Starting /dee2/code/volunteer_pipeline.sh ERR4018153
    current disk space = 332567772
    free memory = 196117452 
ERR4018153 SRAfilesize
6d7bb3c5da895b369dcfd71016925b67  ERR4018153.sra
ERR4018153.sra file validated
ERR4018153 is single end
ERR4018153 is conventional basespace
ERR4018153 read1 length is 76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR4018153_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	76
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3855	32.0	32.0	32.0	32.0	32.0
2	31.362	32.0	32.0	32.0	32.0	32.0
3	31.47825	32.0	32.0	32.0	32.0	32.0
4	31.52875	32.0	32.0	32.0	32.0	32.0
5	31.62125	32.0	32.0	32.0	32.0	32.0
6	34.98425	36.0	36.0	36.0	36.0	36.0
7	35.182	36.0	36.0	36.0	36.0	36.0
8	35.08625	36.0	36.0	36.0	36.0	36.0
9	35.22425	36.0	36.0	36.0	36.0	36.0
10-11	35.28775	36.0	36.0	36.0	36.0	36.0
12-13	35.193625	36.0	36.0	36.0	36.0	36.0
14-15	35.143375000000006	36.0	36.0	36.0	36.0	36.0
16-17	35.24575	36.0	36.0	36.0	36.0	36.0
18-19	35.200374999999994	36.0	36.0	36.0	36.0	36.0
20-21	35.123374999999996	36.0	36.0	36.0	36.0	36.0
22-23	34.98625	36.0	36.0	36.0	36.0	36.0
24-25	34.980125	36.0	36.0	36.0	36.0	36.0
26-27	34.976625	36.0	36.0	36.0	36.0	36.0
28-29	34.975	36.0	36.0	36.0	36.0	36.0
30-31	35.006625	36.0	36.0	36.0	36.0	36.0
32-33	35.089625	36.0	36.0	36.0	36.0	36.0
34-35	35.124625	36.0	36.0	36.0	36.0	36.0
36-37	34.965875	36.0	36.0	36.0	36.0	36.0
38-39	34.954125	36.0	36.0	36.0	36.0	36.0
40-41	34.7585	36.0	36.0	36.0	32.0	36.0
42-43	34.873374999999996	36.0	36.0	36.0	36.0	36.0
44-45	35.01625	36.0	36.0	36.0	36.0	36.0
46-47	34.824375	36.0	36.0	36.0	36.0	36.0
48-49	34.450625	36.0	36.0	36.0	32.0	36.0
50-51	34.067625	36.0	36.0	36.0	32.0	36.0
52-53	33.619749999999996	36.0	36.0	36.0	27.0	36.0
54-55	33.285875000000004	36.0	36.0	36.0	24.0	36.0
56-57	33.04	36.0	34.0	36.0	21.0	36.0
58-59	33.122625	36.0	32.0	36.0	27.0	36.0
60-61	33.073375	36.0	34.0	36.0	24.0	36.0
62-63	32.543625	36.0	32.0	36.0	21.0	36.0
64-65	32.721125	36.0	32.0	36.0	21.0	36.0
66-67	33.130875	36.0	36.0	36.0	24.0	36.0
68-69	33.08125	36.0	36.0	36.0	24.0	36.0
70-71	33.025875	36.0	36.0	36.0	21.0	36.0
72-73	33.030125	36.0	32.0	36.0	20.5	36.0
74-75	33.221999999999994	36.0	36.0	36.0	27.0	36.0
76	31.6385	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	0.0
19	1.0
20	2.0
21	2.0
22	2.0
23	6.0
24	6.0
25	16.0
26	25.0
27	53.0
28	56.0
29	85.0
30	100.0
31	145.0
32	232.0
33	376.0
34	967.0
35	1922.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.2	24.975	15.925	20.9
2	44.85	21.025	12.225	21.9
3	37.525	26.05	12.075	24.349999999999998
4	44.125	18.2	21.025	16.650000000000002
5	37.6	21.725	19.35	21.325
6	32.71263210870659	17.58933064921993	26.37141419224962	23.326623049823855
7	37.7	21.6	23.575	17.125
8	30.075000000000003	16.7	39.975	13.25
9	35.8	26.674999999999997	17.299999999999997	20.225
10-11	43.25	18.1875	18.7	19.8625
12-13	36.1875	17.349999999999998	27.200000000000003	19.2625
14-15	39.1	27.1625	19.15	14.5875
16-17	32.0375	23.275000000000002	28.762500000000003	15.925
18-19	36.162499999999994	18.025	26.4625	19.35
20-21	31.8125	21.625	29.4875	17.075000000000003
22-23	33.0625	21.475	26.237500000000004	19.225
24-25	37.5875	17.849999999999998	24.087500000000002	20.474999999999998
