Starting /dee2/code/volunteer_pipeline.sh SRR26281810
    current disk space = 693904824
    free memory = 199848868 
SRR26281810 SRAfilesize
022069b80343a05754afa95e8249b959  SRR26281810.sra
SRR26281810.sra file validated
SRR26281810 is paired end
SRR26281810 is conventional basespace
SRR26281810 read1 length is 48-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR26281810_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	48-151
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.2315	37.0	37.0	37.0	37.0	37.0
2	36.14675	37.0	37.0	37.0	37.0	37.0
3	36.208	37.0	37.0	37.0	37.0	37.0
4	36.314	37.0	37.0	37.0	37.0	37.0
5	36.329	37.0	37.0	37.0	37.0	37.0
6	36.347	37.0	37.0	37.0	37.0	37.0
7	36.241	37.0	37.0	37.0	37.0	37.0
8	36.329	37.0	37.0	37.0	37.0	37.0
9	36.223	37.0	37.0	37.0	37.0	37.0
10-14	36.3656	37.0	37.0	37.0	37.0	37.0
15-19	36.3768	37.0	37.0	37.0	37.0	37.0
20-24	36.359899999999996	37.0	37.0	37.0	37.0	37.0
25-29	36.2715	37.0	37.0	37.0	37.0	37.0
30-34	36.2275	37.0	37.0	37.0	37.0	37.0
35-39	36.249900000000004	37.0	37.0	37.0	37.0	37.0
40-44	36.193200000000004	37.0	37.0	37.0	37.0	37.0
45-49	36.10511210605303	37.0	37.0	37.0	37.0	37.0
50-54	36.143108441107444	37.0	37.0	37.0	37.0	37.0
55-59	36.1032032032032	37.0	37.0	37.0	37.0	37.0
60-64	36.15388884054901	37.0	37.0	37.0	37.0	37.0
65-69	36.04635020867191	37.0	37.0	37.0	37.0	37.0
70-74	36.06904655452813	37.0	37.0	37.0	37.0	37.0
75-79	36.04556138521916	37.0	37.0	37.0	37.0	37.0
80-84	35.98927513981488	37.0	37.0	37.0	37.0	37.0
85-89	36.03064566472402	37.0	37.0	37.0	37.0	37.0
90-94	35.96227985713365	37.0	37.0	37.0	37.0	37.0
95-99	36.00733012635354	37.0	37.0	37.0	37.0	37.0
100-104	35.85575781794728	37.0	37.0	37.0	37.0	37.0
105-109	35.801796462560624	37.0	37.0	37.0	37.0	37.0
110-114	35.84524656498026	37.0	37.0	37.0	37.0	37.0
115-119	35.83875732015302	37.0	37.0	37.0	37.0	37.0
120-124	35.75982526183142	37.0	37.0	37.0	37.0	37.0
125-129	35.68110548783845	37.0	37.0	37.0	37.0	37.0
130-134	35.693649226298874	37.0	37.0	37.0	37.0	37.0
135-139	35.60215819001468	37.0	37.0	37.0	37.0	37.0
140-144	35.59128023943792	37.0	37.0	37.0	37.0	37.0
145-149	35.492803947679995	37.0	37.0	37.0	37.0	37.0
150-151	35.84666584282979	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	2.0
26	3.0
27	10.0
28	21.0
29	30.0
30	48.0
31	65.0
32	81.0
33	121.0
34	165.0
35	389.0
36	2547.0
37	518.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.1	23.400000000000002	13.15	30.349999999999998
2	27.64838467317806	20.18532431755572	28.95066366140746	23.215627347858753
