Starting /dee2/code/volunteer_pipeline.sh SRR14127849
    current disk space = 758670008
    free memory = 91309756 
SRR14127849 SRAfilesize
55e738e6fbb5d37f1bd612c552196fa0  SRR14127849.sra
SRR14127849.sra file validated
SRR14127849 is single end
SRR14127849 is conventional basespace
SRR14127849 read1 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR14127849_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.3325	37.0	37.0	37.0	37.0	37.0
2	35.889	37.0	37.0	37.0	37.0	37.0
3	36.645	37.0	37.0	37.0	37.0	37.0
4	36.363	37.0	37.0	37.0	37.0	37.0
5	36.597	37.0	37.0	37.0	37.0	37.0
6	36.4765	37.0	37.0	37.0	37.0	37.0
7	36.3985	37.0	37.0	37.0	37.0	37.0
8	36.6765	37.0	37.0	37.0	37.0	37.0
9	36.7085	37.0	37.0	37.0	37.0	37.0
10	36.44	37.0	37.0	37.0	37.0	37.0
11	36.5705	37.0	37.0	37.0	37.0	37.0
12	36.79	37.0	37.0	37.0	37.0	37.0
13	36.6395	37.0	37.0	37.0	37.0	37.0
14	36.4835	37.0	37.0	37.0	37.0	37.0
15	36.5865	37.0	37.0	37.0	37.0	37.0
16	36.5215	37.0	37.0	37.0	37.0	37.0
17	36.623	37.0	37.0	37.0	37.0	37.0
18	36.363	37.0	37.0	37.0	37.0	37.0
19	36.4885	37.0	37.0	37.0	37.0	37.0
20	36.411	37.0	37.0	37.0	37.0	37.0
21	36.4735	37.0	37.0	37.0	37.0	37.0
22	36.506	37.0	37.0	37.0	37.0	37.0
23	36.3405	37.0	37.0	37.0	37.0	37.0
24	36.3115	37.0	37.0	37.0	37.0	37.0
25	36.595	37.0	37.0	37.0	37.0	37.0
26	36.5145	37.0	37.0	37.0	37.0	37.0
27	36.516	37.0	37.0	37.0	37.0	37.0
28	36.3985	37.0	37.0	37.0	37.0	37.0
29	36.512	37.0	37.0	37.0	37.0	37.0
30	36.749	37.0	37.0	37.0	37.0	37.0
31	36.573	37.0	37.0	37.0	37.0	37.0
32	36.4825	37.0	37.0	37.0	37.0	37.0
33	36.654	37.0	37.0	37.0	37.0	37.0
34	36.4255	37.0	37.0	37.0	37.0	37.0
35	36.6395	37.0	37.0	37.0	37.0	37.0
36	36.6705	37.0	37.0	37.0	37.0	37.0
37	36.625	37.0	37.0	37.0	37.0	37.0
38	36.7075	37.0	37.0	37.0	37.0	37.0
39	36.586	37.0	37.0	37.0	37.0	37.0
40	36.2355	37.0	37.0	37.0	37.0	37.0
41	36.406	37.0	37.0	37.0	37.0	37.0
42	36.5225	37.0	37.0	37.0	37.0	37.0
43	36.4045	37.0	37.0	37.0	37.0	37.0
44	36.401	37.0	37.0	37.0	37.0	37.0
45	36.826	37.0	37.0	37.0	37.0	37.0
46	36.796	37.0	37.0	37.0	37.0	37.0
47	36.606	37.0	37.0	37.0	37.0	37.0
48	36.5115	37.0	37.0	37.0	37.0	37.0
49	36.525	37.0	37.0	37.0	37.0	37.0
50	36.5965	37.0	37.0	37.0	37.0	37.0
51	36.5845	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	1.0
26	3.0
27	5.0
28	9.0
29	17.0
30	24.0
31	42.0
32	50.0
33	55.0
34	84.0
35	150.0
36	924.0
37	2635.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	6.875000000000001	5.025	85.45	2.65
2	69.50729009552539	8.798391151332329	7.315233785822022	14.37908496732026
3	6.65	77.925	3.2750000000000004	12.15
4	75.52499999999999	7.95	5.825	10.7
5	11.35	66.825	14.575	7.249999999999999
6	18.975	4.775	72.875	3.375
7	68.8	11.425	9.6	10.174999999999999
8	12.225	71.55	14.649999999999999	1.575
9	10.65	64.775	14.149999999999999	10.424999999999999
10	68.425	15.25	13.075000000000001	3.25
11	13.225000000000001	13.750000000000002	8.975	64.05
12	6.1	66.225	11.575000000000001	16.1
13	3.225	15.174999999999999	10.525	71.075
14	14.575	11.075	69.0	5.35
15	80.825	13.575000000000001	3.65	1.95
16	4.375	16.8	75.14999999999999	3.675
17	9.6	70.0	9.125	11.275
18	66.45	7.35	20.200000000000003	6.0
19	7.9750000000000005	8.75	24.325	58.95
20	16.475	7.6	70.89999999999999	5.025
21	17.95	12.9	14.85	54.300000000000004
22	11.85	26.5	52.400000000000006	9.25
23	18.05	72.675	5.775	3.5000000000000004
24	60.9	24.25	12.875	1.975
25	9.5	61.95	20.025000000000002	8.525
26	10.725	9.125	60.475	19.675
27	24.825	3.95	10.7	60.525
28	76.625	9.675	2.375	11.325000000000001
