Starting /dee2/code/volunteer_pipeline.sh ERR6357089
    current disk space = 190841140
    free memory = 194246480 
ERR6357089 SRAfilesize
2b8978a4da27d03ded582d38da805f1d  ERR6357089.sra
ERR6357089.sra file validated
ERR6357089 is paired end
ERR6357089 is conventional basespace
ERR6357089 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR6357089_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.54625	32.0	32.0	32.0	32.0	32.0
2	31.32125	32.0	32.0	32.0	32.0	32.0
3	35.31875	37.0	37.0	37.0	32.0	37.0
4	35.99625	37.0	37.0	37.0	32.0	37.0
5	36.40625	37.0	37.0	37.0	37.0	37.0
6	39.89125	41.0	41.0	41.0	37.0	41.0
7	40.01625	41.0	41.0	41.0	37.0	41.0
8	40.098	41.0	41.0	41.0	37.0	41.0
9	40.16775	41.0	41.0	41.0	37.0	41.0
10-14	40.10080000000001	41.0	41.0	41.0	37.0	41.0
15-19	39.991550000000004	41.0	41.0	41.0	37.0	41.0
20-24	39.76025	41.0	41.0	41.0	37.0	41.0
25-29	39.4091	41.0	41.0	41.0	37.0	41.0
30-34	39.1619	41.0	41.0	41.0	37.0	41.0
35-39	39.0003	41.0	41.0	41.0	37.0	41.0
40-44	38.84439999999999	41.0	41.0	41.0	36.0	41.0
45-49	38.52915	41.0	40.2	41.0	32.0	41.0
50-54	38.295100000000005	41.0	37.0	41.0	32.0	41.0
55-59	37.97045000000001	41.0	37.0	41.0	29.0	41.0
60-64	37.8099	41.0	37.0	41.0	27.0	41.0
65-69	37.50045	41.0	37.0	41.0	27.0	41.0
70-74	37.009550000000004	41.0	37.0	41.0	27.0	41.0
75-79	36.86365	41.0	37.0	41.0	27.0	41.0
80-84	37.2286	41.0	37.0	41.0	27.0	41.0
85-89	37.098699999999994	41.0	37.0	41.0	27.0	41.0
90-94	36.687200000000004	41.0	37.0	41.0	22.0	41.0
95-99	36.42530000000001	41.0	37.0	41.0	22.0	41.0
100-104	35.96695	41.0	35.0	41.0	22.0	41.0
105-109	35.61435	41.0	32.0	41.0	22.0	41.0
110-114	35.232000000000006	41.0	32.0	41.0	22.0	41.0
115-119	34.81355	41.0	32.0	41.0	20.0	41.0
120-124	34.3927	41.0	32.0	41.0	12.0	41.0
125-129	34.1502	40.2	32.0	41.0	12.0	41.0
130-134	33.568	37.0	28.0	41.0	12.0	41.0
135-139	32.83895	37.0	27.0	41.0	12.0	41.0
140-144	32.58055	37.0	26.0	41.0	12.0	41.0
145-149	31.89785	37.0	22.0	41.0	12.0	41.0
150	31.42	37.0	22.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	3.0
18	5.0
19	4.0
20	4.0
21	11.0
22	19.0
23	25.0
24	20.0
25	44.0
26	55.0
27	42.0
28	52.0
29	47.0
30	75.0
31	104.0
32	117.0
33	148.0
34	192.0
35	199.0
36	370.0
37	470.0
38	535.0
39	623.0
40	834.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.532649487115336	11.308481361020766	7.10532899674756	38.05354015511634
2	32.625	16.575	32.2	18.6
3	32.9	21.175	20.875	25.05
4	31.8	32.824999999999996	13.15	22.225
5	32.525	33.425	16.275000000000002	17.775
6	26.625	33.324999999999996	16.025	24.025
7	28.075	16.75	29.549999999999997	25.624999999999996
8	23.65	19.45	26.6	30.3
9	27.6	19.2	26.025	27.175
10-14	29.175	24.305	21.25	25.27
15-19	30.154999999999998	22.439999999999998	22.02	25.385
20-24	28.735	23.25	22.24	25.775
25-29	29.14	25.480000000000004	19.13	26.25
30-34	28.105000000000004	25.0	19.755	27.139999999999997
35-39	29.065	24.13	20.025000000000002	26.779999999999998
40-44	28.015	22.52	22.18	27.284999999999997
45-49	28.675	22.91	22.175	26.240000000000002
50-54	27.93	21.87	22.79	27.41
55-59	27.575	22.895	22.61	26.919999999999998
60-64	28.64	22.695	22.905	25.759999999999998
65-69	27.27636381819091	22.291114555727788	22.33111655582779	28.10140507025351
70-74	28.065	22.11	21.92	27.905
75-79	28.74	22.439999999999998	22.005	26.815
80-84	27.675	23.89	22.12	26.314999999999998
85-89	28.794999999999998	22.314999999999998	22.345000000000002	26.545
90-94	28.110000000000003	22.36	22.43	27.1
95-99	28.255000000000003	22.705000000000002	21.475	27.565
100-104	28.115000000000002	23.16	21.795	26.93
105-109	28.285	22.115000000000002	21.495	28.105000000000004
110-114	28.49	21.98	21.34	28.189999999999998
115-119	28.494999999999997	23.35	21.59	26.565
120-124	27.865000000000002	23.115	21.884999999999998	27.134999999999998
125-129	27.345000000000002	23.294999999999998	22.470000000000002	26.889999999999997
130-134	28.26	22.31	23.265	26.165
135-139	26.745	24.415	21.16	27.68
140-144	27.08	23.305	22.725	26.889999999999997
145-149	26.5	25.5	20.330000000000002	27.67
150	23.549999999999997	26.650000000000002	21.925	27.875
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.5
24	0.5
25	0.0
26	0.0
27	0.5
28	1.0
29	0.5
30	0.0
31	0.5
32	2.0
33	3.0
34	2.0
35	2.5
36	5.5
37	15.0
38	16.5
39	14.5
40	17.0
41	16.5
42	13.5
43	10.0
44	13.0
45	16.5
46	16.5
47	27.0
48	39.5
49	85.5
50	203.0
51	278.0
52	275.0
53	243.0
54	200.5
55	182.0
56	221.0
57	363.5
58	467.0
59	426.5
60	301.0
61	184.0
62	119.5
63	70.0
64	41.0
65	20.0
66	8.0
67	11.0
68	10.5
69	5.0
70	4.0
71	4.0
72	5.0
73	6.5
74	5.0
75	2.5
76	3.0
77	3.0
78	2.5
79	3.0
80	3.5
81	2.5
82	1.5
83	1.5
84	1.0
85	1.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.075
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.005
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.37482962289869	41.475
2	10.495229441163108	11.55
3	4.770558836892322	7.875
4	2.498864152657883	5.5
5	1.7719218537028625	4.875
6	1.3175829168559745	4.35
7	0.5452067242162654	2.1
8	0.7269422989550204	3.2
9	0.5452067242162654	2.7
>10	1.9536574284416175	16.375
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGG	34	0.8500000000000001	No Hit
GTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCC	31	0.775	No Hit
GTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCT	28	0.7000000000000001	No Hit
GTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGCT	24	0.6	No Hit
GGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCAC	23	0.575	No Hit
CTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTT	23	0.575	No Hit
GGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACA	23	0.575	No Hit
GGAAAAGAGGTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCAGC	20	0.5	No Hit
GTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACC	20	0.5	No Hit
GGAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCT	19	0.475	No Hit
CCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTGCCCAGGTC	17	0.42500000000000004	No Hit
TGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGAC	17	0.42500000000000004	No Hit
GGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACGG	16	0.4	No Hit
GACAAAACCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATGGTGG	15	0.375	No Hit
CTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCT	15	0.375	No Hit
GGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGG	15	0.375	No Hit
CTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATGG	14	0.35000000000000003	No Hit
GTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCAT	14	0.35000000000000003	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC	14	0.35000000000000003	TruSeq Adapter, Index 9 (100% over 50bp)
