Starting /dee2/code/volunteer_pipeline.sh SRR26281820
    current disk space = 717920276
    free memory = 197265464 
SRR26281820 SRAfilesize
012af6ed2f2fb853f7fc583eca6662a5  SRR26281820.sra
SRR26281820.sra file validated
SRR26281820 is paired end
SRR26281820 is conventional basespace
SRR26281820 read1 length is 46-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR26281820_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	46-151
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.2215	37.0	37.0	37.0	37.0	37.0
2	36.204	37.0	37.0	37.0	37.0	37.0
3	36.2495	37.0	37.0	37.0	37.0	37.0
4	36.3785	37.0	37.0	37.0	37.0	37.0
5	36.381	37.0	37.0	37.0	37.0	37.0
6	36.441	37.0	37.0	37.0	37.0	37.0
7	36.22	37.0	37.0	37.0	37.0	37.0
8	36.33	37.0	37.0	37.0	37.0	37.0
9	36.326	37.0	37.0	37.0	37.0	37.0
10-14	36.3673	37.0	37.0	37.0	37.0	37.0
15-19	36.348400000000005	37.0	37.0	37.0	37.0	37.0
20-24	36.36130000000001	37.0	37.0	37.0	37.0	37.0
25-29	36.221399999999996	37.0	37.0	37.0	37.0	37.0
30-34	36.1968	37.0	37.0	37.0	37.0	37.0
35-39	36.227199999999996	37.0	37.0	37.0	37.0	37.0
40-44	36.1635	37.0	37.0	37.0	37.0	37.0
45-49	36.11656324081021	37.0	37.0	37.0	37.0	37.0
50-54	36.0997247935952	37.0	37.0	37.0	37.0	37.0
55-59	36.044867300951424	37.0	37.0	37.0	37.0	37.0
60-64	36.071515738421056	37.0	37.0	37.0	37.0	37.0
65-69	36.11144866224121	37.0	37.0	37.0	37.0	37.0
70-74	36.03850659220144	37.0	37.0	37.0	37.0	37.0
75-79	36.04280373101451	37.0	37.0	37.0	37.0	37.0
80-84	36.02060522436472	37.0	37.0	37.0	37.0	37.0
85-89	35.98305735907906	37.0	37.0	37.0	37.0	37.0
90-94	35.9440032144583	37.0	37.0	37.0	37.0	37.0
95-99	35.93379111097046	37.0	37.0	37.0	37.0	37.0
100-104	35.88136706632745	37.0	37.0	37.0	37.0	37.0
105-109	35.83881653475233	37.0	37.0	37.0	37.0	37.0
110-114	35.797922629441885	37.0	37.0	37.0	37.0	37.0
115-119	35.73461190472226	37.0	37.0	37.0	37.0	37.0
120-124	35.797794955561535	37.0	37.0	37.0	37.0	37.0
125-129	35.594041873879235	37.0	37.0	37.0	37.0	37.0
130-134	35.710854748673185	37.0	37.0	37.0	37.0	37.0
135-139	35.67246401238941	37.0	37.0	37.0	37.0	37.0
140-144	35.42893489546254	37.0	37.0	37.0	37.0	37.0
145-149	35.48657998756274	37.0	37.0	37.0	37.0	37.0
150-151	35.83192683508146	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
26	6.0
27	6.0
28	21.0
29	41.0
30	48.0
31	70.0
32	82.0
33	121.0
34	170.0
35	371.0
36	2536.0
37	528.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.25	25.424999999999997	12.775	29.549999999999997
2	29.07268170426065	22.230576441102755	28.471177944862152	20.225563909774436
3	23.3	25.174999999999997	29.799999999999997	21.725
4	21.15	32.324999999999996	24.95	21.575
5	23.375	33.7	25.374999999999996	17.549999999999997
6	19.3	35.775	23.674999999999997	21.25
7	19.25	20.849999999999998	37.075	22.825
8	23.3	22.45	28.7	25.55
9	22.025	21.7	31.3	24.975
10-14	23.14	26.51	27.325	23.025000000000002
15-19	22.915	26.995	27.825	22.264999999999997
20-24	22.495	27.589999999999996	26.58	23.335
25-29	21.98	29.28	25.025	23.715
30-34	22.900000000000002	29.035	27.005000000000003	21.060000000000002
35-39	23.799999999999997	26.655	27.71	21.834999999999997
40-44	22.5	27.029999999999998	28.970000000000002	21.5
45-49	22.1333199979997	27.914187128069212	28.004200630094516	21.948292243836576
50-54	22.376782586940205	28.246184638478862	27.910933199899922	21.46609957468101
55-59	23.670505758637958	27.356034051076616	28.012018027040558	20.961442163244868
60-64	22.190504807692307	26.91806891025641	29.081530448717945	21.809895833333336
65-69	22.11639681187027	27.815930623088875	27.359767406887563	22.70790515815329
70-74	23.473800663783567	28.89972845217741	26.87317710952429	20.753293774514734
75-79	22.76447408305547	27.836718197433562	26.85662321915732	22.542184500353642
80-84	22.866737902610286	29.09988296952119	27.614104716837122	20.41927441103139
85-89	22.28096289072525	28.450443976800287	28.999640712415953	20.26895242005851
90-94	22.36678415923699	28.384822724445364	28.11528094546962	21.13311217084802
95-99	22.498292798235017	28.686242580238485	27.183905027052578	21.63155959447392
100-104	22.72116882419294	28.119460707955096	27.856260407154753	21.30311006069721
105-109	23.81214557066564	27.732202829928976	27.34680394208005	21.108847657325335
110-114	22.9907176816844	29.097803939325335	25.713153724247228	22.198324654743036
115-119	21.89457510932514	27.02399243588228	28.418626639877083	22.662805814915497
120-124	22.73153063136202	30.075563604571286	26.04758633610192	21.14531942796478
125-129	22.433584781895703	28.2846835027878	26.24467038373237	23.037061331584127
130-134	22.059651097355093	28.84074282498593	26.350590883511536	22.74901519414744
135-139	24.163929531203344	27.694834278889218	26.194386383995223	21.946849805912212
140-144	23.14829253079682	27.428660533291747	26.843910806174957	22.579136129736472
145-149	24.53224189776144	26.896090878717004	26.31139325091881	22.26027397260274
150-151	20.438388625592417	12.52962085308057	35.24881516587678	31.78317535545024
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	2.0
25	15.0
26	21.0
27	15.0
28	17.5
29	27.0
30	22.0
31	20.0
32	33.5
33	38.0
34	41.5
35	75.0
36	105.5
37	103.0
38	96.5
39	120.5
40	166.5
41	195.0
42	215.0
43	246.5
44	284.5
45	275.5
46	251.0
47	215.5
48	196.5
49	209.0
50	195.0
51	163.5
52	127.0
53	120.0
54	98.5
55	60.0
56	49.5
57	42.5
58	58.0
59	48.0
60	25.0
61	22.5
62	10.0
63	6.0
64	9.0
65	8.0
66	0.5
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.25
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
45-49	3.0
50-54	3.0
55-59	0.0
60-64	1.0
65-69	12.0
70-74	12.0
75-79	24.0
80-84	36.0
85-89	35.0
90-94	34.0
95-99	68.0
100-104	103.0
105-109	90.0
110-114	132.0
115-119	155.0
120-124	177.0
125-129	180.0
130-134	213.0
135-139	109.0
140-144	123.0
145-149	471.0
150-152	2019.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.425000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	37.8969654199012	13.425
2	20.81863091037403	14.75
3	16.019760056457304	17.025000000000002
4	8.892025405786875	12.6
5	5.363443895553988	9.5
6	3.5991531404375445	7.6499999999999995
7	2.117148906139732	5.25
8	1.6231474947071278	4.6
9	0.776287932251235	2.475
>10	2.8934368383909668	12.725
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCTTCTG	26	0.65	No Hit
GTGTGAGTTGCCTCTTTTGCAGACGTCCTAAGGCTAAGCTTGCCTGCTCA	22	0.5499999999999999	No Hit
CAAAACTTATGGCTACAGTAGTGTGGAGGAGCTACTGGGAGCCATCCCAC	19	0.475	No Hit
GGGAGACACTTCACAGAAGCAGTCAAAAAAAAGAGAACCTCAGTTCCCAG	18	0.44999999999999996	No Hit
GTGGAAAGCCCAGCAAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGC	15	0.375	No Hit
GTTCATTTCCGATCTACCCCCCAGGTTACCACTGAAGATTCCTTGCCACA	14	0.35000000000000003	No Hit
