Starting /dee2/code/volunteer_pipeline.sh SRR26281818
    current disk space = 712540860
    free memory = 198630348 
SRR26281818 SRAfilesize
eca7e6cda765746f3a3f20e5a62657e2  SRR26281818.sra
SRR26281818.sra file validated
SRR26281818 is paired end
SRR26281818 is conventional basespace
SRR26281818 read1 length is 39-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR26281818_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	39-151
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.264	37.0	37.0	37.0	37.0	37.0
2	36.112	37.0	37.0	37.0	37.0	37.0
3	36.245	37.0	37.0	37.0	37.0	37.0
4	36.351	37.0	37.0	37.0	37.0	37.0
5	36.387	37.0	37.0	37.0	37.0	37.0
6	36.4385	37.0	37.0	37.0	37.0	37.0
7	36.2475	37.0	37.0	37.0	37.0	37.0
8	36.476	37.0	37.0	37.0	37.0	37.0
9	36.4005	37.0	37.0	37.0	37.0	37.0
10-14	36.4149	37.0	37.0	37.0	37.0	37.0
15-19	36.3705	37.0	37.0	37.0	37.0	37.0
20-24	36.3721	37.0	37.0	37.0	37.0	37.0
25-29	36.339299999999994	37.0	37.0	37.0	37.0	37.0
30-34	36.3216	37.0	37.0	37.0	37.0	37.0
35-39	36.2542	37.0	37.0	37.0	37.0	37.0
40-44	36.25842921460731	37.0	37.0	37.0	37.0	37.0
45-49	36.18579289644822	37.0	37.0	37.0	37.0	37.0
50-54	36.229314657328665	37.0	37.0	37.0	37.0	37.0
55-59	36.165044493725475	37.0	37.0	37.0	37.0	37.0
60-64	36.13702101488159	37.0	37.0	37.0	37.0	37.0
65-69	36.080513362521145	37.0	37.0	37.0	37.0	37.0
70-74	36.093953050868734	37.0	37.0	37.0	37.0	37.0
75-79	36.091547171204326	37.0	37.0	37.0	37.0	37.0
80-84	36.1142171407583	37.0	37.0	37.0	37.0	37.0
85-89	35.97474407221741	37.0	37.0	37.0	37.0	37.0
90-94	35.9525033051137	37.0	37.0	37.0	37.0	37.0
95-99	36.02059600923592	37.0	37.0	37.0	37.0	37.0
100-104	35.93724268174374	37.0	37.0	37.0	37.0	37.0
105-109	35.90445123196896	37.0	37.0	37.0	37.0	37.0
110-114	35.929579296612914	37.0	37.0	37.0	37.0	37.0
115-119	35.915954863618516	37.0	37.0	37.0	37.0	37.0
120-124	35.92331996322215	37.0	37.0	37.0	37.0	37.0
125-129	35.78668039529302	37.0	37.0	37.0	37.0	37.0
130-134	35.7548014066365	37.0	37.0	37.0	37.0	37.0
135-139	35.63391892299889	37.0	37.0	37.0	37.0	37.0
140-144	35.55701335643289	37.0	37.0	37.0	37.0	37.0
145-149	35.52406527258686	37.0	37.0	37.0	37.0	37.0
150-151	35.888100933617125	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
26	4.0
27	8.0
28	13.0
29	35.0
30	34.0
31	75.0
32	76.0
33	104.0
34	174.0
35	383.0
36	2470.0
37	624.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.7	22.650000000000002	13.350000000000001	30.3
2	29.719579369053577	20.0050075112669	27.616424636955433	22.658988482724084
3	22.2	26.875	27.85	23.075000000000003
4	19.3	34.375	24.425	21.9
5	22.85	35.575	22.925	18.65
6	17.25	39.025	22.6	21.125
7	17.9	25.85	35.525	20.724999999999998
8	24.625	24.3	25.35	25.724999999999998
9	22.375	23.025000000000002	28.625	25.974999999999998
10-14	22.305	27.544999999999998	27.33	22.82
15-19	22.3	26.86	27.82	23.02
20-24	23.200000000000003	27.965	26.155	22.68
25-29	22.53	28.17	26.07	23.23
30-34	23.150000000000002	28.785	26.005	22.06
35-39	22.1	27.845	27.005000000000003	23.05
40-44	22.821410705352676	27.51375687843922	28.25912956478239	21.405702851425712
45-49	21.970985492746372	27.348674337168582	27.94897448724362	22.73136568284142
50-54	22.75137568784392	28.954477238619308	26.163081540770385	22.131065532766385
55-59	23.167742258242033	26.84976737205463	27.525138826354496	22.457351543348842
60-64	22.02252816020025	28.640801001251564	27.274092615769714	22.062578222778473
65-69	22.49096748293858	26.23444399839422	27.549177037334406	23.7254114813328
70-74	23.61201993255147	29.304877434942366	25.383802285196555	21.69930034730961
75-79	22.553427979588744	28.818269085030064	25.534279795887432	23.09402313949376
80-84	24.608739837398375	27.35772357723577	27.754065040650406	20.279471544715445
85-89	22.436292642827784	27.72297575010275	27.815454171804355	22.025277435265107
90-94	21.73256237953847	28.306506224930978	28.37943428660728	21.58149710892327
95-99	22.673398883869254	28.86526707414297	26.80839755514217	21.652936486845604
100-104	22.24896084007876	28.64252898709254	27.2423977247867	21.866112448042006
105-109	22.487444275153774	28.525478246148637	26.42627391230743	22.56080356639016
110-114	21.99766355140187	29.135514018691588	24.80140186915888	24.065420560747665
115-119	21.793547603135064	27.662676954857524	26.00400996415335	24.53976547785406
120-124	22.498090145148968	29.246243952126306	25.00636618283677	23.249299719887954
125-129	22.98321602598809	27.625879805089333	25.96101786681104	23.429886302111534
130-134	21.621235906950194	28.550021407164266	27.03011274439846	22.798629941487082
135-139	21.482590789966306	28.566080119805314	25.915387495320104	24.035941594908277
140-144	22.89004574854078	28.84524372929484	26.313298627543773	21.951411894620605
145-149	22.29368526561978	29.802873371199468	25.258937520882057	22.644503842298697
150-151	20.49725354148598	11.332755131540907	35.76178086152067	32.40821046545244
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.5
26	8.5
27	15.0
28	15.5
29	13.5
30	10.5
31	22.5
32	40.0
33	45.5
34	49.5
35	68.0
36	90.0
37	114.0
38	122.5
39	126.0
40	157.5
41	182.0
42	168.0
43	183.5
44	270.5
45	288.5
46	263.5
47	236.0
48	201.0
49	205.0
50	195.5
51	187.5
52	174.0
53	124.5
54	92.5
55	68.5
56	44.0
57	59.5
58	70.5
59	41.5
60	28.0
61	24.5
62	7.5
63	6.5
64	8.0
65	6.0
66	1.5
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.15
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35-39	2.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	1.0
60-64	3.0
65-69	16.0
70-74	11.0
75-79	22.0
80-84	30.0
85-89	56.0
90-94	55.0
95-99	109.0
100-104	94.0
105-109	137.0
110-114	117.0
115-119	144.0
120-124	163.0
125-129	187.0
130-134	128.0
135-139	145.0
140-144	127.0
145-149	371.0
150-152	2082.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	38.704930095658575	13.15
2	20.60338484179544	14.000000000000002
3	13.980868285504048	14.249999999999998
4	8.682855040470933	11.799999999999999
5	5.003679175864606	8.5
6	3.6055923473142015	7.35
7	2.57542310522443	6.125
8	2.35467255334805	6.4
9	1.3245033112582782	4.05
>10	3.164091243561442	14.374999999999998
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCTTCTG	31	0.775	No Hit
CCTCCCAACACTGACCCTTGGCTTCTTCGTTCTAAAAGCCCTGTGAGAAA	25	0.625	No Hit
CGTCAAAGTAGTGAAGTGGATTCACAACAAGCCCCCACCTCCCAACACTG	20	0.5	No Hit
GAAAAACCGGCTAAGTTCATTTGACCTTTCCCCTGTCAATCATGAAGACC	18	0.44999999999999996	No Hit
TCTCGATGTCATCCATTTAAGTCAGCATTTGAATGACATATTGTGTATTG	18	0.44999999999999996	No Hit
CGCACACTGCTCCAGCTACTGTGGTTCTTGTGTCAACTGATGTTAATTTT	18	0.44999999999999996	No Hit
GGGAGACACTTCACAGAAGCAGTCAAAAAAAAGAGAACCTCAGTTCCCAG	16	0.4	No Hit
CCAAAATAACGATGCTAAGCCGTGGCTTTGGAAATTCTCTGGTTGCTTTT	15	0.375	No Hit
GGATATTCCAAGCTGATTGAGCTACTAGAAGCCGTGCCTCATGTGTTGCA	15	0.375	No Hit
GAAACAATGTCTATTCTTCAGGATGCTCCTGCCTGTTGTTTGCCTCTCTT	15	0.375	No Hit
CCCCGGGCTACTGATTAGCAAGGATGCAGTGGAAAGCCCAGCAAAAAAGC	15	0.375	No Hit
