Starting /dee2/code/volunteer_pipeline.sh ERR4018150
    current disk space = 356860712
    free memory = 198879812 
ERR4018150 SRAfilesize
d73edcf4d41567c445097ca359b021a9  ERR4018150.sra
ERR4018150.sra file validated
ERR4018150 is single end
ERR4018150 is conventional basespace
ERR4018150 read1 length is 76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR4018150_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	76
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.434	32.0	32.0	32.0	32.0	32.0
2	31.27775	32.0	32.0	32.0	32.0	32.0
3	31.5765	32.0	32.0	32.0	32.0	32.0
4	31.632	32.0	32.0	32.0	32.0	32.0
5	31.707	32.0	32.0	32.0	32.0	32.0
6	34.96875	36.0	36.0	36.0	36.0	36.0
7	35.28175	36.0	36.0	36.0	36.0	36.0
8	35.2325	36.0	36.0	36.0	36.0	36.0
9	35.0855	36.0	36.0	36.0	36.0	36.0
10-11	35.332499999999996	36.0	36.0	36.0	36.0	36.0
12-13	35.169624999999996	36.0	36.0	36.0	36.0	36.0
14-15	35.173	36.0	36.0	36.0	36.0	36.0
16-17	35.264	36.0	36.0	36.0	36.0	36.0
18-19	35.2255	36.0	36.0	36.0	36.0	36.0
20-21	35.1975	36.0	36.0	36.0	36.0	36.0
22-23	35.03637500000001	36.0	36.0	36.0	36.0	36.0
24-25	35.048249999999996	36.0	36.0	36.0	36.0	36.0
26-27	35.06275	36.0	36.0	36.0	34.0	36.0
28-29	34.846375	36.0	36.0	36.0	34.0	36.0
30-31	34.94425	36.0	36.0	36.0	34.0	36.0
32-33	34.971999999999994	36.0	36.0	36.0	36.0	36.0
34-35	35.156625	36.0	36.0	36.0	36.0	36.0
36-37	35.019499999999994	36.0	36.0	36.0	36.0	36.0
38-39	34.9465	36.0	36.0	36.0	36.0	36.0
40-41	34.71725	36.0	36.0	36.0	32.0	36.0
42-43	34.901250000000005	36.0	36.0	36.0	36.0	36.0
44-45	35.061375	36.0	36.0	36.0	36.0	36.0
46-47	34.9105	36.0	36.0	36.0	36.0	36.0
48-49	34.682874999999996	36.0	36.0	36.0	32.0	36.0
50-51	34.593625	36.0	36.0	36.0	32.0	36.0
52-53	34.637249999999995	36.0	36.0	36.0	32.0	36.0
54-55	34.210875	36.0	36.0	36.0	32.0	36.0
56-57	33.829499999999996	36.0	36.0	36.0	29.5	36.0
58-59	33.09925	36.0	34.0	36.0	24.0	36.0
60-61	33.416250000000005	36.0	34.0	36.0	27.0	36.0
62-63	32.927875	36.0	32.0	36.0	27.0	36.0
64-65	33.412875	36.0	34.0	36.0	27.0	36.0
66-67	34.148624999999996	36.0	36.0	36.0	32.0	36.0
68-69	34.17425	36.0	36.0	36.0	32.0	36.0
70-71	33.971875	36.0	36.0	36.0	29.5	36.0
72-73	33.81325	36.0	36.0	36.0	29.5	36.0
74-75	34.22875	36.0	36.0	36.0	32.0	36.0
76	33.06875	36.0	32.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	1.0
23	4.0
24	6.0
25	17.0
26	32.0
27	33.0
28	47.0
29	77.0
30	92.0
31	146.0
32	179.0
33	225.0
34	687.0
35	2453.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.300000000000004	19.825	14.6	25.275
2	36.8	35.05	10.475	17.675
3	16.575	25.124999999999996	8.924999999999999	49.375
4	33.1	9.9	39.825	17.175
5	18.025	27.224999999999998	25.900000000000002	28.849999999999998
6	20.538635791593254	11.452303045557514	36.42084067455323	31.588220488296
7	25.900000000000002	16.625	33.35	24.125
8	17.025000000000002	27.474999999999998	47.599999999999994	7.9
9	40.400000000000006	12.55	17.9	29.15
10-11	44.474999999999994	17.962500000000002	18.075	19.4875
12-13	28.050000000000004	23.175	32.5125	16.2625
14-15	42.05	23.8125	16.2125	17.925
16-17	29.2875	30.0875	32.925	7.7
18-19	39.8625	15.137500000000001	26.05	18.95
20-21	34.175	19.5625	35.275	10.9875
22-23	25.624999999999996	27.700000000000003	30.375000000000004	16.3
24-25	31.125000000000004	18.9625	32.800000000000004	17.1125
26-27	18.03975496937117	21.12764095511939	32.00400050006251	28.82860357544693
28-29	33.65023767825869	11.583687765824369	31.123342506880157	23.642732049036777
30-31	29.75743935983996	6.839209802450613	30.857714428607153	32.54563640910227
32-33	43.4625	10.025	23.2625	23.25
