Starting /dee2/code/volunteer_pipeline.sh SRR26281815
    current disk space = 687118740
    free memory = 197337968 
SRR26281815 SRAfilesize
d6f43372d17bdd0c2e3637f008139401  SRR26281815.sra
SRR26281815.sra file validated
SRR26281815 is paired end
SRR26281815 is conventional basespace
SRR26281815 read1 length is 42-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR26281815_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	42-151
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.289	37.0	37.0	37.0	37.0	37.0
2	36.19	37.0	37.0	37.0	37.0	37.0
3	36.294	37.0	37.0	37.0	37.0	37.0
4	36.3445	37.0	37.0	37.0	37.0	37.0
5	36.4325	37.0	37.0	37.0	37.0	37.0
6	36.4275	37.0	37.0	37.0	37.0	37.0
7	36.277	37.0	37.0	37.0	37.0	37.0
8	36.4725	37.0	37.0	37.0	37.0	37.0
9	36.4295	37.0	37.0	37.0	37.0	37.0
10-14	36.375299999999996	37.0	37.0	37.0	37.0	37.0
15-19	36.355900000000005	37.0	37.0	37.0	37.0	37.0
20-24	36.3708	37.0	37.0	37.0	37.0	37.0
25-29	36.29625	37.0	37.0	37.0	37.0	37.0
30-34	36.2732	37.0	37.0	37.0	37.0	37.0
35-39	36.2655	37.0	37.0	37.0	37.0	37.0
40-44	36.17821615403851	37.0	37.0	37.0	37.0	37.0
45-49	36.14168542135534	37.0	37.0	37.0	37.0	37.0
50-54	36.1415842271899	37.0	37.0	37.0	37.0	37.0
55-59	36.093785607766	37.0	37.0	37.0	37.0	37.0
60-64	36.127686852246164	37.0	37.0	37.0	37.0	37.0
65-69	36.16318140611384	37.0	37.0	37.0	37.0	37.0
70-74	36.10015853242307	37.0	37.0	37.0	37.0	37.0
75-79	36.03038987268242	37.0	37.0	37.0	37.0	37.0
80-84	36.015208000416855	37.0	37.0	37.0	37.0	37.0
85-89	36.107986220037596	37.0	37.0	37.0	37.0	37.0
90-94	35.940046028681934	37.0	37.0	37.0	37.0	37.0
95-99	35.9870712980306	37.0	37.0	37.0	37.0	37.0
100-104	35.916784717828975	37.0	37.0	37.0	37.0	37.0
105-109	35.853297119503985	37.0	37.0	37.0	37.0	37.0
110-114	35.84690789341643	37.0	37.0	37.0	37.0	37.0
115-119	35.79552546129294	37.0	37.0	37.0	37.0	37.0
120-124	35.81351054059968	37.0	37.0	37.0	37.0	37.0
125-129	35.69022027541325	37.0	37.0	37.0	37.0	37.0
130-134	35.77641723118431	37.0	37.0	37.0	37.0	37.0
135-139	35.68460642584055	37.0	37.0	37.0	37.0	37.0
140-144	35.56958546253085	37.0	37.0	37.0	37.0	37.0
145-149	35.55567861478941	37.0	37.0	37.0	37.0	37.0
150-151	35.94741006130823	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	0.0
26	4.0
27	11.0
28	18.0
29	22.0
30	49.0
31	57.0
32	78.0
33	127.0
34	201.0
35	351.0
36	2568.0
37	513.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.175000000000004	26.474999999999998	15.425	26.924999999999997
2	31.922884326489736	22.55883825738608	24.912368552829246	20.605908863294943
3	24.025	25.4	28.299999999999997	22.275
4	20.1	33.2	26.125	20.575
5	21.525	39.35	22.45	16.675
6	19.6	36.199999999999996	23.125	21.075
7	18.8	23.5	36.65	21.05
8	22.95	25.124999999999996	26.625	25.3
9	22.2	22.35	29.275000000000002	26.174999999999997
10-14	23.395	28.415000000000003	26.369999999999997	21.82
15-19	22.264999999999997	26.815	27.450000000000003	23.47
20-24	22.845	28.935	25.705	22.515
25-29	22.191109555477773	28.25141257062853	26.09630481524076	23.461173058652932
30-34	23.255	28.985	26.345000000000002	21.415
35-39	22.64	27.47	26.97	22.919999999999998
40-44	23.092309230923092	27.312731273127312	27.987798779877988	21.607160716071608
45-49	21.500375093773442	28.067016754188543	27.12678169542386	23.305826456614152
50-54	21.53291975185111	28.16690014008405	27.16629977986792	23.133880328196916
55-59	22.48748748748749	27.17217217217217	28.78878878878879	21.55155155155155
60-64	21.51803607214429	27.79559118236473	27.895791583166336	22.79058116232465
65-69	22.277426477968483	27.80788919000301	27.05008531566797	22.864599016360536
70-74	23.583815028901732	28.35385775320432	25.73008293541091	22.332244282483035
75-79	22.417006699914364	27.761825600725405	26.235454133293036	23.585713566067202
80-84	23.22629582806574	27.585335018963335	28.581542351453855	20.606826801517066
85-89	21.914654113794516	26.698731068358576	28.48444535407286	22.902169463774047
90-94	22.44212484497726	26.59673418768086	28.64303431169905	22.31810665564283
95-99	22.927974947807932	29.58768267223382	25.855949895615865	21.628392484342378
100-104	22.28822998992203	29.27915981541399	26.10725083541081	22.32535935925317
105-109	23.10063267182177	27.53474287568269	27.10214675823284	22.262477694262696
110-114	21.90359294800268	28.503682213791564	25.864762329837088	23.72796250836867
115-119	22.595375722543352	28.641618497109828	25.734104046242773	23.028901734104046
120-124	22.77801242985579	29.57822965063658	25.5536112954806	22.09014662402703
125-129	21.998219282625286	28.230730094123636	26.69168150597812	23.079369117272957
130-134	22.53020134228188	28.248322147651006	26.738255033557046	22.483221476510067
135-139	22.63737816075717	28.125441446531994	24.473795733860715	24.76338465885012
140-144	22.10542237011268	29.19912274067912	26.272404144294033	22.423050744914164
145-149	22.951349604008406	28.083077420397608	25.375787942459997	23.589785033133992
150-151	22.096399535423924	12.340301974448316	35.743321718931476	29.819976771196284
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	4.0
26	10.5
27	10.0
28	13.0
29	18.5
30	18.0
31	22.0
32	25.5
33	44.0
34	61.5
35	83.0
36	123.0
37	129.5
38	130.5
39	144.0
40	157.5
41	175.0
42	170.5
43	184.0
44	238.0
45	243.5
46	221.5
47	190.0
48	180.5
49	219.0
50	214.0
51	190.0
52	167.5
53	134.5
54	100.5
55	74.0
56	61.0
57	59.0
58	68.0
59	55.0
60	30.0
61	23.5
62	15.5
63	6.5
64	6.5
65	6.0
66	2.5
67	0.5
68	0.0
69	0.5
70	1.0
71	0.5
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.15
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.005
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
40-44	1.0
45-49	1.0
50-54	1.0
55-59	3.0
60-64	6.0
65-69	6.0
70-74	7.0
75-79	12.0
80-84	40.0
85-89	39.0
90-94	28.0
95-99	60.0
100-104	63.0
105-109	99.0
110-114	114.0
115-119	150.0
120-124	154.0
125-129	170.0
130-134	159.0
135-139	167.0
140-144	161.0
145-149	485.0
150-152	2074.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	39.70276008492569	14.025000000000002
2	20.240622788393487	14.299999999999999
3	14.64968152866242	15.525
4	7.43099787685775	10.5
5	5.095541401273886	9.0
6	4.033970276008493	8.55
7	3.5385704175513095	8.75
8	1.48619957537155	4.2
9	1.2738853503184715	4.05
>10	2.547770700636943	11.1
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCTTCTG	22	0.5499999999999999	No Hit
GGATATTCCAAGCTGATTGAGCTACTAGAAGCCGTGCCTCATGTGTTGCA	22	0.5499999999999999	No Hit
AGAAATTCCAATACCAGTAAAGAAGGGGAACCTGTCCTGTAACTCCTCAC	19	0.475	No Hit
TGGTCATAAGAGAATTGTAGTGTTAAAAAATGACATGAAAAACCGGCTAA	17	0.42500000000000004	No Hit
CCTCCCAACACTGACCCTTGGCTTCTTCGTTCTAAAAGCCCTGTGAGAAA	15	0.375	No Hit
CATCACCATTTTGGAAAGCAGTGCCGGGTTTCAGACTATGGATATTCCAA	14	0.35000000000000003	No Hit
ACTAAATATGTAGGAGAAACACTGCAGCCCAAAACTTATGGCTACAGTAG	14	0.35000000000000003	No Hit
CTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCTTCTGCTCCTCC	14	0.35000000000000003	No Hit
GTTCATTTGACCTTTCCCCTGTCAATCATGAAGACCAGCCTGCAGTCAGC	13	0.325	No Hit
AGTAAAGAAGGGGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCAT	13	0.325	No Hit
