Starting /dee2/code/volunteer_pipeline.sh SRR14127851
    current disk space = 646594252
    free memory = 200695720 
SRR14127851 SRAfilesize
88719e0496eba46272cfc7519607b62b  SRR14127851.sra
SRR14127851.sra file validated
SRR14127851 is single end
SRR14127851 is conventional basespace
SRR14127851 read1 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR14127851_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.3005	37.0	37.0	37.0	37.0	37.0
2	35.90925	37.0	37.0	37.0	37.0	37.0
3	36.336	37.0	37.0	37.0	37.0	37.0
4	36.36	37.0	37.0	37.0	37.0	37.0
5	36.51	37.0	37.0	37.0	37.0	37.0
6	36.336	37.0	37.0	37.0	37.0	37.0
7	36.3985	37.0	37.0	37.0	37.0	37.0
8	36.69	37.0	37.0	37.0	37.0	37.0
9	36.5485	37.0	37.0	37.0	37.0	37.0
10	36.432	37.0	37.0	37.0	37.0	37.0
11	36.6185	37.0	37.0	37.0	37.0	37.0
12	36.5025	37.0	37.0	37.0	37.0	37.0
13	36.359	37.0	37.0	37.0	37.0	37.0
14	33.2105	37.0	37.0	37.0	25.0	37.0
15	36.3935	37.0	37.0	37.0	37.0	37.0
16	36.345	37.0	37.0	37.0	37.0	37.0
17	36.5455	37.0	37.0	37.0	37.0	37.0
18	36.3555	37.0	37.0	37.0	37.0	37.0
19	36.471	37.0	37.0	37.0	37.0	37.0
20	36.471	37.0	37.0	37.0	37.0	37.0
21	36.4225	37.0	37.0	37.0	37.0	37.0
22	36.578	37.0	37.0	37.0	37.0	37.0
23	36.508	37.0	37.0	37.0	37.0	37.0
24	36.416	37.0	37.0	37.0	37.0	37.0
25	36.6065	37.0	37.0	37.0	37.0	37.0
26	36.481	37.0	37.0	37.0	37.0	37.0
27	36.318	37.0	37.0	37.0	37.0	37.0
28	36.2965	37.0	37.0	37.0	37.0	37.0
29	36.422	37.0	37.0	37.0	37.0	37.0
30	36.6275	37.0	37.0	37.0	37.0	37.0
31	36.556	37.0	37.0	37.0	37.0	37.0
32	36.5085	37.0	37.0	37.0	37.0	37.0
33	36.53	37.0	37.0	37.0	37.0	37.0
34	36.47	37.0	37.0	37.0	37.0	37.0
35	36.6445	37.0	37.0	37.0	37.0	37.0
36	36.649	37.0	37.0	37.0	37.0	37.0
37	36.668	37.0	37.0	37.0	37.0	37.0
38	36.593	37.0	37.0	37.0	37.0	37.0
39	36.512	37.0	37.0	37.0	37.0	37.0
40	36.5225	37.0	37.0	37.0	37.0	37.0
41	36.419	37.0	37.0	37.0	37.0	37.0
42	36.465	37.0	37.0	37.0	37.0	37.0
43	36.3655	37.0	37.0	37.0	37.0	37.0
44	36.372	37.0	37.0	37.0	37.0	37.0
45	36.6385	37.0	37.0	37.0	37.0	37.0
46	36.6835	37.0	37.0	37.0	37.0	37.0
47	36.5685	37.0	37.0	37.0	37.0	37.0
48	36.5335	37.0	37.0	37.0	37.0	37.0
49	36.4905	37.0	37.0	37.0	37.0	37.0
50	36.424	37.0	37.0	37.0	37.0	37.0
51	36.449	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	4.0
26	1.0
27	5.0
28	10.0
29	18.0
30	31.0
31	45.0
32	45.0
33	74.0
34	109.0
35	159.0
36	1494.0
37	2005.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.375	6.5	65.17500000000001	5.949999999999999
2	56.270419703443075	11.058054787635085	9.851721538074894	22.819803970846948
3	14.149999999999999	67.77499999999999	5.7	12.375
4	55.35	10.725	20.775	13.15
5	13.5	56.375	23.575	6.550000000000001
6	26.375	8.725	58.325	6.575
7	69.35	11.375	10.65	8.625
8	12.475	53.525	30.075000000000003	3.925
9	25.900000000000002	53.425	12.0	8.674999999999999
10	70.25	12.2	10.35	7.199999999999999
11	13.600000000000001	7.675	22.725	56.00000000000001
12	8.55	55.625	23.825	12.0
13	6.425	13.05	9.4	71.125
14	11.275	26.875	56.225	5.625
15	78.8	10.025	6.525	4.65
16	9.375	14.875	69.95	5.800000000000001
17	23.724999999999998	55.775	14.274999999999999	6.225
18	73.275	9.625	10.05	7.049999999999999
19	13.725000000000001	9.5	28.499999999999996	48.275
20	16.150000000000002	23.075000000000003	53.175	7.6
21	28.299999999999997	17.424999999999997	12.15	42.125
22	16.125	45.725	28.575	9.575
23	21.325	63.3	9.025	6.35
24	42.35	27.775	24.65	5.225
25	13.8	43.325	31.75	11.125
26	13.950000000000001	12.825000000000001	39.900000000000006	33.324999999999996
27	24.825	8.425	24.224999999999998	42.525
28	58.3	11.65	3.95	26.1
29	63.625	24.825	2.9000000000000004	8.649999999999999
30	19.325	57.175	3.5999999999999996	19.900000000000002
31	22.575	54.474999999999994	5.45	17.5
32	47.349999999999994	33.275	11.425	7.95
33	31.474999999999998	52.825	4.775	10.925
34	50.075	22.575	4.675	22.675
35	12.1	32.35	8.575000000000001	46.975
36	7.925	48.675000000000004	11.924999999999999	31.474999999999998
37	11.1	28.499999999999996	4.875	55.525000000000006
38	22.5	44.25	5.675	27.575
39	27.825	17.0	10.45	44.725
40	42.15	19.75	19.2	18.9
41	18.55	15.55	44.0	21.9
42	22.075	12.425	24.95	40.550000000000004
43	23.5	23.65	39.6	13.25
44	49.275000000000006	28.375	10.4	11.95
45	13.0	60.425	5.775	20.8
46	6.800000000000001	59.12500000000001	7.825	26.25
47	5.2	19.45	17.325	58.025000000000006
48	4.8	17.974999999999998	37.875	39.35
49	9.15	13.825000000000001	10.95	66.07499999999999
50	17.549999999999997	21.0	9.975000000000001	51.475
51	19.3	41.8	18.675	20.225
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	1.0
35	2.0
36	2.5
37	3.0
