Starting /dee2/code/volunteer_pipeline.sh SRR14127855
    current disk space = 640867280
    free memory = 197314612 
SRR14127855 SRAfilesize
70e2182d329abff44fff68adbcf92b6e  SRR14127855.sra
SRR14127855.sra file validated
SRR14127855 is single end
SRR14127855 is conventional basespace
SRR14127855 read1 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR14127855_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.34	37.0	37.0	37.0	37.0	37.0
2	35.819	37.0	37.0	37.0	37.0	37.0
3	36.2465	37.0	37.0	37.0	37.0	37.0
4	36.4605	37.0	37.0	37.0	37.0	37.0
5	36.492	37.0	37.0	37.0	37.0	37.0
6	36.4415	37.0	37.0	37.0	37.0	37.0
7	36.5495	37.0	37.0	37.0	37.0	37.0
8	36.6195	37.0	37.0	37.0	37.0	37.0
9	36.639	37.0	37.0	37.0	37.0	37.0
10	36.5655	37.0	37.0	37.0	37.0	37.0
11	36.4925	37.0	37.0	37.0	37.0	37.0
12	36.6185	37.0	37.0	37.0	37.0	37.0
13	36.503	37.0	37.0	37.0	37.0	37.0
14	36.421	37.0	37.0	37.0	37.0	37.0
15	36.5015	37.0	37.0	37.0	37.0	37.0
16	36.2595	37.0	37.0	37.0	37.0	37.0
17	36.397	37.0	37.0	37.0	37.0	37.0
18	36.3055	37.0	37.0	37.0	37.0	37.0
19	36.283	37.0	37.0	37.0	37.0	37.0
20	36.448	37.0	37.0	37.0	37.0	37.0
21	36.368	37.0	37.0	37.0	37.0	37.0
22	36.5785	37.0	37.0	37.0	37.0	37.0
23	36.4925	37.0	37.0	37.0	37.0	37.0
24	36.404	37.0	37.0	37.0	37.0	37.0
25	36.5945	37.0	37.0	37.0	37.0	37.0
26	36.4855	37.0	37.0	37.0	37.0	37.0
27	36.523	37.0	37.0	37.0	37.0	37.0
28	36.402	37.0	37.0	37.0	37.0	37.0
29	36.498	37.0	37.0	37.0	37.0	37.0
30	36.6475	37.0	37.0	37.0	37.0	37.0
31	36.57	37.0	37.0	37.0	37.0	37.0
32	36.516	37.0	37.0	37.0	37.0	37.0
33	36.631	37.0	37.0	37.0	37.0	37.0
34	36.3965	37.0	37.0	37.0	37.0	37.0
35	36.543	37.0	37.0	37.0	37.0	37.0
36	36.6075	37.0	37.0	37.0	37.0	37.0
37	36.5925	37.0	37.0	37.0	37.0	37.0
38	36.668	37.0	37.0	37.0	37.0	37.0
39	36.5385	37.0	37.0	37.0	37.0	37.0
40	36.446	37.0	37.0	37.0	37.0	37.0
41	36.382	37.0	37.0	37.0	37.0	37.0
42	36.49375	37.0	37.0	37.0	37.0	37.0
43	36.3895	37.0	37.0	37.0	37.0	37.0
44	36.4565	37.0	37.0	37.0	37.0	37.0
45	36.676	37.0	37.0	37.0	37.0	37.0
46	36.773	37.0	37.0	37.0	37.0	37.0
47	36.559	37.0	37.0	37.0	37.0	37.0
48	36.589	37.0	37.0	37.0	37.0	37.0
49	36.565	37.0	37.0	37.0	37.0	37.0
50	36.4625	37.0	37.0	37.0	37.0	37.0
51	36.638	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	3.0
26	3.0
27	6.0
28	9.0
29	20.0
30	31.0
31	29.0
32	43.0
33	69.0
34	91.0
35	151.0
36	1123.0
37	2421.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	15.075	7.675	71.72500000000001	5.525
2	21.457286432160807	29.773869346733665	10.402010050251256	38.36683417085427
3	21.375	55.300000000000004	8.375	14.95
4	41.3	12.1	19.2	27.400000000000002
5	15.950000000000001	24.725	50.324999999999996	9.0
6	44.324999999999996	8.450000000000001	33.275	13.950000000000001
7	24.075	25.650000000000002	17.474999999999998	32.800000000000004
8	22.325	39.800000000000004	32.2	5.675
9	19.0	15.275	21.9	43.824999999999996
10	27.1	26.025	30.9	15.975
11	27.950000000000003	29.099999999999998	25.424999999999997	17.525
12	16.25	26.950000000000003	23.125	33.675
13	13.825000000000001	33.5	15.475	37.2
14	34.575	20.674999999999997	38.324999999999996	6.425
15	46.225	42.449999999999996	6.8500000000000005	4.475
16	7.049999999999999	54.85	30.975	7.124999999999999
17	17.825	18.2	30.875000000000004	33.1
18	45.625	10.475	35.525	8.375
19	16.45	19.725	54.949999999999996	8.875
20	21.4	18.975	55.60000000000001	4.025
21	60.175	14.549999999999999	14.249999999999998	11.025
22	14.799999999999999	40.949999999999996	35.575	8.674999999999999
23	16.075	61.25000000000001	8.200000000000001	14.475
24	50.724999999999994	26.825	18.05	4.3999999999999995
25	17.150000000000002	56.45	19.375	7.025
26	15.15	19.0	44.175	21.675
27	15.25	15.375	22.400000000000002	46.975
28	56.325	8.475000000000001	10.424999999999999	24.775
29	66.25	17.299999999999997	3.2750000000000004	13.175
30	26.825	54.725	3.025	15.425