CCAATGTGGCCTCATCCCATTTTTTAAAAAAGTTTCCATTTGGGGATTCC	13	0.325	No Hit
CAGAAGCTCTGCCATGTTGTAAAGGTTGCTGATATGGAGTCTGGCAAACA	12	0.3	No Hit
AGACTATTGTGAATTGTGGTGGTTGGGAAGAAATAAGCAGAGATCGTTCA	12	0.3	No Hit
GTTTAAGGAAAAGAGATGGTCTCGATGTCATCCATTTAAGTCAGCATTTG	12	0.3	No Hit
GGTCATTTGTATCCGAAAAAGAGAACGAACTCAGGATGAAATAGAAAGGA	12	0.3	No Hit
ACTGAAACTTCTCAAGTCCCAGGCCAGCAAGCAGGTCATTGTGAGCGATT	12	0.3	No Hit
CAGTTCTCTTTGAAGAGTTGGAGTATCACGAATCTGTGTGCAAGCAGCAC	12	0.3	No Hit
CTGAAGCCTTGTTACATCATGCTAACCAGCTGATCAGATTTGAAGAGTTC	11	0.27499999999999997	No Hit
AGCAGCACTGCTCGAACAAGGACTTCAGCGAACTTGTATTTGATCCAGAC	11	0.27499999999999997	No Hit
CATTTCGTCTCCAAGACTATGATGTCAGTTCAGTCTCAGCTTTGACACAG	11	0.27499999999999997	No Hit
CTTCGCCGCCCGAGCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCC	11	0.27499999999999997	No Hit
GTGGAAAGCCCAGCAAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGC	11	0.27499999999999997	No Hit
CAGGAAAGTTCCTGGCTTCTAGGTGACTTATTGGTGTGCCTTGTTTTTTA	11	0.27499999999999997	No Hit
GGAAAAGAGATGGTCTCGATGTCATCCATTTAAGTCAGCATTTGAATGAC	10	0.25	No Hit
GTCAAATTGGCAGCCAACTTTTCTTTTGCACCTGTGACCAAGCTCTAACT	10	0.25	No Hit
CTCATGTGTTGCAGATTCTTGGAATGGGCTCCAAGCGTCTACTGACCCTC	10	0.25	No Hit
CGATGCTAAGCCGTGGCTTTGGAAATTCTCTGGTTGCTTTTCTCTTCCTG	10	0.25	No Hit
GTTTCAGACTATGGATATTCCAAGCTGATTGAGCTACTAGAAGCCGTGCC	10	0.25	No Hit
GTGAACATTGCCACTGCTCAGAATGGCGTCAAAGTAGTGAAGTGGATTCA	10	0.25	No Hit
CACGAATCTGTGTGCAAGCAGCACTGCTCGAACAAGGACTTCAGCGAACT	10	0.25	No Hit
TCTGCTTCTGCTCCTCCCAAACCCTCCTCAGAAGCCCCCGGGCTACTGAT	10	0.25	No Hit
AGAATATGCTAAAACTTTTGGTTATACATTTCGTCTCCAAGACTATGATG	10	0.25	No Hit
AGAAACCAGAGTTTTATCTGTAATTTGAAGGCCAAGGTTGATCCTTGTTC	10	0.25	No Hit
TGTCTATTCTTCAGGATGCTCCTGCCTGTTGTTTGCCTCTCTTTAAATTT	10	0.25	No Hit
CGTCAAAGTAGTGAAGTGGATTCACAACAAGCCCCCACCTCCCAACACTG	10	0.25	No Hit
TGATTTTTTAAACTATGCATTAAATGCATAAATTGTAAATCAGCTGAGTG	10	0.25	No Hit
CAACAAGCCCCCACCTCCCAACACTGACCCTTGGCTTCTTCGTTCTAAAA	9	0.22499999999999998	No Hit
GGGAGACACTTCACAGAAGCAGTCAAAAAAAAGAGAACCTCAGTTCCCAG	9	0.22499999999999998	No Hit
CACGAGGGCACTGTGCCTTTGCTGAGCTTTCCAGATTGCTATGCTGCGGA	9	0.22499999999999998	No Hit
GGCCTTCTCTTCTGGGTGCCTGAGGTATCCACTTATGAGTGGCCTCTCAC	9	0.22499999999999998	No Hit
ATAATATCAGAGATCCCAGACACAACTATTTGCTTGTCCCAACAAGATGA	9	0.22499999999999998	No Hit
TGGATTCACAACAAGCCCCCACCTCCCAACACTGACCCTTGGCTTCTTCG	9	0.22499999999999998	No Hit
GTGGATTCACAACAAGCCCCCACCTCCCAACACTGACCCTTGGCTTCTTC	9	0.22499999999999998	No Hit
ACAAAAAACACAAACTTTAAAGGTTTACAGGAGCTGTGCCGAATGGAACC	9	0.22499999999999998	No Hit
GCGCTTTACTCAGGATTTACTGAAACTTCTCAAGTCCCAGGCCAGCAAGC	9	0.22499999999999998	No Hit
CCAGTCTAGGCAAAGCTGCTTCTAAATTTGGGTCACCAGAAGTTGCAGTC	9	0.22499999999999998	No Hit
GGAAAGTTCCTGGCTTCTAGGTGACTTATTGGTGTGCCTTGTTTTTTATG	9	0.22499999999999998	No Hit
AGCTGATCAGATTTGAAGAGTTCATTTCCGATCTACCCCCCAGGTTACCA	9	0.22499999999999998	No Hit
AACAAAAGGGATGGAAAAGGAGACCTCGTTGTTGGCAACCAGTCTAGGCA	9	0.22499999999999998	No Hit
CTGAGCTACTGAAGAGTCTGCCTTCTTTAGTAGAGGTTTTTATGAATGAT	9	0.22499999999999998	No Hit
GCCTTCTTTAGTAGAGGTTTTTATGAATGATAAGGCAGAAGAGTGTGTGA	9	0.22499999999999998	No Hit
ACTATTTGCTTGTCCCAACAAGATGATGACATGGTCATTTGTATCCGAAA	9	0.22499999999999998	No Hit
GGAGGAGCTACTGGGAGCCATCCCACAGGTGGTCTGGATAAAAGGACATG	9	0.22499999999999998	No Hit
CAAGATGATGACATGGTCATTTGTATCCGAAAAAGAGAACGAACTCAGGA	9	0.22499999999999998	No Hit
GTCTATTCTTCAGGATGCTCCTGCCTGTTGTTTGCCTCTCTTTAAATTTA	8	0.2	No Hit
CCCATCTGCATTCAGCCAGCTGATCGCCTCAAGGCAAGTCAGTCCTCTGT	8	0.2	No Hit
ATTTGCTTGTCCCAACAAGATGATGACATGGTCATTTGTATCCGAAAAAG	8	0.2	No Hit
AAACAATGTCTATTCTTCAGGATGCTCCTGCCTGTTGTTTGCCTCTCTTT	8	0.2	No Hit
AGAAGGGGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCT	8	0.2	No Hit
AAGATGATGACATGGTCATTTGTATCCGAAAAAGAGAACGAACTCAGGAT	8	0.2	No Hit
CAGTAAAGAAGGGGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCA	8	0.2	No Hit
TGTGAATTGTGGTGGTTGGGAAGAAATAAGCAGAGATCGTTCAGCAGGAA	8	0.2	No Hit
CGGGTTTCAGACTATGGATATTCCAAGCTGATTGAGCTACTAGAAGCCGT	8	0.2	No Hit
CAACTCTCTCCACCTAAATGTGGCACCTCCGGTTTGTTTAAAAGGCTCAC	8	0.2	No Hit
GGTTGATCCTTGTTCTTGTGATTTAAAAATTGGCTGTAAATCTGGGAAGC	8	0.2	No Hit
GCTAAAACTTTTGGTTATACATTTCGTCTCCAAGACTATGATGTCAGTTC	8	0.2	No Hit
CGAGGGCACTGTGCCTTTGCTGAGCTTTCCAGATTGCTATGCTGCGGAGT	8	0.2	No Hit
GCCACTTTTATTAATGGTCCATAGGGAAATGTTTGTTTCCAACTTTCAGA	8	0.2	No Hit
CGGCTAAGTTCATTTGACCTTTCCCCTGTCAATCATGAAGACCAGCCTGC	8	0.2	No Hit
AAATAAGCTACCCATCTGCATTCAGCCAGCTGATCGCCTCAAGGCAAGTC	8	0.2	No Hit
AGTGAACATTGCCACTGCTCAGAATGGCGTCAAAGTAGTGAAGTGGATTC	8	0.2	No Hit
GGTTTCATGACTTTGACTGAGCTACTGAAGAGTCTGCCTTCTTTAGTAGA	8	0.2	No Hit
GTGTTTGTGACTTGATTGATATAATATCAGAGATCCCAGACACAACTATT	8	0.2	No Hit
GGGATGGAAAAGGAGACCTCGTTGTTGGCAACCAGTCTAGGCAAAGCTGC	8	0.2	No Hit
CAGACACAACTATTTGCTTGTCCCAACAAGATGATGACATGGTCATTTGT	8	0.2	No Hit
GCTGATGATAAACTCCGTCAGAGCCTGCGAAGGTTTGCAAATACGCACAC	7	0.17500000000000002	No Hit
TCCAATACCAGTAAAGAAGGGGAACCTGTCCTGTAACTCCTCACCCAGTA	7	0.17500000000000002	No Hit
AAGCGGAGAAGGAGCTGGTGGCTGAGGGTGGAAGAATGAAAGAAAAGAAA	7	0.17500000000000002	No Hit
GCTGATCAGATTTGAAGAGTTCATTTCCGATCTACCCCCCAGGTTACCAC	7	0.17500000000000002	No Hit
TGCAAATGGTGTTGACATCCAGGTCAGCAATGTGGACTACAGGCTGTCCC	7	0.17500000000000002	No Hit
CCTCACTTGTGCACCAACTCTCTCCACCTAAATGTGGCACCTCCGGTTTG	7	0.17500000000000002	No Hit
CCTCTACAAACTGCAGCCCAGTTCTCTTTGAAGAGTTGGAGTATCACGAA	7	0.17500000000000002	No Hit
GCTACCCATCTGCATTCAGCCAGCTGATCGCCTCAAGGCAAGTCAGTCCT	7	0.17500000000000002	No Hit
GAAATAAGCAGAGATCGTTCAGCAGGAAGTGGATTGAGTCCCATTGAGCA	7	0.17500000000000002	No Hit
GCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCCCATTTTACCATGG	7	0.17500000000000002	No Hit
AGGGGACTGTCTTCCAAATAAGCTACCCATCTGCATTCAGCCAGCTGATC	7	0.17500000000000002	No Hit
GAACATTGCCACTGCTCAGAATGGCGTCAAAGTAGTGAAGTGGATTCACA	7	0.17500000000000002	No Hit
GCCTGGCCACACTGCCTAACAGTGGCCCAACCGCTTCAGTGCCCACACTG	7	0.17500000000000002	No Hit
GTGGATTGAGTCCCATTGAGCAGCCTCCATCATTTGTTTAAGCAAATGTA	7	0.17500000000000002	No Hit
AAATCCTTCCCAGTGGGGACCTCACAGAGATTGGTGAGAAGGGTGTGAAC	7	0.17500000000000002	No Hit
TCTCCGAGGAGTATGTCTCCAAACCTTTTGAACACCGCCTCGCCACTTGC	7	0.17500000000000002	No Hit
ATATGGTTTCATGACTTTGACTGAGCTACTGAAGAGTCTGCCTTCTTTAG	7	0.17500000000000002	No Hit
GATTGAGTGGCAAGAGAGCCATTTTCCGGTTGACAGGAAAGTTCCTGGCT	7	0.17500000000000002	No Hit
CTTCAGTGCCCACACTGAAAAACACAGGTGTGAGGGAGACACTTCACAGA	7	0.17500000000000002	No Hit
GAGAAACACTGCAGCCCAAAACTTATGGCTACAGTAGTGTGGAGGAGCTA	7	0.17500000000000002	No Hit
GGGTAAGGTTTAAGGAAAAGAGATGGTCTCGATGTCATCCATTTAAGTCA	7	0.17500000000000002	No Hit
CAGTCACCCAGTGCCAAAGCCATGCCTGGGAAAGGGTTGCAGGCAAATTC	7	0.17500000000000002	No Hit
AAAAATGACATGAAAAACCGGCTAAGTTCATTTGACCTTTCCCCTGTCAA	7	0.17500000000000002	No Hit
AGAAAGGCCTGCTAGCCCGCGCCTCCCTGGGATCTTCGCCGCCCGAGCTG	7	0.17500000000000002	No Hit
ATGCAGTGGAAAGCCCAGCAAAAAAGCAGCCCAAAAATAGAGTCAAATTG	7	0.17500000000000002	No Hit
GCCAGGTGAAGAGTGTTGAGCTCAGTCCCCATACTGATTACCAGCTCAAA	7	0.17500000000000002	No Hit
AGCTGAATAATTGTCAGGTAACTGTTGCTCACATAAACGCTACCGCAAAG	7	0.17500000000000002	No Hit
CCCCGGGCTACTGATTAGCAAGGATGCAGTGGAAAGCCCAGCAAAAAAGC	7	0.17500000000000002	No Hit
