Starting /dee2/code/volunteer_pipeline.sh SRR14127854
    current disk space = 1787416616
    free memory = 120372712 
SRR14127854 SRAfilesize
a856b849e659209878c624af5ca8d54e  SRR14127854.sra
SRR14127854.sra file validated
SRR14127854 is single end
SRR14127854 is conventional basespace
SRR14127854 read1 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR14127854_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.788	37.0	37.0	37.0	25.0	37.0
2	35.66925	37.0	37.0	37.0	37.0	37.0
3	36.0325	37.0	37.0	37.0	37.0	37.0
4	36.365	37.0	37.0	37.0	37.0	37.0
5	36.4315	37.0	37.0	37.0	37.0	37.0
6	36.317	37.0	37.0	37.0	37.0	37.0
7	36.3825	37.0	37.0	37.0	37.0	37.0
8	36.568	37.0	37.0	37.0	37.0	37.0
9	36.459	37.0	37.0	37.0	37.0	37.0
10	36.4345	37.0	37.0	37.0	37.0	37.0
11	36.437	37.0	37.0	37.0	37.0	37.0
12	36.5895	37.0	37.0	37.0	37.0	37.0
13	36.5655	37.0	37.0	37.0	37.0	37.0
14	36.586	37.0	37.0	37.0	37.0	37.0
15	36.478	37.0	37.0	37.0	37.0	37.0
16	36.4065	37.0	37.0	37.0	37.0	37.0
17	36.463	37.0	37.0	37.0	37.0	37.0
18	36.438	37.0	37.0	37.0	37.0	37.0
19	36.56	37.0	37.0	37.0	37.0	37.0
20	36.477	37.0	37.0	37.0	37.0	37.0
21	36.3765	37.0	37.0	37.0	37.0	37.0
22	36.612	37.0	37.0	37.0	37.0	37.0
23	36.519	37.0	37.0	37.0	37.0	37.0
24	36.414	37.0	37.0	37.0	37.0	37.0
25	36.573	37.0	37.0	37.0	37.0	37.0
26	36.4305	37.0	37.0	37.0	37.0	37.0
27	36.4425	37.0	37.0	37.0	37.0	37.0
28	36.4325	37.0	37.0	37.0	37.0	37.0
29	36.4075	37.0	37.0	37.0	37.0	37.0
30	36.6935	37.0	37.0	37.0	37.0	37.0
31	36.5605	37.0	37.0	37.0	37.0	37.0
32	36.4705	37.0	37.0	37.0	37.0	37.0
33	36.645	37.0	37.0	37.0	37.0	37.0
34	36.479	37.0	37.0	37.0	37.0	37.0
35	36.552	37.0	37.0	37.0	37.0	37.0
36	36.6255	37.0	37.0	37.0	37.0	37.0
37	36.5905	37.0	37.0	37.0	37.0	37.0
38	36.689	37.0	37.0	37.0	37.0	37.0
39	36.584	37.0	37.0	37.0	37.0	37.0
40	36.36	37.0	37.0	37.0	37.0	37.0
41	36.4545	37.0	37.0	37.0	37.0	37.0
42	36.554	37.0	37.0	37.0	37.0	37.0
43	36.2365	37.0	37.0	37.0	37.0	37.0
44	36.4	37.0	37.0	37.0	37.0	37.0
45	36.69	37.0	37.0	37.0	37.0	37.0
46	36.675	37.0	37.0	37.0	37.0	37.0
47	36.573	37.0	37.0	37.0	37.0	37.0
48	36.506	37.0	37.0	37.0	37.0	37.0
49	36.4765	37.0	37.0	37.0	37.0	37.0
50	36.473	37.0	37.0	37.0	37.0	37.0
51	36.5655	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	2.0
26	4.0
27	5.0
28	12.0
29	23.0
30	37.0
31	33.0
32	42.0
33	56.0
34	113.0
35	164.0
36	1289.0
37	2220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.275	7.925	63.375	3.4250000000000003
2	49.63613550815558	12.8732747804266	10.238393977415306	27.25219573400251
3	14.499999999999998	66.425	6.275	12.8
4	48.475	14.725	23.775	13.025
5	13.525	52.075	27.975	6.425
6	32.4	9.675	52.65	5.2749999999999995
7	66.0	14.05	11.325000000000001	8.625
8	11.75	48.6	36.325	3.325
9	27.85	48.3	15.425	8.425
10	65.375	16.25	11.924999999999999	6.45
11	16.825000000000003	8.825	27.450000000000003	46.9
12	8.674999999999999	47.3	30.45	13.575000000000001
13	5.1499999999999995	15.625	11.425	67.80000000000001
14	12.675	32.225	50.64999999999999	4.45
15	80.175	11.3	4.425	4.1000000000000005
16	5.925	18.575	70.25	5.25
17	27.500000000000004	47.925000000000004	17.025000000000002	7.55
18	67.77499999999999	10.9	12.525	8.799999999999999
19	15.325	7.825	37.175000000000004	39.675
20	20.674999999999997	28.000000000000004	46.400000000000006	4.925
21	33.300000000000004	15.375	13.0	38.324999999999996
22	13.425	46.375	32.1	8.1
23	17.9	67.65	8.625	5.825
24	42.05	25.6	28.375	3.975
25	14.249999999999998	42.625	34.8	8.325000000000001
26	12.125	13.075000000000001	38.975	35.825
27	21.95	7.9	29.775000000000002	40.375
28	55.35	10.674999999999999	2.85	31.125000000000004
29	69.5	23.5	1.675	5.325
30	18.8	56.25	2.325	22.625
