Starting /dee2/code/volunteer_pipeline.sh SRR26281812
    current disk space = 738731168
    free memory = 197717704 
SRR26281812 SRAfilesize
aba12c5526d646c471fd26c32f6f1f3f  SRR26281812.sra
SRR26281812.sra file validated
SRR26281812 is paired end
SRR26281812 is conventional basespace
SRR26281812 read1 length is 51-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR26281812_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51-151
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.291	37.0	37.0	37.0	37.0	37.0
2	36.105	37.0	37.0	37.0	37.0	37.0
3	36.3035	37.0	37.0	37.0	37.0	37.0
4	36.315	37.0	37.0	37.0	37.0	37.0
5	36.371	37.0	37.0	37.0	37.0	37.0
6	36.385	37.0	37.0	37.0	37.0	37.0
7	36.289	37.0	37.0	37.0	37.0	37.0
8	36.33	37.0	37.0	37.0	37.0	37.0
9	36.302	37.0	37.0	37.0	37.0	37.0
10-14	36.3418	37.0	37.0	37.0	37.0	37.0
15-19	36.3412	37.0	37.0	37.0	37.0	37.0
20-24	36.366	37.0	37.0	37.0	37.0	37.0
25-29	36.28059999999999	37.0	37.0	37.0	37.0	37.0
30-34	36.2205	37.0	37.0	37.0	37.0	37.0
35-39	36.2466	37.0	37.0	37.0	37.0	37.0
40-44	36.1659	37.0	37.0	37.0	37.0	37.0
45-49	36.14149999999999	37.0	37.0	37.0	37.0	37.0
50-54	36.11416254063516	37.0	37.0	37.0	37.0	37.0
55-59	36.05647232211375	37.0	37.0	37.0	37.0	37.0
60-64	36.12657313305299	37.0	37.0	37.0	37.0	37.0
65-69	36.04211571752444	37.0	37.0	37.0	37.0	37.0
70-74	36.046163643059685	37.0	37.0	37.0	37.0	37.0
75-79	36.04550597136997	37.0	37.0	37.0	37.0	37.0
80-84	36.0251630328888	37.0	37.0	37.0	37.0	37.0
85-89	35.990927989685545	37.0	37.0	37.0	37.0	37.0
90-94	35.97398834553015	37.0	37.0	37.0	37.0	37.0
95-99	35.96401780708162	37.0	37.0	37.0	37.0	37.0
100-104	35.95869926295502	37.0	37.0	37.0	37.0	37.0
105-109	35.8723987221801	37.0	37.0	37.0	37.0	37.0
110-114	35.866737247483954	37.0	37.0	37.0	37.0	37.0
115-119	35.82079866866667	37.0	37.0	37.0	37.0	37.0
120-124	35.8577164747433	37.0	37.0	37.0	37.0	37.0
125-129	35.679244365660495	37.0	37.0	37.0	37.0	37.0
130-134	35.66404185947884	37.0	37.0	37.0	37.0	37.0
135-139	35.69553176710446	37.0	37.0	37.0	37.0	37.0
140-144	35.61780597002431	37.0	37.0	37.0	37.0	37.0
145-149	35.532126017421405	37.0	37.0	37.0	37.0	37.0
150-151	35.77068177203702	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
26	3.0
27	9.0
28	21.0
29	31.0
30	49.0
31	74.0
32	84.0
33	116.0
34	183.0
35	331.0
36	2548.0
37	551.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.075	24.224999999999998	13.875000000000002	27.825
2	27.24310776942356	20.275689223057643	28.997493734335837	23.483709273182956
3	22.275	25.8	30.375000000000004	21.55
4	20.175	33.85	25.775	20.200000000000003
5	22.225	36.85	23.75	17.175
6	20.7	36.7	22.425	20.175
7	22.125	22.1	35.05	20.724999999999998
8	22.875	24.45	29.049999999999997	23.625
9	22.675	21.3	31.65	24.375
10-14	22.31	27.685	27.485	22.52
15-19	22.56	25.729999999999997	28.34	23.369999999999997
20-24	23.155	27.500000000000004	26.484999999999996	22.86
25-29	21.855	27.700000000000003	27.98	22.465
30-34	22.939999999999998	28.34	27.189999999999998	21.529999999999998
35-39	23.400000000000002	26.924999999999997	27.175	22.5
40-44	22.18	27.255000000000003	29.145	21.42
45-49	23.044999999999998	27.065	27.139999999999997	22.75
50-54	22.30834625193779	29.03435515327299	26.784017602640397	21.87328099214882
55-59	23.005352408583864	26.797058676404383	28.64789155119804	21.549697363813717
60-64	22.2911766177869	27.34097392522897	28.537110254742004	21.830739202242132
65-69	22.677417739269796	29.248259628386837	26.8893674563029	21.184955176040468
70-74	24.435977138273337	28.742605033590696	25.764564323673923	21.056853504462048
75-79	24.302027264952965	28.260978922481012	25.921827053674733	21.51516675889129
80-84	23.05981091056171	28.76788513069417	28.171292785277313	20.00101117346681
85-89	21.82077247977202	28.81787186402728	28.141061523586586	21.220294132614118
90-94	23.286688195191964	27.83330770413655	28.00246040289097	20.877543697780514
95-99	21.94246475576133	29.766425635956928	27.43588409717526	20.855225511106486
100-104	23.371404399323183	29.129653130287647	26.818950930626055	20.67999153976311
105-109	23.329157361512067	28.52551881910779	26.537393104199573	21.607930715180565
110-114	23.509915173304872	28.47031065670468	26.352452109432058	21.667322060558394
115-119	22.40976521434412	28.48966242261418	25.85562434295059	23.244948020091112
120-124	23.45964357399185	27.948421628854692	26.738032966364578	21.85390183078888
125-129	22.675939513813564	28.87768774325273	26.63178714987558	21.814585593058126
130-134	22.79392162090109	28.918954945347906	25.513196480938415	22.773926952812584
135-139	22.82198293944903	28.275765627185006	26.590686617256328	22.311564816109637
140-144	22.225469423540588	28.939869949587198	26.821071089354863	22.013589537517355
145-149	23.62222906403941	27.02432266009852	26.908866995073893	22.444581280788178
150-151	21.740253033823908	13.090627420604184	35.78621223857475	29.382907306997158
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	7.5
26	20.0
27	16.5
28	16.5
29	18.5
30	13.5
31	19.0
32	22.5
33	31.0
34	56.5
35	84.0
36	110.5
37	112.5
38	112.0
39	143.0
40	165.5
41	205.0
42	258.0
43	244.0
44	207.5
45	229.0
46	252.5
47	224.5
48	211.5
49	206.0
50	187.0
51	170.5
52	135.5
53	104.0
54	81.5
55	64.5
56	62.0
57	62.5
58	53.5
59	31.5
60	26.0
61	26.5
62	11.0
63	4.5
64	3.5
65	2.0
66	0.0
67	2.0
68	3.5
69	1.5
70	1.0
71	1.0
72	0.5
73	0.5
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.25
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	1.0
55-59	2.0
60-64	2.0
65-69	2.0
70-74	10.0
75-79	18.0
80-84	23.0
85-89	27.0
90-94	48.0
95-99	59.0
100-104	80.0
105-109	125.0
110-114	113.0
115-119	150.0
120-124	153.0
125-129	136.0
130-134	133.0
135-139	132.0
140-144	116.0
145-149	353.0
150-152	2317.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.75
#Duplication Level	Percentage of deduplicated	Percentage of total
1	37.55395683453238	13.05
2	21.151079136690647	14.7
3	13.81294964028777	14.399999999999999
4	10.287769784172662	14.299999999999999
5	5.683453237410072	9.875
6	3.8129496402877696	7.95
7	2.661870503597122	6.4750000000000005
8	1.223021582733813	3.4000000000000004
9	0.8633093525179856	2.7
>10	2.9496402877697845	13.15
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTGAGTTGCCTCTTTTGCAGACGTCCTAAGGCTAAGCTTGCCTGCTCA	30	0.75	No Hit
CCTAAGGCTAAGCTTGCCTGCTCAGACTAAAGGTTTGCAAGCATAGATGG	24	0.6	No Hit
CTGCCTTGTGGAATGGCCTTCTCTTCTGGGTGCCTGAGGTATCCACTTAT	19	0.475	No Hit
CTTCTCTTCTGGGTGCCTGAGGTATCCACTTATGAGTGGCCTCTCACCGC	18	0.44999999999999996	No Hit
GTCTATTCTTCAGGATGCTCCTGCCTGTTGTTTGCCTCTCTTTAAATTTA	16	0.4	No Hit
GTGGAAAGCCCAGCAAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGC	16	0.4	No Hit
CTGAAGATTCCTTGCCACACTCTACTCTATGTTTATAACCTGCCGGCAAA	15	0.375	No Hit