38	5.0
39	7.0
40	12.0
41	17.0
42	33.0
43	49.0
44	118.5
45	188.0
46	290.5
47	393.0
48	882.0
49	1371.0
50	1247.0
51	1123.0
52	785.0
53	447.0
54	306.0
55	165.0
56	126.5
57	88.0
58	88.0
59	88.0
60	66.5
61	45.0
62	26.0
63	7.0
64	5.0
65	3.0
66	2.5
67	2.0
68	2.0
69	2.0
70	1.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.525
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.25
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.09022556390977	25.3
2	12.406015037593985	8.25
3	3.4586466165413534	3.45
4	2.481203007518797	3.3000000000000003
5	0.6015037593984963	1.0
6	0.6015037593984963	1.2
7	0.6015037593984963	1.4000000000000001
8	0.37593984962406013	1.0
9	0.5263157894736842	1.575
>10	2.330827067669173	17.825
>50	0.07518796992481204	2.4
>100	0.37593984962406013	19.025
>500	0.07518796992481204	14.274999999999999
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA	571	14.274999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG	220	5.5	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT	156	3.9	No Hit
TGAGATGAAGCACTGTAGCTCCAGATCGGAAGAGCACACGTCTGAACTCCA	146	3.65	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCCA	129	3.225	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT	110	2.75	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG	96	2.4	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCGAGATCGGAAGAGCACACGTCTGAACTCCA	41	1.0250000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACGT	39	0.975	No Hit
TGAGATGAAGCACTGTAGCTCTTAGATCGGAAGAGCACACGTCTGAACTCC	39	0.975	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC	39	0.975	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAAGATCGGAAGAGCACACGT	39	0.975	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC	38	0.95	No Hit
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTCC	37	0.9249999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCCA	37	0.9249999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT	36	0.8999999999999999	No Hit
TGAGATGAAGCACTGTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAG	30	0.75	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTCCAGTTTTCCCAGGAATCCCTAGATCGGAAGAGCACACGTCTGAACTCC	29	0.7250000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT	26	0.65	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAGAAAGTATGCAAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGTC	25	0.625	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGATGAAGCACTGTAGCTTTAGATCGGAAGAGCACACGTCTGAACTCCA	24	0.6	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCCA	21	0.525	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCCA	20	0.5	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCACA	18	0.44999999999999996	No Hit
TGAGATGAAGCACTGTAGCGCTAGATCGGAAGAGCACACGTCTGAACTCCA	17	0.42500000000000004	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGTC	17	0.42500000000000004	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACAC	15	0.375	No Hit
TGAGATGAAGCACTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCC	15	0.375	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACTC	14	0.35000000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTAAGATCGGAAGAGCACACGTCTGAACTCC	12	0.3	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTAGATCGGAAGAGCACA	12	0.3	No Hit
TTCAAGTAATCCAGGATAGGCAAGATCGGAAGAGCACACGTCTGAACTCCA	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATC	11	0.27499999999999997	TruSeq Adapter, Index 9 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC	11	0.27499999999999997	No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACGT	10	0.25	No Hit
TTCACAGTGGCTAAGTTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAG	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGAATGTAAAGAAGTATGTATAGATCGGAAGAGCACACGTCTGAACTCCA	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC	10	0.25	No Hit
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCAG	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCCA	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT	9	0.22499999999999998	TruSeq Adapter, Index 9 (100% over 36bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCCA	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAAGATCGGAAGAGCACACGT	9	0.22499999999999998	No Hit
TGAGATGAAGCACTGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTCC	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAAGATCGGAAGAGCACA	8	0.2	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCGAGATCGGAAGAGCACACGT	8	0.2	No Hit
TGAGATGAAGCACGGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACTC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCCA	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGT	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTCAGATCGGAAGAGCACACGTCTGAACTCC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGATCGGAAGAGCACAC	7	0.17500000000000002	No Hit
TTCACAGTGGCTAAGTTCTGTAGATCGGAAGAGCACACGTCTGAACTCCAG	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGATGAAGCACTGTAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCAC	7	0.17500000000000002	No Hit
TGTAAACATCCTCGACTGGAAAGATCGGAAGAGCACACGTCTGAACTCCAG	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGCTTATCAGACTGATGTTGAAAGATCGGAAGAGCACACGTCTGAACTCC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGTGCTGTAGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCCCAGATCGGAAGAGCACACGTCTGAACTCCAG	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGAAAGTATGCAAGAACCAGATCGGAAGAGCACACGTCTGAACTCCAGTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGGCTTAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTCAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAGAGCACACG	5	0.125	No Hit
ACAGTAGTCTGCACATTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAG	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGCAAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAGCACCATCTGAAATCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAG	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTCT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGGTAGTAGTTTGTGCTGTAAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.025	0.0	0.0	0.0	0.0
3	0.025	0.0	0.0	0.0	0.0
4	0.025	0.0	0.0	0.0	0.0
5	0.05	0.0	0.0	0.0	0.0
6	0.075	0.0	0.0	0.0	0.0
7	0.175	0.0	0.0	0.0	0.0
8	0.475	0.0	0.0	0.0	0.0
9	0.75	0.0	0.0	0.0	0.0
10	1.075	0.0	0.0	0.0	0.0
11	1.675	0.0	0.0	0.0	0.0
12	2.45	0.0	0.0	0.0	0.0
13	3.225	0.0	0.0	0.0	0.0
14	4.325	0.0	0.0	0.0	0.0
15	5.675	0.0	0.0	0.0	0.0
16	6.25	0.0	0.0	0.0	0.0
17	7.3	0.0	0.0	0.0	0.0
18	7.85	0.0	0.0	0.0	0.0
19	8.475	0.0	0.0	0.0	0.0
20	10.25	0.0	0.0	0.0	0.0
21	16.025	0.0	0.0	0.0	0.0
22	30.3	0.0	0.0	0.0	0.0
23	65.675	0.0	0.0	0.0	0.0
24	72.875	0.0	0.0	0.0	0.0
25	74.675	0.0	0.0	0.0	0.0
26	75.025	0.0	0.0	0.0	0.0
27	75.225	0.0	0.0	0.0	0.0
28	75.5	0.0	0.0	0.0	0.0
29	75.725	0.0	0.0	0.0	0.0
30	76.025	0.0	0.0	0.0	0.0
31	77.225	0.0	0.0	0.0	0.0
32	81.4	0.0	0.0	0.0	0.0
33	90.425	0.0	0.0	0.0	0.0
34	91.775	0.0	0.0	0.0	0.0
35	93.35	0.0	0.0	0.0	0.0
36	95.1	0.0	0.0	0.0	0.0
37	95.6	0.0	0.0	0.0	0.0
38	96.075	0.0	0.0	0.0	0.0
39	96.425	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTCGCC	65	0.0	45.000004	25
GTAGCTC	130	0.0	45.000004	15
CTGTAGC	165	0.0	45.000004	13
ATGGGTG	20	6.222983E-4	45.0	3
GTAGAAT	80	0.0	45.0	18
ACTGTAG	170	0.0	45.0	12
TGGTTCA	80	0.0	45.0	8
AGCTCTA	70	0.0	45.0	17
AGCTCCA	25	3.236105E-5	45.0	17
AATTCTC	80	0.0	45.0	22
TGGTAGA	80	0.0	45.0	16
AGTGGTA	80	0.0	45.0	14
GAAGCAC	170	0.0	45.0	7
TAGCTCT	75	0.0	45.0	16
TAGCTCC	25	3.236105E-5	45.0	16
CTCCAGA	25	3.236105E-5	45.0	19
ATGAAGC	175	0.0	45.0	5
CACTGTA	170	0.0	45.0	11
GGTTCAG	80	0.0	45.0	9
GTTCAGT	80	0.0	45.0	10
>>END_MODULE
Rejected 2346134 READS because READLEN < 1
Read 2346134 spots for SRR14127851.sra
Written 2346134 spots for SRR14127851.sra
Rejected 2346134 READS because READLEN < 1
Read 2346134 spots for SRR14127851.sra
Written 2346134 spots for SRR14127851.sra
Rejected 2346134 READS because READLEN < 1
Read 2346134 spots for SRR14127851.sra
Written 2346134 spots for SRR14127851.sra
Rejected 2346138 READS because READLEN < 1
Read 2346138 spots for SRR14127851.sra
Written 2346138 spots for SRR14127851.sra
Rejected 2346134 READS because READLEN < 1
Read 2346134 spots for SRR14127851.sra
Written 2346134 spots for SRR14127851.sra
Rejected 2346134 READS because READLEN < 1
Read 2346134 spots for SRR14127851.sra
Written 2346134 spots for SRR14127851.sra
Rejected 2346134 READS because READLEN < 1
Read 2346134 spots for SRR14127851.sra
Written 2346134 spots for SRR14127851.sra
Rejected 2346134 READS because READLEN < 1
Read 2346134 spots for SRR14127851.sra
Written 2346134 spots for SRR14127851.sra
SRR ids: ['SRR14127851.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_07ak0csd
SRR14127851.sra spots: 18769076
blocks: [[1, 2346134], [2346135, 4692268], [4692269, 7038402], [7038403, 9384536], [9384537, 11730670], [11730671, 14076804], [14076805, 16422938], [16422939, 18769076]]
SRR14127851 file size 2672692
SRR14127851 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR14127851 SRR14127851_1.fastq
Input file:	SRR14127851_1.fastq
trimmed:	SRR14127851-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Tue Dec  2 20:28:28 2025 >> started