26-27	26.940867608451057	18.952369046130766	26.690836354544317	27.415926990873857
28-29	31.861396047035278	16.800100075056292	26.82011508631474	24.518388791593697
30-31	32.391548943617956	16.889611201400175	23.67795974496812	27.040880110013752
32-33	35.7625	19.85	23.4375	20.95
34-35	35.2625	21.5	21.6875	21.55
36-37	28.599999999999998	24.887500000000003	23.8375	22.675
38-39	27.450000000000003	23.125	21.525	27.900000000000002
40-41	27.237499999999997	27.962500000000002	18.3125	26.487500000000004
42-43	34.7625	25.924999999999997	16.0875	23.225
44-45	29.9625	24.637500000000003	17.8	27.6
46-47	28.6375	21.3	17.2	32.8625
48-49	26.625	24.8625	15.8875	32.625
50-51	28.6875	24.95	17.849999999999998	28.512500000000003
52-53	25.9875	25.8625	19.037499999999998	29.1125
54-55	24.3125	22.6875	21.375	31.624999999999996
56-57	25.087500000000002	17.825	24.05	33.037499999999994
58-59	28.6875	18.575	21.875	30.862499999999997
60-61	24.85	18.6125	22.912499999999998	33.625
62-63	23.125	18.325	26.387500000000003	32.1625
64-65	23.2875	14.5375	28.462500000000002	33.7125
66-67	27.0	13.1875	29.1625	30.65
68-69	24.55	13.6375	31.4	30.412499999999998
70-71	23.65	14.649999999999999	31.0	30.7
72-73	20.6875	13.450000000000001	32.0625	33.800000000000004
74-75	21.8875	14.975	33.625	29.512500000000003
76	20.1	18.25	34.050000000000004	27.6
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.5
24	0.5
25	0.0
26	0.0
27	0.5
28	2.0
29	3.0
30	2.5
31	1.0
32	1.5
33	3.0
34	4.5
35	4.5
36	3.0
37	3.0
38	3.0
39	4.5
40	5.5
41	7.0
42	9.0
43	10.0
44	10.0
45	11.5
46	14.0
47	23.0
48	38.0
49	68.0
50	92.0
51	150.5
52	294.0
53	432.0
54	485.0
55	484.5
56	556.5
57	624.5
58	620.0
59	568.0
60	361.0
61	177.5
62	149.0
63	91.0
64	29.0
65	12.5
66	1.5
67	3.0
68	2.5
69	1.5
70	0.5
71	0.0
72	0.5
73	1.0
74	0.5
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.5
82	0.5
83	0.0
84	0.5
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.5
91	0.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.65
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0125
28-29	0.075
30-31	0.0125
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.75
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.04428044280442	59.650000000000006
2	5.830258302583026	7.9
3	2.3247232472324724	4.725
4	0.8856088560885609	2.4
5	0.5166051660516605	1.7500000000000002
6	0.5535055350553505	2.25
7	0.25830258302583026	1.225
8	0.18450184501845018	1.0
9	0.11070110701107011	0.675
>10	1.2177121771217712	14.649999999999999
>50	0.07380073800738007	3.775
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC	77	1.925	No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	74	1.8499999999999999	No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCATGTGGAATTCTCGGGTGC	37	0.9249999999999999	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA	32	0.8	No Hit
ACAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	32	0.8	RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	32	0.8	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG	28	0.7000000000000001	No Hit
GGAACGGGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGG	25	0.625	RNA PCR Primer, Index 17 (97% over 37bp)
GGGGCTGTAGCTCAGCTGGGAGAGCACCTGCTTTGGAATTCTCGGGTGCC	22	0.5499999999999999	No Hit
AGGGGAAGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCC	22	0.5499999999999999	RNA PCR Primer, Index 20 (100% over 39bp)
CGCGACCTCAGATCAGACGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	21	0.525	RNA PCR Primer, Index 1 (100% over 30bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG	21	0.525	No Hit
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	21	0.525	RNA PCR Primer, Index 1 (100% over 28bp)
GGGTGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCA	18	0.44999999999999996	RNA PCR Primer, Index 20 (100% over 40bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA	18	0.44999999999999996	No Hit
AGGAGGAGAAGGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	17	0.42500000000000004	RNA PCR Primer, Index 6 (100% over 34bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAG	16	0.4	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA	15	0.375	RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	13	0.325	No Hit
AGGAGGAGAAGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT	13	0.325	RNA PCR Primer, Index 1 (97% over 35bp)
CGCGACCTCAGATCAGACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	13	0.325	RNA PCR Primer, Index 1 (100% over 31bp)
GGGAACGGGCTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG	13	0.325	RNA PCR Primer, Index 15 (97% over 36bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA	13	0.325	No Hit
AGGGGAAGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCA	13	0.325	RNA PCR Primer, Index 20 (100% over 40bp)
ACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAG	13	0.325	No Hit
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA	12	0.3	RNA PCR Primer, Index 1 (100% over 23bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA	12	0.3	No Hit
GGATCAAGCGACTAAGTGCATATGGTGGATGCCTTGGCGATCACAGGCGA	11	0.27499999999999997	No Hit
CGCGACCTCAGATCAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 32bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	10	0.25	No Hit
CGCGACCTCAGATCAGACGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
GGGAGGTTGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGC	10	0.25	RNA PCR Primer, Index 20 (100% over 38bp)
GGGGCTGTAGCTCAGCTGGGAGAGCACCTGGAATTCTCGGGTGCCAAGGA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCTGTAGCTCAGCTGGGAGAGCTGGAATTCTCGGGTGCCAAGGAACT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 25bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGTGGAATTCTCGGGTGCCAAG	9	0.22499999999999998	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	8	0.2	No Hit
CAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	8	0.2	RNA PCR Primer, Index 1 (100% over 30bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCATGG	8	0.2	No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCTGGAATTCTCGGGTGCCAA	8	0.2	No Hit
GACTCTTAGCGGTGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	8	0.2	RNA PCR Primer, Index 1 (100% over 33bp)
AGGAGGGGAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGC	7	0.17500000000000002	RNA PCR Primer, Index 20 (100% over 38bp)
GCGGGTGTAGCTCAGGGGTAGAGCTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TGGAGTGTGACAATGGTGTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG	7	0.17500000000000002	No Hit
