Starting /dee2/code/volunteer_pipeline.sh SRR17096853
    current disk space = 423554196
    free memory = 196828060 
SRR17096853 SRAfilesize
e9ba64180bd211f30e0ab939f9eda6d1  SRR17096853.sra
SRR17096853.sra file validated
SRR17096853 is single end
SRR17096853 is conventional basespace
SRR17096853 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR17096853_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.121	32.0	32.0	32.0	32.0	32.0
2	30.9355	32.0	32.0	32.0	32.0	32.0
3	31.11275	32.0	32.0	32.0	32.0	32.0
4	31.301	32.0	32.0	32.0	32.0	32.0
5	31.4195	32.0	32.0	32.0	32.0	32.0
6	34.7	36.0	36.0	36.0	32.0	36.0
7	34.1535	36.0	36.0	36.0	32.0	36.0
8	34.66075	36.0	36.0	36.0	32.0	36.0
9	34.72525	36.0	36.0	36.0	32.0	36.0
10	35.18775	36.0	36.0	36.0	36.0	36.0
11	35.15425	36.0	36.0	36.0	36.0	36.0
12	34.776	36.0	36.0	36.0	32.0	36.0
13	35.252	36.0	36.0	36.0	36.0	36.0
14	35.0705	36.0	36.0	36.0	36.0	36.0
15	35.19	36.0	36.0	36.0	36.0	36.0
16	35.01975	36.0	36.0	36.0	36.0	36.0
17	35.07325	36.0	36.0	36.0	36.0	36.0
18	34.9755	36.0	36.0	36.0	36.0	36.0
19	34.86975	36.0	36.0	36.0	36.0	36.0
20	35.0215	36.0	36.0	36.0	36.0	36.0
21	34.94375	36.0	36.0	36.0	36.0	36.0
22	34.71325	36.0	36.0	36.0	32.0	36.0
23	34.62225	36.0	36.0	36.0	32.0	36.0
24	34.8125	36.0	36.0	36.0	32.0	36.0
25	34.62925	36.0	36.0	36.0	32.0	36.0
26	34.61125	36.0	36.0	36.0	32.0	36.0
27	34.68525	36.0	36.0	36.0	32.0	36.0
28	34.709	36.0	36.0	36.0	32.0	36.0
29	34.758	36.0	36.0	36.0	36.0	36.0
30	34.867	36.0	36.0	36.0	36.0	36.0
31	34.334	36.0	36.0	36.0	32.0	36.0
32	34.82325	36.0	36.0	36.0	36.0	36.0
33	34.39925	36.0	36.0	36.0	32.0	36.0
34	34.495	36.0	36.0	36.0	32.0	36.0
35	34.70475	36.0	36.0	36.0	32.0	36.0
36	34.619	36.0	36.0	36.0	32.0	36.0
37	34.49425	36.0	36.0	36.0	32.0	36.0
38	34.572	36.0	36.0	36.0	32.0	36.0
39	34.334	36.0	36.0	36.0	32.0	36.0
40	34.321	36.0	36.0	36.0	32.0	36.0
41	34.247	36.0	36.0	36.0	32.0	36.0
42	34.2585	36.0	36.0	36.0	32.0	36.0
43	34.26625	36.0	36.0	36.0	32.0	36.0
44	34.0535	36.0	36.0	36.0	32.0	36.0
45	34.322	36.0	36.0	36.0	32.0	36.0
46	33.557	36.0	36.0	36.0	27.0	36.0
47	34.26725	36.0	36.0	36.0	32.0	36.0
48	34.1965	36.0	36.0	36.0	32.0	36.0
49	34.07725	36.0	36.0	36.0	32.0	36.0
50	33.857	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	7.0
22	4.0
23	11.0
24	8.0
25	17.0
26	40.0
27	40.0
28	55.0
29	58.0
30	97.0
31	139.0
32	189.0
33	316.0
34	648.0
35	2370.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	7.825	10.2	76.1	5.875
2	74.575	4.7	7.95	12.775
3	71.775	10.85	8.475000000000001	8.9
4	10.95	75.64999999999999	8.35	5.050000000000001
5	8.325000000000001	76.775	9.1	5.800000000000001
6	12.107510675709621	9.294147199196182	74.83044461190656	3.7678975131876418
7	80.5	4.475	5.0	10.025
8	5.125	10.25	79.125	5.5
9	12.15	74.075	10.549999999999999	3.225
10	14.025000000000002	74.8	5.825	5.35
11	9.25	70.85000000000001	11.875	8.025
12	71.0	7.75	12.325	8.924999999999999
13	5.7250000000000005	75.1	6.675000000000001	12.5
14	8.1	80.875	7.925	3.1
15	80.325	8.5	5.7	5.475
16	3.65	9.45	80.875	6.0249999999999995
17	33.85	54.7	7.85	3.5999999999999996
18	12.55	11.175	72.225	4.05
19	74.675	11.025	10.8	3.5000000000000004
20	11.35	14.249999999999998	70.975	3.4250000000000003
21	15.075	74.925	5.8999999999999995	4.1000000000000005
22	20.175	19.05	52.5	8.275
23	11.475	74.875	6.4	7.249999999999999
24	55.275	21.575	20.925	2.225
25	13.325000000000001	55.925000000000004	14.924999999999999	15.825
