Starting /dee2/code/volunteer_pipeline.sh SRR17096855
    current disk space = 451770636
    free memory = 196887888 
SRR17096855 SRAfilesize
2b68808b1871c82e005fe367e96f95cf  SRR17096855.sra
SRR17096855.sra file validated
SRR17096855 is single end
SRR17096855 is conventional basespace
SRR17096855 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR17096855_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13625	32.0	32.0	32.0	32.0	32.0
2	30.94175	32.0	32.0	32.0	32.0	32.0
3	30.99	32.0	32.0	32.0	32.0	32.0
4	31.401	32.0	32.0	32.0	32.0	32.0
5	31.36425	32.0	32.0	32.0	32.0	32.0
6	34.59275	36.0	36.0	36.0	32.0	36.0
7	34.21725	36.0	36.0	36.0	32.0	36.0
8	34.5825	36.0	36.0	36.0	32.0	36.0
9	34.47925	36.0	36.0	36.0	32.0	36.0
10	35.14275	36.0	36.0	36.0	36.0	36.0
11	35.08275	36.0	36.0	36.0	36.0	36.0
12	34.67075	36.0	36.0	36.0	32.0	36.0
13	35.06025	36.0	36.0	36.0	36.0	36.0
14	34.8115	36.0	36.0	36.0	32.0	36.0
15	34.828	36.0	36.0	36.0	36.0	36.0
16	34.863	36.0	36.0	36.0	36.0	36.0
17	34.9405	36.0	36.0	36.0	36.0	36.0
18	35.01125	36.0	36.0	36.0	36.0	36.0
19	34.75125	36.0	36.0	36.0	32.0	36.0
20	34.956	36.0	36.0	36.0	36.0	36.0
21	34.801	36.0	36.0	36.0	32.0	36.0
22	34.64425	36.0	36.0	36.0	32.0	36.0
23	34.60875	36.0	36.0	36.0	32.0	36.0
24	34.5635	36.0	36.0	36.0	32.0	36.0
25	34.466	36.0	36.0	36.0	32.0	36.0
26	34.43075	36.0	36.0	36.0	32.0	36.0
27	34.468	36.0	36.0	36.0	32.0	36.0
28	34.49025	36.0	36.0	36.0	32.0	36.0
29	34.5775	36.0	36.0	36.0	32.0	36.0
30	34.786	36.0	36.0	36.0	36.0	36.0
31	34.28725	36.0	36.0	36.0	32.0	36.0
32	34.7585	36.0	36.0	36.0	36.0	36.0
33	34.40975	36.0	36.0	36.0	32.0	36.0
34	34.42275	36.0	36.0	36.0	32.0	36.0
35	34.591	36.0	36.0	36.0	32.0	36.0
36	34.55775	36.0	36.0	36.0	32.0	36.0
37	34.15625	36.0	36.0	36.0	32.0	36.0
38	34.3575	36.0	36.0	36.0	32.0	36.0
39	34.18475	36.0	36.0	36.0	32.0	36.0
40	34.25	36.0	36.0	36.0	32.0	36.0
41	34.091	36.0	36.0	36.0	32.0	36.0
42	34.04625	36.0	36.0	36.0	32.0	36.0
43	33.97375	36.0	36.0	36.0	32.0	36.0
44	33.8865	36.0	36.0	36.0	32.0	36.0
45	34.10175	36.0	36.0	36.0	32.0	36.0
46	33.297	36.0	36.0	36.0	21.0	36.0
47	33.9075	36.0	36.0	36.0	32.0	36.0
48	33.85675	36.0	36.0	36.0	32.0	36.0
49	33.6425	36.0	36.0	36.0	27.0	36.0
50	33.704	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	8.0
22	6.0
23	11.0
24	16.0
25	20.0
26	37.0
27	37.0
28	63.0
29	99.0
30	107.0
31	124.0
32	213.0
33	328.0
34	688.0
35	2239.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	4.7	11.525	76.875	6.9
2	73.97500000000001	6.15	9.625	10.25
3	70.05	9.85	9.55	10.549999999999999
4	13.675	74.95	5.975	5.4
5	7.925	75.875	7.825	8.375
6	9.389083625438158	11.392088132198296	75.33800701051577	3.8808212318477717
7	78.125	5.0	5.575	11.3
8	6.35	10.65	76.2	6.800000000000001
9	11.65	72.6	12.425	3.325
10	12.025	76.125	6.25	5.6000000000000005
11	10.6	69.125	11.575000000000001	8.7
12	69.25	8.425	11.799999999999999	10.525
13	5.7250000000000005	74.425	9.025	10.825
14	8.225	78.975	9.475	3.325
15	76.925	9.55	8.225	5.3
16	3.225	9.4	80.22500000000001	7.1499999999999995
17	33.35	54.675	7.85	4.125
18	10.15	13.200000000000001	71.925	4.725
19	73.0	13.025	10.35	3.6249999999999996
20	12.7	13.950000000000001	69.77499999999999	3.5749999999999997
21	15.1	72.925	7.324999999999999	4.65
22	20.9	17.05	54.175	7.875
23	10.475	73.45	7.3	8.774999999999999
24	55.300000000000004	23.125	19.225	2.35
25	14.6	57.099999999999994	12.325	15.975
