Starting /dee2/code/volunteer_pipeline.sh ERR6357086
    current disk space = 205210912
    free memory = 193446984 
ERR6357086 SRAfilesize
c66c360ea10e8a8ee1dff63069ff59c9  ERR6357086.sra
ERR6357086.sra file validated
ERR6357086 is paired end
ERR6357086 is conventional basespace
ERR6357086 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR6357086_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53875	32.0	32.0	32.0	32.0	32.0
2	31.405	32.0	32.0	32.0	32.0	32.0
3	35.3825	37.0	37.0	37.0	32.0	37.0
4	36.03625	37.0	37.0	37.0	32.0	37.0
5	36.355	37.0	37.0	37.0	37.0	37.0
6	39.96275	41.0	41.0	41.0	37.0	41.0
7	40.042	41.0	41.0	41.0	37.0	41.0
8	40.1175	41.0	41.0	41.0	37.0	41.0
9	40.06475	41.0	41.0	41.0	37.0	41.0
10-14	40.07955	41.0	41.0	41.0	37.0	41.0
15-19	39.94644999999999	41.0	41.0	41.0	37.0	41.0
20-24	39.786350000000006	41.0	41.0	41.0	37.0	41.0
25-29	39.46295	41.0	41.0	41.0	37.0	41.0
30-34	39.2135	41.0	41.0	41.0	37.0	41.0
35-39	39.0018	41.0	41.0	41.0	37.0	41.0
40-44	38.78595	41.0	41.0	41.0	34.0	41.0
45-49	38.54015	41.0	40.2	41.0	32.0	41.0
50-54	38.2937	41.0	37.8	41.0	32.0	41.0
55-59	37.98565	41.0	37.0	41.0	30.0	41.0
60-64	37.6531	41.0	37.0	41.0	27.0	41.0
65-69	37.43589999999999	41.0	37.0	41.0	27.0	41.0
70-74	36.872049999999994	41.0	37.0	41.0	27.0	41.0
75-79	36.8167	41.0	37.0	41.0	27.0	41.0
80-84	37.13875	41.0	37.0	41.0	26.0	41.0
85-89	36.89919999999999	41.0	37.0	41.0	25.0	41.0
90-94	36.565450000000006	41.0	37.0	41.0	22.0	41.0
95-99	36.175850000000004	41.0	37.0	41.0	22.0	41.0
100-104	35.8636	41.0	34.0	41.0	22.0	41.0
105-109	35.44735	41.0	32.0	41.0	22.0	41.0
110-114	35.155499999999996	41.0	32.0	41.0	22.0	41.0
115-119	34.72425	41.0	32.0	41.0	12.0	41.0
120-124	34.35945	41.0	32.0	41.0	12.0	41.0
125-129	33.803749999999994	37.8	29.0	41.0	12.0	41.0
130-134	33.36045	37.0	27.0	41.0	12.0	41.0
135-139	32.7599	37.0	26.0	41.0	12.0	41.0
140-144	32.312799999999996	37.0	24.0	41.0	12.0	41.0
145-149	31.75415	37.0	22.0	41.0	12.0	41.0
150	31.5015	37.0	22.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	4.0
19	4.0
20	5.0
21	13.0
22	14.0
23	22.0
24	29.0
25	44.0
26	66.0
27	46.0
28	43.0
29	68.0
30	69.0
31	93.0
32	111.0
33	165.0
34	176.0
35	256.0
36	324.0
37	488.0
38	561.0
39	585.0
40	809.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.870935467733865	11.730865432716358	7.878939469734868	38.519259629814904
2	31.85	16.975	32.425	18.75
3	32.85	21.875	20.05	25.224999999999998
4	29.875	32.4	15.25	22.475
5	31.7	33.6	16.275000000000002	18.425
6	26.825	33.85	14.975	24.349999999999998
7	27.05	17.5	30.599999999999998	24.85
8	23.275000000000002	20.625	26.0	30.099999999999998
9	28.175	19.05	25.825	26.950000000000003
10-14	29.015	24.18	21.42	25.385
15-19	29.830000000000002	22.720000000000002	21.66	25.790000000000003
20-24	28.93	22.925	22.475	25.669999999999998
25-29	28.810000000000002	25.095	19.505	26.590000000000003
30-34	28.63	24.625	19.55	27.195000000000004
35-39	28.985	24.435000000000002	20.115	26.465
40-44	27.665	22.86	21.91	27.565
45-49	27.955000000000002	22.59	22.445	27.01
50-54	27.834999999999997	21.790000000000003	22.905	27.47
55-59	28.13	23.015	22.45	26.405
60-64	28.375	22.325	22.925	26.375
65-69	27.474999999999998	21.525	22.5	28.499999999999996
70-74	27.834999999999997	21.93	22.13	28.105000000000004
75-79	29.294999999999998	21.87	22.05	26.784999999999997
80-84	27.91	24.015	22.085	25.990000000000002
85-89	28.365000000000002	22.439999999999998	22.39	26.805
90-94	28.205000000000002	21.865000000000002	22.2	27.73
95-99	28.199999999999996	22.185	21.51	28.105000000000004
100-104	28.585	22.895	21.834999999999997	26.685
105-109	28.17	21.43	22.25	28.15
110-114	28.51	21.945	21.665	27.88
115-119	27.61	23.46	21.759999999999998	27.169999999999998
120-124	27.865000000000002	22.875	21.905	27.355
125-129	26.700000000000003	23.79	22.0	27.51
130-134	27.91	22.405	23.32	26.365
135-139	26.465	24.38	21.175	27.98
140-144	27.3	23.28	23.005	26.415
145-149	26.615	24.445	21.075	27.865000000000002
150	24.55	26.125	20.8	28.525
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.5
30	1.5
31	2.0
32	1.0
33	2.5
34	4.0
35	2.5
36	5.0
37	10.0
38	12.5
39	17.0
40	13.5
41	10.0
42	12.5
43	13.0
44	15.0
45	15.0
46	18.5
47	22.5
48	37.0
49	99.5
50	210.0
51	270.5
52	258.5
53	224.0
54	196.5
55	196.0
56	237.0
57	384.5
58	481.5
59	422.0
60	301.5
61	189.5
62	113.5
63	63.5
64	38.0
65	17.5
66	9.5
67	7.5
68	4.5
69	3.5
70	4.0
71	3.5
72	4.5
73	3.5
74	1.5
75	1.5
76	2.5
77	4.0
78	4.5
79	4.0
80	3.5
81	3.0
82	3.5
83	4.0
84	2.5
85	1.5
86	2.0
87	1.5
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.7
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.62756052141528	40.075
2	9.543761638733706	10.25
3	5.21415270018622	8.4
4	2.653631284916201	5.7
5	2.3743016759776534	6.375
6	1.1638733705772812	3.75
7	1.0707635009310987	4.025
8	0.8379888268156425	3.5999999999999996
9	0.5121042830540037	2.475
>10	2.001862197392924	15.35
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGG	30	0.75	No Hit
GGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCAC	27	0.675	No Hit
GGAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCT	25	0.625	No Hit
GTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCT	22	0.5499999999999999	No Hit
GTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCC	22	0.5499999999999999	No Hit
GTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACC	22	0.5499999999999999	No Hit
CTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCT	19	0.475	No Hit
CAGGAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAA	18	0.44999999999999996	No Hit
GTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCA	16	0.4	No Hit
GCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTC	16	0.4	No Hit
GGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACGG	15	0.375	No Hit
TGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGAC	15	0.375	No Hit
GCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCC	15	0.375	No Hit
CTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTT	15	0.375	No Hit
CTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTGACAAGCTGCA	15	0.375	No Hit
GGAAAAGAGGTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCAGC	15	0.375	No Hit
GAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCC	14	0.35000000000000003	No Hit
CTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTG	13	0.325	No Hit
GTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGCT	13	0.325	No Hit
CGGGTGTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAG	13	0.325	No Hit
GTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTGT	13	0.325	No Hit
CCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTGCCCAGGTC	12	0.3	No Hit
GACAAAACCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATGGTGG	12	0.3	No Hit
AGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACGGTAC	12	0.3	No Hit
GGGAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGC	12	0.3	No Hit
GTTGTGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTG	12	0.3	No Hit
GGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTT	12	0.3	No Hit
GGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACA	12	0.3	No Hit
CTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAA	12	0.3	No Hit
GTGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATG	11	0.27499999999999997	No Hit
GGGGAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGA	11	0.27499999999999997	No Hit
GCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTG	11	0.27499999999999997	No Hit
GGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGG	11	0.27499999999999997	No Hit
CCCGGCTCTGCCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTT	11	0.27499999999999997	No Hit
CTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGG	10	0.25	No Hit
AAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACTGC	10	0.25	No Hit
CTTGGGAACAATTGACCATTGCACAAAGACAAGAGCAGGAAAAGAGGTTT	10	0.25	No Hit
GTGCAAGGGAGGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGG	10	0.25	No Hit
GTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTAT	10	0.25	No Hit
GGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACT	10	0.25	No Hit
CCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGC	10	0.25	No Hit
CTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCA	10	0.25	No Hit
TGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGC	10	0.25	No Hit
CGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCTGCAGACCACGTCGAAG	9	0.22499999999999998	No Hit
GGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACG	9	0.22499999999999998	No Hit
CACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGCTGAGAAGG	9	0.22499999999999998	No Hit
GAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTG	9	0.22499999999999998	No Hit
CTGAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACT	9	0.22499999999999998	No Hit
CTTGACCTGGGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGTAGGTCT	9	0.22499999999999998	No Hit
GTCCACTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCCTGTCA	9	0.22499999999999998	No Hit
GAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCA	9	0.22499999999999998	No Hit
GTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCT	9	0.22499999999999998	No Hit
CTCACGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCTGCAGACCACGTC	9	0.22499999999999998	No Hit
GTCAACTTCAAGTTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTTGCCA	9	0.22499999999999998	No Hit
GGCGGCTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGA	8	0.2	No Hit
GGAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACT	8	0.2	No Hit
GGTAACCCTAAGGTGAAGGCCCATGGCAAGAAGGTGATAAACGCCTTCAA	8	0.2	No Hit
CTTTGCTCATCTGAGTGAACTCCACTGTGACAAGCTGCATGTGGATCCTG	8	0.2	No Hit
CAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACAT	8	0.2	No Hit
GGGCAATATGATTGTGATTGTGTTGGGCCACCACCTGGGCAAGGAATTCA	8	0.2	No Hit
CCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTC	8	0.2	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC	8	0.2	TruSeq Adapter, Index 9 (100% over 50bp)
AGATGACAAAACCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATG	8	0.2	No Hit
ACAGGATCCACACGCAGTTTGTGGGCATGCAGGTCGCTCAGAGTGGACAG	8	0.2	No Hit
CCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCT	8	0.2	No Hit
CCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTT	8	0.2	No Hit
CTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCCTGTCAACTTC	8	0.2	No Hit
CTCGGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCAT	8	0.2	No Hit
GTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGA	8	0.2	No Hit
GGGACCTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCC	8	0.2	No Hit
CTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCT	8	0.2	No Hit
GGGAGGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTT	8	0.2	No Hit
CTTGGGAACAATTGACCAATGCACAAAGACAAGAGCAGGAAAAGAGGTTT	7	0.17500000000000002	No Hit
GCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACAGGATC	7	0.17500000000000002	No Hit
GTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATTGATGTAA	7	0.17500000000000002	No Hit
CTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGT	7	0.17500000000000002	No Hit
GTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCAT	7	0.17500000000000002	No Hit
CCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATGGTGGTGAATAT	7	0.17500000000000002	No Hit
GTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGG	7	0.17500000000000002	No Hit
CAGGCAGTGGCTCAGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGT	7	0.17500000000000002	No Hit
CTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTTCTTGCCG	7	0.17500000000000002	No Hit
GGAATTTGTCCAGAGAGGCGTGCATGGCGGGTGTGAAATCTCCAGGGTGG	7	0.17500000000000002	No Hit
AACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCAC	7	0.17500000000000002	No Hit
AGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATT	7	0.17500000000000002	No Hit
CGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCT	7	0.17500000000000002	No Hit
CAAGAAGGTGATAAACGCCTTCAATGATGGCCTGAAACACTTGGACAACC	7	0.17500000000000002	No Hit
CAGATTTTCAAAGGTCTTTGTCATTTTGACAACAACTGACAGATGCTCTC	7	0.17500000000000002	No Hit
GGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGAC	7	0.17500000000000002	No Hit
CTGATGCCTTGGCCAAAGCTGCAGACCACGTCGAAGACCTGCCTGGTGCC	7	0.17500000000000002	No Hit
GTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCAGCCACCACCTT	7	0.17500000000000002	No Hit
GTCAGCACAGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGGCGTGCAT	7	0.17500000000000002	No Hit
CCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCT	7	0.17500000000000002	No Hit
AGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGTC	7	0.17500000000000002	No Hit
GTCTACCCTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTC	7	0.17500000000000002	No Hit
GTGAAGGCCCATGGCAAGAAGGTGATAAACGCCTTCAATGATGGCCTGAA	7	0.17500000000000002	No Hit
AAGAAGGTGATAAACGCCTTCAATGATGGCCTGAAACACTTGGACAACCT	6	0.15	No Hit
GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATT	6	0.15	No Hit
GATTTTCAAAGGTCTTTGTCATTTTGACAACAACTGACAGATGCTCTCTT	6	0.15	No Hit
GGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTG	6	0.15	No Hit
GGCGGGTGTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGC	6	0.15	No Hit
GAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATTG	6	0.15	No Hit