GGGGACTGTCTTCCAAATAAGCTACCCATCTGCATTCAGCCAGCTGATCG	14	0.35000000000000003	No Hit
GTCAGTTCAGTCTCAGCTTTGACACAGAAGCTCTGCCATGTTGTAAAGGT	13	0.325	No Hit
GTGAACATTGCCACTGCTCAGAATGGCGTCAAAGTAGTGAAGTGGATTCA	13	0.325	No Hit
GGTCATTTGTATCCGAAAAAGAGAACGAACTCAGGATGAAATAGAAAGGA	13	0.325	No Hit
CAACTCTCTCCACCTAAATGTGGCACCTCCGGTTTGTTTAAAAGGCTCAC	13	0.325	No Hit
TCTCGATGTCATCCATTTAAGTCAGCATTTGAATGACATATTGTGTATTG	13	0.325	No Hit
CCAACTCTCTCCACCTAAATGTGGCACCTCCGGTTTGTTTAAAAGGCTCA	13	0.325	No Hit
CTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCTTCTGCTCCTCC	13	0.325	No Hit
TCGGAATGTAAGCGGAGAAGGAGCTGGTGGCTGAGGGTGGAAGAATGAAA	13	0.325	No Hit
GAAAAAACAGAAATTCCAATACCAGTAAAGAAGGGGAACCTGTCCTGTAA	12	0.3	No Hit
AGGGGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCTTCT	12	0.3	No Hit
CGAAAAAGAGAACGAACTCAGGATGAAATAGAAAGGACAAAGCAGTTCTC	12	0.3	No Hit
CCTAAGGCTAAGCTTGCCTGCTCAGACTAAAGGTTTGCAAGCATAGATGG	11	0.27499999999999997	No Hit
AGCATAGATGGGACTATGGACTTTGAAAACACTAGGTTTCACTAAAGTTA	11	0.27499999999999997	No Hit
TCTGCATTCAGCCAGCTGATCGCCTCAAGGCAAGTCAGTCCTCTGTTCAG	11	0.27499999999999997	No Hit
CCTCCCTGGGATCTTCGCCGCCCGAGCTGGACATCATGCCACAGGAAGAG	11	0.27499999999999997	No Hit
CCTCTCACCGCTGTTGGAGAGACCTGACATTTACAATGGATTGTATCTGT	11	0.27499999999999997	No Hit
AGGAGCTGGTGGCTGAGGGTGGAAGAATGAAAGAAAAGAAAGATGAAAGA	11	0.27499999999999997	No Hit
GTCTCCTGCAGACCCACGAGGGCACTGTGCCTTTGCTGAGCTTTCCAGAT	11	0.27499999999999997	No Hit
GGTTTACAGGAGCTGTGCCGAATGGAACCAAAGTCTGGCAATAGAATCCG	11	0.27499999999999997	No Hit
CTCCGTCAGAGCCTGCGAAGGTTTGCAAATACGCACACTGCTCCAGCTAC	11	0.27499999999999997	No Hit
ACAAGATGATGACATGGTCATTTGTATCCGAAAAAGAGAACGAACTCAGG	11	0.27499999999999997	No Hit
CGTCAAAGTAGTGAAGTGGATTCACAACAAGCCCCCACCTCCCAACACTG	11	0.27499999999999997	No Hit
TGTCCTTAAACCTGTGGGTCACACACTGGGGCATGATTGAGTGGCAAGAG	10	0.25	No Hit
TAAATATGTAGGAGAAACACTGCAGCCCAAAACTTATGGCTACAGTAGTG	10	0.25	No Hit
CGTTCAGCAGGAAGTGGATTGAGTCCCATTGAGCAGCCTCCATCATTTGT	10	0.25	No Hit
GCAGATTCTTGGAATGGGCTCCAAGCGTCTACTGACCCTCACTCATAGGG	10	0.25	No Hit
AGAAGGGGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCT	10	0.25	No Hit
CAACTATTTGCTTGTCCCAACAAGATGATGACATGGTCATTTGTATCCGA	10	0.25	No Hit
AAGAAATAAGCAGAGATCGTTCAGCAGGAAGTGGATTGAGTCCCATTGAG	10	0.25	No Hit
GAAACAATGTCTATTCTTCAGGATGCTCCTGCCTGTTGTTTGCCTCTCTT	10	0.25	No Hit
CCGAAAAAGAGAACGAACTCAGGATGAAATAGAAAGGACAAAGCAGTTCT	10	0.25	No Hit
CCTCCCAACACTGACCCTTGGCTTCTTCGTTCTAAAAGCCCTGTGAGAAA	10	0.25	No Hit
AGCTGATCAGATTTGAAGAGTTCATTTCCGATCTACCCCCCAGGTTACCA	10	0.25	No Hit
GGAGGAGCTACTGGGAGCCATCCCACAGGTGGTCTGGATAAAAGGACATG	10	0.25	No Hit
AATGTGGCCTCATCCCATTTTTTAAAAAAGTTTCCATTTGGGGATTCCAT	9	0.22499999999999998	No Hit
AGAACCTGTTGGCACACAGTCTTCCACTAGTCAGATGACTTCTTTCGAGG	9	0.22499999999999998	No Hit
CACGAATCTGTGTGCAAGCAGCACTGCTCGAACAAGGACTTCAGCGAACT	9	0.22499999999999998	No Hit
GGATATTCCAAGCTGATTGAGCTACTAGAAGCCGTGCCTCATGTGTTGCA	9	0.22499999999999998	No Hit
AGGAGCTACTGGGAGCCATCCCACAGGTGGTCTGGATAAAAGGACATGGT	9	0.22499999999999998	No Hit
ACACTGCCTTGTGGAATGGCCTTCTCTTCTGGGTGCCTGAGGTATCCACT	9	0.22499999999999998	No Hit
AGCAGAGAGAACCTGTTGGCACACAGTCTTCCACTAGTCAGATGACTTCT	9	0.22499999999999998	No Hit
ACTGAAGATTCCTTGCCACACTCTACTCTATGTTTATAACCTGCCGGCAA	9	0.22499999999999998	No Hit
CGAGCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCCCATTTTACCA	9	0.22499999999999998	No Hit
AGAAAATGGATTCTTATTTCATGGCCAGCATTTTGATAAAAATGTCACAA	9	0.22499999999999998	No Hit
CTGAAACTTCTCAAGTCCCAGGCCAGCAAGCAGGTCATTGTGAGCGATTT	9	0.22499999999999998	No Hit
GGAAAAGAGATGGTCTCGATGTCATCCATTTAAGTCAGCATTTGAATGAC	8	0.2	No Hit
GTAGTGTTAAAAAATGACATGAAAAACCGGCTAAGTTCATTTGACCTTTC	8	0.2	No Hit
CCCCAGGTTACCACTGAAGATTCCTTGCCACACTCTACTCTATGTTTATA	8	0.2	No Hit
TTGCCTGCTCAGACTAAAGGTTTGCAAGCATAGATGGGACTATGGACTTT	8	0.2	No Hit
GGTGTGGGTTCAATATTATTTCCACTAAGAAGAAACCAGAGTTTTATCTG	8	0.2	No Hit
CAAGAAAGGCCTGCTAGCCCGCGCCTCCCTGGGATCTTCGCCGCCCGAGC	8	0.2	No Hit
AGTGGATTCACAACAAGCCCCCACCTCCCAACACTGACCCTTGGCTTCTT	8	0.2	No Hit
CTGATTAGCAAGGATGCAGTGGAAAGCCCAGCAAAAAAGCAGCCCAAAAA	8	0.2	No Hit
GAGGTATCGGAATGTAAGCGGAGAAGGAGCTGGTGGCTGAGGGTGGAAGA	8	0.2	No Hit
CGCCGCCCGAGCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCCCAT	8	0.2	No Hit
AGAAAGGCCTGCTAGCCCGCGCCTCCCTGGGATCTTCGCCGCCCGAGCTG	8	0.2	No Hit
AGCATCTGCTTCTGCTCCTCCCAAACCCTCCTCAGAAGCCCCCGGGCTAC	8	0.2	No Hit
TACAAGTGTTGGAATGTGGAGAAGAGAAAATCCTGACTTTGACTGAAGTG	8	0.2	No Hit
CTTCTCTGTCTGTCTCCGTTTGATAAGGGTAAGGTTTAAGGAAAAGAGAT	8	0.2	No Hit
CATTTACAATGGATTGTATCTGTTGGGCAAAAAGGGCAAATCCATTCATA	8	0.2	No Hit
CCCCGGGCTACTGATTAGCAAGGATGCAGTGGAAAGCCCAGCAAAAAAGC	8	0.2	No Hit
CTTCGCCGCCCGAGCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCC	8	0.2	No Hit
CTCTCCACAGGAGCTGCCAACAAGTCACTCTCTTTACTAAGTACAGAAAC	8	0.2	No Hit
GTCTGATTTTTTAAACTATGCATTAAATGCATAAATTGTAAATCAGCTGA	8	0.2	No Hit
ACTATTTGCTTGTCCCAACAAGATGATGACATGGTCATTTGTATCCGAAA	8	0.2	No Hit
GGATTGAGTCCCATTGAGCAGCCTCCATCATTTGTTTAAGCAAATGTATT	8	0.2	No Hit
GGCTAAGCTTGCCTGCTCAGACTAAAGGTTTGCAAGCATAGATGGGACTA	8	0.2	No Hit
CGGACACTGCCTTGTGGAATGGCCTTCTCTTCTGGGTGCCTGAGGTATCC	8	0.2	No Hit
GTCTCCGTTTGATAAGGGTAAGGTTTAAGGAAAAGAGATGGTCTCGATGT	7	0.17500000000000002	No Hit
GGTCGGCAAGAAAGGCCTGCTAGCCCGCGCCTCCCTGGGATCTTCGCCGC	7	0.17500000000000002	No Hit
TGTTAGTTCATCTTGACGTCTTTAATAGTGTCTAGCTTTACTGTTACTGT	7	0.17500000000000002	No Hit
GCATGTTTGGGTTTTTATTATTATGGTAGCTGATGTTGGCATTTGGGAAA	7	0.17500000000000002	No Hit
ATGTTGTAAAGGTTGCTGATATGGAGTCTGGCAAACAAATCCAACTGATA	7	0.17500000000000002	No Hit
AAGTGCTTCAAATGGTGTGGGTTCAATATTATTTCCACTAAGAAGAAACC	7	0.17500000000000002	No Hit
ACTGCTCAGAATGGCGTCAAAGTAGTGAAGTGGATTCACAACAAGCCCCC	7	0.17500000000000002	No Hit
AGAAATTCCAATACCAGTAAAGAAGGGGAACCTGTCCTGTAACTCCTCAC	7	0.17500000000000002	No Hit
AAAAAAGAGAACCTCAGTTCCCAGAGCATTGCCAGCTCTCCTGCACAGAA	7	0.17500000000000002	No Hit
GGGTTGCAGGCAAATTCTGGATCTGCTACAAAAAACACAAACTTTAAAGG	7	0.17500000000000002	No Hit
CCTGGGTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCA	7	0.17500000000000002	No Hit
GGAGAAACACTGCAGCCCAAAACTTATGGCTACAGTAGTGTGGAGGAGCT	7	0.17500000000000002	No Hit
AGAGTTCATTTCCGATCTACCCCCCAGGTTACCACTGAAGATTCCTTGCC	7	0.17500000000000002	No Hit
TGCTTCTGCTCCTCCCAAACCCTCCTCAGAAGCCCCCGGGCTACTGATTA	7	0.17500000000000002	No Hit
AGTGGATTGAGTCCCATTGAGCAGCCTCCATCATTTGTTTAAGCAAATGT	7	0.17500000000000002	No Hit
ATTTTTTCTAGAAAGCCAGAAAATGGATTCTTATTTCATGGCCAGCATTT	7	0.17500000000000002	No Hit
AATCTGGGAAGCTCTCAATGTGAAGATGTGTGTGTCCATGTTAGTTCATC	7	0.17500000000000002	No Hit
CACTGCCTTGTGGAATGGCCTTCTCTTCTGGGTGCCTGAGGTATCCACTT	7	0.17500000000000002	No Hit
CAAGTTCATAGTCTCCTGCAGACCCACGAGGGCACTGTGCCTTTGCTGAG	7	0.17500000000000002	No Hit
GCCTATACTAAATATGTAGGAGAAACACTGCAGCCCAAAACTTATGGCTA	7	0.17500000000000002	No Hit