ACTGAAGATTCCTTGCCACACTCTACTCTATGTTTATAACCTGCCGGCAA	15	0.375	No Hit
GTCAAATTGGCAGCCAACTTTTCTTTTGCACCTGTGACCAAGCTCTAACT	14	0.35000000000000003	No Hit
GCAGGTCATTGTGAGCGATTTCTCACAGGCTTATCATTGGTGTTTCTCAA	14	0.35000000000000003	No Hit
GGGGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCTTCTG	14	0.35000000000000003	No Hit
TTCCAATACCAGTAAAGAAGGGGAACCTGTCCTGTAACTCCTCACCCAGT	13	0.325	No Hit
CTCATGTGTTGCAGATTCTTGGAATGGGCTCCAAGCGTCTACTGACCCTC	13	0.325	No Hit
GCGATTTCTCACAGGCTTATCATTGGTGTTTCTCAAAGGACTGGGATGTC	13	0.325	No Hit
GGGAGACACTTCACAGAAGCAGTCAAAAAAAAGAGAACCTCAGTTCCCAG	12	0.3	No Hit
GTTCATTTCCGATCTACCCCCCAGGTTACCACTGAAGATTCCTTGCCACA	12	0.3	No Hit
AGAAATTCCAATACCAGTAAAGAAGGGGAACCTGTCCTGTAACTCCTCAC	12	0.3	No Hit
CAAAAATAGAGTCAAATTGGCAGCCAACTTTTCTTTTGCACCTGTGACCA	12	0.3	No Hit
CCTCCCAACACTGACCCTTGGCTTCTTCGTTCTAAAAGCCCTGTGAGAAA	12	0.3	No Hit
GGCTAAGCTTGCCTGCTCAGACTAAAGGTTTGCAAGCATAGATGGGACTA	12	0.3	No Hit
ACTCCATTAAGGAATCTGTATTATATTTACCAAATTTTTCTAGACTATTG	11	0.27499999999999997	No Hit
CCTCCCTGGGATCTTCGCCGCCCGAGCTGGACATCATGCCACAGGAAGAG	11	0.27499999999999997	No Hit
GAAAGAACTCCATTAAGGAATCTGTATTATATTTACCAAATTTTTCTAGA	11	0.27499999999999997	No Hit
GATTGAGTGGCAAGAGAGCCATTTTCCGGTTGACAGGAAAGTTCCTGGCT	11	0.27499999999999997	No Hit
GGAGAAACACTGCAGCCCAAAACTTATGGCTACAGTAGTGTGGAGGAGCT	11	0.27499999999999997	No Hit
AGTGGATTGAGTCCCATTGAGCAGCCTCCATCATTTGTTTAAGCAAATGT	11	0.27499999999999997	No Hit
CTCAATTGGAAGCTCTCAGAGCGTGACATGCCCAAGAGGCCAGTCCAATC	11	0.27499999999999997	No Hit
GGCCTATACTAAATATGTAGGAGAAACACTGCAGCCCAAAACTTATGGCT	11	0.27499999999999997	No Hit
CTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCTTCTGCTCCTCC	11	0.27499999999999997	No Hit
CTGAGGTATCCACTTATGAGTGGCCTCTCACCGCTGTTGGAGAGACCTGA	11	0.27499999999999997	No Hit
CATAGATGGGACTATGGACTTTGAAAACACTAGGTTTCACTAAAGTTACT	10	0.25	No Hit
AGAGAATTGTAGTGTTAAAAAATGACATGAAAAACCGGCTAAGTTCATTT	10	0.25	No Hit
GAAAAAACAGAAATTCCAATACCAGTAAAGAAGGGGAACCTGTCCTGTAA	10	0.25	No Hit
CCTGTTGGCACACAGTCTTCCACTAGTCAGATGACTTCTTTCGAGGGAAA	10	0.25	No Hit
GCCTTCTCTTCTGGGTGCCTGAGGTATCCACTTATGAGTGGCCTCTCACC	10	0.25	No Hit
AGAAACCAGAGTTTTATCTGTAATTTGAAGGCCAAGGTTGATCCTTGTTC	10	0.25	No Hit
AGTTATTTCATGCTTCCCTTCTCTGTCTGTCTCCGTTTGATAAGGGTAAG	10	0.25	No Hit
ATGAAAAGAAGTATGGACACAAGTTAAATGTGTCTGATTTATATAAATTA	10	0.25	No Hit
ATGGTGTTGACATCCAGGTCAGCAATGTGGACTACAGGCTGTCCCGGAAG	10	0.25	No Hit
AACAAAAGGGATGGAAAAGGAGACCTCGTTGTTGGCAACCAGTCTAGGCA	10	0.25	No Hit
CCACTTATGAGTGGCCTCTCACCGCTGTTGGAGAGACCTGACATTTACAA	10	0.25	No Hit
AACTTTTTCTTACTTTATTTCTTCCAGTTATTTGAAAGAACTCCATTAAG	9	0.22499999999999998	No Hit
TGGGACTATGGACTTTGAAAACACTAGGTTTCACTAAAGTTACTTGGAGT	9	0.22499999999999998	No Hit
GCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCCCATTTTACCATGG	9	0.22499999999999998	No Hit
GCAATGTGGACTACAGGCTGTCCCGGAAGGAGCTACAGCAGCTCCTCCAA	9	0.22499999999999998	No Hit
GTGATGAGGAACCTTTTCATGAAGAAGCTCTACCTGTGGCCAAGGTTCCA	9	0.22499999999999998	No Hit
CGTCTCCAAGACTATGATGTCAGTTCAGTCTCAGCTTTGACACAGAAGCT	9	0.22499999999999998	No Hit
CCGAGCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCCCATTTTACC	9	0.22499999999999998	No Hit
ATTTAATTAAGAGCTTTCTAGAAAGTTATTTCATGCTTCCCTTCTCTGTC	9	0.22499999999999998	No Hit
CATTTGTATCCGAAAAAGAGAACGAACTCAGGATGAAATAGAAAGGACAA	9	0.22499999999999998	No Hit
ACAAAAGGGATGGAAAAGGAGACCTCGTTGTTGGCAACCAGTCTAGGCAA	9	0.22499999999999998	No Hit
CCCACAGGTGGTCTGGATAAAAGGACATGGTCATAAGAGAATTGTAGTGT	9	0.22499999999999998	No Hit
CCCACGAGGGCACTGTGCCTTTGCTGAGCTTTCCAGATTGCTATGCTGCG	9	0.22499999999999998	No Hit
GGAAATGTTTGTTTCCAACTTTCAGATGAACTTTTTCTTACTTTATTTCT	8	0.2	No Hit
AGCATAGATGGGACTATGGACTTTGAAAACACTAGGTTTCACTAAAGTTA	8	0.2	No Hit
ACTCTCTTTACTAAGTACAGAAACAATGTCTATTCTTCAGGATGCTCCTG	8	0.2	No Hit
AGTGGATTCACAACAAGCCCCCACCTCCCAACACTGACCCTTGGCTTCTT	8	0.2	No Hit
AGGAGAAACACTGCAGCCCAAAACTTATGGCTACAGTAGTGTGGAGGAGC	8	0.2	No Hit
GTTTCAGACTATGGATATTCCAAGCTGATTGAGCTACTAGAAGCCGTGCC	8	0.2	No Hit
CCTGCTCAGACTAAAGGTTTGCAAGCATAGATGGGACTATGGACTTTGAA	8	0.2	No Hit
GGATTTGGAAATCCTTCCCAGTGGGGACCTCACAGAGATTGGTGAGAAGG	8	0.2	No Hit
AGTGTGAGTTGCCTCTTTTGCAGACGTCCTAAGGCTAAGCTTGCCTGCTC	8	0.2	No Hit
ACACTGCCTTGTGGAATGGCCTTCTCTTCTGGGTGCCTGAGGTATCCACT	8	0.2	No Hit
GTTACTGTTCACTGTTGGAAGCATGTTTGGGTTTTTATTATTATGGTAGC	8	0.2	No Hit
ACTATTTGCTTGTCCCAACAAGATGATGACATGGTCATTTGTATCCGAAA	8	0.2	No Hit
CGTCAAAGTAGTGAAGTGGATTCACAACAAGCCCCCACCTCCCAACACTG	8	0.2	No Hit
GGAATCTGTATTATATTTACCAAATTTTTCTAGACTATTGTGAATTGTGG	8	0.2	No Hit
AGAAAATGGATTCTTATTTCATGGCCAGCATTTTGATAAAAATGTCACAA	8	0.2	No Hit
CTCCTCAGAAGCCCCCGGGCTACTGATTAGCAAGGATGCAGTGGAAAGCC	8	0.2	No Hit
GCATAGATGGGACTATGGACTTTGAAAACACTAGGTTTCACTAAAGTTAC	8	0.2	No Hit
GGTTACACCAAAATAACGATGCTAAGCCGTGGCTTTGGAAATTCTCTGGT	7	0.17500000000000002	No Hit
GCAGATTCTTGGAATGGGCTCCAAGCGTCTACTGACCCTCACTCATAGGG	7	0.17500000000000002	No Hit
ATAGTGTCTAGCTTTACTGTTACTGTTCACTGTTGGAAGCATGTTTGGGT	7	0.17500000000000002	No Hit
GAAAAACCGGCTAAGTTCATTTGACCTTTCCCCTGTCAATCATGAAGACC	7	0.17500000000000002	No Hit
TTTTCCGGTTGACAGGAAAGTTCCTGGCTTCTAGGTGACTTATTGGTGTG	7	0.17500000000000002	No Hit
GGCTTCTAAGCAGTGATAAAGTTGCTGTTAGGAGTGTGAGTTGCCTCTTT	7	0.17500000000000002	No Hit
CCCAGCAAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGCCAACTTTT	7	0.17500000000000002	No Hit
ACATTGCCACTGCTCAGAATGGCGTCAAAGTAGTGAAGTGGATTCACAAC	7	0.17500000000000002	No Hit
CCAAGGTTGATCCTTGTTCTTGTGATTTAAAAATTGGCTGTAAATCTGGG	7	0.17500000000000002	No Hit
AGAAGGGGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCT	7	0.17500000000000002	No Hit
GTCAGCAATGTGGACTACAGGCTGTCCCGGAAGGAGCTACAGCAGCTCCT	7	0.17500000000000002	No Hit
CAACTATTTGCTTGTCCCAACAAGATGATGACATGGTCATTTGTATCCGA	7	0.17500000000000002	No Hit
GCTTGCCTGCTCAGACTAAAGGTTTGCAAGCATAGATGGGACTATGGACT	7	0.17500000000000002	No Hit
CAGTGGCCCAACCGCTTCAGTGCCCACACTGAAAAACACAGGTGTGAGGG	7	0.17500000000000002	No Hit
TGTGAATTGTGGTGGTTGGGAAGAAATAAGCAGAGATCGTTCAGCAGGAA	7	0.17500000000000002	No Hit
TGGCGTCAAAGTAGTGAAGTGGATTCACAACAAGCCCCCACCTCCCAACA	7	0.17500000000000002	No Hit
GTGAACATTGCCACTGCTCAGAATGGCGTCAAAGTAGTGAAGTGGATTCA	7	0.17500000000000002	No Hit
CAACACTGACCCTTGGCTTCTTCGTTCTAAAAGCCCTGTGAGAAATCCTC	7	0.17500000000000002	No Hit
GAGAATTGTAGTGTTAAAAAATGACATGAAAAACCGGCTAAGTTCATTTG	7	0.17500000000000002	No Hit
GGTTGATCCTTGTTCTTGTGATTTAAAAATTGGCTGTAAATCTGGGAAGC	7	0.17500000000000002	No Hit
GAAACAATGTCTATTCTTCAGGATGCTCCTGCCTGTTGTTTGCCTCTCTT	7	0.17500000000000002	No Hit
GATAAAAGGACATGGTCATAAGAGAATTGTAGTGTTAAAAAATGACATGA	7	0.17500000000000002	No Hit
TGAAGAGTCTGCCTTCTTTAGTAGAGGTTTTTATGAATGATAAGGCAGAA	7	0.17500000000000002	No Hit
ACTAAAGGTTTGCAAGCATAGATGGGACTATGGACTTTGAAAACACTAGG	7	0.17500000000000002	No Hit
GGTATCCACTTATGAGTGGCCTCTCACCGCTGTTGGAGAGACCTGACATT	7	0.17500000000000002	No Hit
GTGCTTCAAATGGTGTGGGTTCAATATTATTTCCACTAAGAAGAAACCAG	7	0.17500000000000002	No Hit
