Starting /dee2/code/volunteer_pipeline.sh ERR6357088
    current disk space = 173751700
    free memory = 191159120 
ERR6357088 SRAfilesize
ddca8c51721fe10a49a2160a651487e1  ERR6357088.sra
ERR6357088.sra file validated
ERR6357088 is paired end
ERR6357088 is conventional basespace
ERR6357088 read1 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR6357088_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58125	32.0	32.0	32.0	32.0	32.0
2	31.3525	32.0	32.0	32.0	32.0	32.0
3	35.4475	37.0	37.0	37.0	32.0	37.0
4	36.025	37.0	37.0	37.0	32.0	37.0
5	36.36625	37.0	37.0	37.0	37.0	37.0
6	39.82725	41.0	41.0	41.0	37.0	41.0
7	39.97225	41.0	41.0	41.0	37.0	41.0
8	40.119	41.0	41.0	41.0	37.0	41.0
9	40.09875	41.0	41.0	41.0	37.0	41.0
10-14	40.0253	41.0	41.0	41.0	37.0	41.0
15-19	39.929899999999996	41.0	41.0	41.0	37.0	41.0
20-24	39.745050000000006	41.0	41.0	41.0	37.0	41.0
25-29	39.4424	41.0	41.0	41.0	37.0	41.0
30-34	39.1958	41.0	41.0	41.0	37.0	41.0
35-39	38.9774	41.0	41.0	41.0	37.0	41.0
40-44	38.77184999999999	41.0	41.0	41.0	33.0	41.0
45-49	38.509949999999996	41.0	39.4	41.0	32.0	41.0
50-54	38.3682	41.0	38.6	41.0	32.0	41.0
55-59	37.996950000000005	41.0	37.0	41.0	32.0	41.0
60-64	37.7155	41.0	37.0	41.0	27.0	41.0
65-69	37.370549999999994	41.0	37.0	41.0	27.0	41.0
70-74	36.95569999999999	41.0	37.0	41.0	27.0	41.0
75-79	36.94494999999999	41.0	37.0	41.0	27.0	41.0
80-84	37.28365	41.0	37.0	41.0	27.0	41.0
85-89	36.94535	41.0	37.0	41.0	24.0	41.0
90-94	36.68925	41.0	37.0	41.0	22.0	41.0
95-99	36.29415	41.0	37.0	41.0	22.0	41.0
100-104	35.952600000000004	41.0	36.0	41.0	22.0	41.0
105-109	35.6435	41.0	32.0	41.0	22.0	41.0
110-114	35.346149999999994	41.0	32.0	41.0	22.0	41.0
115-119	34.8297	41.0	32.0	41.0	16.0	41.0
120-124	34.5053	41.0	32.0	41.0	14.0	41.0
125-129	34.14575	41.0	32.0	41.0	12.0	41.0
130-134	33.6571	37.0	29.0	41.0	12.0	41.0
135-139	33.19545	37.0	27.0	41.0	12.0	41.0
140-144	32.60755	37.0	26.0	41.0	12.0	41.0
145-149	32.21445	37.0	24.0	41.0	12.0	41.0
150	31.76725	37.0	22.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	1.0
19	5.0
20	7.0
21	14.0
22	16.0
23	18.0
24	33.0
25	40.0
26	53.0
27	44.0
28	45.0
29	61.0
30	62.0
31	98.0
32	95.0
33	156.0
34	184.0
35	218.0
36	386.0
37	514.0
38	545.0
39	568.0
40	835.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.63565891472868	10.252563140785197	7.176794198549638	39.934983745936485
2	32.375	16.650000000000002	31.324999999999996	19.650000000000002
3	33.175	20.775	20.225	25.825
4	32.25	32.025	14.524999999999999	21.2
5	33.900000000000006	33.0	16.575	16.525000000000002
6	25.974999999999998	34.699999999999996	16.125	23.200000000000003
7	28.15	17.575	29.075	25.2
8	24.3	19.650000000000002	25.224999999999998	30.825000000000003
9	27.275	19.400000000000002	25.75	27.575
10-14	30.205	23.75	21.295	24.75
15-19	30.06	22.325	21.87	25.745
20-24	29.785	22.400000000000002	22.005	25.81
25-29	29.2	25.230000000000004	19.465	26.105
30-34	27.73	25.025	19.759999999999998	27.485
35-39	29.025000000000002	23.990000000000002	19.965	27.02
40-44	27.77	22.595000000000002	21.975	27.66
45-49	28.015	22.735	22.45	26.8
50-54	28.26	21.345	22.685	27.71
55-59	28.21	22.445	22.305	27.04
60-64	28.52	22.48	22.93	26.07
65-69	27.41	21.834999999999997	22.075	28.68
70-74	28.055000000000003	22.345000000000002	21.555	28.044999999999998
75-79	28.76	21.72	21.735	27.785
80-84	27.605	23.31	21.875	27.21
85-89	28.610000000000003	21.81	22.405	27.175
90-94	27.584999999999997	21.88	22.305	28.23
95-99	28.51	22.675	21.185000000000002	27.63
100-104	27.57	23.745	20.945	27.74
105-109	28.89	21.555	21.735	27.82
110-114	28.4	21.87	21.895	27.834999999999997
115-119	28.37	23.095	21.36	27.175
120-124	27.810000000000002	23.0	21.634999999999998	27.555000000000003
125-129	27.27	23.330000000000002	21.67	27.73
130-134	28.04	22.875	23.325000000000003	25.759999999999998
135-139	26.395000000000003	24.6	21.04	27.965
140-144	26.950000000000003	23.09	23.119999999999997	26.840000000000003
145-149	26.88	24.355	20.835	27.93
150	24.775	24.8	20.599999999999998	29.825000000000003
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.5
6	0.5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.5
27	0.5
28	0.5
29	1.0
30	0.5
31	1.0
32	1.5
33	0.5
34	2.5
35	5.0
36	9.0
37	14.0
38	13.0
39	14.0
40	14.5
41	9.0
42	8.5
43	11.0
44	13.0
45	14.0
46	12.0
47	16.5
48	33.0
49	86.0
50	169.0
51	234.0
52	244.5
53	211.0
54	196.0
55	178.5
56	223.5
57	393.0
58	496.5
59	476.5
60	336.0
61	196.0
62	138.5
63	78.0
64	41.5
65	21.0
66	10.0
67	6.5
68	6.5
69	7.0
70	6.0
71	5.0
72	6.0
73	5.5
74	3.5
75	1.5
76	2.5
77	3.5
78	2.5
79	3.5
80	2.5
81	1.5
82	3.0
83	2.5
84	1.5
85	1.5
86	0.5
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.19666820916243	40.625
2	9.94909763998149	10.75
3	5.043961129106895	8.175
4	2.5451180009254974	5.5
5	1.8509949097639982	5.0
6	1.3882461823229986	4.5
7	0.7866728366496992	2.9749999999999996
8	0.7866728366496992	3.4000000000000004
9	0.5090236001850995	2.475
>10	1.9435446552521982	16.6
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGG	45	1.125	No Hit
GTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCC	34	0.8500000000000001	No Hit
GTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCT	24	0.6	No Hit
GTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACC	24	0.6	No Hit
GGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACGG	22	0.5499999999999999	No Hit
GTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGCT	19	0.475	No Hit
GGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCAC	19	0.475	No Hit
CAGGAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAA	19	0.475	No Hit
CTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTT	18	0.44999999999999996	No Hit
GGAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCT	18	0.44999999999999996	No Hit
CCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTGCCCAGGTC	17	0.42500000000000004	No Hit
TGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGAC	17	0.42500000000000004	No Hit
CGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCTGCAGACCACGTCGAAG	17	0.42500000000000004	No Hit
GCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTC	17	0.42500000000000004	No Hit
GGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACA	17	0.42500000000000004	No Hit
GCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCC	16	0.4	No Hit
CGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCT	16	0.4	No Hit
GTCGAAGACCTGCCTGGTGCCCTGTCCACTCTGAGCGACCTGCATGCCCA	15	0.375	No Hit
GTGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATG	15	0.375	No Hit
CTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTTCTTGCCG	15	0.375	No Hit
GTCAACTTCAAGTTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTTGCCA	15	0.375	No Hit
GTCCACTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCCTGTCA	14	0.35000000000000003	No Hit
GGAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACT	13	0.325	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC	13	0.325	TruSeq Adapter, Index 7 (100% over 50bp)
GACAAAACCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATGGTGG	13	0.325	No Hit
CCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATGGTGGTGAATAT	13	0.325	No Hit
CTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCT	13	0.325	No Hit
CTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCTGCTATCATGGGTAACC	13	0.325	No Hit
GGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGG	13	0.325	No Hit
CTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCCTGTCAACTTC	12	0.3	No Hit
GGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTT	12	0.3	No Hit
GCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTG	12	0.3	No Hit
CCTTGACCTGGGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGTAGGTC	11	0.27499999999999997	No Hit
CAGGCAGTGGCTCAGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGT	11	0.27499999999999997	No Hit
GGAAAAGAGGTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCAGC	11	0.27499999999999997	No Hit
CCCGGCTCTGCCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTT	11	0.27499999999999997	No Hit
GCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACAGGATC	10	0.25	No Hit
GTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCA	10	0.25	No Hit
CGGGTGTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAG	10	0.25	No Hit
GCCCTGTCCACTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCC	10	0.25	No Hit
CCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGC	10	0.25	No Hit
CTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCA	10	0.25	No Hit
CTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGT	9	0.22499999999999998	No Hit
CTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATGG	9	0.22499999999999998	No Hit
CACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGCTGAGAAGG	9	0.22499999999999998	No Hit
AGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATC	9	0.22499999999999998	No Hit
CTTGACCTGGGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGTAGGTCT	9	0.22499999999999998	No Hit
CCTGCATGCCCACAAACTGCGTGTGGATCCTGTCAACTTCAAGTTCCTGA	9	0.22499999999999998	No Hit
CTTGGGAACAATTGACCATTGCACAAAGACAAGAGCAGGAAAAGAGGTTT	9	0.22499999999999998	No Hit
GTGGATCCTGTCAACTTCAAGTTCCTGAGCCACTGCCTGCTGGTGACCTT	9	0.22499999999999998	No Hit
GAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCC	9	0.22499999999999998	No Hit
GTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAG	9	0.22499999999999998	No Hit
CTCGGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCAT	9	0.22499999999999998	No Hit
GTGGATCCTGAGAACTTCAGGCTCCTGGGCAATATGATTGTGATTGTGTT	8	0.2	No Hit
CAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTTCT	8	0.2	No Hit
CCACAAACTGCGTGTGGATCCTGTCAACTTCAAGTTCCTGAGCCACTGCC	8	0.2	No Hit
GGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGT	8	0.2	No Hit
GTGCACTCGGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACA	8	0.2	No Hit
CTAAGGTGAAGGCCCATGGCAAGAAGGTGATAAACGCCTTCAATGATGGC	8	0.2	No Hit
CGGGGCTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGGGGAAGGCA	8	0.2	No Hit
CAGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGT	8	0.2	No Hit
CTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGG	8	0.2	No Hit
TGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGAC	8	0.2	No Hit
GTTGTGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTG	8	0.2	No Hit
CTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGGGGAAGGCAGCGAA	8	0.2	No Hit
GTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGA	8	0.2	No Hit
GGGTAACCCTAAGGTGAAGGCCCATGGCAAGAAGGTGATAAACGCCTTCA	8	0.2	No Hit
CCCATGGCAAGAAGGTGATAAACGCCTTCAATGATGGCCTGAAACACTTG	8	0.2	No Hit
TGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGC	8	0.2	No Hit
GTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTGT	8	0.2	No Hit
GGCGGCTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGA	7	0.17500000000000002	No Hit
CTGACTGATGCTGAGAAGGCTGCTGTTAATGGCCTGTGGGGAAAGGTGAA	7	0.17500000000000002	No Hit
GTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATTGATGTAA	7	0.17500000000000002	No Hit
CTGAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACT	7	0.17500000000000002	No Hit
AAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACTGC	7	0.17500000000000002	No Hit
CGCAGTTTGTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAG	7	0.17500000000000002	No Hit
AGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATT	7	0.17500000000000002	No Hit
GCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACC	7	0.17500000000000002	No Hit
GGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGTCG	7	0.17500000000000002	No Hit
GGGGAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGA	7	0.17500000000000002	No Hit
GAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCA	7	0.17500000000000002	No Hit
GTGCAAGGGAGGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGG	7	0.17500000000000002	No Hit
GGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACG	7	0.17500000000000002	No Hit
AGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGTC	7	0.17500000000000002	No Hit
GGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACT	7	0.17500000000000002	No Hit
CTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAA	7	0.17500000000000002	No Hit
CTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCT	7	0.17500000000000002	No Hit
CGGCAAGAAGGTTGCTGATGCCCTGGCCAAAGCTGCAGACCACGTCGAAG	6	0.15	No Hit
CAGGAAGCAATCATGGTGCTCTCTGCAGCTGACAAAACCAACATCAAGAA	6	0.15	No Hit
CTGCAGATGACAAAACCAACATCAAGAACTGCTGGGGGAAGATTGGTGGC	6	0.15	No Hit
CCTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATG	6	0.15	No Hit
GGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTG	6	0.15	No Hit
GGTAACCCTAAGGTGAAGGCCCATGGCAAGAAGGTGATAAACGCCTTCAA	6	0.15	No Hit
CGAAGACCTGCCTGGTGCCCTGTCCACTCTGAGCGACCTGCATGCCCACA	6	0.15	No Hit
AGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACGGTAC	6	0.15	No Hit
CTTGTGAGCCAGGGCACTGGCCACTCCAGCCACCACCTTCTGGAAGGCAG	6	0.15	No Hit
CCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCCTGGCCAAAGCT	6	0.15	No Hit
CAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGT	6	0.15	No Hit
GTCCAGAGAGGCGTGCATGGCGGGTGTGAAATCTCCAGGGTGGTGGCAAG	6	0.15	No Hit
CCTGTCCACTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCCTG	6	0.15	No Hit
GGAATTTGTCCAGAGAGGCGTGCATGGCGGGTGTGAAATCTCCAGGGTGG	6	0.15	No Hit
GCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGAT	6	0.15	No Hit
GGGAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGC	6	0.15	No Hit
GGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGC	6	0.15	No Hit
AGGAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAAC	6	0.15	No Hit
CTGGTTGTCTACCCTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCT	6	0.15	No Hit
CTGATGCCTTGGCCAAAGCTGCAGACCACGTCGAAGACCTGCCTGGTGCC	6	0.15	No Hit
GTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCAGCCACCACCTT	6	0.15	No Hit
GTCAGCACAGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGGCGTGCAT	6	0.15	No Hit
GGCGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGAGGTA	6	0.15	No Hit
GTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTAT	6	0.15	No Hit
GTCTACCCTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTC	6	0.15	No Hit
GTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCT	6	0.15	No Hit
CTTAACGGTACTTGGAGGTCAGCACGGTGCTCACAGAGGCAAGGAATTTG	6	0.15	No Hit
CAGGAAAAGAGGTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCA	6	0.15	No Hit
CTGCTGGTGACCTTGGCTTGCCACCACCCTGGAGATTTCACACCCGCCAT	6	0.15	No Hit
CTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGTCGCTCA	6	0.15	No Hit
GGGGACCTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGC	5	0.125	No Hit
CAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTG	5	0.125	No Hit
CCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTGCCCAGGTCAAGGCT	5	0.125	No Hit
CTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTG	5	0.125	No Hit
CTGGGGGAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTAC	5	0.125	No Hit
GTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGG	5	0.125	No Hit
CAAGAAGGTTGCTGATGCCTTGGCCAAAGCTGCAGACCACGTCGAAGACC	5	0.125	No Hit
GAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATTG	5	0.125	No Hit
CTTCTCTCACATTGATGTAAGCCCCGGCTCTGCCCAGGTCAAGGCTCACG	5	0.125	No Hit
GGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGG	5	0.125	No Hit
GGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCA	5	0.125	No Hit
CCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAA	5	0.125	No Hit
CTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAATTTG	5	0.125	No Hit
CCACCACCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTGCC	5	0.125	No Hit
GCGGGTGTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCA	5	0.125	No Hit
ATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGT	5	0.125	No Hit
GCCGGGGCTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGGGGAAGG	5	0.125	No Hit
CACAAACTGCGTGTGGATCCTGTCAACTTCAAGTTCCTGAGCCACTGCCT	5	0.125	No Hit
GTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCTGCAGA	5	0.125	No Hit
CCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCT	5	0.125	No Hit
CCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTT	5	0.125	No Hit
GCTTAACGGTACTTGGAGGTCAGCACGGTGCTCACAGAGGCAAGGAATTT	5	0.125	No Hit
CTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTGACAAGCTGCA	5	0.125	No Hit
GTGAGCCTTGACCTGGGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGT	5	0.125	No Hit
CAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACTGCTGG	5	0.125	No Hit
GGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGC	5	0.125	No Hit
CAGCACAGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGGCGTGCATGG	5	0.125	No Hit
TGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTT	5	0.125	No Hit
CCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCTGCTATC	5	0.125	No Hit
CCCACCACCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTGC	5	0.125	No Hit
AAGTAAATGCCTTTTATTAGTAGGACAGATTTTCAAAGGTCTTTGTCATT	5	0.125	No Hit
TCCACACGCAGTTTGTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACC	5	0.125	No Hit
GTGAACTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTC	5	0.125	No Hit
CTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATGGCAAGA	5	0.125	No Hit
GGGACCTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCC	5	0.125	No Hit
CAGACACCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTTAATGGC	5	0.125	No Hit
GTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGA	5	0.125	No Hit
GGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGC	5	0.125	No Hit
CTGCCATGCACGCCTCTCTGGACAAATTCCTTGCCTCTGTGAGCACCGTG	5	0.125	No Hit
GTGATAAACGCCTTCAATGATGGCCTGAAACACTTGGACAACCTCAAGGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0125	0.0	0.0	0.0	0.0
32-33	0.025	0.0	0.0	0.0	0.0
34-35	0.025	0.0	0.0	0.0	0.0
36-37	0.037500000000000006	0.0	0.0	0.0	0.0
38-39	0.1	0.0	0.0	0.0	0.0
40-41	0.1375	0.0	0.0	0.0	0.0
42-43	0.175	0.0	0.0	0.0	0.0
44-45	0.225	0.0	0.0	0.0	0.0
46-47	0.2625	0.0	0.0	0.0	0.0
48-49	0.3	0.0	0.0	0.0	0.0
50-51	0.3125	0.0	0.0	0.0	0.0
52-53	0.375	0.0	0.0	0.0	0.0
54-55	0.4	0.0	0.0	0.0	0.0
56-57	0.425	0.0	0.0	0.0	0.0
58-59	0.425	0.0	0.0	0.0	0.0
60-61	0.4625	0.0	0.0	0.0	0.0
62-63	0.5	0.0	0.0	0.0	0.0
64-65	0.6	0.0	0.0	0.0	0.0
66-67	0.6875	0.0	0.0	0.0	0.0
68-69	0.75	0.0	0.0	0.0	0.0
70-71	0.775	0.0	0.0	0.0	0.0
72-73	0.8125	0.0	0.0	0.0	0.0
74-75	0.9	0.0	0.0	0.0	0.0
76-77	1.0375	0.0	0.0	0.0	0.0
78-79	1.1625	0.0	0.0	0.0	0.0
80-81	1.2625000000000002	0.0	0.0	0.0	0.0
82-83	1.3125	0.0	0.0	0.0	0.0
84-85	1.4	0.0	0.0	0.0	0.0
86-87	1.55	0.0	0.0	0.0	0.0
88-89	1.7000000000000002	0.0	0.0	0.0	0.0
90-91	1.7999999999999998	0.0	0.0	0.0	0.0
92-93	1.9875	0.0	0.0	0.0	0.0
94-95	2.1875	0.0	0.0	0.0	0.0
96-97	2.3875	0.0	0.0	0.0	0.0
98-99	2.5375	0.0	0.0	0.0	0.0
100-101	2.8875	0.0	0.0	0.0	0.0
102-103	3.2	0.0	0.0	0.0	0.0
104-105	3.5125	0.0	0.0	0.0	0.0
106-107	3.8625	0.0	0.0	0.0	0.0
108-109	4.300000000000001	0.0	0.0	0.0	0.0
110-111	4.7875	0.0	0.0	0.0	0.0
112-113	5.275	0.0	0.0	0.0	0.0
114-115	5.737500000000001	0.0	0.0	0.0	0.0
116-117	6.25	0.0	0.0	0.0	0.0
118-119	6.5875	0.0	0.0	0.0	0.0
120-121	7.175	0.0	0.0	0.0	0.0
122-123	7.725	0.0	0.0	0.0	0.0
124-125	8.287500000000001	0.0	0.0	0.0	0.0
126-127	9.025	0.0	0.0	0.0	0.0
128-129	9.5625	0.0	0.0	0.0	0.0
130-131	10.0625	0.0	0.0	0.0	0.0
132-133	10.7375	0.0	0.0	0.0	0.0
134-135	11.4625	0.0	0.0	0.0	0.0
136-137	12.45	0.0	0.0	0.0	0.0
138	13.2	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACAATT	10	0.006973645	144.0	7
CAATTGA	10	0.006973645	144.0	9
TTGGGAA	10	0.006973645	144.0	2
GAACAAT	10	0.006973645	144.0	6
TGGGAAC	10	0.006973645	144.0	3
GGAACAA	10	0.006973645	144.0	5
ACAATTG	10	0.006973645	144.0	8
>>END_MODULE
ERR6357088 read2 length is 150 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR6357088_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	150
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69	32.0	32.0	32.0	32.0	32.0
2	31.56625	32.0	32.0	32.0	32.0	32.0
3	35.67375	37.0	37.0	37.0	32.0	37.0
4	36.19	37.0	37.0	37.0	37.0	37.0
5	36.37125	37.0	37.0	37.0	37.0	37.0
6	39.9215	41.0	41.0	41.0	37.0	41.0
7	40.03175	41.0	41.0	41.0	37.0	41.0
8	40.04925	41.0	41.0	41.0	37.0	41.0