3	20.825	25.525	30.925000000000004	22.725
4	20.325	34.8	25.424999999999997	19.45
5	22.875	34.5	23.775	18.85
6	22.125	33.525	21.8	22.55
7	18.875	21.525	38.1	21.5
8	22.875	26.125	27.3	23.7
9	21.425	22.75	31.8	24.025
10-14	23.150000000000002	27.005000000000003	26.795	23.05
15-19	21.245	27.045	28.405	23.305
20-24	23.815	27.860000000000003	25.580000000000002	22.745
25-29	22.2	26.99	27.075	23.735
30-34	22.945	28.835	26.1	22.12
35-39	22.63	26.790000000000003	28.475	22.105
40-44	23.49	26.375	28.689999999999998	21.445
45-49	22.28722872287229	27.93779377937794	26.972697269726975	22.802280228022802
50-54	21.917534027221777	28.813050440352285	27.12169735788631	22.147718174539634
55-59	21.686686686686688	26.16116116116116	29.68968968968969	22.46246246246246
60-64	23.30092221331195	27.596230954290295	27.18524458700882	21.917602245388935
65-69	22.242305568107646	28.372746899633476	26.705829191143245	22.679118341115633
70-74	22.54358560919077	28.217273002116293	26.216869898216267	23.02227149047667
75-79	22.96044968856029	27.30541348052869	26.535676305261557	23.198460525649466
80-84	23.35658638423545	26.890371200162942	29.125719232140128	20.62732318346148
85-89	20.830970668591164	27.712768699417495	27.795247177689568	23.66101345430177
90-94	21.99864463326904	28.426210707397175	27.936193504665592	21.638951154668195
95-99	23.187480038326413	29.287767486426063	26.03002235707442	21.49473011817311
100-104	23.551002519995617	29.220992659143203	26.021693875314998	21.206310945546182
105-109	22.14213312309945	29.412095956751887	27.249690280436983	21.196080639711678
110-114	22.93486545113443	29.213812510992554	25.995192589552673	21.85612944832034
115-119	22.592319960741015	28.0149674886517	27.426082689240584	21.966629861366705
120-124	24.276938827745074	27.30995648835424	26.78525723061172	21.627847453288968
125-129	22.99005108900242	29.725732723850495	24.011831137402528	23.272385049744553
130-134	21.690831710308014	29.58955493039025	26.386784967178823	22.332828392122916
135-139	23.364914425427873	26.85666259168704	28.002750611246945	21.77567237163814
140-144	21.300016069419893	30.604210188012214	25.606620600996305	22.489153141571588
145-149	22.567960335100018	28.415113694648657	26.440417165327407	22.576508804923918
150-151	21.035308198683424	13.494913225613406	34.590065828845	30.879712746858168
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	1.0
25	14.0
26	23.5
27	15.5
28	11.5
29	21.0
30	23.0
31	26.0
32	33.5
33	38.5
34	49.0
35	71.0
36	93.0
37	90.0
38	122.0
39	145.5
40	152.5
41	185.0
42	216.5