GGCTACAGTAGTGTGGAGGAGCTACTGGGAGCCATCCCACAGGTGGTCTG	15	0.375	No Hit
CATCACCATTTTGGAAAGCAGTGCCGGGTTTCAGACTATGGATATTCCAA	14	0.35000000000000003	No Hit
GAAAAAACAGAAATTCCAATACCAGTAAAGAAGGGGAACCTGTCCTGTAA	14	0.35000000000000003	No Hit
CCTATACTAAATATGTAGGAGAAACACTGCAGCCCAAAACTTATGGCTAC	13	0.325	No Hit
GTTCATTTGACCTTTCCCCTGTCAATCATGAAGACCAGCCTGCAGTCAGC	13	0.325	No Hit
GGAAAGCCCAGCAAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGCCA	13	0.325	No Hit
CTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCTTCTGCTCCTCC	13	0.325	No Hit
CGCAAAGAATGCTGCTGATGATAAACTCCGTCAGAGCCTGCGAAGGTTTG	12	0.3	No Hit
ATCCATTTAAGTCAGCATTTGAATGACATATTGTGTATTGAGAGGTAATG	12	0.3	No Hit
GAGCCTGCGAAGGTTTGCAAATACGCACACTGCTCCAGCTACTGTGGTTC	12	0.3	No Hit
GCAATGTGGACTACAGGCTGTCCCGGAAGGAGCTACAGCAGCTCCTCCAA	12	0.3	No Hit
CGGGTTTCAGACTATGGATATTCCAAGCTGATTGAGCTACTAGAAGCCGT	12	0.3	No Hit
GTCTAGGCAAAGCTGCTTCTAAATTTGGGTCACCAGAAGTTGCAGTCACT	12	0.3	No Hit
CCTGTGACCAAGCTCTAACTTCCAGTTTGAACACAGAATTAAATTAGGAT	12	0.3	No Hit
AGGAGCTACTGGGAGCCATCCCACAGGTGGTCTGGATAAAAGGACATGGT	12	0.3	No Hit
GCCTCCATCATTTGTTTAAGCAAATGTATTTGTGGTTGTTCAAGAAGCCA	12	0.3	No Hit
CGAGCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCCCATTTTACCA	12	0.3	No Hit
AGAAGGGGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCT	11	0.27499999999999997	No Hit
GCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCCCATTTTACCATGG	11	0.27499999999999997	No Hit
CGGAATGTAAGCGGAGAAGGAGCTGGTGGCTGAGGGTGGAAGAATGAAAG	11	0.27499999999999997	No Hit
GTTTCATGACTTTGACTGAGCTACTGAAGAGTCTGCCTTCTTTAGTAGAG	11	0.27499999999999997	No Hit
AGGTGAAGCGCTTTACTCAGGATTTACTGAAACTTCTCAAGTCCCAGGCC	10	0.25	No Hit
GTCACACACTGGGGCATGATTGAGTGGCAAGAGAGCCATTTTCCGGTTGA	10	0.25	No Hit
AGAAATTCCAATACCAGTAAAGAAGGGGAACCTGTCCTGTAACTCCTCAC	10	0.25	No Hit
CTGGGAAGCTCTCAATGTGAAGATGTGTGTGTCCATGTTAGTTCATCTTG	10	0.25	No Hit
GGAGCTACTGGGAGCCATCCCACAGGTGGTCTGGATAAAAGGACATGGTC	10	0.25	No Hit
CCATGTTAGTTCATCTTGACGTCTTTAATAGTGTCTAGCTTTACTGTTAC	10	0.25	No Hit
CTGGGAGCCATCCCACAGGTGGTCTGGATAAAAGGACATGGTCATAAGAG	10	0.25	No Hit
CTGCCTTGTGGAATGGCCTTCTCTTCTGGGTGCCTGAGGTATCCACTTAT	10	0.25	No Hit
GCGCTTTACTCAGGATTTACTGAAACTTCTCAAGTCCCAGGCCAGCAAGC	10	0.25	No Hit
GTGGAAAGCCCAGCAAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGC	10	0.25	No Hit
GCTCGAACAAGGACTTCAGCGAACTTGTATTTGATCCAGACTCTTACAAG	10	0.25	No Hit
GCCGTGCCTCATGTGTTGCAGATTCTTGGAATGGGCTCCAAGCGTCTACT	9	0.22499999999999998	No Hit
GTTTTTTAGAGGACACAGAGAAGCAGAGTTTATCTGTGTGTGTGACATCA	9	0.22499999999999998	No Hit
ATTTGCTTGTCCCAACAAGATGATGACATGGTCATTTGTATCCGAAAAAG	9	0.22499999999999998	No Hit
AGTGGATTCACAACAAGCCCCCACCTCCCAACACTGACCCTTGGCTTCTT	9	0.22499999999999998	No Hit
AGAAGGAGCTGGTGGCTGAGGGTGGAAGAATGAAAGAAAAGAAAGATGAA	9	0.22499999999999998	No Hit
TGTGAATTGTGGTGGTTGGGAAGAAATAAGCAGAGATCGTTCAGCAGGAA	9	0.22499999999999998	No Hit
CTCTGAAGCCTTGTTACATCATGCTAACCAGCTGATCAGATTTGAAGAGT	9	0.22499999999999998	No Hit
AAACTTTAAAGGTTTACAGGAGCTGTGCCGAATGGAACCAAAGTCTGGCA	9	0.22499999999999998	No Hit
AGATACTGGTGTCACTCTCCACAGGAGCTGCCAACAAGTCACTCTCTTTA	9	0.22499999999999998	No Hit
CAGATTTGAAGAGTTCATTTCCGATCTACCCCCCAGGTTACCACTGAAGA	9	0.22499999999999998	No Hit
CTCAAGTTCATAGTCTCCTGCAGACCCACGAGGGCACTGTGCCTTTGCTG	9	0.22499999999999998	No Hit
GTTTTATTTTTAAACCAAGAAGAAAAAACAGAAATTCCAATACCAGTAAA	9	0.22499999999999998	No Hit
TCGCACCTCAAGTTCATAGTCTCCTGCAGACCCACGAGGGCACTGTGCCT	9	0.22499999999999998	No Hit
GTTCAGTCTCAGCTTTGACACAGAAGCTCTGCCATGTTGTAAAGGTTGCT	9	0.22499999999999998	No Hit
GGCAAAGCTGCTTCTAAATTTGGGTCACCAGAAGTTGCAGTCACTGGACA	9	0.22499999999999998	No Hit
CTCCCTGGGATCTTCGCCGCCCGAGCTGGACATCATGCCACAGGAAGAGT	9	0.22499999999999998	No Hit
AAAACTTATGGCTACAGTAGTGTGGAGGAGCTACTGGGAGCCATCCCACA	9	0.22499999999999998	No Hit
CAAAACTTATGGCTACAGTAGTGTGGAGGAGCTACTGGGAGCCATCCCAC	9	0.22499999999999998	No Hit
AGATTCCTTGCCACACTCTACTCTATGTTTATAACCTGCCGGCAAATAAA	8	0.2	No Hit
TGTCAGTTCAGTCTCAGCTTTGACACAGAAGCTCTGCCATGTTGTAAAGG	8	0.2	No Hit
GCTACCCATCTGCATTCAGCCAGCTGATCGCCTCAAGGCAAGTCAGTCCT	8	0.2	No Hit
TTTTAAACCAAGAAGAAAAAACAGAAATTCCAATACCAGTAAAGAAGGGG	8	0.2	No Hit
AAAGCTGCTTCTAAATTTGGGTCACCAGAAGTTGCAGTCACTGGACAGGT	8	0.2	No Hit
GCAGGTCATTGTGAGCGATTTCTCACAGGCTTATCATTGGTGTTTCTCAA	8	0.2	No Hit
CAGTAAAGAAGGGGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCA	8	0.2	No Hit
GTTCATTTCCGATCTACCCCCCAGGTTACCACTGAAGATTCCTTGCCACA	8	0.2	No Hit
CTATTCTTCAGGATGCTCCTGCCTGTTGTTTGCCTCTCTTTAAATTTATA	8	0.2	No Hit
CACAACTATTTGCTTGTCCCAACAAGATGATGACATGGTCATTTGTATCC	8	0.2	No Hit
GTCTAAACAAGCCAGCAAGAAATAGTATAATTGATGCTGCTAAAATCTGG	8	0.2	No Hit
AAAAGAAGTATGGACACAAGTTAAATGTGTCTGATTTATATAAATTAACA	8	0.2	No Hit
TCTCAATGTGAAGATGTGTGTGTCCATGTTAGTTCATCTTGACGTCTTTA	8	0.2	No Hit
CAACTCTCTCCACCTAAATGTGGCACCTCCGGTTTGTTTAAAAGGCTCAC	8	0.2	No Hit
GGTAACTGTTGCTCACATAAACGCTACCGCAAAGAATGCTGCTGATGATA	8	0.2	No Hit
GGCAAATTCTGGATCTGCTACAAAAAACACAAACTTTAAAGGTTTACAGG	8	0.2	No Hit
GAGCTTTCCAGATTGCTATGCTGCGGAGTTTGGTGACCTAGAAATTACGC	8	0.2	No Hit
GGTTACCACTGAAGATTCCTTGCCACACTCTACTCTATGTTTATAACCTG	8	0.2	No Hit
AGAAAAAACAGAAATTCCAATACCAGTAAAGAAGGGGAACCTGTCCTGTA	8	0.2	No Hit
GTAACTATGTCTGTGGTTAACCAATGTGGCCTCATCCCATTTTTTAAAAA	8	0.2	No Hit
CTGAAGATTCCTTGCCACACTCTACTCTATGTTTATAACCTGCCGGCAAA	8	0.2	No Hit
GCTACTGAAGAGTCTGCCTTCTTTAGTAGAGGTTTTTATGAATGATAAGG	8	0.2	No Hit
GCAAGCAGGTCATTGTGAGCGATTTCTCACAGGCTTATCATTGGTGTTTC	8	0.2	No Hit
AACTTTTCTTTTGCACCTGTGACCAAGCTCTAACTTCCAGTTTGAACACA	8	0.2	No Hit
AGTGAACATTGCCACTGCTCAGAATGGCGTCAAAGTAGTGAAGTGGATTC	8	0.2	No Hit
GGAGGAGCTACTGGGAGCCATCCCACAGGTGGTCTGGATAAAAGGACATG	8	0.2	No Hit
CTATGATGTCAGTTCAGTCTCAGCTTTGACACAGAAGCTCTGCCATGTTG	8	0.2	No Hit
GCAAGAAAGGCCTGCTAGCCCGCGCCTCCCTGGGATCTTCGCCGCCCGAG	8	0.2	No Hit
GGGCACTGTGCCTTTGCTGAGCTTTCCAGATTGCTATGCTGCGGAGTTTG	8	0.2	No Hit
TGAACTTTTTCTTACTTTATTTCTTCCAGTTATTTGAAAGAACTCCATTA	8	0.2	No Hit
CGCTTCAGTGCCCACACTGAAAAACACAGGTGTGAGGGAGACACTTCACA	8	0.2	No Hit
CTGAAACTTCTCAAGTCCCAGGCCAGCAAGCAGGTCATTGTGAGCGATTT	8	0.2	No Hit
GGCTGGTCGGCAAGAAAGGCCTGCTAGCCCGCGCCTCCCTGGGATCTTCG	7	0.17500000000000002	No Hit
AGCTACCCATCTGCATTCAGCCAGCTGATCGCCTCAAGGCAAGTCAGTCC	7	0.17500000000000002	No Hit
TGTAATTTGAAGGCCAAGGTTGATCCTTGTTCTTGTGATTTAAAAATTGG	7	0.17500000000000002	No Hit
TTTAAACCAAGAAGAAAAAACAGAAATTCCAATACCAGTAAAGAAGGGGA	7	0.17500000000000002	No Hit
CATTTTGGAAAGCAGTGCCGGGTTTCAGACTATGGATATTCCAAGCTGAT	7	0.17500000000000002	No Hit
AGCTTACACCTCACTTGTGCACCAACTCTCTCCACCTAAATGTGGCACCT	7	0.17500000000000002	No Hit
CGAATCTGTGTGCAAGCAGCACTGCTCGAACAAGGACTTCAGCGAACTTG	7	0.17500000000000002	No Hit
CACGAGGGCACTGTGCCTTTGCTGAGCTTTCCAGATTGCTATGCTGCGGA	7	0.17500000000000002	No Hit
GTTATTTCATGCTTCCCTTCTCTGTCTGTCTCCGTTTGATAAGGGTAAGG	7	0.17500000000000002	No Hit
CCTCAAGGCAAGTCAGTCCTCTGTTCAGCTCTCAGTCTTGGTCTCCGAGG	7	0.17500000000000002	No Hit
CCGAGCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCCCATTTTACC	7	0.17500000000000002	No Hit