CTAAGGTGAAGGCCCATGGCAAGAAGGTGATAAACGCCTTCAATGATGGC	6	0.15	No Hit
GAACAATTGACCATTGCACAAAGACAAGAGCAGGAAAAGAGGTTTAGTGG	6	0.15	No Hit
CACAAACTGCGTGTGGATCCTGTCAACTTCAAGTTCCTGAGCCACTGCCT	6	0.15	No Hit
CCTGCATGCCCACAAACTGCGTGTGGATCCTGTCAACTTCAAGTTCCTGA	6	0.15	No Hit
GTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCTGCAGA	6	0.15	No Hit
ATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGC	6	0.15	No Hit
GTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACAGGATCCACACG	6	0.15	No Hit
CGGCTCTGCCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTTGG	6	0.15	No Hit
CCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTTAATGGCCTGTGG	6	0.15	No Hit
GTGAAAGTAAATGCCTTTTATTAGTAGGACAGATTTTCAAAGGTCTTTGT	6	0.15	No Hit
GTGAAATCCCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCT	6	0.15	No Hit
TGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTT	6	0.15	No Hit
CCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCTGCTATC	6	0.15	No Hit
GTTGACTCGCAACCTCAGGAACAGACACCATGGTGCACCTAACTGATGCT	6	0.15	No Hit
GGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACG	6	0.15	No Hit
GGGTAACCCTAAGGTGAAGGCCCATGGCAAGAAGGTGATAAACGCCTTCA	6	0.15	No Hit
GCCCTGTCCACTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCC	6	0.15	No Hit
CCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACC	6	0.15	No Hit
CCTGTGCACTCGGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATC	6	0.15	No Hit
GGGGACCTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGC	5	0.125	No Hit
CGGCAAGAAGGTTGCTGATGCCCTGGCCAAAGCTGCAGACCACGTCGAAG	5	0.125	No Hit
CCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGAGGTACTTTGATA	5	0.125	No Hit
CACAAAGACAAGAGCAGGAAAAGAGGTTTAGTGGTACTTGTGAGCCAGGG	5	0.125	No Hit
CCACAAACTGCGTGTGGATCCTGTCAACTTCAAGTTCCTGAGCCACTGCC	5	0.125	No Hit
CTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATGG	5	0.125	No Hit
AAACGCCTTCAATGATGGCCTGAAACACTTGGACAACCTCAAGGGCACCT	5	0.125	No Hit
GTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTTCTTGC	5	0.125	No Hit
GGCGTGCATGGCGGGTGTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCA	5	0.125	No Hit
GGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAATTTGTCCAGA	5	0.125	No Hit
CAAGAAGGTTGCTGATGCCTTGGCCAAAGCTGCAGACCACGTCGAAGACC	5	0.125	No Hit
CAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGT	5	0.125	No Hit
AGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATC	5	0.125	No Hit
CCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCA	5	0.125	No Hit
GCTGGTTGTCTACCCTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACC	5	0.125	No Hit
GGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGG	5	0.125	No Hit
AAAAGAGGTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCAGCCA	5	0.125	No Hit
GTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATGGCA	5	0.125	No Hit
CCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTGTCTACC	5	0.125	No Hit
GAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACGGT	5	0.125	No Hit
CAGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGT	5	0.125	No Hit
CCACCACCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTGCC	5	0.125	No Hit
ATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGT	5	0.125	No Hit
CGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCT	5	0.125	No Hit
GCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGAT	5	0.125	No Hit
TGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTC	5	0.125	No Hit
GGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGC	5	0.125	No Hit
CGCAGTTTGTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAG	5	0.125	No Hit
AGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGA	5	0.125	No Hit
AAGGGAGGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGC	5	0.125	No Hit
CTTGGCCAAAGCTGCAGACCACGTCGAAGACCTGCCTGGTGCCCTGTCCA	5	0.125	No Hit
GGGCACCTTTGCTCATCTGAGTGAACTCCACTGTGACAAGCTGCATGTGG	5	0.125	No Hit
CTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGCTGAGAA	5	0.125	No Hit
CAAAACCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATGGTGGTG	5	0.125	No Hit
CCCACCACCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTGC	5	0.125	No Hit
CAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGCTGAGAAGGCT	5	0.125	No Hit
GAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCA	5	0.125	No Hit
GGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATTGAT	5	0.125	No Hit
CTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAA	5	0.125	No Hit
CACCACCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTGCCC	5	0.125	No Hit
CCCATGGCAAGAAGGTGATAAACGCCTTCAATGATGGCCTGAAACACTTG	5	0.125	No Hit
CCCAGGTGGTGGCCCAACACAATCACAATCATGTTGCCCAGGAGCCTGAA	5	0.125	No Hit
CTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAA	5	0.125	No Hit
CAGACACCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTTAATGGC	5	0.125	No Hit
CTTAACGGTACTTGGAGGTCAGCACGGTGCTCACAGAGGCAAGGAATTTG	5	0.125	No Hit
GTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACGGTACTTGGAGGTCAG	5	0.125	No Hit
GTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGA	5	0.125	No Hit
GAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACTG	5	0.125	No Hit
CCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGAGGTACTTTGAT	5	0.125	No Hit
ATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAG	5	0.125	No Hit
CTGCTGGTGACCTTGGCTTGCCACCACCCTGGAGATTTCACACCCGCCAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0125	0.0	0.0	0.0	0.0
26-27	0.025	0.0	0.0	0.0	0.0
28-29	0.025	0.0	0.0	0.0	0.0
30-31	0.05	0.0	0.0	0.0	0.0
32-33	0.075	0.0	0.0	0.0	0.0
34-35	0.075	0.0	0.0	0.0	0.0
36-37	0.075	0.0	0.0	0.0	0.0
38-39	0.075	0.0	0.0	0.0	0.0
40-41	0.075	0.0	0.0	0.0	0.0
42-43	0.125	0.0	0.0	0.0	0.0
44-45	0.15	0.0	0.0	0.0	0.0
46-47	0.2	0.0	0.0	0.0	0.0
48-49	0.2	0.0	0.0	0.0	0.0
50-51	0.225	0.0	0.0	0.0	0.0
52-53	0.3125	0.0	0.0	0.0	0.0
54-55	0.3375	0.0	0.0	0.0	0.0
56-57	0.35	0.0	0.0	0.0	0.0
58-59	0.35	0.0	0.0	0.0	0.0
60-61	0.375	0.0	0.0	0.0	0.0
62-63	0.4125	0.0	0.0	0.0	0.0
64-65	0.425	0.0	0.0	0.0	0.0
66-67	0.4625	0.0	0.0	0.0	0.0
68-69	0.5125	0.0	0.0	0.0	0.0
70-71	0.65	0.0	0.0	0.0	0.0
72-73	0.6875	0.0	0.0	0.0	0.0
74-75	0.775	0.0	0.0	0.0	0.0
76-77	0.875	0.0	0.0	0.0	0.0
78-79	0.95	0.0	0.0	0.0	0.0
80-81	1.1124999999999998	0.0	0.0	0.0	0.0
82-83	1.1875	0.0	0.0	0.0	0.0
84-85	1.325	0.0	0.0	0.0	0.0
86-87	1.4375	0.0	0.0	0.0	0.0
88-89	1.575	0.0	0.0	0.0	0.0
90-91	1.625	0.0	0.0	0.0	0.0
92-93	1.725	0.0	0.0	0.0	0.0
94-95	1.8625	0.0	0.0	0.0	0.0
96-97	2.0625	0.0	0.0	0.0	0.0
98-99	2.2249999999999996	0.0	0.0	0.0	0.0
100-101	2.3499999999999996	0.0	0.0	0.0	0.0
102-103	2.5999999999999996	0.0	0.0	0.0	0.0
104-105	2.9375	0.0	0.0	0.0	0.0
106-107	3.2375	0.0	0.0	0.0	0.0
108-109	3.4749999999999996	0.0	0.0	0.0	0.0