AGTTATTTCATGCTTCCCTTCTCTGTCTGTCTCCGTTTGATAAGGGTAAG	7	0.17500000000000002	No Hit
TGAAGATTCCTTGCCACACTCTACTCTATGTTTATAACCTGCCGGCAAAT	7	0.17500000000000002	No Hit
AGAGAACCTGTTGGCACACAGTCTTCCACTAGTCAGATGACTTCTTTCGA	7	0.17500000000000002	No Hit
CCAATGTGGCCTCATCCCATTTTTTAAAAAAGTTTCCATTTGGGGATTCC	7	0.17500000000000002	No Hit
GTCAAAGTAGTGAAGTGGATTCACAACAAGCCCCCACCTCCCAACACTGA	7	0.17500000000000002	No Hit
GTAGTGTGGAGGAGCTACTGGGAGCCATCCCACAGGTGGTCTGGATAAAA	7	0.17500000000000002	No Hit
GGCAAATCCATTCATAGAGCAGAGAGAACCTGTTGGCACACAGTCTTCCA	7	0.17500000000000002	No Hit
AAACACTGCAGCCCAAAACTTATGGCTACAGTAGTGTGGAGGAGCTACTG	7	0.17500000000000002	No Hit
CCTCAAGTTCATAGTCTCCTGCAGACCCACGAGGGCACTGTGCCTTTGCT	7	0.17500000000000002	No Hit
CCACTTATGAGTGGCCTCTCACCGCTGTTGGAGAGACCTGACATTTACAA	7	0.17500000000000002	No Hit
GGACTGTCTTCCAAATAAGCTACCCATCTGCATTCAGCCAGCTGATCGCC	6	0.15	No Hit
CTCAGACTAAAGGTTTGCAAGCATAGATGGGACTATGGACTTTGAAAACA	6	0.15	No Hit
AAACCACCTTCCTTGAATGCTGGCTGTGAAATCATGAAAGAGTACATGGA	6	0.15	No Hit
AAGGACATGGTCATAAGAGAATTGTAGTGTTAAAAAATGACATGAAAAAC	6	0.15	No Hit
TCCATTTAAGTCAGCATTTGAATGACATATTGTGTATTGAGAGGTAATGG	6	0.15	No Hit
GTTCGCACCTCAAGTTCATAGTCTCCTGCAGACCCACGAGGGCACTGTGC	6	0.15	No Hit
AGCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCCCATTTTACCATG	6	0.15	No Hit
CTTCTTTTCTGAGGCCTGATAGCACTTCCTCTTTAAGGCCACATTGCACT	6	0.15	No Hit
AGGTATCGGAATGTAAGCGGAGAAGGAGCTGGTGGCTGAGGGTGGAAGAA	6	0.15	No Hit
GCAAGCATAGATGGGACTATGGACTTTGAAAACACTAGGTTTCACTAAAG	6	0.15	No Hit
AGACTATTGTGAATTGTGGTGGTTGGGAAGAAATAAGCAGAGATCGTTCA	6	0.15	No Hit
GAGCTTTCTAGAAAGTTATTTCATGCTTCCCTTCTCTGTCTGTCTCCGTT	6	0.15	No Hit
GTAACTGTTGCTCACATAAACGCTACCGCAAAGAATGCTGCTGATGATAA	6	0.15	No Hit
TGTGAATTGTGGTGGTTGGGAAGAAATAAGCAGAGATCGTTCAGCAGGAA	6	0.15	No Hit
CTGAAGCCTTGTTACATCATGCTAACCAGCTGATCAGATTTGAAGAGTTC	6	0.15	No Hit
GCAGTGATAAAGTTGCTGTTAGGAGTGTGAGTTGCCTCTTTTGCAGACGT	6	0.15	No Hit
AGTCAGCATTTGAATGACATATTGTGTATTGAGAGGTAATGGTTAGAGTC	6	0.15	No Hit
GTCTAAACAAGCCAGCAAGAAATAGTATAATTGATGCTGCTAAAATCTGG	6	0.15	No Hit
GAACTCCATTAAGGAATCTGTATTATATTTACCAAATTTTTCTAGACTAT	6	0.15	No Hit
GATTGAGTGGCAAGAGAGCCATTTTCCGGTTGACAGGAAAGTTCCTGGCT	6	0.15	No Hit
GGCCTCATCCCATTTTTTAAAAAAGTTTCCATTTGGGGATTCCATTTAAT	6	0.15	No Hit
AATTCTTTAAGGCTTCTAAGCAGTGATAAAGTTGCTGTTAGGAGTGTGAG	6	0.15	No Hit
CGCGCCTCCCTGGGATCTTCGCCGCCCGAGCTGGACATCATGCCACAGGA	6	0.15	No Hit
GGAAAGCCCAGCAAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGCCA	6	0.15	No Hit
CTGGGAAGCTCTCAATGTGAAGATGTGTGTGTCCATGTTAGTTCATCTTG	6	0.15	No Hit
AAGTTCCTGGCTTCTAGGTGACTTATTGGTGTGCCTTGTTTTTTATGTAA	6	0.15	No Hit
GCTAAAACTTTTGGTTATACATTTCGTCTCCAAGACTATGATGTCAGTTC	6	0.15	No Hit
GCAGGCTTGCCACTCTTTGTGAAATGCCTGAAGGTACTTTCTATGCAGGC	6	0.15	No Hit
GGATTCCATTTAATTAAGAGCTTTCTAGAAAGTTATTTCATGCTTCCCTT	6	0.15	No Hit
GGCAAGTCAGTCCTCTGTTCAGCTCTCAGTCTTGGTCTCCGAGGAGTATG	6	0.15	No Hit
ATGACATGGTCATTTGTATCCGAAAAAGAGAACGAACTCAGGATGAAATA	6	0.15	No Hit
CTTATGAGTGGCCTCTCACCGCTGTTGGAGAGACCTGACATTTACAATGG	6	0.15	No Hit
GGATTCACAACAAGCCCCCACCTCCCAACACTGACCCTTGGCTTCTTCGT	6	0.15	No Hit
ATTCCATTTAATTAAGAGCTTTCTAGAAAGTTATTTCATGCTTCCCTTCT	6	0.15	No Hit
CTAAAACTTTTGGTTATACATTTCGTCTCCAAGACTATGATGTCAGTTCA	6	0.15	No Hit
CTGCTCCAGCTACTGTGGTTCTTGTGTCAACTGATGTTAATTTTGCATTA	6	0.15	No Hit
AGGAGACCTCGTTGTTGGCAACCAGTCTAGGCAAAGCTGCTTCTAAATTT	6	0.15	No Hit
GCCCGAGCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCCCATTTTA	6	0.15	No Hit
CATCTATGAAAAGAAGTATGGACACAAGTTAAATGTGTCTGATTTATATA	6	0.15	No Hit
ATGGCCTTCTCTTCTGGGTGCCTGAGGTATCCACTTATGAGTGGCCTCTC	6	0.15	No Hit
CATGGTCATTTGTATCCGAAAAAGAGAACGAACTCAGGATGAAATAGAAA	6	0.15	No Hit
AGCGGTTCCGAAAGGGCTGGTCGGCAAGAAAGGCCTGCTAGCCCGCGCCT	6	0.15	No Hit
CTCTCACCGCTGTTGGAGAGACCTGACATTTACAATGGATTGTATCTGTT	6	0.15	No Hit
GAGAGGTAATGGTTAGAGTCTGATTTTTTAAACTATGCATTAAATGCATA	6	0.15	No Hit
AAAAATAGAGTCAAATTGGCAGCCAACTTTTCTTTTGCACCTGTGACCAA	6	0.15	No Hit
GGGTTTCAGACTATGGATATTCCAAGCTGATTGAGCTACTAGAAGCCGTG	6	0.15	No Hit
CTTGTTTGCAAAGAATGTGAGGTCTTTGCTTCATACCTATCACTACCAGC	6	0.15	No Hit
CTTTTTCTTACTTTATTTCTTCCAGTTATTTGAAAGAACTCCATTAAGGA	6	0.15	No Hit
GTTGCTGTTAGGAGTGTGAGTTGCCTCTTTTGCAGACGTCCTAAGGCTAA	6	0.15	No Hit
CTGATCGCCTCAAGGCAAGTCAGTCCTCTGTTCAGCTCTCAGTCTTGGTC	6	0.15	No Hit
GGACATCATGCCACAGGAAGAGTGAGCTCTTGCCCATTTTACCATGGAGG	6	0.15	No Hit
TGCTCCTCCCAAACCCTCCTCAGAAGCCCCCGGGCTACTGATTAGCAAGG	5	0.125	No Hit
AGTTATTTGAAAGAACTCCATTAAGGAATCTGTATTATATTTACCAAATT	5	0.125	No Hit
GGTACTTTCTATGCAGGCCTGGCTGCACTTCTTTTCTGAGGCCTGATAGC	5	0.125	No Hit
CTTTAAGGCCACATTGCACTTCCTCTGCAATCTTAGTGCACATGTGCAAT	5	0.125	No Hit
AGCTGCTTCTAAATTTGGGTCACCAGAAGTTGCAGTCACTGGACAGGTGC	5	0.125	No Hit
GTTCAGCTCTCAGTCTTGGTCTCCGAGGAGTATGTCTCCAAACCTTTTGA	5	0.125	No Hit
AAGAAGGGGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGC	5	0.125	No Hit
TGCCTAACAGTGGCCCAACCGCTTCAGTGCCCACACTGAAAAACACAGGT	5	0.125	No Hit
CAACCAGTCTAGGCAAAGCTGCTTCTAAATTTGGGTCACCAGAAGTTGCA	5	0.125	No Hit
GTTGGAGTATCACGAATCTGTGTGCAAGCAGCACTGCTCGAACAAGGACT	5	0.125	No Hit
CCCGAGCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCCCATTTTAC	5	0.125	No Hit
GTCAAATTGGCAGCCAACTTTTCTTTTGCACCTGTGACCAAGCTCTAACT	5	0.125	No Hit
AGAAACACTGCAGCCCAAAACTTATGGCTACAGTAGTGTGGAGGAGCTAC	5	0.125	No Hit
TGATAGCACTTCCTCTTTAAGGCCACATTGCACTTCCTCTGCAATCTTAG	5	0.125	No Hit
GTCACACACTGGGGCATGATTGAGTGGCAAGAGAGCCATTTTCCGGTTGA	5	0.125	No Hit
TCTCTTCTGGGTGCCTGAGGTATCCACTTATGAGTGGCCTCTCACCGCTG	5	0.125	No Hit
GCTAAGTTCATTTGACCTTTCCCCTGTCAATCATGAAGACCAGCCTGCAG	5	0.125	No Hit
CATCACCATTTTGGAAAGCAGTGCCGGGTTTCAGACTATGGATATTCCAA	5	0.125	No Hit
CAATTCTTTAAGGCTTCTAAGCAGTGATAAAGTTGCTGTTAGGAGTGTGA	5	0.125	No Hit
GCTTGCCTGCTCAGACTAAAGGTTTGCAAGCATAGATGGGACTATGGACT	5	0.125	No Hit
ATGGGACTATGGACTTTGAAAACACTAGGTTTCACTAAAGTTACTTGGAG	5	0.125	No Hit
CCTGCTCAGACTAAAGGTTTGCAAGCATAGATGGGACTATGGACTTTGAA	5	0.125	No Hit
CTATTCTTCAGGATGCTCCTGCCTGTTGTTTGCCTCTCTTTAAATTTATA	5	0.125	No Hit
AGTGCACATGTGCAATTCTTTAAGGCTTCTAAGCAGTGATAAAGTTGCTG	5	0.125	No Hit
GTGCAAACTTGCATATTATGGATTTACCAAGTTGCTGGAACTTTTGGAAG	5	0.125	No Hit
GATGACATGGTCATTTGTATCCGAAAAAGAGAACGAACTCAGGATGAAAT	5	0.125	No Hit
ATTTAATTAAGAGCTTTCTAGAAAGTTATTTCATGCTTCCCTTCTCTGTC	5	0.125	No Hit
GCAAAGAATGCTGCTGATGATAAACTCCGTCAGAGCCTGCGAAGGTTTGC	5	0.125	No Hit
CTCACAGAGATTGGTGAGAAGGGTGTGAACCTGTCGGGGGGCCAGAAGCA	5	0.125	No Hit
CAACACTGACCCTTGGCTTCTTCGTTCTAAAAGCCCTGTGAGAAATCCTC	5	0.125	No Hit
GAGCCTGCGAAGGTTTGCAAATACGCACACTGCCCAGCTACTGTGGTTCT	5	0.125	No Hit
ACTCCTCACCCAGTAGCCCAGCATCTGCTTCTGCTCCTCCCAAACCCTCC	5	0.125	No Hit
GAGAATTGTAGTGTTAAAAAATGACATGAAAAACCGGCTAAGTTCATTTG	5	0.125	No Hit
GTTTATATTATTTTTTCTAGAAAGCCAGAAAATGGATTCTTATTTCATGG	5	0.125	No Hit
ACCGCTTCAGTGCCCACACTGAAAAACACAGGTGTGAGGGAGACACTTCA	5	0.125	No Hit
TGTGGTTCTTGTGTCAACTGATGTTAATTTTGCATTAGAACTCAGTGATC	5	0.125	No Hit