9	40.058	41.0	41.0	41.0	37.0	41.0
10-14	40.111450000000005	41.0	41.0	41.0	41.0	41.0
15-19	39.99135	41.0	41.0	41.0	38.6	41.0
20-24	39.86889999999999	41.0	41.0	41.0	37.0	41.0
25-29	39.852000000000004	41.0	41.0	41.0	37.0	41.0
30-34	39.7329	41.0	41.0	41.0	37.0	41.0
35-39	39.60215	41.0	41.0	41.0	37.0	41.0
40-44	39.52565	41.0	41.0	41.0	37.0	41.0
45-49	39.3664	41.0	41.0	41.0	37.0	41.0
50-54	39.19125	41.0	41.0	41.0	37.0	41.0
55-59	39.102199999999996	41.0	41.0	41.0	37.0	41.0
60-64	38.910000000000004	41.0	41.0	41.0	35.0	41.0
65-69	38.720400000000005	41.0	41.0	41.0	32.0	41.0
70-74	38.6389	41.0	41.0	41.0	32.0	41.0
75-79	38.1723	41.0	39.4	41.0	32.0	41.0
80-84	38.462650000000004	41.0	41.0	41.0	32.0	41.0
85-89	38.293400000000005	41.0	41.0	41.0	32.0	41.0
90-94	38.06	41.0	41.0	41.0	32.0	41.0
95-99	37.855	41.0	41.0	41.0	29.0	41.0
100-104	37.5841	41.0	37.8	41.0	27.0	41.0
105-109	37.24145	41.0	37.0	41.0	24.0	41.0
110-114	36.873400000000004	41.0	37.0	41.0	22.0	41.0
115-119	36.6925	41.0	37.0	41.0	22.0	41.0
120-124	36.43385	41.0	37.0	41.0	22.0	41.0
125-129	36.05445	41.0	37.0	41.0	22.0	41.0
130-134	35.77255	41.0	37.0	41.0	20.0	41.0
135-139	35.490449999999996	41.0	36.0	41.0	12.0	41.0
140-144	34.976	41.0	32.0	41.0	12.0	41.0
145-149	34.70185	41.0	32.0	41.0	12.0	41.0
150	34.5325	41.0	32.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	0.0
16	4.0
17	12.0
18	8.0
19	7.0
20	18.0
21	7.0
22	7.0
23	15.0
24	17.0
25	18.0
26	43.0
27	35.0
28	46.0
29	52.0
30	70.0
31	77.0
32	73.0
33	74.0
34	78.0
35	111.0
36	147.0
37	166.0
38	315.0
39	724.0
40	1873.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.25	10.274999999999999	6.775	39.7
2	32.15803950987747	17.029257314328582	31.98299574893723	18.829707426856714
3	34.7586896724181	21.330332583145786	17.35433858464616	26.556639159789945
4	31.557889472368096	33.633408352088026	13.15328832208052	21.655413853463365
5	32.324999999999996	32.525	16.075	19.075
6	26.03150787696924	34.28357089272318	17.029257314328582	22.655663915978995
7	29.325000000000003	16.775000000000002	30.65	23.25
8	24.5311327831958	18.72968242060515	27.656914228557138	29.08227056764191
9	28.075	19.1	24.55	28.275
10-14	30.458045804580458	22.937293729372936	21.507150715071507	25.0975097509751
15-19	29.969495424313646	22.263339500925138	21.613241986297947	26.153923088463273
20-24	29.769465419812974	22.58338750812622	22.40836125418813	25.23878581787268
25-29	29.239385907886184	25.84887733159974	19.287893183977598	25.62384357653648
30-34	28.447111777944485	24.06101525381345	20.130032508127034	27.361840460115026
35-39	29.370874174834967	24.20984196839368	19.78395679135827	26.635327065413083
40-44	28.412841284128415	22.052205220522055	21.93219321932193	27.602760276027606
45-49	28.748624587376213	23.211963589076724	21.38641592477743	26.652995898769632
50-54	28.057014253563388	21.770442610652662	22.635658914728683	27.536884221055264
55-59	27.9183755126538	22.566770031009302	22.106631989596877	27.408222466740025
60-64	28.512128032008	22.680670167541887	22.875718929732432	25.93148287071768
65-69	27.40459160706247	21.897664182463863	21.307457610163556	29.390286600310105
70-74	27.62690672668167	21.780445111277817	21.47536884221055	29.117279319829958
75-79	28.258477543262977	22.4667400220066	21.796538961688505	27.478243473041914
80-84	27.234085112766916	22.443366504975746	22.463369505425813	27.859178876831525
85-89	28.792956830573758	21.694762643189435	22.32504627082187	27.18723425541494
90-94	27.55964587605662	22.482869004151453	22.032711449007152	27.924773670784774
95-99	28.206282513005203	22.914165666266506	21.388555422168867	27.490996398559425
100-104	27.941985496374095	23.465866466616657	21.470367591897972	27.121780445111277
105-109	28.710742148429684	21.399279855971194	21.579315863172635	28.310662132426483
110-114	28.444955734507076	22.1877657180013	21.707597659180713	27.659680888310913
115-119	28.70430564584688	23.19347902185328	21.328199229884483	26.77401610241536
120-124	28.715743148629723	23.06961392278456	21.794358871774357	26.42028405681136
125-129	27.811952988247064	23.56089022255564	21.790447611902973	26.83670917729432
130-134	29.607401850462615	22.67066766691673	22.710677669417354	25.0112528132033
135-139	28.11624649859944	24.674869947979193	20.9733893557423	26.23549419767907
140-144	29.017254313578395	22.91072768192048	23.05076269067267	25.021255313828455
145-149	28.822205551387846	24.621155288822205	21.015253813453363	25.541385346336583
150	26.91345672836418	24.58729364682341	20.735367683841922	27.763881940970485
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	1.0
29	1.0
30	0.5
31	1.0
32	1.0
33	2.0
34	2.5
35	3.5
36	6.0
37	13.0
38	12.5
39	12.0
40	15.0
41	13.5
42	13.5
43	11.0
44	10.0
45	8.5
46	13.0
47	20.0
48	34.5
49	75.5
50	156.0
51	236.0
52	242.0
53	219.5
54	210.0
55	185.0
56	231.0
57	400.5
58	495.0
59	453.5
60	331.0
61	197.0
62	133.5
63	88.0
64	47.5
65	23.0
66	11.0
67	6.0
68	5.5
69	4.5
70	4.5
71	7.0
72	5.0
73	2.5
74	5.0
75	4.5
76	2.5
77	3.0
78	4.0
79	3.0
80	1.5
81	1.5
82	2.0
83	2.0
84	1.5
85	0.5
86	1.0
87	1.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.025
3	0.025
4	0.025
5	0.0
6	0.025
7	0.0
8	0.025
9	0.0
10-14	0.01
15-19	0.015
20-24	0.015
25-29	0.015
30-34	0.025
35-39	0.02
40-44	0.01
45-49	0.03
50-54	0.025
55-59	0.03
60-64	0.025
65-69	0.034999999999999996
70-74	0.025
75-79	0.03
80-84	0.015
85-89	0.045
90-94	0.034999999999999996
95-99	0.04
100-104	0.025
105-109	0.02
110-114	0.034999999999999996
115-119	0.015
120-124	0.02
125-129	0.025
130-134	0.025
135-139	0.04
140-144	0.025
145-149	0.025
150	0.05
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.12500000000001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.20430107526882	44.875
2	10.064516129032258	11.700000000000001
3	4.21505376344086	7.35
4	2.193548387096774	5.1
5	2.1505376344086025	6.25
6	0.8602150537634409	3.0
7	0.8602150537634409	3.5000000000000004
8	0.5591397849462365	2.6
9	0.4731182795698925	2.475
>10	1.3763440860215055	11.774999999999999
>50	0.043010752688172046	1.375
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGG	55	1.375	No Hit
GTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCT	29	0.7250000000000001	No Hit
GTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACC	27	0.675	No Hit
GTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCC	26	0.65	No Hit
GGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACA	21	0.525	No Hit
GTCAACTTCAAGTTCCTGAGCCACTGCCTGCTGGTGACCTTGGCTTGCCA	19	0.475	No Hit
CTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTG	17	0.42500000000000004	No Hit
TGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGAC	16	0.4	No Hit
CTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTT	16	0.4	No Hit
GTCCACTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCCTGTCA	15	0.375	No Hit
GGAAAAGAGGTTTAGTGGTACTTGTGAGCCAGGGCACTGGCCACTCCAGC	15	0.375	No Hit
CCAAGACCTACTTCTCTCACATTGATGTAAGCCCCGGCTCTGCCCAGGTC	14	0.35000000000000003	No Hit
GACAAAACCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATGGTGG	14	0.35000000000000003	No Hit
CCCGGCTCTGCCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTT	14	0.35000000000000003	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	14	0.35000000000000003	Illumina Single End PCR Primer 1 (100% over 50bp)
GTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCA	13	0.325	No Hit
CGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCTGCAGACCACGTCGAAG	13	0.325	No Hit
GCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCC	13	0.325	No Hit
CTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCCTGTCAACTTC	13	0.325	No Hit
CGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCT	13	0.325	No Hit
GGGGAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTACAGA	13	0.325	No Hit
GGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGG	13	0.325	No Hit
GGAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCT	13	0.325	No Hit
CCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATGGTGGTGAATAT	12	0.3	No Hit
GGAATTTGTCCAGAGAGGCGTGCATGGCGGGTGTGAAATCTCCAGGGTGG	12	0.3	No Hit
CGGGTGTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAG	12	0.3	No Hit
GCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACAGGATC	11	0.27499999999999997	No Hit
GGTGAATATGGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCAC	11	0.27499999999999997	No Hit
CAGGAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAA	11	0.27499999999999997	No Hit
AGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGTC	11	0.27499999999999997	No Hit
GGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACGG	10	0.25	No Hit
CGAAGACCTGCCTGGTGCCCTGTCCACTCTGAGCGACCTGCATGCCCACA	10	0.25	No Hit
AGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATT	10	0.25	No Hit
GGAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACT	9	0.22499999999999998	No Hit
CACAAACTCAGAAACAGACACCATGGTGCACCTGACTGATGCTGAGAAGG	9	0.22499999999999998	No Hit
CTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGG	9	0.22499999999999998	No Hit
CTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTTCTTGCCG	9	0.22499999999999998	No Hit
GGGAAAGGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGC	9	0.22499999999999998	No Hit
GTTGTGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTGACTG	9	0.22499999999999998	No Hit
GGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCAACCTT	9	0.22499999999999998	No Hit
GTGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGA	9	0.22499999999999998	No Hit
GCCCTGTCCACTCTGAGCGACCTGCATGCCCACAAACTGCGTGTGGATCC	9	0.22499999999999998	No Hit
CCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGC	9	0.22499999999999998	No Hit
CTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAA	9	0.22499999999999998	No Hit
GTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCAT	8	0.2	No Hit
CAGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGT	8	0.2	No Hit
GCGGGTGTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCA	8	0.2	No Hit
CCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCT	8	0.2	No Hit
CTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCACTGTGACAAGCTGCA	8	0.2	No Hit
GAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCC	8	0.2	No Hit
GGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGGGCATGCAGGTCG	8	0.2	No Hit
GCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTC	8	0.2	No Hit
GCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTG	8	0.2	No Hit
GGGACCTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCC	8	0.2	No Hit
CTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCA	8	0.2	No Hit
GTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGA	8	0.2	No Hit
GTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTGT	8	0.2	No Hit
GGCGGCTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGA	7	0.17500000000000002	No Hit
CGGCAAGAAGGTTGCTGATGCCCTGGCCAAAGCTGCAGACCACGTCGAAG	7	0.17500000000000002	No Hit
CTGCAGATGACAAAACCAACATCAAGAACTGCTGGGGGAAGATTGGTGGC	7	0.17500000000000002	No Hit
CTGAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACT	7	0.17500000000000002	No Hit
CTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCT	7	0.17500000000000002	No Hit
GAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACGGT	7	0.17500000000000002	No Hit
AAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACTGC	7	0.17500000000000002	No Hit
GGGGCTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGGGGAAGGCAG	7	0.17500000000000002	No Hit
TGGGCATGCAGGTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGAC	7	0.17500000000000002	No Hit
CTTGGCCAAAGCTGCAGACCACGTCGAAGACCTGCCTGGTGCCCTGTCCA	7	0.17500000000000002	No Hit
CTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGGGGAAGGCAGCGAA	7	0.17500000000000002	No Hit
CAAACTGCGTGTGGATCCTGTCAACTTCAAGTTCCTGAGCCACTGCCTGC	7	0.17500000000000002	No Hit
CTCCACTGTGACAAGCTGCATGTGGATCCTGAGAACTTCAGGCTCCTGGG	7	0.17500000000000002	No Hit
CCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCTGCTATC	7	0.17500000000000002	No Hit
CTCGGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCAT	7	0.17500000000000002	No Hit
GGATGTTCGCTGCCTTCCCCACCACCAAGACCTACTTCTCTCACATTGAT	7	0.17500000000000002	No Hit
CTGATGCCTTGGCCAAAGCTGCAGACCACGTCGAAGACCTGCCTGGTGCC	7	0.17500000000000002	No Hit
CTGGTGCCCTGTCCACTCTGAGCGACCTGCATGCCCACAAACTGCGTGTG	7	0.17500000000000002	No Hit
GCCTGAAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAA	7	0.17500000000000002	No Hit
CAGACACCATGGTGCACCTGACTGATGCTGAGAAGGCTGCTGTTAATGGC	7	0.17500000000000002	No Hit
GGCTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGGGGAAGGCAGCG	6	0.15	No Hit
GTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGG	6	0.15	No Hit
GGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCA	6	0.15	No Hit
GTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATGGCA	6	0.15	No Hit
AAACACTTGGACAACCTCAAGGGCACCTTTGCTCATCTGAGTGAACTCCA	6	0.15	No Hit
GAGAACTTCAGGCTCCTGGGCAATATGATTGTGATTGTGTTGGGCCACCA	6	0.15	No Hit
CGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCT	6	0.15	No Hit
CACAAACTGCGTGTGGATCCTGTCAACTTCAAGTTCCTGAGCCACTGCCT	6	0.15	No Hit
CTTGGGAACAATTGACCATTGCACAAAGACAAGAGCAGGAAAAGAGGTTT	6	0.15	No Hit
GTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGTTGACAGGATCCACACG	6	0.15	No Hit
CTGGTTGTCTACCCTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCT	6	0.15	No Hit
GGCTGCTGTTAATGGCCTGTGGGGAAAGGTGAACCCTGATGATGTTGGTG	6	0.15	No Hit
GTCGCTCAGAGTGGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAG	6	0.15	No Hit
CCGGCTCTGCCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTTG	6	0.15	No Hit
GTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTAT	6	0.15	No Hit
CACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCATCAGCA	6	0.15	No Hit
GGGTAACCCTAAGGTGAAGGCCCATGGCAAGAAGGTGATAAACGCCTTCA	6	0.15	No Hit
CCTTGGACCCAGAGGTACTTTGATAGCTTTGGGGACCTGTCCTCTGCCTC	6	0.15	No Hit
GAAGCAATCATGGTGCTCTCTGCAGATGACAAAACCAACATCAAGAACTG	6	0.15	No Hit
GTGCTCTCTGCAGATGACAAAACCAACATCAAGAACTGCTGGGGGAAGAT	6	0.15	No Hit
CGCCTTCAATGATGGCCTGAAACACTTGGACAACCTCAAGGGCACCTTTG	5	0.125	No Hit
GGTGAACCCTGATGATGTTGGTGGCGAGGCCCTGGGCAGGCTGCTGGTTG	5	0.125	No Hit
CTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGT	5	0.125	No Hit
CTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATGGCAAGAAGGTGATA	5	0.125	No Hit
CTGTCCTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATGG	5	0.125	No Hit
CTGGGGGAAGATTGGTGGCCATGGTGGTGAATATGGCGAGGAGGCCCTAC	5	0.125	No Hit
CCTTGACCTGGGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGTAGGTC	5	0.125	No Hit
GGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTTAACG	5	0.125	No Hit
CTGGGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGTAGGTCTTGGTGG	5	0.125	No Hit
CTTCTCTCACATTGATGTAAGCCCCGGCTCTGCCCAGGTCAAGGCTCACG	5	0.125	No Hit
GGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGG	5	0.125	No Hit
CTTGACCTGGGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGTAGGTCT	5	0.125	No Hit
CAGGCAGTGGCTCAGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGT	5	0.125	No Hit
AGATGACAAAACCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATG	5	0.125	No Hit
CTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAATTTG	5	0.125	No Hit
CTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAAC	5	0.125	No Hit
CCGGGGCTTACATCAATGTGAGAGAAGTAGGTCTTGGTGGTGGGGAAGGC	5	0.125	No Hit
GGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGC	5	0.125	No Hit
CGTGCATGGCGGGTGTGAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACC	5	0.125	No Hit
CCTGCATGCCCACAAACTGCGTGTGGATCCTGTCAACTTCAAGTTCCTGA	5	0.125	No Hit
CGGGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCT	5	0.125	No Hit
GTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTTGGCCAAAGCTGCAGA	5	0.125	No Hit
GGACAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAGGG	5	0.125	No Hit
CGGCTCTGCCCAGGTCAAGGCTCACGGCAAGAAGGTTGCTGATGCCTTGG	5	0.125	No Hit
CCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTT	5	0.125	No Hit
AAGGGAGGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGC	5	0.125	No Hit
GTGAGCCTTGACCTGGGCAGAGCCGGGGCTTACATCAATGTGAGAGAAGT	5	0.125	No Hit
GTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTT	5	0.125	No Hit
CGGCTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAAT	5	0.125	No Hit
GCTTAACGGTACTTGGAGGTCAGCACAGTGCTCACAGAGGCAAGGAATTT	5	0.125	No Hit
GAAATCTCCAGGGTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCA	5	0.125	No Hit
CAAAACCAACATCAAGAACTGCTGGGGGAAGATTGGTGGCCATGGTGGTG	5	0.125	No Hit
GGCGAGGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGAC	5	0.125	No Hit
CTTTGATAGCTTTGGGGACCTGTCCTCTGCCTCTGCTATCATGGGTAACC	5	0.125	No Hit
CTCACAGAGGCAAGGAATTTGTCCAGAGAGGCGTGCATGGCGGGTGTGAA	5	0.125	No Hit
GTGCAAGGGAGGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGG	5	0.125	No Hit
GAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCA	5	0.125	No Hit
GTGGTGGCAAGCCAAGGTCACCAGCAGGCAGTGGCTCAGGAACTTGAAGT	5	0.125	No Hit
GTCAGCACAGTGCTCACAGAGGCAAGGAATTTGTCCAGAGAGGCGTGCAT	5	0.125	No Hit
GGCAGTGGCTCAGGAACTTGAAGTTGACAGGATCCACACGCAGTTTGTGG	5	0.125	No Hit
GGCGAGGCCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGAGGTA	5	0.125	No Hit
GGAGGCCCTACAGAGGATGTTCGCTGCCTTCCCCACCACCAAGACCTACT	5	0.125	No Hit
GGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGACAGGAGGTGGCTTAACGG	5	0.125	No Hit
CATAGTTGTGTTGACTCACAAACTCAGAAACAGACACCATGGTGCACCTG	5	0.125	No Hit
CTCTGCCTCTGCTATCATGGGTAACCCTAAGGTGAAGGCCCATGGCAAGA	5	0.125	No Hit
CTTAACGGTACTTGGAGGTCAGCACGGTGCTCACAGAGGCAAGGAATTTG	5	0.125	No Hit
CTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCT	5	0.125	No Hit
GGGAGGGAAGAAGGGCCTGGTCAGAAGGCAAGCCCGGCAGGAGGCGGCTT	5	0.125	No Hit
CCCTGGGCAGGCTGCTGGTTGTCTACCCTTGGACCCAGAGGTACTTTGAT	5	0.125	No Hit
CAGGGCACCAGGCAGGTCTTCGACGTGGTCTGCAGCTTTGGCCAAGGCAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0125	0.0	0.0	0.0	0.0
28-29	0.025	0.0	0.0	0.0	0.0
30-31	0.037500000000000006	0.0	0.0	0.0	0.0
32-33	0.05	0.0	0.0	0.0	0.0
34-35	0.05	0.0	0.0	0.0	0.0
36-37	0.0625	0.0	0.0	0.0	0.0
38-39	0.125	0.0	0.0	0.0	0.0
40-41	0.16249999999999998	0.0	0.0	0.0	0.0
42-43	0.2	0.0	0.0	0.0	0.0
44-45	0.25	0.0	0.0	0.0	0.0
46-47	0.2875	0.0	0.0	0.0	0.0
48-49	0.325	0.0	0.0	0.0	0.0
50-51	0.3375	0.0	0.0	0.0	0.0
52-53	0.4	0.0	0.0	0.0	0.0
54-55	0.45	0.0	0.0	0.0	0.0
56-57	0.475	0.0	0.0	0.0	0.0
58-59	0.475	0.0	0.0	0.0	0.0
60-61	0.5125	0.0	0.0	0.0	0.0
62-63	0.575	0.0	0.0	0.0	0.0
64-65	0.675	0.0	0.0	0.0	0.0
66-67	0.7625	0.0	0.0	0.0	0.0
68-69	0.825	0.0	0.0	0.0	0.0
70-71	0.85	0.0	0.0	0.0	0.0
72-73	0.8875	0.0	0.0	0.0	0.0
74-75	0.975	0.0	0.0	0.0	0.0
76-77	1.1125	0.0	0.0	0.0	0.0
78-79	1.2374999999999998	0.0	0.0	0.0	0.0
80-81	1.3375	0.0	0.0	0.0	0.0
82-83	1.3875	0.0	0.0	0.0	0.0
84-85	1.475	0.0	0.0	0.0	0.0
86-87	1.625	0.0	0.0	0.0	0.0
88-89	1.775	0.0	0.0	0.0	0.0
90-91	1.875	0.0	0.0	0.0	0.0
92-93	2.0625	0.0	0.0	0.0	0.0
94-95	2.2625	0.0	0.0	0.0	0.0
96-97	2.4625000000000004	0.0	0.0	0.0	0.0
98-99	2.6125	0.0	0.0	0.0	0.0
100-101	2.9625000000000004	0.0	0.0	0.0	0.0
102-103	3.2750000000000004	0.0	0.0	0.0	0.0
104-105	3.575	0.0	0.0	0.0	0.0
106-107	3.9125	0.0	0.0	0.0	0.0
108-109	4.324999999999999	0.0	0.0	0.0	0.0
110-111	4.8125	0.0	0.0	0.0	0.0
112-113	5.3125	0.0	0.0	0.0	0.0
114-115	5.775	0.0	0.0	0.0	0.0
116-117	6.25	0.0	0.0	0.0	0.0
118-119	6.5625	0.0	0.0	0.0	0.0
120-121	7.1875	0.0	0.0	0.0	0.0
122-123	7.75	0.0	0.0	0.0	0.0
124-125	8.3	0.0	0.0	0.0	0.0
126-127	9.0125	0.0	0.0	0.0	0.0
128-129	9.6125	0.0	0.0	0.0	0.0
130-131	10.087499999999999	0.0	0.0	0.0	0.0
132-133	10.7125	0.0	0.0	0.0	0.0
134-135	11.425	0.0	0.0	0.0	0.0
136-137	12.4	0.0	0.0	0.0	0.0
138	13.125	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGGTG	30	0.0018473949	72.0	6
GATTGGT	30	0.0018473949	72.0	5
GGAAGAT	30	0.0018473949	72.0	1
AGATTGG	30	0.0018473949	72.0	4
GAAGATT	35	0.0034045284	61.714283	2
AAGATTG	35	0.0034045284	61.714283	3
>>END_MODULE
Read 3591009 spots for ERR6357088.sra
Written 3591009 spots for ERR6357088.sra
Read 3591009 spots for ERR6357088.sra
Written 3591009 spots for ERR6357088.sra
Read 3591009 spots for ERR6357088.sra
Written 3591009 spots for ERR6357088.sra
Read 3591015 spots for ERR6357088.sra
