Starting /dee2/code/volunteer_pipeline.sh SRR17096856
    current disk space = 421205668
    free memory = 196022808 
SRR17096856 SRAfilesize
a4586227dcafb6834d6c106466fd24bf  SRR17096856.sra
SRR17096856.sra file validated
SRR17096856 is single end
SRR17096856 is conventional basespace
SRR17096856 read1 length is 50 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR17096856_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.97525	32.0	32.0	32.0	32.0	32.0
2	31.1825	32.0	32.0	32.0	32.0	32.0
3	31.23625	32.0	32.0	32.0	32.0	32.0
4	31.284	32.0	32.0	32.0	32.0	32.0
5	31.463	32.0	32.0	32.0	32.0	32.0
6	34.5525	36.0	36.0	36.0	32.0	36.0
7	34.33125	36.0	36.0	36.0	32.0	36.0
8	34.46125	36.0	36.0	36.0	32.0	36.0
9	34.453	36.0	36.0	36.0	32.0	36.0
10	34.72775	36.0	36.0	36.0	32.0	36.0
11	34.80725	36.0	36.0	36.0	32.0	36.0
12	34.79525	36.0	36.0	36.0	32.0	36.0
13	34.80025	36.0	36.0	36.0	32.0	36.0
14	34.633	36.0	36.0	36.0	32.0	36.0
15	34.689	36.0	36.0	36.0	32.0	36.0
16	34.8135	36.0	36.0	36.0	32.0	36.0
17	34.887	36.0	36.0	36.0	32.0	36.0
18	34.92125	36.0	36.0	36.0	36.0	36.0
19	34.92825	36.0	36.0	36.0	36.0	36.0
20	34.812	36.0	36.0	36.0	36.0	36.0
21	34.87825	36.0	36.0	36.0	32.0	36.0
22	34.5745	36.0	36.0	36.0	32.0	36.0
23	34.4675	36.0	36.0	36.0	32.0	36.0
24	34.57	36.0	36.0	36.0	32.0	36.0
25	34.3065	36.0	36.0	36.0	32.0	36.0
26	34.44525	36.0	36.0	36.0	32.0	36.0
27	34.41375	36.0	36.0	36.0	32.0	36.0
28	34.5615	36.0	36.0	36.0	32.0	36.0
29	34.61575	36.0	36.0	36.0	32.0	36.0
30	34.631	36.0	36.0	36.0	32.0	36.0
31	34.19525	36.0	36.0	36.0	32.0	36.0
32	34.49725	36.0	36.0	36.0	32.0	36.0
33	34.2075	36.0	36.0	36.0	32.0	36.0
34	34.3815	36.0	36.0	36.0	32.0	36.0
35	34.5275	36.0	36.0	36.0	32.0	36.0
36	34.39125	36.0	36.0	36.0	32.0	36.0
37	34.11075	36.0	36.0	36.0	32.0	36.0
38	34.16575	36.0	36.0	36.0	32.0	36.0
39	33.9435	36.0	36.0	36.0	32.0	36.0
40	33.79975	36.0	36.0	36.0	32.0	36.0
41	33.95275	36.0	36.0	36.0	32.0	36.0
42	33.96525	36.0	36.0	36.0	32.0	36.0
43	33.855	36.0	36.0	36.0	32.0	36.0
44	34.04325	36.0	36.0	36.0	32.0	36.0
45	33.964	36.0	36.0	36.0	32.0	36.0
46	34.032	36.0	36.0	36.0	32.0	36.0
47	33.90475	36.0	36.0	36.0	32.0	36.0
48	33.80225	36.0	36.0	36.0	27.0	36.0
49	33.7765	36.0	36.0	36.0	32.0	36.0
50	33.78175	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	5.0
23	9.0
24	10.0
25	12.0
26	29.0
27	49.0
28	64.0
29	104.0
30	149.0
31	156.0
32	196.0
33	357.0
34	772.0
35	2086.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.575	17.349999999999998	49.05	14.025000000000002
2	38.925	18.825	18.025	24.224999999999998
3	42.225	24.975	16.650000000000002	16.150000000000002
4	15.275	41.475	23.474999999999998	19.775000000000002
5	16.1	42.25	28.849999999999998	12.8
6	28.03410230692076	14.944834503510531	45.18555667001003	11.835506519558676
7	52.825	16.075	14.674999999999999	16.425
8	18.7	21.675	47.575	12.049999999999999
9	24.6	40.2	22.05	13.15
10	31.225	38.375	19.25	11.15
11	24.875	36.525	23.65	14.95
12	34.050000000000004	21.975	26.724999999999998	17.25
13	13.025	44.425	16.825000000000003	25.724999999999998
14	13.875000000000002	50.425	23.45	12.25
15	51.349999999999994	16.05	21.65	10.95
16	15.525	23.825	47.15	13.5
17	29.775000000000002	39.275	18.125	12.825000000000001
18	26.05	21.725	40.375	11.85
19	38.3	22.650000000000002	27.400000000000002	11.65
20	20.375	28.499999999999996	41.4	9.725
21	29.075	39.925	19.675	11.325000000000001