AGCTACATTGTCTGCTGGGTTTCTGGAATTCTCGGGTGCCAAGGAACTCC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGAACTGAATTCCATGGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCATG	6	0.15	No Hit
GGGGCTATAGCTCAGCTGGGAGAGCTGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
AGGAGGGGAAGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGG	6	0.15	RNA PCR Primer, Index 17 (97% over 37bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCTGGAATTCTCGGGTGC	6	0.15	No Hit
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
GGGGCTATAGCTCAGCTGGGAGAGCGTGGAATTCTCGGGTGCCAAGGAAC	6	0.15	RNA PCR Primer, Index 1 (100% over 24bp)
AGGAGGGGAAGGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT	6	0.15	RNA PCR Primer, Index 1 (97% over 35bp)
AGGGGGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCAT	6	0.15	RNA PCR Primer, Index 20 (100% over 41bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC	6	0.15	No Hit
GCGGGAGAGTAGGTCGCTGCCAGGTCTTGGAATTCTCGGGTGCCAAGGAA	6	0.15	RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT	6	0.15	No Hit
AGCGAACGCAGGGAACTGAAACTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC	5	0.125	No Hit
GACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	5	0.125	RNA PCR Primer, Index 1 (100% over 32bp)
GCGGTCGTGGCGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG	5	0.125	RNA PCR Primer, Index 15 (97% over 36bp)
ACGCGGGAGAGTAGGTCGCTGCCAGGTCTTGGAATTCTCGGGTGCCAAGG	5	0.125	Illumina Small RNA Adapter 2 (100% over 21bp)
AACGGATTTTTGGAGCAGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT	5	0.125	RNA PCR Primer, Index 1 (97% over 35bp)
TGAGGTAGTAGGTTGTGTGGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
AGGAGGAGAAGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG	5	0.125	RNA PCR Primer, Index 15 (97% over 36bp)
GACTCTTAGCGGTGGATCACTCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCTGGAATTCTCGGGTG	5	0.125	No Hit
GGGCGTGTAGCTCAGCGGGAGAGCACTACGTTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGTGGAATTCTCGGGTGCCA	5	0.125	No Hit
CGGAGTGTAGCGCAGTCTGGTAGCGCTGGAATTCTCGGGTGCCAAGGAAC	5	0.125	RNA PCR Primer, Index 1 (100% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.05	0.0	0.0	0.0
3	0.0	0.05	0.0	0.0	0.0
4	0.0	0.05	0.0	0.0	0.0
5	0.0	0.075	0.0	0.0	0.0
6	0.0	0.1	0.0	0.0	0.0
7	0.0	0.5	0.0	0.0	0.0
8	0.0	1.025	0.0	0.0	0.0
9	0.0	1.875	0.0	0.0	0.0
10	0.0	3.6	0.0	0.0	0.0
11	0.0	5.6	0.0	0.0	0.0
12	0.0	7.7	0.0	0.0	0.0
13	0.0	9.8	0.0	0.0	0.0
14	0.0	12.5	0.0	0.0	0.0
15	0.0	15.375	0.0	0.0	0.0
16	0.0	17.7	0.0	0.0	0.0
17	0.0	19.8	0.0	0.0	0.0
18	0.0	22.075	0.0	0.0	0.0
19	0.0	25.05	0.0	0.0	0.0
20	0.0	27.475	0.0	0.0	0.0
21	0.0	30.85	0.0	0.0	0.0
22	0.0	34.8	0.0	0.0	0.0
23	0.0	39.925	0.0	0.0	0.0
24	0.0	43.9	0.0	0.0	0.0
25	0.0	46.45	0.0	0.0	0.0
26	0.0	48.925	0.0	0.0	0.0
27	0.0	51.15	0.0	0.0	0.0
28	0.0	53.8	0.0	0.0	0.0
29	0.0	56.025	0.0	0.0	0.0
30	0.0	58.45	0.0	0.0	0.0
31	0.0	62.725	0.0	0.0	0.0
32	0.0	67.4	0.0	0.0	0.0
33	0.0	69.9	0.0	0.0	0.0
34	0.0	72.4	0.0	0.0	0.0
35	0.0	75.025	0.0	0.0	0.0
36	0.0	76.075	0.0	0.0	0.0
37	0.0	77.2	0.0	0.0	0.0
38	0.0	78.125	0.0	0.0	0.0
39	0.0	78.975	0.0	0.0	0.0
40	0.0	79.925	0.0	0.0	0.0
41	0.0	80.425	0.0	0.0	0.0
42	0.0	81.075	0.0	0.0	0.0
43	0.0	81.475	0.0	0.0	0.0
44	0.0	81.95	0.0	0.0	0.0