Tue Dec  2 20:28:33 2025 >> done (5.896s)
18769076 reads processed; of these:
 1421688 ( 7.57%) short reads filtered out after trimming by size control
    4328 ( 0.02%) empty reads filtered out after trimming by size control
17343060 (92.40%) reads available; of these:
  456496 ( 2.63%) trimmed reads available after processing
16886564 (97.37%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  120098	  0.69%
 19	  336398	  1.94%
 20	       0	  0.00%
 21	       0	  0.00%
 22	       0	  0.00%
 23	       0	  0.00%
 24	       0	  0.00%
 25	       0	  0.00%
 26	       0	  0.00%
 27	       0	  0.00%
 28	       0	  0.00%
 29	       0	  0.00%
 30	       0	  0.00%
 31	       0	  0.00%
 32	       0	  0.00%
 33	       0	  0.00%
 34	       0	  0.00%
 35	       0	  0.00%
 36	       0	  0.00%
 37	       0	  0.00%
 38	       0	  0.00%
 39	       0	  0.00%
 40	       0	  0.00%
 41	       0	  0.00%
 42	       0	  0.00%
 43	       0	  0.00%
 44	       0	  0.00%
 45	       0	  0.00%
 46	       0	  0.00%
 47	       0	  0.00%
 48	       0	  0.00%
 49	       0	  0.00%
 50	       0	  0.00%
 51	16886564	 97.37%
17343060 reads passed initial QC


