Starting /dee2/code/volunteer_pipeline.sh ERR6357090
    current disk space = 190870576
    free memory = 195183104 
ERR6357090 SRAfilesize
e50ebada27783c7544a7ab0045da64fe  ERR6357090.sra
ERR6357090.sra file validated
ERR6357090 is paired end
ERR6357090 is conventional basespace
ERR6357090 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR6357090_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.52125	32.0	32.0	32.0	32.0	32.0
2	31.4725	32.0	32.0	32.0	32.0	32.0
3	35.4075	37.0	37.0	37.0	32.0	37.0
4	36.035	37.0	37.0	37.0	32.0	37.0
5	36.48625	37.0	37.0	37.0	37.0	37.0
6	39.946	41.0	41.0	41.0	37.0	41.0
7	39.9795	41.0	41.0	41.0	37.0	41.0
8	40.12875	41.0	41.0	41.0	37.0	41.0
9	40.1765	41.0	41.0	41.0	37.0	41.0
10-14	40.1868	41.0	41.0	41.0	37.0	41.0
15-19	40.0166	41.0	41.0	41.0	37.0	41.0
20-24	39.8133	41.0	41.0	41.0	37.0	41.0
25-29	39.549299999999995	41.0	41.0	41.0	37.0	41.0
30-34	39.198699999999995	41.0	41.0	41.0	37.0	41.0
35-39	39.0192	41.0	41.0	41.0	37.0	41.0
40-44	38.80675	41.0	41.0	41.0	34.0	41.0
45-49	38.54565	41.0	39.4	41.0	32.0	41.0
50-54	38.22975	41.0	37.8	41.0	32.0	41.0
55-59	38.1266	41.0	37.0	41.0	32.0	41.0
60-64	37.767700000000005	41.0	37.0	41.0	27.0	41.0
65-69	37.3718	41.0	37.0	41.0	27.0	41.0
70-74	36.965700000000005	41.0	37.0	41.0	27.0	41.0
75-79	36.98845	41.0	37.0	41.0	27.0	41.0
80-84	37.3113	41.0	37.0	41.0	27.0	41.0
85-89	37.10510000000001	41.0	37.0	41.0	26.0	41.0
90-94	36.757400000000004	41.0	37.0	41.0	23.0	41.0
95-99	36.313900000000004	41.0	37.0	41.0	22.0	41.0
100-104	35.87405	41.0	35.0	41.0	22.0	41.0
105-109	35.638149999999996	41.0	32.0	41.0	22.0	41.0
110-114	35.297700000000006	41.0	32.0	41.0	22.0	41.0
115-119	34.82005	41.0	32.0	41.0	16.0	41.0
120-124	34.426550000000006	41.0	32.0	41.0	12.0	41.0
125-129	33.919500000000006	37.8	31.0	41.0	12.0	41.0
130-134	33.46985	37.0	28.0	41.0	12.0	41.0
135-139	32.8256	37.0	26.0	41.0	12.0	41.0
140-144	32.4917	37.0	26.0	41.0	12.0	41.0
145-149	31.97275	37.0	22.0	41.0	12.0	41.0
150	31.591	37.0	22.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	1.0
19	6.0
20	2.0
21	15.0
22	16.0
23	22.0
24	30.0
25	39.0
26	51.0
27	42.0
28	72.0
29	62.0
30	64.0
31	84.0
32	121.0
33	125.0
34	179.0
35	249.0
36	323.0
37	501.0
38	579.0
39	618.0
40	798.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.78519629907477	10.702675668917228	7.801950487621906	40.71017754438609
2	33.275	18.05	30.525000000000002	18.15
3	34.275	21.0	18.525	26.200000000000003
4	32.2	32.300000000000004	13.925	21.575
5	32.025	33.7	16.7	17.575
6	26.375	35.675000000000004	14.825	23.125
7	29.075	17.224999999999998	29.225	24.474999999999998
8	24.025	19.55	25.650000000000002	30.775000000000002
9	27.125	19.0	27.400000000000002	26.474999999999998
10-14	29.68	23.73	22.005	24.585
15-19	30.14	22.31	21.72	25.83
20-24	29.585	21.92	22.884999999999998	25.61
25-29	29.425	24.79	19.68	26.105
30-34	28.845	24.52	19.59	27.045
35-39	28.985	23.815	20.24	26.96
40-44	27.900000000000002	22.045	22.425	27.63
45-49	28.221411070553525	22.846142307115354	22.3911195559778	26.541327066353315
50-54	27.894999999999996	21.94	22.575	27.589999999999996
55-59	28.88144407220361	22.046102305115255	21.996099804990248	27.076353817690883
60-64	28.77	21.985	22.56	26.685
65-69	28.081404070203508	21.516075803790187	21.75608780439022	28.646432321616082
70-74	28.375	22.49	21.14	27.994999999999997
75-79	28.904999999999998	21.665	22.009999999999998	27.42
80-84	27.502750275027505	23.187318731873187	22.622262226222624	26.687668766876687
85-89	28.725	21.48	22.34	27.455000000000002
90-94	27.996399819990998	22.601130056502825	21.976098804940246	27.426371318565927
95-99	28.29	22.615	21.23	27.865000000000002
100-104	27.92639631981599	22.97614880744037	21.651082554127708	27.44637231861593
105-109	28.84	21.22	22.009999999999998	27.93
110-114	28.7	22.39	21.349999999999998	27.560000000000002
115-119	27.474999999999998	23.380000000000003	21.67	27.474999999999998
120-124	27.61	22.75	21.895	27.744999999999997
125-129	26.735	23.400000000000002	21.740000000000002	28.125
130-134	28.405	22.93	22.445	26.22
135-139	26.44	24.925	20.405	28.23
140-144	27.3	22.365	22.98	27.355
145-149	26.47	24.75	20.69	28.09
150	22.3	26.075	20.65	30.975
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	0.5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	1.0
31	2.0
32	1.5
33	1.5
34	1.5
35	2.5
36	6.5
37	10.5
38	9.0
39	8.0
40	11.0
41	12.5
42	10.0
43	8.5
44	15.5
45	18.5
46	19.0
47	23.0
48	36.5
49	88.5
50	173.0
51	226.5
52	236.0
53	221.0
54	199.5
55	186.5
56	234.0
57	398.5
58	499.0
59	448.5
60	325.5
61	207.5
62	130.0
63	70.0
64	44.0
65	25.5
66	14.0
67	9.0
68	5.5
69	7.5
70	6.0
71	3.0
72	2.0
73	1.5
74	2.0
75	2.5
76	1.0
77	1.5
78	2.5
79	4.0
80	5.5
81	3.5
82	3.0
83	2.0
84	1.0
85	3.5
86	4.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.005
50-54	0.0
55-59	0.005
60-64	0.0
65-69	0.005
70-74	0.0
75-79	0.0
80-84	0.01
85-89	0.0
90-94	0.005
95-99	0.0
100-104	0.005
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.15
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.93200362647326	41.325
2	10.652765185856754	11.75
3	4.895738893925658	8.1
4	2.674524025385313	5.8999999999999995
5	1.5865820489573892	4.375
6	1.314596554850408	4.35
7	0.5893019038984587	2.275
8	0.5893019038984587	2.6
9	0.7252946509519492	3.5999999999999996
>10	2.0398912058023573	15.725
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGG	32	0.8	No Hit
GTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACC	23	0.575	No Hit
GTCAACTTCAAGTTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTTGCCA	21	0.525	No Hit
GGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCAC	19	0.475	No Hit
GGAAAAGAGGTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCAGC	19	0.475	No Hit
GTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCT	18	0.44999999999999996	No Hit
GACAAAACCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATGGTGG	17	0.42500000000000004	No Hit
GTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCC	17	0.42500000000000004	No Hit
GGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGG	17	0.42500000000000004	No Hit
CTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCT	16	0.4	No Hit
GGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACA	16	0.4	No Hit
GTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCA	15	0.375	No Hit
TGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGAC	15	0.375	No Hit
CTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTT	15	0.375	No Hit
CCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCT	15	0.375	No Hit
CAGGAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAA	15	0.375	No Hit
CCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTGCCCAGGTC	14	0.35000000000000003	No Hit
GGAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACT	14	0.35000000000000003	No Hit
CGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCTGCAGACCACGTCGAAG	14	0.35000000000000003	No Hit
GTGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATG	14	0.35000000000000003	No Hit
GCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTC	14	0.35000000000000003	No Hit
GCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTG	14	0.35000000000000003	No Hit
GGAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCT	14	0.35000000000000003	No Hit
CTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTG	13	0.325	No Hit
CACAAACTGCGTGTGGATCCTGTCAACTTCAAGTTCCTGAGCCACTGCCT	13	0.325	No Hit
CTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTGACAAGCTGCA	13	0.325	No Hit
CGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCT	13	0.325	No Hit
AGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATT	12	0.3	No Hit
CCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCTGCTATC	12	0.3	No Hit
GGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTG	11	0.27499999999999997	No Hit
GCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCC	11	0.27499999999999997	No Hit
CTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCCTGTCAACTTC	11	0.27499999999999997	No Hit
GGGGAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGA	11	0.27499999999999997	No Hit
GTGCAAGGGAGGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGG	11	0.27499999999999997	No Hit
CTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGT	10	0.25	No Hit
CCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATGGTGGTGAATAT	10	0.25	No Hit
CTGGGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGTAGGTCTTGGTGG	10	0.25	No Hit
CACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGCTGAGAAGG	10	0.25	No Hit
CAGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGT	10	0.25	No Hit
CAGGCAGTGGCTCAGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGT	10	0.25	No Hit
CGGGTGTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAG	10	0.25	No Hit
GGGAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGC	10	0.25	No Hit
GGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTT	10	0.25	No Hit
GGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACG	10	0.25	No Hit
GTCAGCACAGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGGCGTGCAT	10	0.25	No Hit
GGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACGG	9	0.22499999999999998	No Hit
GTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGCT	9	0.22499999999999998	No Hit
CAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACAT	9	0.22499999999999998	No Hit
CCTGTCCACTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCCTG	9	0.22499999999999998	No Hit
GAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTG	9	0.22499999999999998	No Hit
AAAAGAGGTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCAGCCA	9	0.22499999999999998	No Hit
GAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACGGT	9	0.22499999999999998	No Hit
CTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTTCTTGCCG	9	0.22499999999999998	No Hit
GTCCACTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCCTGTCA	9	0.22499999999999998	No Hit
GTTGTGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTG	9	0.22499999999999998	No Hit
CAAGAAGGTGATAAACGCCTTCAATGATGGCCTGAAACACTTGGACAACC	9	0.22499999999999998	No Hit
GTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCAGCCACCACCTT	9	0.22499999999999998	No Hit
GGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACT	9	0.22499999999999998	No Hit
GGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGC	9	0.22499999999999998	No Hit
CAGGAAAAGAGGTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCA	9	0.22499999999999998	No Hit
GTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTGT	9	0.22499999999999998	No Hit
CAGGAGGCGGCTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGC	8	0.2	No Hit
CTGCAGATGACAAAACCAACATCAAGAACTGCTGGGGGAAGATTGGTGGC	8	0.2	No Hit
GCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACAGGATC	8	0.2	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC	8	0.2	TruSeq Adapter, Index 7 (100% over 50bp)
GTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTTCTTGC	8	0.2	No Hit
CGAAGACCTGCCTGGTGCCCTGTCCACTCTGAGCGACCTGCATGCCCACA	8	0.2	No Hit
CCTTGACCTGGGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGTAGGTC	8	0.2	No Hit
GGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACG	8	0.2	No Hit
CTGAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACT	8	0.2	No Hit
CTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCTGCTATCATGGGTAACC	8	0.2	No Hit
CTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCT	8	0.2	No Hit
GAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACTG	8	0.2	No Hit
CAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCAT	8	0.2	No Hit
CTGCTGGTTGTCTACCCTTGGACCCAGAGGTACTTTGATAGCTTTGGGGA	7	0.17500000000000002	No Hit
CTCACATTGATGTAAGCCCCGGCTCTGCCCAGGTCAAGGCTCACGGCAAG	7	0.17500000000000002	No Hit
CTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGG	7	0.17500000000000002	No Hit
AAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACTGC	7	0.17500000000000002	No Hit
ATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGT	7	0.17500000000000002	No Hit
CCTGCATGCCCACAAACTGCGTGTGGATCCTGTCAACTTCAAGTTCCTGA	7	0.17500000000000002	No Hit
GGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAGGG	7	0.17500000000000002	No Hit
GAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCC	7	0.17500000000000002	No Hit
CTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGGGGAAGGCAGCGAA	7	0.17500000000000002	No Hit
CTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTCCTGGG	7	0.17500000000000002	No Hit
AGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGTC	7	0.17500000000000002	No Hit
GTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCT	7	0.17500000000000002	No Hit
CCCGGCTCTGCCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTT	7	0.17500000000000002	No Hit
CTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTAT	6	0.15	No Hit
GGGGACCTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGC	6	0.15	No Hit
CGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAATTTGTCCAG	6	0.15	No Hit
CACAAAGACAAGAGCAGGAAAAGAGGTTTAGTGGTACTTGTGAGCCAGGG	6	0.15	No Hit
GTCGAAGACCTGCCTGGTGCCCTGTCCACTCTGAGCGACCTGCATGCCCA	6	0.15	No Hit
CCCCACCACCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTG	6	0.15	No Hit
CTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATGG	6	0.15	No Hit
GTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCAT	6	0.15	No Hit
GTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGG	6	0.15	No Hit
GTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTC	6	0.15	No Hit
AAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCA	6	0.15	No Hit
GACAGATTTTCAAAGGTCTTTGTCATTTTGACAACAACTGACAGATGCTC	6	0.15	No Hit
CGCAGTTTGTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAG	6	0.15	No Hit
CCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTTAATGGCCTGTGG	6	0.15	No Hit
AAGGGAGGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGC	6	0.15	No Hit
CTTGGCCAAAGCTGCAGACCACGTCGAAGACCTGCCTGGTGCCCTGTCCA	6	0.15	No Hit
GGGCACCTTTGCTCATCTGAGTGAACTCCACTGTGACAAGCTGCATGTGG	6	0.15	No Hit
CTGCAGACCACGTCGAAGACCTGCCTGGTGCCCTGTCCACTCTGAGCGAC	6	0.15	No Hit
TGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTT	6	0.15	No Hit
GTTGACTCGCAACCTCAGGAACAGACACCATGGTGCACCTAACTGATGCT	6	0.15	No Hit
CTCGGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCAT	6	0.15	No Hit
CTCTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATGGCAAGAAGGTGA	6	0.15	No Hit
CTGATGCCTTGGCCAAAGCTGCAGACCACGTCGAAGACCTGCCTGGTGCC	6	0.15	No Hit
GTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTTCTTGCCGTGAGCCT	6	0.15	No Hit
GTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGA	6	0.15	No Hit
GCCCTGTCCACTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCC	6	0.15	No Hit
CTCACGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCTGCAGACCACGTC	6	0.15	No Hit
CTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCA	6	0.15	No Hit
GTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGA	6	0.15	No Hit
CGGCAAGAAGGTTGCTGATGCCCTGGCCAAAGCTGCAGACCACGTCGAAG	5	0.125	No Hit
CCTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATG	5	0.125	No Hit
GTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATTGATGTAA	5	0.125	No Hit
CTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATGGCAAGAAGGTGATA	5	0.125	No Hit
GGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGT	5	0.125	No Hit
CAGCAACCTTCTTGCCGTGAGCCTTGACCTGGGCAGAGCCGGGGCTTACA	5	0.125	No Hit
AGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACGGTAC	5	0.125	No Hit
CACGCAGTTTGTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGC	5	0.125	No Hit
GCTGGTTGTCTACCCTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACC	5	0.125	No Hit
CTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAATTTG	5	0.125	No Hit
GCGGGTGTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCA	5	0.125	No Hit
CGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCT	5	0.125	No Hit
CATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGG	5	0.125	No Hit
GCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGTCT	5	0.125	No Hit
GGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTGACA	5	0.125	No Hit
GAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGA	5	0.125	No Hit
ACAGGATCCACACGCAGTTTGTGGGCATGCAGGTCGCTCAGAGTGGACAG	5	0.125	No Hit
GTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCTGCAGA	5	0.125	No Hit
GGAGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATGTTGC	5	0.125	No Hit
AATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACC	5	0.125	No Hit
GGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGAGGTACTTTGATAGCT	5	0.125	No Hit
GTGAAAGTAAATGCCTTTTATTAGTAGGACAGATTTTCAAAGGTCTTTGT	5	0.125	No Hit
CTGGTTGTCTACCCTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCT	5	0.125	No Hit
GGCTGCTGTTAATGGCCTGTGGGGAAAGGTGAACCCTGATGATGTTGGTG	5	0.125	No Hit
GAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCA	5	0.125	No Hit
CTGTTAATGGCCTGTGGGGAAAGGTGAACCCTGATGATGTTGGTGGCGAG	5	0.125	No Hit
CCCACCACCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTGC	5	0.125	No Hit
CCGGCTCTGCCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTTG	5	0.125	No Hit
GTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTAT	5	0.125	No Hit
GCCTGAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAA	5	0.125	No Hit
GGGTAACCCTAAGGTGAAGGCCCATGGCAAGAAGGTGATAAACGCCTTCA	5	0.125	No Hit
CAGACACCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTTAATGGC	5	0.125	No Hit
CTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAA	5	0.125	No Hit
GGGAGGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTT	5	0.125	No Hit
CTACCCTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTCTG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.025	0.0	0.0	0.0	0.0
18-19	0.025	0.0	0.0	0.0	0.0
20-21	0.025	0.0	0.0	0.0	0.0
22-23	0.037500000000000006	0.0	0.0	0.0	0.0
24-25	0.05	0.0	0.0	0.0	0.0
26-27	0.05	0.0	0.0	0.0	0.0
28-29	0.05	0.0	0.0	0.0	0.0
30-31	0.05	0.0	0.0	0.0	0.0
32-33	0.05	0.0	0.0	0.0	0.0
34-35	0.1	0.0	0.0	0.0	0.0
36-37	0.15	0.0	0.0	0.0	0.0
38-39	0.16249999999999998	0.0	0.0	0.0	0.0
40-41	0.175	0.0	0.0	0.0	0.0
42-43	0.175	0.0	0.0	0.0	0.0
44-45	0.225	0.0	0.0	0.0	0.0
46-47	0.2625	0.0	0.0	0.0	0.0
48-49	0.2875	0.0	0.0	0.0	0.0
50-51	0.3	0.0	0.0	0.0	0.0
52-53	0.3625	0.0	0.0	0.0	0.0
54-55	0.4	0.0	0.0	0.0	0.0
56-57	0.4	0.0	0.0	0.0	0.0
58-59	0.4	0.0	0.0	0.0	0.0
60-61	0.4	0.0	0.0	0.0	0.0
62-63	0.4375	0.0	0.0	0.0	0.0
64-65	0.5375000000000001	0.0	0.0	0.0	0.0
66-67	0.575	0.0	0.0	0.0	0.0
68-69	0.6	0.0	0.0	0.0	0.0
70-71	0.65	0.0	0.0	0.0	0.0
72-73	0.725	0.0	0.0	0.0	0.0
74-75	0.775	0.0	0.0	0.0	0.0
76-77	0.85	0.0	0.0	0.0	0.0
78-79	0.9	0.0	0.0	0.0	0.0
80-81	0.975	0.0	0.0	0.0	0.0
82-83	1.1625	0.0	0.0	0.0	0.0
84-85	1.325	0.0	0.0	0.0	0.0
86-87	1.425	0.0	0.0	0.0	0.0
88-89	1.475	0.0	0.0	0.0	0.0
90-91	1.6375000000000002	0.0	0.0	0.0	0.0
92-93	1.7875	0.0	0.0	0.0	0.0
94-95	1.9125	0.0	0.0	0.0	0.0
96-97	2.2125	0.0	0.0	0.0	0.0
98-99	2.5125	0.0	0.0	0.0	0.0
100-101	2.8	0.0	0.0	0.0	0.0
102-103	3.2125	0.0	0.0	0.0	0.0
104-105	3.425	0.0	0.0	0.0	0.0
106-107	3.7249999999999996	0.0	0.0	0.0	0.0
108-109	4.0625	0.0	0.0	0.0	0.0
110-111	4.449999999999999	0.0	0.0	0.0	0.0
112-113	4.95	0.0	0.0	0.0	0.0
114-115	5.3375	0.0	0.0	0.0	0.0
116-117	5.9	0.0	0.0	0.0	0.0
118-119	6.4625	0.0	0.0	0.0	0.0
120-121	6.9	0.0	0.0	0.0	0.0
122-123	7.3625	0.0	0.0	0.0	0.0
124-125	8.0625	0.0	0.0	0.0	0.0
126-127	8.825	0.0	0.0	0.0	0.0
128-129	9.5125	0.0	0.0	0.0	0.0
130-131	10.1125	0.0	0.0	0.0	0.0
132-133	10.7375	0.0	0.0	0.0	0.0
134-135	11.575	0.0	0.0	0.0	0.0
136-137	12.6875	0.0	0.0	0.0	0.0
138	13.325	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGAGCAC	20	0.006139246	28.8	140-144
>>END_MODULE