22	22.05	33.825	28.825	15.299999999999999
23	20.025000000000002	50.575	12.625	16.775000000000002
24	39.725	23.150000000000002	25.2	11.924999999999999
25	21.525	35.925000000000004	21.275	21.275
26	21.15	20.474999999999998	28.225	30.15
27	21.95	20.599999999999998	22.55	34.9
28	36.1	20.325	15.55	28.025
29	46.85	22.625	14.374999999999998	16.150000000000002
30	31.25	37.3	8.774999999999999	22.675
31	20.275000000000002	49.6	10.2	19.925
32	39.45	27.0	15.65	17.9
33	23.125	41.85	11.35	23.674999999999997
34	35.699999999999996	29.15	13.925	21.224999999999998
35	22.35	26.474999999999998	12.3	38.875
36	17.549999999999997	51.725	10.9	19.825
37	19.225	31.025000000000002	10.674999999999999	39.074999999999996
38	27.224999999999998	38.475	11.65	22.650000000000002
39	23.974999999999998	23.549999999999997	19.35	33.125
40	39.225	19.3	17.075000000000003	24.4
41	21.425	22.925	38.550000000000004	17.1
42	22.925	25.7	20.8	30.575000000000003
43	23.65	25.525	31.175000000000004	19.650000000000002
44	33.45	32.1	16.375	18.075
45	19.875	45.324999999999996	11.05	23.75
46	17.0	45.95	14.975	22.075
47	11.924999999999999	30.2	15.049999999999999	42.825
48	13.4	24.325	30.575000000000003	31.7
49	13.975000000000001	27.625	16.1	42.3
50	23.025000000000002	19.05	13.55	44.375
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	1.0
19	1.5
20	2.0
21	3.0
22	4.0
23	3.5
24	3.0
25	4.0
26	5.0
27	6.0
28	7.0
29	9.5
30	12.0
31	17.0
32	22.0
33	32.0
34	42.0
35	62.5
36	83.0
37	96.0
38	109.0
39	170.5
40	232.0
41	389.5
42	547.0
43	528.5
44	510.0
45	460.0
46	410.0
47	364.0
48	318.0
49	402.0
50	486.0
51	500.5
52	515.0
53	389.5
54	264.0
55	208.5
56	153.0
57	150.5
58	148.0
59	101.5
60	55.0
61	42.0
62	29.0
63	25.0
64	21.0
65	16.0
66	11.0
67	8.5
68	6.0
69	3.5
70	1.0
71	1.0
72	1.0
73	1.0
74	1.0
75	1.0
76	1.0
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.3
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.35
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.56639566395664	49.575
2	4.290876242095754	4.75
3	1.9873532068654018	3.3000000000000003
4	0.7678410117434508	1.7000000000000002
5	0.6775067750677507	1.875
6	0.5871725383920506	1.95
7	0.27100271002710025	1.05
8	0.22583559168925021	1.0
9	0.045167118337850046	0.22499999999999998
>10	1.2646793134598013	14.2
>50	0.22583559168925021	9.125
>100	0.09033423667570009	11.25
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TGGAATGTAAAGAAGTATGTATAGATCGGAAGAGCACACGTCTGAACTCC	326	8.15	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATGTAAAGAAGTATGTAGATCGGAAGAGCACACGTCTGAACTCCAG	124	3.1	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAAAC	90	2.25	No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG	83	2.075	No Hit
TGGAATGTAAAGAAGTGTGTATAGATCGGAAGAGCACACGTCTGAACTCC	66	1.6500000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC	63	1.575	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG	63	1.575	No Hit
TGCACTGATGAAACTAGAAATAGGAAGTGCCGTCCGAAACGACAACTGAC	46	1.15	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT	39	0.975	No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC	37	0.9249999999999999	No Hit
TGGAATGTAAAGAAGTATGTACAGATCGGAAGAGCACACGTCTGAACTCC	35	0.8750000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG	34	0.8500000000000001	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC	31	0.775	No Hit