criterion=sequence-density
sequence-density=93.96
sequence-density-rank=1
fanout-score=13.81
fanout-score-rank=6
prefix-density=95.13
prefix-fanout=13.6
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCT


criterion=fanout-score
sequence-density=1.18
sequence-density-rank=15
fanout-score=41.74
fanout-score-rank=1
prefix-density=48.09
prefix-fanout=1.0
sequence=AGCACTGTAGCGCTAGATCGG
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCT -o SRR14127851 -
Input file:	STDIN
trimmed:	SRR14127851-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Tue Dec  2 20:29:14 2025 >> started

Tue Dec  2 20:29:25 2025 >> done (10.708s)
16974059 reads processed; of these:
    8219 ( 0.05%) short reads filtered out after trimming by size control
       9 ( 0.00%) empty reads filtered out after trimming by size control
16965831 (99.95%) reads available; of these:
16440834 (96.91%) trimmed reads available after processing
  524997 ( 3.09%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  115835	  0.68%
 19	  333936	  1.97%
 20	 1057810	  6.23%
 21	 2715030	 16.00%
 22	 6734892	 39.70%
 23	 1322695	  7.80%
 24	  370931	  2.19%
 25	   71758	  0.42%
 26	   50804	  0.30%
 27	   55325	  0.33%
 28	   58941	  0.35%
 29	   58926	  0.35%
 30	  211888	  1.25%
 31	  781157	  4.60%
 32	 1694777	  9.99%
 33	  205798	  1.21%
 34	  241288	  1.42%
 35	  387717	  2.29%
 36	   96294	  0.57%
 37	   61682	  0.36%
 38	   54397	  0.32%
 39	   46497	  0.27%
 40	   26795	  0.16%
 41	   16595	  0.10%
 42	   16396	  0.10%
 43	   22001	  0.13%
 44	   33831	  0.20%
 45	   22367	  0.13%
 46	    7071	  0.04%
 47	    3320	  0.02%
 48	    3748	  0.02%
 49	       0	  0.00%
 50	       0	  0.00%
 51	   85329	  0.50%


criterion=sequence-density
sequence-density=12.22
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=11
prefix-density=0.00
prefix-fanout=1.0
sequence=GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGC


criterion=fanout-score
sequence-density=0.23
sequence-density-rank=29
fanout-score=73.43
fanout-score-rank=1
prefix-density=16.46
prefix-fanout=1.0
sequence=AGAATTCTCGCTT
                                 Started job on |	Dec 02 20:29:45
                             Started mapping on |	Dec 02 20:29:45
                                    Finished on |	Dec 02 20:31:02
       Mapping speed, Million of reads per hour |	810.46

                          Number of input reads |	17334832
                      Average input read length |	25
                                    UNIQUE READS:
                   Uniquely mapped reads number |	11088336
                        Uniquely mapped reads % |	63.97%
                          Average mapped length |	21.31
                       Number of splices: Total |	11100
            Number of splices: Annotated (sjdb) |	5001
                       Number of splices: GT/AG |	10901
                       Number of splices: GC/AG |	62
                       Number of splices: AT/AC |	16
               Number of splices: Non-canonical |	121
                      Mismatch rate per base, % |	0.11%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.31
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.33
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	3430143
             % of reads mapped to multiple loci |	19.79%
        Number of reads mapped to too many loci |	2362432
             % of reads mapped to too many loci |	13.63%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	2.58%
                     % of reads unmapped: other |	0.03%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	2816353	2816353	2816353
N_multimapping	3430143	3430143	3430143
N_noFeature	362975	632941	10807872
N_ambiguous	88430	77910	26
UnstrandedReadsAssigned:10636931 PositiveStrandReadsAssigned:10377485 NegativeStrandReadsAssigned:280438
Dataset is classified positive stranded
MeadianReadLen=22 20thPercentileLength=21 echo kmer=19
SRR14127851 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 31,715
[index] number of k-mers: 49,362,984
[index] number of equivalence classes: 166,181
[quant] running in single-end mode
[quant] will process file 1: SRR14127851-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 17,334,832 reads, 293,109 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 817 rounds

  31716 SRR14127851.ke.tsv
  32883 SRR14127851.se.tsv
  64599 total
==> SRR14127851.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	856	4502	11619.2
ENSRNOT00000040993.4	1039	940	4580	10764.2
ENSRNOT00000050156.3	1545	1446	16315	24926.5
ENSRNOT00000043693.3	684	585	3950	14917.1
ENSRNOT00000046201.3	204	105	904	19020.5
ENSRNOT00000046108.3	681	582	4542	17241.2
ENSRNOT00000049683.3	784	685	4350	14029.5
ENSRNOT00000041241.3	348	249	460	4081.33
ENSRNOT00000044582.3	297	198	864	9640.33

==> SRR14127851.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	0
ENSRNOG00000058808	0
ENSRNOG00000061316	5
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	0
SRR14127851 completed mapping pipeline successfully