TGAAACTTCTCAAGTCCCAGGCCAGCAAGCAGGTCATTGTGAGCGATTTC	5	0.125	No Hit
AGCTACAGCAGCTCCTCCAAGAAGCCTTCTCCAAGCATGGCCAGGTGAAG	5	0.125	No Hit
ATTTGAAAGAACTCCATTAAGGAATCTGTATTATATTTACCAAATTTTTC	5	0.125	No Hit
CCTTGCCACACTCTACTCTATGTTTATAACCTGCCGGCAAATAAAGATGG	5	0.125	No Hit
GTTTTATTTTTAAACCAAGAAGAAAAAACAGAAATTCCAATACCAGTAAA	5	0.125	No Hit
CTGACCCTTGGCTTCTTCGTTCTAAAAGCCCTGTGAGAAATCCTCAGCTG	5	0.125	No Hit
GCGAAGGTTTGCAAATACGCACACTGCTCCAGCTACTGTGGTTCTTGTGT	5	0.125	No Hit
TGTCCATGTTAGTTCATCTTGACGTCTTTAATAGTGTCTAGCTTTACTGT	5	0.125	No Hit
CCTCCATCATTTGTTTAAGCAAATGTATTTGTGGTTGTTCAAGAAGCCAA	5	0.125	No Hit
TTTGTATCCGAAAAAGAGAACGAACTCAGGATGAAATAGAAAGGACAAAG	5	0.125	No Hit
AGGAAGTGGATTGAGTCCCATTGAGCAGCCTCCATCATTTGTTTAAGCAA	5	0.125	No Hit
CTGGGATCTTCGCCGCCCGAGCTGGACATCATGCCACAGGAAGAGTGAGC	5	0.125	No Hit
CAAAAATAGAGTCAAATTGGCAGCCAACTTTTCTTTTGCACCTGTGACCA	5	0.125	No Hit
CTAGAAGCCGTGCCTCATGTGTTGCAGATTCTTGGAATGGGCTCCAAGCG	5	0.125	No Hit
TGATTGAGTGGCAAGAGAGCCATTTTCCGGTTGACAGGAAAGTTCCTGGC	5	0.125	No Hit
ACTTTTTCTTACTTTATTTCTTCCAGTTATTTGAAAGAACTCCATTAAGG	5	0.125	No Hit
GAACAAGGAAGTCATTCAAGAGCTGAATAATTGTCAGGTAACTGTTGCTC	5	0.125	No Hit
CGGCAAGAAAGGCCTGCTAGCCCGCGCCTCCCTGGGATCTTCGCCGCCCG	5	0.125	No Hit
GACATTGCCTCTTAGTTCCCAAACGAAAGAGTACATGGAGAACAAGAAAG	5	0.125	No Hit
CCCAGTTCTCAGCACTATGACCTGCCCCAGATGGAGCTGCTCTAGAAATG	5	0.125	No Hit
GGTTACCACTGAAGATTCCTTGCCACACTCTACTCTATGTTTATAACCTG	5	0.125	No Hit
AGCTAAATGTGTCTGATTTATATAAATTAACAGACATGATTGCAATCCGT	5	0.125	No Hit
TCTATTCTTCAGGATGCTCCTGCCTGTTGTTTGCCTCTCTTTAAATTTAT	5	0.125	No Hit
CTGTAACTCCTCACCCAGTAGCCCAGCATCTGCTTCTGCTCCTCCCAAAC	5	0.125	No Hit
GGTTAACCAATGTGGCCTCATCCCATTTTTTAAAAAAGTTTCCATTTGGG	5	0.125	No Hit
TAAGCAGTGATAAAGTTGCTGTTAGGAGTGTGAGTTGCCTCTTTTGCAGA	5	0.125	No Hit
ATTCATAGAGCAGAGAGAACCTGTTGGCACACAGTCTTCCACTAGTCAGA	5	0.125	No Hit
AGGGAAATGTTTGTTTCCAACTTTCAGATGAACTTTTTCTTACTTTATTT	5	0.125	No Hit
GACACTGCCTTGTGGAATGGCCTTCTCTTCTGGGGAAGATGTGTGTGTCC	5	0.125	No Hit
GCCTTCTTTAGTAGAGGTTTTTATGAATGATAAGGCAGAAGAGTGTGTGA	5	0.125	No Hit
CAGAGAAGCAGAGTTTATCTGTGTGTGTGACATCAGTAAAGAGAACAAGG	5	0.125	No Hit
CAAGCATAGATGGGACTATGGACTTTGAAAACACTAGGTTTCACTAAAGT	5	0.125	No Hit
GGCCAGGTGAAGAGTGTTGAGCTCAGTCCCCATACTGATTACCAGCTCAA	5	0.125	No Hit
GTGAGTTGCCTCTTTTGTAGACGTCCTAAGGCTAAGCTTGCCTGCTCAGA	5	0.125	No Hit
GTCATAAGAGAATTGTAGTGTTAAAAAATGACATGAAAAACCGGCTAAGT	5	0.125	No Hit
TTCTGCTCCTCCCAAACCCTCCTCAGAAGCCCCCGGGCTACTGATTAGCA	5	0.125	No Hit
CTGTAATTTGAAGGCCAAGGTTGATCCTTGTTCTTGTGATTTAAAAATTG	5	0.125	No Hit
CAGCACTGCTCGAACAAGGACTTCAGCGAACTTGTATTTGATCCAGACTC	5	0.125	No Hit
AGATGGGACTATGGACTTTGAAAACACTAGGTTTCACTAAAGTTACTTGG	5	0.125	No Hit
TGCAGACCCACGAGGGCACTGTGCCTTTGCTGAGCTTTCCAGATTGCTAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0	0.0	0.0	0.0
138-139	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATCACC	20	0.0034199494	178.62068	145
>>END_MODULE
SRR26281820 read2 length is 46-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR26281820_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	46-151
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.1915	37.0	37.0	37.0	37.0	37.0
2	36.2855	37.0	37.0	37.0	37.0	37.0
3	36.3685	37.0	37.0	37.0	37.0	37.0
4	36.323	37.0	37.0	37.0	37.0	37.0
5	36.303	37.0	37.0	37.0	37.0	37.0
6	36.4165	37.0	37.0	37.0	37.0	37.0
7	36.113	37.0	37.0	37.0	37.0	37.0
8	36.336	37.0	37.0	37.0	37.0	37.0
9	36.3435	37.0	37.0	37.0	37.0	37.0
10-14	36.3069	37.0	37.0	37.0	37.0	37.0
15-19	36.3176	37.0	37.0	37.0	37.0	37.0
20-24	36.3476	37.0	37.0	37.0	37.0	37.0
25-29	36.3045	37.0	37.0	37.0	37.0	37.0
30-34	36.2625	37.0	37.0	37.0	37.0	37.0
35-39	36.19279999999999	37.0	37.0	37.0	37.0	37.0
40-44	36.1991	37.0	37.0	37.0	37.0	37.0
45-49	36.169677119279825	37.0	37.0	37.0	37.0	37.0
50-54	36.20050037528146	37.0	37.0	37.0	37.0	37.0
55-59	36.13870806209314	37.0	37.0	37.0	37.0	37.0
60-64	36.08804155394123	37.0	37.0	37.0	37.0	37.0
65-69	36.13357508320793	37.0	37.0	37.0	37.0	37.0
70-74	36.065275105110416	37.0	37.0	37.0	37.0	37.0
75-79	36.08170149872631	37.0	37.0	37.0	37.0	37.0
80-84	36.03016407956022	37.0	37.0	37.0	37.0	37.0
85-89	36.007523746483216	37.0	37.0	37.0	37.0	37.0
90-94	35.9943899531249	37.0	37.0	37.0	37.0	37.0
95-99	35.896503731082795	37.0	37.0	37.0	37.0	37.0
100-104	35.903444663757064	37.0	37.0	37.0	37.0	37.0
105-109	35.92879158657572	37.0	37.0	37.0	37.0	37.0
110-114	35.875208849297834	37.0	37.0	37.0	37.0	37.0
115-119	35.90603856895713	37.0	37.0	37.0	37.0	37.0
120-124	35.860875002741025	37.0	37.0	37.0	37.0	37.0
125-129	35.77105209891566	37.0	37.0	37.0	37.0	37.0
130-134	35.61774516079437	37.0	37.0	37.0	37.0	37.0
135-139	35.42587209941204	37.0	37.0	37.0	37.0	37.0
140-144	35.49861272474722	37.0	37.0	37.0	37.0	37.0
145-149	35.315948419249274	37.0	37.0	37.0	32.2	37.0
150-151	35.59265314257967	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	2.0
25	2.0
26	8.0
27	18.0
28	21.0
29	17.0
30	37.0
31	51.0
32	70.0
33	115.0
34	169.0
35	379.0
36	2646.0
37	465.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.900000000000002	9.9	16.825000000000003	41.375
2	24.325	8.450000000000001	30.975	36.25
3	20.974999999999998	12.55	22.825	43.65
4	23.799999999999997	16.85	19.45	39.900000000000006
5	24.4	24.925	24.375	26.3
6	28.599999999999998	25.5	22.875	23.025000000000002
7	19.275000000000002	30.075000000000003	33.900000000000006	16.75
8	20.974999999999998	27.474999999999998	31.825	19.725
9	22.125	24.55	30.725	22.6
10-14	22.95	30.330000000000002	27.255000000000003	19.465
15-19	20.9	28.82	27.389999999999997	22.89
20-24	21.154999999999998	25.85	31.455	21.54
25-29	22.215	27.439999999999998	28.360000000000003	21.985
30-34	20.69	28.84	27.485	22.985
35-39	21.455	26.555	30.130000000000003	21.86
40-44	21.625	27.694999999999997	30.3	20.380000000000003
45-49	22.32834925238786	27.17907686152923	26.739010851627743	23.75356303445517
50-54	21.92644483362522	29.02176632474356	25.068801601200903	23.982987240430322
55-59	21.066599899849773	26.204306459689537	28.427641462193293	24.301452178267404
60-64	20.983573717948715	28.240184294871796	27.779447115384613	22.996794871794872
65-69	22.66279011479272	27.57531705849917	28.407438969371903	21.35445385733621
70-74	22.181434174796337	27.999597706929496	26.003218344564015	23.81574977371015
75-79	21.74396281701526	27.614428614731736	27.695261190259675	22.94634737799333
80-84	19.910446242303976	27.883783646262657	29.110059532895743	23.095710578537627
85-89	21.665041318072163	28.896987116973772	27.93204331981728	21.505928245136786
90-94	20.80223880597015	30.032131011608627	27.005597014925375	22.160033167495854
95-99	23.68296654236042	28.914333736015546	26.36167865959347	21.04102106203057