43	246.5
44	231.0
45	220.0
46	259.0
47	250.5
48	203.5
49	178.5
50	163.5
51	141.0
52	126.5
53	129.0
54	104.0
55	68.5
56	59.5
57	67.0
58	72.0
59	50.0
60	31.0
61	31.0
62	27.0
63	14.0
64	4.0
65	5.5
66	3.5
67	6.0
68	6.5
69	6.5
70	7.0
71	3.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.17500000000000002
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
45-49	2.0
50-54	2.0
55-59	4.0
60-64	8.0
65-69	8.0
70-74	14.0
75-79	24.0
80-84	33.0
85-89	48.0
90-94	58.0
95-99	110.0
100-104	87.0
105-109	131.0
110-114	154.0
115-119	135.0
120-124	144.0
125-129	189.0
130-134	170.0
135-139	139.0
140-144	127.0
145-149	417.0
150-152	1996.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	35.11680482290881	11.65
2	19.140919366993216	12.7
3	15.373021853805577	15.299999999999999
4	10.92690278824416	14.499999999999998
5	6.556141672946496	10.875
6	4.069329314242653	8.1
7	2.7128862094951014	6.3
8	1.5825169555388092	4.2
9	1.4318010550113038	4.275
>10	3.089675960813866	12.1
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGAGGTATCCACTTATGAGTGGCCTCTCACCGCTGTTGGAGAGACCTGA	26	0.65	No Hit
GAAAAAACAGAAATTCCAATACCAGTAAAGAAGGGGAACCTGTCCTGTAA	21	0.525	No Hit
CTTCTCTGTCTGTCTCCGTTTGATAAGGGTAAGGTTTAAGGAAAAGAGAT	19	0.475	No Hit
GGAAAAGAGATGGTCTCGATGTCATCCATTTAAGTCAGCATTTGAATGAC	14	0.35000000000000003	No Hit
GGCAAATTCTGGATCTGCTACAAAAAACACAAACTTTAAAGGTTTACAGG	14	0.35000000000000003	No Hit
GGGGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCTTCTG	13	0.325	No Hit
GGAAAGTTCCTGGCTTCTAGGTGACTTATTGGTGTGCCTTGTTTTTTATG	13	0.325	No Hit
CTCAATGTGAAGATGTGTGTGTCCATGTTAGTTCATCTTGACGTCTTTAA	12	0.3	No Hit
GCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCCCATTTTACCATGG	12	0.3	No Hit
CCTCCATCATTTGTTTAAGCAAATGTATTTGTGGTTGTTCAAGAAGCCAA	12	0.3	No Hit
GCCAGCTGATCGCCTCAAGGCAAGTCAGTCCTCTGTTCAGCTCTCAGTCT	12	0.3	No Hit
CTTTTCTTTTGCACCTGTGACCAAGCTCTAACTTCCAGTTTGAACACAGA	12	0.3	No Hit
CCAACTCTCTCCACCTAAATGTGGCACCTCCGGTTTGTTTAAAAGGCTCA	12	0.3	No Hit
TGGAAAGCCCAGCAAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGCC	12	0.3	No Hit
GGGAAATGTTTGTTTCCAACTTTCAGATGAACTTTTTCTTACTTTATTTC	12	0.3	No Hit
CTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCTTCTGCTCCTCC	12	0.3	No Hit
CCTAAGGCTAAGCTTGCCTGCTCAGACTAAAGGTTTGCAAGCATAGATGG	11	0.27499999999999997	No Hit
GTGTGAGTTGCCTCTTTTGCAGACGTCCTAAGGCTAAGCTTGCCTGCTCA	11	0.27499999999999997	No Hit
ACTCCATTAAGGAATCTGTATTATATTTACCAAATTTTTCTAGACTATTG	11	0.27499999999999997	No Hit
GAGATAATGAGACACTTTTTCTGGGTGGAAGGATGTACCCAGTTAGATTT	11	0.27499999999999997	No Hit