TCGCTGTGGAACAAAAGGGATGGAAAAGGAGACCTCGTTGTTGGCAACCA	7	0.17500000000000002	No Hit
GTGAACATTGCCACTGCTCAGAATGGCGTCAAAGTAGTGAAGTGGATTCA	7	0.17500000000000002	No Hit
TGAAAAACACAGGTGTGAGGGAGACACTTCACAGAAGCAGTCAAAAAAAA	7	0.17500000000000002	No Hit
ATTTAATTAAGAGCTTTCTAGAAAGTTATTTCATGCTTCCCTTCTCTGTC	7	0.17500000000000002	No Hit
CGCCGCCCGAGCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCCCAT	7	0.17500000000000002	No Hit
AGAAGAAAAAACAGAAATTCCAATACCAGTAAAGAAGGGGAACCTGTCCT	7	0.17500000000000002	No Hit
AGAAAGGCCTGCTAGCCCGCGCCTCCCTGGGATCTTCGCCGCCCGAGCTG	7	0.17500000000000002	No Hit
CTTCCAAATAAGCTACCCATCTGCATTCAGCCAGCTGATCGCCTCAAGGC	7	0.17500000000000002	No Hit
CTCCCAACACTGACCCTTGGCTTCTTCGTTCTAAAAGCCCTGTGAGAAAT	7	0.17500000000000002	No Hit
GCTAAAACTTTTGGTTATACATTTCGTCTCCAAGACTATGATGTCAGTTC	7	0.17500000000000002	No Hit
CTTCGCCGCCCGAGCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCC	7	0.17500000000000002	No Hit
TGAAGAGTCTGCCTTCTTTAGTAGAGGTTTTTATGAATGATAAGGCAGAA	7	0.17500000000000002	No Hit
CCAACACTGACCCTTGGCTTCTTCGTTCTAAAAGCCCTGTGAGAAATCCT	7	0.17500000000000002	No Hit
AGGCAAAGCTGCTTCTAAATTTGGGTCACCAGAAGTTGCAGTCACTGGAC	7	0.17500000000000002	No Hit
TTCCTTGCCACACTCTACTCTATGTTTATAACCTGCCGGCAAATAAAGAT	7	0.17500000000000002	No Hit
CTTTACTAAGTACAGAAACAATGTCTATTCTTCAGGATGCTCCTGCCTGT	7	0.17500000000000002	No Hit
CAGGTGAAGCGCTTTACTCAGGATTTACTGAAACTTCTCAAGTCCCAGGC	7	0.17500000000000002	No Hit
GATGTCAGTTCAGTCTCAGCTTTGACACAGAAGCTCTGCCATGTTGTAAA	7	0.17500000000000002	No Hit
GCGGAGAAGGAGCTGGTGGCTGAGGGTGGAAGAATGAAAGAAAAGAAAGA	7	0.17500000000000002	No Hit
CCAGTCTAGGCAAAGCTGCTTCTAAATTTGGGTCACCAGAAGTTGCAGTC	7	0.17500000000000002	No Hit
AAGGGCTGGTCGGCAAGAAAGGCCTGCTAGCCCGCGCCTCCCTGGGATCT	7	0.17500000000000002	No Hit
GGAATCTGTATTATATTTACCAAATTTTTCTAGACTATTGTGAATTGTGG	7	0.17500000000000002	No Hit
TTCCGATCTACCCCCCAGGTTACCACTGAAGATTCCTTGCCACACTCTAC	7	0.17500000000000002	No Hit
CCTCAAGTTCATAGTCTCCTGCAGACCCACGAGGGCACTGTGCCTTTGCT	7	0.17500000000000002	No Hit
TTCATCACCTGCATTCCAGGAGTGAACATTGCCACTGCTCAGAATGGCGT	6	0.15	No Hit
CTCTCAATGTGAAGATGTGTGTGTCCATGTTAGTTCATCTTGACGTCTTT	6	0.15	No Hit
GTGTGAGGGAGACACTTCACAGAAGCAGTCAAAAAAAAGAGAACCTCAGT	6	0.15	No Hit
CCAGAAGCCACTTTTATTAATGGTCCATAGGGAAATGTTTGTTTCCAACT	6	0.15	No Hit
AGGAGAAACACTGCAGCCCAAAACTTATGGCTACAGTAGTGTGGAGGAGC	6	0.15	No Hit
GCTGCTTCTAAATTTGGGTCACCAGAAGTTGCAGTCACTGGACAGGTGCT	6	0.15	No Hit
TGGAAATCCTTCCCAGTGGGGACCTCACAGAGATTGGTGAGAAGGGTGTG	6	0.15	No Hit
CGCTACCGCAAAGAATGCTGCTGATGATAAACTCCGTCAGAGCCTGCGAA	6	0.15	No Hit
GATAAACTCCGTCAGAGCCTGCGAAGGTTTGCAAATACGCACACTGCTCC	6	0.15	No Hit
GGGTAAGGTTTAAGGAAAAGAGATGGTCTCGATGTCATCCATTTAAGTCA	6	0.15	No Hit
GGTCATTTGTATCCGAAAAAGAGAACGAACTCAGGATGAAATAGAAAGGA	6	0.15	No Hit
TGTTACTGTTCACTGTTGGAAGCATGTTTGGGTTTTTATTATTATGGTAG	6	0.15	No Hit
ATTTGAAAGAACTCCATTAAGGAATCTGTATTATATTTACCAAATTTTTC	6	0.15	No Hit
CTCAAGGCAAGTCAGTCCTCTGTTCAGCTCTCAGTCTTGGTCTCCGAGGA	6	0.15	No Hit
GGTTTAAGGAAAAGAGATGGTCTCGATGTCATCCATTTAAGTCAGCATTT	6	0.15	No Hit
TGTCTCCGTTTGATAAGGGTAAGGTTTAAGGAAAAGAGATGGTCTCGATG	6	0.15	No Hit
CTGACCCTTGGCTTCTTCGTTCTAAAAGCCCTGTGAGAAATCCTCAGCTG	6	0.15	No Hit
CTGGGATCTTCGCCGCCCGAGCTGGACATCATGCCACAGGAAGAGTGAGC	6	0.15	No Hit
GTAGCCCAGCATCTGCTTCTGCTCCTCCCAAACCCTCCTCAGAAGCCCCC	6	0.15	No Hit
CACTGAAGATTCCTTGCCACACTCTACTCTATGTTTATAACCTGCCGGCA	6	0.15	No Hit
AGCCAAACATGTCCTTAAACCTGTGGGTCACACACTGGGGCATGATTGAG	6	0.15	No Hit
ACTTGATTGATATAATATCAGAGATCCCAGACACAACTATTTGCTTGTCC	6	0.15	No Hit
CAAAAATAGAGTCAAATTGGCAGCCAACTTTTCTTTTGCACCTGTGACCA	6	0.15	No Hit
GCTACTGGGAGCCATCCCACAGGTGGTCTGGATAAAAGGACATGGTCATA	6	0.15	No Hit
GTCTGTGGTTAACCAATGTGGCCTCATCCCATTTTTTAAAAAAGTTTCCA	6	0.15	No Hit
CAAGTTCATAGTCTCCTGCAGACCCACGAGGGCACTGTGCCTTTGCTGAG	6	0.15	No Hit
GCCAACTTTTCTTTTGCACCTGTGACCAAGCTCTAACTTCCAGTTTGAAC	6	0.15	No Hit
GGAACACCCTCAGGAGCCTTTGGGAGTTTTATTTTTAAACCAAGAAGAAA	6	0.15	No Hit
GCAAATGTATTTGTGGTTGTTCAAGAAGCCAAACATGTCCTTAAACCTGT	6	0.15	No Hit
CTCCGTCAGAGCCTGCGAAGGTTTGCAAATACGCACACTGCTCCAGCTAC	6	0.15	No Hit
GTCTGTCTCCGTTTGATAAGGGTAAGGTTTAAGGAAAAGAGATGGTCTCG	6	0.15	No Hit
CTGTTGCTCACATAAACGCTACCGCAAAGAATGCTGCTGATGATAAACTC	6	0.15	No Hit
GGTTAACCAATGTGGCCTCATCCCATTTTTTAAAAAAGTTTCCATTTGGG	6	0.15	No Hit
CTACTGGGAGCCATCCCACAGGTGGTCTGGATAAAAGGACATGGTCATAA	6	0.15	No Hit
CTCTCTTTGAAAGTGTTCGCACCTCAAGTTCATAGTCTCCTGCAGACCCA	6	0.15	No Hit
CAATGTGAAGATGTGTGTGTCCATGTTAGTTCATCTTGACGTCTTTAATA	6	0.15	No Hit
AGCTGATCAGATTTGAAGAGTTCATTTCCGATCTACCCCCCAGGTTACCA	6	0.15	No Hit
CAAGAAGATACTGGTGTCACTCTCCACAGGAGCTGCCAACAAGTCACTCT	6	0.15	No Hit
GGCCAGGTGAAGAGTGTTGAGCTCAGTCCCCATACTGATTACCAGCTCAA	6	0.15	No Hit
ATTGTAAATCAGCTGAGTGGTGAAGTGCTTCAAATGGTGTGGGTTCAATA	6	0.15	No Hit
TGGATATTCCAAGCTGATTGAGCTACTAGAAGCCGTGCCTCATGTGTTGC	6	0.15	No Hit
TCGGAATGTAAGCGGAGAAGGAGCTGGTGGCTGAGGGTGGAAGAATGAAA	6	0.15	No Hit
CTGAGGTATCCACTTATGAGTGGCCTCTCACCGCTGTTGGAGAGACCTGA	6	0.15	No Hit
TCTGAAGCCTTGTTACATCATGCTAACCAGCTGATCAGATTTGAAGAGTT	6	0.15	No Hit
GCAAGAAGATACTGGTGTCACTCTCCACAGGAGCTGCCAACAAGTCACTC	6	0.15	No Hit
ATGACTTTGACTGAGCTACTGAAGAGTCTGCCTTCTTTAGTAGAGGTTTT	6	0.15	No Hit
CAGACACAACTATTTGCTTGTCCCAACAAGATGATGACATGGTCATTTGT	6	0.15	No Hit
ACCTCAAGTTCATAGTCTCCTGCAGACCCACGAGGGCACTGTGCCTTTGC	6	0.15	No Hit
TTGGAAATTCTCTGGTTGCTTTTCTCTTCCTGAGCAGACATTGCCTCTTA	6	0.15	No Hit
TGAACATTGCCACTGCTCAGAATGGCGTCAAAGTAGTGAAGTGGATTCAC	5	0.125	No Hit
CTCCATTAAGGAATCTGTATTATATTTACCAAATTTTTCTAGACTATTGT	5	0.125	No Hit
CAAACATGTCCTTAAACCTGTGGGTCACACACTGGGGCATGATTGAGTGG	5	0.125	No Hit
GTTTCCAACTTTCAGATGAACTTTTTCTTACTTTATTTCTTCCAGTTATT	5	0.125	No Hit
ACTTCAGCGAACTTGTATTTGATCCAGACTCTTACAAGGTTCCTTTTGTG	5	0.125	No Hit
CCCTGGGATCTTCGCCGCCCGAGCTGGACATCATGCCACAGGAAGAGTGA	5	0.125	No Hit
CTGAAAAACACAGGTGTGAGGGAGACACTTCACAGAAGCAGTCAAAAAAA	5	0.125	No Hit
CCAAGCTCTAACTTCCAGTTTGAACACAGAATTAAATTAGGATGGGGGGA	5	0.125	No Hit
GGTCTCGATGTCATCCATTTAAGTCAGCATTTGAATGACATATTGTGTAT	5	0.125	No Hit
CTTTGAAGAGTTGGAGTATCACGAATCTGTGTGCAAGCAGCACTGCTCGA	5	0.125	No Hit
AAATAAGCAGAGATCGTTCAGCAGGAAGTGGATTGAGTCCCATTGAGCAG	5	0.125	No Hit
ACTCCATTAAGGAATCTGTATTATATTTACCAAATTTTTCTAGACTATTG	5	0.125	No Hit
CTCATGTGTTGCAGATTCTTGGAATGGGCTCCAAGCGTCTACTGACCCTC	5	0.125	No Hit
GAAACACTGCAGCCCAAAACTTATGGCTACAGTAGTGTGGAGGAGCTACT	5	0.125	No Hit
TTCCTGAGTAAAGCGCTTCACCTGTGCCCTATGAGTGAGGGTCAGTAGAC	5	0.125	No Hit
AGACTATTGTGAATTGTGGTGGTTGGGAAGAAATAAGCAGAGATCGTTCA	5	0.125	No Hit
GCATGTTTGGGTTTTTATTATTATGGTAGCTGATGTTGGCATTTGGGAAA	5	0.125	No Hit
GTCAGTTCAGTCTCAGCTTTGACACAGAAGCTCTGCCATGTTGTAAAGGT	5	0.125	No Hit
GGCCAGCAAGCAGGTCATTGTGAGCGATTTCTCACAGGCTTATCATTGGT	5	0.125	No Hit
AGACACTTCACAGAAGCAGTCAAAAAAAAGAGAACCTCAGTTCCCAGAGC	5	0.125	No Hit
GCGATTTCTCACAGGCTTATCATTGGTGTTTCTCAAAGGACTGGGATGTC	5	0.125	No Hit
CACCAACTCTCTCCACCTAAATGTGGCACCTCCGGTTTGTTTAAAAGGCT	5	0.125	No Hit
GGGAAAACTGTCTGATTCTACACACAAAAATAGGCTTAAAAACCTTATTT	5	0.125	No Hit
ACCATCACCATTTTGGAAAGCAGTGCCGGGTTTCAGACTATGGATATTCC	5	0.125	No Hit
GTTCAATATTATTTCCACTAAGAAGAAACCAGAGTTTTATCTGTAATTTG	5	0.125	No Hit