34-35	42.1375	21.2625	15.4	21.2
36-37	31.874999999999996	28.549999999999997	22.1	17.474999999999998
38-39	25.6	26.125	16.7375	31.5375
40-41	21.3875	34.637499999999996	14.45	29.525000000000002
42-43	38.3875	24.7375	14.8125	22.0625
44-45	24.4	30.525000000000002	16.7	28.375
46-47	24.1125	20.9125	20.225	34.75
48-49	15.875	28.625	15.037500000000001	40.4625
50-51	30.075000000000003	29.812499999999996	13.225000000000001	26.887499999999996
52-53	25.0625	32.6	21.125	21.212500000000002
54-55	23.45	35.4875	14.45	26.6125
56-57	26.375	27.375	23.0	23.25
58-59	23.575	30.7375	21.25	24.4375
60-61	26.674999999999997	24.6	26.724999999999998	22.0
62-63	21.912499999999998	23.3875	30.2125	24.4875
64-65	19.2125	17.175	33.625	29.9875
66-67	28.1125	17.0375	29.225	25.624999999999996
68-69	28.849999999999998	21.875	26.9625	22.3125
70-71	26.437500000000004	17.2375	31.112499999999997	25.2125
72-73	18.875	8.8125	37.35	34.9625
74-75	16.7875	7.1625	40.8375	35.212500000000006
76	27.400000000000002	7.575	39.225	25.8
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.5
25	1.0
26	0.5
27	0.0
28	0.0
29	0.0
30	0.0
31	0.5
32	1.0
33	1.0
34	1.0
35	2.5
36	3.0
37	2.0
38	3.0
39	3.5
40	3.5
41	5.5
42	7.0
43	10.0
44	16.5
45	28.0
46	36.0
47	81.5
48	216.5
49	387.5
50	469.0
51	474.5
52	730.0
53	761.5
54	543.0
55	443.0
56	306.5
57	193.0
58	116.0
59	163.0
60	127.5
61	27.0
62	9.0
63	5.5
64	1.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.5
71	1.0
72	0.5
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.675
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0125
28-29	0.075
30-31	0.025
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.66349670811998	27.224999999999998
2	8.119970738844184	5.55
3	2.852962692026335	2.9250000000000003
4	1.4630577907827358	2.0
5	1.3899049012435991	2.375
6	0.8046817849305048	1.6500000000000001
7	0.36576444769568395	0.8750000000000001
8	0.5120702267739575	1.4000000000000001
9	0.2926115581565472	0.8999999999999999
>10	3.8771031455742504	24.525
>50	0.2926115581565472	7.3
>100	0.36576444769568395	23.275000000000002
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GACTCTTAGCGGTGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	392	9.8	RNA PCR Primer, Index 1 (100% over 33bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	153	3.8249999999999997	No Hit
ACAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG	150	3.75	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA	128	3.2	No Hit
CGCGACCTCAGATCAGACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	108	2.7	RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTCAGATCAGACGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	83	2.075	RNA PCR Primer, Index 1 (100% over 30bp)
GACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	82	2.0500000000000003	RNA PCR Primer, Index 1 (100% over 32bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA	74	1.8499999999999999	RNA PCR Primer, Index 1 (100% over 28bp)
GACTCTTAGCGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTA	53	1.325	RNA PCR Primer, Index 3 (97% over 36bp)
AGCTACATTGTCTGCTGGGTTTCTGGAATTCTCGGGTGCCAAGGAACTCC	47	1.175	RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	46	1.15	RNA PCR Primer, Index 1 (100% over 32bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCTGGAATTCTCGGGTGC	45	1.125	No Hit
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 29bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGTGGAATTCTCGGGTGCCAAG	32	0.8	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	31	0.775	No Hit