29	71.375	24.65	1.0999999999999999	2.875
30	17.45	76.525	1.2	4.825
31	19.35	77.075	1.05	2.5250000000000004
32	69.89999999999999	26.85	1.0999999999999999	2.15
33	24.7	64.8	1.25	9.25
34	61.525	17.25	3.4250000000000003	17.8
35	8.774999999999999	18.7	2.375	70.15
36	5.525	68.2	0.8250000000000001	25.45
37	5.875	24.474999999999998	1.225	68.425
38	10.075000000000001	64.55	1.5	23.875
39	16.900000000000002	12.775	8.425	61.9
40	63.075	4.5	16.75	15.675
41	10.674999999999999	4.45	66.75	18.125
42	11.774999999999999	4.7	23.05	60.475
43	18.025	10.0	60.575	11.4
44	59.325	27.375	8.075000000000001	5.225
45	8.175	77.375	2.5	11.95
46	2.825	69.025	8.35	19.8
47	1.3	11.375	16.650000000000002	70.675
48	3.7249999999999996	4.05	59.475	32.75
49	3.1	9.25	10.475	77.17500000000001
50	8.7	16.925	4.375	70.0
51	17.0	60.150000000000006	10.725	12.125
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.5
26	0.5
27	0.0
28	0.5
29	1.0
30	0.5
31	0.0
32	0.0
33	0.0
34	1.5
35	3.0
36	2.5
37	2.0
38	4.0
39	6.0
40	19.5
41	33.0
42	92.5
43	152.0
44	154.5
45	157.0
46	389.0
47	621.0
48	1198.5
49	1776.0
50	1350.0
51	924.0
52	568.0
53	212.0
54	139.0
55	66.0
56	45.0
57	24.0
58	20.0
59	16.0
60	8.5
61	1.0
62	1.5
63	2.0
64	2.0
65	2.0
66	1.5
67	1.0
68	0.5
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.5499999999999999
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.74233128834355	15.024999999999999
2	10.797546012269938	4.3999999999999995
3	4.785276073619632	2.9250000000000003
4	2.331288343558282	1.9
5	1.4723926380368098	1.5
6	1.3496932515337423	1.6500000000000001
7	0.7361963190184049	1.05
8	0.245398773006135	0.4
9	0.49079754601227	0.8999999999999999
>10	3.067484662576687	14.875
>50	0.36809815950920244	5.800000000000001
>100	0.49079754601227	21.475
>500	0.0	0.0
>1k	0.1226993865030675	28.1
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA	1124	28.1	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG	309	7.725	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCCA	216	5.4	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCCAGATCGGAAGAGCACACGTCTGAACTCCA	170	4.25	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT	164	4.1000000000000005	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCCA	88	2.1999999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCCA	78	1.95	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCCA	66	1.6500000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAG	49	1.225	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTTAGATCGGAAGAGCACACGTCTGAACTCC	43	1.075	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT	40	1.0	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCCAGTTTTCCCAGGAATCCCTAGATCGGAAGAGCACACGTCTGAACTCC	39	0.975	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGT	37	0.9249999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCCA	36	0.8999999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG	35	0.8750000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCCA	33	0.8250000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC	30	0.75	No Hit
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCCA	27	0.675	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCAG	23	0.575	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCAGTGCACTACAGAACTTTGCAGATCGGAAGAGCACACGTCTGAACTCCA	23	0.575	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAATGTAAAGAAGTATGTATAGATCGGAAGAGCACACGTCTGAACTCCA	19	0.475	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTCC	18	0.44999999999999996	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCCA	18	0.44999999999999996	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCGAGATCGGAAGAGCACACGTCTGAACTCCA	18	0.44999999999999996	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC	15	0.375	No Hit