ERR6357090 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR6357090_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71375	32.0	32.0	32.0	32.0	32.0
2	31.6325	32.0	32.0	32.0	32.0	32.0
3	35.6975	37.0	37.0	37.0	32.0	37.0
4	36.16875	37.0	37.0	37.0	37.0	37.0
5	36.37	37.0	37.0	37.0	37.0	37.0
6	39.9325	41.0	41.0	41.0	37.0	41.0
7	40.01575	41.0	41.0	41.0	37.0	41.0
8	40.0085	41.0	41.0	41.0	37.0	41.0
9	40.10475	41.0	41.0	41.0	41.0	41.0
10-14	40.13205	41.0	41.0	41.0	41.0	41.0
15-19	39.96490000000001	41.0	41.0	41.0	37.0	41.0
20-24	39.916999999999994	41.0	41.0	41.0	37.0	41.0
25-29	39.81545	41.0	41.0	41.0	37.0	41.0
30-34	39.68105	41.0	41.0	41.0	37.0	41.0
35-39	39.5947	41.0	41.0	41.0	37.0	41.0
40-44	39.5456	41.0	41.0	41.0	37.0	41.0
45-49	39.350300000000004	41.0	41.0	41.0	37.0	41.0
50-54	39.16105	41.0	41.0	41.0	37.0	41.0
55-59	39.110549999999996	41.0	41.0	41.0	37.0	41.0
60-64	38.96125000000001	41.0	41.0	41.0	36.0	41.0
65-69	38.76825	41.0	41.0	41.0	33.0	41.0
70-74	38.64445	41.0	41.0	41.0	32.0	41.0
75-79	38.20825000000001	41.0	39.4	41.0	32.0	41.0
80-84	38.46755	41.0	41.0	41.0	32.0	41.0
85-89	38.3794	41.0	41.0	41.0	32.0	41.0
90-94	38.099000000000004	41.0	41.0	41.0	32.0	41.0
95-99	37.850750000000005	41.0	41.0	41.0	28.0	41.0
100-104	37.688199999999995	41.0	38.6	41.0	27.0	41.0
105-109	37.34225	41.0	37.0	41.0	27.0	41.0
110-114	36.9957	41.0	37.0	41.0	22.0	41.0
115-119	36.79455	41.0	37.0	41.0	22.0	41.0
120-124	36.461349999999996	41.0	37.0	41.0	22.0	41.0
125-129	36.1693	41.0	37.0	41.0	22.0	41.0
130-134	35.76520000000001	41.0	37.0	41.0	16.0	41.0
135-139	35.403	41.0	36.0	41.0	12.0	41.0
140-144	35.02995	41.0	32.0	41.0	12.0	41.0
145-149	34.71825	41.0	32.0	41.0	12.0	41.0
150	34.30925	41.0	32.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	5.0
17	9.0
18	14.0
19	9.0
20	9.0
21	14.0
22	11.0
23	10.0
24	26.0
25	19.0
26	34.0
27	32.0
28	47.0
29	41.0
30	58.0
31	58.0
32	63.0
33	87.0
34	111.0
35	136.0
36	121.0
37	198.0
38	328.0
39	683.0
40	1876.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.68126094570928	10.407805854390793	7.705779334500876	40.205153865399055
2	32.25725725725725	17.61761761761762	30.455455455455454	19.66966966966967
3	33.2416208104052	20.785392696348172	18.48424212106053	27.48874437218609
4	31.29064532266133	32.61630815407704	13.681840920460232	22.411205602801402
5	33.4250688016012	33.274956217162874	15.13635226419815	18.16362271703778
6	26.826826826826828	33.28328328328328	15.915915915915916	23.973973973973976
7	29.025000000000002	16.675	29.65	24.65
8	23.78094523630908	19.35483870967742	25.081270317579396	31.782945736434108
9	27.38184546136534	20.130032508127034	25.806451612903224	26.6816704176044
10-14	29.99549842444856	23.38818586505277	21.682588906117143	24.933726804381536
15-19	29.76637150432738	22.06713692530892	22.00710390714893	26.159387663214765
20-24	29.735407392587405	22.32281298454459	22.532886510278598	25.408893112589404
25-29	29.338202190985946	24.89120104046821	19.738882497123704	26.03171427142214
30-34	27.72272272272272	23.65865865865866	20.22022022022022	28.3983983983984
35-39	29.612403100775193	23.63590897724431	19.904976244061015	26.846711677919483
40-44	27.92977542139749	22.96303706297204	21.697594157955287	27.40959335767519
45-49	28.023215089808375	22.829839395607145	21.859208485515584	27.287737029068893
50-54	27.950372704987743	21.89704337385562	22.747511131122117	27.405072790034517
55-59	27.299109376563596	22.680876613629543	22.500750525367756	27.519263484439104
60-64	28.63788704917213	22.55514981741784	22.680206092741734	26.126757040668302
65-69	27.82112845138055	21.46358543417367	21.813725490196077	28.9015606242497
70-74	27.793065492570168	21.85420523340171	21.01866213038475	29.334067143643367
75-79	28.47639055622249	22.073829531812724	22.18887555022009	27.260904361744696
80-84	27.992596668500823	22.825271372117452	22.08493822219999	27.09719373718173
85-89	28.675808227404666	22.06485837253528	22.149934941447302	27.10939845861275
90-94	28.105131414267838	22.618272841051315	21.67709637046308	27.59949937421777
95-99	28.519981993697797	22.847996798879606	20.91732106237183	27.71470014505077
100-104	28.083254115174867	23.27512883374193	21.3939060389253	27.247711012157904
105-109	28.94881673087507	21.649071896732877	21.138740181117726	28.263371191274327
110-114	28.59857957387216	22.7518255476643	21.57147144143243	27.07812343703111
115-119	28.479271890783618	23.188478271740763	21.568235235285293	26.76401460219033
120-124	29.020804160832164	22.999599919983996	21.764352870574115	26.21524304860972
125-129	28.417050230138084	23.70422253352011	21.552931759055433	26.325795477286373
130-134	29.160206072125245	22.71795128294903	22.903016055619467	25.21882658930626
135-139	28.004603222255582	24.982487741418993	20.74452116481537	26.268387871510058
140-144	29.148116652493623	22.750237606923115	23.09539292681707	25.006252813766196
145-149	29.19667867146859	24.499799919967987	20.73829531812725	25.565226090436177
150	26.43821910955478	26.688344172086044	19.43471735867934	27.43871935967984
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.5
13	0.5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5
22	0.5
23	0.0
24	0.5
25	0.5
26	0.5
27	0.5
28	0.0
29	0.5
30	1.0
31	1.0
32	2.5
33	3.0
34	3.0
35	4.0
36	5.0
37	10.5
38	12.5
39	16.0
40	15.0
41	9.0
42	10.5
43	10.0
44	13.5
45	13.5
46	13.5
47	23.5
48	34.5
49	85.5
50	174.0
51	229.5
52	218.0
53	207.0
54	215.5
55	197.5
56	227.5
57	357.0
58	465.0
59	474.0
60	348.0
61	210.0
62	144.5
63	82.0
64	41.5
65	19.5
66	13.5
67	7.0
68	3.5
69	5.5
70	6.0
71	6.0
72	6.0
73	4.5
74	3.5
75	2.5
76	2.0
77	2.5
78	3.0
79	4.5
80	5.0
81	3.0
82	4.0
83	3.5
84	2.5
85	4.5
86	2.5
87	0.0
88	0.0
89	0.5
90	0.5
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.075
2	0.1
3	0.05
4	0.05
5	0.075
6	0.1
7	0.0
8	0.025
9	0.025
10-14	0.034999999999999996
15-19	0.055
20-24	0.034999999999999996
25-29	0.045
30-34	0.1
35-39	0.025
40-44	0.034999999999999996
45-49	0.065
50-54	0.055
55-59	0.06999999999999999
60-64	0.045
65-69	0.04
70-74	0.065
75-79	0.04
80-84	0.045
85-89	0.09
90-94	0.125
95-99	0.034999999999999996
100-104	0.065
105-109	0.065
110-114	0.03
115-119	0.015
120-124	0.02
125-129	0.06
130-134	0.034999999999999996
135-139	0.06999999999999999
140-144	0.045
145-149	0.04
150	0.05
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.55451047456178	45.35
2	9.362975630611373	10.95
3	4.5318512184694315	7.95
4	2.821718683197948	6.6000000000000005
5	1.3253527148353996	3.875
6	1.0260795211628901	3.5999999999999996
7	0.8978195810175289	3.675