CTGGGAAAGGGTTGCAGGCAAATTCTGGATCTGCTACAAAAAACACAAAC	7	0.17500000000000002	No Hit
GAAAAAACAGAAATTCCAATACCAGTAAAGAAGGGGAACCTGTCCTGTAA	7	0.17500000000000002	No Hit
AGTGGATTGAGTCCCATTGAGCAGCCTCCATCATTTGTTTAAGCAAATGT	7	0.17500000000000002	No Hit
CTTTAATAGTGTCTAGCTTTACTGTTACTGTTCACTGTTGGAAGCATGTT	7	0.17500000000000002	No Hit
AACAAGATGATGACATGGTCATTTGTATCCGAAAAAGAGAACGAACTCAG	7	0.17500000000000002	No Hit
CCCACAGGTGGTCTGGATAAAAGGACATGGTCATAAGAGAATTGTAGTGT	7	0.17500000000000002	No Hit
GTGCTTCAAATGGTGTGGGTTCAATATTATTTCCACTAAGAAGAAACCAG	7	0.17500000000000002	No Hit
CTCCGTTTGATAAGGGTAAGGTTTAAGGAAAAGAGATGGTCTCGATGTCA	7	0.17500000000000002	No Hit
CGGCAAGAAAGGCCTGCTAGCCCGCGCCTCCCTGGGATCTTCGCCGCCCG	7	0.17500000000000002	No Hit
GTTTTATCTGTAATTTGAAGGCCAAGGTTGATCCTTGTTCTTGTGATTTA	7	0.17500000000000002	No Hit
AGGAGCTACTGGGAGCCATCCCACAGGTGGTCTGGATAAAAGGACATGGT	7	0.17500000000000002	No Hit
ATGAAAAGAAGTATGGACACAAGTTAAATGTGTCTGATTTATATAAATTA	7	0.17500000000000002	No Hit
GGGGAAAACTGTCTGATTCTACACACAAAAATAGGCTTAAAAACCTTATT	7	0.17500000000000002	No Hit
TGCAGATTCTTGGAATGGGCTCCAAGCGTCTACTGACCCTCACTCATAGG	7	0.17500000000000002	No Hit
GGGAAATGTTTGTTTCCAACTTTCAGATGAACTTTTTCTTACTTTATTTC	7	0.17500000000000002	No Hit
CAAAACTTATGGCTACAGTAGTGTGGAGGAGCTACTGGGAGCCATCCCAC	7	0.17500000000000002	No Hit
CGCACACTGCTCCAGCTACTGTGGTTCTTGTGTCAACTGATGTTAATTTT	7	0.17500000000000002	No Hit
TGTGATTTAAAAATTGGCTGTAAATCTGGGAAGCTCTCAATGTGAAGATG	7	0.17500000000000002	No Hit
GGGCACTGTGCCTTTGCTGAGCTTTCCAGATTGCTATGCTGCGGAGTTTG	7	0.17500000000000002	No Hit
GGCTACAGTAGTGTGGAGGAGCTACTGGGAGCCATCCCACAGGTGGTCTG	7	0.17500000000000002	No Hit
CCTTAAACCTGTGGGTCACACACTGGGGCATGATTGAGTGGCAAGAGAGC	7	0.17500000000000002	No Hit
TGAAGTGCTTCAAATGGTGTGGGTTCAATATTATTTCCACTAAGAAGAAA	7	0.17500000000000002	No Hit
AGCTACCCATCTGCATTCAGCCAGCTGATCGCCTCAAGGCAAGTCAGTCC	6	0.15	No Hit
ATCGGAATGTAAGCGGAGAAGGAGCTGGTGGCTGAGGGTGGAAGAATGAA	6	0.15	No Hit
AATGGCGTCAAAGTAGTGAAGTGGATTCACAACAAGCCCCCACCTCCCAA	6	0.15	No Hit
TGTCAGTTCAGTCTCAGCTTTGACACAGAAGCTCTGCCATGTTGTAAAGG	6	0.15	No Hit
GTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCTTCTGCTCCTCCCA	6	0.15	No Hit
AGCTGCTTCTAAATTTGGGTCACCAGAAGTTGCAGTCACTGGACAGGTGC	6	0.15	No Hit
CCCCAGGTTACCACTGAAGATTCCTTGCCACACTCTACTCTATGTTTATA	6	0.15	No Hit
TGAACAGTCACCCAGTGCCAAAGCCATGCCTGGGAAAGGGTTGCAGGCAA	6	0.15	No Hit
GGAAGGAGCTACAGCAGCTCCTCCAAGAAGCCTTCTCCAAGCATGGCCAG	6	0.15	No Hit
AAGAAGGGGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGC	6	0.15	No Hit
AGAAATAAGCAGAGATCGTTCAGCAGGAAGTGGATTGAGTCCCATTGAGC	6	0.15	No Hit
GCAGGTCATTGTGAGCGATTTCTCACAGGCTTATCATTGGTGTTTCTCAA	6	0.15	No Hit
GGCCAGCAAGCAGGTCATTGTGAGCGATTTCTCACAGGCTTATCATTGGT	6	0.15	No Hit
AGGAGAAACACTGCAGCCCAAAACTTATGGCTACAGTAGTGTGGAGGAGC	6	0.15	No Hit
GCTGCTTCTAAATTTGGGTCACCAGAAGTTGCAGTCACTGGACAGGTGCT	6	0.15	No Hit
AGAAGCTCTGCCATGTTGTAAAGGTTGCTGATATGGAGTCTGGCAAACAA	6	0.15	No Hit
CCTCCCTGGGATCTTCGCCGCCCGAGCTGGACATCATGCCACAGGAAGAG	6	0.15	No Hit
GTTTACAGGAGCTGTGCCGAATGGAACCAAAGTCTGGCAATAGAATCCGT	6	0.15	No Hit
CGCCGCCCGAGCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCCCAT	6	0.15	No Hit
GCAAGGATGCAGTGGAAAGCCCAGCAAAAAAGCAGCCCAAAAATAGAGTC	6	0.15	No Hit
AACTCTCTCCACCTAAATGTGGCACCTCCGGTTTGTTTAAAAGGCTCACT	6	0.15	No Hit
GGGGACTGTCTTCCAAATAAGCTACCCATCTGCATTCAGCCAGCTGATCG	6	0.15	No Hit
GAAACAATGTCTATTCTTCAGGATGCTCCTGCCTGTTGTTTGCCTCTCTT	6	0.15	No Hit
CTTTTGTGGTTCTCTCTTTGAAAGTGTTCGCACCTCAAGTTCATAGTCTC	6	0.15	No Hit
TTGCCACTGCTCAGAATGGCGTCAAAGTAGTGAAGTGGATTCACAACAAG	6	0.15	No Hit
TGCAAATACGCACACTGCTCCAGCTACTGTGGTTCTTGTGTCAACTGATG	6	0.15	No Hit
GCTACAAAAAACACAAACTTTAAAGGTTTACAGGAGCTGTGCCGAATGGA	6	0.15	No Hit
TGGATTGAGTCCCATTGAGCAGCCTCCATCATTTGTTTAAGCAAATGTAT	6	0.15	No Hit
TGTGTCAACTGATGTTAATTTTGCATTAGAACTCAGTGATCTCAGGCACC	6	0.15	No Hit
AGGAGCTGGTGGCTGAGGGTGGAAGAATGAAAGAAAAGAAAGATGAAAGA	6	0.15	No Hit
CACTGCCTTGTGGAATGGCCTTCTCTTCTGGGTGCCTGAGGTATCCACTT	6	0.15	No Hit
GGTCATAAGAGAATTGTAGTGTTAAAAAATGACATGAAAAACCGGCTAAG	6	0.15	No Hit
GGATTCACAACAAGCCCCCACCTCCCAACACTGACCCTTGGCTTCTTCGT	6	0.15	No Hit
CGAAAGAGTACATGGAGAACAAGAAAGCTGCTGTGGAGTTAAAGGATATA	6	0.15	No Hit
TCCCATTGAGCAGCCTCCATCATTTGTTTAAGCAAATGTATTTGTGGTTG	6	0.15	No Hit
AGTTCATTTCCGATCTACCCCCCAGGTTACCACTGAAGATTCCTTGCCAC	6	0.15	No Hit
GAGCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCCCATTTTACCAT	6	0.15	No Hit
AGAAAAAACAGAAATTCCAATACCAGTAAAGAAGGGGAACCTGTCCTGTA	6	0.15	No Hit
AAGAAACCAGAGTTTTATCTGTAATTTGAAGGCCAAGGTTGATCCTTGTT	6	0.15	No Hit
GTTGTAAAGGTTGCTGATATGGAGTCTGGCAAACAAATCCAACTGATAAC	6	0.15	No Hit
CTGGTGGCTGAGGGTGGAAGAATGAAAGAAAAGAAAGATGAAAGAATGTT	6	0.15	No Hit
TTACAAGGTTCCTTTTGTGGTTCTCTCTTTGAAAGTGTTCGCACCTCAAG	6	0.15	No Hit
TGTAAATCTGGGAAGCTCTCAATGTGAAGATGTGTGTGTCCATGTTAGTT	6	0.15	No Hit
CACTGACCCTTGGCTTCTTCGTTCTAAAAGCCCTGTGAGAAATCCTCAGC	6	0.15	No Hit
AAAAAACAGAAATTCCAATACCAGTAAAGAAGGGGAACCTGTCCTGTAAC	6	0.15	No Hit
GGTTTCAGACTATGGATATTCCAAGCTGATTGAGCTACTAGAAGCCGTGC	6	0.15	No Hit
TGCTGATGATAAACTCCGTCAGAGCCTGCGAAGGTTTGCAAATACGCACA	6	0.15	No Hit
GGTGAACAGTCACCCAGTGCCAAAGCCATGCCTGGGAAAGGGTTGCAGGC	6	0.15	No Hit
GCCTCCATCATTTGTTTAAGCAAATGTATTTGTGGTTGTTCAAGAAGCCA	6	0.15	No Hit
ACTATGTCTGTGGTTAACCAATGTGGCCTCATCCCATTTTTTAAAAAAGT	6	0.15	No Hit
TGCAGACCCACGAGGGCACTGTGCCTTTGCTGAGCTTTCCAGATTGCTAT	6	0.15	No Hit
GCAAGAAAGGCCTGCTAGCCCGCGCCTCCCTGGGATCTTCGCCGCCCGAG	6	0.15	No Hit
CACACACTGGGGCATGATTGAGTGGCAAGAGAGCCATTTTCCGGTTGACA	6	0.15	No Hit
AAGGACTTCAGCGAACTTGTATTTGATCCAGACTCTTACAAGGTTCCTTT	6	0.15	No Hit
CTTTTTCTTACTTTATTTCTTCCAGTTATTTGAAAGAACTCCATTAAGGA	6	0.15	No Hit
CTGAAGAGTCTGCCTTCTTTAGTAGAGGTTTTTATGAATGATAAGGCAGA	6	0.15	No Hit
GAAGGAGCTACAGCAGCTCCTCCAAGAAGCCTTCTCCAAGCATGGCCAGG	6	0.15	No Hit
GGCTGGTCGGCAAGAAAGGCCTGCTAGCCCGCGCCTCCCTGGGATCTTCG	5	0.125	No Hit
AAAGAAGGGGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTG	5	0.125	No Hit
TTGTCAGGTAACTGTTGCTCACATAAACGCTACCGCAAAGAATGCTGCTG	5	0.125	No Hit
CCTATACTAAATATGTAGGAGAAACACTGCAGCCCAAAACTTATGGCTAC	5	0.125	No Hit
GGAAAAGGAGACCTCGTTGTTGGCAACCAGTCTAGGCAAAGCTGCTTCTA	5	0.125	No Hit
CTGCATTCCAGGAGTGAACATTGCCACTGCTCAGAATGGCGTCAAAGTAG	5	0.125	No Hit
ACTTCAGCGAACTTGTATTTGATCCAGACTCTTACAAGGTTCCTTTTGTG	5	0.125	No Hit
AACTTTTTCTTACTTTATTTCTTCCAGTTATTTGAAAGAACTCCATTAAG	5	0.125	No Hit
TGATTAGCAAGGATGCAGTGGAAAGCCCAGCAAAAAAGCAGCCCAAAAAT	5	0.125	No Hit
CCAAGCTCTAACTTCCAGTTTGAACACAGAATTAAATTAGGATGGGGGGA	5	0.125	No Hit
AAGGACATGGTCATAAGAGAATTGTAGTGTTAAAAAATGACATGAAAAAC	5	0.125	No Hit
AAATAAGCAGAGATCGTTCAGCAGGAAGTGGATTGAGTCCCATTGAGCAG	5	0.125	No Hit
AGTGGATTCACAACAAGCCCCCACCTCCCAACACTGACCCTTGGCTTCTT	5	0.125	No Hit
TCTACACACAAAAATAGGCTTAAAAACCTTATTTCATGTCTTGACCCCAG	5	0.125	No Hit
GCCAAACATGTCCTTAAACCTGTGGGTCACACACTGGGGCATGATTGAGT	5	0.125	No Hit
CAGTGCCAAAGCCATGCCTGGGAAAGGGTTGCAGGCAAATTCTGGATCTG	5	0.125	No Hit
TGCCACTGCTCAGAATGGCGTCAAAGTAGTGAAGTGGATTCACAACAAGC	5	0.125	No Hit
GTAATTTGAAGGCCAAGGTTGATCCTTGTTCTTGTGATTTAAAAATTGGC	5	0.125	No Hit
GTTCATTTCCGATCTACCCCCCAGGTTACCACTGAAGATTCCTTGCCACA	5	0.125	No Hit
GAAGCCTTGTTACATCATGCTAACCAGCTGATCAGATTTGAAGAGTTCAT	5	0.125	No Hit
GGTTAGAGTCTGATTTTTTAAACTATGCATTAAATGCATAAATTGTAAAT	5	0.125	No Hit
GTTTGAACACAGAATTAAATTAGGATGGGGGGAAAACTGTCTGATTCTAC	5	0.125	No Hit