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCCA	13	0.325	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTATGGTTAGATCGGAAGAGCACACGTCTGAACTCCA	13	0.325	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAG	12	0.3	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGATGAGGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA	12	0.3	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCAAGATCGGAAGAGCACACGTCTGAACTCCAGT	12	0.3	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTCT	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAGATGAAGCACTGTAGCTCTCAGATCGGAAGAGCACACGTCTGAACTCC	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGCACTGATGAAACTAGAAATAGGAAGTGCCGTCCGAAACGACAACTGACG	10	0.25	No Hit
TGGAATGTAAAGAAGTATGTAGATCGGAAGAGCACACGTCTGAACTCCAGT	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCAGATCGGAAGAGCACACGTCTGAACTCCAG	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGATGAAGCACTGTAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCACATTGCCAGGGATTACCACTAGATCGGAAGAGCACACGTCTGAACTC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACCCTGTAGATCCGAATTTGTGAGATCGGAAGAGCACACGTCTGAACTCC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACCGTGAGTAATAATGTAGATCGGAAGAGCACACGTCTGAACTCCAG	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TACCCTGTAGATCCGAATTTGCAGATCGGAAGAGCACACGTCTGAACTCCA	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGAGATCGGAAGAGCACACGTCTGAACTCCAGT	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACAGTAGTCTGCACATTGGTTAGATCGGAAGAGCACACGTCTGAACTCCAG	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCAAGTAATTCAGGATAGGTTAGATCGGAAGAGCACACGTCTGAACTCCA	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACTC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTCC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTAAGATCGGAAGAGCACACGTCTGAACTCC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGATCTTGTGAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAATGTAAAGAAGTGTGTATAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCAAGTAATCCAGGATAGGAGATCGGAAGAGCACACGTCTGAACTCCAGT	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGATGAAGCACTGTAGCTTTAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGTACCGTGAGTAATAATGAGATCGGAAGAGCACACGTCTGAACTCCAGT	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGATGAAGCACTGTGGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAATGTAAAGAAGTATGTACAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCGCTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTTTAGATCGGAAGAGCACACGTCTGAACTC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTCAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTAGATCGGAAGAGCACACGTCTGAACTCC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGGCAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAACACTGTCTGGTAACGATGTAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGGAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TNAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGGTAGTAGATTGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.025	0.0	0.0	0.0	0.0
9	0.025	0.0	0.0	0.0	0.0
10	0.1	0.0	0.0	0.0	0.0
11	0.125	0.0	0.0	0.0	0.0
12	0.25	0.0	0.0	0.0	0.0
13	0.3	0.0	0.0	0.0	0.0
14	0.5	0.0	0.0	0.0	0.0
15	0.575	0.0	0.0	0.0	0.0
16	0.775	0.0	0.0	0.0	0.0