26	16.625	11.525	52.949999999999996	18.9
27	22.900000000000002	6.175	14.45	56.474999999999994
28	62.324999999999996	16.3	3.4250000000000003	17.95
29	66.4	24.575	4.125	4.9
30	25.275	62.975	1.5	10.25
31	14.924999999999999	76.5	2.15	6.425
32	67.30000000000001	25.974999999999998	2.6	4.125
33	21.15	58.650000000000006	1.6500000000000001	18.55
34	55.45	29.375	3.375	11.799999999999999
35	10.274999999999999	14.7	5.25	69.77499999999999
36	5.7250000000000005	70.75	2.725	20.8
37	8.725	21.3	2.075	67.9
38	22.3	56.974999999999994	2.275	18.45
39	14.499999999999998	15.299999999999999	15.6	54.6
40	57.099999999999994	8.9	11.025	22.975
41	13.125	6.7	68.0	12.174999999999999
42	18.224999999999998	7.9	20.075000000000003	53.800000000000004
43	14.549999999999999	17.599999999999998	55.974999999999994	11.875
44	56.025000000000006	25.124999999999996	10.6	8.25
45	9.700000000000001	66.57499999999999	5.25	18.475
46	4.2	66.125	14.499999999999998	15.174999999999999
47	4.7	16.075	10.025	69.19999999999999
48	3.9	8.575000000000001	53.075	34.449999999999996
49	5.875	16.825000000000003	12.85	64.45
50	16.0	12.325	6.175	65.5
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	0.5
16	0.0
17	0.0
18	0.0
19	1.5
20	3.0
21	2.5
22	2.0
23	3.0
24	4.0
25	4.0
26	4.0
27	4.0
28	4.0
29	4.0
30	4.0
31	5.0
32	6.0
33	6.0
34	6.0
35	12.0
36	18.0
37	23.5
38	29.0
39	92.0
40	155.0
41	693.0
42	1231.0
43	1181.5
44	1132.0
45	707.0
46	282.0
47	256.0
48	230.0
49	291.5
50	353.0
51	325.5
52	298.0
53	214.5
54	131.0
55	95.0
56	59.0
57	43.0
58	27.0
59	18.5
60	10.0
61	6.5
62	3.0
63	4.0
64	5.0
65	4.0
66	3.0
67	1.5
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.475
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.25
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.38095238095238	21.625
2	7.0476190476190474	3.6999999999999997
3	2.380952380952381	1.875
4	1.6190476190476188	1.7000000000000002
5	1.0476190476190477	1.375
6	0.2857142857142857	0.44999999999999996
7	1.0476190476190477	1.925
8	0.4761904761904762	1.0
9	0.19047619047619047	0.44999999999999996
>10	2.666666666666667	13.925
>50	0.5714285714285714	11.25
>100	0.19047619047619047	17.724999999999998
>500	0.09523809523809523	23.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGAATGTAAAGAAGTATGTATAGATCGGAAGAGCACACGTCTGAACTCC	920	23.0	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATGTAAAGAAGTGTGTATAGATCGGAAGAGCACACGTCTGAACTCC	397	9.925	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATGTAAAGAAGTATGTAGATCGGAAGAGCACACGTCTGAACTCCAG	312	7.8	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGAATGTAAAGAAGTATGTACAGATCGGAAGAGCACACGTCTGAACTCC	94	2.35	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATGTAAAGAAGTGTGTAAGATCGGAAGAGCACACGTCTGAACTCCA	83	2.075	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAATGTAAAGAAGTATGTATTAGATCGGAAGAGCACACGTCTGAACTC	74	1.8499999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGAATGTAAAGAAGTATGTAAGATCGGAAGAGCACACGTCTGAACTCCA	73	1.825	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC	67	1.675	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT	59	1.4749999999999999	No Hit
TGGAATGTAAAGAAGTGTGTATTAGATCGGAAGAGCACACGTCTGAACTC	41	1.0250000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGAATGTAAAGAAGTGTGTACAGATCGGAAGAGCACACGTCTGAACTCC	41	1.0250000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG	33	0.8250000000000001	No Hit