AGCTACTGGGAGCCATCCCACAGGTGGTCTGGATAAAAGGACATGGTCAT	5	0.125	No Hit
AGAACAAGGAAGTCATTCAAGAGCTGAATAATTGTCAGGTAACTGTTGCT	5	0.125	No Hit
CACAACTATTTGCTTGTCCCAACAAGATGATGACATGGTCATTTGTATCC	5	0.125	No Hit
GAAGAAACCAGAGTTTTATCTGTAATTTGAAGGCCAAGGTTGATCCTTGT	5	0.125	No Hit
GATAAACTCCGTCAGAGCCTGCGAAGGTTTGCAAATACGCACACTGCTCC	5	0.125	No Hit
GTGGTTGGGAAGAAATAAGCAGAGATCGTTCAGCAGGAAGTGGATTGAGT	5	0.125	No Hit
AGTGTTCGCACCTCAAGTTCATAGTCTCCTGCAGACCCACGAGGGCACTG	5	0.125	No Hit
TCTCAATGTGAAGATGTGTGTGTCCATGTTAGTTCATCTTGACGTCTTTA	5	0.125	No Hit
AGAGAATTGTAGTGTTAAAAAATGACATGAAAAACCGGCTAAGTTCATTT	5	0.125	No Hit
ACTGCCTTGTGGAATGGCCTTCTCTTCTGGGTGCCTGAGGTATCCACTTA	5	0.125	No Hit
GGAGCTACTGGGAGCCATCCCACAGGTGGTCTGGATAAAAGGACATGGTC	5	0.125	No Hit
CAGCTGAGTGGTGAAGTGCTTCAAATGGTGTGGGTTCAATATTATTTCCA	5	0.125	No Hit
AACCGCTTCAGTGCCCACACTGAAAAACACAGGTGTGAGGGAGACACTTC	5	0.125	No Hit
CTCGAACAAGGACTTCAGCGAACTTGTATTTGATCCAGACTCTTACAAGG	5	0.125	No Hit
GTGTCAACTGATGTTAATTTTGCATTAGAACTCAGTGATCTCAGGCACCG	5	0.125	No Hit
CTCAAGTTCATAGTCTCCTGCAGACCCACGAGGGCACTGTGCCTTTGCTG	5	0.125	No Hit
ACAAAAGGGATGGAAAAGGAGACCTCGTTGTTGGCAACCAGTCTAGGCAA	5	0.125	No Hit
AAATTCCAATACCAGTAAAGAAGGGGAACCTGTCCTGTAACTCCTCACCC	5	0.125	No Hit
ATCTGGGAAGCTCTCAATGTGAAGATGTGTGTGTCCATGTTAGTTCATCT	5	0.125	No Hit
CCAATACCAGTAAAGAAGGGGAACCTGTCCTGTAACTCCTCACCCAGTAG	5	0.125	No Hit
TGGGAAGCTCTCAATGTGAAGATGTGTGTGTCCATGTTAGTTCATCTTGA	5	0.125	No Hit
GGAGAAACACTGCAGCCCAAAACTTATGGCTACAGTAGTGTGGAGGAGCT	5	0.125	No Hit
CGAAAGGGCTGGTCGGCAAGAAAGGCCTGCTAGCCCGCGCCTCCCTGGGA	5	0.125	No Hit
ACCTCCGGTTTGTTTAAAAGGCTCACTTTACTGTGAAGACTGTCTAAACA	5	0.125	No Hit
CAAAAGGGATGGAAAAGGAGACCTCGTTGTTGGCAACCAGTCTAGGCAAA	5	0.125	No Hit
ATGACATGGTCATTTGTATCCGAAAAAGAGAACGAACTCAGGATGAAATA	5	0.125	No Hit
CCAGCATCTGCTTCTGCTCCTCCCAAACCCTCCTCAGAAGCCCCCGGGCT	5	0.125	No Hit
CCAACACTGACCCTTGGCTTCTTCGTTCTAAAAGCCCTGTGAGAAATCCT	5	0.125	No Hit
TCGCCGCCCGAGCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCCCA	5	0.125	No Hit
AAAAAAGTTTCCATTTGGGGATTCCATTTAATTAAGAGCTTTCTAGAAAG	5	0.125	No Hit
CACTGCCTAACAGTGGCCCAACCGCTTCAGTGCCCACACTGAAAAACACA	5	0.125	No Hit
GGCTACTGATTAGCAAGGATGCAGTGGAAAGCCCAGCAAAAAAGCAGCCC	5	0.125	No Hit
GCCTATACTAAATATGTAGGAGAAACACTGCAGCCCAAAACTTATGGCTA	5	0.125	No Hit
TCTCGATGTCATCCATTTAAGTCAGCATTTGAATGACATATTGTGTATTG	5	0.125	No Hit
CACAGAGAAGCAGAGTTTATCTGTGTGTGTGACATCAGTAAAGAGAACAA	5	0.125	No Hit
TGGAAAGCCCAGCAAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGCC	5	0.125	No Hit
GGTTAACCAATGTGGCCTCATCCCATTTTTTAAAAAAGTTTCCATTTGGG	5	0.125	No Hit
CCCACGAGGGCACTGTGCCTTTGCTGAGCTTTCCAGATTGCTATGCTGCG	5	0.125	No Hit
AGAGATTGGTGAGAAGGGTGTGAACCTGTCGGGGGGCCAGAAGCAGCGTG	5	0.125	No Hit
CTTTTATTAATGGTCCATAGGGAAATGTTTGTTTTCAACTTTCAGATGAA	5	0.125	No Hit
ATGGTCATTTGTATCCGAAAAAGAGAACGAACTCAGGATGAAATAGAAAG	5	0.125	No Hit
GGCTCCTCTACAAACTGCAGCCCAGTTCTCTTTGAAGAGTTGGAGTATCA	5	0.125	No Hit
GGGTTTCAGACTATGGATATTCCAAGCTGATTGAGCTACTAGAAGCCGTG	5	0.125	No Hit
GGATTGAGTCCCATTGAGCAGCCTCCATCATTTGTTTAAGCAAATGTATT	5	0.125	No Hit
GGAATCTGTATTATATTTACCAAATTTTTCTAGACTATTGTGAATTGTGG	5	0.125	No Hit
AGCTACTGTGGTTCTTGTGTCAACTGATGTTAATTTTGCATTAGAACTCA	5	0.125	No Hit
GATGTGTGTGTCCATGTTAGTTCATCTTGACGTCTTTAATAGTGTCTAGC	5	0.125	No Hit
CACCCTTTGAAGCACTTCACCACTCAGCTGATTTACAATTTATGCATTTA	5	0.125	No Hit
ATTGGTGTTTCTCAAAGGACTGGGATGTCACGGAATACGGTGTTTGTGAC	5	0.125	No Hit
CTTCAGCGAACTTGTATTTGATCCAGACTCTTACAAGGTTCCTTTTGTGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138-139	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR26281815 read2 length is 42-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR26281815_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	42-151
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.3415	37.0	37.0	37.0	37.0	37.0
2	36.1845	37.0	37.0	37.0	37.0	37.0
3	36.3625	37.0	37.0	37.0	37.0	37.0
4	36.334	37.0	37.0	37.0	37.0	37.0
5	36.3395	37.0	37.0	37.0	37.0	37.0
6	36.323	37.0	37.0	37.0	37.0	37.0
7	36.325	37.0	37.0	37.0	37.0	37.0
8	36.223	37.0	37.0	37.0	37.0	37.0
9	36.199	37.0	37.0	37.0	37.0	37.0
10-14	36.304199999999994	37.0	37.0	37.0	37.0	37.0
15-19	36.2551	37.0	37.0	37.0	37.0	37.0
20-24	36.272800000000004	37.0	37.0	37.0	37.0	37.0
25-29	36.2764	37.0	37.0	37.0	37.0	37.0
30-34	36.139399999999995	37.0	37.0	37.0	37.0	37.0
35-39	36.1303	37.0	37.0	37.0	37.0	37.0
40-44	36.16551167791948	37.0	37.0	37.0	37.0	37.0
45-49	36.131982995748935	37.0	37.0	37.0	37.0	37.0
50-54	36.15969450824987	37.0	37.0	37.0	37.0	37.0
55-59	36.13153975488996	37.0	37.0	37.0	37.0	37.0
60-64	36.054329023778834	37.0	37.0	37.0	37.0	37.0
65-69	36.07636333075688	37.0	37.0	37.0	37.0	37.0
70-74	36.01474969672179	37.0	37.0	37.0	37.0	37.0
75-79	36.023926087279015	37.0	37.0	37.0	37.0	37.0
80-84	36.01983344042666	37.0	37.0	37.0	37.0	37.0
85-89	36.03559992977418	37.0	37.0	37.0	37.0	37.0
90-94	35.99683621467403	37.0	37.0	37.0	37.0	37.0
95-99	35.91100971895222	37.0	37.0	37.0	37.0	37.0
100-104	35.87562872095506	37.0	37.0	37.0	37.0	37.0
105-109	35.898202598671126	37.0	37.0	37.0	37.0	37.0
110-114	35.81050398025505	37.0	37.0	37.0	37.0	37.0
115-119	35.8971723632321	37.0	37.0	37.0	37.0	37.0
120-124	35.81971419887777	37.0	37.0	37.0	37.0	37.0
125-129	35.72310345252993	37.0	37.0	37.0	37.0	37.0
130-134	35.62884105734309	37.0	37.0	37.0	37.0	37.0
135-139	35.51427684704031	37.0	37.0	37.0	37.0	37.0
140-144	35.366071959741625	37.0	37.0	37.0	34.6	37.0
145-149	35.227365196826206	37.0	37.0	37.0	27.4	37.0
150-151	35.70749395969811	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	3.0
26	8.0
27	10.0
28	15.0
29	27.0
30	47.0
31	53.0
32	80.0
33	108.0
34	162.0
35	438.0
36	2665.0
37	384.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.625	10.575	15.125	38.675
2	25.724999999999998	7.3	32.15	34.825
3	26.35	11.325000000000001	22.2	40.125
4	26.375	15.4	20.674999999999997	37.55
5	26.400000000000002	24.75	26.525	22.325
6	28.15	24.15	22.2	25.5
7	18.175	27.6	37.625	16.6
8	20.325	24.925	32.425	22.325
9	21.75	21.85	33.324999999999996	23.075000000000003
10-14	24.725	27.6	28.794999999999998	18.88
15-19	22.35	29.044999999999998	28.33	20.275000000000002
20-24	22.3	24.895	30.665	22.14
25-29	23.16	27.615000000000002	27.48	21.745
30-34	22.185	28.57	27.845	21.4
35-39	22.29	27.3	28.93	21.48
40-44	22.807280728072808	27.22772277227723	29.127912791279126	20.837083708370837
45-49	22.50562640660165	26.216554138534633	27.581895473868467	23.695923980995246
50-54	23.759255553331997	28.34200520312187	24.669801881128677	23.22893736241745
55-59	22.047047047047048	26.586586586586584	28.283283283283282	23.083083083083082
60-64	23.09619238476954	27.424849699398795	26.553106212424847	22.925851703406813
65-69	22.603633443741845	26.176854361136204	28.46532169025394	22.75419050486801
70-74	23.00578034682081	27.464186981653683	26.237748177934154	23.292284493591357
75-79	23.434587678202607	25.192685507027353	28.58798045438517	22.784746360384865
80-84	20.328697850821744	28.288242730720608	29.254108723135268	22.128950695322377
85-89	21.147155137126482	28.19279574293901	29.02169463774048	21.638354482194025
90-94	22.94336502687061	27.842083505580817	27.144481190574616	22.070070276973954