26	17.95	11.425	52.925	17.7
27	22.8	6.65	12.375	58.175
28	61.925	16.75	4.625	16.7
29	65.05	24.525	5.425	5.0
30	26.474999999999998	63.324999999999996	1.675	8.525
31	16.275000000000002	77.5	1.5	4.725
32	67.4	26.700000000000003	1.875	4.025
33	19.55	59.12500000000001	1.55	19.775000000000002
34	55.574999999999996	29.375	2.6	12.45
35	10.174999999999999	15.35	3.4250000000000003	71.05
36	5.675	72.3	2.1	19.925
37	6.325	23.025000000000002	2.1999999999999997	68.45
38	22.400000000000002	57.025000000000006	2.0	18.575
39	15.299999999999999	13.775	15.475	55.45
40	57.95	6.925000000000001	11.5	23.625
41	12.0	5.0	70.275	12.725
42	18.05	5.925	22.475	53.55
43	12.85	17.5	57.825	11.825
44	56.525000000000006	25.6	10.85	7.025
45	10.299999999999999	64.95	7.3999999999999995	17.349999999999998
46	5.1499999999999995	66.675	14.975	13.200000000000001
47	5.55	16.55	10.025	67.875
48	3.2	10.0	53.0	33.800000000000004
49	3.6249999999999996	17.4	13.925	65.05
50	15.975	11.825	5.475	66.725
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.0
14	1.0
15	1.5
16	2.0
17	1.5
18	1.0
19	2.0
20	3.0
21	2.5
22	2.0
23	2.0
24	2.0
25	3.0
26	4.0
27	5.0
28	6.0
29	6.0
30	6.0
31	5.0
32	4.0
33	9.0
34	14.0
35	15.5
36	17.0
37	22.0
38	27.0
39	104.5
40	182.0
41	684.0
42	1186.0
43	1169.0
44	1152.0
45	715.0
46	278.0
47	268.5
48	259.0
49	355.5
50	452.0
51	349.5
52	247.0
53	172.0
54	97.0
55	64.5
56	32.0
57	22.0
58	12.0
59	10.5
60	9.0
61	5.5
62	2.0
63	1.5
64	1.0
65	1.0
66	1.0
67	0.5
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.15
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.22460391425909	22.325
2	6.989748369058714	3.75
3	1.863932898415657	1.5
4	1.4911463187325256	1.6
5	0.9319664492078285	1.25
6	0.7455731593662628	1.2
7	0.3727865796831314	0.7000000000000001
8	0.3727865796831314	0.8
9	0.4659832246039142	1.125
>10	2.8890959925442683	16.125
>50	0.27958993476234856	6.1
>100	0.27958993476234856	21.2
>500	0.09319664492078285	22.325
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGAATGTAAAGAAGTATGTATAGATCGGAAGAGCACACGTCTGAACTCC	893	22.325	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATGTAAAGAAGTGTGTATAGATCGGAAGAGCACACGTCTGAACTCC	403	10.075000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATGTAAAGAAGTATGTAGATCGGAAGAGCACACGTCTGAACTCCAG	328	8.200000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC	117	2.9250000000000003	No Hit
TGGAATGTAAAGAAGTGTGTAAGATCGGAAGAGCACACGTCTGAACTCCA	89	2.225	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAATGTAAAGAAGTATGTATTAGATCGGAAGAGCACACGTCTGAACTC	82	2.0500000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGAATGTAAAGAAGTATGTACAGATCGGAAGAGCACACGTCTGAACTCC	73	1.825	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATGTAAAGAAGTGTGTACAGATCGGAAGAGCACACGTCTGAACTCC	45	1.125	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATGTAAAGAAGTATGTAAGATCGGAAGAGCACACGTCTGAACTCCA	42	1.05	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAATGTAAAGAAGTGTGTATTAGATCGGAAGAGCACACGTCTGAACTC	36	0.8999999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT	32	0.8	No Hit
TGGAATGTAAAGAAGTATGTAAAGATCGGAAGAGCACACGTCTGAACTCC	29	0.7250000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG	28	0.7000000000000001	No Hit
