRR ids: ['ERR4018143.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_x2pa1mcf
ERR4018143.sra spots: 21633271
blocks: [[1, 2704158], [2704159, 5408316], [5408317, 8112474], [8112475, 10816632], [10816633, 13520790], [13520791, 16224948], [16224949, 18929106], [18929107, 21633271]]
ERR4018143 file size 3062732
ERR4018143 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o ERR4018143 ERR4018143_1.fastq
Input file:	ERR4018143_1.fastq
trimmed:	ERR4018143-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Sun Dec 28 14:59:32 2025 >> started

Sun Dec 28 14:59:39 2025 >> done (6.943s)
21633271 reads processed; of these:
 1484422 ( 6.86%) short reads filtered out after trimming by size control
 1528938 ( 7.07%) empty reads filtered out after trimming by size control
18619911 (86.07%) reads available; of these:
  204221 ( 1.10%) trimmed reads available after processing
18415690 (98.90%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  111924	  0.60%
 19	   92184	  0.50%
 20	       0	  0.00%
 21	       0	  0.00%
 22	       0	  0.00%
 23	       0	  0.00%
 24	       0	  0.00%
 25	       0	  0.00%
 26	       0	  0.00%
 27	       0	  0.00%
 28	       0	  0.00%
 29	       0	  0.00%
 30	       0	  0.00%
 31	       0	  0.00%
 32	       0	  0.00%
 33	       0	  0.00%
 34	       0	  0.00%
 35	       0	  0.00%
 36	       0	  0.00%
 37	       0	  0.00%
 38	       0	  0.00%
 39	       0	  0.00%
 40	       0	  0.00%
 41	       0	  0.00%
 42	       0	  0.00%
 43	       0	  0.00%
 44	       0	  0.00%
 45	       0	  0.00%
 46	       0	  0.00%
 47	       0	  0.00%
 48	       0	  0.00%
 49	      49	  0.00%
 50	      64	  0.00%
 51	18415690	 98.90%
18619911 reads passed initial QC


criterion=sequence-density
sequence-density=17.32
sequence-density-rank=1
fanout-score=33.28
fanout-score-rank=1
prefix-density=17.99
prefix-fanout=32.0
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTC


criterion=fanout-score
sequence-density=17.32
sequence-density-rank=1
fanout-score=33.28
fanout-score-rank=1
prefix-density=17.99
prefix-fanout=32.0
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTC -o ERR4018143 -
Input file:	STDIN
trimmed:	ERR4018143-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGCCGTCTTC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Sun Dec 28 14:59:52 2025 >> started

Sun Dec 28 15:00:00 2025 >> done (8.352s)
16551032 reads processed; of these:
    6461 ( 0.04%) short reads filtered out after trimming by size control
    2200 ( 0.01%) empty reads filtered out after trimming by size control
16542371 (99.95%) reads available; of these:
 4314284 (26.08%) trimmed reads available after processing
12228087 (73.92%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	   96571	  0.58%
 19	   83655	  0.51%
 20	  133418	  0.81%
 21	  281357	  1.70%
 22	  689915	  4.17%
 23	  445961	  2.70%
 24	  274875	  1.66%
 25	   92945	  0.56%
 26	   64152	  0.39%
 27	   60607	  0.37%
 28	   75667	  0.46%
 29	   54340	  0.33%
 30	  101460	  0.61%
 31	  162302	  0.98%
 32	  126993	  0.77%
 33	   66728	  0.40%
 34	   58002	  0.35%
 35	   44717	  0.27%
 36	   64760	  0.39%
 37	   57056	  0.34%
 38	   41432	  0.25%
 39	   43517	  0.26%
 40	   29298	  0.18%
 41	   29132	  0.18%
 42	   52628	  0.32%
 43	  100673	  0.61%
 44	  156776	  0.95%
 45	  566883	  3.43%
 46	  118845	  0.72%
 47	   43169	  0.26%
 48	  273128	  1.65%
 49	      32	  0.00%
 50	      40	  0.00%
 51	12051337	 72.85%


criterion=sequence-density
sequence-density=4.84
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=12
prefix-density=0.01
prefix-fanout=1.0
sequence=GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAAACTCTCTGCTGATC


criterion=fanout-score
sequence-density=1.10
sequence-density-rank=5
fanout-score=6.61
fanout-score-rank=1
prefix-density=7.18
prefix-fanout=1.0
sequence=GAAGTGCCGTCAGAGTCGATAACTGACGATA
                                 Started job on |	Dec 28 15:00:09
                             Started mapping on |	Dec 28 15:00:10
                                    Finished on |	Dec 28 15:00:42
       Mapping speed, Million of reads per hour |	2093.77

                          Number of input reads |	18611250
                      Average input read length |	46
                                    UNIQUE READS:
                   Uniquely mapped reads number |	13367921
                        Uniquely mapped reads % |	71.83%
                          Average mapped length |	47.49
                       Number of splices: Total |	10748
            Number of splices: Annotated (sjdb) |	2765
                       Number of splices: GT/AG |	9993
                       Number of splices: GC/AG |	155
                       Number of splices: AT/AC |	3
               Number of splices: Non-canonical |	597
                      Mismatch rate per base, % |	0.37%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.08
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.20
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	3443034
             % of reads mapped to multiple loci |	18.50%
        Number of reads mapped to too many loci |	327155
             % of reads mapped to too many loci |	1.76%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	7.82%
                     % of reads unmapped: other |	0.09%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1800295	1800295	1800295
N_multimapping	3443034	3443034	3443034
N_noFeature	372987	491604	12796618
N_ambiguous	1213990	761263	43
UnstrandedReadsAssigned:11780944 PositiveStrandReadsAssigned:12115054 NegativeStrandReadsAssigned:571260
Dataset is classified positive stranded
MeadianReadLen=51 20thPercentileLength=43 echo kmer=39
ERR4018143 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 31,715
[index] number of k-mers: 49,915,185
[index] number of equivalence classes: 70,351
[quant] running in single-end mode
[quant] will process file 1: ERR4018143-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 18,611,250 reads, 129,689 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 574 rounds

  31716 ERR4018143.ke.tsv
  32883 ERR4018143.se.tsv
  64599 total
==> ERR4018143.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	856	85	535.4
ENSRNOT00000040993.4	1039	940	138	791.561
ENSRNOT00000050156.3	1545	1446	886	3303.68
ENSRNOT00000043693.3	684	585	237	2184.37
ENSRNOT00000046201.3	204	105	139	7137.7
ENSRNOT00000046108.3	681	582	46	426.155
ENSRNOT00000049683.3	784	685	83	653.312
ENSRNOT00000041241.3	348	249	37	801.19
ENSRNOT00000044582.3	297	198	66	1797.26

==> ERR4018143.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	0
ENSRNOG00000058808	0
ENSRNOG00000061316	0
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	0
ERR4018143 completed mapping pipeline successfully