TGGCTCAGTTCAGCAGGAACAGTGGAATTCTCGGGTGCCAAGGAACTCCA	30	0.75	RNA PCR Primer, Index 1 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA	29	0.7250000000000001	No Hit
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	27	0.675	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG	25	0.625	No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCTGGAATTCTCGGGTGCCAA	24	0.6	No Hit
CAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	23	0.575	RNA PCR Primer, Index 1 (100% over 30bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCTGGAATTCTCGGGTG	21	0.525	No Hit
GACTCTTAGCGGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT	21	0.525	RNA PCR Primer, Index 1 (97% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA	20	0.5	RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG	20	0.5	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC	20	0.5	RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA	20	0.5	No Hit
GACTCTTAGCGGTGGATCACTTGGAATTCTCGGGTGCCAAGGAACTCCAG	19	0.475	RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAG	19	0.475	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC	19	0.475	No Hit
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCT	19	0.475	RNA PCR Primer, Index 17 (100% over 43bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGTGGAATTCTCGGGTGCCA	18	0.44999999999999996	No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 27bp)
GGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCAAGG	17	0.42500000000000004	Illumina Small RNA Adapter 2 (100% over 21bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT	17	0.42500000000000004	No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	16	0.4	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	16	0.4	RNA PCR Primer, Index 1 (100% over 33bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT	15	0.375	RNA PCR Primer, Index 1 (97% over 35bp)
GTTTCCGTAGTGTAGTGGTTATCTGGAATTCTCGGGTGCCAAGGAACTCC	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTA	13	0.325	RNA PCR Primer, Index 3 (97% over 36bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCTGGAATTCTCGGGTGCCAAGGAACTCC	13	0.325	RNA PCR Primer, Index 1 (100% over 27bp)
GACTCTTAGCGGTGGATCACTCTGGAATTCTCGGGTGCCAAGGAACTCCA	13	0.325	RNA PCR Primer, Index 1 (100% over 28bp)
GGCCGCCGGTGAAATACCACTACTCTGGAATTCTCGGGTGCCAAGGAACT	12	0.3	RNA PCR Primer, Index 1 (100% over 25bp)
ATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT	12	0.3	RNA PCR Primer, Index 1 (97% over 35bp)
TGGAAGACTAGTGATTTTGTTGTTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC	12	0.3	RNA PCR Primer, Index 1 (100% over 27bp)
GACTCTTAGCGGTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	11	0.27499999999999997	RNA PCR Primer, Index 6 (100% over 34bp)
ACAGTAGTCTGCACATTGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGTGGAATTCTCGGGTGCCAA	11	0.27499999999999997	No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAA	11	0.27499999999999997	No Hit
ACAGTAGTCTGCACATTGGTTATGGAATTCTCGGGTGCCAAGGAACTCCA	10	0.25	RNA PCR Primer, Index 1 (100% over 28bp)
GGCCGCCGGTGAAATACCACTACTCTCATGGAATTCTCGGGTGCCAAGGA	10	0.25	RNA PCR Primer, Index 1 (100% over 22bp)
ACTCTTAGCGGTGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	10	0.25	RNA PCR Primer, Index 6 (100% over 34bp)
AACATTCATTGCTGTCGGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTC	10	0.25	RNA PCR Primer, Index 1 (100% over 26bp)
TGGATTTTTGGAACTAGGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	10	0.25	RNA PCR Primer, Index 1 (100% over 29bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTA	10	0.25	RNA PCR Primer, Index 3 (97% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC	10	0.25	No Hit
CGACTCTTAGCGGTGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	10	0.25	RNA PCR Primer, Index 1 (100% over 32bp)
CGACTCTTAGCGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (97% over 35bp)
GGCCGCCGGTGAAATACCACTACTTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
AAATGGATTTTTGGAACTAGGAGTTGGAATTCTCGGGTGCCAAGGAACTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 26bp)
CGACTCTTAGCGGTGGATCACTTGGAATTCTCGGGTGCCAAGGAACTCCA	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC	8	0.2	No Hit
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA	8	0.2	RNA PCR Primer, Index 1 (100% over 23bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTTGGAATTCTCGGGTGCCAAGG	8	0.2	Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
GTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCA	8	0.2	RNA PCR Primer, Index 1 (100% over 28bp)
AATGGATTTTTGGAACTAGGAGTTGGAATTCTCGGGTGCCAAGGAACTCC	8	0.2	RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCTGGAATTCTCGGGTGCCA	7	0.17500000000000002	No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA	7	0.17500000000000002	No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCATGG	7	0.17500000000000002	No Hit
ATCCCGGGGCCGAGGGAGCCGGATTGGAATTCTCGGGTGCCAAGGAACTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 26bp)
ATGTAGGTAAGGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	6	0.15	RNA PCR Primer, Index 6 (100% over 34bp)
AACATTCAACGCTGTCGGTGAGTTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
GGCCGCCGGTGAAATACCACTACTCTCTGGAATTCTCGGGTGCCAAGGAA	6	0.15	RNA PCR Primer, Index 1 (100% over 23bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	6	0.15	RNA PCR Primer, Index 1 (100% over 28bp)
AGGCAGTGTAGTTAGCTGATTGCTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGAT	6	0.15	RNA PCR Primer, Index 17 (100% over 41bp)
ACAGTAGTCTGCACATTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
ATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGG	6	0.15	Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGA	6	0.15	RNA PCR Primer, Index 17 (100% over 38bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTTGGAATTCTCGGGTGCCAAG	6	0.15	No Hit
TGGTGCGGACCAGGGGAATCCGACTGTTTGGAATTCTCGGGTGCCAAGGA	6	0.15	RNA PCR Primer, Index 1 (100% over 22bp)
GACCCGCGGGCGCTCTCTGGCCCTTTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
GAGGTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
AAATGGATTTTTGGAACTAGGAGTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGGAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GACTCTTAGCGGTGGATCACTCGGCTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
CGACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
ATGTAGGTAAGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAG	5	0.125	RNA PCR Primer, Index 3 (97% over 37bp)
TGGATTTTTGGAACTAGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
GGTGCGGACCAGGGGAATCCGACTGTTTGGAATTCTCGGGTGCCAAGGAA	5	0.125	RNA PCR Primer, Index 1 (100% over 23bp)
TGAGGTAGTAGGTTGTGTGGTTTTGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ACTAACCCTAGCCCTACAACCAACCTGGAATTCTCGGGTGCCAAGGAACT	5	0.125	RNA PCR Primer, Index 1 (100% over 25bp)
ATCCCGGGGCCGAGGGAGCCGGATGGAATTCTCGGGTGCCAAGGAACTCC	5	0.125	RNA PCR Primer, Index 1 (100% over 27bp)
ATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGG	5	0.125	Illumina Small RNA Adapter 2 (100% over 21bp)
AGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
ATCCCGGGGCCGAGGGAGCCGGATACTGGAATTCTCGGGTGCCAAGGAAC	5	0.125	RNA PCR Primer, Index 1 (100% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.025	0.0	0.0	0.0