31	20.925	57.375	4.1000000000000005	17.599999999999998
32	48.225	37.325	8.325000000000001	6.125
33	35.975	52.325	2.75	8.95
34	46.75	27.6	5.0	20.65
35	12.15	26.825	13.0	48.025
36	8.85	47.15	7.425	36.575
37	18.3	26.474999999999998	3.225	52.0
38	23.025000000000002	45.9	5.075	26.0
39	22.325	24.2	8.674999999999999	44.800000000000004
40	43.824999999999996	20.974999999999998	17.549999999999997	17.65
41	24.875	12.675	43.875	18.575
42	17.575	18.4	23.05	40.975
43	26.775	18.275	40.225	14.725
44	45.625	26.0	10.9	17.474999999999998
45	11.35	64.775	5.575	18.3
46	4.575	57.3	7.225	30.9
47	3.3000000000000003	24.325	15.325	57.05
48	4.8	14.075	39.15	41.975
49	13.925	9.875	13.575000000000001	62.625
50	12.525	17.7	16.75	53.025
51	15.9	41.3	16.475	26.325
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	0.5
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.5
29	1.0
30	0.5
31	0.0
32	0.0
33	0.0
34	1.0
35	2.0
36	3.5
37	5.0
38	7.5
39	10.0
40	25.0
41	40.0
42	68.0
43	96.0
44	183.5
45	271.0
46	397.0
47	523.0
48	903.5
49	1284.0
50	1136.0
51	988.0
52	764.5
53	541.0
54	338.0
55	135.0
56	90.0
57	45.0
58	40.0
59	35.0
60	25.0
61	15.0
62	10.5
63	6.0
64	4.0
65	2.0
66	1.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.375
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.2301918265221	22.85
2	10.925771476230192	6.550000000000001
3	3.419516263552961	3.075
4	1.58465387823186	1.9
5	1.4178482068390326	2.125
6	0.7506255212677231	1.35
7	0.8340283569641368	1.7500000000000002
8	0.5838198498748958	1.4000000000000001
9	0.4170141784820684	1.125
>10	2.9190992493744785	18.2
>50	0.5004170141784821	10.45
>100	0.3336113427856547	15.35
>500	0.08340283569641367	13.875000000000002
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA	555	13.875000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC	207	5.175	No Hit
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG	155	3.875	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT	131	3.2750000000000004	No Hit
TGAGATGAAGCACTGTAGCTCCAGATCGGAAGAGCACACGTCTGAACTCCA	121	3.025	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT	97	2.4250000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCCA	73	1.825	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTTAGATCGGAAGAGCACACGTCTGAACTCC	68	1.7000000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC	67	1.675	No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCCA	61	1.525	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCCA	52	1.3	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTCC	45	1.125	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG	44	1.0999999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCCA	42	1.05	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT	40	1.0	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGAAAGTATGCAAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC	40	1.0	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC	39	0.975	No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCCA	26	0.65	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG	26	0.65	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC	26	0.65	No Hit
TGAGATGAAGCACTGTAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT	25	0.625	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGATGAAGCACTGTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAG	22	0.5499999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACAC	22	0.5499999999999999	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACGT	22	0.5499999999999999	No Hit