45	0.0	82.625	0.0	0.0	0.0
46	0.0	83.175	0.0	0.0	0.0
47	0.0	83.8	0.0	0.0	0.0
48	0.0	84.45	0.0	0.0	0.0
49	0.0	85.075	0.0	0.0	0.0
50	0.0	86.0	0.0	0.0	0.0
51	0.0	86.95	0.0	0.0	0.0
52	0.0	87.5	0.0	0.0	0.0
53	0.0	87.7	0.0	0.0	0.0
54	0.0	88.0	0.0	0.0	0.0
55	0.0	88.325	0.0	0.0	0.0
56	0.0	88.625	0.0	0.0	0.0
57	0.0	89.1	0.0	0.0	0.0
58	0.0	89.45	0.0	0.0	0.0
59	0.0	89.625	0.0	0.0	0.0
60	0.0	89.925	0.0	0.0	0.0
61	0.0	90.1	0.0	0.0	0.0
62	0.0	90.4	0.0	0.0	0.0
63	0.0	90.65	0.0	0.0	0.0
64	0.0	91.05	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGAGAGC	15	0.0020658788	70.00001	19
AGCTCAG	15	0.0020658788	70.00001	9
CTGGGAG	15	0.0020658788	70.00001	16
GCTGGGA	15	0.0020658788	70.00001	15
TAGCTCA	15	0.0020658788	70.00001	8
TCAGCTG	15	0.0020658788	70.00001	12
CTCAGCT	15	0.0020658788	70.00001	11
TGGGAGA	15	0.0020658788	70.00001	17
GCTCAGC	15	0.0020658788	70.00001	10
GGGAGAG	20	0.0064587677	52.5	18
AGCTGGG	20	0.0064587677	52.5	14
TAGCGGT	45	0.003819767	31.111113	7
GCGGTGG	45	0.003819767	31.111113	9
CTCTTAG	45	0.003819767	31.111113	3
ACTCTTA	45	0.003819767	31.111113	2
CTTAGCG	45	0.003819767	31.111113	5
AGCGGTG	45	0.003819767	31.111113	8
CGGTGGA	45	0.003819767	31.111113	10
GTGGATC	45	0.003819767	31.111113	12
GACTCTT	45	0.003819767	31.111113	1
>>END_MODULE
Rejected 3124064 READS because READLEN < 1
Read 3124064 spots for ERR4018153.sra
Written 3124064 spots for ERR4018153.sra
Rejected 3124068 READS because READLEN < 1
Read 3124068 spots for ERR4018153.sra
Written 3124068 spots for ERR4018153.sra
Rejected 3124064 READS because READLEN < 1
Read 3124064 spots for ERR4018153.sra
Written 3124064 spots for ERR4018153.sra
Rejected 3124064 READS because READLEN < 1
Read 3124064 spots for ERR4018153.sra
Written 3124064 spots for ERR4018153.sra
Rejected 3124064 READS because READLEN < 1
Read 3124064 spots for ERR4018153.sra
Written 3124064 spots for ERR4018153.sra
Rejected 3124064 READS because READLEN < 1
Read 3124064 spots for ERR4018153.sra
Written 3124064 spots for ERR4018153.sra
Rejected 3124064 READS because READLEN < 1
Read 3124064 spots for ERR4018153.sra
Written 3124064 spots for ERR4018153.sra
Rejected 3124064 READS because READLEN < 1
Read 3124064 spots for ERR4018153.sra
Written 3124064 spots for ERR4018153.sra
SRR ids: ['ERR4018153.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_wmxl638g
ERR4018153.sra spots: 24992516
blocks: [[1, 3124064], [3124065, 6248128], [6248129, 9372192], [9372193, 12496256], [12496257, 15620320], [15620321, 18744384], [18744385, 21868448], [21868449, 24992516]]
ERR4018153 file size 4762028
ERR4018153 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o ERR4018153 ERR4018153_1.fastq
Input file:	ERR4018153_1.fastq
trimmed:	ERR4018153-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Sun Dec 28 15:11:59 2025 >> started

Sun Dec 28 15:12:07 2025 >> done (7.776s)
24992516 reads processed; of these:
       0 ( 0.00%) short reads filtered out after trimming by size control
       0 ( 0.00%) empty reads filtered out after trimming by size control
24992516 (100.00%) reads available; of these:
    4696 ( 0.02%) trimmed reads available after processing
24987820 (99.98%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 42	      90	  0.00%