110-111	3.7874999999999996	0.0	0.0	0.0	0.0
112-113	4.137499999999999	0.0	0.0	0.0	0.0
114-115	4.4375	0.0	0.0	0.0	0.0
116-117	4.8125	0.0	0.0	0.0	0.0
118-119	5.2125	0.0	0.0	0.0	0.0
120-121	5.6	0.0	0.0	0.0	0.0
122-123	5.9125	0.0	0.0	0.0	0.0
124-125	6.4625	0.0	0.0	0.0	0.0
126-127	7.012499999999999	0.0	0.0	0.0	0.0
128-129	7.574999999999999	0.0	0.0	0.0	0.0
130-131	8.1125	0.0	0.0	0.0	0.0
132-133	8.65	0.0	0.0	0.0	0.0
134-135	9.2875	0.0	0.0	0.0	0.0
136-137	10.125	0.0	0.0	0.0	0.0
138	10.65	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
ERR6357086 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR6357086_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72125	32.0	32.0	32.0	32.0	32.0
2	31.54375	32.0	32.0	32.0	32.0	32.0
3	35.57125	37.0	37.0	37.0	32.0	37.0
4	36.135	37.0	37.0	37.0	37.0	37.0
5	36.33625	37.0	37.0	37.0	37.0	37.0
6	39.933	41.0	41.0	41.0	37.0	41.0
7	39.9825	41.0	41.0	41.0	37.0	41.0
8	39.952	41.0	41.0	41.0	37.0	41.0
9	40.0785	41.0	41.0	41.0	41.0	41.0
10-14	40.02204999999999	41.0	41.0	41.0	39.4	41.0
15-19	39.9329	41.0	41.0	41.0	37.0	41.0
20-24	39.82965	41.0	41.0	41.0	37.0	41.0
25-29	39.80985	41.0	41.0	41.0	37.0	41.0
30-34	39.6858	41.0	41.0	41.0	37.0	41.0
35-39	39.5509	41.0	41.0	41.0	37.0	41.0
40-44	39.467650000000006	41.0	41.0	41.0	37.0	41.0
45-49	39.240249999999996	41.0	41.0	41.0	37.0	41.0
50-54	39.1384	41.0	41.0	41.0	37.0	41.0
55-59	39.07955	41.0	41.0	41.0	37.0	41.0
60-64	38.86095	41.0	41.0	41.0	34.0	41.0
65-69	38.68845	41.0	41.0	41.0	32.0	41.0
70-74	38.4435	41.0	41.0	41.0	32.0	41.0
75-79	37.95285	41.0	39.4	41.0	31.0	41.0
80-84	38.31505	41.0	41.0	41.0	32.0	41.0
85-89	38.122699999999995	41.0	41.0	41.0	32.0	41.0
90-94	37.93384999999999	41.0	40.2	41.0	29.0	41.0
95-99	37.75165	41.0	40.2	41.0	27.0	41.0
100-104	37.4288	41.0	37.0	41.0	27.0	41.0
105-109	37.151300000000006	41.0	37.0	41.0	23.0	41.0
110-114	36.89885	41.0	37.0	41.0	22.0	41.0
115-119	36.60355	41.0	37.0	41.0	22.0	41.0
120-124	36.26	41.0	37.0	41.0	22.0	41.0
125-129	35.96810000000001	41.0	37.0	41.0	20.0	41.0
130-134	35.684749999999994	41.0	37.0	41.0	16.0	41.0
135-139	35.3389	41.0	36.0	41.0	12.0	41.0
140-144	34.8292	41.0	32.0	41.0	12.0	41.0
145-149	34.4724	41.0	32.0	41.0	12.0	41.0
150	34.28775	41.0	32.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	4.0
16	6.0
17	5.0
18	13.0
19	9.0
20	12.0
21	11.0
22	18.0
23	14.0
24	20.0
25	27.0
26	45.0
27	44.0
28	34.0
29	38.0
30	64.0
31	68.0
32	81.0
33	93.0
34	89.0
35	104.0
36	147.0
37	212.0
38	304.0
39	677.0
40	1859.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.56039009752438	10.602650662665667	8.127031757939484	39.709927481870466
2	31.732933233308323	16.55413853463366	32.98324581145287	18.72968242060515
3	34.14207103551776	20.910455227613806	19.009504752376188	25.937968984492244
4	32.10802700675169	32.0830207551888	14.378594648662165	21.43035758939735
5	32.93323330832708	33.408352088022006	16.60415103775944	17.05426356589147
6	26.231557889472366	32.6081520380095	17.129282320580145	24.031007751937985
7	28.93223305826457	17.30432608152038	29.732433108277068	24.031007751937985
8	24.637318659329665	20.110055027513756	25.18759379689845	30.06503251625813
9	26.713356678339167	20.885442721360683	24.437218609304654	27.963981990995496
10-14	29.310258590506677	24.18846596308708	21.47751713099585	25.023758315410394
15-19	29.86746686671668	22.72568142035509	21.660415103775943	25.74643660915229
20-24	29.096638655462186	22.624049619847938	22.524009603841534	25.755302120848338
25-29	29.473842152645798	25.17255176552966	19.700910273081927	25.65269580874262
30-34	28.328498549564866	23.937181154346305	20.681204361308392	27.053115934780436
35-39	28.776510604241697	24.12464985994398	20.36314525810324	26.735694277711087
40-44	28.37209302325581	22.1505376344086	21.955488872218055	27.521880470117527
45-49	27.996198479391754	22.7641056422569	22.093837535014003	27.145858343337338
50-54	27.257265769596316	22.580161072482618	22.90030513731179	27.262268020609277
55-59	28.249887460611212	22.99304756664833	21.95268343920372	26.804381533536738
60-64	28.266306522609042	22.904161664665867	22.669067627050822	26.16046418567427
65-69	27.327297283777703	21.55469961482667	22.360062027912562	28.757941073483067
70-74	28.294903216125643	21.587555644475568	21.877657180013006	28.239883959385786
75-79	29.034517258629318	22.181090545272635	22.12606303151576	26.65832916458229
80-84	27.36957935277347	23.888360926324214	22.14274996248687	26.599309758415444
85-89	28.122655194837677	22.44510029513281	22.880296133259968	26.551948376769545
90-94	28.267720474213398	22.730228602871293	21.609724375969186	27.392326546946126
95-99	28.7943971985993	21.965982991495746	21.30565282641321	27.933966983491747
100-104	27.92977542139749	23.70829790426649	21.757615165307858	26.60431150902816
105-109	28.840094032911516	21.61256439753914	22.002700945330865	27.544640624218474
110-114	28.394197098549274	22.18609304652326	21.70585292646323	27.71385692846423
115-119	28.771508603441376	22.799119647859143	22.008803521408563	26.420568227290914
120-124	28.421368547418968	22.95918367346939	22.103841536614645	26.515606242496997
125-129	27.582412085438445	23.47556400380171	22.03991796308339	26.902105947676453
130-134	29.06307838527337	23.120404181881845	23.05537491871342	24.76114251413136
135-139	27.772497623930768	24.430993947276274	21.524686108748938	26.27182232004402
140-144	29.231692677070832	22.739095638255304	22.99419767907163	25.035014005602243
145-149	28.179089544772385	24.85742871435718	21.11055527763882	25.85292646323162
150	26.96348174087044	25.46273136568284	20.885442721360683	26.688344172086044
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.5
27	0.5
28	1.0
29	1.5
30	1.0
31	1.0
32	2.0
33	2.5
34	2.5
35	3.5
36	5.5
37	9.0
38	11.0
39	15.5
40	18.0
41	13.5
42	13.5
43	13.5
44	15.5
45	18.0
46	14.5
47	20.0
48	42.0
49	99.0
50	194.0
51	265.5
52	261.0
53	230.0
54	210.0
55	198.0
56	238.5
57	359.0
58	443.5
59	421.5
60	315.5
61	194.5
62	123.0
63	74.5
64	38.0
65	15.5
66	14.5
67	13.5
68	6.0
69	3.5
70	4.5
71	4.0
72	4.0
73	2.5
74	1.5
75	4.0
76	4.0
77	3.0
78	4.0
79	4.0
80	2.5
81	3.0
82	3.0
83	3.0
84	3.0
85	2.5
86	1.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.025
3	0.05
4	0.025
5	0.025
6	0.025
7	0.025
8	0.05
9	0.05
10-14	0.034999999999999996
15-19	0.025
20-24	0.04
25-29	0.03
30-34	0.03
35-39	0.04
40-44	0.025
45-49	0.04
50-54	0.045
55-59	0.034999999999999996
60-64	0.04
65-69	0.045
70-74	0.034999999999999996
75-79	0.05
80-84	0.034999999999999996
85-89	0.045
90-94	0.045
95-99	0.05
100-104	0.034999999999999996
105-109	0.034999999999999996
110-114	0.05
115-119	0.04
120-124	0.04
125-129	0.045
130-134	0.045
