Starting /dee2/code/volunteer_pipeline.sh ERR6357085
    current disk space = 186836744
    free memory = 191883928 
ERR6357085 SRAfilesize
7b11f3b0f09e993697ed944e5278b6aa  ERR6357085.sra
ERR6357085.sra file validated
ERR6357085 is paired end
ERR6357085 is conventional basespace
ERR6357085 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR6357085_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58625	32.0	32.0	32.0	32.0	32.0
2	31.4775	32.0	32.0	32.0	32.0	32.0
3	35.395	37.0	37.0	37.0	32.0	37.0
4	36.0075	37.0	37.0	37.0	32.0	37.0
5	36.465	37.0	37.0	37.0	37.0	37.0
6	39.93475	41.0	41.0	41.0	37.0	41.0
7	40.0265	41.0	41.0	41.0	37.0	41.0
8	40.214	41.0	41.0	41.0	37.0	41.0
9	40.187	41.0	41.0	41.0	37.0	41.0
10-14	40.07	41.0	41.0	41.0	37.0	41.0
15-19	39.949200000000005	41.0	41.0	41.0	37.0	41.0
20-24	39.7805	41.0	41.0	41.0	37.0	41.0
25-29	39.43095	41.0	41.0	41.0	37.0	41.0
30-34	39.2147	41.0	41.0	41.0	37.0	41.0
35-39	38.910349999999994	41.0	41.0	41.0	37.0	41.0
40-44	38.88520000000001	41.0	41.0	41.0	36.0	41.0
45-49	38.5726	41.0	41.0	41.0	32.0	41.0
50-54	38.31855	41.0	38.6	41.0	32.0	41.0
55-59	38.04965	41.0	37.0	41.0	30.0	41.0
60-64	37.7859	41.0	37.0	41.0	27.0	41.0
65-69	37.53150000000001	41.0	37.0	41.0	27.0	41.0
70-74	37.0632	41.0	37.0	41.0	27.0	41.0
75-79	37.007349999999995	41.0	37.0	41.0	27.0	41.0
80-84	37.2955	41.0	37.0	41.0	27.0	41.0
85-89	37.19	41.0	37.0	41.0	27.0	41.0
90-94	36.800850000000004	41.0	37.0	41.0	23.0	41.0
95-99	36.337450000000004	41.0	37.0	41.0	22.0	41.0
100-104	35.986	41.0	35.0	41.0	22.0	41.0
105-109	35.591699999999996	41.0	32.0	41.0	22.0	41.0
110-114	35.343849999999996	41.0	32.0	41.0	22.0	41.0
115-119	34.92065	41.0	32.0	41.0	20.0	41.0
120-124	34.50335	41.0	32.0	41.0	14.0	41.0
125-129	34.08985	40.2	31.0	41.0	12.0	41.0
130-134	33.546350000000004	37.0	28.0	41.0	12.0	41.0
135-139	32.927800000000005	37.0	27.0	41.0	12.0	41.0
140-144	32.4373	37.0	25.0	41.0	12.0	41.0
145-149	31.9746	37.0	22.0	41.0	12.0	41.0
150	31.48275	37.0	22.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	1.0
20	4.0
21	14.0
22	18.0
23	21.0
24	31.0
25	49.0
26	44.0
27	47.0
28	41.0
29	74.0
30	72.0
31	96.0
32	114.0
33	142.0
34	178.0
35	228.0
36	343.0
37	463.0
38	561.0
39	617.0
40	839.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.66041510377595	10.802700675168792	7.851962990747687	39.68492123030758
2	32.4	16.175	32.324999999999996	19.1
3	32.574999999999996	21.25	20.525	25.650000000000002
4	31.924999999999997	31.7	14.625	21.75
5	32.1	33.525	16.475	17.9
6	26.6	32.775	15.675	24.95
7	26.775	16.025	31.924999999999997	25.275
8	23.525	18.6	27.224999999999998	30.65
9	26.6	19.45	26.125	27.825
10-14	28.825	23.73	21.9	25.545
15-19	30.36	22.27	21.935	25.435000000000002
20-24	28.715000000000003	22.705000000000002	22.415	26.165
25-29	28.88	24.575	20.145	26.400000000000002
30-34	27.634999999999998	24.325	20.335	27.705000000000002
35-39	28.799999999999997	24.355	20.22	26.625
40-44	27.83	22.295	22.314999999999998	27.560000000000002
45-49	27.544999999999998	22.535	22.715	27.205000000000002
50-54	27.445000000000004	21.67	23.235	27.650000000000002
55-59	28.1	22.745	22.105	27.05
60-64	28.749999999999996	22.62	22.945	25.685000000000002
65-69	26.695	21.875	22.475	28.955
70-74	27.74	22.025	21.825	28.410000000000004
75-79	28.395	22.53	22.255	26.82
80-84	27.46	23.165	22.040000000000003	27.334999999999997
85-89	28.165000000000003	22.1	22.58	27.155
90-94	27.315	22.7	22.07	27.915
95-99	28.325	22.8	21.465	27.41
100-104	27.35	23.455000000000002	21.925	27.27
105-109	28.285	21.555	22.015	28.144999999999996
110-114	27.615000000000002	22.375	21.725	28.285
115-119	27.46	23.025000000000002	21.78	27.735
120-124	27.77	23.330000000000002	22.015	26.884999999999998
125-129	27.21	23.91	21.8	27.08
130-134	28.125	23.235	22.98	25.66
135-139	26.245	24.36	21.23	28.165000000000003
140-144	26.995	23.27	23.35	26.384999999999998
145-149	26.43	24.834999999999997	20.560000000000002	28.175
150	23.599999999999998	25.85	20.724999999999998	29.825000000000003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	1.0
23	0.5
24	0.0
25	0.0
26	0.0
27	0.0
28	0.5
29	1.5
30	1.0
31	1.0
32	1.0
33	2.0
34	3.5
35	3.5
36	6.0
37	11.0
38	11.0
39	11.0
40	10.0
41	5.0
42	7.5
43	13.5
44	18.5
45	21.5
46	22.0
47	25.0
48	43.0
49	94.0
50	203.0
51	285.5
52	278.5
53	238.0
54	207.5
55	189.5
56	240.5
57	378.5
58	444.5
59	419.0
60	294.0
61	165.5
62	114.5
63	76.0
64	47.0
65	19.5
66	11.0
67	6.5
68	3.5
69	3.5
70	5.0
71	5.5
72	4.5
73	3.0
74	2.5
75	3.0
76	3.0
77	2.5
78	4.5
79	6.0
80	5.5
81	4.0
82	2.5
83	1.5
84	2.5
85	2.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.00907029478458	41.9
2	9.61451247165533	10.6
3	5.170068027210884	8.55
4	2.811791383219955	6.2
5	1.3151927437641724	3.6249999999999996
6	1.0430839002267573	3.45
7	0.8616780045351473	3.325
8	0.6349206349206349	2.8000000000000003
9	0.27210884353741494	1.35
>10	2.2675736961451247	18.2
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCC	38	0.95	No Hit
GGAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGG	32	0.8	No Hit
GTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACC	25	0.625	No Hit
AGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATT	19	0.475	No Hit
CCTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATG	18	0.44999999999999996	No Hit
TGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGAC	18	0.44999999999999996	No Hit
GACAAAACCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATGGTGG	18	0.44999999999999996	No Hit
CTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTT	18	0.44999999999999996	No Hit
GGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCAC	17	0.42500000000000004	No Hit
GGAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCT	17	0.42500000000000004	No Hit
GTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCT	16	0.4	No Hit
GGGGAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGA	16	0.4	No Hit
GCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTC	16	0.4	No Hit
GGGACCTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCC	16	0.4	No Hit
CCCGGCTCTGCCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTT	16	0.4	No Hit
GGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACGG	15	0.375	No Hit
CTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTGACAAGCTGCA	15	0.375	No Hit
GTTGTGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTG	15	0.375	No Hit
GGAAAAGAGGTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCAGC	15	0.375	No Hit
GGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGG	15	0.375	No Hit
CTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTG	14	0.35000000000000003	No Hit
GTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCAT	14	0.35000000000000003	No Hit
GCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCC	14	0.35000000000000003	No Hit
GGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACA	14	0.35000000000000003	No Hit
GTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTAT	14	0.35000000000000003	No Hit
GTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCA	13	0.325	No Hit
CTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGT	13	0.325	No Hit
CAGGAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAA	13	0.325	No Hit
CTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCA	13	0.325	No Hit
GGCGGCTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGA	12	0.3	No Hit
CCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTGCCCAGGTC	12	0.3	No Hit
CGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCTGCAGACCACGTCGAAG	12	0.3	No Hit
AAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACTGC	12	0.3	No Hit
GGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTT	12	0.3	No Hit
CTCGGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCAT	12	0.3	No Hit
GGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACT	12	0.3	No Hit
GCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACAGGATC	11	0.27499999999999997	No Hit
CTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATGG	11	0.27499999999999997	No Hit
CCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATGGTGGTGAATAT	11	0.27499999999999997	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC	11	0.27499999999999997	TruSeq Adapter, Index 9 (100% over 50bp)
CTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCTGCTATCATGGGTAACC	11	0.27499999999999997	No Hit
GTCAACTTCAAGTTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTTGCCA	11	0.27499999999999997	No Hit
TGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGC	11	0.27499999999999997	No Hit
GTGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATG	10	0.25	No Hit
CTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCT	10	0.25	No Hit
CCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTGTCTACC	10	0.25	No Hit
AGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGTC	10	0.25	No Hit
CCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACC	10	0.25	No Hit
CTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAA	10	0.25	No Hit
GTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTGT	10	0.25	No Hit
GTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGCT	9	0.22499999999999998	No Hit
CTGGTTGTCTACCCTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCT	9	0.22499999999999998	No Hit
CGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCT	9	0.22499999999999998	No Hit
CCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCTGCTATC	9	0.22499999999999998	No Hit
GTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCAGCCACCACCTT	9	0.22499999999999998	No Hit
CCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGC	9	0.22499999999999998	No Hit
CGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGAGGTACT	8	0.2	No Hit
GGAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACT	8	0.2	No Hit
GGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTG	8	0.2	No Hit
GAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATTG	8	0.2	No Hit
CTTCTCTCACATTGATGTAAGCCCCGGCTCTGCCCAGGTCAAGGCTCACG	8	0.2	No Hit
GTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTC	8	0.2	No Hit
CAGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGT	8	0.2	No Hit
CCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCT	8	0.2	No Hit
GGAGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATGTTGC	8	0.2	No Hit
GAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCC	8	0.2	No Hit
CTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAA	8	0.2	No Hit
GCCCTGTCCACTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCC	8	0.2	No Hit
GTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCC	8	0.2	No Hit
GTGCTCTCTGCAGATGACAAAACCAACATCAAGAACTGCTGGGGGAAGAT	8	0.2	No Hit
CGCCTTCAATGATGGCCTGAAACACTTGGACAACCTCAAGGGCACCTTTG	7	0.17500000000000002	No Hit
CTTGGGAACAATTGACCAATGCACAAAGACAAGAGCAGGAAAAGAGGTTT	7	0.17500000000000002	No Hit
GGTAACCCTAAGGTGAAGGCCCATGGCAAGAAGGTGATAAACGCCTTCAA	7	0.17500000000000002	No Hit
GTCGAAGACCTGCCTGGTGCCCTGTCCACTCTGAGCGACCTGCATGCCCA	7	0.17500000000000002	No Hit
CACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGCTGAGAAGG	7	0.17500000000000002	No Hit
GGCTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAATT	7	0.17500000000000002	No Hit
CTAAGGTGAAGGCCCATGGCAAGAAGGTGATAAACGCCTTCAATGATGGC	7	0.17500000000000002	No Hit
GAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTG	7	0.17500000000000002	No Hit
CACAAACTGCGTGTGGATCCTGTCAACTTCAAGTTCCTGAGCCACTGCCT	7	0.17500000000000002	No Hit
CAAGAAGGTGATAAACGCCTTCAATGATGGCCTGAAACACTTGGACAACC	7	0.17500000000000002	No Hit
GAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCA	7	0.17500000000000002	No Hit
CTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGGGGAAGGCAGCGAA	7	0.17500000000000002	No Hit
GGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGAC	7	0.17500000000000002	No Hit
GCGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGAGGTAC	7	0.17500000000000002	No Hit
CTGATGCCTTGGCCAAAGCTGCAGACCACGTCGAAGACCTGCCTGGTGCC	7	0.17500000000000002	No Hit
GTCAGCACAGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGGCGTGCAT	7	0.17500000000000002	No Hit
CATAGTTGTGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTG	7	0.17500000000000002	No Hit
ATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAG	7	0.17500000000000002	No Hit
CTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCT	7	0.17500000000000002	No Hit
CTGCAGATGACAAAACCAACATCAAGAACTGCTGGGGGAAGATTGGTGGC	6	0.15	No Hit
CGAAGACCTGCCTGGTGCCCTGTCCACTCTGAGCGACCTGCATGCCCACA	6	0.15	No Hit
GGGGAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTG	6	0.15	No Hit