ACTGGACTTGGAGTCAGAAGGCAGATCGGAAGAGCACACGTCTGAACTCC	27	0.675	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATGTAAAGAAGTGTGTAGATCGGAAGAGCACACGTCTGAACTCCAG	27	0.675	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGAATGTAAAGAAGTATGTATAGATCGGAAGAGCACACGTCTGAACTCC	26	0.65	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATGTAAAGAAGTATGCAGATCGGAAGAGCACACGTCTGAACTCCAG	25	0.625	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACTGGACTTGGAGTCAGAAGGAGATCGGAAGAGCACACGTCTGAACTCCA	23	0.575	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAATGTAAGGAAGTGTGTGGAGATCGGAAGAGCACACGTCTGAACTCC	23	0.575	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC	21	0.525	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC	20	0.5	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA	19	0.475	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT	19	0.475	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC	17	0.42500000000000004	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGAATGTAAAGAAGTGTGTATAGATCGGAAGAGCACACGTCTGAACTCC	17	0.42500000000000004	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCAGATCGGAAGAGCACACGTCTGAACTCCA	16	0.4	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC	15	0.375	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT	15	0.375	No Hit
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT	14	0.35000000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC	14	0.35000000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGGAAGATCGGAAGAGCACACGTCTGAACTCC	14	0.35000000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATGTAAAGAAGTATGTATAAGATCGGAAGAGCACACGTCTGAACTC	13	0.325	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGAATGTAAAGAAGTGTGTAAAGATCGGAAGAGCACACGTCTGAACTCC	12	0.3	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC	12	0.3	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG	12	0.3	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACTGGACTTGGAGTCAGAAGAGATCGGAAGAGCACACGTCTGAACTCCAG	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCAAGTAATCCAGGATAGGAGATCGGAAGAGCACACGTCTGAACTCCAG	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCTACACTCTCAGCAGATCGGAAGAGCACACGTCTGAACTC	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC	9	0.22499999999999998	No Hit
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGGTCCCCTTCAACCAGCTGTAGATCGGAAGAGCACACGTCTGAACTCC	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGTACCGTGAGTAATAATGCAGATCGGAAGAGCACACGTCTGAACTCCA	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG	8	0.2	No Hit
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGATCTTGTGAAGATCGGAAGAGCACACGTCTGAACTC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGAATGTAAAGAAGTATGTAGAGATCGGAAGAGCACACGTCTGAACTCC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATGTAAAGAAGTGTGTATAAGATCGGAAGAGCACACGTCTGAACTC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGAATGTAAAGAAGTGTGTATCAGATCGGAAGAGCACACGTCTGAACTC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTGAGATCGGAAGAGCACACGTCTGAACTC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGAATGTAAAGAAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGGAATGTAAAGAAGTATGTAGATCGGAAGAGCACACGTCTGAACTCCAG	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGAATGTAAAGAGGTATGTATAGATCGGAAGAGCACACGTCTGAACTCC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGTAGATCGGAAGAGCACACGTCTGAACTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTGGACTTGGAGTCAGAAGGCAAGATCGGAAGAGCACACGTCTGAACTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGGATGTAAAGAAGTATGTATAGATCGGAAGAGCACACGTCTGAACTCC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGAAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGCAATGTAAAGAAGTATGTATAGATCGGAAGAGCACACGTCTGAACTCC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAAAC	6	0.15	No Hit
TGGAATGTAAGGAAGTGTGTGAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAAAGTAAAGAAGTATGTATAGATCGGAAGAGCACACGTCTGAACTCC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCAC	5	0.125	No Hit
TGGAATGTAAAGAAGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGCACTGATGAAACTAGAAATAGGAAGTGCCGTCCGAAACGACAACTGAC	5	0.125	No Hit
AACCCGTAGATCCGATCTTGTGAGATCGGAAGAGCACACGTCTGAACTCC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAGTGTAAAGAAGTATGTATAGATCGGAAGAGCACACGTCTGAACTCC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAATGTAGAGAAGTATGTAGATCGGAAGAGCACACGTCTGAACTCCAG	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACTGGACTTGGAGTCAGAAGGTAGATCGGAAGAGCACACGTCTGAACTCC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.025	0.0	0.0	0.0	0.0
9	0.025	0.0	0.0	0.0	0.0
10	0.05	0.0	0.0	0.0	0.0
11	0.075	0.0	0.0	0.0	0.0
12	0.175	0.0	0.0	0.0	0.0
13	0.2	0.0	0.0	0.0	0.0
14	0.35	0.0	0.0	0.0	0.0
15	0.575	0.0	0.0	0.0	0.0
16	0.9	0.0	0.0	0.0	0.0
17	1.475	0.0	0.0	0.0	0.0
18	1.75	0.0	0.0	0.0	0.0
19	2.45	0.0	0.0	0.0	0.0
20	3.225	0.0	0.0	0.0	0.0
21	15.975	0.0	0.0	0.0	0.0
22	23.725	0.0	0.0	0.0	0.0
23	73.85	0.0	0.0	0.0	0.0
24	81.55	0.0	0.0	0.0	0.0
25	83.625	0.0	0.0	0.0	0.0
26	83.95	0.0	0.0	0.0	0.0
27	84.175	0.0	0.0	0.0	0.0
28	84.475	0.0	0.0	0.0	0.0
29	84.675	0.0	0.0	0.0	0.0
30	84.875	0.0	0.0	0.0	0.0
31	85.7	0.0	0.0	0.0	0.0
32	87.275	0.0	0.0	0.0	0.0
33	87.825	0.0	0.0	0.0	0.0
34	88.1	0.0	0.0	0.0	0.0
35	88.275	0.0	0.0	0.0	0.0
36	88.6	0.0	0.0	0.0	0.0
37	88.925	0.0	0.0	0.0	0.0
38	89.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGACT	20	6.952051E-4	44.000004	11
ATCCTCG	20	6.952051E-4	44.000004	8
AAGTATG	180	0.0	44.000004	13
TCCTCGA	20	6.952051E-4	44.000004	9
TCGACTG	20	6.952051E-4	44.000004	12
AGAAGTA	175	0.0	44.000004	11
CATCCTC	20	6.952051E-4	44.000004	7
GAAGTAT	180	0.0	44.000004	12
ACATCCT	20	6.952051E-4	44.000004	6
TCGCTGC	20	6.952051E-4	44.000004	2
TGTACAG	20	6.952051E-4	44.000004	18
CTCGCTG	20	6.952051E-4	44.000004	1
TAAACAT	25	3.6958358E-5	44.0	3
AGAAGTG	50	1.4551915E-11	44.0	11
TACAGAT	25	3.6958358E-5	44.0	20
AAACATC	25	3.6958358E-5	44.0	4
GTGTATA	25	3.6958358E-5	44.0	17
TATGTAT	105	0.0	44.0	16
GAAGTGT	55	0.0	44.0	12
GTACAGA	25	3.6958358E-5	44.0	19
>>END_MODULE
Rejected 1002548 READS because READLEN < 1
Read 1002548 spots for SRR17096855.sra
Written 1002548 spots for SRR17096855.sra
Rejected 1002548 READS because READLEN < 1
Read 1002548 spots for SRR17096855.sra
Written 1002548 spots for SRR17096855.sra
Rejected 1002548 READS because READLEN < 1
Read 1002548 spots for SRR17096855.sra
Written 1002548 spots for SRR17096855.sra
Rejected 1002555 READS because READLEN < 1
Read 1002555 spots for SRR17096855.sra
Written 1002555 spots for SRR17096855.sra
Rejected 1002548 READS because READLEN < 1
Read 1002548 spots for SRR17096855.sra
Written 1002548 spots for SRR17096855.sra
Rejected 1002548 READS because READLEN < 1
Read 1002548 spots for SRR17096855.sra
Written 1002548 spots for SRR17096855.sra
Rejected 1002548 READS because READLEN < 1
Read 1002548 spots for SRR17096855.sra