135-139	0.045
140-144	0.04
145-149	0.05
150	0.05
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.85
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.18236819360415	44.65
2	9.075194468452894	10.5
3	4.969749351771824	8.625
4	2.8522039757994815	6.6000000000000005
5	1.5557476231633534	4.5
6	0.9075194468452896	3.15
7	1.1235955056179776	4.55
8	0.5617977528089888	2.6
9	0.34572169403630076	1.7999999999999998
>10	1.4261019878997407	13.025
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCC	32	0.8	No Hit
GGAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGG	29	0.7250000000000001	No Hit
GTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCT	27	0.675	No Hit
GTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACC	27	0.675	No Hit
CTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTT	22	0.5499999999999999	No Hit
GGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACA	22	0.5499999999999999	No Hit
CAGGAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAA	21	0.525	No Hit
GGAAAAGAGGTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCAGC	20	0.5	No Hit
GTCAACTTCAAGTTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTTGCCA	20	0.5	No Hit
GGAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCT	20	0.5	No Hit
GGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGG	19	0.475	No Hit
GTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGCT	18	0.44999999999999996	No Hit
CTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTGACAAGCTGCA	15	0.375	No Hit
GGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACGG	14	0.35000000000000003	No Hit
GTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTGT	14	0.35000000000000003	No Hit
GACAAAACCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATGGTGG	13	0.325	No Hit
CTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCT	13	0.325	No Hit
CTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTTCTTGCCG	13	0.325	No Hit
CTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCCTGTCAACTTC	13	0.325	No Hit
GTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCA	12	0.3	No Hit
GCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCC	12	0.3	No Hit
GCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTC	12	0.3	No Hit
GTGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATG	11	0.27499999999999997	No Hit
CTGAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACT	11	0.27499999999999997	No Hit
CGGGTGTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAG	11	0.27499999999999997	No Hit
GGAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACT	10	0.25	No Hit
CTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTG	10	0.25	No Hit
CGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCTGCAGACCACGTCGAAG	10	0.25	No Hit
TGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTC	10	0.25	No Hit
AGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATT	10	0.25	No Hit
CTCGGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCAT	10	0.25	No Hit
GGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACT	10	0.25	No Hit
GTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGA	10	0.25	No Hit
TGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGAC	9	0.22499999999999998	No Hit
CTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGT	9	0.22499999999999998	No Hit
CAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGT	9	0.22499999999999998	No Hit
GCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGAT	9	0.22499999999999998	No Hit
CTGCAGACCACGTCGAAGACCTGCCTGGTGCCCTGTCCACTCTGAGCGAC	9	0.22499999999999998	No Hit
CCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACC	9	0.22499999999999998	No Hit
CTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCT	9	0.22499999999999998	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	9	0.22499999999999998	Illumina Single End PCR Primer 1 (100% over 50bp)
CCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTGCCCAGGTC	8	0.2	No Hit
GTGCACTCGGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACA	8	0.2	No Hit
CTAAGGTGAAGGCCCATGGCAAGAAGGTGATAAACGCCTTCAATGATGGC	8	0.2	No Hit
GTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTC	8	0.2	No Hit
AAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACTGC	8	0.2	No Hit
GTCCACTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCCTGTCA	8	0.2	No Hit
CTGGTTGTCTACCCTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCT	8	0.2	No Hit
GTTGTGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTG	8	0.2	No Hit
GTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGT	8	0.2	No Hit
CCACACGCAGTTTGTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCA	8	0.2	No Hit
AGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGTC	8	0.2	No Hit
GTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCT	8	0.2	No Hit
CTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAA	8	0.2	No Hit
GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATT	7	0.17500000000000002	No Hit
GCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACAGGATC	7	0.17500000000000002	No Hit
CTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATGG	7	0.17500000000000002	No Hit
CCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATGGTGGTGAATAT	7	0.17500000000000002	No Hit
GTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGG	7	0.17500000000000002	No Hit
CTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATC	7	0.17500000000000002	No Hit
CACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGCTGAGAAGG	7	0.17500000000000002	No Hit
CTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCC	7	0.17500000000000002	No Hit
CAGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGT	7	0.17500000000000002	No Hit
GGGGCTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGGGGAAGGCAG	7	0.17500000000000002	No Hit
GGGAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGC	7	0.17500000000000002	No Hit
CCTGCATGCCCACAAACTGCGTGTGGATCCTGTCAACTTCAAGTTCCTGA	7	0.17500000000000002	No Hit
CTTGGGAACAATTGACCATTGCACAAAGACAAGAGCAGGAAAAGAGGTTT	7	0.17500000000000002	No Hit
GTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACAGGATCCACACG	7	0.17500000000000002	No Hit
CGCAGTTTGTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAG	7	0.17500000000000002	No Hit
CCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTTAATGGCCTGTGG	7	0.17500000000000002	No Hit
TGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGAC	7	0.17500000000000002	No Hit
CGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCT	7	0.17500000000000002	No Hit
GAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCC	7	0.17500000000000002	No Hit
GGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTT	7	0.17500000000000002	No Hit
GTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAG	7	0.17500000000000002	No Hit
CAAAACCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATGGTGGTG	7	0.17500000000000002	No Hit
GTGCAAGGGAGGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGG	7	0.17500000000000002	No Hit
GAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCA	7	0.17500000000000002	No Hit
CTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAA	7	0.17500000000000002	No Hit
CCCGGCTCTGCCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTT	7	0.17500000000000002	No Hit
AAGAAGGTGATAAACGCCTTCAATGATGGCCTGAAACACTTGGACAACCT	6	0.15	No Hit
CTTTGCTCATCTGAGTGAACTCCACTGTGACAAGCTGCATGTGGATCCTG	6	0.15	No Hit
CGAAGACCTGCCTGGTGCCCTGTCCACTCTGAGCGACCTGCATGCCCACA	6	0.15	No Hit
CCTTGACCTGGGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGTAGGTC	6	0.15	No Hit
GCGGCTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAA	6	0.15	No Hit
GCTGGTTGTCTACCCTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACC	6	0.15	No Hit
CTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGG	6	0.15	No Hit
GGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCAC	6	0.15	No Hit
GGAATTTGTCCAGAGAGGCGTGCATGGCGGGTGTGAAATCTCCAGGGTGG	6	0.15	No Hit
ACAGGATCCACACGCAGTTTGTGGGCATGCAGGTCGCTCAGAGTGGACAG	6	0.15	No Hit
GGGGAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGA	6	0.15	No Hit
GAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCA	6	0.15	No Hit
CTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCTGCTATCATGGGTAACC	6	0.15	No Hit
CCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCTGCTATC	6	0.15	No Hit
GGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATTGAT	6	0.15	No Hit
GTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCAGCCACCACCTT	6	0.15	No Hit
GTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGA	6	0.15	No Hit
GCCTGAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAA	6	0.15	No Hit
GGGACCTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCC	6	0.15	No Hit
TGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGC	6	0.15	No Hit
CTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGTCGCTCA	6	0.15	No Hit
CCTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATG	5	0.125	No Hit
GGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTG	5	0.125	No Hit
CTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATGGCAAGAAGGTGATA	5	0.125	No Hit
GGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGT	5	0.125	No Hit
GTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCAT	5	0.125	No Hit
CTGGGGGAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTAC	5	0.125	No Hit
GGGGAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTG	5	0.125	No Hit
GGCTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAATT	5	0.125	No Hit
GCTCATCTGAGTGAACTCCACTGTGACAAGCTGCATGTGGATCCTGAGAA	5	0.125	No Hit
GTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATC	5	0.125	No Hit
AAAAGAGGTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCAGCCA	5	0.125	No Hit
CCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAA	5	0.125	No Hit
CCACTGCCTGCTGGTGACCTTGGCTTGCCACCACCCTGGAGATTTCACAC	5	0.125	No Hit
GGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTGACA	5	0.125	No Hit
CGTGCATGGCGGGTGTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACC	5	0.125	No Hit
CCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCT	5	0.125	No Hit
CCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGT	5	0.125	No Hit
CAAGAAGGTGATAAACGCCTTCAATGATGGCCTGAAACACTTGGACAACC	5	0.125	No Hit
GGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGC	5	0.125	No Hit
CTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGGGGAAGGCAGCGAA	5	0.125	No Hit
CAGATTTTCAAAGGTCTTTGTCATTTTGACAACAACTGACAGATGCTCTC	5	0.125	No Hit
GGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGAC	5	0.125	No Hit
CAGCACAGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGGCGTGCATGG	5	0.125	No Hit
TGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTT	5	0.125	No Hit
CTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTCCTGGG	5	0.125	No Hit
CATTGATGTAAGCCCCGGCTCTGCCCAGGTCAAGGCTCACGGCAAGAAGG	5	0.125	No Hit
GGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACG	5	0.125	No Hit
GTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTTCTTGCCGTGAGCCT	5	0.125	No Hit
GTCAGCACAGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGGCGTGCAT	5	0.125	No Hit
CACCACCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTGCCC	5	0.125	No Hit
GTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTAT	5	0.125	No Hit
CTCACGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCTGCAGACCACGTC	5	0.125	No Hit
CTTAACGGTACTTGGAGGTCAGCACGGTGCTCACAGAGGCAAGGAATTTG	5	0.125	No Hit
CTTTGGGGACCTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGA	5	0.125	No Hit
GGGAGGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTT	5	0.125	No Hit
CCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGAGGTACTTTGAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0125	0.0	0.0	0.0	0.0
26-27	0.025	0.0	0.0	0.0	0.0
28-29	0.025	0.0	0.0	0.0	0.0
30-31	0.05	0.0	0.0	0.0	0.0
32-33	0.075	0.0	0.0	0.0	0.0
34-35	0.075	0.0	0.0	0.0	0.0
36-37	0.075	0.0	0.0	0.0	0.0
38-39	0.0875	0.0	0.0	0.0	0.0
40-41	0.125	0.0	0.0	0.0	0.0
42-43	0.175	0.0	0.0	0.0	0.0
44-45	0.2	0.0	0.0	0.0	0.0
46-47	0.25	0.0	0.0	0.0	0.0
48-49	0.25	0.0	0.0	0.0	0.0
50-51	0.275	0.0	0.0	0.0	0.0
52-53	0.3625	0.0	0.0	0.0	0.0
54-55	0.3875	0.0	0.0	0.0	0.0
56-57	0.4	0.0	0.0	0.0	0.0
58-59	0.4	0.0	0.0	0.0	0.0
60-61	0.4375	0.0	0.0	0.0	0.0
62-63	0.4875	0.0	0.0	0.0	0.0
64-65	0.5	0.0	0.0	0.0	0.0
66-67	0.5375000000000001	0.0	0.0	0.0	0.0
68-69	0.5874999999999999	0.0	0.0	0.0	0.0
70-71	0.725	0.0	0.0	0.0	0.0
72-73	0.7625	0.0	0.0	0.0	0.0
74-75	0.85	0.0	0.0	0.0	0.0
76-77	0.95	0.0	0.0	0.0	0.0
78-79	1.025	0.0	0.0	0.0	0.0
80-81	1.1875	0.0	0.0	0.0	0.0
82-83	1.25	0.0	0.0	0.0	0.0
84-85	1.375	0.0	0.0	0.0	0.0
86-87	1.4874999999999998	0.0	0.0	0.0	0.0
88-89	1.625	0.0	0.0	0.0	0.0
90-91	1.7000000000000002	0.0	0.0	0.0	0.0
92-93	1.7999999999999998	0.0	0.0	0.0	0.0
94-95	1.9375	0.0	0.0	0.0	0.0
96-97	2.1375	0.0	0.0	0.0	0.0
98-99	2.3	0.0	0.0	0.0	0.0
100-101	2.425	0.0	0.0	0.0	0.0
102-103	2.675	0.0	0.0	0.0	0.0
104-105	3.0125	0.0	0.0	0.0	0.0
106-107	3.3125	0.0	0.0	0.0	0.0
108-109	3.55	0.0	0.0	0.0	0.0
110-111	3.85	0.0	0.0	0.0	0.0
112-113	4.1875	0.0	0.0	0.0	0.0
114-115	4.4625	0.0	0.0	0.0	0.0
116-117	4.8375	0.0	0.0	0.0	0.0
118-119	5.237500000000001	0.0	0.0	0.0	0.0
120-121	5.6625	0.0	0.0	0.0	0.0
122-123	6.025	0.0	0.0	0.0	0.0
124-125	6.612500000000001	0.0	0.0	0.0	0.0
126-127	7.1625	0.0	0.0	0.0	0.0