CTGCTGGTTGTCTACCCTTGGACCCAGAGGTACTTTGATAGCTTTGGGGA	6	0.15	No Hit
GGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACG	6	0.15	No Hit
GTGCACTCGGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACA	6	0.15	No Hit
GAACAATTGACCATTGCACAAAGACAAGAGCAGGAAAAGAGGTTTAGTGG	6	0.15	No Hit
CCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTC	6	0.15	No Hit
CTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTTCTTGCCG	6	0.15	No Hit
CTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAATTTG	6	0.15	No Hit
CCGGGGCTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGGGGAAGGC	6	0.15	No Hit
GGGAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGC	6	0.15	No Hit
AGGATCCACACGCAGTTTGTGGGCATGCAGGTCGCTCAGAGTGGACAGGG	6	0.15	No Hit
GTGAGCCTTGACCTGGGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGT	6	0.15	No Hit
TGTGGATCCTGTCAACTTCAAGTTCCTGAGCCACTGCCTGCTGGTGACCT	6	0.15	No Hit
GCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTG	6	0.15	No Hit
GCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACAG	6	0.15	No Hit
GTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGT	6	0.15	No Hit
GCCTGAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAA	6	0.15	No Hit
GGGTAACCCTAAGGTGAAGGCCCATGGCAAGAAGGTGATAAACGCCTTCA	6	0.15	No Hit
GTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCT	6	0.15	No Hit
GGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGC	6	0.15	No Hit
CCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGG	6	0.15	No Hit
GCTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGGGGAAGGCAGCGA	5	0.125	No Hit
CAAAGACAAGAGCAGGAAAAGAGGTTTAGTGGTACTTGTGAGCCAGGGCA	5	0.125	No Hit
GCTGATGCCTTGGCCAAAGCTGCAGACCACGTCGAAGACCTGCCTGGTGC	5	0.125	No Hit
CTGCCTTCCAGAAGGTGGTGGCTGGAGTGGCCAGTGCCCTGGCTCACAAG	5	0.125	No Hit
AGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACGGTAC	5	0.125	No Hit
GGCGTGCATGGCGGGTGTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCA	5	0.125	No Hit
TGGTTGTCTACCCTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCTG	5	0.125	No Hit
GTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGG	5	0.125	No Hit
CTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATC	5	0.125	No Hit
CTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGG	5	0.125	No Hit
ATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGT	5	0.125	No Hit
GGAATTTGTCCAGAGAGGCGTGCATGGCGGGTGTGAAATCTCCAGGGTGG	5	0.125	No Hit
GGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTGACA	5	0.125	No Hit
CCTGCATGCCCACAAACTGCGTGTGGATCCTGTCAACTTCAAGTTCCTGA	5	0.125	No Hit
CGCAGTTTGTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAG	5	0.125	No Hit
GCTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAATTT	5	0.125	No Hit
GGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGC	5	0.125	No Hit
CAAAACCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATGGTGGTG	5	0.125	No Hit
CAGCACAGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGGCGTGCATGG	5	0.125	No Hit
CCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCT	5	0.125	No Hit
TGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTT	5	0.125	No Hit
GAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCA	5	0.125	No Hit
GGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACG	5	0.125	No Hit
GTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGA	5	0.125	No Hit
CACCACCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTGCCC	5	0.125	No Hit
CTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCCTGTCAACTTCAA	5	0.125	No Hit
CAGGATCCACACGCAGTTTGTGGGCATGCAGGTCGCTCAGAGTGGACAGG	5	0.125	No Hit
CCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGAGGTACTTTGAT	5	0.125	No Hit
AGGAAAAGAGGTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCAG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0125	0.0	0.0	0.0	0.0
26-27	0.025	0.0	0.0	0.0	0.0
28-29	0.025	0.0	0.0	0.0	0.0
30-31	0.025	0.0	0.0	0.0	0.0
32-33	0.025	0.0	0.0	0.0	0.0
34-35	0.037500000000000006	0.0	0.0	0.0	0.0
36-37	0.05	0.0	0.0	0.0	0.0
38-39	0.07500000000000001	0.0	0.0	0.0	0.0
40-41	0.1125	0.0	0.0	0.0	0.0
42-43	0.125	0.0	0.0	0.0	0.0
44-45	0.125	0.0	0.0	0.0	0.0
46-47	0.125	0.0	0.0	0.0	0.0
48-49	0.1375	0.0	0.0	0.0	0.0
50-51	0.15	0.0	0.0	0.0	0.0
52-53	0.15	0.0	0.0	0.0	0.0
54-55	0.175	0.0	0.0	0.0	0.0
56-57	0.2	0.0	0.0	0.0	0.0
58-59	0.25	0.0	0.0	0.0	0.0
60-61	0.25	0.0	0.0	0.0	0.0
62-63	0.2875	0.0	0.0	0.0	0.0
64-65	0.3625	0.0	0.0	0.0	0.0
66-67	0.4375	0.0	0.0	0.0	0.0
68-69	0.4625	0.0	0.0	0.0	0.0
70-71	0.5	0.0	0.0	0.0	0.0
72-73	0.5625	0.0	0.0	0.0	0.0
74-75	0.6125	0.0	0.0	0.0	0.0
76-77	0.65	0.0	0.0	0.0	0.0
78-79	0.6875	0.0	0.0	0.0	0.0
80-81	0.7375	0.0	0.0	0.0	0.0
82-83	0.8125	0.0	0.0	0.0	0.0
84-85	0.9375	0.0	0.0	0.0	0.0
86-87	1.0	0.0	0.0	0.0	0.0
88-89	1.1	0.0	0.0	0.0	0.0
90-91	1.2375	0.0	0.0	0.0	0.0
92-93	1.5	0.0	0.0	0.0	0.0
94-95	1.7	0.0	0.0	0.0	0.0
96-97	1.95	0.0	0.0	0.0	0.0
98-99	2.15	0.0	0.0	0.0	0.0
100-101	2.25	0.0	0.0	0.0	0.0
102-103	2.4375	0.0	0.0	0.0	0.0
104-105	2.7375	0.0	0.0	0.0	0.0
106-107	2.9749999999999996	0.0	0.0	0.0	0.0
108-109	3.1375	0.0	0.0	0.0	0.0
110-111	3.4375	0.0	0.0	0.0	0.0
112-113	3.8375	0.0	0.0	0.0	0.0
114-115	4.199999999999999	0.0	0.0	0.0	0.0
116-117	4.5875	0.0	0.0	0.0	0.0
118-119	4.975	0.0	0.0	0.0	0.0
120-121	5.387499999999999	0.0	0.0	0.0	0.0
122-123	5.925000000000001	0.0	0.0	0.0	0.0
124-125	6.4125	0.0	0.0	0.0	0.0
126-127	6.7125	0.0	0.0	0.0	0.0
128-129	7.15	0.0	0.0	0.0	0.0
130-131	7.6625	0.0	0.0	0.0	0.0
132-133	8.287500000000001	0.0	0.0	0.0	0.0
134-135	8.975000000000001	0.0	0.0	0.0	0.0
136-137	9.7375	0.0	0.0	0.0	0.0
138	10.525	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTGAC	10	0.006973645	144.0	5
GTTGACT	10	0.006973645	144.0	6
GTGTTGA	10	0.006973645	144.0	4
GACTCAC	10	0.006973645	144.0	9
GTTGTGT	10	0.006973645	144.0	1
>>END_MODULE
ERR6357085 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR6357085_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.6425	32.0	32.0	32.0	32.0	32.0
2	31.63875	32.0	32.0	32.0	32.0	32.0
3	35.65375	37.0	37.0	37.0	32.0	37.0
4	36.19125	37.0	37.0	37.0	37.0	37.0
5	36.42	37.0	37.0	37.0	37.0	37.0
6	39.94725	41.0	41.0	41.0	37.0	41.0
7	39.9365	41.0	41.0	41.0	37.0	41.0
8	39.916	41.0	41.0	41.0	37.0	41.0
9	40.019	41.0	41.0	41.0	37.0	41.0
10-14	39.9695	41.0	41.0	41.0	37.8	41.0
15-19	39.88355	41.0	41.0	41.0	37.0	41.0