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC	31	0.775	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATGTAAAGAAGTATGTAAGATCGGAAGAGCACACGTCTGAACTCCA	29	0.7250000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGCTGTGATGACTATCTTAGGACACCTTTGGATTAACCGTGAAATAAAA	28	0.7000000000000001	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAGATCGGAAGAGCACA	20	0.5	No Hit
CTGGACGCGAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAT	19	0.475	TruSeq Adapter, Index 9 (97% over 36bp)
ACTGGACTTGGAGTCAGAAGGCAGATCGGAAGAGCACACGTCTGAACTCC	18	0.44999999999999996	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATGTAAAGAAGTATGTATTAGATCGGAAGAGCACACGTCTGAACTC	17	0.42500000000000004	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGAATGTAAAGAAGTGTGTAAGATCGGAAGAGCACACGTCTGAACTCCA	16	0.4	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTCTGTGATGAATCAACTAGCTCACTATGACTGCTAATGAAAACACTGG	15	0.375	No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAAAA	14	0.35000000000000003	No Hit
TGGAATGTAAAGAAGTATGTAAAGATCGGAAGAGCACACGTCTGAACTCC	14	0.35000000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG	14	0.35000000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCG	13	0.325	No Hit
TGGAATGTAAAGAAGTGTGTACAGATCGGAAGAGCACACGTCTGAACTCC	13	0.325	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT	12	0.3	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTGTGATGAATCAACTAGCTCACTATGACTGCTAATGAAAACACTGGA	11	0.27499999999999997	No Hit
AGGAATGTAAAGAAGTATGTATAGATCGGAAGAGCACACGTCTGAACTCC	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGACGCGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATG	11	0.27499999999999997	TruSeq Adapter, Index 12 (97% over 37bp)
TAGCTTATCAGACTGATGTTGATAGATCGGAAGAGCACACGTCTGAACTC	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTAAATGATGAGATTTCCATTGGTCCGTGTTTCTGAACTCCATGATTTTT	10	0.25	No Hit
ATACATGATGATCTCACTCCAACTTGAACTCTCTCACTGATTACTTGATG	10	0.25	No Hit
TGGAATGTAAAGAAGTATGCAGATCGGAAGAGCACACGTCTGAACTCCAG	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCAC	9	0.22499999999999998	No Hit
TGGAATGTAAAGAAGTGTGTAGATCGGAAGAGCACACGTCTGAACTCCAG	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACTGTGATGATAACATAGTTCAGCAGACGTGACTCTGGTGAACAATCAT	8	0.2	No Hit
AAGAAAGTATGCAAGAACTAGATCGGAAGAGCACACGTCTGAACTCCAGT	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC	8	0.2	No Hit
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATGTAAAGAAGTGTGTAAAGATCGGAAGAGCACACGTCTGAACTCC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATGTAAAGAAGTATGTGTAGATCGGAAGAGCACACGTCTGAACTCC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTGGACGCGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAT	7	0.17500000000000002	TruSeq Adapter, Index 9 (97% over 36bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC	6	0.15	No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT	6	0.15	No Hit
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTAGATCGGAAG	6	0.15	No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACGT	6	0.15	No Hit
TGGAATGTAAGGAAGTGTGTGGAGATCGGAAGAGCACACGTCTGAACTCC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATGTAAAGAAGTATGTAGAGATCGGAAGAGCACACGTCTGAACTCC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACCCGTAGATCCGATCTTGTAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCTCCGTCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGG	6	0.15	TruSeq Adapter, Index 12 (97% over 37bp)
GCATTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATCGG	6	0.15	No Hit
TGAAGTGATGATTCACAATCATGTCTCTTCTCTGAAGAATTCTTGGAGAT	6	0.15	No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG	6	0.15	No Hit
TGTAAACATCCTCGACTGGAAGCAGATCGGAAGAGCACACGTCTGAACTC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC	5	0.125	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT	5	0.125	No Hit
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGAGATCGGAAGAGCACACGTCTGAACTCCAG	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTAGATCGGAAGAGCACACGTCTGAACT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCCAGATCGGAAGAGCACACG	5	0.125	No Hit
TTCACAGTGGCTAAGTTCTGCAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTTCTGTGATGAGACTCTGGAATGTGCGCTGGGCACAGCGCCCGAGTGAG	5	0.125	No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTCACCAGATCGGAAGA	5	0.125	No Hit
TGGAAGACTAGTGATTTTGTTGTTAGATCGGAAGAGCACACGTCTGAACT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAAGATCGGAAGAGCACA	5	0.125	No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.025	0.0	0.0	0.0	0.0
8	0.05	0.0	0.0	0.0	0.0
9	0.05	0.0	0.0	0.0	0.0
10	0.175	0.0	0.0	0.0	0.0
11	0.375	0.0	0.0	0.0	0.0
12	1.125	0.0	0.0	0.0	0.0
13	2.125	0.0	0.0	0.0	0.0
14	3.825	0.0	0.0	0.0	0.0
15	4.5	0.0	0.0	0.0	0.0
16	5.4	0.0	0.0	0.0	0.0
17	6.75	0.0	0.0	0.0	0.0
18	8.0	0.0	0.0	0.0	0.0
19	9.55	0.0	0.0	0.0	0.0
20	10.9	0.0	0.0	0.0	0.0
21	17.1	0.0	0.0	0.0	0.0
22	20.9	0.0	0.0	0.0	0.0
23	40.325	0.0	0.0	0.0	0.0
24	45.45	0.0	0.0	0.0	0.0
25	47.725	0.0	0.0	0.0	0.0
26	49.1	0.0	0.0	0.0	0.0
27	49.85	0.0	0.0	0.0	0.0
28	50.975	0.0	0.0	0.0	0.0
29	52.65	0.0	0.0	0.0	0.0
30	53.9	0.0	0.0	0.0	0.0
31	55.5	0.0	0.0	0.0	0.0
32	58.25	0.0	0.0	0.0	0.0
33	61.425	0.0	0.0	0.0	0.0
34	63.7	0.0	0.0	0.0	0.0
35	65.7	0.0	0.0	0.0	0.0
36	66.8	0.0	0.0	0.0	0.0
37	67.875	0.0	0.0	0.0	0.0
38	68.525	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGTAG	20	6.952051E-4	44.000004	16
TAGCTTA	20	6.952051E-4	44.000004	1
CTGATGT	20	6.952051E-4	44.000004	13
GGTGGTT	45	2.728484E-10	44.000004	6
ATGTAGA	20	6.952051E-4	44.000004	17
AATGTAA	80	0.0	44.000004	4
CTCGCCT	20	6.952051E-4	44.000004	26
GTATGTA	60	0.0	44.0	15
CATTGGT	35	1.02281774E-7	44.0	2
AGCTTAT	25	3.6958358E-5	44.0	2
AGAAGTA	70	0.0	44.0	11
TATGTAT	35	1.02281774E-7	44.0	16
ATTGGTG	35	1.02281774E-7	44.0	3
GAAGTAT	70	0.0	44.0	12
GTATAGA	35	1.02281774E-7	44.0	19
AGTATGT	60	0.0	44.0	14
ATCAGAC	25	3.6958358E-5	44.0	7
TCTCGCC	35	1.02281774E-7	44.0	25
TTCTCGC	35	1.02281774E-7	44.0	24
TCAGACT	25	3.6958358E-5	44.0	8
>>END_MODULE
Rejected 911561 READS because READLEN < 1
Read 911561 spots for SRR17096856.sra
Written 911561 spots for SRR17096856.sra
Rejected 911561 READS because READLEN < 1
Read 911561 spots for SRR17096856.sra
Written 911561 spots for SRR17096856.sra
Rejected 911564 READS because READLEN < 1
Read 911564 spots for SRR17096856.sra
Written 911564 spots for SRR17096856.sra
Rejected 911561 READS because READLEN < 1
Read 911561 spots for SRR17096856.sra
Written 911561 spots for SRR17096856.sra
Rejected 911561 READS because READLEN < 1
Read 911561 spots for SRR17096856.sra
Written 911561 spots for SRR17096856.sra
Rejected 911561 READS because READLEN < 1
Read 911561 spots for SRR17096856.sra
Written 911561 spots for SRR17096856.sra
Rejected 911561 READS because READLEN < 1
Read 911561 spots for SRR17096856.sra