Written 1002548 spots for SRR17096855.sra
Rejected 1002548 READS because READLEN < 1
Read 1002548 spots for SRR17096855.sra
Written 1002548 spots for SRR17096855.sra
SRR ids: ['SRR17096855.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_58bj1e5n
SRR17096855.sra spots: 8020391
blocks: [[1, 1002548], [1002549, 2005096], [2005097, 3007644], [3007645, 4010192], [4010193, 5012740], [5012741, 6015288], [6015289, 7017836], [7017837, 8020391]]
SRR17096855 file size 1117868
SRR17096855 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR17096855 SRR17096855_1.fastq
Input file:	SRR17096855_1.fastq
trimmed:	SRR17096855-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Wed Nov 26 13:31:01 2025 >> started

Wed Nov 26 13:31:04 2025 >> done (2.911s)
8020391 reads processed; of these:
 171986 ( 2.14%) short reads filtered out after trimming by size control
   5381 ( 0.07%) empty reads filtered out after trimming by size control
7843024 (97.79%) reads available; of these:
  58375 ( 0.74%) trimmed reads available after processing
7784649 (99.26%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  58157	  0.74%
 19	      0	  0.00%
 20	      0	  0.00%
 21	      0	  0.00%
 22	      0	  0.00%
 23	      0	  0.00%
 24	      0	  0.00%
 25	      0	  0.00%
 26	      0	  0.00%
 27	      0	  0.00%
 28	      0	  0.00%
 29	      0	  0.00%
 30	      0	  0.00%
 31	      0	  0.00%
 32	      0	  0.00%
 33	      0	  0.00%
 34	      0	  0.00%
 35	      0	  0.00%
 36	      0	  0.00%
 37	      0	  0.00%
 38	      0	  0.00%
 39	      0	  0.00%
 40	      0	  0.00%
 41	      0	  0.00%
 42	      0	  0.00%
 43	      0	  0.00%
 44	      0	  0.00%
 45	      0	  0.00%
 46	      0	  0.00%
 47	      0	  0.00%
 48	     14	  0.00%
 49	    204	  0.00%
 50	7784649	 99.26%
7843024 reads passed initial QC


criterion=sequence-density
sequence-density=59.40
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=15
prefix-density=0.01
prefix-fanout=1.0
sequence=TGGAATGTAAAGAAGTATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=21
fanout-score=3415.24
fanout-score-rank=1
prefix-density=28.93
prefix-fanout=1.0
sequence=AGTATGTATTGTAAACATCCTCGACTGGAAGC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGGAATGTAAAGAAGTATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA -o SRR17096855 -
Input file:	STDIN
trimmed:	SRR17096855-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATGTAAAGAAGTATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Wed Nov 26 13:31:10 2025 >> started

Wed Nov 26 13:31:14 2025 >> done (4.181s)
7581590 reads processed; of these:
   3885 ( 0.05%) short reads filtered out after trimming by size control
4706872 (62.08%) empty reads filtered out after trimming by size control
2870833 (37.87%) reads available; of these:
   9793 ( 0.34%) trimmed reads available after processing
2861040 (99.66%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  33450	  1.17%
 19	      8	  0.00%
 20	     14	  0.00%
 21	     35	  0.00%
 22	    515	  0.02%
 23	     28	  0.00%
 24	     15	  0.00%
 25	      6	  0.00%
 26	      2	  0.00%
 27	      4	  0.00%
 28	      4	  0.00%
 29	      7	  0.00%
 30	      7	  0.00%
 31	      1	  0.00%
 32	      1	  0.00%
 33	      1	  0.00%
 34	      0	  0.00%
 35	      1	  0.00%
 36	      0	  0.00%
 37	      0	  0.00%
 38	      0	  0.00%
 39	      0	  0.00%
 40	      0	  0.00%
 41	      2	  0.00%