4	0.0	0.025	0.0	0.0	0.0
5	0.0	0.075	0.0	0.0	0.0
6	0.0	0.125	0.0	0.0	0.0
7	0.0	0.4	0.0	0.0	0.0
8	0.0	1.325	0.0	0.0	0.0
9	0.0	1.65	0.0	0.0	0.0
10	0.0	2.35	0.0	0.0	0.0
11	0.0	2.95	0.0	0.0	0.0
12	0.0	3.4	0.0	0.0	0.0
13	0.0	4.1	0.0	0.0	0.0
14	0.0	4.8	0.0	0.0	0.0
15	0.0	7.775	0.0	0.0	0.0
16	0.0	9.9	0.0	0.0	0.0
17	0.0	11.7	0.0	0.0	0.0
18	0.0	23.525	0.0	0.0	0.0
19	0.0	28.275	0.0	0.0	0.0
20	0.0	33.1	0.0	0.0	0.0
21	0.0	37.8	0.0	0.0	0.0
22	0.0	46.325	0.0	0.0	0.0
23	0.0	55.85	0.0	0.0	0.0
24	0.0	62.95	0.0	0.0	0.0
25	0.0	65.7	0.0	0.0	0.0
26	0.0	67.35	0.0	0.0	0.0
27	0.0	68.525	0.0	0.0	0.0
28	0.0	69.775	0.0	0.0	0.0
29	0.0	71.45	0.0	0.0	0.0
30	0.0	72.975	0.0	0.0	0.0
31	0.0	76.275	0.0	0.0	0.0
32	0.0	86.075	0.0	0.0	0.0
33	0.0	88.775	0.0	0.0	0.0
34	0.0	90.325	0.0	0.0	0.0
35	0.0	92.1	0.0	0.0	0.0
36	0.0	92.975	0.0	0.0	0.0
37	0.0	93.375	0.0	0.0	0.0
38	0.0	93.625	0.0	0.0	0.0
39	0.0	93.85	0.0	0.0	0.0
40	0.0	94.025	0.0	0.0	0.0
41	0.0	94.225	0.0	0.0	0.0
42	0.0	94.425	0.0	0.0	0.0
43	0.0	94.55	0.0	0.0	0.0
44	0.0	94.625	0.0	0.0	0.0
45	0.0	94.85	0.0	0.0	0.0
46	0.0	94.9	0.0	0.0	0.0
47	0.0	95.025	0.0	0.0	0.0
48	0.0	95.175	0.0	0.0	0.0
49	0.0	95.225	0.0	0.0	0.0
50	0.0	95.575	0.0	0.0	0.0
51	0.0	95.975	0.0	0.0	0.0
52	0.0	96.025	0.0	0.0	0.0
53	0.0	96.05	0.0	0.0	0.0
54	0.0	96.075	0.0	0.0	0.0
55	0.0	96.125	0.0	0.0	0.0
56	0.0	96.125	0.0	0.0	0.0
57	0.0	96.15	0.0	0.0	0.0
58	0.0	96.15	0.0	0.0	0.0
59	0.0	96.175	0.0	0.0	0.0
60	0.0	96.25	0.0	0.0	0.0
61	0.0	96.275	0.0	0.0	0.0
62	0.0	96.3	0.0	0.0	0.0
63	0.0	96.325	0.0	0.0	0.0
64	0.0	96.375	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGGTAG	15	0.0020658788	70.00001	2
TGGTTCA	45	3.6379788E-12	70.00001	8
GGGTTTC	15	0.0020658788	70.00001	17
TGGTAGA	45	3.6379788E-12	70.00001	16
GCGACCC	30	7.878043E-8	70.00001	22
TTTCTGG	15	0.0020658788	70.00001	20
CCGCTTG	15	0.0020658788	70.00001	27
CCGCCGG	15	0.0020658788	70.00001	3
GGCCGCC	15	0.0020658788	70.00001	1
AGTGGTA	45	3.6379788E-12	70.00001	14
CGCTTGG	15	0.0020658788	70.00001	28
TTCTGGA	15	0.0020658788	70.00001	21
GTTTCTG	15	0.0020658788	70.00001	19
GGCGACC	30	7.878043E-8	70.00001	21
GGTTTCT	15	0.0020658788	70.00001	18
GGTTCAG	45	3.6379788E-12	70.00001	9
GTTCAGT	45	3.6379788E-12	70.00001	10
AGAATTC	45	3.6379788E-12	70.00001	20
CCCGCTT	15	0.0020658788	70.00001	26
CGTGGCG	30	7.878043E-8	70.00001	18
>>END_MODULE
Rejected 2875641 READS because READLEN < 1
Read 2875641 spots for ERR4018150.sra
Written 2875641 spots for ERR4018150.sra
Rejected 2875641 READS because READLEN < 1
Read 2875641 spots for ERR4018150.sra
Written 2875641 spots for ERR4018150.sra
Rejected 2875641 READS because READLEN < 1
Read 2875641 spots for ERR4018150.sra
Written 2875641 spots for ERR4018150.sra
Rejected 2875641 READS because READLEN < 1
Read 2875641 spots for ERR4018150.sra
Written 2875641 spots for ERR4018150.sra
Rejected 2875641 READS because READLEN < 1
Read 2875641 spots for ERR4018150.sra
Written 2875641 spots for ERR4018150.sra
Rejected 2875641 READS because READLEN < 1
Read 2875641 spots for ERR4018150.sra
Written 2875641 spots for ERR4018150.sra
Rejected 2875641 READS because READLEN < 1
Read 2875641 spots for ERR4018150.sra
Written 2875641 spots for ERR4018150.sra
Rejected 2875642 READS because READLEN < 1
Read 2875642 spots for ERR4018150.sra
Written 2875642 spots for ERR4018150.sra
SRR ids: ['ERR4018150.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_792y0064
ERR4018150.sra spots: 23005129
blocks: [[1, 2875641], [2875642, 5751282], [5751283, 8626923], [8626924, 11502564], [11502565, 14378205], [14378206, 17253846], [17253847, 20129487], [20129488, 23005129]]