31	22.7	60.650000000000006	4.375	12.275
32	48.85	34.300000000000004	8.924999999999999	7.925
33	26.875	59.975	5.8500000000000005	7.3
34	58.050000000000004	21.4	5.050000000000001	15.5
35	17.125	27.375	6.875000000000001	48.625
36	15.174999999999999	51.575	8.175	25.074999999999996
37	8.200000000000001	26.450000000000003	5.175	60.175
38	12.275	56.425000000000004	5.4	25.900000000000002
39	17.299999999999997	19.3	6.675000000000001	56.725
40	48.575	20.1	11.85	19.475
41	18.825	12.125	45.975	23.075000000000003
42	19.529882470617654	11.327831957989497	21.705426356589147	47.436859214803704
43	16.6	13.05	52.925	17.424999999999997
44	50.075	18.3	14.325	17.299999999999997
45	16.35	57.8	11.725	14.124999999999998
46	14.424999999999999	62.075	4.75	18.75
47	5.975	27.575	11.200000000000001	55.25
48	6.8500000000000005	18.6	43.875	30.675
49	7.7	8.625	14.475	69.19999999999999
50	10.05	14.725	14.099999999999998	61.12499999999999
51	13.350000000000001	46.400000000000006	10.6	29.65
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.5
26	0.5
27	0.0
28	0.0
29	0.0
30	0.5
31	1.0
32	0.5
33	0.0
34	1.0
35	2.0
36	4.0
37	6.0
38	7.0
39	8.0
40	15.5
41	23.0
42	82.0
43	141.0
44	373.5
45	606.0
46	984.5
47	1363.0
48	984.0
49	605.0
50	567.0
51	529.0
52	415.0
53	301.0
54	228.0
55	155.0
56	132.0
57	109.0
58	103.0
59	97.0
60	69.0
61	41.0
62	24.5
63	8.0
64	5.0
65	2.0
66	2.0
67	2.0
68	1.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.5
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.025
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.45
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.26964560862865	24.425
2	11.40215716486903	7.3999999999999995
3	3.5439137134052388	3.45
4	1.848998459167951	2.4
5	1.4637904468412943	2.375
6	0.7704160246533128	1.5
7	0.847457627118644	1.925
8	0.46224961479198773	1.2
9	0.3852080123266564	1.125
>10	3.389830508474576	21.675
>50	0.30816640986132515	7.55
>100	0.23112480739599386	8.7
>500	0.07704160246533129	16.275000000000002
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCCA	651	16.275000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCCA	122	3.05	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT	117	2.9250000000000003	No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTCC	109	2.725	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACGTCTGAACTCCA	92	2.3	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACTC	85	2.125	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA	66	1.6500000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCCA	59	1.4749999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC	42	1.05	No Hit
TAATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACTC	42	1.05	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAACACTGTCTGGTAACGATGTAGATCGGAAGAGCACACGTCTGAACTCCA	39	0.975	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCCA	38	0.95	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG	38	0.95	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAACACTGTCTGGTAACGATGTTAGATCGGAAGAGCACACGTCTGAACTCC	38	0.95	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACTC	33	0.8250000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCAG	32	0.8	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCCA	26	0.65	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC	26	0.65	No Hit
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG	25	0.625	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCCA	25	0.625	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACAC	22	0.5499999999999999	No Hit
TGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT	22	0.5499999999999999	TruSeq Adapter, Index 11 (100% over 37bp)