8	0.3420265070542967	1.6
9	0.470286447199658	2.475
>10	1.6673792218896963	13.925
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGG	39	0.975	No Hit
GGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACA	25	0.625	No Hit
GTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCC	23	0.575	No Hit
TGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGAC	20	0.5	No Hit
AGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATT	18	0.44999999999999996	No Hit
GGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACGG	17	0.42500000000000004	No Hit
GGAAAAGAGGTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCAGC	17	0.42500000000000004	No Hit
GTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACC	17	0.42500000000000004	No Hit
GTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCT	16	0.4	No Hit
CTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTT	16	0.4	No Hit
CAGGAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAA	16	0.4	No Hit
GTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCAT	15	0.375	No Hit
GGGGAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGA	15	0.375	No Hit
CGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCT	14	0.35000000000000003	No Hit
GGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTT	14	0.35000000000000003	No Hit
GTCAGCACAGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGGCGTGCAT	14	0.35000000000000003	No Hit
CTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAA	14	0.35000000000000003	No Hit
CCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTGCCCAGGTC	13	0.325	No Hit
GGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCAC	13	0.325	No Hit
GTCAACTTCAAGTTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTTGCCA	13	0.325	No Hit
GTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCA	12	0.3	No Hit
CTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTG	12	0.3	No Hit
GACAAAACCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATGGTGG	12	0.3	No Hit
CTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTTCTTGCCG	12	0.3	No Hit
CACAAACTGCGTGTGGATCCTGTCAACTTCAAGTTCCTGAGCCACTGCCT	12	0.3	No Hit
CCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCT	12	0.3	No Hit
GCCCTGTCCACTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCC	12	0.3	No Hit
CGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCTGCAGACCACGTCGAAG	11	0.27499999999999997	No Hit
CTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTGACAAGCTGCA	11	0.27499999999999997	No Hit
GTGCAAGGGAGGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGG	11	0.27499999999999997	No Hit
GGAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCT	11	0.27499999999999997	No Hit
GTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATTGATGTAA	10	0.25	No Hit
CTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGT	10	0.25	No Hit
CCTTGACCTGGGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGTAGGTC	10	0.25	No Hit
GCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCC	10	0.25	No Hit
GTGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATG	10	0.25	No Hit
CTTGGGAACAATTGACCATTGCACAAAGACAAGAGCAGGAAAAGAGGTTT	10	0.25	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	10	0.25	Illumina Single End PCR Primer 1 (100% over 50bp)
GTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTGT	10	0.25	No Hit
GCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACAGGATC	9	0.22499999999999998	No Hit
CTGGGGGAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTAC	9	0.22499999999999998	No Hit
CGAAGACCTGCCTGGTGCCCTGTCCACTCTGAGCGACCTGCATGCCCACA	9	0.22499999999999998	No Hit
AGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATC	9	0.22499999999999998	No Hit
CTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCT	9	0.22499999999999998	No Hit
CTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCCTGTCAACTTC	9	0.22499999999999998	No Hit
GAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCC	9	0.22499999999999998	No Hit
CTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGGGGAAGGCAGCGAA	9	0.22499999999999998	No Hit
CTCGGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCAT	9	0.22499999999999998	No Hit
GTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGA	9	0.22499999999999998	No Hit
CCCGGCTCTGCCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTT	9	0.22499999999999998	No Hit
CACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGCTGAGAAGG	8	0.2	No Hit
CTCACGGCAAGAAGGTTGCTGATGCCCTGGCCAAAGCTGCAGACCACGTC	8	0.2	No Hit
CAGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGT	8	0.2	No Hit
GGGAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGC	8	0.2	No Hit
CCACACGCAGTTTGTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCA	8	0.2	No Hit
AGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGTC	8	0.2	No Hit
GGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACT	8	0.2	No Hit
CCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACC	8	0.2	No Hit
CAGGAGGCGGCTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGC	7	0.17500000000000002	No Hit
GATTTTCAAAGGTCTTTGTCATTTTGACAACAACTGACAGATGCTCTCTT	7	0.17500000000000002	No Hit
CTGCAGATGACAAAACCAACATCAAGAACTGCTGGGGGAAGATTGGTGGC	7	0.17500000000000002	No Hit
CTTTGCTCATCTGAGTGAACTCCACTGTGACAAGCTGCATGTGGATCCTG	7	0.17500000000000002	No Hit
CTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATGG	7	0.17500000000000002	No Hit
GCAGCTTTGGCCAAGGCATCAGCAACCTTCTTGCCGTGAGCCTTGACCTG	7	0.17500000000000002	No Hit
CCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTGTCTACC	7	0.17500000000000002	No Hit
CCACCACCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTGCC	7	0.17500000000000002	No Hit
GTCCACTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCCTGTCA	7	0.17500000000000002	No Hit
GCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGAT	7	0.17500000000000002	No Hit
GCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTG	7	0.17500000000000002	No Hit
CTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTCCTGGG	7	0.17500000000000002	No Hit
GAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCA	7	0.17500000000000002	No Hit
CTGATGCCTTGGCCAAAGCTGCAGACCACGTCGAAGACCTGCCTGGTGCC	7	0.17500000000000002	No Hit
GTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCAGCCACCACCTT	7	0.17500000000000002	No Hit
GTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGA	7	0.17500000000000002	No Hit
CTGGTGCCCTGTCCACTCTGAGCGACCTGCATGCCCACAAACTGCGTGTG	7	0.17500000000000002	No Hit
GGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGG	7	0.17500000000000002	No Hit
GGCGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGAGGTA	7	0.17500000000000002	No Hit
GGGTAACCCTAAGGTGAAGGCCCATGGCAAGAAGGTGATAAACGCCTTCA	7	0.17500000000000002	No Hit
GTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCT	7	0.17500000000000002	No Hit
GGGGACCTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGC	6	0.15	No Hit
GTCGAAGACCTGCCTGGTGCCCTGTCCACTCTGAGCGACCTGCATGCCCA	6	0.15	No Hit
CTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATGGCAAGAAGGTGATA	6	0.15	No Hit
AGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACGGTAC	6	0.15	No Hit
CCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATGGTGGTGAATAT	6	0.15	No Hit
GGGCAATATGATTGTGATTGTGTTGGGCCACCACCTGGGCAAGGAATTCA	6	0.15	No Hit
GGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCA	6	0.15	No Hit
CTTGACCTGGGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGTAGGTCT	6	0.15	No Hit
CAGGCAGTGGCTCAGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGT	6	0.15	No Hit
CTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAATTTG	6	0.15	No Hit
CGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCT	6	0.15	No Hit
CCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTTAATGGCCTGTGG	6	0.15	No Hit
GTGAGCCTTGACCTGGGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGT	6	0.15	No Hit
CTTGGCCAAAGCTGCAGACCACGTCGAAGACCTGCCTGGTGCCCTGTCCA	6	0.15	No Hit
GAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCA	6	0.15	No Hit
GGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGAC	6	0.15	No Hit
CCGGCTCTGCCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTTG	6	0.15	No Hit
GGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACG	6	0.15	No Hit
GTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTAT	6	0.15	No Hit
GCCTGAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAA	6	0.15	No Hit
ATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAG	6	0.15	No Hit
GGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGC	6	0.15	No Hit
CTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCT	6	0.15	No Hit
CTACCCTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTCTG	6	0.15	No Hit
CAGGAAGCAATCATGGTGCTCTCTGCAGCTGACAAAACCAACATCAAGAA	5	0.125	No Hit
CCTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATG	5	0.125	No Hit
GGAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACT	5	0.125	No Hit
GGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGT	5	0.125	No Hit
CTGATGCTGAGAAGGCTGCTGTTAATGGCCTGTGGGGAAAGGTGAACCCT	5	0.125	No Hit
GTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGCT	5	0.125	No Hit
GGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACG	5	0.125	No Hit
TTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAA	5	0.125	No Hit
CTTCTCTCACATTGATGTAAGCCCCGGCTCTGCCCAGGTCAAGGCTCACG	5	0.125	No Hit
CCTGGGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGTAGGTCTTGGTG	5	0.125	No Hit
CCGCCATGCACGCCTCTCTGGACAAATTCCTTGCCTCTGTGAGCACTGTG	5	0.125	No Hit
GGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGG	5	0.125	No Hit
CCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTC	5	0.125	No Hit
CTGAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACT	5	0.125	No Hit
AAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACTGC	5	0.125	No Hit
GGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTGACA	5	0.125	No Hit
CGCAGTTTGTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAG	5	0.125	No Hit
CCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGT	5	0.125	No Hit
GTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTT	5	0.125	No Hit
GCTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAATTT	5	0.125	No Hit
CAGATTTTCAAAGGTCTTTGTCATTTTGACAACAACTGACAGATGCTCTC	5	0.125	No Hit
GTTAATGGCCTGTGGGGAAAGGTGAACCCTGATGATGTTGGTGGCGAGGC	5	0.125	No Hit
CCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCT	5	0.125	No Hit
CCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCTGCTATC	5	0.125	No Hit
CAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAG	5	0.125	No Hit
GTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGT	5	0.125	No Hit
CACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCA	5	0.125	No Hit
AAGAGGTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCAGCCACC	5	0.125	No Hit
AGGGAGGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCT	5	0.125	No Hit
CTTTGGGGACCTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGA	5	0.125	No Hit
CAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.025	0.0	0.0	0.0	0.0
18-19	0.025	0.0	0.0	0.0	0.0
20-21	0.025	0.0	0.0	0.0	0.0
22-23	0.037500000000000006	0.0	0.0	0.0	0.0
24-25	0.05	0.0	0.0	0.0	0.0
26-27	0.05	0.0	0.0	0.0	0.0
28-29	0.05	0.0	0.0	0.0	0.0
30-31	0.05	0.0	0.0	0.0	0.0
32-33	0.05	0.0	0.0	0.0	0.0
34-35	0.125	0.0	0.0	0.0	0.0
36-37	0.175	0.0	0.0	0.0	0.0
38-39	0.1875	0.0	0.0	0.0	0.0
40-41	0.2	0.0	0.0	0.0	0.0
42-43	0.2	0.0	0.0	0.0	0.0
44-45	0.25	0.0	0.0	0.0	0.0
46-47	0.2875	0.0	0.0	0.0	0.0
48-49	0.3125	0.0	0.0	0.0	0.0
50-51	0.325	0.0	0.0	0.0	0.0
52-53	0.4	0.0	0.0	0.0	0.0
54-55	0.475	0.0	0.0	0.0	0.0
56-57	0.475	0.0	0.0	0.0	0.0
58-59	0.475	0.0	0.0	0.0	0.0
60-61	0.475	0.0	0.0	0.0	0.0
62-63	0.5249999999999999	0.0	0.0	0.0	0.0
64-65	0.6375	0.0	0.0	0.0	0.0
66-67	0.675	0.0	0.0	0.0	0.0
68-69	0.7	0.0	0.0	0.0	0.0
70-71	0.75	0.0	0.0	0.0	0.0
72-73	0.825	0.0	0.0	0.0	0.0
74-75	0.875	0.0	0.0	0.0	0.0
76-77	0.95	0.0	0.0	0.0	0.0
78-79	1.0	0.0	0.0	0.0	0.0
80-81	1.0750000000000002	0.0	0.0	0.0	0.0
82-83	1.2625	0.0	0.0	0.0	0.0
84-85	1.45	0.0	0.0	0.0	0.0
86-87	1.575	0.0	0.0	0.0	0.0
88-89	1.625	0.0	0.0	0.0	0.0
90-91	1.8125	0.0	0.0	0.0	0.0
92-93	1.9625	0.0	0.0	0.0	0.0
94-95	2.0875	0.0	0.0	0.0	0.0
96-97	2.375	0.0	0.0	0.0	0.0
98-99	2.6625	0.0	0.0	0.0	0.0
100-101	2.95	0.0	0.0	0.0	0.0
102-103	3.3625	0.0	0.0	0.0	0.0
104-105	3.5875000000000004	0.0	0.0	0.0	0.0
106-107	3.9000000000000004	0.0	0.0	0.0	0.0
108-109	4.237500000000001	0.0	0.0	0.0	0.0
110-111	4.625	0.0	0.0	0.0	0.0
112-113	5.1125	0.0	0.0	0.0	0.0
114-115	5.4875	0.0	0.0	0.0	0.0
116-117	6.012499999999999	0.0	0.0	0.0	0.0
118-119	6.5375	0.0	0.0	0.0	0.0
120-121	6.987500000000001	0.0	0.0	0.0	0.0
122-123	7.475	0.0	0.0	0.0	0.0
124-125	8.2	0.0	0.0	0.0	0.0
126-127	9.0	0.0	0.0	0.0	0.0
128-129	9.6625	0.0	0.0	0.0	0.0
130-131	10.25	0.0	0.0	0.0	0.0
132-133	10.85	0.0	0.0	0.0	0.0
134-135	11.675	0.0	0.0	0.0	0.0
136-137	12.6875	0.0	0.0	0.0	0.0
138	13.275	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTTTTT	20	0.006139246	28.8	15-19
GCCTTGA	40	0.007966741	18.0	70-74
GGCATCA	40	0.007966741	18.0	45-49
CTTGCCG	40	0.007966741	18.0	60-64
CTTTGGC	40	0.007966741	18.0	5
TGGTGGG	55	0.0026350126	15.709091	125-129