ERR4018150 file size 4381632
ERR4018150 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o ERR4018150 ERR4018150_1.fastq
Input file:	ERR4018150_1.fastq
trimmed:	ERR4018150-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Sun Dec 28 15:07:40 2025 >> started

Sun Dec 28 15:07:53 2025 >> done (12.433s)
23005129 reads processed; of these:
       0 ( 0.00%) short reads filtered out after trimming by size control
       0 ( 0.00%) empty reads filtered out after trimming by size control
23005129 (100.00%) reads available; of these:
    4265 ( 0.02%) trimmed reads available after processing
23000864 (99.98%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 42	      76	  0.00%
 43	     110	  0.00%
 44	      91	  0.00%
 45	     474	  0.00%
 46	     550	  0.00%
 47	     595	  0.00%
 48	     489	  0.00%
 49	     579	  0.00%
 50	     158	  0.00%
 51	     264	  0.00%
 52	     400	  0.00%
 53	      20	  0.00%
 54	     162	  0.00%
 55	      13	  0.00%
 56	       5	  0.00%
 57	      18	  0.00%
 58	       1	  0.00%
 59	       0	  0.00%
 60	       0	  0.00%
 61	       5	  0.00%
 62	       0	  0.00%
 63	       3	  0.00%
 64	       0	  0.00%
 65	       0	  0.00%
 66	       0	  0.00%
 67	      34	  0.00%
 68	      14	  0.00%
 69	      68	  0.00%
 70	       7	  0.00%
 71	       2	  0.00%
 72	       3	  0.00%
 73	      47	  0.00%
 74	       7	  0.00%
 75	      70	  0.00%
 76	23000864	 99.98%
23005129 reads passed initial QC


criterion=sequence-density
sequence-density=96.81
sequence-density-rank=1
fanout-score=24.67
fanout-score-rank=1
prefix-density=97.49
prefix-fanout=24.5
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAA


criterion=fanout-score
sequence-density=96.81
sequence-density-rank=1
fanout-score=24.67
fanout-score-rank=1
prefix-density=97.49
prefix-fanout=24.5
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAA
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAA -o ERR4018150 -
Input file:	STDIN
trimmed:	ERR4018150-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGCCGTCTTCTGCTTGA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Sun Dec 28 15:08:14 2025 >> started

Sun Dec 28 15:08:35 2025 >> done (21.388s)
22530797 reads processed; of these:
 5523344 (24.51%) short reads filtered out after trimming by size control
     437 ( 0.00%) empty reads filtered out after trimming by size control
17007016 (75.48%) reads available; of these:
16769976 (98.61%) trimmed reads available after processing
  237040 ( 1.39%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	 1046007	  6.15%
 19	 1193969	  7.02%
 20	 1043960	  6.14%
 21	 1762777	 10.36%
 22	 2275075	 13.38%
 23	 1470245	  8.64%
 24	  787271	  4.63%
 25	  554368	  3.26%
 26	  300525	  1.77%
 27	  298705	  1.76%
 28	  433498	  2.55%
 29	  367493	  2.16%
 30	  835313	  4.91%
 31	 2132016	 12.54%
 32	  570201	  3.35%
 33	  325671	  1.91%
 34	  357391	  2.10%
 35	  231457	  1.36%
 36	   79186	  0.47%
 37	   54008	  0.32%
 38	   47457	  0.28%
 39	   42811	  0.25%
 40	   30186	  0.18%
 41	   31304	  0.18%
 42	   37665	  0.22%
 43	   28137	  0.17%
 44	   35073	  0.21%
 45	   22154	  0.13%
 46	   21789	  0.13%
 47	   32118	  0.19%
 48	   23641	  0.14%
 49	   81860	  0.48%
 50	   61808	  0.36%
 51	   12836	  0.08%
 52	    6565	  0.04%
 53	    6139	  0.04%
 54	    6355	  0.04%
 55	    5629	  0.03%
 56	    6163	  0.04%
 57	   13831	  0.08%
 58	   10703	  0.06%
 59	    8220	  0.05%
 60	    6758	  0.04%
 61	    8987	  0.05%
 62	    5646	  0.03%
 63	    4932	  0.03%