Written 911561 spots for SRR17096856.sra
Rejected 911561 READS because READLEN < 1
Read 911561 spots for SRR17096856.sra
Written 911561 spots for SRR17096856.sra
SRR ids: ['SRR17096856.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_uetgixy1
SRR17096856.sra spots: 7292491
blocks: [[1, 911561], [911562, 1823122], [1823123, 2734683], [2734684, 3646244], [3646245, 4557805], [4557806, 5469366], [5469367, 6380927], [6380928, 7292491]]
SRR17096856 file size 1016216
SRR17096856 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR17096856 SRR17096856_1.fastq
Input file:	SRR17096856_1.fastq
trimmed:	SRR17096856-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Wed Nov 26 13:43:01 2025 >> started

Wed Nov 26 13:43:04 2025 >> done (3.358s)
7292491 reads processed; of these:
 633277 ( 8.68%) short reads filtered out after trimming by size control
   1556 ( 0.02%) empty reads filtered out after trimming by size control
6657658 (91.29%) reads available; of these:
  96402 ( 1.45%) trimmed reads available after processing
6561256 (98.55%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  96247	  1.45%
 19	      0	  0.00%
 20	      0	  0.00%
 21	      0	  0.00%
 22	      0	  0.00%
 23	      0	  0.00%
 24	      0	  0.00%
 25	      0	  0.00%
 26	      0	  0.00%
 27	      0	  0.00%
 28	      0	  0.00%
 29	      0	  0.00%
 30	      0	  0.00%
 31	      0	  0.00%
 32	      0	  0.00%
 33	      0	  0.00%
 34	      0	  0.00%
 35	      0	  0.00%
 36	      0	  0.00%
 37	      0	  0.00%
 38	      0	  0.00%
 39	      0	  0.00%
 40	      0	  0.00%
 41	      0	  0.00%
 42	      0	  0.00%
 43	      0	  0.00%
 44	      0	  0.00%
 45	      0	  0.00%
 46	      0	  0.00%
 47	      0	  0.00%
 48	      8	  0.00%
 49	    147	  0.00%
 50	6561256	 98.55%
6657658 reads passed initial QC


criterion=sequence-density
sequence-density=64.66
sequence-density-rank=1
fanout-score=19.45
fanout-score-rank=1
prefix-density=67.97
prefix-fanout=18.5
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGC


criterion=fanout-score
sequence-density=64.66
sequence-density-rank=1
fanout-score=19.45
fanout-score-rank=1
prefix-density=67.97
prefix-fanout=18.5
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGC -o SRR17096856 -
Input file:	STDIN
trimmed:	SRR17096856-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Wed Nov 26 13:43:13 2025 >> started

Wed Nov 26 13:43:18 2025 >> done (5.145s)
6452807 reads processed; of these:
   3880 ( 0.06%) short reads filtered out after trimming by size control
      3 ( 0.00%) empty reads filtered out after trimming by size control
6448924 (99.94%) reads available; of these:
5211886 (80.82%) trimmed reads available after processing
1237038 (19.18%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  93006	  1.44%
 19	 105889	  1.64%
 20	 428554	  6.65%
 21	 296704	  4.60%
 22	1400817	 21.72%
 23	 353744	  5.49%
 24	 151994	  2.36%
 25	  66372	  1.03%
 26	  64362	  1.00%
 27	  82174	  1.27%
 28	 142045	  2.20%
 29	  95621	  1.48%
 30	  95296	  1.48%
 31	 200596	  3.11%
 32	 267475	  4.15%
 33	 152868	  2.37%
 34	 140048	  2.17%
 35	  96530	  1.50%
 36	  60733	  0.94%
 37	  48032	  0.74%
 38	  66516	  1.03%
 39	  73413	  1.14%
 40	  51232	  0.79%
 41	  62344	  0.97%
 42	  42621	  0.66%
 43	 128509	  1.99%
 44	 286779	  4.45%
 45	 144371	  2.24%
 46	  50101	  0.78%