GTTTACAGGAGCTGTGCCGAATGGAACCAAAGTCTGGCAATAGAATCCGT	5	0.125	No Hit
TTCTTCATGAATTTGCCATGGCCTATACTAAATATGTAGGAGAAACACTG	5	0.125	No Hit
CACGAATCTGTGTGCAAGCAGCACTGCTCGAACAAGGACTTCAGCGAACT	5	0.125	No Hit
ACCAGTCTAGGCAAAGCTGCTTCTAAATTTGGGTCACCAGAAGTTGCAGT	5	0.125	No Hit
GCAGCCCAAAAATAGAGTCAAATTGGCAGCCAACTTTTCTTTTGCACCTG	5	0.125	No Hit
GACATTATGAAACCACTCACAGTACTCCTCTCAACCCCTGTGAATATGGT	5	0.125	No Hit
ACCAGTAAAGAAGGGGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAG	5	0.125	No Hit
AGAATATGCTAAAACTTTTGGTTATACATTTCGTCTCCAAGACTATGATG	5	0.125	No Hit
AACCGCTTCAGTGCCCACACTGAAAAACACAGGTGTGAGGGAGACACTTC	5	0.125	No Hit
GCTACTAGAAGCCGTGCCTCATGTGTTGCAGATTCTTGGAATGGGCTCCA	5	0.125	No Hit
GGGGACTGTCTTCCAAATAAGCTACCCATCTGCATTCAGCCAGCTGATCG	5	0.125	No Hit
TTATTTTTAAACCAAGAAGAAAAAACAGAAATTCCAATACCAGTAAAGAA	5	0.125	No Hit
GCCTTCTCTTCTGGGTGCCTGAGGTATCCACTTATGAGTGGCCTCTCACC	5	0.125	No Hit
GGCCTATACTAAATATGTAGGAGAAACACTGCAGCCCAAAACTTATGGCT	5	0.125	No Hit
GTCATTTGTATCCGAAAAAGAGAACGAACTCAGGATGAAATAGAAAGGAC	5	0.125	No Hit
GGTCATAAGAGAATTGTAGTGTTAAAAAATGACATGAAAAACCGGCTAAG	5	0.125	No Hit
GTTAGAGTCTGATTTTTTAAACTATGCATTAAATGCATAAATTGTAAATC	5	0.125	No Hit
GAAGCAGTCAAAAAAAAGAGAACCTCAGTTCCCAGAGCATTGCCAGCTCT	5	0.125	No Hit
GGTTTACAGGAGCTGTGCCGAATGGAACCAAAGTCTGGCAATAGAATCCG	5	0.125	No Hit
AAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGCCAACTTTTCTTTTG	5	0.125	No Hit
AGACTATGGATATTCCAAGCTGATTGAGCTACTAGAAGCCGTGCCTCATG	5	0.125	No Hit
GAAATTCCAATACCAGTAAAGAAGGGGAACCTGTCCTGTAACTCCTCACC	5	0.125	No Hit
CTGCTGATGATAAACTCCGTCAGAGCCTGCGAAGGTTTGCAAATACGCAC	5	0.125	No Hit
GGGAAAACTGTCTGATTCTACACACAAAAATAGGCTTAAAAACCTTATTTCATGTCTTGACCCCAGA	5	0.125	No Hit
GGGAAATGTTTGTTTCCAACTTTCAGATGAACTTTTTCTTACTTTATTTC	5	0.125	No Hit
GTGGCAAGAGAGCCATTTTCCGGTTGACAGGAAAGTTCCTGGCTTCTAGG	5	0.125	No Hit
AGACCAGCCTGCAGTCAGCAGGCAGATCCTGGAGGTGCCAGAACCCCATC	5	0.125	No Hit
GATTCCATTTAATTAAGAGCTTTCTAGAAAGTTATTTCATGCTTCCCTTC	5	0.125	No Hit
GCCCAGCAAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGCCAACTTT	5	0.125	No Hit
AGCAAATGTATTTGTGGTTGTTCAAGAAGCCAAACATGTCCTTAAACCTG	5	0.125	No Hit
GCTAACCAGCTGATCAGATTTGAAGAGTTCATTTCCGATCTACCCCCCAG	5	0.125	No Hit
AAAAAACAGAAATTCCAATACCAGTAAAGAAGGGGAACCTGTCCTGTAAC	5	0.125	No Hit
ATTAAATGCATAAATTGTAAATCAGCTGAGTGGTGAAGTGCTTCAAATGG	5	0.125	No Hit
CTGCAGCCCAAAACTTATGGCTACAGTAGTGTGGAGGAGCTACTGGGAGC	5	0.125	No Hit
CTCTTAGTTCCCAAACGAAAGAGTACATGGAGAACAAGAAAGCTGCTGTG	5	0.125	No Hit
CAGAGAAGCAGAGTTTATCTGTGTGTGTGACATCAGTAAAGAGAACAAGG	5	0.125	No Hit
GGTAAGGTTTAAGGAAAAGAGATGGTCTCGATGTCATCCATTTAAGTCAG	5	0.125	No Hit
AGCATGGCCAGGTGAAGAGTGTTGAGCTCAGTCCCCATACTGATTACCAG	5	0.125	No Hit
GGATTGAGTCCCATTGAGCAGCCTCCATCATTTGTTTAAGCAAATGTATT	5	0.125	No Hit
CAAAGCTGCTTCTAAATTTGGGTCACCAGAAGTTGCAGTCACTGGACAGG	5	0.125	No Hit
ACTGAAGATTCCTTGCCACACTCTACTCTATGTTTATAACCTGCCGGCAA	5	0.125	No Hit
GAATGGCGTCAAAGTAGTGAAGTGGATTCACAACAAGCCCCCACCTCCCA	5	0.125	No Hit
CACCCTCAGGAGCCTTTGGGAGTTTTATTTTTAAACCAAGAAGAAAAAAC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138-139	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCCAG	45	0.007030047	51.231884	110-114
>>END_MODULE
SRR26281818 read2 length is 39-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR26281818_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	39-151
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.077	37.0	37.0	37.0	37.0	37.0
2	36.2485	37.0	37.0	37.0	37.0	37.0
3	36.2325	37.0	37.0	37.0	37.0	37.0
4	36.332	37.0	37.0	37.0	37.0	37.0
5	36.193	37.0	37.0	37.0	37.0	37.0
6	36.3035	37.0	37.0	37.0	37.0	37.0
7	36.2365	37.0	37.0	37.0	37.0	37.0
8	36.2665	37.0	37.0	37.0	37.0	37.0
9	36.1965	37.0	37.0	37.0	37.0	37.0
10-14	36.2561	37.0	37.0	37.0	37.0	37.0
15-19	36.2169	37.0	37.0	37.0	37.0	37.0
20-24	36.2478	37.0	37.0	37.0	37.0	37.0
25-29	36.2179	37.0	37.0	37.0	37.0	37.0
30-34	36.1688	37.0	37.0	37.0	37.0	37.0
35-39	36.0951	37.0	37.0	37.0	37.0	37.0
40-44	36.11415707853927	37.0	37.0	37.0	37.0	37.0
45-49	36.09054527263631	37.0	37.0	37.0	37.0	37.0
50-54	36.11085542771386	37.0	37.0	37.0	37.0	37.0
55-59	36.03386289091505	37.0	37.0	37.0	37.0	37.0
60-64	35.977875787999075	37.0	37.0	37.0	37.0	37.0
65-69	36.01349140793617	37.0	37.0	37.0	37.0	37.0
70-74	35.9817276843625	37.0	37.0	37.0	37.0	37.0
75-79	35.95242743323955	37.0	37.0	37.0	37.0	37.0
80-84	35.96851223545094	37.0	37.0	37.0	37.0	37.0
85-89	35.94681382331474	37.0	37.0	37.0	37.0	37.0
90-94	35.926406790890546	37.0	37.0	37.0	37.0	37.0
95-99	35.907234590021055	37.0	37.0	37.0	37.0	37.0
100-104	35.841695021509565	37.0	37.0	37.0	37.0	37.0
105-109	35.85082926429634	37.0	37.0	37.0	37.0	37.0
110-114	35.75112450168423	37.0	37.0	37.0	37.0	37.0
115-119	35.79379857526452	37.0	37.0	37.0	37.0	37.0
120-124	35.76494234670126	37.0	37.0	37.0	37.0	37.0
125-129	35.6319884969836	37.0	37.0	37.0	37.0	37.0
130-134	35.62152106753052	37.0	37.0	37.0	37.0	37.0
135-139	35.39989067068531	37.0	37.0	37.0	34.6	37.0
140-144	35.4095333689016	37.0	37.0	37.0	37.0	37.0
145-149	35.22432233369919	37.0	37.0	37.0	27.4	37.0
150-151	35.647527187162154	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	2.0
25	5.0
26	10.0
27	18.0
28	23.0
29	25.0
30	40.0
31	50.0
32	77.0
33	117.0
34	171.0
35	441.0
36	2592.0
37	429.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.125	10.25	16.25	39.375
2	25.4	7.000000000000001	33.050000000000004	34.55
3	25.1	10.25	25.900000000000002	38.75
4	24.85	15.575	20.25	39.324999999999996
5	26.275	22.775000000000002	28.275	22.675
6	30.575000000000003	21.425	23.95	24.05
7	18.8	27.375	38.475	15.35
8	21.65	24.15	33.6	20.599999999999998
9	20.95	23.849999999999998	34.375	20.825
10-14	23.91	27.67	28.405	20.015
15-19	22.71	29.080000000000002	28.46	19.75
20-24	22.2	25.05	31.245	21.505
25-29	22.685	27.700000000000003	27.075	22.54
30-34	22.52	26.76	28.660000000000004	22.06
35-39	23.425	26.669999999999998	28.825	21.08
40-44	23.5767883941971	28.3591795897949	27.78389194597299	20.280140070035017
45-49	22.746373186593296	26.24312156078039	27.978989494747374	23.03151575787894
50-54	23.901950975487743	26.91345672836418	25.527763881940967	23.656828414207105
55-59	21.47180949522237	26.884786632647955	28.21051578368102	23.432888088448646
60-64	21.988585160708922	28.036447381596076	26.63462501251627	23.340342445178734
65-69	21.90887193898033	27.308309915696505	27.629466077880373	23.153352067442796
70-74	24.019731212563546	26.55156792671264	26.244526098555394	23.184174762168418
75-79	23.28601020562825	24.75117465770727	28.722275551962817	23.24053958470166
80-84	21.48882113821138	26.438008130081304	29.6900406504065	22.383130081300813
85-89	21.573160706946158	28.05692560624743	28.606658446362516	21.763255240443897
90-94	23.29009741105381	28.97848622180549	26.920873053081213	20.810543314059487
95-99	23.789529630613874	28.312516609088494	25.585968642040925	22.31198511825671
100-104	24.294465106103697	26.115729599649967	28.204987967621964	21.384817326624372