GCCACTGCTCAGAATGGCGTCAAAGTAGTGAAGTGGATTCACAACAAGCC	11	0.27499999999999997	No Hit
AGTGGATTGAGTCCCATTGAGCAGCCTCCATCATTTGTTTAAGCAAATGT	11	0.27499999999999997	No Hit
CGCCTCCCTGGGATCTTCGCCGCCCGAGCTGGACATCATGCCACAGGAAG	10	0.25	No Hit
ATCGGAATGTAAGCGGAGAAGGAGCTGGTGGCTGAGGGTGGAAGAATGAA	10	0.25	No Hit
CTTTACTGTGAAGACTGTCTAAACAAGCCAGCAAGAAATAGTATAATTGA	10	0.25	No Hit
CATCACCATTTTGGAAAGCAGTGCCGGGTTTCAGACTATGGATATTCCAA	10	0.25	No Hit
ATTTCGTCTCCAAGACTATGATGTCAGTTCAGTCTCAGCTTTGACACAGA	10	0.25	No Hit
CATTTGTATCCGAAAAAGAGAACGAACTCAGGATGAAATAGAAAGGACAA	10	0.25	No Hit
GGATATTCCAAGCTGATTGAGCTACTAGAAGCCGTGCCTCATGTGTTGCA	10	0.25	No Hit
AATACGCACACTGCTCCAGCTACTGTGGTTCTTGTGTCAACTGATGTTAA	10	0.25	No Hit
CCCGGGCTACTGATTAGCAAGGATGCAGTGGAAAGCCCAGCAAAAAAGCA	10	0.25	No Hit
GCTACAAAAAACACAAACTTTAAAGGTTTACAGGAGCTGTGCCGAATGGA	10	0.25	No Hit
AGGCAAAGCTGCTTCTAAATTTGGGTCACCAGAAGTTGCAGTCACTGGAC	10	0.25	No Hit
CGAAAGAGTACATGGAGAACAAGAAAGCTGCTGTGGAGTTAAAGGATATA	10	0.25	No Hit
TCTCGATGTCATCCATTTAAGTCAGCATTTGAATGACATATTGTGTATTG	10	0.25	No Hit
GTTTTATCTGTAATTTGAAGGCCAAGGTTGATCCTTGTTCTTGTGATTTA	10	0.25	No Hit
AGGATGTACCCAGTTAGATTTATCTGTTGAAGAGACAAAAGTTATCACCA	10	0.25	No Hit
CGTCAAAGTAGTGAAGTGGATTCACAACAAGCCCCCACCTCCCAACACTG	10	0.25	No Hit
ACTGAAGATTCCTTGCCACACTCTACTCTATGTTTATAACCTGCCGGCAA	10	0.25	No Hit
GCCTGCTAGCCCGCGCCTCCCTGGGATCTTCGCCGCCCGAGCTGGACATC	10	0.25	No Hit
AGAAAATGGATTCTTATTTCATGGCCAGCATTTTGATAAAAATGTCACAA	10	0.25	No Hit
GAAAAAGAGAACGAACTCAGGATGAAATAGAAAGGACAAAGCAGTTCTCC	9	0.22499999999999998	No Hit
AGCATAGATGGGACTATGGACTTTGAAAACACTAGGTTTCACTAAAGTTA	9	0.22499999999999998	No Hit
GGCTTCTAAGCAGTGATAAAGTTGCTGTTAGGAGTGTGAGTTGCCTCTTT	9	0.22499999999999998	No Hit
AGAAACACTGCAGCCCAAAACTTATGGCTACAGTAGTGTGGAGGAGCTAC	9	0.22499999999999998	No Hit
GTTCATTTGACCTTTCCCCTGTCAATCATGAAGACCAGCCTGCAGTCAGC	9	0.22499999999999998	No Hit
ACTCACAGTACTCCTCTCAACCCCTGTGAATATGGTTTCATGACTTTGAC	9	0.22499999999999998	No Hit
GTGAACATTGCCACTGCTCAGAATGGCGTCAAAGTAGTGAAGTGGATTCA	9	0.22499999999999998	No Hit
CAAGAAGCCAAACATGTCCTTAAACCTGTGGGTCACACACTGGGGCATGA	9	0.22499999999999998	No Hit
GATTTACCAAGTTGCTGGAACTTTTGGAAGCCATACCTGAGATTTTACAA	9	0.22499999999999998	No Hit
AAATATGTAGGAGAAACACTGCAGCCCAAAACTTATGGCTACAGTAGTGT	9	0.22499999999999998	No Hit
CAAAAATAGAGTCAAATTGGCAGCCAACTTTTCTTTTGCACCTGTGACCA	9	0.22499999999999998	No Hit
AAGACATTCTTGAAGGTCCAGTTCCTATTTCACAGATTGACTAAATGTCC	9	0.22499999999999998	No Hit
ATAAACTCCGTCAGAGCCTGCGAAGGTTTGCAAATACGCACACTGCTCCA	9	0.22499999999999998	No Hit
GTTTGATAAGGGTAAGGTTTAAGGAAAAGAGATGGTCTCGATGTCATCCA	9	0.22499999999999998	No Hit