 47	  53176	  0.82%
 48	      1	  0.00%
 49	     29	  0.00%
 50	1146972	 17.79%


criterion=sequence-density
sequence-density=20.99
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=12
prefix-density=0.00
prefix-fanout=1.0
sequence=TGGAATGTAAAGAAGTATGTATT


criterion=fanout-score
sequence-density=0.27
sequence-density-rank=25
fanout-score=69.52
fanout-score-rank=1
prefix-density=18.05
prefix-fanout=1.0
sequence=AAGAAGTATGTGT
                                 Started job on |	Nov 26 13:43:29
                             Started mapping on |	Nov 26 13:43:29
                                    Finished on |	Nov 26 13:43:57
       Mapping speed, Million of reads per hour |	855.49

                          Number of input reads |	6653775
                      Average input read length |	32
                                    UNIQUE READS:
                   Uniquely mapped reads number |	4011131
                        Uniquely mapped reads % |	60.28%
                          Average mapped length |	30.59
                       Number of splices: Total |	69000
            Number of splices: Annotated (sjdb) |	63761
                       Number of splices: GT/AG |	67713
                       Number of splices: GC/AG |	503
                       Number of splices: AT/AC |	73
               Number of splices: Non-canonical |	711
                      Mismatch rate per base, % |	0.29%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.22
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.12
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1711876
             % of reads mapped to multiple loci |	25.73%
        Number of reads mapped to too many loci |	664650
             % of reads mapped to too many loci |	9.99%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	3.82%
                     % of reads unmapped: other |	0.18%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	930768	930768	930768
N_multimapping	1711876	1711876	1711876
N_noFeature	465238	829964	3619050
N_ambiguous	82789	55353	94
UnstrandedReadsAssigned:3463104 PositiveStrandReadsAssigned:3125814 NegativeStrandReadsAssigned:391987
Dataset is classified positive stranded
MeadianReadLen=29 20thPercentileLength=22 echo kmer=19
SRR17096856 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 31,715
[index] number of k-mers: 49,362,984
[index] number of equivalence classes: 166,181
[quant] running in single-end mode
[quant] will process file 1: SRR17096856-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 6,653,775 reads, 792,103 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 875 rounds

  31716 SRR17096856.ke.tsv
  32883 SRR17096856.se.tsv
  64599 total
==> SRR17096856.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	856	10421	21598.2
ENSRNOT00000040993.4	1039	940	11051	20857.2
ENSRNOT00000050156.3	1545	1446	66822	81984.7
ENSRNOT00000043693.3	684	585	32985	100033
ENSRNOT00000046201.3	204	105	1541	26037.3
ENSRNOT00000046108.3	681	582	11436	34860.4
ENSRNOT00000049683.3	784	685	14889	38561.8
ENSRNOT00000041241.3	348	249	1280	9119.95
ENSRNOT00000044582.3	297	198	1390	12454.6

==> SRR17096856.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	0
ENSRNOG00000058808	2
ENSRNOG00000061316	17
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	0
SRR17096856 completed mapping pipeline successfully