 42	      3	  0.00%
 43	      3	  0.00%
 44	      9	  0.00%
 45	    217	  0.01%
 46	   3844	  0.13%
 47	   5063	  0.18%
 48	      6	  0.00%
 49	     63	  0.00%
 50	2827524	 98.49%


criterion=sequence-density
sequence-density=73.71
sequence-density-rank=1
fanout-score=22.65
fanout-score-rank=2
prefix-density=75.68
prefix-fanout=22.1
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAA


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=9
fanout-score=23.03
fanout-score-rank=1
prefix-density=0.39
prefix-fanout=1.4
sequence=AGTATGTATTGTAAACATCCTCGACTGGAAGC
                                 Started job on |	Nov 26 13:31:22
                             Started mapping on |	Nov 26 13:31:22
                                    Finished on |	Nov 26 13:31:35
       Mapping speed, Million of reads per hour |	867.40

                          Number of input reads |	3132267
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	170700
                        Uniquely mapped reads % |	5.45%
                          Average mapped length |	43.13
                       Number of splices: Total |	9625
            Number of splices: Annotated (sjdb) |	6723
                       Number of splices: GT/AG |	9411
                       Number of splices: GC/AG |	57
                       Number of splices: AT/AC |	6
               Number of splices: Non-canonical |	151
                      Mismatch rate per base, % |	1.01%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.25
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.07
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	475668
             % of reads mapped to multiple loci |	15.19%
        Number of reads mapped to too many loci |	8441
             % of reads mapped to too many loci |	0.27%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	79.08%
                     % of reads unmapped: other |	0.01%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	2485899	2485899	2485899
N_multimapping	475668	475668	475668
N_noFeature	36027	37688	163821
N_ambiguous	9130	3887	25
UnstrandedReadsAssigned:125543 PositiveStrandReadsAssigned:129125 NegativeStrandReadsAssigned:6854
Dataset is classified positive stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR17096855 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 31,715
[index] number of k-mers: 49,915,185
[index] number of equivalence classes: 70,351
[quant] running in single-end mode
[quant] will process file 1: SRR17096855-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,132,267 reads, 62,930 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 667 rounds

  31716 SRR17096855.ke.tsv
  32883 SRR17096855.se.tsv
  64599 total
==> SRR17096855.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	856	1169	26739.9
ENSRNOT00000040993.4	1039	940	2117	44097.3
ENSRNOT00000050156.3	1545	1446	4793	64902
ENSRNOT00000043693.3	684	585	2855	95558.5
ENSRNOT00000046201.3	204	105	334	62284
ENSRNOT00000046108.3	681	582	1318	44341.6
ENSRNOT00000049683.3	784	685	2007	57368.8
ENSRNOT00000041241.3	348	249	378	29724.3
ENSRNOT00000044582.3	297	198	212	20964.7

==> SRR17096855.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	0
ENSRNOG00000058808	0
ENSRNOG00000061316	0
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	0
SRR17096855 completed mapping pipeline successfully