GGGCAAATCCATTCATAGAGCAGAGAGAACCTGTTGGCACACAGTCTTCC	7	0.17500000000000002	No Hit
AATCTGGGAAGCTCTCAATGTGAAGATGTGTGTGTCCATGTTAGTTCATC	7	0.17500000000000002	No Hit
CTTATGAGTGGCCTCTCACCGCTGTTGGAGAGACCTGACATTTACAATGG	7	0.17500000000000002	No Hit
CACTGCCTAACAGTGGCCCAACCGCTTCAGTGCCCACACTGAAAAACACA	7	0.17500000000000002	No Hit
AGTTGCCTCTTTTGCAGACGTCCTAAGGCTAAGCTTGCCTGCTCAGACTA	7	0.17500000000000002	No Hit
TGAAGATTCCTTGCCACACTCTACTCTATGTTTATAACCTGCCGGCAAAT	7	0.17500000000000002	No Hit
CACTGCAGCCCAAAACTTATGGCTACAGTAGTGTGGAGGAGCTACTGGGA	7	0.17500000000000002	No Hit
ATCCACTTATGAGTGGCCTCTCACCGCTGTTGGAGAGACCTGACATTTAC	7	0.17500000000000002	No Hit
CGCACACTGCTCCAGCTACTGTGGTTCTTGTGTCAACTGATGTTAATTTT	7	0.17500000000000002	No Hit
CACACTGGGGCATGATTGAGTGGCAAGAGAGCCATTTTCCGGTTGACAGG	7	0.17500000000000002	No Hit
CGCTTCAGTGCCCACACTGAAAAACACAGGTGTGAGGGAGACACTTCACA	7	0.17500000000000002	No Hit
CCTATACTAAATATGTAGGAGAAACACTGCAGCCCAAAACTTATGGCTAC	6	0.15	No Hit
CGCAAAGAATGCTGCTGATGATAAACTCCGTCAGAGCCTGCGAAGGTTTG	6	0.15	No Hit
CCTGAGGTATCCACTTATGAGTGGCCTCTCACCGCTGTTGGAGAGACCTG	6	0.15	No Hit
GAGAGAACCTGTTGGCACACAGTCTTCCACTAGTCAGATGACTTCTTTCG	6	0.15	No Hit
ATGGTTTCATGACTTTGACTGAGCTACTGAAGAGTCTGCCTTCTTTAGTA	6	0.15	No Hit
CTTATGGCTACAGTAGTGTGGAGGAGCTACTGGGAGCCATCCCACAGGTG	6	0.15	No Hit
TCCCAACACTGACCCTTGGCTTCTTCGTTCTAAAAGCCCTGTGAGAAATC	6	0.15	No Hit
CCAAGCTCTAACTTCCAGTTTGAACACAGAATTAAATTAGGATGGGGGGA	6	0.15	No Hit
ATTCAAGAGCTGAATAATTGTCAGGTAACTGTTGCTCACATAAACGCTAC	6	0.15	No Hit
CTTTGACACAGAAGCTCTGCCATGTTGTAAAGGTTGCTGATATGGAGTCT	6	0.15	No Hit
GAGATCGTTCAGCAGGAAGTGGATTGAGTCCCATTGAGCAGCCTCCATCA	6	0.15	No Hit
GAAATAAGCAGAGATCGTTCAGCAGGAAGTGGATTGAGTCCCATTGAGCA	6	0.15	No Hit
CTGTTGGGCAAAAAGGGCAAATCCATTCATAGAGCAGAGAGAACCTGTTG	6	0.15	No Hit
CTATTCTTCAGGATGCTCCTGCCTGTTGTTTGCCTCTCTTTAAATTTATA	6	0.15	No Hit
ACTGCTCAGAATGGCGTCAAAGTAGTGAAGTGGATTCACAACAAGCCCCC	6	0.15	No Hit
CTGAAGCCTTGTTACATCATGCTAACCAGCTGATCAGATTTGAAGAGTTC	6	0.15	No Hit
ATATCATCCTGAGTGAGGTAACCCAGTCACAAAAGAACACACATGCTGTG	6	0.15	No Hit
AAACCGGCTAAGTTCATTTGACCTTTCCCCTGTCAATCATGAAGACCAGC	6	0.15	No Hit
GTTGCCTCTTTTGCAGACGTCCTAAGGCTAAGCTTGCCTGCTCAGACTAA	6	0.15	No Hit
GGACTACAGGCTGTCCCGGAAGGAGCTACAGCAGCTCCTCCAAGAAGCCT	6	0.15	No Hit
GGTCATTTGTATCCGAAAAAGAGAACGAACTCAGGATGAAATAGAAAGGA	6	0.15	No Hit
GGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCTTCTGCT	6	0.15	No Hit
AGCAGAGATCGTTCAGCAGGAAGTGGATTGAGTCCCATTGAGCAGCCTCC	6	0.15	No Hit
GCTACTAGAAGCCGTGCCTCATGTGTTGCAGATTCTTGGAATGGGCTCCA	6	0.15	No Hit
GTCTAGGCAAAGCTGCTTCTAAATTTGGGTCACCAGAAGTTGCAGTCACT	6	0.15	No Hit
GGATATTCCAAGCTGATTGAGCTACTAGAAGCCGTGCCTCATGTGTTGCA	6	0.15	No Hit
TGCTAGCCCGCGCCTCCCTGGGATCTTCGCCGCCCGAGCTGGACATCATG	6	0.15	No Hit
GGCAAATTCTGGATCTGCTACAAAAAACACAAACTTTAAAGGTTTACAGG	6	0.15	No Hit
CCTCCATCATTTGTTTAAGCAAATGTATTTGTGGTTGTTCAAGAAGCCAA	6	0.15	No Hit
AAGGAATCTGTATTATATTTACCAAATTTTTCTAGACTATTGTGAATTGT	6	0.15	No Hit
CTTCGCCGCCCGAGCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCC	6	0.15	No Hit
ACTTGATTGATATAATATCAGAGATCCCAGACACAACTATTTGCTTGTCC	6	0.15	No Hit
TCAGTCTTGGTCTCCGAGGAGTATGTCTCCAAACCTTTTGAACACCGCCT	6	0.15	No Hit
GCTACTGGGAGCCATCCCACAGGTGGTCTGGATAAAAGGACATGGTCATA	6	0.15	No Hit
GCTGTGGAACAAAAGGGATGGAAAAGGAGACCTCGTTGTTGGCAACCAGT	6	0.15	No Hit
GCCAACTTTTCTTTTGCACCTGTGACCAAGCTCTAACTTCCAGTTTGAAC	6	0.15	No Hit
TTCCTTGCCACACTCTACTCTATGTTTATAACCTGCCGGCAAATAAAGAT	6	0.15	No Hit
GTCTCCTGCAGACCCACGAGGGCACTGTGCCTTTGCTGAGCTTTCCAGAT	6	0.15	No Hit
GGGAGCCTGGCCACACTGCCTAACAGTGGCCCAACCGCTTCAGTGCCCAC	6	0.15	No Hit
TGGAAAGCCCAGCAAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGCC	6	0.15	No Hit
CTACTGGGAGCCATCCCACAGGTGGTCTGGATAAAAGGACATGGTCATAA	6	0.15	No Hit
GGAAAGTTCCTGGCTTCTAGGTGACTTATTGGTGTGCCTTGTTTTTTATG	6	0.15	No Hit
GTGAGTTGCCTCTTTTGCAGACGTCCTAAGGCTAAGCTTGCCTGCTCAGA	6	0.15	No Hit
GGAAGAGGAGGCAACAGGGTTGCTGCACCCATGAGAAGGTATGAAGGAAC	6	0.15	No Hit
GTTCACTGTTGGAAGCATGTTTGGGTTTTTATTATTATGGTAGCTGATGT	6	0.15	No Hit
GACGTCCTAAGGCTAAGCTTGCCTGCTCAGACTAAAGGTTTGCAAGCATA	6	0.15	No Hit
GTTGGGAAGAAATAAGCAGAGATCGTTCAGCAGGAAGTGGATTGAGTCCC	6	0.15	No Hit
AGACACAACTATTTGCTTGTCCCAACAAGATGATGACATGGTCATTTGTA	6	0.15	No Hit
GTGAAGCGCTTTACTCAGGATTTACTGAAACTTCTCAAGTCCCAGGCCAG	6	0.15	No Hit
AAAGCCCAGCAAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGCCAAC	6	0.15	No Hit
CAGACACAACTATTTGCTTGTCCCAACAAGATGATGACATGGTCATTTGT	6	0.15	No Hit
GCCTTCTCCAAGCATGGCCAGGTGAAGAGTGTTGAGCTCAGTCCCCATAC	6	0.15	No Hit
ACCCAAAAGATGCTCCAACATATAACAAGGACACATGCTCCACTGTGTTC	6	0.15	No Hit
GTCAAAAAAAAGAGAACCTCAGTTCCCAGAGCATTGCCAGCTCTCCTGCA	5	0.125	No Hit
ATAGATGGGACTATGGACTTTGAAAACACTAGGTTTCACTAAAGTTACTT	5	0.125	No Hit
GCCAACAAGTCACTCTCTTTACTAAGTACAGAAACAATGTCTATTCTTCA	5	0.125	No Hit
GCTGATGATAAACTCCGTCAGAGCCTGCGAAGGTTTGCAAATACGCACAC	5	0.125	No Hit
GCTACAGTAGTGTGGAGGAGCTACTGGGAGCCATCCCACAGGTGGTCTGG	5	0.125	No Hit
CTTGACGTCTTTAATAGTGTCTAGCTTTACTGTTACTGTTCACTGTTGGA	5	0.125	No Hit
CCCTGGGATCTTCGCCGCCCGAGCTGGACATCATGCCACAGGAAGAGTGA	5	0.125	No Hit
TGTCAGTTCAGTCTCAGCTTTGACACAGAAGCTCTGCCATGTTGTAAAGG	5	0.125	No Hit
TGAAAAACCGGCTAAGTTCATTTGACCTTTCCCCTGTCAATCATGAAGAC	5	0.125	No Hit
GAAGCCCCCGGGCTACTGATTAGCAAGGATGCAGTGGAAAGCCCAGCAAA	5	0.125	No Hit
TTGGGAGACTTTGCCTCATCAAAGACACAGGTGAACAGTCACCCAGTGCC	5	0.125	No Hit
TGCAAGCATAGATGGGACTATGGACTTCGAAAACACTAGGTTTCACTAAA	5	0.125	No Hit
TTTAAGGAAAAGAGATGGTCTCGATGTCATCCATTTAAGTCAGCATTTGA	5	0.125	No Hit
AGTGCTTCAAATGGTGTGGGTTCAATATTATTTCCACTAAGAAGAAACCA	5	0.125	No Hit
GTTATTTCATGCTTCCCTTCTCTGTCTGTCTCCGTTTGATAAGGGTAAGG	5	0.125	No Hit
GTCACACACTGGGGCATGATTGAGTGGCAAGAGAGCCATTTTCCGGTTGA	5	0.125	No Hit
TGCTGTTAGGAGTGTGAGTTGCCTCTTTTGCAGACGTCCTAAGGCTAAGC	5	0.125	No Hit
AGGGCACTGTGCCTTTGCTGAGCTTTCCAGATTGCTATGCTGCGGAGTTT	5	0.125	No Hit
GGCCAGCAAGCAGGTCATTGTGAGCGATTTCTCACAGGCTTATCATTGGT	5	0.125	No Hit
GCCTTGTGGAATGGCCTTCTCTTCTGGGTGCCTGAGGTATCCACTTATGA	5	0.125	No Hit
CACTCACAGTACTCCTCTCAACCCCTGTGAATATGGTTTCATGACTTTGA	5	0.125	No Hit
ACTGATGTTAATTTTGCATTAGAACTCAGTGATCTCAGGCACCGGCATGG	5	0.125	No Hit
GCTGCTTCTAAATTTGGGTCACCAGAAGTTGCAGTCACTGGACAGGTGCT	5	0.125	No Hit
CCAAAATAACGATGCTAAGCCGTGGCTTTGGAAATTCTCTGGTTGCTTTT	5	0.125	No Hit
AGCTACTGGGAGCCATCCCACAGGTGGTCTGGATAAAAGGACATGGTCAT	5	0.125	No Hit
AGAAGCAGAGTTTATCTGTGTGTGTGACATCAGTAAAGAGAACAAGGAAG	5	0.125	No Hit
GCAAGTCAGTCCTCTGTTCAGCTCTCAGTCTTGGTCTCCGAGGAGTATGT	5	0.125	No Hit
AGTGGCCCAACCGCTTCAGTGCCCACACTGAAAAACACAGGTGTGAGGGA	5	0.125	No Hit
TTTGCGGACACTGCCTTGTGGAATGGCCTTCTCTTCTGGGTGCCTGAGGT	5	0.125	No Hit
GCAGCACTGCTCGAACAAGGACTTCAGCGAACTTGTATTTGATCCAGACT	5	0.125	No Hit
TGTGGAATGGCCTTCTCTTCTGGGTGCCTGAGGTATCCACTTATGAGTGG	5	0.125	No Hit
GGTGAAGCGCTTTACTCAGGATTTACTGAAACTTCTCAAGTCCCAGGCCA	5	0.125	No Hit
GTGAGCCTGGCTCGGGCTGTGTATTGTAACTCTGACATCTACCTCTTGGA	5	0.125	No Hit
ATGGAGATAATGAGACACTTTTTCTGGGTGGAAGGATGTACCCAGTTAGA	5	0.125	No Hit