20-24	39.87485	41.0	41.0	41.0	37.0	41.0
25-29	39.73395	41.0	41.0	41.0	37.0	41.0
30-34	39.64574999999999	41.0	41.0	41.0	37.0	41.0
35-39	39.54585000000001	41.0	41.0	41.0	37.0	41.0
40-44	39.47435	41.0	41.0	41.0	37.0	41.0
45-49	39.330549999999995	41.0	41.0	41.0	37.0	41.0
50-54	39.18345	41.0	41.0	41.0	37.0	41.0
55-59	39.068999999999996	41.0	41.0	41.0	37.0	41.0
60-64	38.810050000000004	41.0	41.0	41.0	33.0	41.0
65-69	38.7131	41.0	41.0	41.0	32.0	41.0
70-74	38.58045	41.0	41.0	41.0	32.0	41.0
75-79	38.0921	41.0	39.4	41.0	32.0	41.0
80-84	38.36665000000001	41.0	41.0	41.0	32.0	41.0
85-89	38.1736	41.0	41.0	41.0	32.0	41.0
90-94	37.92765	41.0	41.0	41.0	29.0	41.0
95-99	37.6854	41.0	40.2	41.0	27.0	41.0
100-104	37.50595	41.0	37.0	41.0	27.0	41.0
105-109	37.109449999999995	41.0	37.0	41.0	23.0	41.0
110-114	36.90749999999999	41.0	37.0	41.0	22.0	41.0
115-119	36.571799999999996	41.0	37.0	41.0	22.0	41.0
120-124	36.38125	41.0	37.0	41.0	22.0	41.0
125-129	36.085750000000004	41.0	37.0	41.0	20.0	41.0
130-134	35.66925	41.0	37.0	41.0	14.0	41.0
135-139	35.4527	41.0	36.0	41.0	14.0	41.0
140-144	34.93655	41.0	32.0	41.0	12.0	41.0
145-149	34.564800000000005	41.0	32.0	41.0	12.0	41.0
150	34.48175	41.0	32.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	0.0
17	11.0
18	11.0
19	13.0
20	16.0
21	10.0
22	21.0
23	16.0
24	17.0
25	24.0
26	34.0
27	39.0
28	57.0
29	48.0
30	46.0
31	64.0
32	86.0
33	91.0
34	79.0
35	127.0
36	137.0
37	180.0
38	302.0
39	656.0
40	1913.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.770885442721365	10.78039019509755	7.878939469734868	39.56978489244622
2	32.22416812609457	17.513134851138354	31.77383037277958	18.48886664998749
3	34.06703351675838	21.360680340170084	18.934467233616807	25.63781890945473
4	31.3234926194646	32.67450587940955	14.085564173129846	21.916437327995997
5	31.548661496122094	33.274956217162874	16.787590693019766	18.388791593695274
6	26.36977733299975	34.95121341005754	16.91268451338504	21.766324743557668
7	28.83941970985493	16.58329164582291	30.71535767883942	23.861930965482742
8	24.187093546773387	19.5847923961981	26.038019009504755	30.190095047523762
9	25.99449587190393	19.21441080810608	25.01876407305479	29.772329246935204
10-14	29.62573801661163	23.981787251075755	21.895326728710096	24.497148003602522
15-19	30.19113379365556	22.680876613629543	21.650155108576	25.477834484138896
20-24	29.284034622504628	22.819832891379395	22.544654025116326	25.351478460999648
25-29	29.215450815570897	25.112578805163615	19.66376463524467	26.008205744020813
30-34	27.812249799839872	24.624699759807847	19.855884707766215	27.70716573258607
35-39	28.7887126632311	23.865512583179065	20.648421473958074	26.69735327963176
40-44	27.979585709996996	22.425697988592013	22.10047032923046	27.494245972180526
45-49	28.371278458844134	22.631973980485366	22.476857643232425	26.51988991743808
50-54	27.80807524891179	21.483964576975033	22.989943463251112	27.71801671086206
55-59	27.88091068301226	22.947210407805855	22.481861396047034	26.69001751313485
60-64	28.5156836259943	22.972634949222073	22.692480864475463	25.81920056030817
65-69	27.63434404082858	21.920344240968678	21.605123586510558	28.840188131692184
70-74	28.05463824677274	21.870309216451517	22.255578905233662	27.81947363154208
75-79	28.78514960472331	22.24557190033023	22.30061042729911	26.668668067647356
80-84	27.959571700190132	23.39137396177324	21.8653057139998	26.783748624036825
85-89	29.03177383037278	22.421816362271706	22.636977733299975	25.909432074055545
90-94	27.97878196466997	22.75434119001151	22.08877545914027	27.17810138617825
95-99	28.757254352611568	22.903742245347207	20.912547528517113	27.426455873524112
100-104	28.54498148704093	23.08115680976684	21.32492744921445	27.048934253977784
105-109	29.04533173221255	21.199839887921545	22.450715500850595	27.304112879015314
110-114	28.88588723798089	22.447346040322177	21.937065385962278	26.729701335734653
115-119	28.330581820001	23.332833058182	21.796988343588975	26.539596778228024
120-124	28.470658862374304	23.19775876732203	22.162189204062237	26.169393166241434
125-129	28.04603452589442	23.627720790592946	21.896422316737553	26.42982236677508
130-134	29.043878521038675	22.63971581527993	23.34517436333617	24.971231300345227
135-139	28.24477133993796	24.302011407985592	20.939657760432304	26.513559491644152
140-144	28.796597448086064	23.39254440830623	22.777082812109082	25.033775331498624
145-149	27.878120778506027	24.636013408715666	21.15374993745935	26.332115875318955
150	25.243932949712285	25.243932949712285	21.491118338754063	28.021015761821367
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	0.5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	0.5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	1.0
26	1.0
27	0.5
28	2.0
29	1.5
30	1.0
31	1.5
32	1.0
33	1.0
34	4.0
35	5.0
36	5.5
37	8.5
38	10.5
39	18.0
40	19.5
41	18.0
42	18.5
43	13.5
44	12.5
45	19.5
46	22.0
47	23.0
48	37.0
49	94.5
50	185.5
51	262.5
52	280.0
53	238.5
54	193.5
55	188.5
56	234.5
57	344.0
58	447.0
59	425.0
60	302.5
61	199.5
62	134.0
63	75.0
64	36.5
65	13.5
66	10.0
67	12.5
68	7.5
69	2.5
70	4.0
71	5.5
72	4.5
73	5.0
74	5.5
75	3.5
76	3.5
77	5.0
78	5.5
79	3.5
80	2.5
81	3.0
82	4.0
83	3.0
84	2.5
85	3.0
86	1.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05
2	0.075
3	0.05
4	0.075
5	0.075
6	0.075
7	0.05
8	0.05
9	0.075
10-14	0.06999999999999999
15-19	0.06999999999999999
20-24	0.065
25-29	0.06999999999999999
30-34	0.08
35-39	0.065
40-44	0.06999999999999999
45-49	0.075
50-54	0.065
55-59	0.075
60-64	0.055
65-69	0.06999999999999999
70-74	0.06999999999999999
75-79	0.06999999999999999
80-84	0.06999999999999999
85-89	0.075
90-94	0.08499999999999999
95-99	0.06
100-104	0.06999999999999999
105-109	0.06999999999999999
110-114	0.055
115-119	0.055
120-124	0.055
125-129	0.075
130-134	0.065
135-139	0.06999999999999999
140-144	0.075
145-149	0.065
150	0.075
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.4595852729581	46.35
2	8.717731696995346	10.299999999999999
3	4.697418535759628	8.325000000000001
4	2.708421498095641	6.4
5	1.311891663140076	3.875
6	0.8887008040626322	3.15
7	0.6347862886161659	2.625
8	0.5078290308929327	2.4
9	0.46550994498518833	2.475
>10	1.6081252644942872	14.099999999999998
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCC	31	0.775	No Hit
GTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCT	24	0.6	No Hit
GGAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGG	23	0.575	No Hit
GGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCAC	22	0.5499999999999999	No Hit
GGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACA	22	0.5499999999999999	No Hit
CTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTT	19	0.475	No Hit
CAGGAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAA	19	0.475	No Hit
GGAAAAGAGGTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCAGC	19	0.475	No Hit
GGAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCT	18	0.44999999999999996	No Hit
GTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTGT	18	0.44999999999999996	No Hit
GTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCA	16	0.4	No Hit
CACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGCTGAGAAGG	16	0.4	No Hit
TGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGAC	15	0.375	No Hit
GACAAAACCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATGGTGG	15	0.375	No Hit
GGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACGG	14	0.35000000000000003	No Hit
CTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATGG	14	0.35000000000000003	No Hit
CTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTTCTTGCCG	14	0.35000000000000003	No Hit
AGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATT	14	0.35000000000000003	No Hit
GCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTC	14	0.35000000000000003	No Hit
CTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTG	13	0.325	No Hit
GCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCC	13	0.325	No Hit
CTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTGACAAGCTGCA	13	0.325	No Hit
GGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACG	13	0.325	No Hit
AGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGTC	13	0.325	No Hit
CTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCT	12	0.3	No Hit
GTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGA	12	0.3	No Hit
CTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAA	12	0.3	No Hit
CTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGT	11	0.27499999999999997	No Hit
GTCCACTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCCTGTCA	11	0.27499999999999997	No Hit
GTTGTGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTG	11	0.27499999999999997	No Hit
GGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGG	11	0.27499999999999997	No Hit
GTCAACTTCAAGTTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTTGCCA	11	0.27499999999999997	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	11	0.27499999999999997	Illumina Single End PCR Primer 1 (100% over 50bp)
GCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACAGGATC	10	0.25	No Hit
GGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTG	10	0.25	No Hit
GTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCAT	10	0.25	No Hit
GTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGCT	10	0.25	No Hit
GTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTAT	10	0.25	No Hit
CCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTGCCCAGGTC	9	0.22499999999999998	No Hit
GTGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATG	9	0.22499999999999998	No Hit
CAGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGT	9	0.22499999999999998	No Hit
CCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTTAATGGCCTGTGG	9	0.22499999999999998	No Hit
TGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGAC	9	0.22499999999999998	No Hit
GTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTT	9	0.22499999999999998	No Hit
CGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCT	9	0.22499999999999998	No Hit
GTGCAAGGGAGGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGG	9	0.22499999999999998	No Hit
GCCTGAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAA	9	0.22499999999999998	No Hit
CTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCT	9	0.22499999999999998	No Hit
GAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACTG	9	0.22499999999999998	No Hit
CTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTAT	8	0.2	No Hit
CGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCTGCAGACCACGTCGAAG	8	0.2	No Hit
CCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATGGTGGTGAATAT	8	0.2	No Hit
GGGAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGC	8	0.2	No Hit
GGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGTCG	8	0.2	No Hit
GGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTT	8	0.2	No Hit
GGGGAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGA	8	0.2	No Hit
CTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGGGGAAGGCAGCGAA	8	0.2	No Hit
CTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCTGCTATCATGGGTAACC	8	0.2	No Hit
GGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACT	8	0.2	No Hit
GTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGA	8	0.2	No Hit
CCCGGCTCTGCCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTT	8	0.2	No Hit
CTGACTGATGCTGAGAAGGCTGCTGTTAATGGCCTGTGGGGAAAGGTGAA	7	0.17500000000000002	No Hit
CCTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATG	7	0.17500000000000002	No Hit
CTGAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACT	7	0.17500000000000002	No Hit
CAGGCAGTGGCTCAGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGT	7	0.17500000000000002	No Hit
GGAATTTGTCCAGAGAGGCGTGCATGGCGGGTGTGAAATCTCCAGGGTGG	7	0.17500000000000002	No Hit
CGGGTGTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAG	7	0.17500000000000002	No Hit
CTTGGGAACAATTGACCATTGCACAAAGACAAGAGCAGGAAAAGAGGTTT	7	0.17500000000000002	No Hit
CGCAGTTTGTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAG	7	0.17500000000000002	No Hit
CAAGAAGGTGATAAACGCCTTCAATGATGGCCTGAAACACTTGGACAACC	7	0.17500000000000002	No Hit
GAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCA	7	0.17500000000000002	No Hit
CAGCACAGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGGCGTGCATGG	7	0.17500000000000002	No Hit
CCCATGGCAAGAAGGTGATAAACGCCTTCAATGATGGCCTGAAACACTTG	7	0.17500000000000002	No Hit
CTCACGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCTGCAGACCACGTC	7	0.17500000000000002	No Hit
GTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACC	7	0.17500000000000002	No Hit
CTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCA	7	0.17500000000000002	No Hit
GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATT	6	0.15	No Hit
GGAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACT	6	0.15	No Hit
GTCGAAGACCTGCCTGGTGCCCTGTCCACTCTGAGCGACCTGCATGCCCA	6	0.15	No Hit
AACGCCTTCAATGATGGCCTGAAACACTTGGACAACCTCAAGGGCACCTT	6	0.15	No Hit
GGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACG	6	0.15	No Hit
GTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGG	6	0.15	No Hit
CAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGT	6	0.15	No Hit
CACGCAGTTTGTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGC	6	0.15	No Hit
AGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATC	6	0.15	No Hit
CTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAATTTG	6	0.15	No Hit
GGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTG	6	0.15	No Hit
GGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGC	6	0.15	No Hit
CACAAACTGCGTGTGGATCCTGTCAACTTCAAGTTCCTGAGCCACTGCCT	6	0.15	No Hit
CCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCT	6	0.15	No Hit
CTTGGCCAAAGCTGCAGACCACGTCGAAGACCTGCCTGGTGCCCTGTCCA	6	0.15	No Hit
GCTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAATTT	6	0.15	No Hit
GAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCC	6	0.15	No Hit
CAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACTGCTGG	6	0.15	No Hit
CTCGGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCAT	6	0.15	No Hit
GGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAA	6	0.15	No Hit
GTGATAAACGCCTTCAATGATGGCCTGAAACACTTGGACAACCTCAAGGG	6	0.15	No Hit
CAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTTCT	5	0.125	No Hit
CAGGAGGCGGCTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGC	5	0.125	No Hit
CCACAAACTGCGTGTGGATCCTGTCAACTTCAAGTTCCTGAGCCACTGCC	5	0.125	No Hit
CGAAGACCTGCCTGGTGCCCTGTCCACTCTGAGCGACCTGCATGCCCACA	5	0.125	No Hit
GAACAATTGACCATTGCACAAAGACAAGAGCAGGAAAAGAGGTTTAGTGG	5	0.125	No Hit
CTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCC	5	0.125	No Hit
GGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGG	5	0.125	No Hit
GAGAACTTCAGGCTCCTGGGCAATATGATTGTGATTGTGTTGGGCCACCA	5	0.125	No Hit
CGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCT	5	0.125	No Hit
GCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGAT	5	0.125	No Hit
GGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCC	5	0.125	No Hit
GGAGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATGTTGC	5	0.125	No Hit
CTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCCTGTCAACTTC	5	0.125	No Hit
GGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGAGGTACTTTGATAGCT	5	0.125	No Hit
CTGGTTGTCTACCCTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCT	5	0.125	No Hit
CTTCAATGATGGCCTGAAACACTTGGACAACCTCAAGGGCACCTTTGCTC	5	0.125	No Hit
GTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAG	5	0.125	No Hit
CAGATTTTCAAAGGTCTTTGTCATTTTGACAACAACTGACAGATGCTCTC	5	0.125	No Hit
CCCACCACCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTGC	5	0.125	No Hit
GCGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGAGGTAC	5	0.125	No Hit
GGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATTGAT	5	0.125	No Hit
GTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCAGCCACCACCTT	5	0.125	No Hit
GTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTTCTTGCCGTGAGCCT	5	0.125	No Hit
GTCAGCACAGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGGCGTGCAT	5	0.125	No Hit
CTCAGAGTGGACAGGGCACCAGGCAGGTCTTCAACGTGGTCTGCAGCTTT	5	0.125	No Hit
TGCAGCTTTGGCCAAGGCATCAGCAACCTTCTTGCCGTGAGCCTTGACCT	5	0.125	No Hit
GTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCT	5	0.125	No Hit
GGGACCTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCC	5	0.125	No Hit
GACAGGATCCACACGCAGTTTGTGGGCATGCAGGTCGCTCAGAGTGGACA	5	0.125	No Hit
GGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGC	5	0.125	No Hit
GGGAGGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.025	0.0	0.0	0.0	0.0
26-27	0.05	0.0	0.0	0.0	0.0
28-29	0.05	0.0	0.0	0.0	0.0
30-31	0.05	0.0	0.0	0.0	0.0
32-33	0.05	0.0	0.0	0.0	0.0
34-35	0.0625	0.0	0.0	0.0	0.0
36-37	0.075	0.0	0.0	0.0	0.0
38-39	0.1	0.0	0.0	0.0	0.0
40-41	0.1375	0.0	0.0	0.0	0.0
42-43	0.15	0.0	0.0	0.0	0.0
44-45	0.15	0.0	0.0	0.0	0.0
46-47	0.15	0.0	0.0	0.0	0.0
48-49	0.16249999999999998	0.0	0.0	0.0	0.0
50-51	0.1875	0.0	0.0	0.0	0.0
52-53	0.2	0.0	0.0	0.0	0.0
54-55	0.25	0.0	0.0	0.0	0.0
56-57	0.275	0.0	0.0	0.0	0.0
58-59	0.325	0.0	0.0	0.0	0.0
60-61	0.325	0.0	0.0	0.0	0.0
62-63	0.36250000000000004	0.0	0.0	0.0	0.0
64-65	0.4375	0.0	0.0	0.0	0.0
66-67	0.5125	0.0	0.0	0.0	0.0
68-69	0.5375000000000001	0.0	0.0	0.0	0.0
70-71	0.575	0.0	0.0	0.0	0.0
72-73	0.6375	0.0	0.0	0.0	0.0
74-75	0.6875	0.0	0.0	0.0	0.0
76-77	0.725	0.0	0.0	0.0	0.0
78-79	0.7625	0.0	0.0	0.0	0.0
80-81	0.85	0.0	0.0	0.0	0.0
82-83	0.9125000000000001	0.0	0.0	0.0	0.0
84-85	1.0375	0.0	0.0	0.0	0.0
86-87	1.1	0.0	0.0	0.0	0.0
88-89	1.2	0.0	0.0	0.0	0.0
90-91	1.35	0.0	0.0	0.0	0.0
92-93	1.6375	0.0	0.0	0.0	0.0
94-95	1.85	0.0	0.0	0.0	0.0
96-97	2.075	0.0	0.0	0.0	0.0
98-99	2.275	0.0	0.0	0.0	0.0
100-101	2.375	0.0	0.0	0.0	0.0
102-103	2.5375	0.0	0.0	0.0	0.0
104-105	2.8875	0.0	0.0	0.0	0.0
106-107	3.175	0.0	0.0	0.0	0.0
108-109	3.325	0.0	0.0	0.0	0.0
110-111	3.6	0.0	0.0	0.0	0.0
112-113	3.9875	0.0	0.0	0.0	0.0
114-115	4.375	0.0	0.0	0.0	0.0
116-117	4.7875	0.0	0.0	0.0	0.0
118-119	5.1625	0.0	0.0	0.0	0.0
120-121	5.6	0.0	0.0	0.0	0.0
122-123	6.1375	0.0	0.0	0.0	0.0
124-125	6.6625	0.0	0.0	0.0	0.0
126-127	6.9625	0.0	0.0	0.0	0.0
128-129	7.3875	0.0	0.0	0.0	0.0
130-131	7.8875	0.0	0.0	0.0	0.0
132-133	8.5	0.0	0.0	0.0	0.0
134-135	9.1375	0.0	0.0	0.0	0.0
136-137	9.8625	0.0	0.0	0.0	0.0
138	10.6	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 2544640 spots for ERR6357085.sra
Written 2544640 spots for ERR6357085.sra
Read 2544640 spots for ERR6357085.sra
Written 2544640 spots for ERR6357085.sra
Read 2544640 spots for ERR6357085.sra
Written 2544640 spots for ERR6357085.sra
Read 2544640 spots for ERR6357085.sra
Written 2544640 spots for ERR6357085.sra
Read 2544640 spots for ERR6357085.sra
Written 2544640 spots for ERR6357085.sra
Read 2544640 spots for ERR6357085.sra
Written 2544640 spots for ERR6357085.sra