95-99	23.713599833002817	28.916605782277422	26.385554743763702	20.98423964095606
100-104	23.252730936472584	26.190476190476193	29.26079117615866	21.296001696892567
105-109	24.324324324324326	28.075675675675676	27.297297297297295	20.302702702702703
110-114	23.274227723876436	26.346604215456676	27.339132374261183	23.04003568640571
115-119	24.7848934572963	25.269965929433504	26.886874169890856	23.05826644337934
120-124	21.86464171638643	25.444464533236903	29.44615199180377	23.244741758572893
125-129	21.07469512195122	24.504573170731707	30.633892276422763	23.786839430894307
130-134	22.112742140894163	27.173403043099402	29.14404450700449	21.569810309001944
135-139	19.77146081681597	26.959159201523597	30.076885095577342	23.192494886083093
140-144	20.396621927311116	29.70894284421656	27.529784346252452	22.364650882219877
145-149	20.869495339119254	30.681452909032465	26.406300225008035	22.04275152684024
150-151	21.057094403617864	13.25607687959299	37.054833239118146	28.631995477671
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	1.0
27	4.0
28	4.5
29	3.5
30	16.0
31	28.0
32	35.5
33	48.0
34	62.0
35	83.5
36	108.0
37	119.0
38	122.0
39	129.0
40	160.5
41	194.5
42	190.5
43	177.0
44	206.5
45	217.0
46	186.5
47	187.5
48	196.5
49	199.5
50	205.5
51	197.5
52	161.0
53	135.0
54	113.5
55	85.0
56	132.5
57	149.0
58	91.0
59	51.0
60	21.5
61	7.5
62	1.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.5
70	1.0
71	0.5
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.005575689991636466
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0070531809846240655
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
40-44	1.0
45-49	1.0
50-54	1.0
55-59	3.0
60-64	6.0
65-69	6.0
70-74	7.0
75-79	12.0
80-84	40.0
85-89	39.0
90-94	28.0
95-99	59.0
100-104	63.0
105-109	98.0
110-114	113.0
115-119	149.0
120-124	154.0
125-129	170.0
130-134	159.0
135-139	165.0
140-144	156.0
145-149	457.0
150-152	2113.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.800000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	48.49246231155779	19.3
2	19.912060301507537	15.85
3	12.814070351758794	15.299999999999999
4	5.778894472361809	9.2
5	4.208542713567839	8.375
6	3.077889447236181	7.35
7	1.507537688442211	4.2
8	1.3819095477386936	4.3999999999999995
9	0.6909547738693468	2.475
>10	2.0728643216080402	12.025
>50	0.06281407035175879	1.525
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACCTCC	61	1.525	No Hit
CCCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGTC	32	0.8	No Hit
CCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGTCA	21	0.525	No Hit
CGGCAGGTTATAAACATAGAGTAGAGTGTGGCAAGGAATCTTCAGTGGTA	19	0.475	No Hit
GTCCGCAAAGGCTAGCTTTTCCCAAATGCCAACATCAGCTACCATAATAA	18	0.44999999999999996	No Hit
CCCGGCACTGCTTTCCAAAATGGTGATGGTACAAAGGGATGAAATTACTG	18	0.44999999999999996	No Hit
GTTGAGAGGAGTACTGTGAGTGGTTTCATAATGTCTCTTGAGCTCATCAA	18	0.44999999999999996	No Hit
CCTCTCAATACACAATATGTCATTCAAATGCTGACTTAAATGGATGACAT	17	0.42500000000000004	No Hit
CACCACTCAGCTGATTTACAATTTATGCATTTAATGCATAGTTTAAAAAA	17	0.42500000000000004	No Hit
GCCGGCCAAAGTGGTGGTGGTAAGAGGGAATGAACTTGTTAAAGGGCATT	17	0.42500000000000004	No Hit
CGGCCAAAGTGGTGGTGGTAAGAGGGAATGAACTTGTTAAAGGGCATTCG	17	0.42500000000000004	No Hit
GGTCAGTAGACGCTTGGAGCCCATTCCAAGAATCTGCAACACATGAGGCA	16	0.4	No Hit
GGGCTGGTGGCGCAGCAAGTCCACAACATCTTTGGAGAACTGCTTTGTCC	16	0.4	No Hit
GTAGAATCAGACAGTTTTCCCCCCATCCTAATTTAATTCTGTGTTCAAAC	16	0.4	No Hit
GGCGAGGCGGTGTTCAAAAGGTTTGGAGACATACTCCTCGGAGACCAAGA	16	0.4	No Hit
CCTGGGACTTGAGAAGTTTCAGTAAATCCTGAGTAAAGCGCTTCACCTGT	15	0.375	No Hit
CTGCAGGAGACTATGAACTTGAGGTGCGAACACTTTCAAAGAGAGAACCA	15	0.375	No Hit
CCCCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGT	14	0.35000000000000003	No Hit
TCCCTGTTCACGGATTGCAATCATGTCTGTTAATTTATATAAATCAGACA	14	0.35000000000000003	No Hit
ACCAAAATGATGTGGAAACCATGCCGGTGCCTGAGATCACTGAGTTCTAA	12	0.3	No Hit
CTCACGGATTCTCTGTACAACAGTTGTTGCTGACCGGCCAGAGGGGACTG	12	0.3	No Hit
GCCGGTTTTTCATGTCATTTTTTAACACTACAATTCTCTTATGACCATGT	12	0.3	No Hit
GGCTGCAGTGTTTCTCCTACATATTTAGTATAGGCCATGGCAAATTCATG	12	0.3	No Hit
CCCAAACATGCTTCCAACAGTGAACAGTAACAGTAAAGCTAGACACTATT	11	0.27499999999999997	No Hit
CTGCTCAATGGGACTCAATCCACTTCCTGCTGAACGATCTCTGCTTATTT	11	0.27499999999999997	No Hit
GCTGCTCAATGGGACTCAATCCACTTCCTGCTGAACGATCTCTGCTTATT	11	0.27499999999999997	No Hit
CTCCGCAGCATAGCAATCTGGAAAGCTCAGCAAAGGCACAGTGCCCTCGT	11	0.27499999999999997	No Hit
GGGGGGTAGATCGGAAATGAACTCTTCAAATCTGATCAGCTGGTTAGCAT	11	0.27499999999999997	No Hit
GTGGTAAGAGGGAATGAACTTGTTAAAGGGCATTCGGAAATGGGGCTGGT	11	0.27499999999999997	No Hit
TGCCAATTTGACTCTATTTTTGGGCTGCTTTTTTGCTGGGCTTTCCACTG	11	0.27499999999999997	No Hit
TGCCAACATCAGCTACCATAATAATAAAAACCCAAACATGCTTCCAACAG	10	0.25	No Hit
CCTTACCCTTATCAAACGGAGACAGACAGAGAAGGGAAGCATGAAATAAC	10	0.25	No Hit
TCGGAAATGAACTCTTCAAATCTGATCAGCTGGTTAGCATGATGTAACAA	10	0.25	No Hit
CTTCAAATTACAGATAAAACTCTGGTTTCTTCTTAGTGGAAATAATATTG	10	0.25	No Hit
CCCAAATGGAAACTTTTTTAAAAAATGGGATGAGGCCACATTGGTTAACC	9	0.22499999999999998	No Hit
CCCAGATTTACAGCCAATTTTTAAATCACAAGAACAAGGATCAACCTTGG	9	0.22499999999999998	No Hit
GCCCCAGTGTGTGACCCACAGGTTTAAGGACATGTTTGGCTTCTTGAACA	9	0.22499999999999998	No Hit
ACGGTCATGGCCAGGTCTCTCCAATAGTTTGTTTCTTCCTCCTCTGATCC	9	0.22499999999999998	No Hit
CCTTATCAAACGGAGACAGACAGAGAAGGGAAGCATGAAATAACTTTCTA	9	0.22499999999999998	No Hit
GCGGTGTTCAAAAGGTTTGGAGACATACTCCTCGGAGACCAAGACTGAGA	9	0.22499999999999998	No Hit
GTGCAGGAGAGCTGGCAATGCTCTGGGAACTGAGGTTCTCTTTTTTTTGA	9	0.22499999999999998	No Hit
CCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGTCAC	9	0.22499999999999998	No Hit
TGCTTTTTTGCTGGGCTTTCCACTGCATCCTTGCTAATCAGTAGCCCGGG	9	0.22499999999999998	No Hit
CTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACCTCC	9	0.22499999999999998	No Hit
TGGTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACCT	9	0.22499999999999998	No Hit
CTCTCAATACACAATATGTCATTCAAATGCTGACTTAAATGGATGACATC	8	0.2	No Hit
GCTCAATGGGACTCAATCCACTTCCTGCTGAACGATCTCTGCTTATTTCT	8	0.2	No Hit
GTGTGTGACCCACAGGTTTAAGGACATGTTTGGCTTCTTGAACAACCACA	8	0.2	No Hit
ACCCGGCACTGCTTTCCAAAATGGTGATGGTACAAAGGGATGAAATTACT	8	0.2	No Hit
GGCTGCTCAATGGGACTCAATCCACTTCCTGCTGAACGATCTCTGCTTAT	8	0.2	No Hit
GTTCTGGCACCTCCAGGATCTGCCTGCTGACTGCAGGCTGGTCTTCATGA	8	0.2	No Hit
GCCAGATTTTAGCAGCATCAATTATACTATTTCTTGCTGGCTTGTTTAGA	8	0.2	No Hit
TGGCAATGTTGAAAGCAAGTGGCGAGGCGGTGTTCAAAAGGTTTGGAGAC	8	0.2	No Hit
GCCCATTTCCCTGTTCACGGATTGCAATCATGTCTGTTAATTTATATAAA	8	0.2	No Hit
CTGCTTTTTTGCTGGGCTTTCCACTGCATCCTTGCTAATCAGTAGCCCGG	8	0.2	No Hit
AACCCAAACATGCTTCCAACAGTGAACAGTAACAGTAAAGCTAGACACTA	8	0.2	No Hit
AGCCAATTTTTAAATCACAAGAACAAGGATCAACCTTGGCCTTCAAATTA	8	0.2	No Hit
GCTGCTTTTTTGCTGGGCTTTCCACTGCATCCTTGCTAATCAGTAGCCCG	8	0.2	No Hit
GTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAGAATCAG	8	0.2	No Hit
CCACTCAGCTGATTTACAATTTATGCATTTAATGCATAGTTTAAAAAATC	8	0.2	No Hit
CTGGGGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAGA	8	0.2	No Hit
CCACAGACATAGTTACATAAAAAACAAGGCACACCAATAAGTCACCTAGA	8	0.2	No Hit
CGGCACTGCTTTCCAAAATGGTGATGGTACAAAGGGATGAAATTACTGAT	8	0.2	No Hit