100-104	22.308146716073253	26.083454164652814	31.319478008699853	20.288921110574083
105-109	22.709755560449242	28.628055494384498	28.07751596564633	20.58467297951993
110-114	22.56439286725163	26.096801585055196	27.59694310784036	23.741862439852817
115-119	22.9887095820772	25.341372583791454	27.2979842761719	24.371933557959448
120-124	19.996253044401424	27.7836757634422	27.26534690563917	24.954724286517205
125-129	23.740488060876412	23.609288900551036	29.316452374704806	23.33377066386775
130-134	23.016822693038645	25.67748293094953	29.626240585626807	21.679453790385022
135-139	18.603435399551906	27.856609410007465	28.946975354742342	24.59297983569828
140-144	21.178306671868903	28.15450643776824	28.997268825595008	21.669918064767852
145-149	20.758984669514955	28.080757309206668	27.159252743570413	24.001005277707968
150-151	21.90142898804316	13.99825021872266	36.77456984543599	27.325750947798195
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	2.5
27	4.5
28	5.0
29	9.5
30	17.0
31	27.5
32	46.0
33	52.0
34	68.0
35	104.5
36	108.5
37	103.0
38	106.0
39	125.0
40	154.5
41	169.5
42	196.5
43	199.5
44	224.5
45	330.0
46	334.5
47	220.0
48	151.0
49	157.5
50	178.5
51	192.0
52	176.5
53	132.5
54	90.0
55	56.0
56	77.5
57	98.5
58	69.0
59	35.5
60	16.5
61	9.0
62	4.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
45-49	3.0
50-54	3.0
55-59	0.0
60-64	1.0
65-69	12.0
70-74	12.0
75-79	24.0
80-84	36.0
85-89	35.0
90-94	33.0
95-99	69.0
100-104	102.0
105-109	91.0
110-114	133.0
115-119	154.0
120-124	177.0
125-129	181.0
130-134	214.0
135-139	108.0
140-144	130.0
145-149	436.0
150-152	2046.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	46.61508704061896	18.075
2	19.987105093488072	15.5
3	14.829142488716956	17.25
4	7.221147646679562	11.200000000000001
5	3.67504835589942	7.124999999999999
6	2.4500322372662797	5.7
7	1.5473887814313347	4.2
8	0.6447453255963894	2.0
9	0.5802707930367506	2.025
>10	2.385557704706641	15.375
>50	0.06447453255963895	1.55
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCCCAGATCACACACCCATTCACTGTACACAACTCTTCTTTGTTGCCCAC	62	1.55	No Hit
CCTCGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTTCT	49	1.225	No Hit
CTCCCCAGATCACACACCCATTCACTGTACACAACTCTTCTTTGTTGCCC	45	1.125	No Hit
CTCGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTTCTC	37	0.9249999999999999	No Hit
CCCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGTC	35	0.8750000000000001	No Hit
GTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACCTCC	35	0.8750000000000001	No Hit
GTTGAGAGGAGTACTGTGAGTGGTTTCATAATGTCTCTTGAGCTCATCAA	19	0.475	No Hit
TCCCCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGG	18	0.44999999999999996	No Hit
CCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGTCA	18	0.44999999999999996	No Hit
CCTCTCAATACACAATATGTCATTCAAATGCTGACTTAAATGGATGACAT	18	0.44999999999999996	No Hit
CCCCAGTGTGTGACCCACAGGTTTAAGGACATGTTTGGCTTCTTGAACAA	18	0.44999999999999996	No Hit
GCCGGTTTTTCATGTCATTTTTTAACACTACAATTCTCTTATGACCATGT	17	0.42500000000000004	No Hit
TCTCTCCAATAGTTTGTTTCTTCCTCCTCTGATCCAAAAGTCTTTCCTGA	15	0.375	No Hit
CTCTCAATACACAATATGTCATTCAAATGCTGACTTAAATGGATGACATC	14	0.35000000000000003	No Hit
CTCTCCAATAGTTTGTTTCTTCCTCCTCTGATCCAAAAGTCTTTCCTGAG	14	0.35000000000000003	No Hit
CCCAGTGTGTGACCCACAGGTTTAAGGACATGTTTGGCTTCTTGAACAAC	13	0.325	No Hit
CCCAGATCACACACCCATTCACTGTACACAACTCTTCTTTGTTGCCCACA	13	0.325	No Hit
GTTGGGAGGTGGGGGCTTGTTGTGAATCCACTTCACTACTTTGACGCCAT	13	0.325	No Hit
GTAAGAGGGAATGAACTTGTTAAAGGGCATTCGGAAATGGGGCTGGTGGC	13	0.325	No Hit
GTCCGCAAAGGCTAGCTTTTCCCAAATGCCAACATCAGCTACCATAATAA	13	0.325	No Hit
CTTCCCAGATTTACAGCCAATTTTTAAATCACAAGAACAAGGATCAACCT	13	0.325	No Hit
CTCCAATAGTTTGTTTCTTCCTCCTCTGATCCAAAAGTCTTTCCTGAGTC	12	0.3	No Hit
TCCCTCGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTT	12	0.3	No Hit
TCCCCAGATCACACACCCATTCACTGTACACAACTCTTCTTTGTTGCCCA	12	0.3	No Hit
GGCCAGGTCTCTCCAATAGTTTGTTTCTTCCTCCTCTGATCCAAAAGTCT	12	0.3	No Hit
CCCCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGT	12	0.3	No Hit
CTCTCTTGCCACTCAATCATGCCCCAGTGTGTGACCCACAGGTTTAAGGA	12	0.3	No Hit
CCCTTATCAAACGGAGACAGACAGAGAAGGGAAGCATGAAATAACTTTCT	12	0.3	No Hit
GTTGGTTCTTTAGTAAATCAATGACTTCTCTGCTGAACTGAATCAGCTGA	11	0.27499999999999997	No Hit
CTGGCCATGAAATAAGAATCCATTTTCTGGCTTTCTAGAAAAAATAATAT	10	0.25	No Hit
GGCAGGTCATAGTGCTGAGAACTGGGAATCACTGGCTTCATTTAAAAGAT	10	0.25	No Hit
CGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTTCTCTC	10	0.25	No Hit
GCTCTTAATTAAATGGAATCCCCAAATGGAAACTTTTTTAAAAAATGGGA	10	0.25	No Hit
CTCCAGGATCTGCCTGCTGACTGCAGGCTGGTCTTCATGATTGACAGGGG	10	0.25	No Hit
CCACTCAGCTGATTTACAATTTATGCATTTAATGCATAGTTTAAAAAATC	10	0.25	No Hit
GGGGCAGGTCATAGTGCTGAGAACTGGGAATCACTGGCTTCATTTAAAAG	10	0.25	No Hit
GCCGGCAGGTTATAAACATAGAGTAGAGTGTGGCAAGGAATCTTCAGTGG	10	0.25	No Hit
TGCCAATTTGACTCTATTTTTGGGCTGCTTTTTTGCTGGGCTTTCCACTG	10	0.25	No Hit
CTCTGATCCAAAAGTCTTTCCTGAGTCCTCCATGGTAAAATGGGCAAGAG	9	0.22499999999999998	No Hit
CGGCAGGTTATAAACATAGAGTAGAGTGTGGCAAGGAATCTTCAGTGGTA	9	0.22499999999999998	No Hit
GGGCTGGTGGCGCAGCAAGTCCACAACATCTTTGGAGAACTGCTTTGTCC	9	0.22499999999999998	No Hit
CTGCCGGCCAAAGTGGTGGTGGTAAGAGGGAATGAACTTGTTAAAGGGCA	9	0.22499999999999998	No Hit
CTGGGGCAGGTCATAGTGCTGAGAACTGGGAATCACTGGCTTCATTTAAA	9	0.22499999999999998	No Hit
GCCGGCCAAAGTGGTGGTGGTAAGAGGGAATGAACTTGTTAAAGGGCATT	9	0.22499999999999998	No Hit
GGCCTGGTTACTGGGTAAGAGACACACTAGTCGCCCATTTCCCTGTTCAC	9	0.22499999999999998	No Hit
ATGAAATAAGAATCCATTTTCTGGCTTTCTAGAAAAAATAATATAAACAT	9	0.22499999999999998	No Hit
TTCCCAGATTTACAGCCAATTTTTAAATCACAAGAACAAGGATCAACCTT	9	0.22499999999999998	No Hit
ATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGTCACAGGTGCAAA	8	0.2	No Hit
CCCACACCATTTGAAGCACTTCACCACTCAGCTGATTTACAATTTATGCA	8	0.2	No Hit
GCCAGATTTTAGCAGCATCAATTATACTATTTCTTGCTGGCTTGTTTAGA	8	0.2	No Hit
GTGGAAACCATGCCGGTGCCTGAGATCACTGAGTTCTAATGCAAAATTAA	8	0.2	No Hit
AGCTTATTTGGAAGACAGTCCCCTCTCTCTTTTTCTTCTGTGCAGGAGAG	8	0.2	No Hit
CACCACTCAGCTGATTTACAATTTATGCATTTAATGCATAGTTTAAAAAA	8	0.2	No Hit
GCGGTGTTCAAAAGGTTTGGAGACATACTCCTCGGAGACCAAGACTGAGA	8	0.2	No Hit
CTCTTGAGCTCATCAACGGGAAGATAAGCATCTCCTTCCCAAGACATCAA	8	0.2	No Hit
GTGGCAATGTTCACTCCTGGAATGCAGGTGATGAAGTGCTCTAAGGGGAC	8	0.2	No Hit
GCCACTCAGCTGATTTACAATTTATGCATTTAATGCATAGTTTAAAAAAT	8	0.2	No Hit
CCCAAACATGCTTCCAACAGTGAACAGTAACAGTAAAGCTAGACACTATT	7	0.17500000000000002	No Hit
CTCTGGATAAAGAAATAGTGATTGGACTGGCCTCTTGGGCATGTCACGCT	7	0.17500000000000002	No Hit
CCCAAATGGAAACTTTTTTAAAAAATGGGATGAGGCCACATTGGTTAACC	7	0.17500000000000002	No Hit
AAAATGACATGAAAAACCGGCTAAGTTCATTTGACCTTTCCCCTGTCAAT	7	0.17500000000000002	No Hit
CGGACCATTAATAAAAGTGGCTTCTGGGGTCAAGACATGAAATAAGGTTT	7	0.17500000000000002	No Hit