Read 2544640 spots for ERR6357085.sra
Written 2544640 spots for ERR6357085.sra
Read 2544647 spots for ERR6357085.sra
Written 2544647 spots for ERR6357085.sra
SRR ids: ['ERR6357085.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_bv3gtxvy
ERR6357085.sra spots: 20357127
blocks: [[1, 2544640], [2544641, 5089280], [5089281, 7633920], [7633921, 10178560], [10178561, 12723200], [12723201, 15267840], [15267841, 17812480], [17812481, 20357127]]
ERR6357085 file size 6836912
ERR6357085 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o ERR6357085 ERR6357085_1.fastq ERR6357085_2.fastq
Input file:	ERR6357085_1.fastq
Paired file:	ERR6357085_2.fastq
trimmed:	ERR6357085-trimmed-pair1.fastq, ERR6357085-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Mon Dec 29 01:14:52 2025 >> started

Mon Dec 29 01:15:18 2025 >> done (25.559s)
20357127 read pairs processed; of these:
    1696 ( 0.01%) short read pairs filtered out after trimming by size control
   73501 ( 0.36%) empty read pairs filtered out after trimming by size control
20281930 (99.63%) read pairs available; of these:
 3956927 (19.51%) trimmed read pairs available after processing
16325003 (80.49%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     261	  0.00%
 19	     218	  0.00%
 20	     231	  0.00%
 21	     324	  0.00%
 22	     332	  0.00%
 23	     389	  0.00%
 24	     471	  0.00%
 25	     512	  0.00%
 26	     579	  0.00%
 27	     666	  0.00%
 28	     728	  0.00%
 29	     745	  0.00%
 30	     860	  0.00%
 31	     919	  0.00%
 32	     921	  0.00%
 33	    1082	  0.01%
 34	    1021	  0.01%
 35	    1157	  0.01%
 36	    1334	  0.01%
 37	    1327	  0.01%
 38	    1574	  0.01%
 39	    1430	  0.01%
 40	    1621	  0.01%
 41	    1881	  0.01%
 42	    1978	  0.01%
 43	    2161	  0.01%
 44	    2080	  0.01%
 45	    2071	  0.01%
 46	    2363	  0.01%
 47	    2356	  0.01%
 48	    2519	  0.01%
 49	    3000	  0.01%
 50	    2928	  0.01%
 51	    3328	  0.02%
 52	    3329	  0.02%
 53	    3345	  0.02%
 54	    3332	  0.02%
 55	    3239	  0.02%
 56	    3451	  0.02%
 57	    4024	  0.02%
 58	    3829	  0.02%
 59	    4347	  0.02%
 60	    3931	  0.02%
 61	    4578	  0.02%
 62	    4551	  0.02%
 63	    4789	  0.02%
 64	    4922	  0.02%
 65	    4986	  0.02%
 66	    5810	  0.03%
 67	    5686	  0.03%
 68	    6728	  0.03%
 69	    5843	  0.03%
 70	    6522	  0.03%
 71	    6644	  0.03%
 72	    7045	  0.03%
 73	    7864	  0.04%
 74	    7287	  0.04%
 75	    7301	  0.04%
 76	    7449	  0.04%
 77	    7401	  0.04%
 78	    8332	  0.04%
 79	    8643	  0.04%
 80	    8718	  0.04%
 81	    9271	  0.05%
 82	   10839	  0.05%
 83	   11096	  0.05%
 84	   11392	  0.06%
 85	   11299	  0.06%
 86	   11938	  0.06%
 87	   12651	  0.06%
 88	   13242	  0.07%
 89	   14599	  0.07%
 90	   14681	  0.07%
 91	   15135	  0.07%
 92	   15717	  0.08%
 93	   18371	  0.09%
 94	   20062	  0.10%
 95	   21077	  0.10%
 96	   20782	  0.10%
 97	   18699	  0.09%
 98	   20216	  0.10%
 99	   20739	  0.10%
100	   21682	  0.11%
101	   21245	  0.10%
102	   23262	  0.11%
103	   28392	  0.14%
104	   29196	  0.14%
105	   32866	  0.16%
106	   27425	  0.14%
107	   30877	  0.15%
108	   30422	  0.15%
109	   34668	  0.17%
110	   34749	  0.17%
111	   35011	  0.17%
112	   33413	  0.16%
113	   36362	  0.18%
114	   42925	  0.21%
115	   43905	  0.22%
116	   41047	  0.20%
117	   43943	  0.22%
118	   41485	  0.20%
119	   42690	  0.21%
120	   39988	  0.20%
121	   45953	  0.23%
122	   45144	  0.22%
123	   50092	  0.25%
124	   53321	  0.26%
125	   54013	  0.27%
126	   56418	  0.28%
127	   51361	  0.25%
128	   65089	  0.32%
129	   51739	  0.26%
130	   59457	  0.29%
131	   62505	  0.31%
132	   68678	  0.34%
133	   63137	  0.31%
134	   67052	  0.33%
135	   80172	  0.40%
136	   78612	  0.39%
137	   78952	  0.39%
138	   81202	  0.40%
139	   77944	  0.38%
140	   76169	  0.38%
141	   80663	  0.40%
142	   88863	  0.44%
143	   79714	  0.39%
144	   98382	  0.49%
145	   92365	  0.46%
146	  110708	  0.55%
147	  123996	  0.61%
148	  168864	  0.83%
149	  629710	  3.10%
150	16325003	 80.49%
20281930 reads passed initial QC


criterion=sequence-density
sequence-density=5.13
sequence-density-rank=1
fanout-score=2.36
fanout-score-rank=43
prefix-density=5.05
prefix-fanout=2.4
sequence=GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA


criterion=fanout-score
sequence-density=0.61
sequence-density-rank=18
fanout-score=30.47
fanout-score-rank=1
prefix-density=9.31
prefix-fanout=2.0
sequence=GCAAGGAATTCTCCCCCTGTGCA


criterion=sequence-density
sequence-density=4.97
sequence-density-rank=1
fanout-score=2.38
fanout-score-rank=41
prefix-density=4.89
prefix-fanout=2.4
sequence=GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA


criterion=fanout-score
sequence-density=0.62
sequence-density-rank=18
fanout-score=29.61
fanout-score-rank=1
prefix-density=9.06
prefix-fanout=2.0
sequence=GCAAGGAATTCTCCCCCTGTGCA
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA -y GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA -o ERR6357085 ERR6357085_1.fastq ERR6357085_2.fastq
Input file:	ERR6357085_1.fastq
Paired file:	ERR6357085_2.fastq
trimmed:	ERR6357085-trimmed-pair1.fastq, ERR6357085-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGA
-- paired 3' end adapter sequence (-y):	GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Mon Dec 29 01:16:17 2025 >> started

Mon Dec 29 01:16:42 2025 >> done (24.231s)
13521287 read pairs processed; of these:
    9199 ( 0.07%) short read pairs filtered out after trimming by size control
  120997 ( 0.89%) empty read pairs filtered out after trimming by size control
13391091 (99.04%) read pairs available; of these:
    1906 ( 0.01%) trimmed read pairs available after processing
13389185 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     174	  0.00%
 19	     146	  0.00%
 20	     169	  0.00%
 21	     216	  0.00%
 22	     216	  0.00%
 23	     266	  0.00%
 24	     318	  0.00%
 25	     329	  0.00%
 26	     389	  0.00%
 27	     422	  0.00%
 28	     475	  0.00%
 29	     487	  0.00%
 30	     569	  0.00%
 31	     629	  0.00%
 32	     645	  0.00%
 33	     703	  0.01%
 34	     661	  0.00%
 35	     755	  0.01%
 36	     900	  0.01%
 37	     863	  0.01%
 38	    1032	  0.01%
 39	     955	  0.01%