CTTGGGAACAATTGACCATTGCACAAAGACAAGAGCAGGAAAAGAGGTTT	14	0.35000000000000003	No Hit
CAGGAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAA	14	0.35000000000000003	No Hit
CGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCT	14	0.35000000000000003	No Hit
GCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCC	13	0.325	No Hit
GTTGTGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTG	13	0.325	No Hit
CTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAATTTG	12	0.3	No Hit
GCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTC	12	0.3	No Hit
CCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGC	12	0.3	No Hit
GGAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACT	11	0.27499999999999997	No Hit
GTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCA	11	0.27499999999999997	No Hit
CTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTG	11	0.27499999999999997	No Hit
CACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGCTGAGAAGG	11	0.27499999999999997	No Hit
CAGGCAGTGGCTCAGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGT	11	0.27499999999999997	No Hit
GTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGT	11	0.27499999999999997	No Hit
CTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAA	11	0.27499999999999997	No Hit
CTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCT	11	0.27499999999999997	No Hit
GTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTGT	11	0.27499999999999997	No Hit
GTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATTGATGTAA	10	0.25	No Hit
GGTAACCCTAAGGTGAAGGCCCATGGCAAGAAGGTGATAAACGCCTTCAA	10	0.25	No Hit
GTGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATG	10	0.25	No Hit
AGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATT	10	0.25	No Hit
CTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTGACAAGCTGCA	10	0.25	No Hit
GGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGTCG	10	0.25	No Hit
TGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGC	10	0.25	No Hit
GGGGACCTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGC	9	0.22499999999999998	No Hit
CAGGAAGCAATCATGGTGCTCTCTGCAGCTGACAAAACCAACATCAAGAA	9	0.22499999999999998	No Hit
GGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTG	9	0.22499999999999998	No Hit
AAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCA	9	0.22499999999999998	No Hit
CGGGTGTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAG	9	0.22499999999999998	No Hit
GAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCC	9	0.22499999999999998	No Hit
GGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTT	9	0.22499999999999998	No Hit
GGGGAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGA	9	0.22499999999999998	No Hit
CTCGGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCAT	9	0.22499999999999998	No Hit
GAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCA	9	0.22499999999999998	No Hit
GGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATTGAT	9	0.22499999999999998	No Hit
CCCGGCTCTGCCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTT	9	0.22499999999999998	No Hit
GTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGG	8	0.2	No Hit
GAACAATTGACCATTGCACAAAGACAAGAGCAGGAAAAGAGGTTTAGTGG	8	0.2	No Hit
CCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAA	8	0.2	No Hit
AAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACTGC	8	0.2	No Hit
GCGGGTGTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCA	8	0.2	No Hit
GGGAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGC	8	0.2	No Hit
CGCAGTTTGTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAG	8	0.2	No Hit
GCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGG	8	0.2	No Hit
GGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACG	8	0.2	No Hit
GTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGA	8	0.2	No Hit
GCCCTGTCCACTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCC	8	0.2	No Hit
GTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCT	8	0.2	No Hit
GGGACCTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCC	8	0.2	No Hit
GTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGA	8	0.2	No Hit
GGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGC	8	0.2	No Hit
GTCAACTTCAAGTTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTTGCCA	8	0.2	No Hit
GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATT	7	0.17500000000000002	No Hit
GCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACAGGATC	7	0.17500000000000002	No Hit
CTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCC	7	0.17500000000000002	No Hit
GTCCACTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCCTGTCA	7	0.17500000000000002	No Hit
GGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGC	7	0.17500000000000002	No Hit
ACAGGATCCACACGCAGTTTGTGGGCATGCAGGTCGCTCAGAGTGGACAG	7	0.17500000000000002	No Hit
CCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCT	7	0.17500000000000002	No Hit
CTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCCTGTCAACTTC	7	0.17500000000000002	No Hit
GTGAGCCTTGACCTGGGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGT	7	0.17500000000000002	No Hit
GTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTAT	7	0.17500000000000002	No Hit
GGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACT	7	0.17500000000000002	No Hit
CTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATGGCAAGA	7	0.17500000000000002	No Hit
CAGGAGGCGGCTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGC	6	0.15	No Hit
CTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGT	6	0.15	No Hit
CTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATGGCAAGAAGGTGATA	6	0.15	No Hit
CAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACAGGATCCACACGCAGTT	6	0.15	No Hit
AACGCCTTCAATGATGGCCTGAAACACTTGGACAACCTCAAGGGCACCTT	6	0.15	No Hit
CGAAGACCTGCCTGGTGCCCTGTCCACTCTGAGCGACCTGCATGCCCACA	6	0.15	No Hit
GGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACG	6	0.15	No Hit
GTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTC	6	0.15	No Hit
CCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTGTCTACC	6	0.15	No Hit
CGGGGCTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGGGGAAGGCA	6	0.15	No Hit
CTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTTCTTGCCG	6	0.15	No Hit
GGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTG	6	0.15	No Hit
GCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGAT	6	0.15	No Hit
CATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGG	6	0.15	No Hit
GTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACAGGATCCACACG	6	0.15	No Hit
CGGCTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAAT	6	0.15	No Hit
GGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGC	6	0.15	No Hit
CTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGGGGAAGGCAGCGAA	6	0.15	No Hit
GGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGAC	6	0.15	No Hit
CTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTCCTGGG	6	0.15	No Hit
CCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCTGCTATC	6	0.15	No Hit
GTGCAAGGGAGGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGG	6	0.15	No Hit
AGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGTC	6	0.15	No Hit
TCCACACGCAGTTTGTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACC	6	0.15	No Hit
CCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACC	6	0.15	No Hit
CTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAA	6	0.15	No Hit
GCCTTCCCCACCACCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGG	6	0.15	No Hit
CAGAGAGGCGTGCATGGCGGGTGTGAAATCTCCAGGGTGGTGGCAAGCCA	6	0.15	No Hit
GAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACTG	6	0.15	No Hit
GTGGATCCTGAGAACTTCAGGCTCCTGGGCAATATGATTGTGATTGTGTT	5	0.125	No Hit
AAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACGGTA	5	0.125	No Hit
GCCTTGGCCAAAGCTGCAGACCACGTCGAAGACCTGCCTGGTGCCCTGTC	5	0.125	No Hit
CTGCAGATGACAAAACCAACATCAAGAACTGCTGGGGGAAGATTGGTGGC	5	0.125	No Hit
CGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCTGCAGACCACGTCGAAG	5	0.125	No Hit
CCACAAACTGCGTGTGGATCCTGTCAACTTCAAGTTCCTGAGCCACTGCC	5	0.125	No Hit
CCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATGGTGGTGAATAT	5	0.125	No Hit
CTTGTGAGCCAGGGCACTGGCCACTCCAGCCACCACCTTCTGGAAGGCAG	5	0.125	No Hit
GAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATTG	5	0.125	No Hit
CTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATC	5	0.125	No Hit
CTGGGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGTAGGTCTTGGTGG	5	0.125	No Hit
GCGGCTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAA	5	0.125	No Hit
GAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTG	5	0.125	No Hit
CCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTC	5	0.125	No Hit
CTGAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACT	5	0.125	No Hit
GAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACGGT	5	0.125	No Hit
CTTGACCTGGGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGTAGGTCT	5	0.125	No Hit
CCACCACCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTGCC	5	0.125	No Hit
GGGGCTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGGGGAAGGCAG	5	0.125	No Hit
TGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTC	5	0.125	No Hit
CGTGCATGGCGGGTGTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACC	5	0.125	No Hit
CCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTT	5	0.125	No Hit
GTGAAAGTAAATGCCTTTTATTAGTAGGACAGATTTTCAAAGGTCTTTGT	5	0.125	No Hit
GGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCA	5	0.125	No Hit
CTCTCACATTGATGTAAGCCCCGGCTCTGCCCAGGTCAAGGCTCACGGCA	5	0.125	No Hit
GAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCA	5	0.125	No Hit
CTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCTGCTATCATGGGTAACC	5	0.125	No Hit
CTCAGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAG	5	0.125	No Hit
CCGGCTCTGCCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTTG	5	0.125	No Hit
CTGGTGCCCTGTCCACTCTGAGCGACCTGCATGCCCACAAACTGCGTGTG	5	0.125	No Hit
AGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTC	5	0.125	No Hit
GTAAGCCCCGGCTCTGCCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGA	5	0.125	No Hit
TGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTGTC	5	0.125	No Hit
CGGCAGGAGGCGGCTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGA	5	0.125	No Hit
CTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCA	5	0.125	No Hit
CCTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTCTGCCTC	5	0.125	No Hit
GTGCTCTCTGCAGATGACAAAACCAACATCAAGAACTGCTGGGGGAAGAT	5	0.125	No Hit
CTTGGAGGTCAGCACGGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGG	5	0.125	No Hit
CAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.025	0.0	0.0	0.0	0.0
26-27	0.025	0.0	0.0	0.0	0.0
28-29	0.025	0.0	0.0	0.0	0.0
30-31	0.037500000000000006	0.0	0.0	0.0	0.0
32-33	0.075	0.0	0.0	0.0	0.0
34-35	0.0875	0.0	0.0	0.0	0.0
36-37	0.1	0.0	0.0	0.0	0.0
38-39	0.125	0.0	0.0	0.0	0.0
40-41	0.175	0.0	0.0	0.0	0.0
42-43	0.225	0.0	0.0	0.0	0.0
44-45	0.225	0.0	0.0	0.0	0.0
46-47	0.225	0.0	0.0	0.0	0.0
48-49	0.2625	0.0	0.0	0.0	0.0
50-51	0.275	0.0	0.0	0.0	0.0
52-53	0.3125	0.0	0.0	0.0	0.0
54-55	0.35	0.0	0.0	0.0	0.0
56-57	0.35	0.0	0.0	0.0	0.0
58-59	0.35	0.0	0.0	0.0	0.0
60-61	0.35	0.0	0.0	0.0	0.0
62-63	0.375	0.0	0.0	0.0	0.0
64-65	0.4125	0.0	0.0	0.0	0.0
66-67	0.475	0.0	0.0	0.0	0.0
68-69	0.5375000000000001	0.0	0.0	0.0	0.0
70-71	0.5874999999999999	0.0	0.0	0.0	0.0
72-73	0.625	0.0	0.0	0.0	0.0
74-75	0.7375	0.0	0.0	0.0	0.0
76-77	0.8125	0.0	0.0	0.0	0.0
78-79	0.9	0.0	0.0	0.0	0.0
80-81	0.975	0.0	0.0	0.0	0.0
82-83	1.0875	0.0	0.0	0.0	0.0
84-85	1.175	0.0	0.0	0.0	0.0
86-87	1.2625	0.0	0.0	0.0	0.0
88-89	1.35	0.0	0.0	0.0	0.0
90-91	1.4249999999999998	0.0	0.0	0.0	0.0
92-93	1.5750000000000002	0.0	0.0	0.0	0.0
94-95	1.7375	0.0	0.0	0.0	0.0
96-97	1.8875	0.0	0.0	0.0	0.0
98-99	1.9874999999999998	0.0	0.0	0.0	0.0
100-101	2.125	0.0	0.0	0.0	0.0
102-103	2.2125	0.0	0.0	0.0	0.0
104-105	2.5250000000000004	0.0	0.0	0.0	0.0
106-107	2.825	0.0	0.0	0.0	0.0
108-109	3.0625	0.0	0.0	0.0	0.0
110-111	3.4000000000000004	0.0	0.0	0.0	0.0
112-113	3.675	0.0	0.0	0.0	0.0
114-115	3.9749999999999996	0.0	0.0	0.0	0.0
116-117	4.3375	0.0	0.0	0.0	0.0
118-119	4.7875	0.0	0.0	0.0	0.0
120-121	5.1125	0.0	0.0	0.0	0.0
122-123	5.5875	0.0	0.0	0.0	0.0
124-125	5.9375	0.0	0.0	0.0	0.0
126-127	6.449999999999999	0.0	0.0	0.0	0.0
128-129	6.987500000000001	0.0	0.0	0.0	0.0
130-131	7.5875	0.0	0.0	0.0	0.0
132-133	8.425	0.0	0.0	0.0	0.0
134-135	9.024999999999999	0.0	0.0	0.0	0.0
136-137	9.837499999999999	0.0	0.0	0.0	0.0
138	10.45	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
ERR6357089 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR6357089_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70125	32.0	32.0	32.0	32.0	32.0
2	31.6675	32.0	32.0	32.0	32.0	32.0
3	35.67875	37.0	37.0	37.0	32.0	37.0
4	36.2075	37.0	37.0	37.0	37.0	37.0
5	36.35625	37.0	37.0	37.0	37.0	37.0
6	39.94975	41.0	41.0	41.0	37.0	41.0
7	40.04575	41.0	41.0	41.0	37.0	41.0
8	40.08625	41.0	41.0	41.0	37.0	41.0
9	40.113	41.0	41.0	41.0	37.0	41.0
10-14	40.106550000000006	41.0	41.0	41.0	40.2	41.0
15-19	39.9742	41.0	41.0	41.0	37.8	41.0