CAGGAAGTGGATTGAGTCCCATTGAGCAGCCTCCATCATTTGTTTAAGCA	9	0.22499999999999998	No Hit
GACGTCCTAAGGCTAAGCTTGCCTGCTCAGACTAAAGGTTTGCAAGCATA	9	0.22499999999999998	No Hit
GGGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCTTCTGC	9	0.22499999999999998	No Hit
CCAAGCTGATTGAGCTACTAGAAGCCGTGCCTCATGTGTTGCAGATTCTT	9	0.22499999999999998	No Hit
CCGCTTCAGTGCCCACACTGAAAAACACAGGTGTGAGGGAGACACTTCAC	9	0.22499999999999998	No Hit
CTCGAGCTGTTCCTCTGTGTAATCGCTGTGGAACAAAAGGGATGGAAAAG	8	0.2	No Hit
AAATGTGGCACCTCCGGTTTGTTTAAAAGGCTCACTTTACTGTGAAGACT	8	0.2	No Hit
CCTCAAGGCAAGTCAGTCCTCTGTTCAGCTCTCAGTCTTGGTCTCCGAGG	8	0.2	No Hit
TGGTCTCCGAGGAGTATGTCTCCAAACCTTTTGAACACCGCCTCGCCACT	8	0.2	No Hit
CCTGCTCAGACTAAAGGTTTGCAAGCATAGATGGGACTATGGACTTTGAA	8	0.2	No Hit
CCTCCCTGGGATCTTCGCCGCCCGAGCTGGACATCATGCCACAGGAAGAG	8	0.2	No Hit
CGGAAGGGCGCCCATGGAGCGGGGTCTGCCGGGCGAACGCCAGAGTATGG	8	0.2	No Hit
GCCTCATGTGTTGCAGATTCTTGGAATGGGCTCCAAGCGTCTACTGACCC	8	0.2	No Hit
CAGATTTGAAGAGTTCATTTCCGATCTACCCCCCAGGTTACCACTGAAGA	8	0.2	No Hit
ATTTTGGAAAGCAGTGCCGGGTTTCAGACTATGGATATTCCAAGCTGATT	8	0.2	No Hit
AGAAAGGCCTGCTAGCCCGCGCCTCCCTGGGATCTTCGCCGCCCGAGCTG	8	0.2	No Hit
CTCAAGTTCATAGTCTCCTGCAGACCCACGAGGGCACTGTGCCTTTGCTG	8	0.2	No Hit
AGCTAGCCTTTGCGGACACTGCCTTGTGGAATGGCCTTCTCTTCTGGGTG	8	0.2	No Hit
TATGGATATTCCAAGCTGATTGAGCTACTAGAAGCCGTGCCTCATGTGTT	8	0.2	No Hit
GGGACTTTCTGGTGGCTATCTCTAGTTCTGCATTCCCTCACTGCTCCACA	8	0.2	No Hit
GCCCAGCATCTGCTTCTGCTCCTCCCAAACCCTCCTCAGAAGCCCCCGGG	8	0.2	No Hit
CACCGCTGTTGGAGAGACCTGACATTTACAATGGATTGTATCTGTTGGGC	8	0.2	No Hit
CTCCACCTAAATGTGGCACCTCCGGTTTGTTTAAAAGGCTCACTTTACTG	8	0.2	No Hit
GTCAGCAACAACTGTTGTACAGAGAATCCGTGAGAAGTTTTTTAGAGGAC	8	0.2	No Hit
TGCTCGAACAAGGACTTCAGCGAACTTGTATTTGATCCAGACTCTTACAA	8	0.2	No Hit
GGAATCTGTATTATATTTACCAAATTTTTCTAGACTATTGTGAATTGTGG	8	0.2	No Hit
GTTTTTTAGAGGACACAGAGAAGCAGAGTTTATCTGTGTGTGTGACATCA	7	0.17500000000000002	No Hit
CCATTTTGGAAAGCAGTGCCGGGTTTCAGACTATGGATATTCCAAGCTGA	7	0.17500000000000002	No Hit
GCCTTGTTACATCATGCTAACCAGCTGATCAGATTTGAAGAGTTCATTTC	7	0.17500000000000002	No Hit
TCCTAAGGCTAAGCTTGCCTGCTCAGACTAAAGGTTTGCAAGCATAGATG	7	0.17500000000000002	No Hit
ATTTCATGCTTCCCTTCTCTGTCTGTCTCCGTTTGATAAGGGTAAGGTTT	7	0.17500000000000002	No Hit
GTAAAGAAGGGGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATC	7	0.17500000000000002	No Hit
TATGAGTGGCCTCTCACCGCTGTTGGAGAGACCTGACATTTACAATGGAT	7	0.17500000000000002	No Hit
GTTCGCACCTCAAGTTCATAGTCTCCTGCAGACCCACGAGGGCACTGTGC	7	0.17500000000000002	No Hit
TGTTAGTTCATCTTGACGTCTTTAATAGTGTCTAGCTTTACTGTTACTGT	7	0.17500000000000002	No Hit