TGGAATGTAAAGAAGTGTGTAGATCGGAAGAGCACACGTCTGAACTCCAG	32	0.8	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGAATGTAAAGAAGTATGTATAGATCGGAAGAGCACACGTCTGAACTCC	32	0.8	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGGCAGATCGGAAGAGCACACGTCTGAACTCC	31	0.775	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC	27	0.675	No Hit
ACTGGACTTGGAGTCAGAAGGAGATCGGAAGAGCACACGTCTGAACTCCA	24	0.6	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGAATGTAAGGAAGTGTGTGGAGATCGGAAGAGCACACGTCTGAACTCC	22	0.5499999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC	21	0.525	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGAATGTAAAGAAGTATGCAGATCGGAAGAGCACACGTCTGAACTCCAG	20	0.5	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGAATGTAAAGAAGTATGTAAAGATCGGAAGAGCACACGTCTGAACTCC	19	0.475	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT	19	0.475	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT	18	0.44999999999999996	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG	18	0.44999999999999996	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT	18	0.44999999999999996	No Hit
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC	15	0.375	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATGTAAAGAAGTGTGTAAAGATCGGAAGAGCACACGTCTGAACTCC	14	0.35000000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATGTAAAGAAGTATGTATAAGATCGGAAGAGCACACGTCTGAACTC	14	0.35000000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA	13	0.325	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGAATGTAAAGAAGTATGTATAGATCGGAAGAGCACACGTCTGAACTCC	12	0.3	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGAATGTAAAGAAGTATGTAGATCGGAAGAGCACACGTCTGAACTCCAG	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCTACACTCTCAGCAGATCGGAAGAGCACACGTCTGAACTC	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGAATGTAAAGAAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTGGACTTGGAGTCAGAAGAGATCGGAAGAGCACACGTCTGAACTCCAG	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAAGTAATCCAGGATAGGAGATCGGAAGAGCACACGTCTGAACTCCAG	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC	9	0.22499999999999998	No Hit
TGAGATGAAGCACTGTAGCTAGATCGGAAGAGCACACGTCTGAACTCCAG	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGTAGTAGTTTGTACAGTTAGATCGGAAGAGCACACGTCTGAACTCC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGGATGTAAAGAAGTATGTATAGATCGGAAGAGCACACGTCTGAACTCC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCAGATCGGAAGAGCACACGTCTGAACTCCA	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGGTCCCCTTCAACCAGCTGTAGATCGGAAGAGCACACGTCTGAACTCC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGGAAGATCGGAAGAGCACACGTCTGAACTCC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG	7	0.17500000000000002	No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC	7	0.17500000000000002	No Hit
TGTAAACATCCCCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGAATGTAAAGAAGTGTGTATAAGATCGGAAGAGCACACGTCTGAACTC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGAANGTAAAGAAGTATGTATAGATCGGAAGAGCACACGTCTGAACTCC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATGTAAGGAAGTATGTATAGATCGGAAGAGCACACGTCTGAACTCC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC	7	0.17500000000000002	No Hit
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGAATGTAAAGAAGTGTGTATAGATCGGAAGAGCACACGTCTGAACTCC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATGTAAAGAAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGAATGTAAAGAAGTATGTACTAGATCGGAAGAGCACACGTCTGAACTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGGAATGTAAAGAAGTGTGTATAGATCGGAAGAGCACACGTCTGAACTCC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT	5	0.125	No Hit
TTCAAGTAATCCAGGATAGGCAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTGGACTTGGAGTCAGAAGAAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAAAC	5	0.125	No Hit
CTGGACGCGAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAA	5	0.125	TruSeq Adapter, Index 2 (97% over 36bp)
AGGAATGTAAAGAAGTGTGTAAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATGTAAGGAAGTGTGTGAAGATCGGAAGAGCACACGTCTGAACTCC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAGTGTAAAGAAGTATGTATAGATCGGAAGAGCACACGTCTGAACTCC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTGGTCCCCTTCAACCAGCTGTAGATCGGAAGAGCACACGTCTGAACTC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.075	0.0	0.0	0.0	0.0
2	0.075	0.0	0.0	0.0	0.0
3	0.075	0.0	0.0	0.0	0.0
4	0.075	0.0	0.0	0.0	0.0
5	0.075	0.0	0.0	0.0	0.0
6	0.075	0.0	0.0	0.0	0.0
7	0.075	0.0	0.0	0.0	0.0
8	0.075	0.0	0.0	0.0	0.0
9	0.075	0.0	0.0	0.0	0.0
10	0.075	0.0	0.0	0.0	0.0
11	0.15	0.0	0.0	0.0	0.0
12	0.2	0.0	0.0	0.0	0.0
13	0.475	0.0	0.0	0.0	0.0
14	0.95	0.0	0.0	0.0	0.0
15	1.25	0.0	0.0	0.0	0.0
16	1.575	0.0	0.0	0.0	0.0
17	2.1	0.0	0.0	0.0	0.0
18	2.475	0.0	0.0	0.0	0.0
19	3.35	0.0	0.0	0.0	0.0
20	3.85	0.0	0.0	0.0	0.0
21	16.2	0.0	0.0	0.0	0.0
22	24.05	0.0	0.0	0.0	0.0
23	74.05	0.0	0.0	0.0	0.0
24	81.45	0.0	0.0	0.0	0.0
25	82.85	0.0	0.0	0.0	0.0
26	83.175	0.0	0.0	0.0	0.0
27	83.425	0.0	0.0	0.0	0.0
28	83.75	0.0	0.0	0.0	0.0
29	83.975	0.0	0.0	0.0	0.0
30	84.225	0.0	0.0	0.0	0.0
31	85.675	0.0	0.0	0.0	0.0
32	89.075	0.0	0.0	0.0	0.0
33	90.15	0.0	0.0	0.0	0.0
34	90.3	0.0	0.0	0.0	0.0
35	90.525	0.0	0.0	0.0	0.0
36	90.875	0.0	0.0	0.0	0.0
37	91.0	0.0	0.0	0.0	0.0
38	91.2	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGGTAG	20	6.952051E-4	44.000004	2
CATTGGT	20	6.952051E-4	44.000004	2
AGAAGTA	180	0.0	44.000004	11
ATAAGAT	20	6.952051E-4	44.000004	21
ATTGGTG	20	6.952051E-4	44.000004	3
TATAAGA	20	6.952051E-4	44.000004	20
GTATAAG	20	6.952051E-4	44.000004	19
GGTAGTA	20	6.952051E-4	44.000004	4
TGAGGTA	20	6.952051E-4	44.000004	1
TTGGTGG	20	6.952051E-4	44.000004	4
AGGTAGT	20	6.952051E-4	44.000004	3
TGGTGGT	20	6.952051E-4	44.000004	5
GCATTGG	20	6.952051E-4	44.000004	1
TGTATAA	20	6.952051E-4	44.000004	18
TGTGTAA	20	6.952051E-4	44.000004	16
GTAGTAG	20	6.952051E-4	44.000004	5
GTATGTA	190	0.0	44.0	15
AAGTATG	190	0.0	44.0	13
GTAGAAT	25	3.6958358E-5	44.0	18
TGGTTCA	25	3.6958358E-5	44.0	8
>>END_MODULE
Rejected 1043120 READS because READLEN < 1
Read 1043120 spots for SRR17096853.sra
Written 1043120 spots for SRR17096853.sra
Rejected 1043120 READS because READLEN < 1
Read 1043120 spots for SRR17096853.sra
Written 1043120 spots for SRR17096853.sra
Rejected 1043120 READS because READLEN < 1
Read 1043120 spots for SRR17096853.sra
Written 1043120 spots for SRR17096853.sra
Rejected 1043126 READS because READLEN < 1
Read 1043126 spots for SRR17096853.sra
Written 1043126 spots for SRR17096853.sra
Rejected 1043120 READS because READLEN < 1
Read 1043120 spots for SRR17096853.sra
Written 1043120 spots for SRR17096853.sra
Rejected 1043120 READS because READLEN < 1
Read 1043120 spots for SRR17096853.sra
Written 1043120 spots for SRR17096853.sra
Rejected 1043120 READS because READLEN < 1
Read 1043120 spots for SRR17096853.sra
Written 1043120 spots for SRR17096853.sra
Rejected 1043120 READS because READLEN < 1
Read 1043120 spots for SRR17096853.sra