>>END_MODULE
Read 3289383 spots for ERR6357090.sra
Written 3289383 spots for ERR6357090.sra
Read 3289387 spots for ERR6357090.sra
Written 3289387 spots for ERR6357090.sra
Read 3289383 spots for ERR6357090.sra
Written 3289383 spots for ERR6357090.sra
Read 3289383 spots for ERR6357090.sra
Written 3289383 spots for ERR6357090.sra
Read 3289383 spots for ERR6357090.sra
Written 3289383 spots for ERR6357090.sra
Read 3289383 spots for ERR6357090.sra
Written 3289383 spots for ERR6357090.sra
Read 3289383 spots for ERR6357090.sra
Written 3289383 spots for ERR6357090.sra
Read 3289383 spots for ERR6357090.sra
Written 3289383 spots for ERR6357090.sra
SRR ids: ['ERR6357090.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_5kxwkjfj
ERR6357090.sra spots: 26315068
blocks: [[1, 3289383], [3289384, 6578766], [6578767, 9868149], [9868150, 13157532], [13157533, 16446915], [16446916, 19736298], [19736299, 23025681], [23025682, 26315068]]
ERR6357090 file size 8844224
ERR6357090 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o ERR6357090 ERR6357090_1.fastq ERR6357090_2.fastq
Input file:	ERR6357090_1.fastq
Paired file:	ERR6357090_2.fastq
trimmed:	ERR6357090-trimmed-pair1.fastq, ERR6357090-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Mon Dec 29 01:46:10 2025 >> started

Mon Dec 29 01:46:58 2025 >> done (47.937s)
26315068 read pairs processed; of these:
    2239 ( 0.01%) short read pairs filtered out after trimming by size control
   80176 ( 0.30%) empty read pairs filtered out after trimming by size control
26232653 (99.69%) read pairs available; of these:
 6101931 (23.26%) trimmed read pairs available after processing
20130722 (76.74%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     296	  0.00%
 19	     341	  0.00%
 20	     331	  0.00%
 21	     486	  0.00%
 22	     530	  0.00%
 23	     546	  0.00%
 24	     579	  0.00%
 25	     634	  0.00%
 26	     669	  0.00%
 27	     893	  0.00%
 28	    1047	  0.00%
 29	    1007	  0.00%
 30	    1239	  0.00%
 31	    1290	  0.00%
 32	    1380	  0.01%
 33	    1666	  0.01%
 34	    1474	  0.01%
 35	    1681	  0.01%
 36	    1930	  0.01%
 37	    1840	  0.01%
 38	    2286	  0.01%
 39	    2119	  0.01%
 40	    2423	  0.01%
 41	    2880	  0.01%
 42	    2751	  0.01%
 43	    3228	  0.01%
 44	    3066	  0.01%
 45	    3189	  0.01%
 46	    3683	  0.01%
 47	    3677	  0.01%
 48	    3769	  0.01%
 49	    4666	  0.02%
 50	    4610	  0.02%
 51	    5204	  0.02%
 52	    5327	  0.02%
 53	    5302	  0.02%
 54	    5494	  0.02%
 55	    5052	  0.02%
 56	    5249	  0.02%
 57	    5929	  0.02%
 58	    5717	  0.02%
 59	    6577	  0.03%
 60	    6130	  0.02%
 61	    7048	  0.03%
 62	    7382	  0.03%
 63	    7135	  0.03%
 64	    7532	  0.03%
 65	    7637	  0.03%
 66	    8827	  0.03%
 67	    8276	  0.03%
 68	   10220	  0.04%
 69	    9188	  0.04%
 70	   10255	  0.04%
 71	   10031	  0.04%
 72	   10722	  0.04%
 73	   11361	  0.04%
 74	   11128	  0.04%
 75	   11093	  0.04%
 76	   10964	  0.04%
 77	   10765	  0.04%
 78	   12844	  0.05%
 79	   12765	  0.05%
 80	   13045	  0.05%
 81	   13843	  0.05%
 82	   16556	  0.06%
 83	   16460	  0.06%
 84	   16745	  0.06%
 85	   16981	  0.06%
 86	   17594	  0.07%
 87	   18802	  0.07%
 88	   19639	  0.07%
 89	   21384	  0.08%
 90	   21306	  0.08%
 91	   22259	  0.08%
 92	   23219	  0.09%
 93	   26673	  0.10%
 94	   29792	  0.11%
 95	   31250	  0.12%
 96	   31630	  0.12%
 97	   27991	  0.11%
 98	   30832	  0.12%
 99	   31324	  0.12%
100	   32758	  0.12%
101	   32245	  0.12%
102	   36070	  0.14%
103	   43678	  0.17%
104	   45309	  0.17%
105	   50946	  0.19%
106	   43003	  0.16%
107	   48269	  0.18%
108	   48067	  0.18%
109	   56346	  0.21%
110	   56351	  0.21%
111	   56116	  0.21%
112	   54358	  0.21%
113	   58743	  0.22%
114	   71144	  0.27%
115	   71684	  0.27%
116	   66833	  0.25%
117	   72940	  0.28%
118	   67861	  0.26%
119	   72301	  0.28%
120	   67514	  0.26%
121	   75737	  0.29%
122	   75049	  0.29%
123	   82755	  0.32%
124	   89298	  0.34%
125	   91344	  0.35%
126	   93853	  0.36%
127	   84644	  0.32%
128	  108047	  0.41%
129	   86040	  0.33%
130	   99603	  0.38%
131	  103722	  0.40%
132	  113257	  0.43%
133	  104761	  0.40%
134	  112043	  0.43%
135	  131476	  0.50%
136	  129515	  0.49%
137	  129432	  0.49%
138	  130651	  0.50%
139	  124947	  0.48%
140	  122745	  0.47%
141	  131221	  0.50%
142	  141408	  0.54%
143	  128178	  0.49%
144	  157701	  0.60%
145	  145404	  0.55%
146	  175620	  0.67%
147	  187658	  0.72%
148	  240070	  0.92%
149	  802531	  3.06%
150	20130722	 76.74%
26232653 reads passed initial QC


criterion=sequence-density
sequence-density=4.85
sequence-density-rank=1
fanout-score=2.39
fanout-score-rank=39
prefix-density=4.78
prefix-fanout=2.4
sequence=GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA


criterion=fanout-score
sequence-density=0.54
sequence-density-rank=20
fanout-score=35.93
fanout-score-rank=1
prefix-density=9.97
prefix-fanout=1.9
sequence=GCAAGGAATTCTCCCCCTGTGCA


criterion=sequence-density
sequence-density=4.67
sequence-density-rank=1
fanout-score=2.41
fanout-score-rank=41
prefix-density=4.60
prefix-fanout=2.4
sequence=GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA


criterion=fanout-score
sequence-density=0.55
sequence-density-rank=19
fanout-score=34.87
fanout-score-rank=1
prefix-density=9.75
prefix-fanout=2.0
sequence=GCAAGGAATTCTCCCCCTGTGCA
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA -y GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA -o ERR6357090 ERR6357090_1.fastq ERR6357090_2.fastq
Input file:	ERR6357090_1.fastq
Paired file:	ERR6357090_2.fastq
trimmed:	ERR6357090-trimmed-pair1.fastq, ERR6357090-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGA
-- paired 3' end adapter sequence (-y):	GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Mon Dec 29 01:48:24 2025 >> started

Mon Dec 29 01:48:59 2025 >> done (35.111s)
15739592 read pairs processed; of these:
    9657 ( 0.06%) short read pairs filtered out after trimming by size control
  125560 ( 0.80%) empty read pairs filtered out after trimming by size control
15604375 (99.14%) read pairs available; of these:
    2723 ( 0.02%) trimmed read pairs available after processing
15601652 (99.98%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     180	  0.00%
 19	     201	  0.00%
 20	     184	  0.00%
 21	     277	  0.00%
 22	     326	  0.00%
 23	     332	  0.00%
 24	     353	  0.00%
 25	     382	  0.00%
 26	     387	  0.00%
 27	     514	  0.00%