Written 3591015 spots for ERR6357088.sra
Read 3591009 spots for ERR6357088.sra
Written 3591009 spots for ERR6357088.sra
Read 3591009 spots for ERR6357088.sra
Written 3591009 spots for ERR6357088.sra
Read 3591009 spots for ERR6357088.sra
Written 3591009 spots for ERR6357088.sra
Read 3591009 spots for ERR6357088.sra
Written 3591009 spots for ERR6357088.sra
SRR ids: ['ERR6357088.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_ofuynvue
ERR6357088.sra spots: 28728078
blocks: [[1, 3591009], [3591010, 7182018], [7182019, 10773027], [10773028, 14364036], [14364037, 17955045], [17955046, 21546054], [21546055, 25137063], [25137064, 28728078]]
ERR6357088 file size 9657204
ERR6357088 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o ERR6357088 ERR6357088_1.fastq ERR6357088_2.fastq
Input file:	ERR6357088_1.fastq
Paired file:	ERR6357088_2.fastq
trimmed:	ERR6357088-trimmed-pair1.fastq, ERR6357088-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Mon Dec 29 01:33:53 2025 >> started

Mon Dec 29 01:34:36 2025 >> done (42.118s)
28728078 read pairs processed; of these:
    2470 ( 0.01%) short read pairs filtered out after trimming by size control
   96109 ( 0.33%) empty read pairs filtered out after trimming by size control
28629499 (99.66%) read pairs available; of these:
 6074421 (21.22%) trimmed read pairs available after processing
22555078 (78.78%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     311	  0.00%
 19	     356	  0.00%
 20	     372	  0.00%
 21	     476	  0.00%
 22	     500	  0.00%
 23	     612	  0.00%
 24	     637	  0.00%
 25	     688	  0.00%
 26	     775	  0.00%
 27	     958	  0.00%
 28	    1071	  0.00%
 29	    1036	  0.00%
 30	    1360	  0.00%
 31	    1327	  0.00%
 32	    1392	  0.00%
 33	    1655	  0.01%
 34	    1486	  0.01%
 35	    1743	  0.01%
 36	    1984	  0.01%
 37	    1914	  0.01%
 38	    2355	  0.01%
 39	    2118	  0.01%
 40	    2448	  0.01%
 41	    2969	  0.01%
 42	    2927	  0.01%
 43	    3233	  0.01%
 44	    3129	  0.01%
 45	    3063	  0.01%
 46	    3667	  0.01%
 47	    3652	  0.01%
 48	    3905	  0.01%
 49	    4527	  0.02%
 50	    4597	  0.02%
 51	    5050	  0.02%
 52	    5140	  0.02%
 53	    5276	  0.02%
 54	    5274	  0.02%
 55	    4810	  0.02%
 56	    5300	  0.02%
 57	    5999	  0.02%
 58	    5680	  0.02%
 59	    6457	  0.02%
 60	    6038	  0.02%
 61	    7129	  0.02%
 62	    7225	  0.03%
 63	    7253	  0.03%
 64	    7518	  0.03%
 65	    7557	  0.03%
 66	    8732	  0.03%
 67	    8402	  0.03%
 68	   10207	  0.04%
 69	    9091	  0.03%
 70	   10049	  0.04%
 71	   10134	  0.04%
 72	   10758	  0.04%
 73	   11787	  0.04%
 74	   11195	  0.04%
 75	   11139	  0.04%
 76	   11113	  0.04%
 77	   11136	  0.04%
 78	   12974	  0.05%
 79	   13024	  0.05%
 80	   13044	  0.05%
 81	   14091	  0.05%
 82	   16523	  0.06%
 83	   16644	  0.06%
 84	   17028	  0.06%
 85	   17158	  0.06%
 86	   18075	  0.06%
 87	   19136	  0.07%
 88	   19935	  0.07%
 89	   21798	  0.08%
 90	   21717	  0.08%
 91	   22895	  0.08%
 92	   23757	  0.08%
 93	   27313	  0.10%
 94	   30318	  0.11%
 95	   31861	  0.11%
 96	   31695	  0.11%
 97	   28336	  0.10%
 98	   31034	  0.11%
 99	   31485	  0.11%
100	   32987	  0.12%
101	   32346	  0.11%
102	   35834	  0.13%
103	   43788	  0.15%
104	   44963	  0.16%
105	   50432	  0.18%
106	   42298	  0.15%
107	   47554	  0.17%
108	   46927	  0.16%
109	   55126	  0.19%
110	   55064	  0.19%
111	   55075	  0.19%
112	   52574	  0.18%
113	   57194	  0.20%
114	   68407	  0.24%
115	   69451	  0.24%
116	   64257	  0.22%
117	   70321	  0.25%
118	   65544	  0.23%
119	   68896	  0.24%
120	   64261	  0.22%
121	   73405	  0.26%
122	   71822	  0.25%
123	   79804	  0.28%
124	   86041	  0.30%
125	   86864	  0.30%
126	   89736	  0.31%
127	   81575	  0.28%
128	  103640	  0.36%
129	   82324	  0.29%
130	   95272	  0.33%
131	   99024	  0.35%
132	  108913	  0.38%
133	  100720	  0.35%
134	  106546	  0.37%
135	  127160	  0.44%
136	  125089	  0.44%
137	  125075	  0.44%
138	  126693	  0.44%
139	  121538	  0.42%
140	  119654	  0.42%
141	  127329	  0.44%
142	  138897	  0.49%
143	  124380	  0.43%
144	  153720	  0.54%
145	  143481	  0.50%
146	  172327	  0.60%
147	  188260	  0.66%
148	  249635	  0.87%
149	  883665	  3.09%
150	22555078	 78.78%
28629499 reads passed initial QC


criterion=sequence-density
sequence-density=4.87
sequence-density-rank=1
fanout-score=2.39
fanout-score-rank=40
prefix-density=4.79
prefix-fanout=2.4
sequence=GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA


criterion=fanout-score
sequence-density=0.54
sequence-density-rank=20
fanout-score=35.79
fanout-score-rank=1
prefix-density=9.97
prefix-fanout=2.0
sequence=GCAAGGAATTCTCCCCCTGTGCA


criterion=sequence-density
sequence-density=4.69
sequence-density-rank=1
fanout-score=2.40
fanout-score-rank=40
prefix-density=4.64
prefix-fanout=2.4
sequence=GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA


criterion=fanout-score
sequence-density=0.56
sequence-density-rank=19
fanout-score=34.35
fanout-score-rank=1
prefix-density=9.71
prefix-fanout=2.0
sequence=GCAAGGAATTCTCCCCCTGTGCA
Potential 3prime adapter identified. Now checking if in reference sequence
/dee2/code/volunteer_pipeline.sh: line 989: -f: command not found
/dee2/code/volunteer_pipeline.sh: line 990: -f: command not found
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA -y GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA -o ERR6357088 ERR6357088_1.fastq ERR6357088_2.fastq
Input file:	ERR6357088_1.fastq
Paired file:	ERR6357088_2.fastq
trimmed:	ERR6357088-trimmed-pair1.fastq, ERR6357088-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGA
-- paired 3' end adapter sequence (-y):	GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Mon Dec 29 01:36:02 2025 >> started

Mon Dec 29 01:36:30 2025 >> done (28.053s)
17177700 read pairs processed; of these:
   11035 ( 0.06%) short read pairs filtered out after trimming by size control
  146477 ( 0.85%) empty read pairs filtered out after trimming by size control
17020188 (99.08%) read pairs available; of these:
    2630 ( 0.02%) trimmed read pairs available after processing
17017558 (99.98%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	     196	  0.00%
 19	     201	  0.00%
 20	     222	  0.00%
 21	     279	  0.00%
 22	     301	  0.00%
 23	     386	  0.00%
 24	     367	  0.00%
 25	     425	  0.00%
 26	     470	  0.00%
 27	     568	  0.00%
 28	     627	  0.00%
 29	     617	  0.00%
 30	     810	  0.00%
 31	     774	  0.00%
 32	     823	  0.00%
 33	     983	  0.01%
 34	     922	  0.01%
 35	    1031	  0.01%
 36	    1220	  0.01%
 37	    1142	  0.01%
 38	    1378	  0.01%
 39	    1252	  0.01%