GTATCCACTTATGAGTGGCCTCTCACCGCTGTTGGAGAGACCTGACATTT	5	0.125	No Hit
TATGGTTTCATGACTTTGACTGAGCTACTGAAGAGTCTGCCTTCTTTAGT	5	0.125	No Hit
GGAAAGCCCAGCAAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGCCA	5	0.125	No Hit
AGACTGTCTAAACAAGCCAGCAAGAAATAGTATAATTGATGCTGCTAAAA	5	0.125	No Hit
CGCTTTTTAAAACAGGCTCTCAAAAAAAATACTGTATTTCTTTGAAAGTA	5	0.125	No Hit
CCTTGCCACACTCTACTCTATGTTTATAACCTGCCGGCAAATAAAGATGG	5	0.125	No Hit
CTCTCAGTCTTGGTCTCCGAGGAGTATGTCTCCAAACCTTTTGAACACCG	5	0.125	No Hit
AGAGAAGCAGAGTTTATCTGTGTGTGTGACATCAGTAAAGAGAACAAGGA	5	0.125	No Hit
CTGGGAGCCATCCCACAGGTGGTCTGGATAAAAGGACATGGTCATAAGAG	5	0.125	No Hit
TAATGGTCCATAGGGAAATGTTTGTTTCCAACTTTCAGATGAACTTTTTC	5	0.125	No Hit
ACTGAAACTTCTCAAGTCCCAGGCCAGCAAGCAGGTCATTGTGAGCGATT	5	0.125	No Hit
GTTAACCAATGTGGCCTCATCCCATTTTTTAAAAAAGTTTCCATTTGGGG	5	0.125	No Hit
CCTGGGATCTTCGCCGCCCGAGCTGGACATCATGCCACAGGAAGAGTGAG	5	0.125	No Hit
AATACGCACACTGCTCCAGCTACTGTGGTTCTTGTGTCAACTGATGTTAA	5	0.125	No Hit
CTGGGATCTTCGCCGCCCGAGCTGGACATCATGCCACAGGAAGAGTGAGC	5	0.125	No Hit
TTGAAAGAACTCCATTAAGGAATCTGTATTATATTTACCAAATTTTTCTA	5	0.125	No Hit
TATTTTTTCTAGAAAGCCAGAAAATGGATTCTTATTTCATGGCCAGCATT	5	0.125	No Hit
AGGTCATTGTGAGCGATTTCTCACAGGCTTATCATTGGTGTTTCTCAAAG	5	0.125	No Hit
CTTTAATAGTGTCTAGCTTTACTGTTACTGTTCACTGTTGGAAGCATGTT	5	0.125	No Hit
CCAGCATCTGCTTCTGCTCCTCCCAAACCCTCCTCAGAAGCCCCCGGGCT	5	0.125	No Hit
GACATGGTCATTTGTATCCGAAAAAGAGAACGAACTCAGGATGAAATAGA	5	0.125	No Hit
GAGCTTTCCAGATTGCTATGCTGCGGAGTTTGGTGACCTAGAAATTACGC	5	0.125	No Hit
CACTGCCTTGTGGAATGGCCTTCTCTTCTGGGTGCCTGAGGTATCCACTT	5	0.125	No Hit
GGTCATAAGAGAATTGTAGTGTTAAAAAATGACATGAAAAACCGGCTAAG	5	0.125	No Hit
CTTCAAATGGTGTGGGTTCAATATTATTTCCACTAAGAAGAAACCAGAGT	5	0.125	No Hit
GTTTGATAAGGGTAAGGTTTAAGGAAAAGAGATGGTCTCGATGTCATCCA	5	0.125	No Hit
GCCTATACTAAATATGTAGGAGAAACACTGCAGCCCAAAACTTATGGCTA	5	0.125	No Hit
TGAAAAGAAGTATGGACACAAGTTAAATGTGTCTGATTTATATAAATTAA	5	0.125	No Hit
CCAACTCTCTCCACCTAAATGTGGCACCTCCGGTTTGTTTAAAAGGCTCA	5	0.125	No Hit
AAGCATGTTTGGGTTTTTATTATTATGGTAGCTGATGTTGGCATTTGGGA	5	0.125	No Hit
AGATCCCAGACACAACTATTTGCTTGTCCCAACAAGATGATGACATGGTC	5	0.125	No Hit
CTACAGGCTGTCCCGGAAGGAGCTACAGCAGCTCCTCCAAGAAGCCTTCT	5	0.125	No Hit
AGCCCAGCAAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGCCAACTT	5	0.125	No Hit
AATCCATTCATAGAGCAGAGAGAACCTGTTGGCACACAGTCTTCCACTAG	5	0.125	No Hit
TGTAAATCTGGGAAGCTCTCAATGTGAAGATGTGTGTGTCCATGTTAGTT	5	0.125	No Hit
TGATAAACTCCGTCAGAGCCTGCGAAGGTTTGCAAATACGCACACTGCTC	5	0.125	No Hit
GCCCAGCAAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGCCAACTTT	5	0.125	No Hit
ATGGCCTTCTCTTCTGGGTGCCTGAGGTATCCACTTATGAGTGGCCTCTC	5	0.125	No Hit
GGCAAAGCTGCTTCTAAATTTGGGTCACCAGAAGTTGCAGTCACTGGACA	5	0.125	No Hit
AAGCAGTCAAAAAAAAGAGAACCTCAGTTCCCAGAGCATTGCCAGCTCTC	5	0.125	No Hit
AGTGAACATTGCCACTGCTCAGAATGGCGTCAAAGTAGTGAAGTGGATTC	5	0.125	No Hit
GAAGAAAAAACAGAAATTCCAATACCAGTAAAGAAGGGGAACCTGTCCTG	5	0.125	No Hit
CCGTTTGATAAGGGTAAGGTTTAAGGAAAAGAGATGGTCTCGATGTCATC	5	0.125	No Hit
GCTCAGACTAAAGGTTTGCAAGCATAGATGGGACTATGGACTTTGAAAAC	5	0.125	No Hit
TGAAGTGCTTCAAATGGTGTGGGTTCAATATTATTTCCACTAAGAAGAAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138-139	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR26281812 read2 length is 51-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR26281812_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51-151
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.1125	37.0	37.0	37.0	37.0	37.0
2	36.228	37.0	37.0	37.0	37.0	37.0
3	36.338	37.0	37.0	37.0	37.0	37.0
4	36.367	37.0	37.0	37.0	37.0	37.0
5	36.2615	37.0	37.0	37.0	37.0	37.0
6	36.285	37.0	37.0	37.0	37.0	37.0
7	36.1305	37.0	37.0	37.0	37.0	37.0
8	36.272	37.0	37.0	37.0	37.0	37.0
9	36.156	37.0	37.0	37.0	37.0	37.0
10-14	36.278999999999996	37.0	37.0	37.0	37.0	37.0
15-19	36.2683	37.0	37.0	37.0	37.0	37.0
20-24	36.2323	37.0	37.0	37.0	37.0	37.0
25-29	36.2106	37.0	37.0	37.0	37.0	37.0
30-34	36.1445	37.0	37.0	37.0	37.0	37.0
35-39	36.1353	37.0	37.0	37.0	37.0	37.0
40-44	36.16180000000001	37.0	37.0	37.0	37.0	37.0
45-49	36.1072	37.0	37.0	37.0	37.0	37.0
50-54	36.12686704176044	37.0	37.0	37.0	37.0	37.0
55-59	36.121606494479906	37.0	37.0	37.0	37.0	37.0
60-64	35.977676724928486	37.0	37.0	37.0	37.0	37.0
65-69	35.938114536546834	37.0	37.0	37.0	37.0	37.0
70-74	35.98055238466027	37.0	37.0	37.0	37.0	37.0
75-79	35.97801281850941	37.0	37.0	37.0	37.0	37.0
80-84	35.92714733654812	37.0	37.0	37.0	37.0	37.0
85-89	35.92671978707972	37.0	37.0	37.0	37.0	37.0
90-94	35.86378197711798	37.0	37.0	37.0	37.0	37.0
95-99	35.82838163959793	37.0	37.0	37.0	37.0	37.0
100-104	35.781141983287846	37.0	37.0	37.0	37.0	37.0
105-109	35.79016737599221	37.0	37.0	37.0	37.0	37.0
110-114	35.68426100716448	37.0	37.0	37.0	37.0	37.0
115-119	35.746481831509584	37.0	37.0	37.0	37.0	37.0
120-124	35.74137444326086	37.0	37.0	37.0	37.0	37.0
125-129	35.69529993114067	37.0	37.0	37.0	37.0	37.0
130-134	35.54688672171644	37.0	37.0	37.0	37.0	37.0
135-139	35.38236162009267	37.0	37.0	37.0	32.2	37.0
140-144	35.35963301538861	37.0	37.0	37.0	32.2	37.0
145-149	35.16070931033078	37.0	37.0	37.0	27.4	37.0
150-151	35.644957640925384	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	1.0
24	5.0
25	3.0
26	6.0
27	12.0
28	21.0
29	24.0
30	49.0
31	68.0
32	68.0
33	119.0
34	170.0
35	455.0
36	2638.0
37	360.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.574999999999996	8.225	14.85	44.35
2	23.45	6.875000000000001	32.074999999999996	37.6
3	23.849999999999998	10.4	23.35	42.4
4	25.324999999999996	14.825	17.474999999999998	42.375
5	25.85	23.45	23.575	27.125
6	29.049999999999997	25.05	22.6	23.3
7	17.775	31.3	36.625	14.299999999999999
8	23.175	25.674999999999997	31.424999999999997	19.725
9	20.4	25.7	29.2	24.7
10-14	22.485	30.209999999999997	27.045	20.26
15-19	20.75	28.175	28.49	22.585
20-24	21.245	24.845	31.490000000000002	22.42
25-29	23.925	28.07	27.525	20.48
30-34	21.37	27.875	27.665	23.09
35-39	22.564999999999998	24.925	31.324999999999996	21.185000000000002
40-44	22.17	27.279999999999998	30.380000000000003	20.169999999999998
45-49	22.455	26.815	27.439999999999998	23.29
50-54	22.01830274541181	28.349252387858183	25.048757313597044	24.58368755313297
55-59	20.724325946676004	27.497373818218197	27.292281526687006	24.48601870841879
60-64	21.393532886174793	27.31504655120633	28.59145059565522	22.69996996696366
65-69	22.766671676937722	27.666716769377224	28.28298010922391	21.283631444461147
70-74	22.276284109149277	25.82263242375602	26.926163723916535	24.97491974317817
75-79	21.485731541597463	25.693291056419547	30.353817504655495	22.467159897327495
80-84	20.153783893160664	27.848037231889922	29.421286928369085	22.576891946580332
85-89	21.75131170088126	28.38367887524833	28.73006978758087	21.134939636289545
90-94	20.57570937451896	30.791728667453434	27.235876648365743	21.396685309661862