ATTTGAAGCACTTCACCACTCAGCTGATTTACAATTTATGCATTTAATGC	8	0.2	No Hit
CCCCTCTCTCTTTTTCTTCTGTGCAGGAGAGCTGGCAATGCTCTGGGAAC	8	0.2	No Hit
CTCATCAACGGGAAGATAAGCATCTCCTTCCCAAGACATCAACACCACCA	8	0.2	No Hit
CGGCTGGGTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGG	8	0.2	No Hit
GGGGCATTCGGAAATGGGGCTGGTGGCGCAGCAAGTCCACAACATCTTTG	7	0.17500000000000002	No Hit
GGCTGAGCTCAACACTCTTCACCTGGCCATGCTTGGAGAAGGCTTCTTGG	7	0.17500000000000002	No Hit
ATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGTCACAGGTGCAAA	7	0.17500000000000002	No Hit
CCCACACCATTTGAAGCACTTCACCACTCAGCTGATTTACAATTTATGCA	7	0.17500000000000002	No Hit
GCAGGCTGAGTACTTGGAGGTGGTATATTTGGCCAGATTTTAGCAGCATC	7	0.17500000000000002	No Hit
CGGGTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACC	7	0.17500000000000002	No Hit
CCTCGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTTCT	7	0.17500000000000002	No Hit
GGTCACCAAACTCCGCAGCATAGCAATCTGGAAAGCTCAGCAAAGGCACA	7	0.17500000000000002	No Hit
CCCGGTTTTTCATGTCATTTTTTAACACTACAATTCTCTTATGACCATGT	7	0.17500000000000002	No Hit
GGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAGAATCA	7	0.17500000000000002	No Hit
CTGCCGGCCAAAGTGGTGGTGGTAAGAGGGAATGAACTTGTTAAAGGGCA	7	0.17500000000000002	No Hit
GCCAAAGTGGTGGTGGTAAGAGGGAATGAACTTGTTAAAGGGCATTCGGA	7	0.17500000000000002	No Hit
GGCTGGTGGCGCAGCAAGTCCACAACATCTTTGGAGAACTGCTTTGTCCT	7	0.17500000000000002	No Hit
CGGGGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAGAA	7	0.17500000000000002	No Hit
GGTGGTAAGAGGGAATGAACTTGTTAAAGGGCATTCGGAAATGGGGCTGG	7	0.17500000000000002	No Hit
TCCTTCCCAAGACATCAACACCACCAGCAACTGAACAGTGAGAGGCCGCA	7	0.17500000000000002	No Hit
GCAGCCTGTGATACTTAGCACCTTGCCACCACAGTTATCAGAGAGGCGCC	7	0.17500000000000002	No Hit
GGGGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAGAAT	7	0.17500000000000002	No Hit
CCGGCAGGTTATAAACATAGAGTAGAGTGTGGCAAGGAATCTTCAGTGGT	7	0.17500000000000002	No Hit
CCGTGACATCCCAGTCCTTTGAGAAACACCAATGATAAGCCTGTGAGAAA	7	0.17500000000000002	No Hit
GTCTTGTTCTTCAGTAGGCCCATGGGACCCACCACCTTCTCAAAGATGTG	7	0.17500000000000002	No Hit
TGCTCAATGGGACTCAATCCACTTCCTGCTGAACGATCTCTGCTTATTTC	7	0.17500000000000002	No Hit
GCTGCCAATTTGACTCTATTTTTGGGCTGCTTTTTTGCTGGGCTTTCCAC	7	0.17500000000000002	No Hit
ATTGGCAATGTTGAAAGCAAGTGGCGAGGCGGTGTTCAAAAGGTTTGGAG	7	0.17500000000000002	No Hit
CTCGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTTCTC	6	0.15	No Hit
GCAATGTTGAAAGCAAGTGGCGAGGCGGTGTTCAAAAGGTTTGGAGACAT	6	0.15	No Hit
CCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACCTCCAG	6	0.15	No Hit
CTCCTCTGATCCAAAAGTCTTTCCTGAGTCCTCCATGGTAAAATGGGCAA	6	0.15	No Hit
CGGAAATGGGGCTGGTGGCGCAGCAAGTCCACAACATCTTTGGAGAACTG	6	0.15	No Hit
GTGGGGGCTTGTTGTGAATCCACTTCACTACTTTGACGCCATTCTGAGCA	6	0.15	No Hit
GCCTGGTTACTGGGTAAGAGACACACTAGTCGCCCATTTCCCTGTTCACG	6	0.15	No Hit
CGTAATTTCTAGGTCACCAAACTCCGCAGCATAGCAATCTGGAAAGCTCA	6	0.15	No Hit
CTTTTTTGCTGGGCTTTCCACTGCATCCTTGCTAATCAGTAGCCCGGGGG	6	0.15	No Hit
CTGCTGGTAGTGATAGGTATGAAGCAAAGACCTCACATTCTTTGCAAACA	6	0.15	No Hit
CTTCTGTGAAGTGTCTCCCTCACACCTGTGTTTTTCAGTGTGGGCACTGA	6	0.15	No Hit
ACAGACATAGTTACATAAAAAACAAGGCACACCAATAAGTCACCTAGAAG	6	0.15	No Hit
CGTGCCAACAGGTTCTCTCTGCTCTATGAATGGATTTGCCCTTTTTGCCC	6	0.15	No Hit
GCCACTCAATCATGCCCCAGTGTGTGACCCACAGGTTTAAGGACATGTTT	6	0.15	No Hit
CCCTGCTTGCTGGCCTGGGACTTGAGAAGTTTCAGTAAATCCTGAGTAAA	6	0.15	No Hit
CTCCGGCACTGCTTTCCAAAATGGTGATGGTACAAAGGGATGAAATTACT	6	0.15	No Hit
GGGCTGCTTTTTTGCTGGGCTTTCCACTGCATCCTTGCTAATCAGTAGCC	6	0.15	No Hit
CCCTGTTCACGGATTGCAATCATGTCTGTTAATTTATATAAATCAGACAC	6	0.15	No Hit
GCAGACTCTTCAGTAGCTCAGTCAAAGTCATGAAACCATATTCACAGGGG	6	0.15	No Hit
GTGGGCTTGTTGTGAATCCACTTCACTACTTTGACGCCATTCTGAGCAGT	6	0.15	No Hit
CCGGTTTTTCATGTCATTTTTTAACACTACAATTCTCTTATGACCATGTC	6	0.15	No Hit
ACCCAAACATGCTTCCAACAGTGAACAGTAACAGTAAAGCTAGACACTAT	6	0.15	No Hit
ATCATGCCCCAGTGTGTGACCCACAGGTTTAAGGACATGTTTGGCTTCTT	6	0.15	No Hit
TCCTGCTCCATCTGGCTGGGTCCTGTTCTGAGATGGAGCTCTGAGGCTAG	6	0.15	No Hit
GTCTGCAGGAGACTATGAACTTGAGGTGCGAACACTTTCAAAGAGAGAAC	6	0.15	No Hit
GGGCAAGCAAATAGTTGTGTCTGGGATCTCTGATATTATATCAATCAAGT	6	0.15	No Hit
GCCCACACCATTTGAAGCACTTCACCACTCAGCTGATTTACAATTTATGC	6	0.15	No Hit
GTGGCGATGTTCACTCCTGGAATGCAGGTGATGAAGTGCTCTAAGGGACA	6	0.15	No Hit
GTATGCAAGATGGTTGGTTCTTTAGTAAATCAATGACTTCTCTGCTGAAC	6	0.15	No Hit
GAGGGAATGAACTTGTTAAAGGGCATTCGGAAATGGGGCTGGTGGCGCAG	6	0.15	No Hit
CGGCTGTTCACAGCACAGATTGCGTCATGCAGGTTTCTCATCTGCACGAT	6	0.15	No Hit
GGTTGGTTCTTTAGTAAATCAATGACTTCTCTGCTGAACTGAATCAGCTG	6	0.15	No Hit
CACCATTTGAAGCACTTCACCACTCAGCTGATTTACAATTTATGCATTTA	6	0.15	No Hit
CTTCCCAGATTTACAGCCAATTTTTAAATCACAAGAACAAGGATCAACCT	6	0.15	No Hit
CCAGAGGGGACTGAGCAGTTTTCAATATCCCAGAAAACACCAATGGGGGG	6	0.15	No Hit
GCTGGTGGCGCAGCAAGTCCACAACATCTTTGGAGAACTGCTTTGTCCTT	6	0.15	No Hit
CCTGGTTACTGGGTAAGAGACACACTAGTCGCCCATTTCCCTGTTCACGG	6	0.15	No Hit
CACATTCTTTGCAAACAAGTATAGGCTTGTGAGCTTCACACACTCTTCTG	6	0.15	No Hit
GCCCGCTCTGCGCTGTCTTGGTTTATAAAGCGGAGAATAGCGCTGCAGCC	6	0.15	No Hit
ACCGTATTCCGTGACATCCCAGTCCTTTGAGAAACACCAATGATAAGCCT	6	0.15	No Hit
GGGCCTGGTTACTGGGTAAGAGACACACTAGTCGCCCATTTCCCTGTTCA	6	0.15	No Hit
CCTGGAATGCAGGTGATGAAGTGCTCTAAGGGGACACCTTTGTCTCTGTC	6	0.15	No Hit
GCCGGCAGGTTATAAACATAGAGTAGAGTGTGGCAAGGAATCTTCAGTGG	6	0.15	No Hit
CCCTTATCAAACGGAGACAGACAGAGAAGGGAAGCATGAAATAACTTTCT	6	0.15	No Hit
GGCTTCTGGGGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGT	6	0.15	No Hit
CCTGCTTGCTGGCCTGGGACTTGAGAAGTTTCAGTAAATCCTGAGTAAAG	6	0.15	No Hit
TGGGTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACC	6	0.15	No Hit
TGGGCCGCAAAGACTTCCGGGTTATCAGTTGGATTTGTTTGCCAGACTCC	6	0.15	No Hit
TACCATAATAATAAAAACCCAAACATGCTTCCAACAGTGAACAGTAACAG	6	0.15	No Hit
TGAGAGGAGTACTGTGAGTGGTTTCATAATGTCTCTTGAGCTCATCAACG	5	0.125	No Hit
AGCACCTTGCCACCACAGTTATCAGAGAGGCGCCTGAGCCTGTTGCTGAT	5	0.125	No Hit
GTCCAAGAGGTAGATGTCAGAGTTACAATACACAGCCCGAGCCAGGCTCA	5	0.125	No Hit
TGCCTGAGATCACTGAGTTCTAATGCAAAATTAACATCAGTTGACACAAG	5	0.125	No Hit
TCCTCCTCTGATCCAAAAGTCTTTCCTGAGTCCTCCATGGTAAAATGGGC	5	0.125	No Hit
CCATAATAATAAAAACCCAAACATGCTTCCAACAGTGAACAGTAACAGTA	5	0.125	No Hit
CGGAAATGAACTCTTCAAATCTGATCAGCTGGTTAGCATGATGTAACACG	5	0.125	No Hit
GCCTGAGCCTGTTGCTGATGCTCTTGCCATCTTTATTTGCCGGCAGGTTA	5	0.125	No Hit
CCGGCACTGCTTTCCAAAATGGTGATGGTACAAAGGGATGAAATTACTGA	5	0.125	No Hit
GCTGGGTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCA	5	0.125	No Hit
GCAATGTTCACTCCTGGAATGCAGGTGATGAAGTGCTCTAAGGGGACACC	5	0.125	No Hit
GCACTGCCGGCCAAAGTGGTGGTGGTAAGAGGGAATGAACTTGTTAAAGG	5	0.125	No Hit
CTGCATCCTTGCTAATCAGTAGCCCGGGGGCTTCTGAGGAGGGTTTGGGA	5	0.125	No Hit
TCCCCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGG	5	0.125	No Hit
GGGCTGCTCAATGGGACTCAATCCACTTCCTGCTGAACGATCTCTGCTTA	5	0.125	No Hit
ACTCTCAATACACAATATGTCATTCAAATGCTGACTTAAATGGATGACAT	5	0.125	No Hit
GGCACTGCTTTCCAAAATGGTGATGGTACAAAGGGATGAAATTACTGATT	5	0.125	No Hit
GCCACCACAGTTATCAGAGAGGCGCCTGAGCCTGTTGCTGATGCTCTTGC	5	0.125	No Hit
GCTGAATGCAGATGGGTAGCTTATTTGGAAGACAGTCCCCTCTCTCTTTT	5	0.125	No Hit
CCGTATTCCGTGACATCCCAGTCCTTTGAGAAACACCAATGATAAGCCTG	5	0.125	No Hit
CTTATCAAACGGAGACAGACAGAGAAGGGAAGCATGAAATAACTTTCTAG	5	0.125	No Hit
GGAAAGGTCAAATGAACTTAGCCGGTTTTTCATGTCATTTTTTAACACTA	5	0.125	No Hit
CTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGTCACAGGTGC	5	0.125	No Hit
GCCTCTCAATACACAATATGTCATTCAAATGCTGACTTAAATGGATGACA	5	0.125	No Hit
CGGCTGCTTTTTTGCTGGGCTTTCCACTGCATCCTTGCTAATCAGTAGCC	5	0.125	No Hit
CCACACCATTTGAAGCACTTCACCACTCAGCTGATTTACAATTTATGCAT	5	0.125	No Hit
ACCAGTATCTTCTTGCTGCCAATTTTGTATCTGTGGAGGCTGTTCACAGC	5	0.125	No Hit
CCTGAGATCACTGAGTTCTAATGCAAAATTAACATCAGTTGACACAAGAA	5	0.125	No Hit
TCTGGCACCTCCAGGATCTGCCTGCTGACTGCAGGCTGGTCTTCATGATT	5	0.125	No Hit
TGGTGGCGCAGCAAGTCCACAACATCTTTGGAGAACTGCTTTGTCCTTTC	5	0.125	No Hit
GGTCTCTCCAATAGTTTGTTTCTTCCTCCTCTGATCCAAAAGTCTTTCCT	5	0.125	No Hit
GTTGGTTCTTTAGTAAATCAATGACTTCTCTGCTGAACTGAATCAGCTGA	5	0.125	No Hit
TGGACCATTAATAAAAGTGGCTTCTGGGGTCAAGACATGAAATAAGGTTT	5	0.125	No Hit
GGGCATTCGGAAATGGGGCTGGTGGCGCAGCAAGTCCACAACATCTTTGG	5	0.125	No Hit
TGGACACACACATCTTCACATTGAGAGCTTCCCAGATTTACAGCCAATTT	5	0.125	No Hit
GTCTGGATCAAATACAAGTTCGCTGAAGTCCTTGTTCGAGCAGTGCTGCT	5	0.125	No Hit
CACGAATCTGTGTGCAAGCAGCACTGCTCGAACAAGGACTTCAGCGAACT	5	0.125	No Hit
CCCTCTGCTTGTAGGTCATGGCCAGGTCTCTCCAATAGTTTGTTTCTTCC	5	0.125	No Hit
CTCCACATTCCAACACTTGTAAAATCTCAGGTATGGCTTCCAAAAGTTCC	5	0.125	No Hit
ATGGCCTTGCTGATGGTCACGGATTCTATTGCCAGACTTTGGTTCCATTC	5	0.125	No Hit
CCTGGAAGAAATAAAGTAAGAAAAAGTTCATCTGAAAGTTGGAAACAAAC	5	0.125	No Hit
CTGTGATACTTAGCACCTTGCCACCACAGTTATCAGAGAGGCGCCTGAGC	5	0.125	No Hit
CAACAACGAGGTCTCCTTTTCCATCCCTTTTGTTCCACAGCGATTACACA	5	0.125	No Hit
TGGCCATGGCAAATTCATGAAGAAAAATCTGCTGGTAGTGATAGGTATGA	5	0.125	No Hit
GGCATTCGGAAATGGGGCTGGTGGCGCAGCAAGTCCACAACATCTTTGGA	5	0.125	No Hit
GGTAGCTTATTTGGAAGACAGTCCCCTCTCTCTTTTTCTTCTGTGCAGGA	5	0.125	No Hit
GGGTAGCTTATTTGGAAGACAGTCCCCTCTCTCTTTTTCTTCTGTGCAGG	5	0.125	No Hit
TTCCTGCTGAACGATCTCTGCTTATTTCTTCCCAACCACCACAATTCACA	5	0.125	No Hit
CCCCAGTGTGTGACCCACAGGTTTAAGGACATGTTTGGCTTCTTGAACAA	5	0.125	No Hit
CTGTACTCATTGTCTATCCAATGTGCCATGTCCTGCACATAGTTACCGTT	5	0.125	No Hit
TTGCCCCAGTGTGTGACCCACAGGTTTAAGAACATGTTTGGCTTCTTGAA	5	0.125	No Hit
CCACCGTATTCCGTGACATCCCAGTCCTTTGAGAAACACCAATGATAAGC	5	0.125	No Hit
TGCAGGTTTCTCATCTGCACGATGGCTTTGAGCTGGTAATCAGTATGGGG	5	0.125	No Hit
CCCCTTATCAAACGGAGACAGACAGAGAAGGGAAGCATGAAATAACTTTC	5	0.125	No Hit
CGCTTCCCAACATGTGCATCCACAGCCGAGAGGGGGTCGTCCAAGAGGTA	5	0.125	No Hit
ACACCGTATTCCGTGACATCCCAGTCCTTTGAGAAACACCAATGATAAGC	5	0.125	No Hit
GGCCATTCCACAAGGCAGTGTCCGCAAAGGCTAGCTTTTCCCAAATGCCA	5	0.125	No Hit
CTGGTTTCTTCTTAGTGGAAATAATATTGAACCCACACCATTTGAAGCAC	5	0.125	No Hit
GCACACCAATAAGTCACCTAGAAGCCAGGAACTTTCCTGTCAACCGGAAA	5	0.125	No Hit
TGTGGCAATGTTCACTCCTGGAATGCAGGTGATGAAGTGCTCTAAGGGGA	5	0.125	No Hit
GTCATTTTTTAACACTACAATTCTCTTATGACCATGTCCTTTTATCCAGA	5	0.125	No Hit
CGGTTGGTTCTTTAGTAAATCAATGACTTCTCTGCTGAACTGAATCAGCT	5	0.125	No Hit
CTCTCTTTTTCTTCTGTGCAGGAGAGCTGGCAATGCTCTGGGAACTGAGG	5	0.125	No Hit
GCTGAGTACTTGGAGGTGGTATATTTGGCCAGATTTTAGCAGCATCAATT	5	0.125	No Hit
CCAATGTGGCCTCATCCCATTTTTTAAAAAAGTTTCCATTTGGGGATTCC	5	0.125	No Hit
ACAGGCAGGAGCATCCTGAAGAATAGACATTGTTTCTGTACTTAGTAAAG	5	0.125	No Hit
CTGAGTTCTAATGCAAAATTAACATCAGTTGACACAAGAACCACAGTAGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.1	0.0	0.0	0.0
4	0.0	0.1	0.0	0.0	0.0
5	0.0	0.1	0.0	0.0	0.0
6	0.0	0.1	0.0	0.0	0.0
7	0.0	0.1	0.0	0.0	0.0
8	0.0	0.1	0.0	0.0	0.0
9	0.0	0.125	0.0	0.0	0.0
10-11	0.0	0.125	0.0	0.0	0.0
12-13	0.0	0.125	0.0	0.0	0.0
14-15	0.0	0.1875	0.0	0.0	0.0
16-17	0.0	0.3375	0.0	0.0	0.0
18-19	0.0	0.4	0.0	0.0	0.0
20-21	0.0	0.4	0.0	0.0	0.0
22-23	0.0	0.4	0.0	0.0	0.0
24-25	0.0	0.425	0.0	0.0	0.0
26-27	0.0	0.4375	0.0	0.0	0.0
28-29	0.0	0.45	0.0	0.0	0.0
30-31	0.0	0.45	0.0	0.0	0.0
32-33	0.0	0.45	0.0	0.0	0.0
34-35	0.0	0.45	0.0	0.0	0.0
36-37	0.0	0.45	0.0	0.0	0.0
38-39	0.0	0.45	0.0	0.0	0.0
40-41	0.0	0.45	0.0	0.0	0.0
42-43	0.0	0.45	0.0	0.0	0.0
44-45	0.0	0.45	0.0	0.0	0.0
46-47	0.0	0.45	0.0	0.0	0.0
48-49	0.0	0.45	0.0	0.0	0.0
50-51	0.0	0.45	0.0	0.0	0.0
52-53	0.0	0.45	0.0	0.0	0.0
54-55	0.0	0.45	0.0	0.0	0.0
56-57	0.0	0.45	0.0	0.0	0.0
58-59	0.0	0.45	0.0	0.0	0.0
60-61	0.0	0.45	0.0	0.0	0.0
62-63	0.0	0.45	0.0	0.0	0.0
64-65	0.0	0.45	0.0	0.0	0.0
66-67	0.0	0.45	0.0	0.0	0.0
68-69	0.0	0.525	0.0	0.0	0.0
70-71	0.0	0.7749999999999999	0.0	0.0	0.0
72-73	0.0	0.825	0.0	0.0	0.0
74-75	0.0	0.825	0.0	0.0	0.0
76-77	0.0	0.825	0.0	0.0	0.0
78-79	0.0	0.825	0.0	0.0	0.0
80-81	0.0	0.825	0.0	0.0	0.0
82-83	0.0	0.875	0.0	0.0	0.0
84-85	0.0	0.925	0.0	0.0	0.0
86-87	0.0	0.925	0.0	0.0	0.0
88-89	0.0	0.925	0.0	0.0	0.0
90-91	0.0	0.925	0.0	0.0	0.0
92-93	0.0	0.975	0.0	0.0	0.0
94-95	0.0	0.975	0.0	0.0	0.0
96-97	0.0	0.975	0.0	0.0	0.0
98-99	0.0	0.975	0.0	0.0	0.0
100-101	0.0	0.975	0.0	0.0	0.0
102-103	0.0	0.975	0.0	0.0	0.0
104-105	0.0	0.975	0.0	0.0	0.0
106-107	0.0	0.975	0.0	0.0	0.0
108-109	0.0	0.975	0.0	0.0	0.0
110-111	0.0	0.975	0.0	0.0	0.0
112-113	0.0	0.975	0.0	0.0	0.0
114-115	0.0	0.975	0.0	0.0	0.0
116-117	0.0	0.975	0.0	0.0	0.0
118-119	0.0	0.975	0.0	0.0	0.0
120-121	0.0	0.975	0.0	0.0	0.0
122-123	0.0	0.975	0.0	0.0	0.0
124-125	0.0	0.975	0.0	0.0	0.0
126-127	0.0	0.975	0.0	0.0	0.0
128-129	0.0	0.975	0.0	0.0	0.0
130-131	0.0	0.975	0.0	0.0	0.0
132-133	0.0	0.975	0.0	0.0	0.0
134-135	0.0	0.975	0.0	0.0	0.0
136-137	0.0	0.975	0.0	0.0	0.0
138-139	0.0	0.975	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 9344253 spots for SRR26281815.sra
Written 9344253 spots for SRR26281815.sra
Read 9344253 spots for SRR26281815.sra
Written 9344253 spots for SRR26281815.sra
Read 9344253 spots for SRR26281815.sra
Written 9344253 spots for SRR26281815.sra
Read 9344253 spots for SRR26281815.sra
Written 9344253 spots for SRR26281815.sra
Read 9344253 spots for SRR26281815.sra
Written 9344253 spots for SRR26281815.sra
Read 9344253 spots for SRR26281815.sra
Written 9344253 spots for SRR26281815.sra
Read 9344253 spots for SRR26281815.sra
Written 9344253 spots for SRR26281815.sra
Rejected 19449 READS because READLEN < 1
Read 9344258 spots for SRR26281815.sra
Written 9344258 spots for SRR26281815.sra
SRR ids: ['SRR26281815.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_aa7ht333
SRR26281815.sra spots: 74754029
blocks: [[1, 9344253], [9344254, 18688506], [18688507, 28032759], [28032760, 37377012], [37377013, 46721265], [46721266, 56065518], [56065519, 65409771], [65409772, 74754029]]
SRR26281815 file size 23675304
SRR26281815 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR26281815 SRR26281815_1.fastq SRR26281815_2.fastq
Input file:	SRR26281815_1.fastq
Paired file:	SRR26281815_2.fastq
trimmed:	SRR26281815-trimmed-pair1.fastq, SRR26281815-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Mon Nov 10 21:27:09 2025 >> started

Mon Nov 10 21:32:53 2025 >> done (343.597s)
74737392 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
74737392 (100.00%) read pairs available; of these:
  189944 ( 0.25%) trimmed read pairs available after processing
74547448 (99.75%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 35	    1044	  0.00%
 36	    1203	  0.00%
 37	    1498	  0.00%
 38	    1846	  0.00%
 39	    2342	  0.00%
 40	    2776	  0.00%
 41	    3255	  0.00%
 42	    3443	  0.00%
 43	    3468	  0.00%
 44	    3243	  0.00%
 45	    3189	  0.00%
 46	    3665	  0.00%
 47	    4289	  0.01%