105-109	23.451427281958708	27.518898792733836	27.693783143405167	21.33589078190229
110-114	23.061653432975245	26.856609061186358	28.041802895843066	22.039934609995328
115-119	25.098682212910674	24.795044634723993	28.080403230703833	22.025869921661506
120-124	21.77814548463056	26.608540698784445	29.05237701266467	22.56093680392032
125-129	22.163588390501317	25.22833367160544	28.137473783911776	24.470604153981462
130-134	21.291473421334285	26.350338922582946	30.788440956118446	21.569746699964323
135-139	18.98449786564817	26.510896427769044	31.56594023814873	22.93866546843406
140-144	19.834254143646408	29.021310181531174	28.295185477505918	22.849250197316493
145-149	20.310532941670164	29.609735627360468	26.39530004196391	23.684431389005457
150-151	21.684640046633636	12.56193529583212	39.14310696589916	26.610317691635093
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.5
27	0.5
28	4.5
29	6.5
30	12.5
31	24.0
32	43.5
33	51.5
34	59.0
35	77.0
36	81.0
37	114.5
38	140.0
39	128.0
40	149.0
41	175.5
42	188.0
43	203.5
44	215.5
45	224.5
46	209.0
47	169.5
48	161.5
49	193.0
50	198.5
51	189.0
52	189.5
53	174.5
54	135.0
55	87.0
56	107.0
57	129.5
58	94.0
59	62.0
60	26.5
61	6.0
62	1.5
63	0.0
64	0.0
65	2.5
66	2.5
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0050062578222778474
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.005468963631391851
105-109	0.0
110-114	0.005838052425710783
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.007488392990864161
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35-39	2.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	1.0
60-64	3.0
65-69	16.0
70-74	11.0
75-79	22.0
80-84	30.0
85-89	56.0
90-94	55.0
95-99	109.0
100-104	93.0
105-109	137.0
110-114	116.0
115-119	145.0
120-124	163.0
125-129	187.0
130-134	129.0
135-139	145.0
140-144	130.0
145-149	399.0
150-152	2051.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.574999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	45.69021386908619	17.625
2	19.572261827608557	15.1
3	13.933895009721322	16.125
4	8.230719377835385	12.7
5	4.795852235904083	9.25
6	2.268308489954634	5.25
7	1.6202203499675956	4.375
8	0.7777057679844459	2.4
9	0.9073233959818535	3.15
>10	2.138690861957226	12.125
>50	0.06480881399870382	1.9
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACCTCC	76	1.9	No Hit
CCCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGTC	28	0.7000000000000001	No Hit
CCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGTCA	28	0.7000000000000001	No Hit
CCTCGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTTCT	22	0.5499999999999999	No Hit
CCTCTCAATACACAATATGTCATTCAAATGCTGACTTAAATGGATGACAT	22	0.5499999999999999	No Hit
CACCACTCAGCTGATTTACAATTTATGCATTTAATGCATAGTTTAAAAAA	22	0.5499999999999999	No Hit
GCTGACTTAAATGGATGACATCGAGACCATCTCTTTTCCTTAAACCTTAC	21	0.525	No Hit
CCCCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGT	21	0.525	No Hit
CGGAAATGAACTCTTCAAATCTGATCAGCTGGTTAGCATGATGTAACAAG	19	0.475	No Hit
GTCCGCAAAGGCTAGCTTTTCCCAAATGCCAACATCAGCTACCATAATAA	17	0.42500000000000004	No Hit
GCGGTGTTCAAAAGGTTTGGAGACATACTCCTCGGAGACCAAGACTGAGA	17	0.42500000000000004	No Hit
GCCGGTTTTTCATGTCATTTTTTAACACTACAATTCTCTTATGACCATGT	16	0.4	No Hit
CTCACGGATTCTCTGTACAACAGTTGTTGCTGACCGGCCAGAGGGGACTG	15	0.375	No Hit
GTACAAAGGGATGAAATTACTGATTGGTAGTATGCAAGATGGTTGGTTCT	15	0.375	No Hit
CCGGTTTTTCATGTCATTTTTTAACACTACAATTCTCTTATGACCATGTC	14	0.35000000000000003	No Hit
CCCTTATCAAACGGAGACAGACAGAGAAGGGAAGCATGAAATAACTTTCT	14	0.35000000000000003	No Hit
GGTCAGTAGACGCTTGGAGCCCATTCCAAGAATCTGCAACACATGAGGCA	13	0.325	No Hit
CCCGGCACTGCTTTCCAAAATGGTGATGGTACAAAGGGATGAAATTACTG	13	0.325	No Hit
GCCACATTGGTTAACCACAGACATAGTTACATAAAAAACAAGGCACACCA	12	0.3	No Hit
GGGGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAGAAT	12	0.3	No Hit
GTCTCCCTCACACCTGTGTTTTTCAGTGTGGGCACTGAAGCGGTTGGGCC	11	0.27499999999999997	No Hit
GGGCTGGTGGCGCAGCAAGTCCACAACATCTTTGGAGAACTGCTTTGTCC	11	0.27499999999999997	No Hit
GTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAGAATCAG	11	0.27499999999999997	No Hit
CCACTCAGCTGATTTACAATTTATGCATTTAATGCATAGTTTAAAAAATC	11	0.27499999999999997	No Hit
CCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACCTCCAG	10	0.25	No Hit
CTCCTCTGATCCAAAAGTCTTTCCTGAGTCCTCCATGGTAAAATGGGCAA	10	0.25	No Hit
CGGCAGGTTATAAACATAGAGTAGAGTGTGGCAAGGAATCTTCAGTGGTA	10	0.25	No Hit
CCCAAATGGAAACTTTTTTAAAAAATGGGATGAGGCCACATTGGTTAACC	10	0.25	No Hit
TCACCGTATTCCGTGACATCCCAGTCCTTTGAGAAACACCAATGATAAGC	10	0.25	No Hit
CTCCAGGATCTGCCTGCTGACTGCAGGCTGGTCTTCATGATTGACAGGGG	10	0.25	No Hit
CCACTGCATCCTTGCTAATCAGTAGCCCGGGGGCTTCTGAGGAGGGTTTG	10	0.25	No Hit
CTGGGGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAGA	10	0.25	No Hit
GCTCTTGCCATCTTTATTTGCCGGCAGGTTATAAACATAGAGTAGAGTGT	10	0.25	No Hit
GCCCTATGAGTGAGGGTCAGTAGACGCTTGGAGCCCATTCCAAGAATCTG	10	0.25	No Hit
AGGGCAATGAGCACAGCAGCTTACTTTGGGAATAGCTGAGAGCTGTACTT	9	0.22499999999999998	No Hit
GCCCCGGTGTGTGACCCACAGGTTTAAGGACATGTTTGGCTTCTTGAACA	9	0.22499999999999998	No Hit
GCCAGATTTTAGCAGCATCAATTATACTATTTCTTGCTGGCTTGTTTAGA	9	0.22499999999999998	No Hit
TCCCCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGG	9	0.22499999999999998	No Hit
CCTTCAAATTACAGATAAAACTCTGGTTTCTTCTTAGTGGAAATAATATT	9	0.22499999999999998	No Hit
CTGCTTTTTTGCTGGGCTTTCCACTGCATCCTTGCTAATCAGTAGCCCGG	9	0.22499999999999998	No Hit
GTTTGGGAGGAGCAGAAGCAGATGCTGGGCTACTGGGTGAGGAGTTACAG	9	0.22499999999999998	No Hit
CCCCAGTGTGTGACCCACAGGTTTAAGGACATGTTTGGCTTCTTGAACAA	9	0.22499999999999998	No Hit
GTGGCTTCTGGGGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGT	9	0.22499999999999998	No Hit
GGTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACCTC	9	0.22499999999999998	No Hit
GGCGAGGCGGTGTTCAAAAGGTTTGGAGACATACTCCTCGGAGACCAAGA	9	0.22499999999999998	No Hit
CCGGCAGGTTATAAACATAGAGTAGAGTGTGGCAAGGAATCTTCAGTGGT	9	0.22499999999999998	No Hit
TGGTAGTATGCAAGATGGTTGGTTCTTTAGTAAATCAATGACTTCTCTGC	9	0.22499999999999998	No Hit
CTTCAAATTACAGATAAAACTCTGGTTTCTTCTTAGTGGAAATAATATTG	9	0.22499999999999998	No Hit
CACTCAATCATGCCCCAGTGTGTGACCCACAGGTTTAAGGACATGTTTGG	8	0.2	No Hit
AACCCGGCACTGCTTTCCAAAATGGTGATGGTACAAAGGGATGAAATTAC	8	0.2	No Hit
TTGTTGTGAATCCACTTCACTACTTTGACGCCATTCTGAGCAGTGGCAAT	8	0.2	No Hit
CTCTAAGGGGACACCTTTGTCTCTGTCTTGCGTAATTTCTAGGTCACCAA	8	0.2	No Hit
CTGCTGCAAGGGTTCTCAGTTCCGTTCCCTTCCATCACATAGCCAGACAA	8	0.2	No Hit
CCTGGGACTTGAGAAGTTTCAGTAAATCCTGAGTAAAGCGCTTCACCTGT	8	0.2	No Hit
CCTCCTCTGATCCAAAAGTCTTTCCTGAGTCCTCCATGGTAAAATGGGCA	8	0.2	No Hit
CCGGGTTATCAGTTGGATTTGTTTGCCAGACTCCATATCAGCAACCTTTA	8	0.2	No Hit
ACCGGTTTTTCATGTCATTTTTTAACACTACAATTCTCTTATGACCATGT	8	0.2	No Hit
GGTTGGTTCTTTAGTAAATCAATGACTTCTCTGCTGAACTGAATCAGCTG	8	0.2	No Hit
CGCCTGTGATACTTAGCACCTTGCCACCACAGTTATCAGAGAGGCGCCTG	8	0.2	No Hit
GGTTGCTGAAGAGAATGAATATCAGGAAGGGGAACTGCTGGGAAAAATTT	8	0.2	No Hit
GGGGGTAGATCGGAAATGAACTCTTCAAATCTGATCAGCTGGTTAGCATG	7	0.17500000000000002	No Hit