95-99	23.803572358485653	28.714263396344318	25.94386293808259	21.538301307087433
100-104	22.455676104789628	25.938078856840434	31.08229690394284	20.523948134427098
105-109	22.64922322158626	28.814390842191333	28.18206595802671	20.354319978195694
110-114	23.293059848271984	26.080359651587525	27.569542006181514	23.057038493958977
115-119	22.091732398480865	25.054046158340636	27.297692082968155	25.55652936021034
120-124	20.56103200681514	28.380187416331992	27.41876597298284	23.640014603870025
125-129	23.083796030378455	23.696470738400663	29.29350947731189	23.926223753908992
130-134	21.200612965554	26.59071223932307	29.982010793523884	22.22666400159904
135-139	18.57492141110723	27.188264058679707	29.123297240656655	25.11351728955641
140-144	18.072992700729927	29.255474452554747	29.44525547445255	23.226277372262775
145-149	21.317650676506766	30.55811808118081	25.44587945879459	22.678351783517837
150-151	22.09175314036046	14.11796832332059	37.083560895685416	26.70671764063353
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	4.0
25	5.0
26	4.0
27	5.0
28	5.0
29	10.0
30	17.0
31	24.5
32	32.0
33	39.0
34	57.0
35	79.0
36	92.0
37	118.5
38	144.5
39	139.5
40	139.5
41	160.0
42	189.0
43	204.0
44	222.5
45	319.5
46	325.5
47	201.0
48	148.0
49	160.5
50	181.0
51	186.0
52	156.0
53	114.0
54	84.0
55	71.0
56	106.0
57	126.0
58	79.0
59	35.5
60	17.0
61	7.5
62	3.5
63	1.5
64	0.5
65	0.0
66	0.5
67	4.0
68	4.5
69	1.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	1.0
55-59	2.0
60-64	3.0
65-69	3.0
70-74	10.0
75-79	18.0
80-84	25.0
85-89	27.0
90-94	48.0
95-99	59.0
100-104	79.0
105-109	124.0
110-114	112.0
115-119	151.0
120-124	153.0
125-129	133.0
130-134	133.0
135-139	129.0
140-144	118.0
145-149	440.0
150-152	2232.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.9
#Duplication Level	Percentage of deduplicated	Percentage of total
1	46.17414248021108	17.5
2	19.92084432717678	15.1
3	13.720316622691293	15.6
4	8.443271767810026	12.8
5	3.95778364116095	7.5
6	2.044854881266491	4.65
7	1.517150395778364	4.025
8	1.3192612137203166	4.0
9	0.7255936675461742	2.475
>10	2.044854881266491	13.25
>50	0.13192612137203166	3.1
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCCCAGATCACACACCCATTCACTGTACACAACTCTTCTTTGTTGCCCAC	65	1.625	No Hit
CCTCGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTTCT	59	1.4749999999999999	No Hit
CTCGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTTCTC	48	1.2	No Hit
CTCCCCAGATCACACACCCATTCACTGTACACAACTCTTCTTTGTTGCCC	43	1.075	No Hit
GTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACCTCC	40	1.0	No Hit
CCCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGTC	39	0.975	No Hit
CCCCAGTGTGTGACCCACAGGTTTAAGGACATGTTTGGCTTCTTGAACAA	36	0.8999999999999999	No Hit
CCCAGATCACACACCCATTCACTGTACACAACTCTTCTTTGTTGCCCACA	23	0.575	No Hit
GGGGGTAGATCGGAAATGAACTCTTCAAATCTGATCAGCTGGTTAGCATG	16	0.4	No Hit
CCTCTGGATAAAGAAATAGTGATTGGACTGGCCTCTTGGGCATGTCACGC	15	0.375	No Hit
CCCGGCACTGCTTTCCAAAATGGTGATGGTACAAAGGGATGAAATTACTG	14	0.35000000000000003	No Hit
CCAGATCACACACCCATTCACTGTACACAACTCTTCTTTGTTGCCCACAG	14	0.35000000000000003	No Hit
GGGGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAGAAT	14	0.35000000000000003	No Hit
GGGCTGCTTTTTTGCTGGGCTTTCCACTGCATCCTTGCTAATCAGTAGCC	14	0.35000000000000003	No Hit
CTGCTCAATGGGACTCAATCCACTTCCTGCTGAACGATCTCTGCTTATTT	13	0.325	No Hit
GCCGGTTTTTCATGTCATTTTTTAACACTACAATTCTCTTATGACCATGT	13	0.325	No Hit
GTTCTTTAGTAAATCAATGACTTCTCTGCTGAACTGAATCAGCTGAGGAT	13	0.325	No Hit
CCCCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGT	13	0.325	No Hit
CTGCCGGCCAAAGTGGTGGTGGTAAGAGGGAATGAACTTGTTAAAGGGCA	13	0.325	No Hit
GCCGGCCAAAGTGGTGGTGGTAAGAGGGAATGAACTTGTTAAAGGGCATT	12	0.3	No Hit
CCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGTCA	12	0.3	No Hit
GTCCGCAAAGGCTAGCTTTTCCCAAATGCCAACATCAGCTACCATAATAA	11	0.27499999999999997	No Hit
GCTGCTTTTTTGCTGGGCTTTCCACTGCATCCTTGCTAATCAGTAGCCCG	11	0.27499999999999997	No Hit
GTTGAGAGGAGTACTGTGAGTGGTTTCATAATGTCTCTTGAGCTCATCAA	11	0.27499999999999997	No Hit
GGGGCAGGTCATAGTGCTGAGAACTGGGAATCACTGGCTTCATTTAAAAG	11	0.27499999999999997	No Hit
GTCTTGTTCTTCAGTAGGCCCATGGGACCCACCACCTTCTCAAAGATGTG	11	0.27499999999999997	No Hit
GGCTGGTCTTCATGATTGACAGGGGAAAGGTCAAATGAACTTAGCCGGTT	10	0.25	No Hit
CCCGGTTTCTGGTTTCAGCTTGCTGCAGCTGAGTCTCCAATTCAGCTTCA	10	0.25	No Hit
TCACCACTCAGCTGATTTACAATTTATGCATTTAATGCATAGTTTAAAAA	10	0.25	No Hit
CACCCTTATCAAACGGAGACAGACAGAGAAGGGAAGCATGAAATAACTTT	10	0.25	No Hit
GTGCCAACAGGTTCTCTCTGCTCTATGAATGGATTTGCCCTTTTTGCCCA	10	0.25	No Hit
GCTGCAGTTTGTAGAGGAGCCATCATGTGACTGGGAAGAGCCCAGTGGGC	10	0.25	No Hit
GGGCTGGTGGCGCAGCAAGTCCACAACATCTTTGGAGAACTGCTTTGTCC	10	0.25	No Hit
TGTCCTTTTATCCAGACCACCTGTGGGATGGCTCCCAGTAGCTCCTCCAC	9	0.22499999999999998	No Hit
GTAGAATCAGACAGTTTTCCCCCCATCCTAATTTAATTCTGTGTTCAAAC	9	0.22499999999999998	No Hit
CGGCAGGTTATAAACATAGAGTAGAGTGTGGCAAGGAATCTTCAGTGGTA	9	0.22499999999999998	No Hit
CTCTCCAATAGTTTGTTTCTTCCTCCTCTGATCCAAAAGTCTTTCCTGAG	9	0.22499999999999998	No Hit
CGGTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACCT	9	0.22499999999999998	No Hit
CTACCATAATAATAAAAACCCAAACATGCTTCCAACAGTGAACAGTAACA	9	0.22499999999999998	No Hit
CCTGGTTACTGGGTAAGAGACACACTAGTCGCCCATTTCCCTGTTCACGG	9	0.22499999999999998	No Hit
GCCCGCTCTGCGCTGTCTTGGTTTATAAAGCGGAGAATAGCGCTGCAGCC	9	0.22499999999999998	No Hit
CACCCATTCACTGTACACAACTCTTCTTTGTTGCCCACAGAACCTGACCT	9	0.22499999999999998	No Hit
TCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACCTCCA	9	0.22499999999999998	No Hit
CCTCTCAATACACAATATGTCATTCAAATGCTGACTTAAATGGATGACAT	9	0.22499999999999998	No Hit
GGCTGGTGGCGCAGCAAGTCCACAACATCTTTGGAGAACTGCTTTGTCCT	8	0.2	No Hit
CCCAAACATGCTTCCAACAGTGAACAGTAACAGTAAAGCTAGACACTATT	8	0.2	No Hit
CACCACTCAGCTGATTTACAATTTATGCATTTAATGCATAGTTTAAAAAA	8	0.2	No Hit
GCGGTGTTCAAAAGGTTTGGAGACATACTCCTCGGAGACCAAGACTGAGA	8	0.2	No Hit
CTCCAGGATCTGCCTGCTGACTGCAGGCTGGTCTTCATGATTGACAGGGG	8	0.2	No Hit
GTGTGTGACCCACAGGTTTAAGGACATGTTTGGCTTCTTGAACAACCACA	8	0.2	No Hit
GGGCATTCGGAAATGGGGCTGGTGGCGCAGCAAGTCCACAACATCTTTGG	8	0.2	No Hit
GTGCAGGAGAGCTGGCAATGCTCTGGGAACTGAGGTTCTCTTTTTTTTGA	8	0.2	No Hit
CCTGAGTTCGTTCTCTTTTTCGGATACAAATGACCATGTCATCATCTTGT	8	0.2	No Hit
GCCAACAGGTTCTCTCTGCTCTATGAATGGATTTGCCCTTTTTGCCCAAC	8	0.2	No Hit
CTCCCAAGTCTCATCAAGAGCAAACAGTGTAGGATGGGAGGATGGGAAAG	8	0.2	No Hit
CCCCTGTTCACGGATTGCAATCATGTCTGTTAATTTATATAAATCAGACA	8	0.2	No Hit
GGGGTTCTGGCACCTCCAGGATCTGCCTGCTGACTGCAGGCTGGTCTTCA	8	0.2	No Hit
TGCAAAAGAGGCAACTCACACTCCTAACAGCAACTTTATCACTGCTTAGA	8	0.2	No Hit
CCAGAAAACACCAATGGGGGGGAGGGTCTCCAGCACCTGTCCAGTGACTG	8	0.2	No Hit
ACCGTATTCCGTGACATCCCAGTCCTTTGAGAAACACCAATGATAAGCCT	8	0.2	No Hit
CGTCCTTTCTATTTCATCCTGAGTTCGTTCTCTTTTTCGGATACAAATGA	8	0.2	No Hit
CGGTTTTTCATGTCATTTTTTAACACTACAATTCTCTTATGACCATGTCC	8	0.2	No Hit
CTGGCTGGGTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTG	8	0.2	No Hit
GTTGGAAACAAACATTTCCCTATGGACCATTAATAAAAGTGGCTTCTGGG	8	0.2	No Hit
CTCTCAATACACAATATGTCATTCAAATGCTGACTTAAATGGATGACATC	7	0.17500000000000002	No Hit
CTCTGATCCAAAAGTCTTTCCTGAGTCCTCCATGGTAAAATGGGCAAGAG	7	0.17500000000000002	No Hit
CCTGTGGAGAGTGACACCAGTATCTTCTTGCTGCCAATTTTGTATCTGTG	7	0.17500000000000002	No Hit
CTGCAGAACATTTCTAGAGCAGCTCCATCTGGGGCAGGTCATAGTGCTGA	7	0.17500000000000002	No Hit
CGGAAATGAACTCTTCAAATCTGATCAGCTGGTTAGCATGATGTAACAAG	7	0.17500000000000002	No Hit
CTTCATTTAAAAGATATTGATTAAAAAAATTCAACTGGTAAAAAAAATCT	7	0.17500000000000002	No Hit
CCACTCAGCTGATTTACAATTTATGCATTTAATGCATAGTTTAAAAAATC	7	0.17500000000000002	No Hit
CTTCTGGGGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGT	7	0.17500000000000002	No Hit
CGTGGTCTGTGGATTGTGTCTTGGGTGTTCTGAGCTTTGGGCTAATACCC	7	0.17500000000000002	No Hit
GCTACTGGGATTGGCAATGTTGAAAGCAAGTGGCGAGGCGGTGTTCAAAA	7	0.17500000000000002	No Hit
CTGGGGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAGA	7	0.17500000000000002	No Hit
GTGGGGGCTTGTTGTGAATCCACTTCACTACTTTGACGCCATTCTGAGCA	7	0.17500000000000002	No Hit
GCTGCTCAATGGGACTCAATCCACTTCCTGCTGAACGATCTCTGCTTATT	7	0.17500000000000002	No Hit
GGAGGCTGCTCAATGGGACTCAATCCACTTCCTGCTGAACGATCTCTGCT	7	0.17500000000000002	No Hit
TGGTTCTTTAGTAAATCAATGACTTCTCTGCTGAACTGAATCAGCTGAGG	7	0.17500000000000002	No Hit
CGGACCATGACTTTTGGCTCATTCGCTGCTCTGCTTCTTGACTAGGGAGG	7	0.17500000000000002	No Hit
GGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAGAATCA	7	0.17500000000000002	No Hit
CCCCTCGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTT	7	0.17500000000000002	No Hit
CCCTTATCAAACGGAGACAGACAGAGAAGGGAAGCATGAAATAACTTTCT	7	0.17500000000000002	No Hit
CCCTCGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTTC	7	0.17500000000000002	No Hit
CTGGGTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCAC	7	0.17500000000000002	No Hit
ATCCGCAAAGGCTAGCTTTTCCCAAATGCCAACATCAGCTACCATAATAA	7	0.17500000000000002	No Hit
GTCCCGGTTTCTGGTTTCAGCTTGCTGCAGCTGAGTCTCCAATTCAGCTT	7	0.17500000000000002	No Hit
CCACCTGTGGGATGGCTCCCAGTAGCTCCTCCACACTACTGTAGCCATAA	6	0.15	No Hit
CGCTGCCAATTTTGTATCTGTGGAGGCTGTTCACAGCACAGATTGCGTCA	6	0.15	No Hit
CATCCATTTGCCTAAGAATTTGATGTAATCATTGTTTTTAGAAGCTGAGT	6	0.15	No Hit
TGCCTGTGATACTTAGCACCTTGCCACCACAGTTATCAGAGAGGCGCCTG	6	0.15	No Hit
CCGGCACTGCTTTCCAAAATGGTGATGGTACAAAGGGATGAAATTACTGA	6	0.15	No Hit
TCTTGTCTCCCAAGTCTCATCAAGAGCAAACAGTGTAGGATGGGAGGATG	6	0.15	No Hit
ACCCAAACATGCTTCCAACAGTGAACAGTAACAGTAAAGCTAGACACTAT	6	0.15	No Hit
GTTGCTGAAGAGAATGAATATCAGGAAGGGGAACTGCTGGGAAAAATTTA	6	0.15	No Hit
CTTCAGGCATTTCACAAAGAGTGGCAAGCCTGCAGAACATTTCTAGAGCA	6	0.15	No Hit
AGATCACACACCCATTCACTGTACACAACTCTTCTTTGTTGCCCACAGAA	6	0.15	No Hit
CCGGTAGGTTATAAACATAGAGTAGAGTGTGGCAAGGAATCTTCAGTGGT	6	0.15	No Hit
CCCCACTCAGCTGATTTACAATTTATGCATTTAATGCATAGTTTAAAAAA	6	0.15	No Hit
TCCCCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGG	6	0.15	No Hit
CTCTCGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTTC	6	0.15	No Hit
GGCATTCGGAAATGGGGCTGGTGGCGCAGCAAGTCCACAACATCTTTGGA	6	0.15	No Hit
TCGCCTCTGGATAAAGAAATAGTGATTGGACTGGCCTCTTGGGCATGTCA	6	0.15	No Hit
ACCCAGAGCATTTTCTAACGACAAGTCTTCTACTTCTAGCGACAGGTTGT	6	0.15	No Hit
CGGCCATGCTTGGAGAAGGCTTCTTGGAGGAGCTGCTGTAGCTCCTTCCG	6	0.15	No Hit
GATAAACTCTGCTTCTCTGTGTCCTCTAAAAAACTTCTCACGGATTCTCT	6	0.15	No Hit
TTGTAGAGGAGCCATCATGTGACTGGGAAGAGCCCAGTGGGCTCTGGCGG	6	0.15	No Hit
GTCCTCGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTT	6	0.15	No Hit
GCTTCTGGGCCCGCTCTGCGCTGTCTTGGTTTATAAAGCGGAGAATAGCG	6	0.15	No Hit
CTGGCCATGAAATAAGAATCCATTTTCTGGCTTTCTAGAAAAAATAATAT	6	0.15	No Hit
GCCGGCAGGTTATAAACATAGAGTAGAGTGTGGCAAGGAATCTTCAGTGG	6	0.15	No Hit
GCTGGTAGTGATAGGTATGAAGCAAAGACCTCACATTCTTTGCAAACAAG	6	0.15	No Hit
CTCTATGAATGGATTTGCCCTTTTTGCCCAACAGATACAATCCATTGTAA	6	0.15	No Hit
CTGCTTTTTTGCTGGGCTTTCCACTGCATCCTTGCTAATCAGTAGCCCGG	6	0.15	No Hit
GTTCTCTTTACTGATGTCACACACACAGATAAACTCTGCTTCTCTGTGTC	6	0.15	No Hit
GCTGGTCTTCATGATTGACAGGGGAAAGGTCAAATGAACTTAGCCGGTTT	6	0.15	No Hit
GCCTGGGACTTGAGAAGTTTCAGTAAATCCTGAGTAAAGCGCTTCACCTG	6	0.15	No Hit
CGGCCAAAGTGGTGGTGGTAAGAGGGAATGAACTTGTTAAAGGGCATTCG	6	0.15	No Hit
CATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGTCACAG	5	0.125	No Hit
CTCCTGTGGAGAGTGACACCAGTATCTTCTTGCTGCCAATTTTGTATCTG	5	0.125	No Hit
TGGGGCAGGTCATAGTGCTGAGAACTGGGAATCACTGGCTTCATTTAAAA	5	0.125	No Hit
TGCCTGAGATCACTGAGTTCTAATGCAAAATTAACATCAGTTGACACAAG	5	0.125	No Hit
CTATGGACCATTAATAAAAGTGGCTTCTGGGGTCAAGACATGAAATAAGG	5	0.125	No Hit
GCTCAATGGGACTCAATCCACTTCCTGCTGAACGATCTCTGCTTATTTCT	5	0.125	No Hit
AGGCACACCAATAAGTCACCTAGAAGCCAGGAACTTTCCTGTCAACCGGA	5	0.125	No Hit
CGGCCGCAAAGACTTCCGGGTTATCAGTTGGATTTGTTTGCCAGACTCCA	5	0.125	No Hit
TCGTGATACTCCAACTCTTCAAAGAGAACTGGGCTGCAGTTTGTAGAGGA	5	0.125	No Hit
CGGCGGTGTTCAAAAGGTTTGGAGACATACTCCTCGGAGACCAAGACTGA	5	0.125	No Hit
GCGTCATGCAGGTTTCTCATCTGCACGATGGCTTTGAGCTGGTAATCAGT	5	0.125	No Hit
CCGGTTTTTCATGTCATTTTTTAACACTACAATTCTCTTATGACCATGTC	5	0.125	No Hit
CTCTTCAGTAGCTCAGTCAAAGTCATGAAACCATATTCACAGGGGTTGAG	5	0.125	No Hit
TCTCTGCTCTATGAATGGATTTGCCCTTTTTGCCCAACAGATACAATCCA	5	0.125	No Hit
CTACCCAAACATGCTTCCAACAGTGAACAGTAACAGTAAAGCTAGACACT	5	0.125	No Hit
CTGGCTTAAATGGATGACATCGAGACCATCTCTTTTCCTTAAACCTTACC	5	0.125	No Hit
CTGAGCTCAACACTCTTCACCTGGCCATGCTTGGAGAAGGCTTCTTGGAG	5	0.125	No Hit
GCTGGGTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCA	5	0.125	No Hit
TCCCAGATTTACAGCCAATTTTTAAATCACAAGAACAAGGATCAACCTTG	5	0.125	No Hit
GCGAGGCGGTGTTCAAAAGGTTTGGAGACATACTCCTCGGAGACCAAGAC	5	0.125	No Hit
CGGCACTGAAGCGGTTGGGCCACTGTTAGGCAGTGTGGCCAGGCTCCCAT	5	0.125	No Hit
CTCCTTTTCCATCCCTTTTGTTCCACAGCGATTACACAGAGGAACAGCTC	5	0.125	No Hit
GTACAAAGGGATGAAATTACTGATTGGTAGTATGCAAGATGGTTGGTTCT	5	0.125	No Hit
CTCAACCTTTAATTTTGTGACATTTTTATCAAAATGCTGGCCATGAAATA	5	0.125	No Hit
CCCTCTGCTTGTAGGTCATGGCCAGGTCTCTCCAATAGTTTGTTTCTTCC	5	0.125	No Hit
GCCAACATCAGCTACCATAATAATAAAAACCCAAACATGCTTCCAACAGT	5	0.125	No Hit
GCAAGGAATCTTCAGTGGTAACCTGGGGGGTAGATCGGAAATGAACTCTT	5	0.125	No Hit
CTGAGTTCGTTCTCTTTTTCGGATACAAATGACCATGTCATCATCTTGTT	5	0.125	No Hit
CCCGGATTCTCTGTACAACAGTTGTTGCTGACCGGCCAGAGGGGACTGAG	5	0.125	No Hit
TTTTCATAGATGTCTATAAATTTAAAGAGAGGCAAACAACAGGCAGGAGC	5	0.125	No Hit
GTCGCAGGCTCTGACGGAGTTTATCATCAGCAGCATTCTTTGCGGTAGCG	5	0.125	No Hit
GCCATCATGTGACTGGGAAGAGCCCAGTGGGCTCTGGCGGGCCTGGTTAC	5	0.125	No Hit
CTCTGGGAACTGAGGTTCTCTTTTTTTTGACTGCTTCTGTGAAGTGTCTC	5	0.125	No Hit
CCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGTCAC	5	0.125	No Hit
TGACCGGCCAGAGGGGACTGAGCAGTTTTCAATATCCCAGAAAACACCAA	5	0.125	No Hit
GGTAGTATGCAAGATGGTTGGTTCTTTAGTAAATCAATGACTTCTCTGCT	5	0.125	No Hit
TTGCCGGCAGGTTATAAACATAGAGTAGAGTGTGGCAAGGAATCTTCAGT	5	0.125	No Hit
GTGTGACCCACAGGTTTAAGGACATGTTTGGCTTCTTGAACAACCACAAA	5	0.125	No Hit