GCGCTACTGGGATTGGCAATGTTGAAAGCAAGTGGCGAGGCGGTGTTCAA	7	0.17500000000000002	No Hit
GCCTGAGATCACTGAGTTCTAATGCAAAATTAACATCAGTTGACACAAGA	7	0.17500000000000002	No Hit
ACCGGCCAGAGGGGACTGAGCAGTTTTCAATATCCCAGAAAACACCAATG	7	0.17500000000000002	No Hit
GGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAGAATCA	7	0.17500000000000002	No Hit
CTACTGGGATTGGCAATGTTGAAAGCAAGTGGCGAGGCGGTGTTCAAAAG	7	0.17500000000000002	No Hit
CCCAGATTTACAGCCAATTTTTAAATCACAAGAACAAGGATCAACCTTGG	7	0.17500000000000002	No Hit
CTGCAAAAGAGGCAACTCACACTCCTAACAGCAACTTTATCACTGCTTAG	7	0.17500000000000002	No Hit
TGGGCCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACC	7	0.17500000000000002	No Hit
GGGCTGCTTTTTTGCTGGGCTTTCCACTGCATCCTTGCTAATCAGTAGCC	7	0.17500000000000002	No Hit
ATGCAAGATGGTTGGTTCTTTAGTAAATCAATGACTTCTCTGCTGAACTG	7	0.17500000000000002	No Hit
GGGCATTCGGAAATGGGGCTGGTGGCGCAGCAAGTCCACAACATCTTTGG	7	0.17500000000000002	No Hit
GGGTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACCT	7	0.17500000000000002	No Hit
GCTGCTTTTTTGCTGGGCTTTCCACTGCATCCTTGCTAATCAGTAGCCCG	7	0.17500000000000002	No Hit
GGTCAGTGTTGGGAGGTGGGGGCTTGTTGTGAATCCACTTCACTACTTTG	7	0.17500000000000002	No Hit
GGACTGAGCAGTTTTCAATATCCCAGAAAACACCAATGGGGGGGAGGGTC	7	0.17500000000000002	No Hit
TTCCGGGTTATCAGTTGGATTTGTTTGCCAGACTCCATATCAGCAACCTT	7	0.17500000000000002	No Hit
GCTGAGTACTTGGAGGTGGTATATTTGGCCAGATTTTAGCAGCATCAATT	7	0.17500000000000002	No Hit
GCTTTTTTGCTGGGCTTTCCACTGCATCCTTGCTAATCAGTAGCCCGGGG	7	0.17500000000000002	No Hit
TTGGTCACAGGTGCAAAAGAAAAGTTGGCTGCCAATTTGACTCTATTTTT	7	0.17500000000000002	No Hit
ATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAGAATCAGACAGTTTT	6	0.15	No Hit
CTGCTCAATGGGACTCAATCCACTTCCTGCTGAACGATCTCTGCTTATTT	6	0.15	No Hit
GGTCAGTAGACGCTTGGAGCCCATTCCAAGAATCTGCAACACATGAGGCA	6	0.15	No Hit
GCTGGGTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCA	6	0.15	No Hit
ATTGGTAGTATGCAAGATGGTTGGTTCTTTAGTAAATCAATGACTTCTCT	6	0.15	No Hit
CTTGGTCACAGGTGCAAAAGAAAAGTTGGCTGCCAATTTGACTCTATTTT	6	0.15	No Hit
GGTTCTTTAGTAAATCAATGACTTCTCTGCTGAACTGAATCAGCTGAGGA	6	0.15	No Hit
GTAATTTCTAGGTCACCAAACTCCGCAGCATAGCAATCTGGAAAGCTCAG	6	0.15	No Hit
GCCAGAGGGGACTGAGCAGTTTTCAATATCCCAGAAAACACCAATGGGGG	6	0.15	No Hit
CCTCTGATCCAAAAGTCTTTCCTGAGTCCTCCATGGTAAAATGGGCAAGA	6	0.15	No Hit
CCCCCAGATCACACACCCATTCACTGTACACAACTCTTCTTTGTTGCCCA	6	0.15	No Hit
ACTGTCATCATAAGGCAGTTACCCTTTTGGGAACGCAAAAGCTTAACAAA	6	0.15	No Hit
CGGCTGCTCAATGGGACTCAATCCACTTCCTGCTGAACGATCTCTGCTTA	6	0.15	No Hit
CTGGCCATGCTTGGAGAAGGCTTCTTGGAGGAGCTGCTGTAGCTCCTTCC	6	0.15	No Hit
CCTGAGATCACTGAGTTCTAATGCAAAATTAACATCAGTTGACACAAGAA	6	0.15	No Hit
TATCAGTTGGATTTGTTTGCCAGACTCCATATCAGCAACCTTTACAACAT	6	0.15	No Hit
TGCTGGTAGTGATAGGTATGAAGCAAAGACCTCACATTCTTTGCAAACAA	6	0.15	No Hit
GCCATCTTTATTTGCCGGCAGGTTATAAACATAGAGTAGAGTGTGGCAAG	6	0.15	No Hit
CTTCATTTTCCATTCGCTTCTGGGCCCGCTCTGCGCTGTCTTGGTTTATA	6	0.15	No Hit
TTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACCTCC	6	0.15	No Hit
GGAGGCTGTTCACAGCACAGATTGCGTCATGCAGGTTTCTCATCTGCACG	6	0.15	No Hit
TTGCCCACAGAACCTGACCTTTGAGATGACTCCAAGTAACTTTAGTGAAA	6	0.15	No Hit
GGTCACAGGTGCAAAAGAAAAGTTGGCTGCCAATTTGACTCTATTTTTGG	6	0.15	No Hit
GCACATGTGCACTAAGATTGCAGAGGAAGTGCAATGTGGCCTTAAAGAGG	6	0.15	No Hit
TCATTGGGGCAATGAGCACAGCAGCTTACTTTGGGAATAGCTGAGAGCTG	6	0.15	No Hit
TCCGCAAAGGCTAGCTTTTCCCAAATGCCAACATCAGCTACCATAATAAT	6	0.15	No Hit
CTGGGGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAGA	6	0.15	No Hit
GTGGCTTCTGGGGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGT	6	0.15	No Hit
CCACGGCTTAGCATCGTTATTTTGGTGTAACCATCCAAGTGTTCTGCTGC	6	0.15	No Hit
CTTCACTACTTTGACGCCATTCTGAGCAGTGGCAATGTTCACTCCTGGAA	6	0.15	No Hit
CCCATTCACTGTACACAACTCTTCTTTGTTGCCCACAGAACCTGACCTTT	6	0.15	No Hit
GGAGGCTGCTCAATGGGACTCAATCCACTTCCTGCTGAACGATCTCTGCT	6	0.15	No Hit
CCGGCAGGTTATAAACATAGAGTAGAGTGTGGCAAGGAATCTTCAGTGGT	6	0.15	No Hit
GGGAGACTTTGCTTTATCAACAACAGCATTCGAACTCTTGGCTTCAAAGC	6	0.15	No Hit
CCCTCGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTTC	6	0.15	No Hit
TCAGCTTCATATTCCCGGCTTCCTTCCTGGAATTCTCGGAGTTCCTCCTG	6	0.15	No Hit
GCCTGGGACTTGAGAAGTTTCAGTAAATCCTGAGTAAAGCGCTTCACCTG	6	0.15	No Hit
CGGCCAAAGTGGTGGTGGTAAGAGGGAATGAACTTGTTAAAGGGCATTCG	6	0.15	No Hit
CCGGCCAGAGGGGACTGAGCAGTTTTCAATATCCCAGAAAACACCAATGG	5	0.125	No Hit
CTCTTAATTAAATGGAATCCCCAAATGGAAACTTTTTTAAAAAATGGGAT	5	0.125	No Hit
GCAATGTTGAAAGCAAGTGGCGAGGCGGTGTTCAAAAGGTTTGGAGACAT	5	0.125	No Hit
GCCACATTGGTTAACCACAGACATAGTTACATAAAAAACAAGGCACACCA	5	0.125	No Hit
GTTGGCAATGTTCACTCCTGGAATGCAGGTGATGAAGTGCTCTAAGGGGA	5	0.125	No Hit
GTGGCCTTAAAGAGGAAGTGCTATCAGGCCTCAGAAAAGAAGTGCAGCCA	5	0.125	No Hit
GCCGCAAAGACTTCCGGGTTATCAGTTGGATTTGTTTGCCAGACTCCATA	5	0.125	No Hit
CCTCTGGATAAAGAAATAGTGATTGGACTGGCCTCTTGGGCATGTCACGC	5	0.125	No Hit
ACTGGGGCAGGTCATAGTGCTGAGAACTGGGAATCACTGGCTTCATTTAA	5	0.125	No Hit
GGGGGTTCTGGCACCTCCAGGATCTGCCTGCTGACTGCAGGCTGGTCTTC	5	0.125	No Hit
CCCGGTTTCTGGTTTCAGCTTGCTGCAGCTGAGTCTCCAATTCAGCTTCA	5	0.125	No Hit
CCTGTACTCATTGTCTATCCAATGTGCCATGTCCTGCACATAGTTACCGT	5	0.125	No Hit
CCATCTCTTTTCCTTAAACCTTACCCTTATCAAACGGAGACAGACAGAGA	5	0.125	No Hit
CGACAGGGGAAAGGTCAAATGAACTTAGCCGGTTTTTCATGTCATTTTTT	5	0.125	No Hit
GCTCTCTTGCCACTCAATCATGCCCCAGTGTGTGACCCACAGGTTTAAGG	5	0.125	No Hit
TCCAGCTCCATTCGAAGGCGGTTATTCTCTGATAATAGGTCCCGGTTTCT	5	0.125	No Hit
TACCCAAACATGCTTCCAACAGTGAACAGTAACAGTAAAGCTAGACACTA	5	0.125	No Hit
GGGAGGTGGGGGCTTGTTGTGAATCCACTTCACTACTTTGACGCCATTCT	5	0.125	No Hit
TCCACGGCTTAGCATCGTTATTTTGGTGTAACCATCCAAGTGTTCTGCTG	5	0.125	No Hit
TGTGGTAAGAGGGAATGAACTTGTTAAAGGGCATTCGGAAATGGGGCTGG	5	0.125	No Hit
GCCAACAGGTTCTCTCTGCTCTATGAATGGATTTGCCCTTTTTGCCCAAC	5	0.125	No Hit
CACCAAACTCCGCAGCATAGCAATCTGGAAAGCTCAGCAAAGGCACAGTG	5	0.125	No Hit
CTCACGGATTCTCTGTACAACAGTTGTTGCTGACCGGCCAGAGGGGACTG	5	0.125	No Hit
CCAGAAAACACCAATGGGGGGGAGGGTCTCCAGCACCTGTCCAGTGACTG	5	0.125	No Hit
GCCTGTGATACTTAGCACCTTGCCACCACAGTTATCAGAGAGGCGCCTGA	5	0.125	No Hit
CAGCAATGGTTAGTCACTCCCCAGATCACACACCCATTCACTGTACACAA	5	0.125	No Hit
CCGGGGCAGGTCATAGTGCTGAGAACTGGGAATCACTGGCTTCATTTAAA	5	0.125	No Hit
CGGTTTTTCATGTCATTTTTTAACACTACAATTCTCTTATGACCATGTCC	5	0.125	No Hit
GCCCCAGTGTGTGACCCACAGGTTTAAGGACATGTTTGGCTTCTTGAACA	5	0.125	No Hit
CGGATTGCAATCATGTCTGTTAATTTATATAAATCAGACACATTTAACTT	5	0.125	No Hit
CTCTATGAATGGATTTGCCCTTTTTGCCCAACAGATACAATCCATTGTAA	5	0.125	No Hit
ACTCCCCAGATCACACACCCATTCACTGTACACAACTCTTCTTTGTTGCC	5	0.125	No Hit
ACCTGCTTGCTGGCCTGGGACTTGAGAAGTTTCAGTAAATCCTGAGTAAA	5	0.125	No Hit
CAGGCACCCAGAAGAGAAGGCCATTCCACAAGGCAGTGTCCGCAAAGGCT	5	0.125	No Hit
GGCTTGTTGTGAATCCACTTCACTACTTTGACGCCATTCTGAGCAGTGGC	5	0.125	No Hit
GGCTGGTGGCGCAGCAAGTCCACAACATCTTTGGAGAACTGCTTTGTCCT	5	0.125	No Hit
GGTCATAGTGCTGAGAACTGGGAATCACTGGCTTCATTTAAAAGATATTG	5	0.125	No Hit
ACCCAAACATGCTTCCAACAGTGAACAGTAACAGTAAAGCTAGACACTAT	5	0.125	No Hit