CTGCTTGCTGGCCTGGGACTTGAGAAGTTTCAGTAAATCCTGAGTAAAGC	7	0.17500000000000002	No Hit
CGGCGGTGTTCAAAAGGTTTGGAGACATACTCCTCGGAGACCAAGACTGA	7	0.17500000000000002	No Hit
CCCGGTTTCTGGTTTCAGCTTGCTGCAGCTGAGTCTCCAATTCAGCTTCA	7	0.17500000000000002	No Hit
GTTCTGGCACCTCCAGGATCTGCCTGCTGACTGCAGGCTGGTCTTCATGA	7	0.17500000000000002	No Hit
CTCAATACACAATATGTCATTCAAATGCTGACTTAAATGGATGACATCGA	7	0.17500000000000002	No Hit
CTCCCGGCTTCCTTCCTGGAATTCTCGGAGTTCCTCCTGAGTATTCTCTG	7	0.17500000000000002	No Hit
CACACCATTTGAAGCACTTCACCACTCAGCTGATTTACAATTTATGCATT	7	0.17500000000000002	No Hit
GCCAATTTGACTCTATTTTTGGGCTGCTTTTTTGCTGGGCTTTCCACTGC	7	0.17500000000000002	No Hit
CCCAGATTTACAGCCAATTTTTAAATCACAAGAACAAGGATCAACCTTGG	7	0.17500000000000002	No Hit
CCTGGGGGGTAGATCGGAAATGAACTCTTCAAATCTGATCAGCTGGTTAG	7	0.17500000000000002	No Hit
CTGGCTGGGTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTG	7	0.17500000000000002	No Hit
CCTGAGATCACTGAGTTCTAATGCAAAATTAACATCAGTTGACACAAGAA	7	0.17500000000000002	No Hit
GTTGAGAGGAGTACTGTGAGTGGTTTCATAATGTCTCTTGAGCTCATCAA	7	0.17500000000000002	No Hit
CTTCTGGGGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGT	7	0.17500000000000002	No Hit
GCCGGCCAAAGTGGTGGTGGTAAGAGGGAATGAACTTGTTAAAGGGCATT	7	0.17500000000000002	No Hit
CCAGATTTACAGCCAATTTTTAAATCACAAGAACAAGGATCAACCTTGGC	7	0.17500000000000002	No Hit
CTGTGTGTAGAATCAGACAGTTTTCCCCCCATCCTAATTTAATTCTGTGT	7	0.17500000000000002	No Hit
TACCTCTCAATACACAATATGTCATTCAAATGCTGACTTAAATGGATGAC	7	0.17500000000000002	No Hit
GTTCTTTAGTAAATCAATGACTTCTCTGCTGAACTGAATCAGCTGAGGAT	7	0.17500000000000002	No Hit
CCTGCTTGCTGGCCTGGGACTTGAGAAGTTTCAGTAAATCCTGAGTAAAG	7	0.17500000000000002	No Hit
TGGGTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACC	7	0.17500000000000002	No Hit
GGCTGCTTTTTTGCTGGGCTTTCCACTGCATCCTTGCTAATCAGTAGCCC	7	0.17500000000000002	No Hit
GTGGTAAGAGGGAATGAACTTGTTAAAGGGCATTCGGAAATGGGGCTGGT	7	0.17500000000000002	No Hit
CCCCTCTCTCTTTTTCTTCTGTGCAGGAGAGCTGGCAATGCTCTGGGAAC	7	0.17500000000000002	No Hit
CTCGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTTCTC	6	0.15	No Hit
CCCAAACATGCTTCCAACAGTGAACAGTAACAGTAAAGCTAGACACTATT	6	0.15	No Hit
CCGGCCAGAGGGGACTGAGCAGTTTTCAATATCCCAGAAAACACCAATGG	6	0.15	No Hit
CGGGCCTGGTTACTGGGTAAGAGACACACTAGTCGCCCATTTCCCTGTTC	6	0.15	No Hit
CTTCACATTGAGAGCTTCCCAGATTTACAGCCAATTTTTAAATCACAAGA	6	0.15	No Hit
GCCGCAAAGACTTCCGGGTTATCAGTTGGATTTGTTTGCCAGACTCCATA	6	0.15	No Hit
ACCCGGCACTGCTTTCCAAAATGGTGATGGTACAAAGGGATGAAATTACT	6	0.15	No Hit
GCTGGGTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCA	6	0.15	No Hit
TCCAAGTGTTCTGCTGCAAGGGTTCTCAGTTCCGTTCCCTTCCATCACAT	6	0.15	No Hit
GGGAGGTGGGGGCTTGTTGTGAATCCACTTCACTACTTTGACGCCATTCT	6	0.15	No Hit
CTGGTTACTGGGTAAGAGACACACTAGTCGCCCATTTCCCTGTTCACGGA	6	0.15	No Hit
AGGCGCCTGAGCCTGTTGCTGATGCTCTTGCCATCTTTATTTGCCGGCAG	6	0.15	No Hit
CCTTAATGGAGTTCTTTCAAATAACTGGAAGAAATAAAGTAAGAAAAAGT	6	0.15	No Hit
AGCTTATTTGGAAGACAGTCCCCTCTCTCTTTTTCTTCTGTGCAGGAGAG	6	0.15	No Hit
GCGCTACTGGGATTGGCAATGTTGAAAGCAAGTGGCGAGGCGGTGTTCAA	6	0.15	No Hit
CCACAAGGCAGTGTCCGCAAAGGCTAGCTTTTCCCAAATGCCAACATCAG	6	0.15	No Hit
GTCCTGTTCTGAGATGGAGCTCTGAGGCTGGATGGGGTTCTGGCACCTCC	6	0.15	No Hit
GGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAGAATCA	6	0.15	No Hit
ATAGCCAGACAATATGGTTCAACATTCTTTCATCTTTCTTTTCTTTCATT	6	0.15	No Hit
GCGGGCCTGGTTACTGGGTAAGAGACACACTAGTCGCCCATTTCCCTGTT	6	0.15	No Hit
GTGGGTCTGCAGGAGACTATGAACTTGAGGTGCGAACACTTTCAAAGAGA	6	0.15	No Hit
GCCATCTTTATTTGCCGGCAGGTTATAAACATAGAGTAGAGTGTGGCAAG	6	0.15	No Hit
CTGTGTTCAAACTGGAAGTTAGAGCTTGGTCACAGGTGCAAAAGAAAAGT	6	0.15	No Hit
TTCCCAAATGCCAACATCAGCTACCATAATAATAAAAACCCAAACATGCT	6	0.15	No Hit
GTTTTTATGTACCAAAATGATGTGGAAACCATGCCGGTGCCTGAGATCAC	6	0.15	No Hit
GGCGGTGTTCAAAAGGTTTGGAGACATACTCCTCGGAGACCAAGACTGAG	6	0.15	No Hit
TGGTTGGTTCTTTAGTAAATCAATGACTTCTCTGCTGAACTGAATCAGCT	6	0.15	No Hit
CCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACCTCCA	6	0.15	No Hit
CGGCACTGCTTTCCAAAATGGTGATGGTACAAAGGGATGAAATTACTGAT	6	0.15	No Hit
GATGGCTTTGAGCTGGTAATCAGTATGGGGACTGAGCTCAACACTCTTCA	6	0.15	No Hit
GGGTAGTATGCAAGATGGTTGGTTCTTTAGTAAATCAATGACTTCTCTGC	6	0.15	No Hit
GCACACCAATAAGTCACCTAGAAGCCAGGAACTTTCCTGTCAACCGGAAA	6	0.15	No Hit
CGCCGGCAGGTTATAAACATAGAGTAGAGTGTGGCAAGGAATCTTCAGTG	6	0.15	No Hit
GGCTTCTGGGGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGT	6	0.15	No Hit
CGGCCAAAGTGGTGGTGGTAAGAGGGAATGAACTTGTTAAAGGGCATTCG	6	0.15	No Hit
GTCACCTAGAAGCCAGGAACTTTCCTGTCAACCGGAAAATGGCTCTCTTG	5	0.125	No Hit
CTCTGATCCAAAAGTCTTTCCTGAGTCCTCCATGGTAAAATGGGCAAGAG	5	0.125	No Hit
GGGGTCGTCCAAGAGGTAGATGTCAGAGTTACAATACACAGCCCGAGCCA	5	0.125	No Hit
GCCAGACTTTGGTTCCATTCGGCACAGCTCCTGTAAACCTTTAAAGTTTG	5	0.125	No Hit
CTTCCCAAGACATCAACACCACCAGCAACTGAACAGTGAGAGGCCGCAAA	5	0.125	No Hit
GGCAAGGAATCTTCAGTGGTAACCTGGGGGGTAGATCGGAAATGAACTCT	5	0.125	No Hit
GGTCTGTGGGATGGCTCCCAGTAGCTCCTCCACACTACTGTAGCCATAAG	5	0.125	No Hit
GAAGCACTTCACCACTCAGCTGATTTACAATTTATGCATTTAATGCATAG	5	0.125	No Hit
AGCGGTGTTCAAAAGGTTTGGAGACATACTCCTCGGAGACCAAGACTGAG	5	0.125	No Hit
GCACCGTATTCCGTGACATCCCAGTCCTTTGAGAAACACCAATGATAAGC	5	0.125	No Hit
GCAGGCTGAGTACTTGGAGGTGGTATATTTGGCCAGATTTTAGCAGCATC	5	0.125	No Hit
GTTATTTTGGTGTAACCATCCAAGTGTTCTGCTGCAAGGGTTCTCAGTTC	5	0.125	No Hit
TGCCGGCAGGTTATAAACATAGAGTAGAGTGTGGCAAGGAATCTTCAGTG	5	0.125	No Hit
ATCCAGACCACCTGTGGGATGGCTCCCAGTAGCTCCTCCACACTACTGTA	5	0.125	No Hit
GTGCGAACACTTTCAAAGAGAGAACCACAAAAGGAACCTTGTAAGAGTCT	5	0.125	No Hit
CGGCTGCCAATTTGACTCTATTTTTGGGCTGCTTTTTTGCTGGGCTTTCC	5	0.125	No Hit
GTAATTTCTAGGTCACCAAACTCCGCAGCATAGCAATCTGGAAAGCTCAG	5	0.125	No Hit
GCCAACAGGTTCTCTCTGCTCTATGAATGGATTTGCCCTTTTTGCCCAAC	5	0.125	No Hit
CTCTCCAATAGTTTGTTTCTTCCTCCTCTGATCCAAAAGTCTTTCCTGAG	5	0.125	No Hit
CGGGTCTGAGCAGTCAGCAGCGCTACTGGGATTGGCAATGTTGAAAGCAA	5	0.125	No Hit
GTGCCAACAGGTTCTCTCTGCTCTATGAATGGATTTGCCCTTTTTGCCCA	5	0.125	No Hit
TCCTGAGTTCGTTCTCTTTTTCGGATACAAATGACCATGTCATCATCTTG	5	0.125	No Hit
GTTATTCTCTGATAATAGGTCCCGGTTTCTGGTTTCAGCTTGCTGCAGCT	5	0.125	No Hit
GTTGGGAGGTGGGGGCTTGTTGTGAATCCACTTCACTACTTTGACGCCAT	5	0.125	No Hit
GTTAATTTATATAAATCAGACACATTTAACTTGTGTCCATACTTCTTTTC	5	0.125	No Hit
CTGCTGGTAGTGATAGGTATGAAGCAAAGACCTCACATTCTTTGCAAACA	5	0.125	No Hit
GCTGGGCTTTCCACTGCATCCTTGCTAATCAGTAGCCCGGGGGCTTCTGA	5	0.125	No Hit
GGGGCTGGTGGCGCAGCAAGTCCACAACATCTTTGGAGAACTGCTTTGTC	5	0.125	No Hit
ATAGTTACATAAAAAACAAGGCACACCAATAAGTCACCTAGAAGCCAGGA	5	0.125	No Hit
TGGGGGGTAGATCGGAAATGAACTCTTCAAATCTGATCAGCTGGTTAGCA	5	0.125	No Hit
GTTTCTTCTTAGTGGAAATAATATTGAACCCACACCATTTGAAGCACTTC	5	0.125	No Hit
ATCTGAAAGTTGGAAACAAACATTTCCCTATGGACCATTAATAAAAGTGG	5	0.125	No Hit
TGCCGGTTTTTCATGTCATTTTTTAACACTACAATTCTCTTATGACCATG	5	0.125	No Hit
GCTCAGCAAAGGCACAGTGCCCTCGTGGGTCTGCAGGAGACTATGAACTT	5	0.125	No Hit
GGCCACCACAGTTATCAGAGAGGCGCCTGAGCCTGTTGCTGATGCTCTTG	5	0.125	No Hit
CTGCCGGCCAAAGTGGTGGTGGTAAGAGGGAATGAACTTGTTAAAGGGCA	5	0.125	No Hit
CTCTCTTGCCACTCAATCATGCCCCAGTGTGTGACCCACAGGTTTAAGGA	5	0.125	No Hit
ATTACCTCTCAATACACAATATGTCATTCAAATGCTGACTTAAATGGATG	5	0.125	No Hit