 48	    5067	  0.01%
 49	    6223	  0.01%
 50	    7104	  0.01%
 51	    8121	  0.01%
 52	    8830	  0.01%
 53	    8747	  0.01%
 54	    8647	  0.01%
 55	    8984	  0.01%
 56	    9286	  0.01%
 57	   10420	  0.01%
 58	   11719	  0.02%
 59	   13631	  0.02%
 60	   15259	  0.02%
 61	   17512	  0.02%
 62	   19178	  0.03%
 63	   20711	  0.03%
 64	   21984	  0.03%
 65	   22610	  0.03%
 66	   23330	  0.03%
 67	   23903	  0.03%
 68	   25102	  0.03%
 69	   27645	  0.04%
 70	   30009	  0.04%
 71	   33738	  0.05%
 72	   39240	  0.05%
 73	   44684	  0.06%
 74	   49541	  0.07%
 75	   53897	  0.07%
 76	   56046	  0.07%
 77	   57538	  0.08%
 78	   59449	  0.08%
 79	   61587	  0.08%
 80	   66605	  0.09%
 81	   73354	  0.10%
 82	   82668	  0.11%
 83	   94007	  0.13%
 84	  106610	  0.14%
 85	  118332	  0.16%
 86	  124671	  0.17%
 87	  130827	  0.18%
 88	  133329	  0.18%
 89	  135241	  0.18%
 90	  141097	  0.19%
 91	  151419	  0.20%
 92	  164938	  0.22%
 93	  184524	  0.25%
 94	  205630	  0.28%
 95	  227272	  0.30%
 96	  243797	  0.33%
 97	  254493	  0.34%
 98	  260661	  0.35%
 99	  262978	  0.35%
100	  268933	  0.36%
101	  274659	  0.37%
102	  291288	  0.39%
103	  310381	  0.42%
104	  334429	  0.45%
105	  362236	  0.48%
106	  385683	  0.52%
107	  396511	  0.53%
108	  403148	  0.54%
109	  406579	  0.54%
110	  404190	  0.54%
111	  408321	  0.55%
112	  417980	  0.56%
113	  428886	  0.57%
114	  446115	  0.60%
115	  469561	  0.63%
116	  487704	  0.65%
117	  499856	  0.67%
118	  511855	  0.68%
119	  513398	  0.69%
120	  508799	  0.68%
121	  507314	  0.68%
122	  505127	  0.68%
123	  505651	  0.68%
124	  515142	  0.69%
125	  524350	  0.70%
126	  533415	  0.71%
127	  551252	  0.74%
128	  555733	  0.74%
129	  560132	  0.75%
130	  560713	  0.75%
131	  551605	  0.74%
132	  543324	  0.73%
133	  538006	  0.72%
134	  536735	  0.72%
135	  538934	  0.72%
136	  545881	  0.73%
137	  556422	  0.74%
138	  558674	  0.75%
139	  562501	  0.75%
140	  558887	  0.75%
141	  553437	  0.74%
142	  537794	  0.72%
143	  531600	  0.71%
144	  525056	  0.70%
145	  528687	  0.71%
146	  559267	  0.75%
147	  618499	  0.83%
148	 1191331	  1.59%
149	 5280562	  7.07%
150	21982929	 29.41%
151	19080464	 25.53%
74737392 reads passed initial QC


criterion=sequence-density
sequence-density=0.95
sequence-density-rank=1
fanout-score=2.21
fanout-score-rank=36
prefix-density=0.97
prefix-fanout=2.2
sequence=CGAGACAAGACCAT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=40
fanout-score=105.66
fanout-score-rank=1
prefix-density=0.12
prefix-fanout=5.3
sequence=AAAACAAAAAACAAAAAAAACAGAAAGAAAGAAACCAGATCCCAAGTCACAGCATTTTCCCACGTAACTCGACTCTGAGGCCATAGCCTATCCACAGCCTCCTCGTCCCCTGCACCGCCCAGTGTCTCACTGGCTGTGTTGGAAACGGGAATTGCATAAGCTTGCCTTCCTCAAGCAAGAAATATCTCTAGCTTTCATTTCCATTTTGACTCTTAACACTCACCCAGACTCTGTGCTTATTTCATTTGGGGGGGGGTGTGGGCTTCCTGGGGTCTTCCCCTGGTAGTTTGGAGGTAGGCAGAGGGAAGTTACAGACACAGATACAAAACTTGGGCAAGGACGCTGTGAGGCCAGTCAGAACCAGATGGCAAGTCTTGGTAGCCTAGGTCAACGACTGACAGAATAATCCAGAGCTCTGATGCACAAAACAGACTCCCAGCAGCCCGGGACCTTGCTGTCTCCTCCACTCTTCAGGCAGTTTCTTTCCATGTTTGGCTGTTGGTTTTAATTT


criterion=sequence-density
sequence-density=1.09
sequence-density-rank=1
fanout-score=2.10
fanout-score-rank=35
prefix-density=1.10
prefix-fanout=2.1
sequence=ATGGTCTTGTCTCG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=36
fanout-score=57.64
fanout-score-rank=1
prefix-density=0.12
prefix-fanout=4.6
sequence=AGGGTCCACAGGTCCAGGCTGCAGAGCGGCAGCAGGAAGCAGACCCTCCAATCTAGAACAGGGTAACCTGGTAATAAAATCAGCCCCCTAATGCCGCTCTGACCTCTGAGACGCCACACGCTGCCTACAACAACTAGAGCTCTGGAATAGTAAACAGGAGAGTGATTTCCGGGGGAAATTTTAAATAAGATGCACATGGGACAGGCCATAAAAGTTTGCCAAGTTAAATTTGGTACATAATTGTGTTCACTCCGCGTCCAAACAAAGCGTGTGCGGTCGTCACTCGGCCGCTCCTGCTGCCTCTCAGTATGGCTTGTAGTTATTCTGATGACCACCACGTCGCTGGCTCTTCCCGTAATTTGTACTACCCTGACCTCCACCACTGCCTCGGTTCCCTCGATTGCGATTTCCTCGGCCCCCAGAGCCATACTGCTGTTGCTGGTACACCTCTTTGGGCTGGGCAACCTTGATTTCACACTTGCTACCACTGACAGTATGGAATTTTTTCTCC
SRR26281815 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Nov 10 21:34:28
                             Started mapping on |	Nov 10 21:34:28
                                    Finished on |	Nov 10 21:46:09
       Mapping speed, Million of reads per hour |	383.82

                          Number of input reads |	74737392
                      Average input read length |	278
                                    UNIQUE READS:
                   Uniquely mapped reads number |	68443079
                        Uniquely mapped reads % |	91.58%
                          Average mapped length |	277.16
                       Number of splices: Total |	78383616
            Number of splices: Annotated (sjdb) |	76199849
                       Number of splices: GT/AG |	77402932
                       Number of splices: GC/AG |	543540
                       Number of splices: AT/AC |	53891
               Number of splices: Non-canonical |	383253
                      Mismatch rate per base, % |	0.43%
                         Deletion rate per base |	0.03%
                        Deletion average length |	1.73
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.75
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	6281495
             % of reads mapped to multiple loci |	8.40%
        Number of reads mapped to too many loci |	6576
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.01%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	12854	12854	12854
N_multimapping	6281495	6281495	6281495
N_noFeature	8247500	9279848	66863830
N_ambiguous	1068348	514179	10950
UnstrandedReadsAssigned:59127231 PositiveStrandReadsAssigned:58649052 NegativeStrandReadsAssigned:1568299
Dataset is classified positive stranded
MeadianReadLen=150 20thPercentileLength=126 echo kmer=121
SRR26281815 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 31,715
[index] number of k-mers: 49,915,185
[index] number of equivalence classes: 70,351
[quant] running in paired-end mode
[quant] will process pair 1: SRR26281815-trimmed-pair1.fastq
                             SRR26281815-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 74,737,392 reads, 62,915,080 reads pseudoaligned
[quant] estimated average fragment length: 187.788
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 966 rounds

  31716 SRR26281815.ke.tsv
  32883 SRR26281815.se.tsv
  64599 total
==> SRR26281815.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	768.212	79126	2459.33
ENSRNOT00000040993.4	1039	852.212	132269	3705.87
ENSRNOT00000050156.3	1545	1358.21	835826	14693.6
ENSRNOT00000043693.3	684	497.615	176618	8474.64
ENSRNOT00000046201.3	204	60.5538	38552.6	15201.7
ENSRNOT00000046108.3	681	494.683	200317	9668.77
ENSRNOT00000049683.3	784	597.356	273473	10931
ENSRNOT00000041241.3	348	172.839	18772	2593.27
ENSRNOT00000044582.3	297	129.149	15789.9	2919.22

==> SRR26281815.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	115
ENSRNOG00000058808	0
ENSRNOG00000061316	3849
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	44
SRR26281815 completed mapping pipeline successfully