CTCCTCCAAGAAGCCTTCTCCAAGCATGGCCAGGTGAAGAGTGTTGAGCT	7	0.17500000000000002	No Hit
AGGGGACTGTCTTCCAAATAAGCTACCCATCTGCATTCAGCCAGCTGATC	7	0.17500000000000002	No Hit
GAACATTGCCACTGCTCAGAATGGCGTCAAAGTAGTGAAGTGGATTCACA	7	0.17500000000000002	No Hit
GTTCATAGTCTCCTGCAGACCCACGAGGGCACTGTGCCTTTGCTGAGCTT	7	0.17500000000000002	No Hit
GTGCAATTCTTTAAGGCTTCTAAGCAGTGATAAAGTTGCTGTTAGGAGTG	7	0.17500000000000002	No Hit
GTTTACAGGAGCTGTGCCGAATGGAACCAAAGTCTGGCAATAGAATCCGT	7	0.17500000000000002	No Hit
GTCTAAACAAGCCAGCAAGAAATAGTATAATTGATGCTGCTAAAATCTGG	7	0.17500000000000002	No Hit
CTACCACTTACCCCTTTACCCTGCTCCCATGAGGGTACTCTCCCATTCAC	7	0.17500000000000002	No Hit
CTCTTTGTGAAATGCCTGAAGGTACTTTCTATGCAGGCCTGGCTGCACTT	7	0.17500000000000002	No Hit
CTGTGACCAAGCTCTAACTTCCAGTTTGAACACAGAATTAAATTAGGATG	7	0.17500000000000002	No Hit
CTTCGCCGCCCGAGCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCC	7	0.17500000000000002	No Hit
TTTCTTTATCCAGAGGCTTAGATAATGATGCTATGATGTATGTACATGGA	7	0.17500000000000002	No Hit
CCAACACTGACCCTTGGCTTCTTCGTTCTAAAAGCCCTGTGAGAAATCCT	7	0.17500000000000002	No Hit
GACATTGCCTCTTAGTTCCCAAACGAAAGAGTACATGGAGAACAAGAAAG	7	0.17500000000000002	No Hit
GTGGAAAGCCCAGCAAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGC	7	0.17500000000000002	No Hit
GTCTCCTGCAGACCCACGAGGGCACTGTGCCTTTGCTGAGCTTTCCAGAT	7	0.17500000000000002	No Hit
AGTGTGAGTTGCCTCTTTTGCAGACGTCCTAAGGCTAAGCTTGCCTGCTC	7	0.17500000000000002	No Hit
CCTGTGAATATGGTTTCATGACTTTGACTGAGCTACTGAAGAGTCTGCCT	7	0.17500000000000002	No Hit
GAGATCCCAGACACAACTATTTGCTTGTCCCAACAAGATGATGACATGGT	7	0.17500000000000002	No Hit
CTCTTACAAGGTTCCTTTTGTGGTTCTCTCTTTGAAAGTGTTCGCACCTC	7	0.17500000000000002	No Hit
GCCCAGCAAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGCCAACTTT	7	0.17500000000000002	No Hit
GGACACTGCCTTGTGGAATGGCCTTCTCTTCTGGGTGCCTGAGGTATCCA	7	0.17500000000000002	No Hit
CCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCTTCTGCTCCTCCCAAA	7	0.17500000000000002	No Hit
GTCATAAGAGAATTGTAGTGTTAAAAAATGACATGAAAAACCGGCTAAGT	7	0.17500000000000002	No Hit
CCTGGCCACACTGCCTAACAGTGGCCCAACCGCTTCAGTGCCCACACTGA	7	0.17500000000000002	No Hit
GACTGTCTTCCAAATAAGCTACCCATCTGCATTCAGCCAGCTGATCGCCT	7	0.17500000000000002	No Hit
GCAAGAAAGGCCTGCTAGCCCGCGCCTCCCTGGGATCTTCGCCGCCCGAG	7	0.17500000000000002	No Hit
AGTTATTTGAAAGAACTCCATTAAGGAATCTGTATTATATTTACCAAATT	6	0.15	No Hit
CTTCCACTAGTCAGATGACTTCTTTCGAGGGAAATGTTTATATTATTTTT	6	0.15	No Hit
TGTAATTTGAAGGCCAAGGTTGATCCTTGTTCTTGTGATTTAAAAATTGG	6	0.15	No Hit
GTCTCAGCTTTGACACAGAAGCTCTGCCATGTTGTAAAGGTTGCTGATAT	6	0.15	No Hit
TGAGAAGGGTGTGAACCTGTCGGGGGGCCAGAAGCAGCGTGTGAGCCTGG	6	0.15	No Hit