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCAG	20	0.5	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCCA	19	0.475	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAATACTGCCTGGTAATGATGACAAGATCGGAAGAGCACACGTCTGAACTC	19	0.475	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC	19	0.475	TruSeq Adapter, Index 6 (97% over 36bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTCC	19	0.475	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGTGCACTACAGAACTTTGAAGATCGGAAGAGCACACGTCTGAACTCCA	17	0.42500000000000004	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAATACTGCCGGGTAATGATGGAAGATCGGAAGAGCACACGTCTGAACTCC	16	0.4	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGTGCACTACAGAACTTTGGAGATCGGAAGAGCACACGTCTGAACTCCA	16	0.4	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAACACTGTCTGGTAACGATGAGATCGGAAGAGCACACGTCTGAACTCCAG	15	0.375	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCCA	15	0.375	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACCAGATCGGAAGAGCACACGTCTGAACTC	15	0.375	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAATACTGCCTGGTAATGATGAGATCGGAAGAGCACACGTCTGAACTCCAG	15	0.375	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGGTTGTATGGTTAGATCGGAAGAGCACACGTCTGAACTCCA	15	0.375	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACGT	14	0.35000000000000003	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT	14	0.35000000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT	14	0.35000000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAATACTGCCTGGTAATGATGAAGATCGGAAGAGCACACGTCTGAACTCCA	13	0.325	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AATACTGCCTGGTAATGATGACTAGATCGGAAGAGCACACGTCTGAACTCC	12	0.3	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCCA	12	0.3	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAATACTGCCTGGTAATGATGACCAGATCGGAAGAGCACACGTCTGAACTC	12	0.3	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACACG	12	0.3	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAAGATCGGAAGAGCACACGT	12	0.3	No Hit
TGAGATGAAGCACTGTAGCTCCAGATCGGAAGAGCACACGTCTGAACTCCA	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAATACTGCCTGGTAATGATGACAGATCGGAAGAGCACACGTCTGAACTCC	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACGAGATCGGAAGAGCACACGTCTGAACTC	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACACG	11	0.27499999999999997	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACACG	10	0.25	No Hit
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCCA	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACTC	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC	10	0.25	No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAGATCGGAAGAGCAC	9	0.22499999999999998	No Hit
TCAGTGCATGACAGAACTTGGAGATCGGAAGAGCACACGTCTGAACTCCAG	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAACACTGTCTGGTAACGATGTTTAGATCGGAAGAGCACACGTCTGAACTC	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGTTTGTGCTGTCAGATCGGAAGAGCACACGTCTGAACTCCA	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTC	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGCTTATCAGACTGATGTTGAAAGATCGGAAGAGCACACGTCTGAACTCC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCCA	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTCAGATCGGAAGAGCACACGTCTGAACTCCA	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTAAACATCCTACACTCAGCTAGATCGGAAGAGCACACGTCTGAACTCCA	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG	7	0.17500000000000002	No Hit
TGAGATGAAGCACTGTAGCTCGAGATCGGAAGAGCACACGTCTGAACTCCA	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTTAGATCGGAAGAGCACACGTCTGAACTCC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGATCGGAAGAGCACAC	7	0.17500000000000002	No Hit
TGAGGTAGTAGTTTGTACAGTTTAGATCGGAAGAGCACACGTCTGAACTCC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCACTGATGAAACTAGAAATAGGAAGTGCCGTCCGAAACGACAACTGACG	7	0.17500000000000002	No Hit
TGAGGTAGTAGATTGTATAGTTTAGATCGGAAGAGCACACGTCTGAACTCC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTAGATCGGAAGAGCACA	7	0.17500000000000002	No Hit
TGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACA	7	0.17500000000000002	TruSeq Adapter, Index 11 (100% over 40bp)
TCAGTGCATGACAGAACTTGGTAGATCGGAAGAGCACACGTCTGAACTCCA	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTCC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAATACTGCCGGGTAATGATGGATAGATCGGAAGAGCACACGTCTGAACTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAAAGACACCATACTGAATAGAAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCAAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGATTGTATAGCTAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCACA	6	0.15	No Hit
TAGCTTATCAGACTGATGTTAAGATCGGAAGAGCACACGTCTGAACTCCAG	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCAGTGCATCACAGAACTTTGTTAGATCGGAAGAGCACACGTCTGAACTCC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATTCAGGATAGGTTAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCCTGTAGATCCGAATTTGTGAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TNAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGATGAAGCACTGTAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTCC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAAGACTAGTGATTTTGTTGTTAGATCGGAAGAGCACACGTCTGAACTC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGTAAACATCCTCGACTGGAAAAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTAGATCGGAAGAGCACA	5	0.125	No Hit
CTGGACGCGAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT	5	0.125	TruSeq Adapter, Index 11 (100% over 37bp)
TAATACTGCCTGGTAATGATGACGAGATCGGAAGAGCACACGTCTGAACTC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCACCATCTGAAATCGGTTAAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAG	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTAGATCGGAAGAGCACA	5	0.125	No Hit
GGTGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG	5	0.125	TruSeq Adapter, Index 2 (97% over 35bp)
CATCTTACCGGACAGTGCTGGAAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCACCATCTGAAATCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAG	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCTTGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAATACTGCCGGGTAATGATGGAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.1	0.0	0.0	0.0	0.0
2	0.1	0.0	0.0	0.0	0.0
3	0.1	0.0	0.0	0.0	0.0
4	0.1	0.0	0.0	0.0	0.0
5	0.1	0.0	0.0	0.0	0.0
6	0.125	0.0	0.0	0.0	0.0
7	0.2	0.0	0.0	0.0	0.0
8	0.3	0.0	0.0	0.0	0.0
9	0.475	0.0	0.0	0.0	0.0
10	0.725	0.0	0.0	0.0	0.0
11	1.35	0.0	0.0	0.0	0.0
12	2.375	0.0	0.0	0.0	0.0
13	3.425	0.0	0.0	0.0	0.0
14	5.175	0.0	0.0	0.0	0.0
15	6.425	0.0	0.0	0.0	0.0
16	7.4	0.0	0.0	0.0	0.0
17	8.2	0.0	0.0	0.0	0.0
18	9.325	0.0	0.0	0.0	0.0
19	10.325	0.0	0.0	0.0	0.0
20	11.1	0.0	0.0	0.0	0.0
21	13.1	0.0	0.0	0.0	0.0
22	21.3	0.0	0.0	0.0	0.0
23	62.325	0.0	0.0	0.0	0.0
24	72.625	0.0	0.0	0.0	0.0
25	81.125	0.0	0.0	0.0	0.0
26	81.75	0.0	0.0	0.0	0.0
27	82.175	0.0	0.0	0.0	0.0
28	82.675	0.0	0.0	0.0	0.0
29	83.15	0.0	0.0	0.0	0.0
30	83.525	0.0	0.0	0.0	0.0
31	84.55	0.0	0.0	0.0	0.0
32	87.25	0.0	0.0	0.0	0.0
33	91.55	0.0	0.0	0.0	0.0
34	93.15	0.0	0.0	0.0	0.0
35	94.675	0.0	0.0	0.0	0.0
36	95.725	0.0	0.0	0.0	0.0
37	96.425	0.0	0.0	0.0	0.0
38	96.625	0.0	0.0	0.0	0.0
39	96.95	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACAGA	130	0.0	45.000004	9
TCAGTGC	135	0.0	45.000004	1
TGCACTA	140	0.0	45.0	5
GAGGTAG	20	6.222983E-4	45.0	2
GTAGAAT	35	8.569259E-8	45.0	18
ACTGTAG	20	6.222983E-4	45.0	12
TGGTTCA	35	8.569259E-8	45.0	8
CATTGGT	35	8.569259E-8	45.0	2
GTGCACT	140	0.0	45.0	4
AGCTTAT	20	6.222983E-4	45.0	2
ACTGATG	20	6.222983E-4	45.0	12
AATTCTC	35	8.569259E-8	45.0	22
TGGTAGA	35	8.569259E-8	45.0	16
CGCCTAG	20	6.222983E-4	45.0	28
GCCTAGA	20	6.222983E-4	45.0	29
GATGTTG	20	6.222983E-4	45.0	15
ATTGGTG	35	8.569259E-8	45.0	3
AGTGGTA	35	8.569259E-8	45.0	14
GAAGCAC	20	6.222983E-4	45.0	7
TTATCAG	20	6.222983E-4	45.0	5
>>END_MODULE
Rejected 2144535 READS because READLEN < 1