 28	     645	  0.00%
 29	     617	  0.00%
 30	     751	  0.00%
 31	     764	  0.00%
 32	     828	  0.01%
 33	     992	  0.01%
 34	     885	  0.01%
 35	    1055	  0.01%
 36	    1169	  0.01%
 37	    1068	  0.01%
 38	    1368	  0.01%
 39	    1292	  0.01%
 40	    1479	  0.01%
 41	    1722	  0.01%
 42	    1632	  0.01%
 43	    1891	  0.01%
 44	    1844	  0.01%
 45	    1925	  0.01%
 46	    2257	  0.01%
 47	    2175	  0.01%
 48	    2282	  0.01%
 49	    2790	  0.02%
 50	    2727	  0.02%
 51	    3129	  0.02%
 52	    3073	  0.02%
 53	    3011	  0.02%
 54	    3036	  0.02%
 55	    2904	  0.02%
 56	    3084	  0.02%
 57	    3504	  0.02%
 58	    3480	  0.02%
 59	    3995	  0.03%
 60	    3632	  0.02%
 61	    4169	  0.03%
 62	    4404	  0.03%
 63	    4230	  0.03%
 64	    4528	  0.03%
 65	    4579	  0.03%
 66	    5266	  0.03%
 67	    4966	  0.03%
 68	    6057	  0.04%
 69	    5504	  0.04%
 70	    6120	  0.04%
 71	    6004	  0.04%
 72	    6376	  0.04%
 73	    6803	  0.04%
 74	    6571	  0.04%
 75	    6614	  0.04%
 76	    6615	  0.04%
 77	    6447	  0.04%
 78	    7618	  0.05%
 79	    7582	  0.05%
 80	    7632	  0.05%
 81	    8180	  0.05%
 82	    9910	  0.06%
 83	    9869	  0.06%
 84	    9993	  0.06%
 85	   10104	  0.06%
 86	   10559	  0.07%
 87	   11247	  0.07%
 88	   11742	  0.08%
 89	   12815	  0.08%
 90	   12829	  0.08%
 91	   13244	  0.08%
 92	   13972	  0.09%
 93	   15935	  0.10%
 94	   17677	  0.11%
 95	   18609	  0.12%
 96	   19030	  0.12%
 97	   16687	  0.11%
 98	   18387	  0.12%
 99	   18569	  0.12%
100	   19478	  0.12%
101	   19310	  0.12%
102	   21633	  0.14%
103	   25993	  0.17%
104	   27175	  0.17%
105	   30452	  0.20%
106	   25724	  0.16%
107	   28760	  0.18%
108	   28934	  0.19%
109	   33604	  0.22%
110	   33500	  0.21%
111	   33478	  0.21%
112	   32529	  0.21%
113	   34990	  0.22%
114	   42559	  0.27%
115	   42881	  0.27%
116	   40104	  0.26%
117	   43658	  0.28%
118	   40428	  0.26%
119	   43078	  0.28%
120	   40348	  0.26%
121	   45254	  0.29%
122	   44866	  0.29%
123	   49633	  0.32%
124	   53159	  0.34%
125	   54499	  0.35%
126	   56221	  0.36%
127	   50443	  0.32%
128	   64913	  0.42%
129	   50769	  0.33%
130	   58618	  0.38%
131	   61409	  0.39%
132	   67482	  0.43%
133	   62723	  0.40%
134	   67134	  0.43%
135	   78675	  0.50%
136	   76645	  0.49%
137	   76506	  0.49%
138	   77350	  0.50%
139	   73370	  0.47%
140	   72487	  0.46%
141	   76958	  0.49%
142	   84319	  0.54%
143	   76127	  0.49%
144	   93797	  0.60%
145	   85823	  0.55%
146	  104000	  0.67%
147	  110176	  0.71%
148	  141680	  0.91%
149	  476536	  3.05%
150	11976602	 76.75%


criterion=sequence-density
sequence-density=4.81
sequence-density-rank=1
fanout-score=2.41
fanout-score-rank=40
prefix-density=4.85
prefix-fanout=2.4
sequence=GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA


criterion=fanout-score
sequence-density=0.54
sequence-density-rank=20
fanout-score=36.03
fanout-score-rank=1
prefix-density=9.97
prefix-fanout=1.9
sequence=GCAAGGAATTCTCCCCCTGTGCA


criterion=sequence-density
sequence-density=4.60
sequence-density-rank=1
fanout-score=2.42
fanout-score-rank=38
prefix-density=4.64
prefix-fanout=2.4
sequence=GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA


criterion=fanout-score
sequence-density=0.53
sequence-density-rank=19
fanout-score=36.17
fanout-score-rank=1
prefix-density=9.75
prefix-fanout=2.0
sequence=GCAAGGAATTCTCCCCCTGTGCA
ERR6357090 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Dec 29 01:49:49
                             Started mapping on |	Dec 29 01:49:49
                                    Finished on |	Dec 29 02:00:27
       Mapping speed, Million of reads per hour |	147.26

                          Number of input reads |	26097436
                      Average input read length |	289
                                    UNIQUE READS:
                   Uniquely mapped reads number |	19983545
                        Uniquely mapped reads % |	76.57%
                          Average mapped length |	288.01
                       Number of splices: Total |	25523773
            Number of splices: Annotated (sjdb) |	25007196
                       Number of splices: GT/AG |	25405451
                       Number of splices: GC/AG |	24035
                       Number of splices: AT/AC |	1780
               Number of splices: Non-canonical |	92507
                      Mismatch rate per base, % |	0.47%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.97
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.74
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	5357361
             % of reads mapped to multiple loci |	20.53%
        Number of reads mapped to too many loci |	65208
             % of reads mapped to too many loci |	0.25%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	2.63%
                     % of reads unmapped: other |	0.02%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	756530	756530	756530
N_multimapping	5357361	5357361	5357361
N_noFeature	322454	10066549	10152065
N_ambiguous	376033	140049	148916
UnstrandedReadsAssigned:19285058 PositiveStrandReadsAssigned:9776947 NegativeStrandReadsAssigned:9682564
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=148 echo kmer=143
ERR6357090 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 31,715
[index] number of k-mers: 49,915,185
[index] number of equivalence classes: 70,351
[quant] running in paired-end mode
[quant] will process pair 1: ERR6357090-trimmed-pair1.fastq
                             ERR6357090-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 26,097,436 reads, 21,401,217 reads pseudoaligned
[quant] estimated average fragment length: 192.092
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 989 rounds

  31716 ERR6357090.ke.tsv
  32883 ERR6357090.se.tsv
  64599 total
==> ERR6357090.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	763.908	2095	54.7184
ENSRNOT00000040993.4	1039	847.908	1491	35.0848
ENSRNOT00000050156.3	1545	1353.91	16914	249.257
ENSRNOT00000043693.3	684	493.013	4586	185.595
ENSRNOT00000046201.3	204	49.7928	156	62.5097
ENSRNOT00000046108.3	681	490.013	5285	215.193
ENSRNOT00000049683.3	784	592.941	8485	285.516
ENSRNOT00000041241.3	348	159.253	277	34.7042
ENSRNOT00000044582.3	297	113.381	95	16.7176

==> ERR6357090.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	0
ENSRNOG00000058808	0
ENSRNOG00000061316	0
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	0
ERR6357090 completed mapping pipeline successfully