ACCAGGAATTTGGGTGAGGAATGGTTGTCTAAAGGTGCCACTGAGTCTGT	6	0.15	No Hit
GAATATTACACACATCCTCTTAAAAGTTCAAGATTCTTTAGGAAAGCAAT	6	0.15	No Hit
AGCTTACACCTCACTTGTGCACCAACTCTCTCCACCTAAATGTGGCACCT	6	0.15	No Hit
AAAGGTTGCTGATATGGAGTCTGGCAAACAAATCCAACTGATAACCCGGA	6	0.15	No Hit
AGTGGATTCACAACAAGCCCCCACCTCCCAACACTGACCCTTGGCTTCTT	6	0.15	No Hit
GTTATTTCATGCTTCCCTTCTCTGTCTGTCTCCGTTTGATAAGGGTAAGG	6	0.15	No Hit
CTGATTAGCAAGGATGCAGTGGAAAGCCCAGCAAAAAAGCAGCCCAAAAA	6	0.15	No Hit
CATTTGTTTAAGCAAATGTATTTGTGGTTGTTCAAGAAGCCAAACATGTC	6	0.15	No Hit
GTATCACGAATCTGTGTGCAAGCAGCACTGCTCGAACAAGGACTTCAGCG	6	0.15	No Hit
GAATGGTTGTCTAAAGGTGCCACTGAGTCTGTTCTATGGGATAGACAAAG	6	0.15	No Hit
CCAAAATAACGATGCTAAGCCGTGGCTTTGGAAATTCTCTGGTTGCTTTT	6	0.15	No Hit
GGATGTACCCAGTTAGATTTATCTGTTGAAGAGACAAAAGTTATCACCAG	6	0.15	No Hit
TGTGAGTTGCCTCTTTTGCAGACGTCCTAAGGCTAAGCTTGCCTGCTCAG	6	0.15	No Hit
ATATGGTTTCATGACTTTGACTGAGCTACTGAAGAGTCTGCCTTCTTTAG	6	0.15	No Hit
GCCCAGTTCTCTTTGAAGAGTTGGAGTATCACGAATCTGTGTGCAAGCAG	6	0.15	No Hit
TAAAGTTGCTGTTAGGAGTGTGAGTTGCCTCTTTTGCAGACGTCCTAAGG	6	0.15	No Hit
AGCTGCTCTAGAAATGTTCTGCAGGCTTGCCACTCTTTGTGAAATGCCTG	6	0.15	No Hit
CGCTCTTCCGATCTGTAGGCGCTCTGCCACTGAGCTAAATCCCCAGCCCC	6	0.15	No Hit
GAGAAACACTGCAGCCCAAAACTTATGGCTACAGTAGTGTGGAGGAGCTA	6	0.15	No Hit
ATCTGCTTCTGCTCCTCCCAAACCCTCCTCAGAAGCCCCCGGGCTACTGA	6	0.15	No Hit
TCTGTAATTTGAAGGCCAAGGTTGATCCTTGTTCTTGTGATTTAAAAATT	6	0.15	No Hit
GGAAAGCCCAGCAAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGCCA	6	0.15	No Hit
AGAGAATTGTAGTGTTAAAAAATGACATGAAAAACCGGCTAAGTTCATTT	6	0.15	No Hit
CCGGGCTACTGATTAGCAAGGATGCAGTGGAAAGCCCAGCAAAAAAGCAG	6	0.15	No Hit
ACCAAAGAGTGCACACAGAGGGACCAATGGCTCCAACTGCATGTGTAGTA	6	0.15	No Hit
CCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCTTCTGCTCCTC	6	0.15	No Hit
AAGCATAGATGGGACTATGGACTTTGAAAACACTAGGTTTCACTAAAGTT	6	0.15	No Hit
ATGGATTCTTATTTCATGGCCAGCATTTTGATAAAAATGTCACAAAATTA	6	0.15	No Hit
CCTTGTTCTTGTGATTTAAAAATTGGCTGTAAATCTGGGAAGCTCTCAAT	6	0.15	No Hit
GTTCTGACCCAGACTGCACCACGGTTCATGTGCTGTGTGCTCCTGGCAGG	6	0.15	No Hit
CTTCTCTTCTGGGTGCCTGAGGTATCCACTTATGAGTGGCCTCTCACCGC	6	0.15	No Hit
ACGTCCTAAGGCTAAGCTTGCCTGCTCAGACTAAAGGTTTGCAAGCATAG	6	0.15	No Hit
CATCTCTTCATCCCAGATAGGTCACAAATACAGACTGCAGAAATGATTCC	6	0.15	No Hit
GATAGCACTTCCTCTTTAAGGCCACATTGCACTTCCTCTGCAATCTTAGT	6	0.15	No Hit
CCTCTCACCGCTGTTGGAGAGACCTGACATTTACAATGGATTGTATCTGT	6	0.15	No Hit
ACTAAAGGTTTGCAAGCATAGATGGGACTATGGACTTTGAAAACACTAGG	6	0.15	No Hit
GAAAGTTCCTGGCTTCTAGGTGACTTATTGGTGTGCCTTGTTTTTTATGT	6	0.15	No Hit
GAGACACTTCACAGAAGCAGTCAAAAAAAAGAGAACCTCAGTTCCCAGAG	6	0.15	No Hit