128-129	7.7125	0.0	0.0	0.0	0.0
130-131	8.25	0.0	0.0	0.0	0.0
132-133	8.8125	0.0	0.0	0.0	0.0
134-135	9.475000000000001	0.0	0.0	0.0	0.0
136-137	10.325	0.0	0.0	0.0	0.0
138	10.8	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 3038051 spots for ERR6357086.sra
Written 3038051 spots for ERR6357086.sra
Read 3038058 spots for ERR6357086.sra
Written 3038058 spots for ERR6357086.sra
Read 3038051 spots for ERR6357086.sra
Written 3038051 spots for ERR6357086.sra
Read 3038051 spots for ERR6357086.sra
Written 3038051 spots for ERR6357086.sra
Read 3038051 spots for ERR6357086.sra
Written 3038051 spots for ERR6357086.sra
Read 3038051 spots for ERR6357086.sra
Written 3038051 spots for ERR6357086.sra
Read 3038051 spots for ERR6357086.sra
Written 3038051 spots for ERR6357086.sra
Read 3038051 spots for ERR6357086.sra
Written 3038051 spots for ERR6357086.sra
SRR ids: ['ERR6357086.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_bfw04hg2
ERR6357086.sra spots: 24304415
blocks: [[1, 3038051], [3038052, 6076102], [6076103, 9114153], [9114154, 12152204], [12152205, 15190255], [15190256, 18228306], [18228307, 21266357], [21266358, 24304415]]
ERR6357086 file size 8166812
ERR6357086 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o ERR6357086 ERR6357086_1.fastq ERR6357086_2.fastq
Input file:	ERR6357086_1.fastq
Paired file:	ERR6357086_2.fastq
trimmed:	ERR6357086-trimmed-pair1.fastq, ERR6357086-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Mon Dec 29 01:34:04 2025 >> started

Mon Dec 29 01:34:59 2025 >> done (55.129s)
24304415 read pairs processed; of these:
    1999 ( 0.01%) short read pairs filtered out after trimming by size control
   87531 ( 0.36%) empty read pairs filtered out after trimming by size control
24214885 (99.63%) read pairs available; of these:
 4726518 (19.52%) trimmed read pairs available after processing
19488367 (80.48%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     277	  0.00%
 19	     283	  0.00%
 20	     278	  0.00%
 21	     394	  0.00%
 22	     430	  0.00%
 23	     457	  0.00%
 24	     539	  0.00%
 25	     593	  0.00%
 26	     658	  0.00%
 27	     818	  0.00%
 28	     865	  0.00%
 29	     871	  0.00%
 30	    1063	  0.00%
 31	    1092	  0.00%
 32	    1098	  0.00%
 33	    1307	  0.01%
 34	    1232	  0.01%
 35	    1368	  0.01%
 36	    1594	  0.01%
 37	    1605	  0.01%
 38	    1871	  0.01%
 39	    1761	  0.01%
 40	    2018	  0.01%
 41	    2310	  0.01%
 42	    2307	  0.01%
 43	    2641	  0.01%
 44	    2528	  0.01%
 45	    2466	  0.01%
 46	    2819	  0.01%
 47	    2845	  0.01%
 48	    3027	  0.01%
 49	    3629	  0.01%
 50	    3580	  0.01%
 51	    3946	  0.02%
 52	    3973	  0.02%
 53	    4037	  0.02%
 54	    3977	  0.02%
 55	    3847	  0.02%
 56	    4178	  0.02%
 57	    4692	  0.02%
 58	    4459	  0.02%
 59	    5035	  0.02%
 60	    4613	  0.02%
 61	    5451	  0.02%
 62	    5489	  0.02%
 63	    5860	  0.02%
 64	    5838	  0.02%
 65	    5954	  0.02%
 66	    6872	  0.03%
 67	    6669	  0.03%
 68	    8054	  0.03%
 69	    7063	  0.03%
 70	    7945	  0.03%
 71	    8026	  0.03%
 72	    8458	  0.03%
 73	    9449	  0.04%
 74	    8771	  0.04%
 75	    8719	  0.04%
 76	    8895	  0.04%
 77	    8848	  0.04%
 78	    9951	  0.04%
 79	   10419	  0.04%
 80	   10256	  0.04%
 81	   11095	  0.05%
 82	   12876	  0.05%
 83	   13258	  0.05%
 84	   13523	  0.06%
 85	   13491	  0.06%
 86	   14211	  0.06%
 87	   15119	  0.06%
 88	   15734	  0.06%
 89	   17340	  0.07%
 90	   17522	  0.07%
 91	   18199	  0.08%
 92	   18730	  0.08%
 93	   22063	  0.09%
 94	   24073	  0.10%
 95	   25059	  0.10%
 96	   24843	  0.10%
 97	   22228	  0.09%
 98	   24211	  0.10%
 99	   24620	  0.10%
100	   25805	  0.11%
101	   25420	  0.10%
102	   27771	  0.11%
103	   34025	  0.14%
104	   34888	  0.14%
105	   39255	  0.16%
106	   32942	  0.14%
107	   36955	  0.15%
108	   36010	  0.15%
109	   41502	  0.17%
110	   41550	  0.17%
111	   41590	  0.17%
112	   39947	  0.16%
113	   43361	  0.18%
114	   51138	  0.21%
115	   52291	  0.22%
116	   48787	  0.20%
117	   52518	  0.22%
118	   49427	  0.20%
119	   51119	  0.21%
120	   48121	  0.20%
121	   55064	  0.23%
122	   53764	  0.22%
123	   59906	  0.25%
124	   64041	  0.26%
125	   64673	  0.27%
126	   67502	  0.28%
127	   61218	  0.25%
128	   77319	  0.32%
129	   61700	  0.25%
130	   71093	  0.29%
131	   74922	  0.31%
132	   82454	  0.34%
133	   75460	  0.31%
134	   79926	  0.33%
135	   96314	  0.40%
136	   94274	  0.39%
137	   94075	  0.39%
138	   96880	  0.40%
139	   93171	  0.38%
140	   91197	  0.38%
141	   96431	  0.40%
142	  105666	  0.44%
143	   95373	  0.39%
144	  117204	  0.48%
145	  110868	  0.46%
146	  132019	  0.55%
147	  148365	  0.61%
148	  202046	  0.83%
149	  750608	  3.10%
150	19488367	 80.48%
24214885 reads passed initial QC


criterion=sequence-density
sequence-density=5.17
sequence-density-rank=1
fanout-score=2.36
fanout-score-rank=41
prefix-density=5.09
prefix-fanout=2.4
sequence=GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA


criterion=fanout-score
sequence-density=0.60
sequence-density-rank=19
fanout-score=31.11
fanout-score-rank=1
prefix-density=9.37
prefix-fanout=2.0
sequence=GCAAGGAATTCTCCCCCTGTGCA


criterion=sequence-density
sequence-density=4.98
sequence-density-rank=1
fanout-score=2.37
fanout-score-rank=40
prefix-density=4.90
prefix-fanout=2.4
sequence=GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA


criterion=fanout-score
sequence-density=0.61
sequence-density-rank=18
fanout-score=29.88
fanout-score-rank=1
prefix-density=9.06
prefix-fanout=2.0
sequence=GCAAGGAATTCTCCCCCTGTGCA
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA -y GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA -o ERR6357086 ERR6357086_1.fastq ERR6357086_2.fastq
Input file:	ERR6357086_1.fastq
Paired file:	ERR6357086_2.fastq
trimmed:	ERR6357086-trimmed-pair1.fastq, ERR6357086-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGA
-- paired 3' end adapter sequence (-y):	GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Mon Dec 29 01:36:44 2025 >> started

Mon Dec 29 01:37:08 2025 >> done (24.670s)
16143257 read pairs processed; of these:
   10955 ( 0.07%) short read pairs filtered out after trimming by size control
  144491 ( 0.90%) empty read pairs filtered out after trimming by size control
15987811 (99.04%) read pairs available; of these:
    2232 ( 0.01%) trimmed read pairs available after processing
15985579 (99.99%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     190	  0.00%
 19	     183	  0.00%
 20	     175	  0.00%
 21	     259	  0.00%
 22	     278	  0.00%