ACACCGTATTCCGTGACATCCCAGTCCTTTGAGAAACACCAATGATAAGC	5	0.125	No Hit
CTCCTGCGTGGGATATGTGTCTCTGTAATAGGTCTCTGTAATAAACCTCA	5	0.125	No Hit
AACCTCTCAATACACAATATGTCATTCAAATGCTGACTTAAATGGATGAC	5	0.125	No Hit
CCGGCAGGTTATAAACATAGAGTAGAGTGTGGCAAGGAATCTTCAGTGGT	5	0.125	No Hit
CCGGCCAAAGTGGTGGTGGTAAGAGGGAATGAACTTGTTAAAGGGCATTC	5	0.125	No Hit
CCAACAGTGAACAGTAACAGTAAAGCTAGACACTATTAAAGACGTCAAGA	5	0.125	No Hit
GGCTGCAGTTTGTAGAGGAGCCATCATGTGACTGGGAAGAGCCCAGTGGG	5	0.125	No Hit
TCTCAATACACAATATGTCATTCAAATGCTGACTTAAATGGATGACATCG	5	0.125	No Hit
GCTACCATAATAATAAAAACCCAAACATGCTTCCAACAGTGAACAGTAAC	5	0.125	No Hit
ATCATCTGAAAGTTGGAAACAAACATTTCCCTATGGACCATTAATAAAAG	5	0.125	No Hit
ACCATTACCTCTCAACACAATATGTCATTCAAATGCTGACTTAAATGGAT	5	0.125	No Hit
CTTGCGTAATTTCTAGGTCACCAAACTCCGCAGCATAGCAATCTGGAAAG	5	0.125	No Hit
ATCTGGGGCAGGTCATAGTGCTGAGAACTGGGAATCACTGGCTTCATTTA	5	0.125	No Hit
TGTAGAATCAGACAGTTTTCCCCCCATCCTAATTTAATTCTGTGTTCAAA	5	0.125	No Hit
GCCAATTTTGTATCTGTGGAGGCTGTTCACAGCACAGATTGCGTCATGCA	5	0.125	No Hit
CCGGGGGCTTCTGAGGAGGGTTTGGGAGGAGCAGAAGCAGATGCTGGGCT	5	0.125	No Hit
GGGGGGTAGATCGGAAATGAACTCTTCAAATCTGATCAGCTGGTTAGCAT	5	0.125	No Hit
GGCAGGTCATAGTGCTGAGAACTGGGAATCACTGGCTTCATTTAAAAGAT	5	0.125	No Hit
TGCTGGCCATGAAATAAGAATCCATTTTCTGGCTTTCTAGAAAAAATAAT	5	0.125	No Hit
CTCTGCTCTATGAATGGATTTGCCCTTTTTGCCCAACAGATACAATCCAT	5	0.125	No Hit
GTTGCCAAAGACATCTTCATTTTCCATTCGCTTCTGGGCCCGCTCTGCGC	5	0.125	No Hit
ACTGGCCTGGGACTTGAGAAGTTTCAGTAAATCCTGAGTAAACCGCTTCA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.025	0.0	0.0	0.0
8	0.0	0.025	0.0	0.0	0.0
9	0.0	0.025	0.0	0.0	0.0
10-11	0.0	0.025	0.0	0.0	0.0
12-13	0.0	0.025	0.0	0.0	0.0
14-15	0.0	0.075	0.0	0.0	0.0
16-17	0.0	0.125	0.0	0.0	0.0
18-19	0.0	0.125	0.0	0.0	0.0
20-21	0.0	0.125	0.0	0.0	0.0
22-23	0.0	0.125	0.0	0.0	0.0
24-25	0.0	0.125	0.0	0.0	0.0
26-27	0.0	0.125	0.0	0.0	0.0
28-29	0.0	0.125	0.0	0.0	0.0
30-31	0.0	0.125	0.0	0.0	0.0
32-33	0.0	0.125	0.0	0.0	0.0
34-35	0.0	0.1375	0.0	0.0	0.0
36-37	0.0	0.175	0.0	0.0	0.0
38-39	0.0	0.2	0.0	0.0	0.0
40-41	0.0	0.2	0.0	0.0	0.0
42-43	0.0	0.2	0.0	0.0	0.0
44-45	0.0	0.2	0.0	0.0	0.0
46-47	0.0	0.2	0.0	0.0	0.0
48-49	0.0	0.2	0.0	0.0	0.0
50-51	0.0	0.2	0.0	0.0	0.0
52-53	0.0	0.2	0.0	0.0	0.0
54-55	0.0	0.2	0.0	0.0	0.0
56-57	0.0	0.3	0.0	0.0	0.0
58-59	0.0	0.3	0.0	0.0	0.0
60-61	0.0	0.3	0.0	0.0	0.0
62-63	0.0	0.375	0.0	0.0	0.0
64-65	0.0	0.375	0.0	0.0	0.0
66-67	0.0	0.375	0.0	0.0	0.0
68-69	0.0	0.5125	0.0	0.0	0.0
70-71	0.0	0.8625	0.0	0.0	0.0
72-73	0.0	0.95	0.0	0.0	0.0
74-75	0.0	0.95	0.0	0.0	0.0
76-77	0.0	0.95	0.0	0.0	0.0
78-79	0.0	0.95	0.0	0.0	0.0
80-81	0.0	0.95	0.0	0.0	0.0
82-83	0.0	0.95	0.0	0.0	0.0
84-85	0.0	0.95	0.0	0.0	0.0
86-87	0.0	0.95	0.0	0.0	0.0
88-89	0.0	0.95	0.0	0.0	0.0
90-91	0.0	0.95	0.0	0.0	0.0
92-93	0.0	0.95	0.0	0.0	0.0
94-95	0.0	0.95	0.0	0.0	0.0
96-97	0.0	0.95	0.0	0.0	0.0
98-99	0.0	0.95	0.0	0.0	0.0
100-101	0.0	0.95	0.0	0.0	0.0
102-103	0.0	0.95	0.0	0.0	0.0
104-105	0.0	1.0	0.0	0.0	0.0
106-107	0.0	1.0	0.0	0.0	0.0
108-109	0.0	1.0	0.0	0.0	0.0
110-111	0.0	1.025	0.0	0.0	0.0
112-113	0.0	1.025	0.0	0.0	0.0
114-115	0.0	1.025	0.0	0.0	0.0
116-117	0.0	1.025	0.0	0.0	0.0
118-119	0.0	1.025	0.0	0.0	0.0
120-121	0.0	1.025	0.0	0.0	0.0
122-123	0.0	1.075	0.0	0.0	0.0
124-125	0.0	1.075	0.0	0.0	0.0
126-127	0.0	1.075	0.0	0.0	0.0
128-129	0.0	1.075	0.0	0.0	0.0
130-131	0.0	1.075	0.0	0.0	0.0
132-133	0.0	1.075	0.0	0.0	0.0
134-135	0.0	1.075	0.0	0.0	0.0
136-137	0.0	1.075	0.0	0.0	0.0
138-139	0.0	1.075	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTGAGA	15	0.009006455	131.38272	135-139
ATAGTCC	20	0.0036726785	31.95796	110-114
CATAGTC	20	0.0036726785	31.95796	110-114
AGTCCAT	20	0.0041281665	31.20821	105-109
AACCTAG	20	0.007029629	28.005262	90-94
AGTGAAA	20	0.007306203	27.785902	85-89
GTGAAAC	20	0.007306203	27.785902	85-89
ACTTTAG	20	0.007495312	27.64156	80-84
TCTATGC	25	0.009407844	26.605	7
CTATGCT	25	0.009407844	26.605	8
>>END_MODULE
Read 9196598 spots for SRR26281812.sra
Written 9196598 spots for SRR26281812.sra
Read 9196598 spots for SRR26281812.sra
Written 9196598 spots for SRR26281812.sra
Read 9196598 spots for SRR26281812.sra
Written 9196598 spots for SRR26281812.sra
Read 9196598 spots for SRR26281812.sra
Written 9196598 spots for SRR26281812.sra
Read 9196598 spots for SRR26281812.sra
Written 9196598 spots for SRR26281812.sra
Read 9196598 spots for SRR26281812.sra
Written 9196598 spots for SRR26281812.sra
Read 9196598 spots for SRR26281812.sra
Written 9196598 spots for SRR26281812.sra
Rejected 18012 READS because READLEN < 1
Read 9196603 spots for SRR26281812.sra
Written 9196603 spots for SRR26281812.sra
SRR ids: ['SRR26281812.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_va7hhh1v
SRR26281812.sra spots: 73572789
blocks: [[1, 9196598], [9196599, 18393196], [18393197, 27589794], [27589795, 36786392], [36786393, 45982990], [45982991, 55179588], [55179589, 64376186], [64376187, 73572789]]
SRR26281812 file size 23304592
SRR26281812 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR26281812 SRR26281812_1.fastq SRR26281812_2.fastq
Input file:	SRR26281812_1.fastq
Paired file:	SRR26281812_2.fastq
trimmed:	SRR26281812-trimmed-pair1.fastq, SRR26281812-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Mon Nov 10 20:05:09 2025 >> started

Mon Nov 10 20:11:22 2025 >> done (372.795s)
73557442 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
73557442 (100.00%) read pairs available; of these:
  185139 ( 0.25%) trimmed read pairs available after processing
73372303 (99.75%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 35	     917	  0.00%
 36	    1017	  0.00%
 37	    1260	  0.00%
 38	    1614	  0.00%
 39	    2020	  0.00%
 40	    2364	  0.00%
 41	    2793	  0.00%
 42	    2886	  0.00%
 43	    2995	  0.00%
 44	    2765	  0.00%
 45	    2783	  0.00%
 46	    3053	  0.00%
 47	    3784	  0.01%
 48	    4480	  0.01%
 49	    5191	  0.01%
 50	    6314	  0.01%
 51	    7517	  0.01%
 52	    7876	  0.01%
 53	    7973	  0.01%
 54	    7692	  0.01%
 55	    7778	  0.01%
 56	    8361	  0.01%
 57	    9169	  0.01%
 58	   10872	  0.01%
 59	   12286	  0.02%
 60	   13900	  0.02%
 61	   16011	  0.02%
 62	   17811	  0.02%
 63	   19491	  0.03%
 64	   20675	  0.03%
 65	   21715	  0.03%
 66	   21569	  0.03%
 67	   22309	  0.03%
 68	   23597	  0.03%
 69	   25594	  0.03%
 70	   28914	  0.04%
 71	   32346	  0.04%
 72	   37245	  0.05%
 73	   42445	  0.06%
 74	   47475	  0.06%
 75	   51594	  0.07%
 76	   54588	  0.07%
 77	   55266	  0.08%
 78	   57359	  0.08%
 79	   59320	  0.08%
 80	   63649	  0.09%
 81	   70631	  0.10%
 82	   80000	  0.11%
 83	   91993	  0.13%
 84	  103820	  0.14%
 85	  114451	  0.16%
 86	  121906	  0.17%
 87	  128103	  0.17%
 88	  130670	  0.18%
 89	  134054	  0.18%
 90	  138765	  0.19%
 91	  148145	  0.20%
 92	  161504	  0.22%