Written 1043120 spots for SRR17096853.sra
SRR ids: ['SRR17096853.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_w6_jg3kk
SRR17096853.sra spots: 8344966
blocks: [[1, 1043120], [1043121, 2086240], [2086241, 3129360], [3129361, 4172480], [4172481, 5215600], [5215601, 6258720], [6258721, 7301840], [7301841, 8344966]]
SRR17096853 file size 1163192
SRR17096853 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR17096853 SRR17096853_1.fastq
Input file:	SRR17096853_1.fastq
trimmed:	SRR17096853-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Wed Nov 26 13:42:03 2025 >> started

Wed Nov 26 13:42:06 2025 >> done (3.036s)
8344966 reads processed; of these:
 176239 ( 2.11%) short reads filtered out after trimming by size control
   2690 ( 0.03%) empty reads filtered out after trimming by size control
8166037 (97.86%) reads available; of these:
  67016 ( 0.82%) trimmed reads available after processing
8099021 (99.18%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  66832	  0.82%
 19	      0	  0.00%
 20	      0	  0.00%
 21	      0	  0.00%
 22	      0	  0.00%
 23	      0	  0.00%
 24	      0	  0.00%
 25	      0	  0.00%
 26	      0	  0.00%
 27	      0	  0.00%
 28	      0	  0.00%
 29	      0	  0.00%
 30	      0	  0.00%
 31	      0	  0.00%
 32	      0	  0.00%
 33	      0	  0.00%
 34	      0	  0.00%
 35	      0	  0.00%
 36	      0	  0.00%
 37	      0	  0.00%
 38	      0	  0.00%
 39	      0	  0.00%
 40	      0	  0.00%
 41	      0	  0.00%
 42	      0	  0.00%
 43	      0	  0.00%
 44	      0	  0.00%
 45	      0	  0.00%
 46	      0	  0.00%
 47	      0	  0.00%
 48	     12	  0.00%
 49	    172	  0.00%
 50	8099021	 99.18%
8166037 reads passed initial QC


criterion=sequence-density
sequence-density=59.81
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=14
prefix-density=0.01
prefix-fanout=1.0
sequence=TGGAATGTAAAGAAGTATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTC


criterion=fanout-score
sequence-density=1.01
sequence-density-rank=15
fanout-score=21.75
fanout-score-rank=1
prefix-density=18.55
prefix-fanout=1.2
sequence=GAAGTGTGTATTAGATC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGGAATGTAAAGAAGTATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTC -o SRR17096853 -
Input file:	STDIN
trimmed:	SRR17096853-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATGTAAAGAAGTATGTATAGATCGGAAGAGCACACGTCTGAACTCCAGTC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Wed Nov 26 13:42:14 2025 >> started

Wed Nov 26 13:42:22 2025 >> done (7.794s)
7893836 reads processed; of these:
   4171 ( 0.05%) short reads filtered out after trimming by size control
4956471 (62.79%) empty reads filtered out after trimming by size control
2933194 (37.16%) reads available; of these:
  11322 ( 0.39%) trimmed reads available after processing
2921872 (99.61%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  38305	  1.31%
 19	      6	  0.00%
 20	     18	  0.00%
 21	     26	  0.00%
 22	    466	  0.02%
 23	     32	  0.00%
 24	     10	  0.00%
 25	      8	  0.00%
 26	      5	  0.00%
 27	      6	  0.00%
 28	      4	  0.00%
 29	      5	  0.00%
 30	     12	  0.00%
 31	      4	  0.00%
 32	      2	  0.00%
 33	      2	  0.00%
 34	      2	  0.00%
 35	      0	  0.00%
 36	      0	  0.00%
 37	      1	  0.00%
 38	      0	  0.00%
 39	      0	  0.00%
 40	      0	  0.00%
 41	      0	  0.00%
 42	      2	  0.00%
 43	      1	  0.00%
 44	     11	  0.00%
 45	    271	  0.01%