17	1.0	0.0	0.0	0.0	0.0
18	1.2	0.0	0.0	0.0	0.0
19	1.675	0.0	0.0	0.0	0.0
20	2.55	0.0	0.0	0.0	0.0
21	9.875	0.0	0.0	0.0	0.0
22	24.95	0.0	0.0	0.0	0.0
23	83.425	0.0	0.0	0.0	0.0
24	90.15	0.0	0.0	0.0	0.0
25	91.55	0.0	0.0	0.0	0.0
26	91.8	0.0	0.0	0.0	0.0
27	91.825	0.0	0.0	0.0	0.0
28	92.0	0.0	0.0	0.0	0.0
29	92.4	0.0	0.0	0.0	0.0
30	92.775	0.0	0.0	0.0	0.0
31	95.15	0.0	0.0	0.0	0.0
32	96.725	0.0	0.0	0.0	0.0
33	96.925	0.0	0.0	0.0	0.0
34	97.125	0.0	0.0	0.0	0.0
35	97.3	0.0	0.0	0.0	0.0
36	97.375	0.0	0.0	0.0	0.0
37	97.475	0.0	0.0	0.0	0.0
38	97.5	0.0	0.0	0.0	0.0
39	97.6	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCTCT	130	0.0	45.000004	16
GAGGTAG	40	4.3564796E-9	45.0	2
GTAGAAT	20	6.222983E-4	45.0	18
TGGTTCA	20	6.222983E-4	45.0	8
CATTGGT	20	6.222983E-4	45.0	2
AGCTCAG	40	4.3564796E-9	45.0	17
TTGTATA	20	6.222983E-4	45.0	13
AATTCTC	20	6.222983E-4	45.0	22
TGGTAGA	20	6.222983E-4	45.0	16
ATTGGTG	20	6.222983E-4	45.0	3
AGTGGTA	20	6.222983E-4	45.0	14
TAGCTCA	50	1.0913936E-11	45.0	16
GGTTCAG	20	6.222983E-4	45.0	9
GTTCAGT	20	6.222983E-4	45.0	10
AGAATTC	20	6.222983E-4	45.0	20
GTATAGT	20	6.222983E-4	45.0	15
TCAGTGG	20	6.222983E-4	45.0	12
TTCTCGC	20	6.222983E-4	45.0	24
TGTAGCT	205	0.0	45.0	14
GGTAGTA	40	4.3564796E-9	45.0	4
>>END_MODULE
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496737 READS because READLEN < 1
Read 496737 spots for SRR14127849.sra
Written 496737 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
Rejected 496708 READS because READLEN < 1
Read 496708 spots for SRR14127849.sra
Written 496708 spots for SRR14127849.sra
SRR ids: ['SRR14127849.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_juzavcgy
SRR14127849.sra spots: 15894685
blocks: [[1, 496708], [496709, 993416], [993417, 1490124], [1490125, 1986832], [1986833, 2483540], [2483541, 2980248], [2980249, 3476956], [3476957, 3973664], [3973665, 4470372], [4470373, 4967080], [4967081, 5463788], [5463789, 5960496], [5960497, 6457204], [6457205, 6953912], [6953913, 7450620], [7450621, 7947328], [7947329, 8444036], [8444037, 8940744], [8940745, 9437452], [9437453, 9934160], [9934161, 10430868], [10430869, 10927576], [10927577, 11424284], [11424285, 11920992], [11920993, 12417700], [12417701, 12914408], [12914409, 13411116], [13411117, 13907824], [13907825, 14404532], [14404533, 14901240], [14901241, 15397948], [15397949, 15894685]]
SRR14127849 file size 2260060
SRR14127849 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -l 18 -q 10 -k inf -t 32 -o SRR14127849 SRR14127849_1.fastq
Input file:	SRR14127849_1.fastq
trimmed:	SRR14127849-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ (auto detected)
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	32
Mon Jan 10 17:31:52 2022 >> started

Mon Jan 10 17:32:06 2022 >> done (13.893s)
15894685 reads processed; of these:
  209038 ( 1.32%) short reads filtered out after trimming by size control
    6728 ( 0.04%) empty reads filtered out after trimming by size control
15678919 (98.64%) reads available; of these:
  194322 ( 1.24%) trimmed reads available after processing
15484597 (98.76%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	   64096	  0.41%
 19	  130226	  0.83%
 20	       0	  0.00%
 21	       0	  0.00%
 22	       0	  0.00%
 23	       0	  0.00%
 24	       0	  0.00%
 25	       0	  0.00%
 26	       0	  0.00%
 27	       0	  0.00%
 28	       0	  0.00%
 29	       0	  0.00%
 30	       0	  0.00%
 31	       0	  0.00%
 32	       0	  0.00%
 33	       0	  0.00%
 34	       0	  0.00%
 35	       0	  0.00%