 40	    1071	  0.01%
 41	    1224	  0.01%
 42	    1308	  0.01%
 43	    1449	  0.01%
 44	    1383	  0.01%
 45	    1389	  0.01%
 46	    1566	  0.01%
 47	    1547	  0.01%
 48	    1673	  0.01%
 49	    1960	  0.01%
 50	    1925	  0.01%
 51	    2210	  0.02%
 52	    2191	  0.02%
 53	    2167	  0.02%
 54	    2117	  0.02%
 55	    2116	  0.02%
 56	    2218	  0.02%
 57	    2694	  0.02%
 58	    2570	  0.02%
 59	    2853	  0.02%
 60	    2608	  0.02%
 61	    3086	  0.02%
 62	    3049	  0.02%
 63	    3202	  0.02%
 64	    3242	  0.02%
 65	    3295	  0.02%
 66	    3886	  0.03%
 67	    3826	  0.03%
 68	    4463	  0.03%
 69	    3798	  0.03%
 70	    4331	  0.03%
 71	    4350	  0.03%
 72	    4639	  0.03%
 73	    5164	  0.04%
 74	    4724	  0.04%
 75	    4861	  0.04%
 76	    4887	  0.04%
 77	    4941	  0.04%
 78	    5461	  0.04%
 79	    5652	  0.04%
 80	    5740	  0.04%
 81	    6121	  0.05%
 82	    7220	  0.05%
 83	    7333	  0.05%
 84	    7591	  0.06%
 85	    7469	  0.06%
 86	    7969	  0.06%
 87	    8510	  0.06%
 88	    8741	  0.07%
 89	    9711	  0.07%
 90	    9781	  0.07%
 91	   10032	  0.07%
 92	   10371	  0.08%
 93	   12144	  0.09%
 94	   13404	  0.10%
 95	   13983	  0.10%
 96	   13850	  0.10%
 97	   12387	  0.09%
 98	   13413	  0.10%
 99	   13657	  0.10%
100	   14345	  0.11%
101	   14103	  0.11%
102	   15384	  0.11%
103	   18963	  0.14%
104	   19404	  0.14%
105	   21745	  0.16%
106	   18212	  0.14%
107	   20508	  0.15%
108	   20328	  0.15%
109	   22932	  0.17%
110	   23093	  0.17%
111	   23380	  0.17%
112	   22167	  0.17%
113	   24238	  0.18%
114	   28473	  0.21%
115	   29146	  0.22%
116	   27279	  0.20%
117	   29211	  0.22%
118	   27559	  0.21%
119	   28261	  0.21%
120	   26514	  0.20%
121	   30557	  0.23%
122	   29782	  0.22%
123	   33195	  0.25%
124	   35409	  0.26%
125	   35814	  0.27%
126	   37302	  0.28%
127	   34078	  0.25%
128	   43339	  0.32%
129	   33950	  0.25%
130	   38942	  0.29%
131	   40915	  0.31%
132	   45390	  0.34%
133	   42027	  0.31%
134	   44448	  0.33%
135	   53049	  0.40%
136	   51874	  0.39%
137	   51806	  0.39%
138	   53247	  0.40%
139	   50965	  0.38%
140	   49950	  0.37%
141	   52535	  0.39%
142	   58713	  0.44%
143	   52827	  0.39%
144	   65069	  0.49%
145	   60463	  0.45%
146	   72557	  0.54%
147	   80936	  0.60%
148	  111311	  0.83%
149	  415440	  3.10%
150	10778114	 80.49%


criterion=sequence-density
sequence-density=5.06
sequence-density-rank=1
fanout-score=2.37
fanout-score-rank=42
prefix-density=5.10
prefix-fanout=2.4
sequence=GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA


criterion=fanout-score
sequence-density=0.58
sequence-density-rank=19
fanout-score=31.80
fanout-score-rank=1
prefix-density=9.34
prefix-fanout=2.0
sequence=GCAAGGAATTCTCCCCCTGTGCA


criterion=sequence-density
sequence-density=4.91
sequence-density-rank=1
fanout-score=2.40
fanout-score-rank=42
prefix-density=4.96
prefix-fanout=2.4
sequence=GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA


criterion=fanout-score
sequence-density=0.60
sequence-density-rank=18
fanout-score=30.10
fanout-score-rank=1
prefix-density=9.04
prefix-fanout=2.0
sequence=GCAAGGAATTCTCCCCCTGTGCA
ERR6357085 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Dec 29 01:17:19
                             Started mapping on |	Dec 29 01:17:19
                                    Finished on |	Dec 29 01:26:20
       Mapping speed, Million of reads per hour |	134.10

                          Number of input reads |	20151734
                      Average input read length |	290
                                    UNIQUE READS:
                   Uniquely mapped reads number |	15586399
                        Uniquely mapped reads % |	77.35%
                          Average mapped length |	289.34
                       Number of splices: Total |	20000493
            Number of splices: Annotated (sjdb) |	19611915
                       Number of splices: GT/AG |	19909970
                       Number of splices: GC/AG |	21314
                       Number of splices: AT/AC |	1652
               Number of splices: Non-canonical |	67557
                      Mismatch rate per base, % |	0.53%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.99
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.76
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	3924034
             % of reads mapped to multiple loci |	19.47%
        Number of reads mapped to too many loci |	45651
             % of reads mapped to too many loci |	0.23%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	2.93%
                     % of reads unmapped: other |	0.02%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	641301	641301	641301
N_multimapping	3924034	3924034	3924034
N_noFeature	302528	7862545	7956648
N_ambiguous	283452	104167	109803
UnstrandedReadsAssigned:15000419 PositiveStrandReadsAssigned:7619687 NegativeStrandReadsAssigned:7519948
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
ERR6357085 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 31,715
[index] number of k-mers: 49,915,185
[index] number of equivalence classes: 70,351
[quant] running in paired-end mode
[quant] will process pair 1: ERR6357085-trimmed-pair1.fastq
                             ERR6357085-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 20,151,734 reads, 16,341,105 reads pseudoaligned
[quant] estimated average fragment length: 200.088
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 840 rounds

  31716 ERR6357085.ke.tsv
  32883 ERR6357085.se.tsv
  64599 total
==> ERR6357085.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	755.912	1947	67.0575
ENSRNOT00000040993.4	1039	839.912	1484	45.9995
ENSRNOT00000050156.3	1545	1345.91	15210	294.215
ENSRNOT00000043693.3	684	485.065	4353	233.637
ENSRNOT00000046201.3	204	48.3535	131	70.5336
ENSRNOT00000046108.3	681	482.065	4552	245.839
ENSRNOT00000049683.3	784	584.97	7619	339.092
ENSRNOT00000041241.3	348	151.662	221	37.9375
ENSRNOT00000044582.3	297	106.955	89	21.6641

==> ERR6357085.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	0
ENSRNOG00000058808	0
ENSRNOG00000061316	4
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	0
ERR6357085 completed mapping pipeline successfully