20-24	39.86845	41.0	41.0	41.0	37.0	41.0
25-29	39.77615000000001	41.0	41.0	41.0	37.0	41.0
30-34	39.7295	41.0	41.0	41.0	37.0	41.0
35-39	39.55694999999999	41.0	41.0	41.0	37.0	41.0
40-44	39.459050000000005	41.0	41.0	41.0	37.0	41.0
45-49	39.3086	41.0	41.0	41.0	37.0	41.0
50-54	39.201350000000005	41.0	41.0	41.0	37.0	41.0
55-59	39.09075	41.0	41.0	41.0	37.0	41.0
60-64	38.954499999999996	41.0	41.0	41.0	37.0	41.0
65-69	38.766850000000005	41.0	41.0	41.0	33.0	41.0
70-74	38.5351	41.0	41.0	41.0	32.0	41.0
75-79	38.121700000000004	41.0	39.4	41.0	31.0	41.0
80-84	38.390699999999995	41.0	41.0	41.0	32.0	41.0
85-89	38.21895	41.0	41.0	41.0	32.0	41.0
90-94	38.0043	41.0	41.0	41.0	31.0	41.0
95-99	37.6431	41.0	39.4	41.0	27.0	41.0
100-104	37.3467	41.0	37.0	41.0	26.0	41.0
105-109	37.1421	41.0	37.0	41.0	22.0	41.0
110-114	36.89565	41.0	37.0	41.0	22.0	41.0
115-119	36.5774	41.0	37.0	41.0	22.0	41.0
120-124	36.223150000000004	41.0	37.0	41.0	22.0	41.0
125-129	35.89745	41.0	37.0	41.0	20.0	41.0
130-134	35.72015	41.0	37.0	41.0	14.0	41.0
135-139	35.2795	41.0	35.0	41.0	12.0	41.0
140-144	34.797250000000005	41.0	32.0	41.0	12.0	41.0
145-149	34.61635	41.0	32.0	41.0	12.0	41.0
150	34.31075	41.0	32.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	2.0
16	2.0
17	3.0
18	12.0
19	7.0
20	8.0
21	20.0
22	10.0
23	25.0
24	19.0
25	26.0
26	48.0
27	50.0
28	41.0
29	42.0
30	67.0
31	52.0
32	76.0
33	83.0
34	89.0
35	111.0
36	151.0
37	206.0
38	292.0
39	672.0
40	1884.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.46011502875719	10.577644411102776	8.002000500125032	40.960240060015
2	32.16608304152076	16.65832916458229	31.79089544772386	19.384692346173086
3	33.10827706926732	21.73043260815204	18.97974493623406	26.18154538634659
4	31.532883220805203	31.707926981745437	14.47861965491373	22.280570142535634
5	34.28357089272318	31.932983245811453	15.55388847211803	18.229557389347338
6	26.85671417854464	33.158289572393095	16.179044761190298	23.80595148787197
7	28.35708927231808	17.27931982995749	30.23255813953488	24.131032758189548
8	23.06153076538269	20.460230115057527	25.062531265632813	31.41570785392696
9	26.413206603301653	21.010505252626313	25.18759379689845	27.388694347173587
10-14	30.23407022106632	23.48704611383415	21.456436931079324	24.822446734020208
15-19	30.257102841136458	22.393957583033213	22.058823529411764	25.290116046418564
20-24	28.74362308692608	22.7518255476643	22.471741522456735	26.032809842952886
25-29	28.74362308692608	25.07752325697709	19.86095828748625	26.317895368610582
30-34	27.76749537291781	24.38597368815967	20.039017557901058	27.807513381021458
35-39	29.451780712284915	24.034613845538217	19.962985194077632	26.55062024809924
40-44	28.24206051512878	22.77069267316829	21.555388847211805	27.431857964491122
45-49	27.618285485645693	22.381714514354307	22.48174452335701	27.518255476642995
50-54	27.39458810583704	21.877657180013006	23.36817886260191	27.35957585154804
55-59	27.9183755126538	23.186956086826047	22.12663799139742	26.76803040912274
60-64	28.306322529011606	22.95918367346939	22.759103641456583	25.97539015606242
65-69	27.239533836842895	21.63257139999	22.19776921922673	28.930125543940377
70-74	28.104836692842493	21.767618666533288	21.732606412244284	28.394938228379935
75-79	28.174087043521762	22.23111555777889	22.521260630315158	27.073536768384194
80-84	27.699694893212623	23.108087830740757	22.702946031110887	26.489271244935725
85-89	28.566426570628252	22.358943577430974	22.27891156462585	26.795718287314923
90-94	27.808904452226113	22.65632816408204	21.5807903951976	27.953976988494244
95-99	27.963981990995496	22.926463231615806	21.070535267633815	28.039019509754876
100-104	27.972587664449	23.435545995698064	21.994897703966785	26.59696863588615
105-109	28.60501175411394	21.172410343620268	22.627919771920173	27.59465813034562
110-114	28.33775198839478	22.285028262718225	21.889850432694715	27.487369316192282
115-119	28.27489621367479	22.9830440654229	21.347471615065274	27.39458810583704
120-124	27.97758991546196	23.20044019808914	21.779800910409687	27.042168976039214
125-129	27.455982392957186	23.629451780712284	22.288915566226493	26.625650260104038
130-134	28.58000300105037	23.188115840544192	23.438203371179913	24.79367778722553
135-139	27.503751875937972	24.482241120560282	21.38569284642321	26.62831415707854
140-144	28.78651460584234	23.184273709483794	22.59903961584634	25.430172068827535
145-149	27.956182472989195	24.869947979191675	21.233493397358945	25.94037615046018
150	27.00675168792198	25.30632658164541	21.005251312828207	26.6816704176044
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	0.5
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	1.0
29	1.0
30	1.0
31	1.5
32	2.0
33	4.5
34	4.0
35	2.5
36	6.0
37	11.5
38	10.5
39	11.0
40	10.0
41	7.5
42	10.0
43	10.0
44	12.5
45	15.0
46	18.5
47	24.0
48	42.0
49	95.5
50	188.0
51	267.0
52	262.0
53	237.0
54	215.5
55	195.0
56	225.0
57	338.5
58	453.5
59	441.5
60	322.0
61	202.0
62	130.0
63	79.0
64	46.0
65	19.5
66	6.5
67	6.5
68	7.0
69	6.0
70	5.5
71	4.0
72	3.0
73	6.0
74	6.5
75	3.0
76	3.0
77	2.5
78	3.0
79	4.0
80	2.0
81	1.0
82	1.0
83	2.0
84	1.5
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.05
3	0.025
4	0.025
5	0.025
6	0.025
7	0.025
8	0.05
9	0.05
10-14	0.03
15-19	0.04
20-24	0.03
25-29	0.03
30-34	0.045
35-39	0.04
40-44	0.025
45-49	0.03
50-54	0.034999999999999996
55-59	0.03
60-64	0.04
65-69	0.034999999999999996
70-74	0.034999999999999996
75-79	0.05
80-84	0.034999999999999996
85-89	0.04
90-94	0.05
95-99	0.05
100-104	0.045
105-109	0.034999999999999996
110-114	0.045
115-119	0.034999999999999996
120-124	0.045
125-129	0.04
130-134	0.034999999999999996
135-139	0.05
140-144	0.04
145-149	0.04
150	0.025
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.925000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.78031937850669	44.474999999999994
2	9.667673716012084	11.200000000000001
3	4.14328873543375	7.199999999999999
4	3.2369443245576175	7.5
5	1.855848079413034	5.375
6	0.9495036685369012	3.3000000000000003
7	0.6473888649115235	2.625
8	0.8200258955545966	3.8
9	0.38843331894691413	2.025
>10	1.5105740181268883	12.5
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGG	32	0.8	No Hit
GGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACA	23	0.575	No Hit
GGAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCT	20	0.5	No Hit
GTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCT	19	0.475	No Hit
GTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCC	19	0.475	No Hit
GTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACC	19	0.475	No Hit
CTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCT	18	0.44999999999999996	No Hit
TGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGAC	16	0.4	No Hit
CTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTT	16	0.4	No Hit
GTCCACTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCCTGTCA	15	0.375	No Hit
CAGGAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAA	15	0.375	No Hit
GGAAAAGAGGTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCAGC	15	0.375	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	15	0.375	Illumina Single End PCR Primer 1 (100% over 50bp)
GTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCA	14	0.35000000000000003	No Hit
CTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTG	14	0.35000000000000003	No Hit
CTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCCTGTCAACTTC	14	0.35000000000000003	No Hit
GCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTC	14	0.35000000000000003	No Hit
GTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTGT	14	0.35000000000000003	No Hit
GGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACGG	12	0.3	No Hit
GTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGCT	12	0.3	No Hit
CAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGT	12	0.3	No Hit
CGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCT	12	0.3	No Hit
GGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTT	12	0.3	No Hit
CCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTGCCCAGGTC	11	0.27499999999999997	No Hit
GGAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACT	11	0.27499999999999997	No Hit
AGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATT	11	0.27499999999999997	No Hit
CTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTGACAAGCTGCA	11	0.27499999999999997	No Hit
GTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGA	11	0.27499999999999997	No Hit
CCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGC	11	0.27499999999999997	No Hit
CTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCA	11	0.27499999999999997	No Hit
CTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAA	11	0.27499999999999997	No Hit
GTCGAAGACCTGCCTGGTGCCCTGTCCACTCTGAGCGACCTGCATGCCCA	10	0.25	No Hit
GACAAAACCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATGGTGG	10	0.25	No Hit
AAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACTGC	10	0.25	No Hit
CCCGGCTCTGCCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTT	10	0.25	No Hit
CCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATGGTGGTGAATAT	9	0.22499999999999998	No Hit
CTTGGGAACAATTGACCATTGCACAAAGACAAGAGCAGGAAAAGAGGTTT	9	0.22499999999999998	No Hit
CCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCT	9	0.22499999999999998	No Hit
CTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCTGCTATCATGGGTAACC	9	0.22499999999999998	No Hit
GTGCAAGGGAGGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGG	9	0.22499999999999998	No Hit
GGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGG	9	0.22499999999999998	No Hit
AGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGTC	9	0.22499999999999998	No Hit
GGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACT	9	0.22499999999999998	No Hit
GTCAACTTCAAGTTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTTGCCA	9	0.22499999999999998	No Hit
GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATT	8	0.2	No Hit
GGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTG	8	0.2	No Hit
CACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGCTGAGAAGG	8	0.2	No Hit
CTAAGGTGAAGGCCCATGGCAAGAAGGTGATAAACGCCTTCAATGATGGC	8	0.2	No Hit
CTGAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACT	8	0.2	No Hit
CCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTGTCTACC	8	0.2	No Hit
CAGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGT	8	0.2	No Hit
CAGGCAGTGGCTCAGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGT	8	0.2	No Hit
GGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCAC	8	0.2	No Hit
TGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGAC	8	0.2	No Hit
CTTGGCCAAAGCTGCAGACCACGTCGAAGACCTGCCTGGTGCCCTGTCCA	8	0.2	No Hit
CTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGGGGAAGGCAGCGAA	8	0.2	No Hit
GCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTG	8	0.2	No Hit
CTGCAGACCACGTCGAAGACCTGCCTGGTGCCCTGTCCACTCTGAGCGAC	8	0.2	No Hit
GGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACG	8	0.2	No Hit
CCACACGCAGTTTGTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCA	8	0.2	No Hit
CTCACGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCTGCAGACCACGTC	8	0.2	No Hit
CTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAA	8	0.2	No Hit
CTTAACGGTACTTGGAGGTCAGCACGGTGCTCACAGAGGCAAGGAATTTG	8	0.2	No Hit
GGCGGCTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGA	7	0.17500000000000002	No Hit
GGTAACCCTAAGGTGAAGGCCCATGGCAAGAAGGTGATAAACGCCTTCAA	7	0.17500000000000002	No Hit
CTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATGG	7	0.17500000000000002	No Hit
GCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCC	7	0.17500000000000002	No Hit
GAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATTG	7	0.17500000000000002	No Hit
CTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATC	7	0.17500000000000002	No Hit
CGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCT	7	0.17500000000000002	No Hit
GGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTGACA	7	0.17500000000000002	No Hit
TGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTC	7	0.17500000000000002	No Hit
CACAAACTGCGTGTGGATCCTGTCAACTTCAAGTTCCTGAGCCACTGCCT	7	0.17500000000000002	No Hit
GTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCTGCAGA	7	0.17500000000000002	No Hit
CCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTTAATGGCCTGTGG	7	0.17500000000000002	No Hit
GTTGTGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTG	7	0.17500000000000002	No Hit
GAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCC	7	0.17500000000000002	No Hit
GAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCA	7	0.17500000000000002	No Hit
GGGGACCTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGC	6	0.15	No Hit
CTTGGGAACAATTGACCAATGCACAAAGACAAGAGCAGGAAAAGAGGTTT	6	0.15	No Hit
GCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACAGGATC	6	0.15	No Hit
GCTGGTTGTCTACCCTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACC	6	0.15	No Hit
GTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTC	6	0.15	No Hit
AAAAGAGGTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCAGCCA	6	0.15	No Hit
AAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCA	6	0.15	No Hit
CCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAA	6	0.15	No Hit
CTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTTCTTGCCG	6	0.15	No Hit
GCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGAT	6	0.15	No Hit
GTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTT	6	0.15	No Hit
GCTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAATTT	6	0.15	No Hit
GAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCA	6	0.15	No Hit
TGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTT	6	0.15	No Hit
CTCGGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCAT	6	0.15	No Hit
GTCAGCACAGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGGCGTGCAT	6	0.15	No Hit
GTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTAT	6	0.15	No Hit
GCCCTGTCCACTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCC	6	0.15	No Hit
CCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCC	6	0.15	No Hit
GTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCT	6	0.15	No Hit
AAGCAATCATGGTGCTCTCTGCAGCTGACAAAACCAACATCAAGAACTGC	6	0.15	No Hit
GGGAGGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTT	6	0.15	No Hit
CGCCTTCAATGATGGCCTGAAACACTTGGACAACCTCAAGGGCACCTTTG	5	0.125	No Hit
CGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGAGGTACT	5	0.125	No Hit
CGGCAAGAAGGTTGCTGATGCCCTGGCCAAAGCTGCAGACCACGTCGAAG	5	0.125	No Hit
CCCGGCTCTGCCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCCT	5	0.125	No Hit
CCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGAGGTACTTTGATA	5	0.125	No Hit
GTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCAT	5	0.125	No Hit
GGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACG	5	0.125	No Hit
CTGGGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGTAGGTCTTGGTGG	5	0.125	No Hit
GGGCAATATGATTGTGATTGTGTTGGGCCACCACCTGGGCAAGGAATTCA	5	0.125	No Hit
GTGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATG	5	0.125	No Hit
GGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGG	5	0.125	No Hit
CCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTC	5	0.125	No Hit
CACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACAT	5	0.125	No Hit
CCACCACCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTGCC	5	0.125	No Hit
ATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGT	5	0.125	No Hit
GGAATTTGTCCAGAGAGGCGTGCATGGCGGGTGTGAAATCTCCAGGGTGG	5	0.125	No Hit
GGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCT	5	0.125	No Hit
CCGGGGCTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGGGGAAGGC	5	0.125	No Hit
GGAAGCAATCATGGTGCTCTCTGCAGCTGACAAAACCAACATCAAGAACT	5	0.125	No Hit
CGGGTGTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAG	5	0.125	No Hit
GGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGC	5	0.125	No Hit
CGTGCATGGCGGGTGTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACC	5	0.125	No Hit
GGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCC	5	0.125	No Hit
CTGCAGCTTTGGCCAAGGCATCAGCAACCTTCTTGCCGTGAGCCTTGACC	5	0.125	No Hit
CTGGTTGTCTACCCTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCT	5	0.125	No Hit
CAAGAAGGTGATAAACGCCTTCAATGATGGCCTGAAACACTTGGACAACC	5	0.125	No Hit
GGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGTCG	5	0.125	No Hit
GGGGAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGA	5	0.125	No Hit
GTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAG	5	0.125	No Hit
GGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGAC	5	0.125	No Hit
CCCACCACCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTGC	5	0.125	No Hit
CTCTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATGGCAAGAAGGTGA	5	0.125	No Hit
GTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGT	5	0.125	No Hit
GTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCAGCCACCACCTT	5	0.125	No Hit
CTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGG	5	0.125	No Hit
CTGGTGCCCTGTCCACTCTGAGCGACCTGCATGCCCACAAACTGCGTGTG	5	0.125	No Hit
GGCGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGAGGTA	5	0.125	No Hit
CTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATGGCAAGA	5	0.125	No Hit
GGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGC	5	0.125	No Hit
CAGAGAGGCGTGCATGGCGGGTGTGAAATCTCCAGGGTGGTGGCAAGCCA	5	0.125	No Hit
CAGGAAAAGAGGTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCA	5	0.125	No Hit
CTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCT	5	0.125	No Hit
CTACCCTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTCTG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.025	0.0	0.0	0.0	0.0
26-27	0.025	0.0	0.0	0.0	0.0
28-29	0.025	0.0	0.0	0.0	0.0
30-31	0.05	0.0	0.0	0.0	0.0
32-33	0.1	0.0	0.0	0.0	0.0
34-35	0.1125	0.0	0.0	0.0	0.0
36-37	0.125	0.0	0.0	0.0	0.0
38-39	0.15	0.0	0.0	0.0	0.0
40-41	0.2	0.0	0.0	0.0	0.0
42-43	0.25	0.0	0.0	0.0	0.0
44-45	0.25	0.0	0.0	0.0	0.0
46-47	0.25	0.0	0.0	0.0	0.0
48-49	0.2875	0.0	0.0	0.0	0.0
50-51	0.3	0.0	0.0	0.0	0.0
52-53	0.3375	0.0	0.0	0.0	0.0
54-55	0.375	0.0	0.0	0.0	0.0
56-57	0.375	0.0	0.0	0.0	0.0
58-59	0.375	0.0	0.0	0.0	0.0
60-61	0.375	0.0	0.0	0.0	0.0
62-63	0.4	0.0	0.0	0.0	0.0
64-65	0.45	0.0	0.0	0.0	0.0
66-67	0.525	0.0	0.0	0.0	0.0
68-69	0.6125	0.0	0.0	0.0	0.0
70-71	0.6625000000000001	0.0	0.0	0.0	0.0
72-73	0.7	0.0	0.0	0.0	0.0
74-75	0.8125	0.0	0.0	0.0	0.0
76-77	0.8999999999999999	0.0	0.0	0.0	0.0
78-79	1.0	0.0	0.0	0.0	0.0
80-81	1.075	0.0	0.0	0.0	0.0
82-83	1.1875	0.0	0.0	0.0	0.0
84-85	1.325	0.0	0.0	0.0	0.0
86-87	1.4249999999999998	0.0	0.0	0.0	0.0
88-89	1.525	0.0	0.0	0.0	0.0
90-91	1.6	0.0	0.0	0.0	0.0
92-93	1.75	0.0	0.0	0.0	0.0
94-95	1.9125	0.0	0.0	0.0	0.0
96-97	2.0374999999999996	0.0	0.0	0.0	0.0
98-99	2.1375	0.0	0.0	0.0	0.0
100-101	2.275	0.0	0.0	0.0	0.0
102-103	2.3625	0.0	0.0	0.0	0.0
104-105	2.675	0.0	0.0	0.0	0.0
106-107	2.9749999999999996	0.0	0.0	0.0	0.0
108-109	3.1875	0.0	0.0	0.0	0.0
110-111	3.5	0.0	0.0	0.0	0.0
112-113	3.8125	0.0	0.0	0.0	0.0
114-115	4.1125	0.0	0.0	0.0	0.0
116-117	4.449999999999999	0.0	0.0	0.0	0.0
118-119	4.8875	0.0	0.0	0.0	0.0
120-121	5.2125	0.0	0.0	0.0	0.0
122-123	5.7125	0.0	0.0	0.0	0.0
124-125	6.074999999999999	0.0	0.0	0.0	0.0
126-127	6.5875	0.0	0.0	0.0	0.0
128-129	7.125	0.0	0.0	0.0	0.0
130-131	7.737500000000001	0.0	0.0	0.0	0.0
132-133	8.5625	0.0	0.0	0.0	0.0
134-135	9.1875	0.0	0.0	0.0	0.0
136-137	10.0	0.0	0.0	0.0	0.0
138	10.575	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACAATT	10	0.006973645	144.0	7
GAAGCAA	10	0.006973645	144.0	4
CAATTGA	10	0.006973645	144.0	9
AGGAAGC	10	0.006973645	144.0	2
CTTGGGA	10	0.006973645	144.0	1
GGAAGCA	10	0.006973645	144.0	3
GAACAAT	10	0.006973645	144.0	6