Read 2144535 spots for SRR14127855.sra
Written 2144535 spots for SRR14127855.sra
Rejected 2144535 READS because READLEN < 1
Read 2144535 spots for SRR14127855.sra
Written 2144535 spots for SRR14127855.sra
Rejected 2144535 READS because READLEN < 1
Read 2144535 spots for SRR14127855.sra
Written 2144535 spots for SRR14127855.sra
Rejected 2144535 READS because READLEN < 1
Read 2144535 spots for SRR14127855.sra
Written 2144535 spots for SRR14127855.sra
Rejected 2144535 READS because READLEN < 1
Read 2144535 spots for SRR14127855.sra
Written 2144535 spots for SRR14127855.sra
Rejected 2144535 READS because READLEN < 1
Read 2144535 spots for SRR14127855.sra
Written 2144535 spots for SRR14127855.sra
Rejected 2144535 READS because READLEN < 1
Read 2144535 spots for SRR14127855.sra
Written 2144535 spots for SRR14127855.sra
Rejected 2144535 READS because READLEN < 1
Read 2144535 spots for SRR14127855.sra
Written 2144535 spots for SRR14127855.sra
SRR ids: ['SRR14127855.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_8997jxm8
SRR14127855.sra spots: 17156280
blocks: [[1, 2144535], [2144536, 4289070], [4289071, 6433605], [6433606, 8578140], [8578141, 10722675], [10722676, 12867210], [12867211, 15011745], [15011746, 17156280]]
SRR14127855 file size 2441164
SRR14127855 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR14127855 SRR14127855_1.fastq
Input file:	SRR14127855_1.fastq
trimmed:	SRR14127855-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Tue Dec  2 20:18:06 2025 >> started

Tue Dec  2 20:18:13 2025 >> done (6.746s)
17156280 reads processed; of these:
 1652287 ( 9.63%) short reads filtered out after trimming by size control
   28933 ( 0.17%) empty reads filtered out after trimming by size control
15475060 (90.20%) reads available; of these:
  326514 ( 2.11%) trimmed reads available after processing
15148546 (97.89%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  148053	  0.96%
 19	  178461	  1.15%
 20	       0	  0.00%
 21	       0	  0.00%
 22	       0	  0.00%
 23	       0	  0.00%
 24	       0	  0.00%
 25	       0	  0.00%
 26	       0	  0.00%
 27	       0	  0.00%
 28	       0	  0.00%
 29	       0	  0.00%
 30	       0	  0.00%
 31	       0	  0.00%
 32	       0	  0.00%
 33	       0	  0.00%
 34	       0	  0.00%
 35	       0	  0.00%
 36	       0	  0.00%
 37	       0	  0.00%
 38	       0	  0.00%
 39	       0	  0.00%
 40	       0	  0.00%
 41	       0	  0.00%
 42	       0	  0.00%
 43	       0	  0.00%
 44	       0	  0.00%
 45	       0	  0.00%
 46	       0	  0.00%
 47	       0	  0.00%
 48	       0	  0.00%
 49	       0	  0.00%
 50	       0	  0.00%
 51	15148546	 97.89%
15475060 reads passed initial QC


criterion=sequence-density
sequence-density=95.04
sequence-density-rank=1
fanout-score=11.09
fanout-score-rank=1
prefix-density=95.79
prefix-fanout=11.0
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCT


criterion=fanout-score
sequence-density=95.04
sequence-density-rank=1
fanout-score=11.09
fanout-score-rank=1
prefix-density=95.79
prefix-fanout=11.0
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCT -o SRR14127855 -
Input file:	STDIN
trimmed:	SRR14127855-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Tue Dec  2 20:18:26 2025 >> started

Tue Dec  2 20:18:39 2025 >> done (12.448s)
15152663 reads processed; of these:
    5956 ( 0.04%) short reads filtered out after trimming by size control
      98 ( 0.00%) empty reads filtered out after trimming by size control
15146609 (99.96%) reads available; of these:
14723258 (97.20%) trimmed reads available after processing
  423351 ( 2.80%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  143353	  0.95%
 19	  176832	  1.17%
 20	  402089	  2.65%
 21	 1276865	  8.43%
 22	 6995967	 46.19%
 23	 1819086	 12.01%
 24	 1421406	  9.38%
 25	  107488	  0.71%