 36	       0	  0.00%
 37	       0	  0.00%
 38	       0	  0.00%
 39	       0	  0.00%
 40	       0	  0.00%
 41	       0	  0.00%
 42	       0	  0.00%
 43	       0	  0.00%
 44	       0	  0.00%
 45	       0	  0.00%
 46	       0	  0.00%
 47	       0	  0.00%
 48	       0	  0.00%
 49	       0	  0.00%
 50	       0	  0.00%
 51	15484597	 98.76%
15678919 reads passed initial QC


criterion=sequence-density
sequence-density=22.88
sequence-density-rank=1
fanout-score=4.77
fanout-score-rank=6
prefix-density=22.99
prefix-fanout=4.7
sequence=CAGATCGGAAGA


criterion=fanout-score
sequence-density=3.44
sequence-density-rank=6
fanout-score=14.14
fanout-score-rank=1
prefix-density=3.42
prefix-fanout=14.1
sequence=GAGATCGGAAGA
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	Jan 10 17:33:36
                             Started mapping on |	Jan 10 17:33:37
                                    Finished on |	Jan 10 17:36:48
       Mapping speed, Million of reads per hour |	295.52

                          Number of input reads |	15678919
                      Average input read length |	50
                                    UNIQUE READS:
                   Uniquely mapped reads number |	234162
                        Uniquely mapped reads % |	1.49%
                          Average mapped length |	34.29
                       Number of splices: Total |	8007
            Number of splices: Annotated (sjdb) |	4529
                       Number of splices: GT/AG |	7859
                       Number of splices: GC/AG |	41
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	106
                      Mismatch rate per base, % |	0.67%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.14
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.29
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	155575
             % of reads mapped to multiple loci |	0.99%
        Number of reads mapped to too many loci |	13379
             % of reads mapped to too many loci |	0.09%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	97.42%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	15289182	15289182	15289182
N_multimapping	155575	155575	155575
N_noFeature	25351	31271	227042
N_ambiguous	5095	3893	2
UnstrandedReadsAssigned:203716 PositiveStrandReadsAssigned:198998 NegativeStrandReadsAssigned:7118
Dataset is classified positive stranded
MeadianReadLen=51 20thPercentileLength=51 echo kmer=47
SRR14127849 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 31,715
[index] number of k-mers: 49,915,185
[index] number of equivalence classes: 70,351
[quant] running in single-end mode
[quant] will process file 1: SRR14127849-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 15,678,919 reads, 34,510 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 788 rounds

  31716 SRR14127849.ke.tsv
  32883 SRR14127849.se.tsv
  64599 total
==> SRR14127849.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	856	22	1263.34
ENSRNOT00000040993.4	1039	940	31	1621.09
ENSRNOT00000050156.3	1545	1446	148	5031.13
ENSRNOT00000043693.3	684	585	59	4957.56
ENSRNOT00000046201.3	204	105	5	2340.74
ENSRNOT00000046108.3	681	582	31	2618.25
ENSRNOT00000049683.3	784	685	50	3587.99
ENSRNOT00000041241.3	348	249	11	2171.53
ENSRNOT00000044582.3	297	198	13	3227.38

==> SRR14127849.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	0
ENSRNOG00000058808	0
ENSRNOG00000061316	1
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	0
SRR14127849 completed mapping pipeline successfully