TTTCCGTGACATCCCAGTCCTTTGAGAAACACCAATGATAAGCCTGTGAG	5	0.125	No Hit
CTGCATTCCAGGAGTGAACATTGCCACTGCTCAGAATGGCGTCAAAGTAG	5	0.125	No Hit
CTTGTTGTGAATCCACTTCACTACTTTGACGCCATTCTGAGCAGTGGCAA	5	0.125	No Hit
CCAGTGTGTGACCCACAGGTTTAAGGACATGTTTGGCTTCTTGAACAACC	5	0.125	No Hit
ACGGATTCTCTGTACAACAGTTGTTGCTGACCGGCCAGAGGGGACTGAGC	5	0.125	No Hit
CGGCCAGAGGGGACTGAGCAGTTTTCAATATCCCAGAAAACACCAATGGG	5	0.125	No Hit
AGCGCTACTGGGATTGGCAATGTTGAAAGCAAGTGGCGAGGCGGTGTTCA	5	0.125	No Hit
TCTGGGATTGGCAATGTTGAAAGCAAGTGGCGAGGCGGTGTTCAAAAGGT	5	0.125	No Hit
GCCAGGTCTCTCCAATAGTTTGTTTCTTCCTCCTCTGATCCAAAAGTCTT	5	0.125	No Hit
GGGTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACCT	5	0.125	No Hit
TTACCCTTATCAAACGGAGACAGACAGAGAAGGGAAGCATGAAATAACTT	5	0.125	No Hit
GTGCAGGAGAGCTGGCAATGCTCTGGGAACTGAGGTTCTCTTTTTTTTGA	5	0.125	No Hit
TCCTGCTCCATCTGGCTGGGTCCTGTTCTGAGATGGAGCTCTGAGGCTAG	5	0.125	No Hit
TCGCTGACTTAAATGGATGACATCGAGACCATCTCTTTTCCTTAAACCTT	5	0.125	No Hit
CTCTTGAATGACTTCCTTGTTCTCTTTACTGATGTCACACACACAGATAA	5	0.125	No Hit
TTGCCAATTTGACTCTATTTTTGGGCTGCTTTTTTGCTGGGCTTTCCACT	5	0.125	No Hit
CGGAAAATGGCTCTCTTGCCACTCAATCATGCCCCAGTGTGTGACCCACA	5	0.125	No Hit
CTTCCTCCTCTGATCCAAAAGTCTTTCCTGAGTCCTCCATGGTAAAATGG	5	0.125	No Hit
GGACTGAGCTCAACACTCTTCACCTGGCCATGCTTGGAGAAGGCTTCTTG	5	0.125	No Hit
GCCACCAGCAACTGAACAGTGAGAGGCCGCAAAGACTTCCGGGTTATCAG	5	0.125	No Hit
GTGGCAATGTTCACTCCTGGAATGCAGGTGATGAAGTGCTCTAAGGGGAC	5	0.125	No Hit
CCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGTCAC	5	0.125	No Hit
AATCCGTGACATCCCAGTCCTTTGAGAAACACCAATGATAAGCCTGTGAG	5	0.125	No Hit
CTCCTGAGTAAAGCGCTTCACCTGTGCCCTATGAGTGAGGGTCAGTAGAC	5	0.125	No Hit
GTAGAGGAGCCATCATGTGACTGGGAAGAGCCCAGTGGGCTCTGGCGGGC	5	0.125	No Hit
TACCTCAGGCACCCAGAAGAGAAGGCCATTCCACAAGGCAGTGTCCGCAA	5	0.125	No Hit
TTCTGGGGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTA	5	0.125	No Hit
TCTGGGGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAGAATCAGACAGTTTTCCC	5	0.125	No Hit
TTTTTTTTTTGGGCTGCTTTTTTGCTGGGCTTTCCACTGCATCCTTGCTA	5	0.125	No Hit
GCGGTTCCGAAAGGGCTGGTCGGCAAGAAAGGCCTGCTAGCCCGCGCCTC	5	0.125	No Hit
CGTGTGCCAACAGGTTCTCTCTGCTCTATGAATGGATTTGCCCTTTTTGC	5	0.125	No Hit
CTGGGTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCAC	5	0.125	No Hit
GGTAAGAGACACACTAGTCGCCCATTTCCCTGTTCACGGATTGCAATCAT	5	0.125	No Hit
GCTTATTTCTTCCCAACCACCACAATTCACAATAGTCTAGAAAAATTTGG	5	0.125	No Hit
CCCCGCAGCATAGCAATCTGGAAAGCTCAGCAAAGGCACAGTGCCCTCGT	5	0.125	No Hit
CGGCTGGGTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.075	0.0	0.0	0.0
5	0.0	0.075	0.0	0.0	0.0
6	0.0	0.075	0.0	0.0	0.0
7	0.0	0.075	0.0	0.0	0.0
8	0.0	0.075	0.0	0.0	0.0
9	0.0	0.075	0.0	0.0	0.0
10-11	0.0	0.075	0.0	0.0	0.0
12-13	0.0	0.1	0.0	0.0	0.0
14-15	0.0	0.16249999999999998	0.0	0.0	0.0
16-17	0.0	0.36250000000000004	0.0	0.0	0.0
18-19	0.0	0.45	0.0	0.0	0.0
20-21	0.0	0.45	0.0	0.0	0.0
22-23	0.0	0.4625	0.0	0.0	0.0
24-25	0.0	0.475	0.0	0.0	0.0
26-27	0.0	0.475	0.0	0.0	0.0
28-29	0.0	0.475	0.0	0.0	0.0
30-31	0.0	0.475	0.0	0.0	0.0
32-33	0.0	0.475	0.0	0.0	0.0
34-35	0.0	0.5125	0.0	0.0	0.0
36-37	0.0	0.5874999999999999	0.0	0.0	0.0
38-39	0.0	0.6	0.0	0.0	0.0
40-41	0.0	0.6	0.0	0.0	0.0
42-43	0.0	0.6	0.0	0.0	0.0
44-45	0.0	0.6	0.0	0.0	0.0
46-47	0.0	0.6	0.0	0.0	0.0
48-49	0.0	0.6	0.0	0.0	0.0
50-51	0.0	0.6	0.0	0.0	0.0
52-53	0.0	0.6	0.0	0.0	0.0
54-55	0.0	0.6	0.0	0.0	0.0
56-57	0.0	0.6	0.0	0.0	0.0
58-59	0.0	0.6	0.0	0.0	0.0
60-61	0.0	0.6	0.0	0.0	0.0
62-63	0.0	0.6	0.0	0.0	0.0
64-65	0.0	0.6	0.0	0.0	0.0
66-67	0.0	0.6	0.0	0.0	0.0
68-69	0.0	0.7625	0.0	0.0	0.0
70-71	0.0	0.9875	0.0	0.0	0.0
72-73	0.0	1.075	0.0	0.0	0.0
74-75	0.0	1.075	0.0	0.0	0.0
76-77	0.0	1.075	0.0	0.0	0.0
78-79	0.0	1.075	0.0	0.0	0.0
80-81	0.0	1.075	0.0	0.0	0.0
82-83	0.0	1.075	0.0	0.0	0.0
84-85	0.0	1.125	0.0	0.0	0.0
86-87	0.0	1.125	0.0	0.0	0.0
88-89	0.0	1.1875	0.0	0.0	0.0
90-91	0.0	1.35	0.0	0.0	0.0
92-93	0.0	1.3875000000000002	0.0	0.0	0.0
94-95	0.0	1.425	0.0	0.0	0.0
96-97	0.0	1.425	0.0	0.0	0.0
98-99	0.0	1.425	0.0	0.0	0.0
100-101	0.0	1.425	0.0	0.0	0.0
102-103	0.0	1.425	0.0	0.0	0.0
104-105	0.0	1.425	0.0	0.0	0.0
106-107	0.0	1.425	0.0	0.0	0.0
108-109	0.0	1.425	0.0	0.0	0.0
110-111	0.0	1.425	0.0	0.0	0.0
112-113	0.0	1.425	0.0	0.0	0.0
114-115	0.0	1.425	0.0	0.0	0.0
116-117	0.0	1.425	0.0	0.0	0.0
118-119	0.0	1.425	0.0	0.0	0.0
120-121	0.0	1.425	0.0	0.0	0.0
122-123	0.0	1.425	0.0	0.0	0.0
124-125	0.0	1.425	0.0	0.0	0.0
126-127	0.0	1.425	0.0	0.0	0.0
128-129	0.0	1.425	0.0	0.0	0.0
130-131	0.0	1.425	0.0	0.0	0.0
132-133	0.0	1.425	0.0	0.0	0.0
134-135	0.0	1.425	0.0	0.0	0.0
136-137	0.0	1.425	0.0	0.0	0.0
138-139	0.0	1.425	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTCTGG	10	0.008971597	132.3625	7
>>END_MODULE
Read 9539723 spots for SRR26281818.sra
Written 9539723 spots for SRR26281818.sra
Read 9539723 spots for SRR26281818.sra
Written 9539723 spots for SRR26281818.sra
Read 9539723 spots for SRR26281818.sra
Written 9539723 spots for SRR26281818.sra
2025-11-10T18:04:40 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
2025-11-10T18:04:45 fastq-dump.3.0.1 sys: path not found while renaming directory within file system module - finalize_v3 - failed to rename the cache-file
Read 9539723 spots for SRR26281818.sra
Written 9539723 spots for SRR26281818.sra
Read 9539723 spots for SRR26281818.sra
Written 9539723 spots for SRR26281818.sra
Read 9539723 spots for SRR26281818.sra
Written 9539723 spots for SRR26281818.sra
Rejected 18311 READS because READLEN < 1
Read 9539725 spots for SRR26281818.sra
Written 9539725 spots for SRR26281818.sra
Read 9539723 spots for SRR26281818.sra
Written 9539723 spots for SRR26281818.sra
SRR ids: ['SRR26281818.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_641pdlkf
SRR26281818.sra spots: 76317786
blocks: [[1, 9539723], [9539724, 19079446], [19079447, 28619169], [28619170, 38158892], [38158893, 47698615], [47698616, 57238338], [57238339, 66778061], [66778062, 76317786]]
SRR26281818 file size 23951736
SRR26281818 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR26281818 SRR26281818_1.fastq SRR26281818_2.fastq
Input file:	SRR26281818_1.fastq
Paired file:	SRR26281818_2.fastq
trimmed:	SRR26281818-trimmed-pair1.fastq, SRR26281818-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Mon Nov 10 18:07:50 2025 >> started

Mon Nov 10 18:11:04 2025 >> done (193.542s)
76302056 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
76302056 (100.00%) read pairs available; of these:
  190263 ( 0.25%) trimmed read pairs available after processing
76111793 (99.75%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 35	    1672	  0.00%
 36	    2029	  0.00%
 37	    2533	  0.00%
 38	    3107	  0.00%
 39	    3891	  0.01%
 40	    4796	  0.01%
 41	    5548	  0.01%
 42	    5989	  0.01%
 43	    5657	  0.01%
 44	    5359	  0.01%
 45	    5341	  0.01%
 46	    5967	  0.01%
 47	    6847	  0.01%
 48	    8574	  0.01%
 49	   10120	  0.01%
 50	   12287	  0.02%
 51	   13633	  0.02%