 23	     291	  0.00%
 24	     359	  0.00%
 25	     406	  0.00%
 26	     445	  0.00%
 27	     532	  0.00%
 28	     590	  0.00%
 29	     568	  0.00%
 30	     683	  0.00%
 31	     718	  0.00%
 32	     746	  0.00%
 33	     846	  0.01%
 34	     810	  0.01%
 35	     904	  0.01%
 36	    1071	  0.01%
 37	    1040	  0.01%
 38	    1280	  0.01%
 39	    1134	  0.01%
 40	    1389	  0.01%
 41	    1528	  0.01%
 42	    1496	  0.01%
 43	    1724	  0.01%
 44	    1666	  0.01%
 45	    1592	  0.01%
 46	    1878	  0.01%
 47	    1896	  0.01%
 48	    2046	  0.01%
 49	    2421	  0.02%
 50	    2346	  0.01%
 51	    2602	  0.02%
 52	    2609	  0.02%
 53	    2569	  0.02%
 54	    2513	  0.02%
 55	    2540	  0.02%
 56	    2688	  0.02%
 57	    3104	  0.02%
 58	    3002	  0.02%
 59	    3304	  0.02%
 60	    3059	  0.02%
 61	    3647	  0.02%
 62	    3693	  0.02%
 63	    3944	  0.02%
 64	    3852	  0.02%
 65	    3894	  0.02%
 66	    4527	  0.03%
 67	    4415	  0.03%
 68	    5318	  0.03%
 69	    4679	  0.03%
 70	    5250	  0.03%
 71	    5221	  0.03%
 72	    5659	  0.04%
 73	    6316	  0.04%
 74	    5745	  0.04%
 75	    5868	  0.04%
 76	    5879	  0.04%
 77	    5907	  0.04%
 78	    6454	  0.04%
 79	    6926	  0.04%
 80	    6711	  0.04%
 81	    7349	  0.05%
 82	    8644	  0.05%
 83	    8678	  0.05%
 84	    9052	  0.06%
 85	    8935	  0.06%
 86	    9349	  0.06%
 87	    9971	  0.06%
 88	   10444	  0.07%
 89	   11549	  0.07%
 90	   11736	  0.07%
 91	   12077	  0.08%
 92	   12344	  0.08%
 93	   14600	  0.09%
 94	   16077	  0.10%
 95	   16537	  0.10%
 96	   16467	  0.10%
 97	   14901	  0.09%
 98	   16138	  0.10%
 99	   16242	  0.10%
100	   17018	  0.11%
101	   16785	  0.10%
102	   18413	  0.12%
103	   22669	  0.14%
104	   23127	  0.14%
105	   26056	  0.16%
106	   21854	  0.14%
107	   24584	  0.15%
108	   23924	  0.15%
109	   27729	  0.17%
110	   27642	  0.17%
111	   27725	  0.17%
112	   26392	  0.17%
113	   28943	  0.18%
114	   33891	  0.21%
115	   35017	  0.22%
116	   32485	  0.20%
117	   35023	  0.22%
118	   32574	  0.20%
119	   33758	  0.21%
120	   31819	  0.20%
121	   36714	  0.23%
122	   35882	  0.22%
123	   40125	  0.25%
124	   42483	  0.27%
125	   42776	  0.27%
126	   44800	  0.28%
127	   40519	  0.25%
128	   51466	  0.32%
129	   40432	  0.25%
130	   46450	  0.29%
131	   49092	  0.31%
132	   54752	  0.34%
133	   50019	  0.31%
134	   53305	  0.33%
135	   63790	  0.40%
136	   61978	  0.39%
137	   61638	  0.39%
138	   63707	  0.40%
139	   60816	  0.38%
140	   59506	  0.37%
141	   62746	  0.39%
142	   69565	  0.44%
143	   63110	  0.39%
144	   77644	  0.49%
145	   72712	  0.45%
146	   86419	  0.54%
147	   96826	  0.61%
148	  132883	  0.83%
149	  494380	  3.09%
150	12867373	 80.48%


criterion=sequence-density
sequence-density=5.06
sequence-density-rank=1
fanout-score=2.38
fanout-score-rank=41
prefix-density=5.09
prefix-fanout=2.4
sequence=GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA


criterion=fanout-score
sequence-density=0.59
sequence-density-rank=18
fanout-score=31.26
fanout-score-rank=1
prefix-density=9.37
prefix-fanout=2.0
sequence=GCAAGGAATTCTCCCCCTGTGCA


criterion=sequence-density
sequence-density=4.89
sequence-density-rank=1
fanout-score=2.39
fanout-score-rank=42
prefix-density=4.93
prefix-fanout=2.4
sequence=GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA


criterion=fanout-score
sequence-density=0.60
sequence-density-rank=18
fanout-score=30.36
fanout-score-rank=1
prefix-density=9.07
prefix-fanout=2.0
sequence=GCAAGGAATTCTCCCCCTGTGCA
ERR6357086 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Dec 29 01:38:00
                             Started mapping on |	Dec 29 01:38:00
                                    Finished on |	Dec 29 01:49:08
       Mapping speed, Million of reads per hour |	129.66

                          Number of input reads |	24059439
                      Average input read length |	290
                                    UNIQUE READS:
                   Uniquely mapped reads number |	18608525
                        Uniquely mapped reads % |	77.34%
                          Average mapped length |	289.33
                       Number of splices: Total |	23875406
            Number of splices: Annotated (sjdb) |	23412833
                       Number of splices: GT/AG |	23767657
                       Number of splices: GC/AG |	25363
                       Number of splices: AT/AC |	1919
               Number of splices: Non-canonical |	80467
                      Mismatch rate per base, % |	0.53%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.98
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.77
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	4686779
             % of reads mapped to multiple loci |	19.48%
        Number of reads mapped to too many loci |	54170
             % of reads mapped to too many loci |	0.23%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	2.93%
                     % of reads unmapped: other |	0.02%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	764135	764135	764135
N_multimapping	4686779	4686779	4686779
N_noFeature	360569	9388587	9497206
N_ambiguous	338591	124262	131337
UnstrandedReadsAssigned:17909365 PositiveStrandReadsAssigned:9095676 NegativeStrandReadsAssigned:8979982
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
ERR6357086 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 31,715
[index] number of k-mers: 49,915,185
[index] number of equivalence classes: 70,351
[quant] running in paired-end mode
[quant] will process pair 1: ERR6357086-trimmed-pair1.fastq
                             ERR6357086-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 24,059,439 reads, 19,508,707 reads pseudoaligned
[quant] estimated average fragment length: 199.38
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 946 rounds

  31716 ERR6357086.ke.tsv
  32883 ERR6357086.se.tsv
  64599 total
==> ERR6357086.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	756.66	2317	66.912
ENSRNOT00000040993.4	1039	840.62	1832	47.6217
ENSRNOT00000050156.3	1545	1346.62	18083	293.43
ENSRNOT00000043693.3	684	485.771	5196	233.731
ENSRNOT00000046201.3	204	48.7354	158	70.8422
ENSRNOT00000046108.3	681	482.771	5394	244.146
ENSRNOT00000049683.3	784	585.692	9013	336.263
ENSRNOT00000041241.3	348	152.535	250	35.8136
ENSRNOT00000044582.3	297	107.546	119	24.1786

==> ERR6357086.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	0
ENSRNOG00000058808	0
ENSRNOG00000061316	5
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	0
ERR6357086 completed mapping pipeline successfully