 40	    1438	  0.01%
 41	    1775	  0.01%
 42	    1768	  0.01%
 43	    1923	  0.01%
 44	    1900	  0.01%
 45	    1840	  0.01%
 46	    2216	  0.01%
 47	    2191	  0.01%
 48	    2322	  0.01%
 49	    2711	  0.02%
 50	    2739	  0.02%
 51	    3012	  0.02%
 52	    3004	  0.02%
 53	    3048	  0.02%
 54	    3071	  0.02%
 55	    2775	  0.02%
 56	    3121	  0.02%
 57	    3514	  0.02%
 58	    3387	  0.02%
 59	    3843	  0.02%
 60	    3596	  0.02%
 61	    4299	  0.03%
 62	    4271	  0.03%
 63	    4317	  0.03%
 64	    4482	  0.03%
 65	    4536	  0.03%
 66	    5195	  0.03%
 67	    5060	  0.03%
 68	    6049	  0.04%
 69	    5413	  0.03%
 70	    6026	  0.04%
 71	    5993	  0.04%
 72	    6372	  0.04%
 73	    7031	  0.04%
 74	    6646	  0.04%
 75	    6658	  0.04%
 76	    6632	  0.04%
 77	    6677	  0.04%
 78	    7734	  0.05%
 79	    7889	  0.05%
 80	    7755	  0.05%
 81	    8267	  0.05%
 82	    9936	  0.06%
 83	    9913	  0.06%
 84	   10199	  0.06%
 85	   10354	  0.06%
 86	   10830	  0.06%
 87	   11437	  0.07%
 88	   11961	  0.07%
 89	   12983	  0.08%
 90	   12841	  0.08%
 91	   13632	  0.08%
 92	   14048	  0.08%
 93	   16320	  0.10%
 94	   18106	  0.11%
 95	   19108	  0.11%
 96	   19013	  0.11%
 97	   16865	  0.10%
 98	   18515	  0.11%
 99	   18733	  0.11%
100	   19786	  0.12%
101	   19217	  0.11%
102	   21381	  0.13%
103	   26333	  0.15%
104	   27055	  0.16%
105	   29990	  0.18%
106	   25342	  0.15%
107	   28543	  0.17%
108	   28107	  0.17%
109	   33056	  0.19%
110	   33098	  0.19%
111	   33013	  0.19%
112	   31575	  0.19%
113	   34240	  0.20%
114	   40860	  0.24%
115	   41469	  0.24%
116	   38250	  0.22%
117	   41921	  0.25%
118	   38864	  0.23%
119	   40976	  0.24%
120	   38176	  0.22%
121	   43741	  0.26%
122	   43052	  0.25%
123	   47556	  0.28%
124	   51223	  0.30%
125	   51690	  0.30%
126	   53327	  0.31%
127	   48830	  0.29%
128	   62168	  0.37%
129	   48678	  0.29%
130	   56158	  0.33%
131	   58411	  0.34%
132	   65431	  0.38%
133	   60022	  0.35%
134	   63925	  0.38%
135	   75911	  0.45%
136	   74341	  0.44%
137	   73783	  0.43%
138	   74736	  0.44%
139	   71455	  0.42%
140	   70666	  0.42%
141	   74703	  0.44%
142	   82672	  0.49%
143	   73614	  0.43%
144	   91579	  0.54%
145	   84579	  0.50%
146	  101652	  0.60%
147	  110746	  0.65%
148	  147547	  0.87%
149	  524312	  3.08%
150	13410144	 78.79%


criterion=sequence-density
sequence-density=4.91
sequence-density-rank=1
fanout-score=2.40
fanout-score-rank=40
prefix-density=4.95
prefix-fanout=2.4
sequence=GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA


criterion=fanout-score
sequence-density=0.56
sequence-density-rank=19
fanout-score=33.67
fanout-score-rank=1
prefix-density=9.66
prefix-fanout=2.0
sequence=GCAAGGAATTCTCCCCCTGTGCA


criterion=sequence-density
sequence-density=4.78
sequence-density-rank=1
fanout-score=2.41
fanout-score-rank=40
prefix-density=4.82
prefix-fanout=2.4
sequence=GGGGTGAATTCCTTGCCCAGGTGGTGGCCCAACACAATCACAATCATATTGCCCAGGAGCCTGAAGTTCTCAGGATCCACATGCAGCTTGTCACAGTGGAGTTCACTCAGATGAGCAAAGGTGCCCTTGAGGTTGTCCAAGTGTTTCAGGCCATCATTGAAGGCGTTTATCACCTTCTTGCCATGGGCCTTCACCTTAGGGTTACCCATGATAGCAGAGGCAGAGGACAGGTCCCCAAAGCTATCAAAGTACCTCTGGGTCCAAGGGTAGACAACCAGCAGCCTGCCCAGGGCCTCGCCACCAACATCATCAGGGTTCACCTTTCCCCACAGGCCATTAACAGCAGCCTTCTCAGCATCAGTCAGGTGCACCATGGTGTCTGTTTCTGAGTTTGTGAGTCAACACAACTATGTCAGAAGCAAA


criterion=fanout-score
sequence-density=0.56
sequence-density-rank=18
fanout-score=33.23
fanout-score-rank=1
prefix-density=9.35
prefix-fanout=2.0
sequence=GCAAGGAATTCTCCCCCTGTGCA
ERR6357088 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Dec 29 01:37:51
                             Started mapping on |	Dec 29 01:37:51
                                    Finished on |	Dec 29 01:50:12
       Mapping speed, Million of reads per hour |	138.33

                          Number of input reads |	28471987
                      Average input read length |	290
                                    UNIQUE READS:
                   Uniquely mapped reads number |	21916310
                        Uniquely mapped reads % |	76.97%
                          Average mapped length |	288.73
                       Number of splices: Total |	28055216
            Number of splices: Annotated (sjdb) |	27500914
                       Number of splices: GT/AG |	27926528
                       Number of splices: GC/AG |	28451
                       Number of splices: AT/AC |	2129
               Number of splices: Non-canonical |	98108
                      Mismatch rate per base, % |	0.51%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.97
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.75
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	5686457
             % of reads mapped to multiple loci |	19.97%
        Number of reads mapped to too many loci |	66987
             % of reads mapped to too many loci |	0.24%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	2.80%
                     % of reads unmapped: other |	0.02%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	869220	869220	869220
N_multimapping	5686457	5686457	5686457
N_noFeature	392142	11048973	11163393
N_ambiguous	403708	149875	158099
UnstrandedReadsAssigned:21120460 PositiveStrandReadsAssigned:10717462 NegativeStrandReadsAssigned:10594818
Dataset is classified unstranded
MeadianReadLen=150 20thPercentileLength=150 echo kmer=145
ERR6357088 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 31,715
[index] number of k-mers: 49,915,185
[index] number of equivalence classes: 70,351
[quant] running in paired-end mode
[quant] will process pair 1: ERR6357088-trimmed-pair1.fastq
                             ERR6357088-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 28,471,987 reads, 23,201,899 reads pseudoaligned
[quant] estimated average fragment length: 195.563
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 983 rounds

  31716 ERR6357088.ke.tsv
  32883 ERR6357088.se.tsv
  64599 total
==> ERR6357088.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	760.482	2522	61.0397
ENSRNOT00000040993.4	1039	844.437	1939	42.2637
ENSRNOT00000050156.3	1545	1350.44	19870	270.82
ENSRNOT00000043693.3	684	489.525	5575	209.617
ENSRNOT00000046201.3	204	49.6	167	61.9714
ENSRNOT00000046108.3	681	486.525	6170	233.419
ENSRNOT00000049683.3	784	589.525	9991	311.935
ENSRNOT00000041241.3	348	156.076	364	42.9263
ENSRNOT00000044582.3	297	110.365	130	21.6806

==> ERR6357088.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	0
ENSRNOG00000058808	0
ENSRNOG00000061316	6
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	0
ERR6357088 completed mapping pipeline successfully