GGGAACA	10	0.006973645	144.0	4
CAGGAAG	10	0.006973645	144.0	1
ACAATTG	10	0.006973645	144.0	8
>>END_MODULE
Read 3049722 spots for ERR6357089.sra
Written 3049722 spots for ERR6357089.sra
Read 3049722 spots for ERR6357089.sra
Written 3049722 spots for ERR6357089.sra
Read 3049722 spots for ERR6357089.sra
Read 3049722 spots for ERR6357089.sra
Written 3049722 spots for ERR6357089.sra
Written 3049722 spots for ERR6357089.sra
Read 3049722 spots for ERR6357089.sra
Written 3049722 spots for ERR6357089.sra
Read 3049722 spots for ERR6357089.sra
Written 3049722 spots for ERR6357089.sra
Read 3049722 spots for ERR6357089.sra
Written 3049722 spots for ERR6357089.sra
Read 3049722 spots for ERR6357089.sra
Written 3049722 spots for ERR6357089.sra
SRR ids: ['ERR6357089.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_u3vmlvfv
ERR6357089.sra spots: 24397776
blocks: [[1, 3049722], [3049723, 6099444], [6099445, 9149166], [9149167, 12198888], [12198889, 15248610], [15248611, 18298332], [18298333, 21348054], [21348055, 24397776]]
ERR6357089 file size 8198264
ERR6357089 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o ERR6357089 ERR6357089_1.fastq ERR6357089_2.fastq
Input file:	ERR6357089_1.fastq
Paired file:	ERR6357089_2.fastq
trimmed:	ERR6357089-trimmed-pair1.fastq, ERR6357089-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Mon Dec 29 01:50:22 2025 >> started

Mon Dec 29 01:51:06 2025 >> done (43.401s)
24397776 read pairs processed; of these:
    2065 ( 0.01%) short read pairs filtered out after trimming by size control
   83169 ( 0.34%) empty read pairs filtered out after trimming by size control
24312542 (99.65%) read pairs available; of these:
 5071080 (20.86%) trimmed read pairs available after processing
19241462 (79.14%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     275	  0.00%
 19	     295	  0.00%
 20	     295	  0.00%
 21	     407	  0.00%
 22	     456	  0.00%
 23	     516	  0.00%
 24	     551	  0.00%
 25	     583	  0.00%
 26	     654	  0.00%
 27	     820	  0.00%
 28	     869	  0.00%
 29	     845	  0.00%
 30	    1103	  0.00%
 31	    1129	  0.00%
 32	    1201	  0.00%
 33	    1347	  0.01%
 34	    1249	  0.01%
 35	    1462	  0.01%
 36	    1630	  0.01%
 37	    1601	  0.01%
 38	    1950	  0.01%
 39	    1790	  0.01%
 40	    2070	  0.01%
 41	    2493	  0.01%
 42	    2470	  0.01%
 43	    2698	  0.01%
 44	    2652	  0.01%
 45	    2583	  0.01%
 46	    3032	  0.01%
 47	    3114	  0.01%
 48	    3223	  0.01%
 49	    3871	  0.02%
 50	    3805	  0.02%
 51	    4331	  0.02%
 52	    4363	  0.02%
 53	    4394	  0.02%
 54	    4328	  0.02%
 55	    4056	  0.02%
 56	    4385	  0.02%
 57	    5013	  0.02%
 58	    4768	  0.02%
 59	    5299	  0.02%
 60	    4954	  0.02%
 61	    5945	  0.02%
 62	    6077	  0.02%
 63	    6115	  0.03%
 64	    6280	  0.03%
 65	    6406	  0.03%
 66	    7327	  0.03%
 67	    7023	  0.03%
 68	    8528	  0.04%
 69	    7477	  0.03%
 70	    8372	  0.03%
 71	    8467	  0.03%
 72	    8966	  0.04%
 73	    9854	  0.04%
 74	    9310	  0.04%
 75	    9385	  0.04%
 76	    9367	  0.04%
 77	    9376	  0.04%
 78	   10885	  0.04%
 79	   10833	  0.04%
 80	   10994	  0.05%
 81	   11853	  0.05%
 82	   13914	  0.06%
 83	   13984	  0.06%
 84	   14251	  0.06%
 85	   14408	  0.06%
 86	   14951	  0.06%
 87	   15897	  0.07%
 88	   16736	  0.07%
 89	   18393	  0.08%
 90	   18280	  0.08%
 91	   19233	  0.08%
 92	   19850	  0.08%
 93	   22936	  0.09%
 94	   25711	  0.11%
 95	   26758	  0.11%
 96	   26503	  0.11%
 97	   24010	  0.10%
 98	   25925	  0.11%
 99	   26304	  0.11%
100	   27583	  0.11%
101	   26986	  0.11%
102	   29621	  0.12%
103	   36396	  0.15%
104	   37557	  0.15%
105	   42040	  0.17%
106	   35408	  0.15%
107	   39644	  0.16%
108	   39085	  0.16%
109	   45806	  0.19%
110	   45661	  0.19%
111	   45678	  0.19%
112	   43673	  0.18%
113	   47493	  0.20%
114	   56612	  0.23%
115	   57178	  0.24%
116	   53305	  0.22%
117	   58435	  0.24%
118	   54294	  0.22%
119	   57105	  0.23%
120	   53346	  0.22%
121	   60827	  0.25%
122	   59552	  0.24%
123	   66220	  0.27%
124	   71268	  0.29%
125	   71602	  0.29%
126	   74347	  0.31%
127	   67311	  0.28%
128	   85510	  0.35%
129	   68241	  0.28%
130	   78818	  0.32%
131	   82184	  0.34%
132	   90145	  0.37%
133	   83512	  0.34%
134	   88524	  0.36%
135	  105493	  0.43%
136	  103647	  0.43%
137	  103121	  0.42%
138	  105761	  0.44%
139	  101397	  0.42%
140	   99430	  0.41%
141	  105812	  0.44%
142	  115425	  0.47%
143	  103675	  0.43%
144	  127918	  0.53%
145	  119041	  0.49%
146	  143164	  0.59%
147	  157394	  0.65%
148	  210917	  0.87%
149	  752104	  3.09%
150	19241462	 79.14%
24312542 reads passed initial QC


criterion=sequence-density
sequence-density=5.13
sequence-density-rank=1
fanout-score=2.36
fanout-score-rank=41
prefix-density=5.05
prefix-fanout=2.4
sequence=GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAATGT


criterion=fanout-score
sequence-density=0.61
sequence-density-rank=18
fanout-score=30.35
fanout-score-rank=1
prefix-density=9.37
prefix-fanout=2.0
sequence=GCAAGGAATTCTCCCCCTGTGCA


criterion=sequence-density
sequence-density=4.97
sequence-density-rank=1
fanout-score=2.37
fanout-score-rank=41
prefix-density=4.89
prefix-fanout=2.4
sequence=GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA


criterion=fanout-score
sequence-density=0.61
sequence-density-rank=18
fanout-score=29.92
fanout-score-rank=1
prefix-density=9.06
prefix-fanout=2.0
sequence=GCAAGGAATTCTCCCCCTGTGCA
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAATGT -y GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA -o ERR6357089 ERR6357089_1.fastq ERR6357089_2.fastq
Input file:	ERR6357089_1.fastq
Paired file:	ERR6357089_2.fastq
trimmed:	ERR6357089-trimmed-pair1.fastq, ERR6357089-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGA
-- paired 3' end adapter sequence (-y):	GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Mon Dec 29 01:52:27 2025 >> started

Mon Dec 29 01:53:02 2025 >> done (35.196s)
16208361 read pairs processed; of these:
   10659 ( 0.07%) short read pairs filtered out after trimming by size control
  139876 ( 0.86%) empty read pairs filtered out after trimming by size control
16057826 (99.07%) read pairs available; of these:
    2474 ( 0.02%) trimmed read pairs available after processing
16055352 (99.98%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     203	  0.00%
 19	     195	  0.00%
 20	     185	  0.00%
 21	     238	  0.00%
 22	     300	  0.00%
 23	     340	  0.00%
 24	     377	  0.00%
 25	     389	  0.00%