 64	    5618	  0.03%
 65	    3753	  0.02%
 66	    7487	  0.04%
 67	    5273	  0.03%
 68	    6125	  0.04%
 69	    3172	  0.02%
 70	    3586	  0.02%
 71	    7933	  0.05%
 72	    4155	  0.02%
 73	    5106	  0.03%
 74	       2	  0.00%
 75	      37	  0.00%
 76	  236866	  1.39%


criterion=sequence-density
sequence-density=6.20
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=7
prefix-density=0.00
prefix-fanout=1.0
sequence=GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCA


criterion=fanout-score
sequence-density=0.34
sequence-density-rank=20
fanout-score=3.68
fanout-score-rank=1
prefix-density=1.27
prefix-fanout=1.0
sequence=TAGTAGGTTGTGTGGTTT
                                 Started job on |	Dec 28 15:08:45
                             Started mapping on |	Dec 28 15:08:45
                                    Finished on |	Dec 28 15:09:44
       Mapping speed, Million of reads per hour |	1066.66

                          Number of input reads |	17481348
                      Average input read length |	27
                                    UNIQUE READS:
                   Uniquely mapped reads number |	3197626
                        Uniquely mapped reads % |	18.29%
                          Average mapped length |	24.71
                       Number of splices: Total |	12237
            Number of splices: Annotated (sjdb) |	4969
                       Number of splices: GT/AG |	10496
                       Number of splices: GC/AG |	152
                       Number of splices: AT/AC |	26
               Number of splices: Non-canonical |	1563
                      Mismatch rate per base, % |	0.24%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.06
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.49
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	7375464
             % of reads mapped to multiple loci |	42.19%
        Number of reads mapped to too many loci |	6155336
             % of reads mapped to too many loci |	35.21%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	3.97%
                     % of reads unmapped: other |	0.34%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	6908258	6908258	6908258
N_multimapping	7375464	7375464	7375464
N_noFeature	174352	285173	3082715
N_ambiguous	83978	79890	20
UnstrandedReadsAssigned:2939296 PositiveStrandReadsAssigned:2832563 NegativeStrandReadsAssigned:114891
Dataset is classified positive stranded
MeadianReadLen=23 20thPercentileLength=21 echo kmer=19
ERR4018150 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 31,715
[index] number of k-mers: 49,362,984
[index] number of equivalence classes: 166,181
[quant] running in single-end mode
[quant] will process file 1: ERR4018150-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 17,481,348 reads, 1,873,269 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 682 rounds

  31716 ERR4018150.ke.tsv
  32883 ERR4018150.se.tsv
  64599 total
==> ERR4018150.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	856	125	35.5067
ENSRNOT00000040993.4	1039	940	192	49.6647
ENSRNOT00000050156.3	1545	1446	1924	323.528
ENSRNOT00000043693.3	684	585	623	258.945
ENSRNOT00000046201.3	204	105	83	192.204
ENSRNOT00000046108.3	681	582	196	81.8856
ENSRNOT00000049683.3	784	685	251	89.0959
ENSRNOT00000041241.3	348	249	51	49.8018
ENSRNOT00000044582.3	297	198	93	114.207

==> ERR4018150.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	0
ENSRNOG00000058808	0
ENSRNOG00000061316	0
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	0
ERR4018150 completed mapping pipeline successfully