 93	  179818	  0.24%
 94	  199661	  0.27%
 95	  220157	  0.30%
 96	  237371	  0.32%
 97	  250353	  0.34%
 98	  257570	  0.35%
 99	  260669	  0.35%
100	  265417	  0.36%
101	  270558	  0.37%
102	  286531	  0.39%
103	  306721	  0.42%
104	  329367	  0.45%
105	  354739	  0.48%
106	  377580	  0.51%
107	  392571	  0.53%
108	  398924	  0.54%
109	  403961	  0.55%
110	  402034	  0.55%
111	  404291	  0.55%
112	  411301	  0.56%
113	  421026	  0.57%
114	  440920	  0.60%
115	  462634	  0.63%
116	  480119	  0.65%
117	  493511	  0.67%
118	  507205	  0.69%
119	  508064	  0.69%
120	  505386	  0.69%
121	  501536	  0.68%
122	  498571	  0.68%
123	  501779	  0.68%
124	  511290	  0.70%
125	  520935	  0.71%
126	  527835	  0.72%
127	  545246	  0.74%
128	  554338	  0.75%
129	  553910	  0.75%
130	  549671	  0.75%
131	  547454	  0.74%
132	  539663	  0.73%
133	  538372	  0.73%
134	  533278	  0.72%
135	  534603	  0.73%
136	  543846	  0.74%
137	  554644	  0.75%
138	  555439	  0.76%
139	  553116	  0.75%
140	  549106	  0.75%
141	  546318	  0.74%
142	  531383	  0.72%
143	  525487	  0.71%
144	  519776	  0.71%
145	  525889	  0.71%
146	  557704	  0.76%
147	  614980	  0.84%
148	 1174562	  1.60%
149	 5200925	  7.07%
150	21680211	 29.47%
151	18654441	 25.36%
73557442 reads passed initial QC


criterion=sequence-density
sequence-density=1.65
sequence-density-rank=1
fanout-score=2.16
fanout-score-rank=38
prefix-density=1.70
prefix-fanout=2.1
sequence=CTTTCAGGCTCTTGCTGGGAGCTCTAGGCACTGGGCTACAGGAAAGTGAGACTCAAGAGGAAGACAGAGAAGGTTGTAACGTAGAGAGAGTGAGTCATAAAGTTTCAAGCATGCCCCCCCCACCTCTCCCCACCCTTTGCCACTTGAAACTTACTAATCAAGAGAAACTTCCAAGCCAACGGAAGGAATGGTCGGATCTCACAGGCTGAGAATTTGTTCCCCTCCAAGCATTTCATGAAAAAGCTGCTTCTCATTAACCATGCGAACTCTCACAGTGATGTGAAGAGCTTGACAGATCTTTCAAAATAAAAAGTAATGACTTAGAAATGGCC


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=34
fanout-score=165.21
fanout-score-rank=1
prefix-density=2.27
prefix-fanout=1.2
sequence=TCCTGCAGCCCTTCAGACCGCCAGAACTCTTCTGCTGCCTGCCCAACTGCCTGCCTGCCTGTGCCGAGAGTTCCCAGCACCATGAGGGCCTGGATCTTCTTTCTCCTTTGCCTGGCCGGGAGGGCCCTGGCAGCCCCTCAGACGGAAGCTGCAGAAGAGATGGTGGCGGAGGAAACCGTGGTGGAGGAGACAGGGTTACCTGTGGGTGCCAACCCAGTCCAGGTGGAAATGGGAGAGTTTGAAGAAGGTGCAGAGGAAACTGTCGAGGAGGTGGTGGCTGAAAACCCCTGCCAGAACCATCACTGCAAACATGGCAAGGTGTGTGAGCTGGACGAGAGCAACACCCCCATGTGTGTGTGCCAGGACCCCACCAGCTGCCCAGCTCCCATTGGCGAGTTTGAAAAGGTGTGCAGCAATGACAACAAGACCTTCGACTCTTCCTGCCACTTCTTTGCGACCAAGTGCACCCTGGAGGGCACCAAGAAGGGCCACAAGCTCCACCTGGACTACA


criterion=sequence-density
sequence-density=1.85
sequence-density-rank=1
fanout-score=2.00
fanout-score-rank=32
prefix-density=1.79
prefix-fanout=2.0
sequence=CCTATAGCAGTTGAGTAAGAGAATCCCTAGTGAGGTATTGGTCTGCCCATGCCTCCCCTTGGCTCACCAAAATTAAAACCAACAGCCAAACATGGAAAGAAACTGCCTGAAGAGTGGAGGAGACAGCAAGGTCCCGGGCTGCTGGGAGTCTGTTTTGTGCATCAGAGCTCTGGATTATTCTGTCAGTCGTTGACCTAGGCTACCAAGACTTGCCATCTGGTTCTGACTGGCCTCACAGCGTCCTTGCCCAAGTTTTGTATCTGTGTCTGTAACTTCCCTCTGCCTACCTCCAAACTACCAGGGGAAGACCCCAGGAAGCCCACATCCCCCCCCCAA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=34
fanout-score=71.44
fanout-score-rank=1
prefix-density=0.48
prefix-fanout=2.0
sequence=TGGGGGCAGGGTGGCTCCATCCAAGGAGTCTCAGCAACAGGAACCCCACAAGGCAACAGTCTACTAATGATTACTATAGAGTTGGGAGACAGGGCTGCTCCACAGACACATGAATAGGTCAAATCTTTTATAAATAAACATCCAGTGAAGAGAAAAATGACAACTCTAATTCATTCTTATACACAAGGCGCACTCTAGGGCGATGGGTGGGGTGACCAGAGGCTGAAATCGTCACTGGTCAGGGGCCTGGGTGGGAGATGAGTGGGCCCAGATGTCCACAGCACGGGGCAGTAGGGGCAAGGCGGGGCAACAGACAATGGGCACTAGGACACTGCACATTTACAAGACCAACTACTGAGGGGGCAGCTGCCCGTGCATGTGTCAGGCTGTTCCTTCTGGAATGAGGAGGGGTGATCTTTACATCCTATCCTGTGACTGGTGGTGGAAAAAGACTACTTTGTGGAGAGGATTAGGGCCACGATGAGACCGTAGAGGCCAAGCACCTCCGCAA
SRR26281812 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Nov 10 20:12:36
                             Started mapping on |	Nov 10 20:12:36
                                    Finished on |	Nov 10 20:23:22
       Mapping speed, Million of reads per hour |	409.92

                          Number of input reads |	73557442
                      Average input read length |	278
                                    UNIQUE READS:
                   Uniquely mapped reads number |	68020646
                        Uniquely mapped reads % |	92.47%
                          Average mapped length |	277.14
                       Number of splices: Total |	76072710
            Number of splices: Annotated (sjdb) |	73907041
                       Number of splices: GT/AG |	74943732
                       Number of splices: GC/AG |	474975
                       Number of splices: AT/AC |	54015
               Number of splices: Non-canonical |	599988
                      Mismatch rate per base, % |	0.43%
                         Deletion rate per base |	0.03%
                        Deletion average length |	1.70
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.68
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	5523511
             % of reads mapped to multiple loci |	7.51%
        Number of reads mapped to too many loci |	7283
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.01%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	13319	13319	13319
N_multimapping	5523511	5523511	5523511
N_noFeature	7383647	8354854	66471155
N_ambiguous	1099828	515686	10279
UnstrandedReadsAssigned:59537171 PositiveStrandReadsAssigned:59150106 NegativeStrandReadsAssigned:1539212
Dataset is classified positive stranded
MeadianReadLen=150 20thPercentileLength=126 echo kmer=121
SRR26281812 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 31,715
[index] number of k-mers: 49,915,185
[index] number of equivalence classes: 70,351
[quant] running in paired-end mode
[quant] will process pair 1: SRR26281812-trimmed-pair1.fastq
                             SRR26281812-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 73,557,442 reads, 63,052,237 reads pseudoaligned
[quant] estimated average fragment length: 186.46
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,062 rounds

  31716 SRR26281812.ke.tsv
  32883 SRR26281812.se.tsv
  64599 total
==> SRR26281812.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	769.54	106284	3214.57
ENSRNOT00000040993.4	1039	853.54	181221	4941.64
ENSRNOT00000050156.3	1545	1359.54	1.03047e+06	17641.3
ENSRNOT00000043693.3	684	498.731	215199	10042.9
ENSRNOT00000046201.3	204	60.0645	52706	20423.4
ENSRNOT00000046108.3	681	495.731	283934	13330.8
ENSRNOT00000049683.3	784	598.584	295909	11505.9
ENSRNOT00000041241.3	348	173.771	23875	3197.81
ENSRNOT00000044582.3	297	129.75	20089.3	3603.65

==> SRR26281812.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	10
ENSRNOG00000058808	0
ENSRNOG00000061316	1127
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	10
SRR26281812 completed mapping pipeline successfully