CGGTGCCTGAGATCACTGAGTTCTAATGCAAAATTAACATCAGTTGACAC	5	0.125	No Hit
TTCCCCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTG	5	0.125	No Hit
GTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAGAATCAG	5	0.125	No Hit
GCGGTGTTCAAAAGGTTTGGAGACATACTCCTTGAGGCGATCAGCTGGCT	5	0.125	No Hit
GACACACACATCTTCACATTGAGAGCTTCCCAGATTTACAGCCAATTTTT	5	0.125	No Hit
GGTAGCTTATTTGGAAGACAGTCCCCTCTCTCTTTTTCTTCTGTGCAGGA	5	0.125	No Hit
CTCTGTCTTGCGTAATTTCTAGGTCACCAAACTCCGCAGCATAGCAATCT	5	0.125	No Hit
GCCCATTCACTGTACACAACTCTTCTTTGTTGCCCACAGAACCTGACCTT	5	0.125	No Hit
CCGGCCAAAGTGGTGGTGGTAAGAGGGAATGAACTTGTTAAAGGGCATTC	5	0.125	No Hit
TCTCAATACACAATATGTCATTCAAATGCTGACTTAAATGGATGACATCG	5	0.125	No Hit
GCTCCATCTGGGGCAGGTCATAGTGCTGAGAACTGGGAATCACTGGCTTC	5	0.125	No Hit
TGGCTGCCAATTTGACTCTATTTTTGGGCTGCTTTTTTGCTGGGCTTTCC	5	0.125	No Hit
CGGGTTCTGGCACCTCCAGGATCTGCCTGCTGACTGCAGGCTGGTCTTCA	5	0.125	No Hit
CGCTCTGAGAGCTTCCAATTGAGTTTTCACATCTTGTGACATCTTGTAAC	5	0.125	No Hit
TCTCTCAACCTTTAATTTTGTGACATTTTTATCAAAATGCTGGCCATGAA	5	0.125	No Hit
CCCCATTCACTGTACACAACTCTTCTTTGTTGCCCACAGAACCTGACCTT	5	0.125	No Hit
GACAGTTTTCCCCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTA	5	0.125	No Hit
TCCCTGTTCACGGATTGCAATCATGTCTGTTAATTTATATAAATCAGACA	5	0.125	No Hit
GCTGTCTTGGTTTATAAAGCGGAGAATAGCGCTGCAGCCTGTGATACTTA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0375	0.0	0.0	0.0
12-13	0.0	0.075	0.0	0.0	0.0
14-15	0.0	0.1375	0.0	0.0	0.0
16-17	0.0	0.2	0.0	0.0	0.0
18-19	0.0	0.2	0.0	0.0	0.0
20-21	0.0	0.2	0.0	0.0	0.0
22-23	0.0	0.2	0.0	0.0	0.0
24-25	0.0	0.2	0.0	0.0	0.0
26-27	0.0	0.21250000000000002	0.0	0.0	0.0
28-29	0.0	0.375	0.0	0.0	0.0
30-31	0.0	0.375	0.0	0.0	0.0
32-33	0.0	0.375	0.0	0.0	0.0
34-35	0.0	0.375	0.0	0.0	0.0
36-37	0.0	0.375	0.0	0.0	0.0
38-39	0.0	0.375	0.0	0.0	0.0
40-41	0.0	0.375	0.0	0.0	0.0
42-43	0.0	0.375	0.0	0.0	0.0
44-45	0.0	0.375	0.0	0.0	0.0
46-47	0.0	0.375	0.0	0.0	0.0
48-49	0.0	0.375	0.0	0.0	0.0
50-51	0.0	0.375	0.0	0.0	0.0
52-53	0.0	0.425	0.0	0.0	0.0
54-55	0.0	0.425	0.0	0.0	0.0
56-57	0.0	0.425	0.0	0.0	0.0
58-59	0.0	0.425	0.0	0.0	0.0
60-61	0.0	0.425	0.0	0.0	0.0
62-63	0.0	0.425	0.0	0.0	0.0
64-65	0.0	0.425	0.0	0.0	0.0
66-67	0.0	0.425	0.0	0.0	0.0
68-69	0.0	0.55	0.0	0.0	0.0
70-71	0.0	0.8125	0.0	0.0	0.0
72-73	0.0	0.875	0.0	0.0	0.0
74-75	0.0	0.875	0.0	0.0	0.0
76-77	0.0	0.875	0.0	0.0	0.0
78-79	0.0	0.875	0.0	0.0	0.0
80-81	0.0	0.875	0.0	0.0	0.0
82-83	0.0	0.975	0.0	0.0	0.0
84-85	0.0	1.025	0.0	0.0	0.0
86-87	0.0	1.025	0.0	0.0	0.0
88-89	0.0	1.025	0.0	0.0	0.0
90-91	0.0	1.025	0.0	0.0	0.0
92-93	0.0	1.0375	0.0	0.0	0.0
94-95	0.0	1.05	0.0	0.0	0.0
96-97	0.0	1.05	0.0	0.0	0.0
98-99	0.0	1.05	0.0	0.0	0.0
100-101	0.0	1.05	0.0	0.0	0.0
102-103	0.0	1.05	0.0	0.0	0.0
104-105	0.0	1.05	0.0	0.0	0.0
106-107	0.0	1.05	0.0	0.0	0.0
108-109	0.0	1.1125	0.0	0.0	0.0
110-111	0.0	1.175	0.0	0.0	0.0
112-113	0.0	1.175	0.0	0.0	0.0
114-115	0.0	1.175	0.0	0.0	0.0
116-117	0.0	1.175	0.0	0.0	0.0
118-119	0.0	1.175	0.0	0.0	0.0
120-121	0.0	1.175	0.0	0.0	0.0
122-123	0.0	1.175	0.0	0.0	0.0
124-125	0.0	1.175	0.0	0.0	0.0
126-127	0.0	1.175	0.0	0.0	0.0
128-129	0.0	1.175	0.0	0.0	0.0
130-131	0.0	1.175	0.0	0.0	0.0
132-133	0.0	1.175	0.0	0.0	0.0
134-135	0.0	1.175	0.0	0.0	0.0
136-137	0.0	1.175	0.0	0.0	0.0
138-139	0.0	1.2	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTTAG	20	0.0052467096	55.77518	135-139
TGCTTGC	20	0.008976931	48.759434	125-129
GTCCCAT	25	6.71581E-4	44.943478	115-119
AAAGTCC	25	0.0012356602	39.75769	105-109
TCCCATC	25	0.0026702345	34.031273	115-119
CATAGTC	25	0.0035896276	32.05271	110-114
AACTTTA	30	0.004659267	30.358295	80-84
CCCATCT	25	0.0076684826	27.473753	115-119
AGGTTCT	25	9.765697E-4	25.8425	40-44
GGTTCTC	30	0.0028226022	21.535416	40-44
>>END_MODULE
Read 9816966 spots for SRR26281820.sra
Written 9816966 spots for SRR26281820.sra
Read 9816966 spots for SRR26281820.sra
Written 9816966 spots for SRR26281820.sra
Read 9816966 spots for SRR26281820.sra
Written 9816966 spots for SRR26281820.sra
Read 9816966 spots for SRR26281820.sra
Written 9816966 spots for SRR26281820.sra
Read 9816966 spots for SRR26281820.sra
Written 9816966 spots for SRR26281820.sra
Read 9816966 spots for SRR26281820.sra
Written 9816966 spots for SRR26281820.sra
Read 9816966 spots for SRR26281820.sra
Written 9816966 spots for SRR26281820.sra
Rejected 17052 READS because READLEN < 1
Read 9816971 spots for SRR26281820.sra
Written 9816971 spots for SRR26281820.sra
SRR ids: ['SRR26281820.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_fcmmayrc
SRR26281820.sra spots: 78535733
blocks: [[1, 9816966], [9816967, 19633932], [19633933, 29450898], [29450899, 39267864], [39267865, 49084830], [49084831, 58901796], [58901797, 68718762], [68718763, 78535733]]
SRR26281820 file size 24560904
SRR26281820 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR26281820 SRR26281820_1.fastq SRR26281820_2.fastq
Input file:	SRR26281820_1.fastq
Paired file:	SRR26281820_2.fastq
trimmed:	SRR26281820-trimmed-pair1.fastq, SRR26281820-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Mon Nov 10 18:28:12 2025 >> started

Mon Nov 10 18:30:46 2025 >> done (154.362s)
78521454 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
78521454 (100.00%) read pairs available; of these:
  202209 ( 0.26%) trimmed read pairs available after processing
78319245 (99.74%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 35	    1771	  0.00%
 36	    2189	  0.00%
 37	    2600	  0.00%
 38	    3225	  0.00%
 39	    4216	  0.01%
 40	    5069	  0.01%
 41	    5610	  0.01%
 42	    6085	  0.01%
 43	    5887	  0.01%
 44	    5698	  0.01%
 45	    5759	  0.01%
 46	    6359	  0.01%
 47	    7628	  0.01%
 48	    9311	  0.01%
 49	   11173	  0.01%
 50	   12647	  0.02%
 51	   14376	  0.02%
 52	   15029	  0.02%
 53	   15010	  0.02%
 54	   15005	  0.02%
 55	   15265	  0.02%
 56	   15948	  0.02%
 57	   17642	  0.02%
 58	   20582	  0.03%
 59	   23454	  0.03%
 60	   26143	  0.03%
 61	   29564	  0.04%
 62	   32876	  0.04%
 63	   34958	  0.04%
 64	   36297	  0.05%
 65	   37246	  0.05%
 66	   38004	  0.05%
 67	   39810	  0.05%
 68	   40797	  0.05%
 69	   44142	  0.06%
 70	   48667	  0.06%
 71	   54385	  0.07%
 72	   61508	  0.08%
 73	   69084	  0.09%
 74	   76108	  0.10%
 75	   82123	  0.10%
 76	   86252	  0.11%
 77	   88088	  0.11%
 78	   89930	  0.11%
 79	   92892	  0.12%
 80	   98853	  0.13%
 81	  107537	  0.14%
 82	  122274	  0.16%
 83	  136493	  0.17%
 84	  153111	  0.19%
 85	  167541	  0.21%
 86	  177317	  0.23%
 87	  182547	  0.23%
 88	  186474	  0.24%
 89	  190539	  0.24%
 90	  193825	  0.25%
 91	  206295	  0.26%
 92	  223309	  0.28%
 93	  248628	  0.32%
 94	  273375	  0.35%
 95	  299179	  0.38%