 52	   13967	  0.02%
 53	   14481	  0.02%
 54	   14035	  0.02%
 55	   14477	  0.02%
 56	   15040	  0.02%
 57	   16409	  0.02%
 58	   18916	  0.02%
 59	   21657	  0.03%
 60	   24446	  0.03%
 61	   27869	  0.04%
 62	   30398	  0.04%
 63	   32935	  0.04%
 64	   34435	  0.05%
 65	   34861	  0.05%
 66	   35582	  0.05%
 67	   36897	  0.05%
 68	   38537	  0.05%
 69	   41383	  0.05%
 70	   45671	  0.06%
 71	   51466	  0.07%
 72	   57837	  0.08%
 73	   65774	  0.09%
 74	   73037	  0.10%
 75	   77819	  0.10%
 76	   81697	  0.11%
 77	   82906	  0.11%
 78	   84165	  0.11%
 79	   86886	  0.11%
 80	   92720	  0.12%
 81	  101959	  0.13%
 82	  113063	  0.15%
 83	  129377	  0.17%
 84	  143843	  0.19%
 85	  156548	  0.21%
 86	  165917	  0.22%
 87	  172184	  0.23%
 88	  175446	  0.23%
 89	  177964	  0.23%
 90	  181683	  0.24%
 91	  192702	  0.25%
 92	  209058	  0.27%
 93	  230711	  0.30%
 94	  254735	  0.33%
 95	  277673	  0.36%
 96	  296963	  0.39%
 97	  310881	  0.41%
 98	  315358	  0.41%
 99	  313615	  0.41%
100	  317076	  0.42%
101	  324656	  0.43%
102	  337157	  0.44%
103	  359247	  0.47%
104	  384987	  0.50%
105	  413664	  0.54%
106	  434428	  0.57%
107	  444641	  0.58%
108	  449456	  0.59%
109	  455398	  0.60%
110	  451866	  0.59%
111	  450623	  0.59%
112	  457078	  0.60%
113	  467376	  0.61%
114	  482939	  0.63%
115	  504799	  0.66%
116	  521154	  0.68%
117	  532207	  0.70%
118	  543200	  0.71%
119	  544987	  0.71%
120	  534904	  0.70%
121	  534233	  0.70%
122	  526912	  0.69%
123	  531132	  0.70%
124	  536346	  0.70%
125	  546487	  0.72%
126	  551041	  0.72%
127	  564247	  0.74%
128	  571937	  0.75%
129	  570887	  0.75%
130	  572247	  0.75%
131	  563686	  0.74%
132	  552534	  0.72%
133	  547781	  0.72%
134	  544115	  0.71%
135	  543115	  0.71%
136	  547112	  0.72%
137	  560255	  0.73%
138	  561636	  0.74%
139	  562575	  0.74%
140	  559935	  0.73%
141	  551756	  0.72%
142	  537584	  0.70%
143	  528276	  0.69%
144	  521124	  0.68%
145	  524454	  0.69%
146	  552886	  0.72%
147	  607735	  0.80%
148	 1168885	  1.53%
149	 5189673	  6.80%
150	21662610	 28.39%
151	18707684	 24.52%
76302056 reads passed initial QC


criterion=sequence-density
sequence-density=0.96
sequence-density-rank=1
fanout-score=2.20
fanout-score-rank=35
prefix-density=0.98
prefix-fanout=2.2
sequence=CGAGACAAGACCAT


criterion=fanout-score
sequence-density=0.04
sequence-density-rank=30
fanout-score=41.61
fanout-score-rank=1
prefix-density=0.91
prefix-fanout=1.7
sequence=CAGAAGCAGCCGCCGGGATGGACGTGTTTCTCATGATCCGGCGCCACAAGACCACCATCTTTACGGACGCCAAGGAGTCGAGCACGGTGTTCGAACTGAAGCGCATCGTCGAGGGCATCCTCAAGCGGCCGCCAGAGGAGCAGCGGCTGTACAAGGACGACCAGCTCCTTGATGATGGAAAAACTCTGGGCGAATGTGGCTTCACCAGTCAGACAGCAAGGCCACAGGCCCCAGCCACAGTGGGCCTGGCCTTTCGAGCAGATGACACCTTTGAAGCGCTGCGTATTGAACCCTTCTCCAGCCCTCCGGAGCTTCCAGATGTGATGAAGCCACAGGATTCTGGAGGCAGTGCCAATGAACAAGCTGTGCAGTGAGGGCCCCAGGCCTACCCCCTAGAAACCCATTCCCCCAATAA


criterion=sequence-density
sequence-density=1.10
sequence-density-rank=1
fanout-score=2.11
fanout-score-rank=34
prefix-density=1.11
prefix-fanout=2.1
sequence=ATGGTCTTGTCTCG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=35
fanout-score=39.93
fanout-score-rank=1
prefix-density=0.12
prefix-fanout=4.3
sequence=AGGGTCCACAGGTCCAGGCTGCAGAGCGGCAGCAGGAAGCAGACCCTCCAATCTAGAACAGGGTAACCTGGTAATAAAATCAGCCCCCTAATGCCGCTCTGACCTCTGAGACGCCACACGCTGCCTACAACAACTAGAGCTCTGGAATAGTAAACAGGAGAGTGATTTCCGGGGGAAATTTTAAATAAGATGCACATGGGACAGGCCATAAAAGTTTGCCAAGTTAAATTTGGTACATAATTGTGTTCACTCCGCGTCCAAACAAAGCGTGTGCGGTCGTCACTCGGCCGCTCCTGCTGCCTCTCAGTATGGCTTGTAGTTATTCTGATGACCACCACGTCGCTGGCTCTTCCCGTAATTTGTACTACCCTGACCTCCACCACTGCCTCGGTTCCCTCGATTGCGATTTCCTCGGCCCCCAGAGCCATACTGCTGTTGCTGGTACACCTCTTTGGGCTGGGCAACCTTGATTTCACACTTGCTACCACTGACAGTATGGAATTTTTTCTCC
SRR26281818 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Nov 10 18:11:29
                             Started mapping on |	Nov 10 18:11:29
                                    Finished on |	Nov 10 18:23:32
       Mapping speed, Million of reads per hour |	379.93

                          Number of input reads |	76302056
                      Average input read length |	275
                                    UNIQUE READS:
                   Uniquely mapped reads number |	70166158
                        Uniquely mapped reads % |	91.96%
                          Average mapped length |	274.29
                       Number of splices: Total |	79766225
            Number of splices: Annotated (sjdb) |	77437372
                       Number of splices: GT/AG |	78749756
                       Number of splices: GC/AG |	546808
                       Number of splices: AT/AC |	52483
               Number of splices: Non-canonical |	417178
                      Mismatch rate per base, % |	0.43%
                         Deletion rate per base |	0.03%
                        Deletion average length |	1.70
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.71
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	6122726
             % of reads mapped to multiple loci |	8.02%
        Number of reads mapped to too many loci |	7221
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.01%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	13198	13198	13198
N_multimapping	6122726	6122726	6122726
N_noFeature	8308760	9254536	68694317
N_ambiguous	1043957	511459	10112
UnstrandedReadsAssigned:60813441 PositiveStrandReadsAssigned:60400163 NegativeStrandReadsAssigned:1461729
Dataset is classified positive stranded
MeadianReadLen=150 20thPercentileLength=122 echo kmer=117
SRR26281818 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 31,715
[index] number of k-mers: 49,915,185
[index] number of equivalence classes: 70,351
[quant] running in paired-end mode
[quant] will process pair 1: SRR26281818-trimmed-pair1.fastq
                             SRR26281818-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 76,302,056 reads, 65,055,732 reads pseudoaligned
[quant] estimated average fragment length: 182.08
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,134 rounds

  31716 SRR26281818.ke.tsv
  32883 SRR26281818.se.tsv
  64599 total
==> SRR26281818.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	773.92	103416	3213.35
ENSRNOT00000040993.4	1039	857.92	153300	4296.97
ENSRNOT00000050156.3	1545	1363.92	989164	17440
ENSRNOT00000043693.3	684	503.337	221667	10590.3
ENSRNOT00000046201.3	204	62.8574	50267	19230.6
ENSRNOT00000046108.3	681	500.337	254785	12245.6
ENSRNOT00000049683.3	784	603.14	326229	13006.8
ENSRNOT00000041241.3	348	178.169	20983	2832.05
ENSRNOT00000044582.3	297	133.655	19234.4	3460.67

==> SRR26281818.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	99
ENSRNOG00000058808	0
ENSRNOG00000061316	3126
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	49
SRR26281818 completed mapping pipeline successfully