 43	     115	  0.00%
 44	      74	  0.00%
 45	     545	  0.00%
 46	     603	  0.00%
 47	     669	  0.00%
 48	     542	  0.00%
 49	     623	  0.00%
 50	     162	  0.00%
 51	     287	  0.00%
 52	     475	  0.00%
 53	      22	  0.00%
 54	     163	  0.00%
 55	      12	  0.00%
 56	       7	  0.00%
 57	      17	  0.00%
 58	       0	  0.00%
 59	       0	  0.00%
 60	       1	  0.00%
 61	       0	  0.00%
 62	       0	  0.00%
 63	       6	  0.00%
 64	       0	  0.00%
 65	       0	  0.00%
 66	       0	  0.00%
 67	      30	  0.00%
 68	      16	  0.00%
 69	      87	  0.00%
 70	      10	  0.00%
 71	       6	  0.00%
 72	       5	  0.00%
 73	      56	  0.00%
 74	      19	  0.00%
 75	      54	  0.00%
 76	24987820	 99.98%
24992516 reads passed initial QC


criterion=sequence-density
sequence-density=91.76
sequence-density-rank=1
fanout-score=39.45
fanout-score-rank=1
prefix-density=93.04
prefix-fanout=38.9
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGTATGCCGTCTTCTGCTTGAAAAA


criterion=fanout-score
sequence-density=91.76
sequence-density-rank=1
fanout-score=39.45
fanout-score-rank=1
prefix-density=93.04
prefix-fanout=38.9
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGTATGCCGTCTTCTGCTTGAAAAA
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGTATGCCGTCTTCTGCTTGAAAAA -o ERR4018153 -
Input file:	STDIN
trimmed:	ERR4018153-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGTATGCCGTCTTCTGCTTGA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Sun Dec 28 15:12:30 2025 >> started

Sun Dec 28 15:12:56 2025 >> done (25.725s)
24449201 reads processed; of these:
 5563572 (22.76%) short reads filtered out after trimming by size control
    3103 ( 0.01%) empty reads filtered out after trimming by size control
18882526 (77.23%) reads available; of these:
17869499 (94.64%) trimmed reads available after processing
 1013027 ( 5.36%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  627948	  3.33%
 19	  709345	  3.76%
 20	  854335	  4.52%
 21	 1160798	  6.15%
 22	 1335169	  7.07%
 23	  961449	  5.09%
 24	  615329	  3.26%
 25	  613128	  3.25%
 26	  585591	  3.10%
 27	  545079	  2.89%
 28	  599866	  3.18%
 29	  578410	  3.06%
 30	  931775	  4.93%
 31	 1209238	  6.40%
 32	  661004	  3.50%
 33	  595657	  3.15%
 34	  704859	  3.73%
 35	  373633	  1.98%
 36	  258544	  1.37%
 37	  242551	  1.28%
 38	  245881	  1.30%
 39	  203048	  1.08%
 40	  164862	  0.87%
 41	  157899	  0.84%
 42	  160919	  0.85%
 43	  138726	  0.73%
 44	  134759	  0.71%
 45	  111437	  0.59%
 46	  133563	  0.71%
 47	  185334	  0.98%
 48	  137595	  0.73%
 49	  246965	  1.31%
 50	  217315	  1.15%
 51	  114256	  0.61%
 52	   85123	  0.45%
 53	   80471	  0.43%
 54	   90720	  0.48%
 55	   80052	  0.42%
 56	   75930	  0.40%
 57	   61776	  0.33%
 58	   53942	  0.29%
 59	   54738	  0.29%
 60	   59102	  0.31%
 61	   72494	  0.38%
 62	   64999	  0.34%
 63	   65863	  0.35%
 64	   59622	  0.32%
 65	   41940	  0.22%
 66	   47190	  0.25%
 67	   54175	  0.29%
 68	   49354	  0.26%
 69	   41773	  0.22%
 70	   52528	  0.28%
 71	   46053	  0.24%
 72	   49661	  0.26%
 73	   66411	  0.35%
 74	      11	  0.00%
 75	      26	  0.00%
 76	 1012305	  5.36%


criterion=sequence-density
sequence-density=4.61