 96	  320162	  0.41%
 97	  333143	  0.42%
 98	  340182	  0.43%
 99	  338124	  0.43%
100	  343905	  0.44%
101	  349268	  0.44%
102	  366248	  0.47%
103	  391009	  0.50%
104	  416059	  0.53%
105	  447294	  0.57%
106	  472856	  0.60%
107	  483881	  0.62%
108	  489030	  0.62%
109	  493857	  0.63%
110	  490184	  0.62%
111	  490419	  0.62%
112	  495357	  0.63%
113	  507844	  0.65%
114	  524382	  0.67%
115	  549616	  0.70%
116	  566334	  0.72%
117	  579906	  0.74%
118	  590821	  0.75%
119	  588930	  0.75%
120	  583746	  0.74%
121	  578791	  0.74%
122	  574352	  0.73%
123	  571019	  0.73%
124	  577433	  0.74%
125	  589845	  0.75%
126	  592352	  0.75%
127	  610035	  0.78%
128	  614326	  0.78%
129	  614726	  0.78%
130	  611927	  0.78%
131	  605865	  0.77%
132	  592139	  0.75%
133	  587968	  0.75%
134	  578918	  0.74%
135	  579060	  0.74%
136	  583169	  0.74%
137	  594881	  0.76%
138	  596668	  0.76%
139	  596329	  0.76%
140	  593099	  0.76%
141	  586520	  0.75%
142	  567937	  0.72%
143	  555506	  0.71%
144	  549546	  0.70%
145	  550979	  0.70%
146	  578930	  0.74%
147	  635853	  0.81%
148	 1192172	  1.52%
149	 5187154	  6.61%
150	21614615	 27.53%
151	18783074	 23.92%
78521454 reads passed initial QC


criterion=sequence-density
sequence-density=1.72
sequence-density-rank=1
fanout-score=2.13
fanout-score-rank=32
prefix-density=1.78
prefix-fanout=2.1
sequence=GGCATCAAGGAGCAGGACATCAACAAGGATCTGGTGATCTAAGTTCAAGCCTCCTGCAGCAGTCCTGGACTCTCTCCCCCTGATGTCCCCACCCACTTCCACTACCCCCTTGTTTAAAATGTTTGGATGGTTGGCTGTTCTGCCTGGGGATAAGGTGCTAACATAGATTTAACTGAATACATTAACGGTGCTAAAAA


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=29
fanout-score=46.57
fanout-score-rank=1
prefix-density=0.95
prefix-fanout=1.6
sequence=CAGAAGCAGCCGCCGGGATGGACGTGTTTCTCATGATCCGGCGCCACAAGACCACCATCTTTACGGACGCCAAGGAGTCGAGCACGGTGTTCGAACTGAAGCGCATCGTCGAGGGCATCCTCAAGCGGCCGCCAGAGGAGCAGCGGCTGTACAAGGACGACCAGCTCCTTGATGATGGAAAAACTCTGGGCGAATGTGGCTTCACCAGTCAGACAGCAAGGCCACAGGCCCCAGCCACAGTGGGCCTGGCCTTTCGAGCAGATGACACCTTTGAAGCGCTGCGTATTGAACCCTTCTCCAGCCCTCCGGAGCTTCCAGATGTGATGAAGCCACAGGATTCTGGAGGCAGTGCCAATGAACAAGCTGTGCAGTGAGGGCCCCAGGCCTACCCCCTAGAAACCCATTCCCCCAATAA


criterion=sequence-density
sequence-density=1.47
sequence-density-rank=1
fanout-score=2.03
fanout-score-rank=33
prefix-density=1.49
prefix-fanout=2.0
sequence=AAGCAGCCGGCCCATTCCTCCAGGGCAATGTACTTGTCATTGTCTAGGTCACAGGTCTCAAAGAAGCGAGTGGTGCAATGTTCCATGGGAATGAGGGGAGCGCGCAGTGGGGCCAGCTCCGTGTGGGACAGGTACCCATCAATCGGGTGCTGATCCAGCTGGCCAAACTGCCAGTGGACAGGGAAGATGTACATGTTGTAGTTCTTCTCAAAGTCTCGGGCCAGCAGCTCCACAGGGTGGTCTCCAGCCTCCAGGCGCTTCTCGTTCTCGTGGATCTTCTTCACACGCAGTTTCTGCTTCTCAGTGAGGAGGTTGTTGCCCTCATCTCTCTCGTACAAGGTGACCAGGACGTTTTTGAGCCAGTCACGCATGCGCAGAGGGAATTCGGTCAGCTCAGAATCCAGGCAGGGGGCAATGTATTTGCATGGTCCGATGTAGTCCAGGTGGAGCTTGTGGCCCTTCTTGGTGCCCTCCAGGGTGCACTTGGTCGCAAAGAAGTGGCAGGAAGAGT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=35
fanout-score=99.59
fanout-score-rank=1
prefix-density=0.53
prefix-fanout=2.0
sequence=TGGGGGCAGGGTGGCTCCATCCAAGGAGTCTCAGCAACAGGAACCCCACAAGGCAACAGTCTACTAATGATTACTATAGAGTTGGGAGACAGGGCTGCTCCACAGACACATGAATAGGTCAAATCTTTTATAAATAAACATCCAGTGAAGAGAAAAATGACAACTCTAATTCATTCTTATACACAAGGCGCACTCTAGGGCGATGGGTGGGGTGACCAGAGGCTGAAATCGTCACTGGTCAGGGGCCTGGGTGGGAGATGAGTGGGCCCAGATGTCCACAGCACGGGGCAGTAGGGGCAAGGCGGGGCAACAGACAATGGGCACTAGGACACTGCACATTTACAAGACCAACTACTGAGGGGGCAGCTGCCCGTGCATGTGTCAGGCTGTTCCTTCTGGAATGAGGAGGGGTGATCTTTACATCCTATCCTGTGACTGGTGGTGGAAAAAGACTACTTTGTGGAGAGGATTAGGGCCACGATGAGACCGTAGAGGCCAAGCACCTCCGCAA
SRR26281820 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Nov 10 18:31:15
                             Started mapping on |	Nov 10 18:31:15
                                    Finished on |	Nov 10 18:41:42
       Mapping speed, Million of reads per hour |	450.84

                          Number of input reads |	78521454
                      Average input read length |	274
                                    UNIQUE READS:
                   Uniquely mapped reads number |	71101051
                        Uniquely mapped reads % |	90.55%
                          Average mapped length |	273.23
                       Number of splices: Total |	81634030
            Number of splices: Annotated (sjdb) |	79285123
                       Number of splices: GT/AG |	80576386
                       Number of splices: GC/AG |	555098
                       Number of splices: AT/AC |	52396
               Number of splices: Non-canonical |	450150
                      Mismatch rate per base, % |	0.42%
                         Deletion rate per base |	0.02%
                        Deletion average length |	1.67
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.68
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	7406648
             % of reads mapped to multiple loci |	9.43%
        Number of reads mapped to too many loci |	7613
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.01%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	13782	13782	13782
N_multimapping	7406648	7406648	7406648
N_noFeature	7879861	8818741	69618349
N_ambiguous	1090612	539908	10743
UnstrandedReadsAssigned:62130578 PositiveStrandReadsAssigned:61742402 NegativeStrandReadsAssigned:1471959
Dataset is classified positive stranded
MeadianReadLen=150 20thPercentileLength=121 echo kmer=117
SRR26281820 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 31,715
[index] number of k-mers: 49,915,185
[index] number of equivalence classes: 70,351
[quant] running in paired-end mode
[quant] will process pair 1: SRR26281820-trimmed-pair1.fastq
                             SRR26281820-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 78,521,454 reads, 66,857,324 reads pseudoaligned
[quant] estimated average fragment length: 176.706
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,174 rounds

  31716 SRR26281820.ke.tsv
  32883 SRR26281820.se.tsv
  64599 total
==> SRR26281820.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	779.294	126748	3671.84
ENSRNOT00000040993.4	1039	863.294	188683	4934.21
ENSRNOT00000050156.3	1545	1369.29	1.13365e+06	18690.7
ENSRNOT00000043693.3	684	508.546	267498	11875
ENSRNOT00000046201.3	204	65.781	61618	21147.1
ENSRNOT00000046108.3	681	505.546	315237	14077.3
ENSRNOT00000049683.3	784	608.462	377814	14018.1
ENSRNOT00000041241.3	348	182.094	26827	3325.99
ENSRNOT00000044582.3	297	137.562	23416.1	3842.89

==> SRR26281820.se.tsv <==
ENSRNOG00000046319	2
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	102
ENSRNOG00000058808	0
ENSRNOG00000061316	2716
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	60
SRR26281820 completed mapping pipeline successfully