 26	   70410	  0.46%
 27	   72078	  0.48%
 28	   92854	  0.61%
 29	   54741	  0.36%
 30	  151937	  1.00%
 31	  535826	  3.54%
 32	  728692	  4.81%
 33	  256607	  1.69%
 34	  224072	  1.48%
 35	  189769	  1.25%
 36	  125209	  0.83%
 37	   41398	  0.27%
 38	   38328	  0.25%
 39	   26874	  0.18%
 40	   12195	  0.08%
 41	    7914	  0.05%
 42	    6886	  0.05%
 43	    9426	  0.06%
 44	   20596	  0.14%
 45	   23577	  0.16%
 46	    2203	  0.01%
 47	    1035	  0.01%
 48	    2906	  0.02%
 49	       0	  0.00%
 50	       0	  0.00%
 51	  107990	  0.71%


criterion=sequence-density
sequence-density=23.90
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=7
prefix-density=0.00
prefix-fanout=1.0
sequence=TCAGTGCACTACAGAACTTTGT


criterion=fanout-score
sequence-density=0.29
sequence-density-rank=22
fanout-score=15.92
fanout-score-rank=1
prefix-density=3.90
prefix-fanout=1.2
sequence=CTGTCTGGTAATGCCGTT
                                 Started job on |	Dec 02 20:18:51
                             Started mapping on |	Dec 02 20:18:51
                                    Finished on |	Dec 02 20:19:49
       Mapping speed, Million of reads per hour |	960.15

                          Number of input reads |	15469006
                      Average input read length |	24
                                    UNIQUE READS:
                   Uniquely mapped reads number |	10755321
                        Uniquely mapped reads % |	69.53%
                          Average mapped length |	22.39
                       Number of splices: Total |	12656
            Number of splices: Annotated (sjdb) |	5730
                       Number of splices: GT/AG |	12370
                       Number of splices: GC/AG |	139
                       Number of splices: AT/AC |	10
               Number of splices: Non-canonical |	137
                      Mismatch rate per base, % |	0.12%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.34
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.59
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	2689443
             % of reads mapped to multiple loci |	17.39%
        Number of reads mapped to too many loci |	1647632
             % of reads mapped to too many loci |	10.65%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	2.34%
                     % of reads unmapped: other |	0.09%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	2024242	2024242	2024242
N_multimapping	2689443	2689443	2689443
N_noFeature	347866	1237775	9851931
N_ambiguous	85604	72089	40
UnstrandedReadsAssigned:10321851 PositiveStrandReadsAssigned:9445457 NegativeStrandReadsAssigned:903350
Dataset is classified positive stranded
MeadianReadLen=22 20thPercentileLength=22 echo kmer=19
SRR14127855 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 31,715
[index] number of k-mers: 49,362,984
[index] number of equivalence classes: 166,181
[quant] running in single-end mode
[quant] will process file 1: SRR14127855-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 15,469,006 reads, 241,783 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 742 rounds

  31716 SRR14127855.ke.tsv
  32883 SRR14127855.se.tsv
  64599 total
==> SRR14127855.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	856	1705	6146.87
ENSRNOT00000040993.4	1039	940	2307	7573.96
ENSRNOT00000050156.3	1545	1446	7065	15078.1
ENSRNOT00000043693.3	684	585	2547	13436.2
ENSRNOT00000046201.3	204	105	364	10698.3
ENSRNOT00000046108.3	681	582	1639	8690.79
ENSRNOT00000049683.3	784	685	2423	10916.1
ENSRNOT00000041241.3	348	249	257	3185.2
ENSRNOT00000044582.3	297	198	240	3740.67

==> SRR14127855.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	0
ENSRNOG00000058808	1
ENSRNOG00000061316	0
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	0
SRR14127855 completed mapping pipeline successfully