TGGAATGTAAAGAAGTATGTATAGATCGGAAGAGCACACGTCTGAACTCCA	22	0.5499999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCCA	21	0.525	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA	19	0.475	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCGAGATCGGAAGAGCACACGTCTGAACTCCA	18	0.44999999999999996	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTAGATCGGAAGAGCACACGTCTGAACTCCA	18	0.44999999999999996	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCAG	17	0.42500000000000004	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCAAGTAATTCAGGATAGGTTAGATCGGAAGAGCACACGTCTGAACTCCA	17	0.42500000000000004	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT	14	0.35000000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTACCGTGAGTAATAATGCAGATCGGAAGAGCACACGTCTGAACTCCAG	14	0.35000000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTAGATCGGAAGAGCACA	13	0.325	No Hit
TTCACAGTGGCTAAGTTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAG	13	0.325	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGATGAAGCACTGTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC	13	0.325	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCCA	13	0.325	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCACA	12	0.3	No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC	12	0.3	No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT	12	0.3	No Hit
TGAGATGAAGCACTGTAGCTCTAAGATCGGAAGAGCACACGTCTGAACTCC	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCGCTAGATCGGAAGAGCACACGTCTGAACTCCA	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCCA	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTCAGATCGGAAGAGCACACGTCTGAACTCC	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTCAGATCGGAAGAGCACACGTCTGAACTCCA	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCAAGATCGGAAGAGCACACGTCTGAACTCCA	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCGAGATCGGAAGAGCACACGT	9	0.22499999999999998	No Hit
AACCCGTAGATCCGATCTTGTGAAGATCGGAAGAGCACACGTCTGAACTCC	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCAGATCGGAAGAGCACACGTCTGAACTCCAG	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACTC	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGAACTGAATTCCATGGGTTAGATCGGAAGAGCACACGTCTGAACTCCA	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGAAAGTATGCAAGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCAAGTAATCCAGGATAGGCCAGATCGGAAGAGCACACGTCTGAACTCCA	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAG	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC	8	0.2	No Hit
GTCCAGTTTTCCCAGGAATCCCTAGATCGGAAGAGCACACGTCTGAACTCC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGAAGATCGGAAGAGCACACGTCT	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGAATGATGATTTCACAGACTAGAGTCTCTGACTCTAGATCGGAAGAGCA	7	0.17500000000000002	No Hit
TTCAAGTAATCCAGGATAGGAGATCGGAAGAGCACACGTCTGAACTCCAGT	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGATGAAGCACTGTAGCTTTAGATCGGAAGAGCACACGTCTGAACTCCA	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTAGATCGGAAGAGCACACGTCTGAACTCC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGAAAGTATGCAAGAACCAGATCGGAAGAGCACACGTCTGAACTCCAGTC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCCA	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACGTCTGAACTCCA	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAAGATCGGAAGAGCACACGT	7	0.17500000000000002	No Hit
TGTAAACATCCTCGACTGGAAAGATCGGAAGAGCACACGTCTGAACTCCAG	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACGT	6	0.15	No Hit
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCAG	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAG	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTAAGATCGGAAGAGCACACGTCTGAACTCC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACACG	6	0.15	No Hit