sequence-density-rank=1
fanout-score=2.73
fanout-score-rank=9
prefix-density=5.09
prefix-fanout=2.5
sequence=TAGCTCAGCTGGGAGAGC


criterion=fanout-score
sequence-density=1.02
sequence-density-rank=11
fanout-score=11.01
fanout-score-rank=1
prefix-density=4.36
prefix-fanout=2.6
sequence=TGTAGCTCAGTTGGT
                                 Started job on |	Dec 28 15:13:07
                             Started mapping on |	Dec 28 15:13:07
                                    Finished on |	Dec 28 15:14:28
       Mapping speed, Million of reads per hour |	863.37

                          Number of input reads |	19425841
                      Average input read length |	34
                                    UNIQUE READS:
                   Uniquely mapped reads number |	3313856
                        Uniquely mapped reads % |	17.06%
                          Average mapped length |	27.15
                       Number of splices: Total |	82362
            Number of splices: Annotated (sjdb) |	7657
                       Number of splices: GT/AG |	77744
                       Number of splices: GC/AG |	1959
                       Number of splices: AT/AC |	82
               Number of splices: Non-canonical |	2577
                      Mismatch rate per base, % |	1.67%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.21
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.02
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	7896588
             % of reads mapped to multiple loci |	40.65%
        Number of reads mapped to too many loci |	2523503
             % of reads mapped to too many loci |	12.99%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	28.89%
                     % of reads unmapped: other |	0.41%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	8215397	8215397	8215397
N_multimapping	7896588	7896588	7896588
N_noFeature	1348818	1551289	3105082
N_ambiguous	22631	16093	247
UnstrandedReadsAssigned:1942407 PositiveStrandReadsAssigned:1746474 NegativeStrandReadsAssigned:208527
Dataset is classified positive stranded
MeadianReadLen=30 20thPercentileLength=22 echo kmer=19
ERR4018153 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 31,715
[index] number of k-mers: 49,362,984
[index] number of equivalence classes: 166,181
[quant] running in single-end mode
[quant] will process file 1: ERR4018153-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 19,425,841 reads, 683,234 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 735 rounds

  31716 ERR4018153.ke.tsv
  32883 ERR4018153.se.tsv
  64599 total
==> ERR4018153.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	856	512	445.442
ENSRNOT00000040993.4	1039	940	866	686.096
ENSRNOT00000050156.3	1545	1446	10599	5458.73
ENSRNOT00000043693.3	684	585	2348	2989.08
ENSRNOT00000046201.3	204	105	29	205.685
ENSRNOT00000046108.3	681	582	1076	1376.84
ENSRNOT00000049683.3	784	685	1030	1119.8
ENSRNOT00000041241.3	348	249	244	729.769
ENSRNOT00000044582.3	297	198	281	1056.9

==> ERR4018153.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	0
ENSRNOG00000058808	0
ENSRNOG00000061316	0
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	0
ERR4018153 completed mapping pipeline successfully