 26	     431	  0.00%
 27	     547	  0.00%
 28	     571	  0.00%
 29	     549	  0.00%
 30	     755	  0.00%
 31	     732	  0.00%
 32	     785	  0.00%
 33	     904	  0.01%
 34	     801	  0.00%
 35	     978	  0.01%
 36	    1062	  0.01%
 37	    1092	  0.01%
 38	    1273	  0.01%
 39	    1152	  0.01%
 40	    1371	  0.01%
 41	    1625	  0.01%
 42	    1614	  0.01%
 43	    1767	  0.01%
 44	    1819	  0.01%
 45	    1689	  0.01%
 46	    2003	  0.01%
 47	    2117	  0.01%
 48	    2110	  0.01%
 49	    2575	  0.02%
 50	    2544	  0.02%
 51	    2929	  0.02%
 52	    2821	  0.02%
 53	    2758	  0.02%
 54	    2665	  0.02%
 55	    2631	  0.02%
 56	    2846	  0.02%
 57	    3319	  0.02%
 58	    3163	  0.02%
 59	    3460	  0.02%
 60	    3283	  0.02%
 61	    4073	  0.03%
 62	    4085	  0.03%
 63	    4025	  0.03%
 64	    4184	  0.03%
 65	    4248	  0.03%
 66	    4874	  0.03%
 67	    4740	  0.03%
 68	    5671	  0.04%
 69	    4957	  0.03%
 70	    5445	  0.03%
 71	    5514	  0.03%
 72	    5885	  0.04%
 73	    6563	  0.04%
 74	    6121	  0.04%
 75	    6244	  0.04%
 76	    6219	  0.04%
 77	    6147	  0.04%
 78	    7139	  0.04%
 79	    7057	  0.04%
 80	    7257	  0.05%
 81	    7854	  0.05%
 82	    9291	  0.06%
 83	    9381	  0.06%
 84	    9496	  0.06%
 85	    9521	  0.06%
 86	    9868	  0.06%
 87	   10502	  0.07%
 88	   11104	  0.07%
 89	   12204	  0.08%
 90	   12153	  0.08%
 91	   12857	  0.08%
 92	   13128	  0.08%
 93	   15243	  0.09%
 94	   17024	  0.11%
 95	   17839	  0.11%
 96	   17573	  0.11%
 97	   15939	  0.10%
 98	   17322	  0.11%
 99	   17343	  0.11%
100	   18240	  0.11%
101	   17843	  0.11%
102	   19629	  0.12%
103	   24255	  0.15%
104	   25006	  0.16%
105	   27914	  0.17%
106	   23571	  0.15%
107	   26359	  0.16%
108	   26175	  0.16%
109	   30426	  0.19%
110	   30426	  0.19%
111	   30355	  0.19%
112	   28968	  0.18%
113	   31721	  0.20%
114	   37588	  0.23%
115	   37797	  0.24%
116	   35591	  0.22%
117	   39033	  0.24%
118	   36071	  0.22%
119	   37681	  0.23%
120	   35383	  0.22%
121	   40458	  0.25%
122	   39542	  0.25%
123	   44261	  0.28%
124	   47510	  0.30%
125	   47508	  0.30%
126	   49176	  0.31%
127	   44953	  0.28%
128	   56965	  0.35%
129	   44852	  0.28%
130	   51492	  0.32%
131	   53932	  0.34%
132	   59993	  0.37%
133	   55262	  0.34%
134	   58869	  0.37%
135	   69899	  0.44%
136	   68179	  0.42%
137	   67820	  0.42%
138	   69491	  0.43%
139	   66209	  0.41%
140	   64985	  0.40%
141	   68715	  0.43%
142	   76269	  0.47%
143	   68762	  0.43%
144	   84771	  0.53%
145	   78260	  0.49%
146	   93702	  0.58%
147	  102662	  0.64%
148	  138634	  0.86%
149	  496055	  3.09%
150	12707550	 79.14%


criterion=sequence-density
sequence-density=4.94
sequence-density-rank=1
fanout-score=2.39
fanout-score-rank=40
prefix-density=4.98
prefix-fanout=2.4
sequence=GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA


criterion=fanout-score
sequence-density=0.56
sequence-density-rank=19
fanout-score=33.63
fanout-score-rank=1
prefix-density=9.60
prefix-fanout=2.0
sequence=GCAAGGAATTCTCCCCCTGTGCA


criterion=sequence-density
sequence-density=4.79
sequence-density-rank=1
fanout-score=2.42
fanout-score-rank=41
prefix-density=4.84
prefix-fanout=2.4
sequence=GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA


criterion=fanout-score
sequence-density=0.57
sequence-density-rank=18
fanout-score=32.52
fanout-score-rank=1
prefix-density=9.26
prefix-fanout=2.0
sequence=GCAAGGAATTCTCCCCCTGTGCA
ERR6357089 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Dec 29 01:53:46
                             Started mapping on |	Dec 29 01:53:46
                                    Finished on |	Dec 29 02:03:31
       Mapping speed, Million of reads per hour |	148.69

                          Number of input reads |	24162007
                      Average input read length |	290
                                    UNIQUE READS:
                   Uniquely mapped reads number |	18622172
                        Uniquely mapped reads % |	77.07%
                          Average mapped length |	288.87
                       Number of splices: Total |	23849712
            Number of splices: Annotated (sjdb) |	23380647
                       Number of splices: GT/AG |	23740858
                       Number of splices: GC/AG |	24574
                       Number of splices: AT/AC |	1922
               Number of splices: Non-canonical |	82358
                      Mismatch rate per base, % |	0.51%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.98
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.76
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	4795396
             % of reads mapped to multiple loci |	19.85%
        Number of reads mapped to too many loci |	56378
             % of reads mapped to too many loci |	0.23%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	2.83%
                     % of reads unmapped: other |	0.02%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	744439	744439	744439
N_multimapping	4795396	4795396	4795396
N_noFeature	339240	9391498	9487577
N_ambiguous	341972	126530	133419
UnstrandedReadsAssigned:17940960 PositiveStrandReadsAssigned:9104144 NegativeStrandReadsAssigned:9001176
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
ERR6357089 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 31,715
[index] number of k-mers: 49,915,185
[index] number of equivalence classes: 70,351
[quant] running in paired-end mode
[quant] will process pair 1: ERR6357089-trimmed-pair1.fastq
                             ERR6357089-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 24,162,007 reads, 19,674,785 reads pseudoaligned
[quant] estimated average fragment length: 196.812
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 984 rounds

  31716 ERR6357089.ke.tsv
  32883 ERR6357089.se.tsv
  64599 total
==> ERR6357089.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	759.188	2184	62.3616
ENSRNOT00000040993.4	1039	843.188	1694	43.5515
ENSRNOT00000050156.3	1545	1349.19	17039	273.77
ENSRNOT00000043693.3	684	488.336	4847	215.164
ENSRNOT00000046201.3	204	49.3111	148	65.0625
ENSRNOT00000046108.3	681	485.336	5289	236.236
ENSRNOT00000049683.3	784	588.313	8568	315.708
ENSRNOT00000041241.3	348	154.961	293	40.9883
ENSRNOT00000044582.3	297	109.681	108	21.3454

==> ERR6357089.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	0
ENSRNOG00000058808	0
ENSRNOG00000061316	5
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	0
ERR6357089 completed mapping pipeline successfully