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTAGATCGGAAGAGCACACGTCTGAACTCCAG	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTCC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTCC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTCT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGTACCGTGAGTAATAATGCGAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTAGATCGGAAGAGCACACGTCTGAACTCC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGATCTTGCAGATCGGAAGAGCACACGTCTGAACTCCAG	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCAG	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGCTTATCAGACTGATGTTGTAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAGGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACGT	5	0.125	No Hit
AAGAAAGTATGCAAGAACTGCTAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCGAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCACCATCTGAAATCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAG	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCTTGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGCTCAGTTCAGCAGGAACAGTAGATCGGAAGAGCACACGTCTGAACTCC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.05	0.0	0.0	0.0	0.0
2	0.075	0.0	0.0	0.0	0.0
3	0.075	0.0	0.0	0.0	0.0
4	0.1	0.0	0.0	0.0	0.0
5	0.1	0.0	0.0	0.0	0.0
6	0.1	0.0	0.0	0.0	0.0
7	0.15	0.0	0.0	0.0	0.0
8	0.175	0.0	0.0	0.0	0.0
9	0.275	0.0	0.0	0.0	0.0
10	0.55	0.0	0.0	0.0	0.0
11	0.725	0.0	0.0	0.0	0.0
12	1.1	0.0	0.0	0.0	0.0
13	1.55	0.0	0.0	0.0	0.0
14	2.375	0.0	0.0	0.0	0.0
15	3.175	0.0	0.0	0.0	0.0
16	3.8	0.0	0.0	0.0	0.0
17	4.175	0.0	0.0	0.0	0.0
18	4.75	0.0	0.0	0.0	0.0
19	5.8	0.0	0.0	0.0	0.0
20	8.175	0.0	0.0	0.0	0.0
21	13.5	0.0	0.0	0.0	0.0
22	26.9	0.0	0.0	0.0	0.0
23	63.675	0.0	0.0	0.0	0.0
24	71.825	0.0	0.0	0.0	0.0
25	73.625	0.0	0.0	0.0	0.0
26	74.075	0.0	0.0	0.0	0.0
27	74.3	0.0	0.0	0.0	0.0
28	74.5	0.0	0.0	0.0	0.0
29	74.9	0.0	0.0	0.0	0.0
30	75.425	0.0	0.0	0.0	0.0
31	78.15	0.0	0.0	0.0	0.0
32	88.475	0.0	0.0	0.0	0.0
33	94.25	0.0	0.0	0.0	0.0
34	95.0	0.0	0.0	0.0	0.0
35	96.275	0.0	0.0	0.0	0.0
36	97.325	0.0	0.0	0.0	0.0
37	97.45	0.0	0.0	0.0	0.0
38	97.75	0.0	0.0	0.0	0.0
39	97.825	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTGGT	65	0.0	45.000004	5
ATGGGTG	20	6.222983E-4	45.0	3
AGCTCCA	20	6.222983E-4	45.0	17
AGCTTAT	20	6.222983E-4	45.0	2
ACTGATG	25	3.236105E-5	45.0	12
GATGTTG	25	3.236105E-5	45.0	15
TTATCAG	25	3.236105E-5	45.0	5
TAGCTCC	20	6.222983E-4	45.0	16
CTGATGT	25	3.236105E-5	45.0	13
ATCAGAC	25	3.236105E-5	45.0	7
TCAGACT	25	3.236105E-5	45.0	8
CTTATCA	25	3.236105E-5	45.0	4
GCATGGG	20	6.222983E-4	45.0	1
TATCAGA	25	3.236105E-5	45.0	6
CAGACTG	25	3.236105E-5	45.0	9
GACTGAT	25	3.236105E-5	45.0	11
ATGTTGA	20	6.222983E-4	45.0	16
GGTGGTT	80	0.0	45.0	6
TGGGTGG	20	6.222983E-4	45.0	4
CATGGGT	20	6.222983E-4	45.0	2
>>END_MODULE
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269483 READS because READLEN < 1
Read 269483 spots for SRR14127854.sra
Written 269483 spots for SRR14127854.sra
Rejected 269542 READS because READLEN < 1
Read 269542 spots for SRR14127854.sra
Written 269542 spots for SRR14127854.sra
SRR ids: ['SRR14127854.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_4b2oqhbm
SRR14127854.sra spots: 17246971
blocks: [[1, 269483], [269484, 538966], [538967, 808449], [808450, 1077932], [1077933, 1347415], [1347416, 1616898], [1616899, 1886381], [1886382, 2155864], [2155865, 2425347], [2425348, 2694830], [2694831, 2964313], [2964314, 3233796], [3233797, 3503279], [3503280, 3772762], [3772763, 4042245], [4042246, 4311728], [4311729, 4581211], [4581212, 4850694], [4850695, 5120177], [5120178, 5389660], [5389661, 5659143], [5659144, 5928626], [5928627, 6198109], [6198110, 6467592], [6467593, 6737075], [6737076, 7006558], [7006559, 7276041], [7276042, 7545524], [7545525, 7815007], [7815008, 