 46	   4321	  0.15%
 47	   6107	  0.21%
 48	      6	  0.00%
 49	     54	  0.00%
 50	2883507	 98.31%


criterion=sequence-density
sequence-density=76.79
sequence-density-rank=1
fanout-score=23.22
fanout-score-rank=1
prefix-density=78.80
prefix-fanout=22.6
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC


criterion=fanout-score
sequence-density=76.79
sequence-density-rank=1
fanout-score=23.22
fanout-score-rank=1
prefix-density=78.80
prefix-fanout=22.6
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
                                 Started job on |	Nov 26 13:42:33
                             Started mapping on |	Nov 26 13:42:33
                                    Finished on |	Nov 26 13:42:48
       Mapping speed, Million of reads per hour |	769.29

                          Number of input reads |	3205395
                      Average input read length |	49
                                    UNIQUE READS:
                   Uniquely mapped reads number |	181865
                        Uniquely mapped reads % |	5.67%
                          Average mapped length |	41.95
                       Number of splices: Total |	13765
            Number of splices: Annotated (sjdb) |	9400
                       Number of splices: GT/AG |	13472
                       Number of splices: GC/AG |	73
                       Number of splices: AT/AC |	4
               Number of splices: Non-canonical |	216
                      Mismatch rate per base, % |	1.09%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.31
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.08
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	428923
             % of reads mapped to multiple loci |	13.38%
        Number of reads mapped to too many loci |	21602
             % of reads mapped to too many loci |	0.67%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	80.22%
                     % of reads unmapped: other |	0.05%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	2594607	2594607	2594607
N_multimapping	428923	428923	428923
N_noFeature	47354	48738	177738
N_ambiguous	6689	3921	27
UnstrandedReadsAssigned:127822 PositiveStrandReadsAssigned:129206 NegativeStrandReadsAssigned:4100
Dataset is classified positive stranded
MeadianReadLen=50 20thPercentileLength=50 echo kmer=45
SRR17096853 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 31,715
[index] number of k-mers: 49,915,185
[index] number of equivalence classes: 70,351
[quant] running in single-end mode
[quant] will process file 1: SRR17096853-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,205,395 reads, 74,601 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 785 rounds

  31716 SRR17096853.ke.tsv
  32883 SRR17096853.se.tsv
  64599 total
==> SRR17096853.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	856	804	16956.1
ENSRNOT00000040993.4	1039	940	1877	36047.9
ENSRNOT00000050156.3	1545	1446	3164	39501.4
ENSRNOT00000043693.3	684	585	2028	62583
ENSRNOT00000046201.3	204	105	330	56737.3
ENSRNOT00000046108.3	681	582	1263	39176.4
ENSRNOT00000049683.3	784	685	1434	37792.2
ENSRNOT00000041241.3	348	249	285	20662.8
ENSRNOT00000044582.3	297	198	145	13220.5

==> SRR17096853.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	0
ENSRNOG00000058808	0
ENSRNOG00000061316	0
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	0
SRR17096853 completed mapping pipeline successfully