8084490], [8084491, 8353973], [8353974, 8623456], [8623457, 8892939], [8892940, 9162422], [9162423, 9431905], [9431906, 9701388], [9701389, 9970871], [9970872, 10240354], [10240355, 10509837], [10509838, 10779320], [10779321, 11048803], [11048804, 11318286], [11318287, 11587769], [11587770, 11857252], [11857253, 12126735], [12126736, 12396218], [12396219, 12665701], [12665702, 12935184], [12935185, 13204667], [13204668, 13474150], [13474151, 13743633], [13743634, 14013116], [14013117, 14282599], [14282600, 14552082], [14552083, 14821565], [14821566, 15091048], [15091049, 15360531], [15360532, 15630014], [15630015, 15899497], [15899498, 16168980], [16168981, 16438463], [16438464, 16707946], [16707947, 16977429], [16977430, 17246971]]
SRR14127854 file size 2454188
SRR14127854 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 64 -o SRR14127854 SRR14127854_1.fastq
Input file:	SRR14127854_1.fastq
trimmed:	SRR14127854-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	32
Mon Jan 10 17:18:38 2022 >> started

Mon Jan 10 17:19:22 2022 >> done (44.760s)
17246971 reads processed; of these:
  883278 ( 5.12%) short reads filtered out after trimming by size control
   13021 ( 0.08%) empty reads filtered out after trimming by size control
16350672 (94.80%) reads available; of these:
  506418 ( 3.10%) trimmed reads available after processing
15844254 (96.90%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  127210	  0.78%
 19	  379208	  2.32%
 20	       0	  0.00%
 21	       0	  0.00%
 22	       0	  0.00%
 23	       0	  0.00%
 24	       0	  0.00%
 25	       0	  0.00%
 26	       0	  0.00%
 27	       0	  0.00%
 28	       0	  0.00%
 29	       0	  0.00%
 30	       0	  0.00%
 31	       0	  0.00%
 32	       0	  0.00%
 33	       0	  0.00%
 34	       0	  0.00%
 35	       0	  0.00%
 36	       0	  0.00%
 37	       0	  0.00%
 38	       0	  0.00%
 39	       0	  0.00%
 40	       0	  0.00%
 41	       0	  0.00%
 42	       0	  0.00%
 43	       0	  0.00%
 44	       0	  0.00%
 45	       0	  0.00%
 46	       0	  0.00%
 47	       0	  0.00%
 48	       0	  0.00%
 49	       0	  0.00%
 50	       0	  0.00%
 51	15844254	 96.90%
16350672 reads passed initial QC


criterion=sequence-density
sequence-density=94.95
sequence-density-rank=1
fanout-score=16.25
fanout-score-rank=3
prefix-density=95.55
prefix-fanout=16.2
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG


criterion=fanout-score
sequence-density=1.17
sequence-density-rank=13
fanout-score=32.56
fanout-score-rank=1
prefix-density=37.22
prefix-fanout=1.0
sequence=AGCACTGTAGCCAGATCGGA
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -t 64 -x AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG -o SRR14127854 -
Input file:	STDIN
trimmed:	SRR14127854-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Unknown format (auto detected)
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	32
Mon Jan 10 17:23:42 2022 >> started

Mon Jan 10 17:24:18 2022 >> done (35.816s)
16006447 reads processed; of these:
    7498 ( 0.05%) short reads filtered out after trimming by size control
      24 ( 0.00%) empty reads filtered out after trimming by size control
15998925 (99.95%) reads available; of these:
15456800 (96.61%) trimmed reads available after processing
  542125 ( 3.39%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  121830	  0.76%
 19	  375139	  2.34%
 20	 1077121	  6.73%
 21	 2308986	 14.43%
 22	 6317870	 39.49%
 23	 1397732	  8.74%
 24	  369774	  2.31%
 25	   61889	  0.39%
 26	   44966	  0.28%
 27	   47599	  0.30%
 28	   59462	  0.37%
 29	   67452	  0.42%
 30	  440283	  2.75%
 31	 1683533	 10.52%
 32	  954035	  5.96%
 33	  124289	  0.78%
 34	  184468	  1.15%
 35	  196661	  1.23%
 36	   21901	  0.14%
 37	   26414	  0.17%
 38	   15561	  0.10%
 39	   10558	  0.07%
 40	    8783	  0.05%
 41	    5887	  0.04%
 42	    4079	  0.03%
 43	    5536	  0.03%
 44	    7629	  0.05%
 45	    3913	  0.02%
 46	     783	  0.00%
 47	     361	  0.00%
 48	    1010	  0.01%
 49	       0	  0.00%
 50	       0	  0.00%
 51	   53421	  0.33%


criterion=sequence-density
sequence-density=16.61
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=11
prefix-density=0.00
prefix-fanout=1.0
sequence=GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGC


criterion=fanout-score
sequence-density=0.41
sequence-density-rank=18
fanout-score=92.07
fanout-score-rank=1
prefix-density=37.27
prefix-fanout=1.0
sequence=AGCACTGTAGCCCTT
                                 Started job on |	Jan 10 17:26:07
                             Started mapping on |	Jan 10 17:26:07
                                    Finished on |	Jan 10 17:27:51
       Mapping speed, Million of reads per hour |	565.72

                          Number of input reads |	16343150
                      Average input read length |	24
                                    UNIQUE READS:
                   Uniquely mapped reads number |	10023350
                        Uniquely mapped reads % |	61.33%
                          Average mapped length |	21.43
                       Number of splices: Total |	10013
            Number of splices: Annotated (sjdb) |	4863
                       Number of splices: GT/AG |	9775
                       Number of splices: GC/AG |	72
                       Number of splices: AT/AC |	7
               Number of splices: Non-canonical |	159
                      Mismatch rate per base, % |	0.10%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.42
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.19
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	3068870
             % of reads mapped to multiple loci |	18.78%
        Number of reads mapped to too many loci |	2852058
             % of reads mapped to too many loci |	17.45%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	2.40%
                     % of reads unmapped: other |	0.04%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	3250930	3250930	3250930
N_multimapping	3068870	3068870	3068870
N_noFeature	252663	684957	9581596
N_ambiguous	171014	161549	5
UnstrandedReadsAssigned:9599673 PositiveStrandReadsAssigned:9176844 NegativeStrandReadsAssigned:441749
Dataset is classified positive stranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR14127854 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 31,715
[index] number of k-mers: 49,362,984
[index] number of equivalence classes: 166,181
[quant] running in single-end mode
[quant] will process file 1: SRR14127854-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 16,343,150 reads, 367,308 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 795 rounds

  31716 SRR14127854.ke.tsv
  32883 SRR14127854.se.tsv
  64599 total
==> SRR14127854.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	856	3663	7207.32
ENSRNOT00000040993.4	1039	940	2953	5291.1
ENSRNOT00000050156.3	1545	1446	17876	20821.5
ENSRNOT00000043693.3	684	585	4602	13249.5
ENSRNOT00000046201.3	204	105	599	9608.33
ENSRNOT00000046108.3	681	582	2985	8638.37
ENSRNOT00000049683.3	784	685	3654	8984.38
ENSRNOT00000041241.3	348	249	480	3246.78
ENSRNOT00000044582.3	297	198	737	6269.21

==> SRR14127854.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	0
ENSRNOG00000058808	1
ENSRNOG00000061316	0
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	0
SRR14127854 completed mapping pipeline successfully