CTGTATTATATTTACCAAATTTTTCTAGACTATTGTGAATTGTGGTGGTT	6	0.15	No Hit
CCTCACAGAGATTGGTGAGAAGGGTGTGAACCTGTCGGGGGGCCAGAAGC	6	0.15	No Hit
CCCAGTTAGATTTATCTGTTGAAGAGACAAAAGTTATCACCAGAATCCCC	6	0.15	No Hit
AAGGGCTGGTCGGCAAGAAAGGCCTGCTAGCCCGCGCCTCCCTGGGATCT	6	0.15	No Hit
GGAAGAGGAGGCAACAGGGTTGCTGCACCCATGAGAAGGTATGAAGGAAC	6	0.15	No Hit
TGGATATTCCAAGCTGATTGAGCTACTAGAAGCCGTGCCTCATGTGTTGC	6	0.15	No Hit
GCCTGAAGGTACTTTCTATGCAGGCCTGGCTGCACTTCTTTTCTGAGGCC	6	0.15	No Hit
CACACTGGGGCATGATTGAGTGGCAAGAGAGCCATTTTCCGGTTGACAGG	6	0.15	No Hit
GAAGCCGTGCCTCATGTGTTGCAGATTCTTGGAATGGGCTCCAAGCGTCT	6	0.15	No Hit
GAGCTACTGGGAGCCATCCCACAGGTGGTCTGGATAAAAGGACATGGTCA	6	0.15	No Hit
ATGCTATGATGTATGTACATGGAGATAATGAGACACTTTTTCTGGGTGGA	6	0.15	No Hit
GCTTACACCTCACTTGTGCACCAACTCTCTCCACCTAAATGTGGCACCTC	5	0.125	No Hit
CTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCCCATTTTACCATGGA	5	0.125	No Hit
CAAACATGTCCTTAAACCTGTGGGTCACACACTGGGGCATGATTGAGTGG	5	0.125	No Hit
GCCAGGTCTATGCAGTTAAAATCACTCACAGCCACAAAGTCTTCCTGCCA	5	0.125	No Hit
GCCGGGCGAACGCCAGAGTATGGCGCCTCTGTTGGAGTACGAGAGGCAGC	5	0.125	No Hit
GTTGCAAACTTCTTATGTTCCTTCAGTCCTTCCCCTAACTCTACCATTGG	5	0.125	No Hit
AGATAATGAGACACTTTTTCTGGGTGGAAGGATGTACCCAGTTAGATTTA	5	0.125	No Hit
TGTCAGTTCAGTCTCAGCTTTGACACAGAAGCTCTGCCATGTTGTAAAGG	5	0.125	No Hit
AACTTTTTCTTACTTTATTTCTTCCAGTTATTTGAAAGAACTCCATTAAG	5	0.125	No Hit
ACGAATCTGTGTGCAAGCAGCACTGCTCGAACAAGGACTTCAGCGAACTT	5	0.125	No Hit
TGGGACTATGGACTTTGAAAACACTAGGTTTCACTAAAGTTACTTGGAGT	5	0.125	No Hit
CCCGAGCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCCCATTTTAC	5	0.125	No Hit
CGTCCTAAGGCTAAGCTTGCCTGCTCAGACTAAAGGTTTGCAAGCATAGA	5	0.125	No Hit
GTCATGCCCATATGATATTCCAGAAACTCTTATGTCAGATCTATGGAAAA	5	0.125	No Hit
AGCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCCCATTTTACCATG	5	0.125	No Hit
CTCACCCAGTAGCCCAGCATCTGCTTCTGCTCCTCCCAAACCCTCCTCAG	5	0.125	No Hit
AGTGCTTCAAATGGTGTGGGTTCAATATTATTTCCACTAAGAAGAAACCA	5	0.125	No Hit
GTCACACACTGGGGCATGATTGAGTGGCAAGAGAGCCATTTTCCGGTTGA	5	0.125	No Hit
AGGGCACTGTGCCTTTGCTGAGCTTTCCAGATTGCTATGCTGCGGAGTTT	5	0.125	No Hit
AGCCTCCACAGATACAAAATTGGCAGCAAGAAGATACTGGTGTCACTCTC	5	0.125	No Hit
CAGTAAAGAAGGGGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCA	5	0.125	No Hit
CAGCCCACTGATCTCATTCAGTTTGAGGAACACCCTCAGGAGCCTTTGGG	5	0.125	No Hit
CTAGAAATGTTCTGCAGGCTTGCCACTCTTTGTGAAATGCCTGAAGGTAC	5	0.125	No Hit
CATGAATATTACACACATCCTCTTAAAAGTTCAAGATTCTTTAGGAAAGC	5	0.125	No Hit
AAAATATAACAATAAAATATAATAAGATAAAACAAAGACTATCACATTGA	5	0.125	No Hit
GGGAGGTCTTACTCCTCAATGTCTTGAGTCTCCAGCGCTGCTGCTCTAGATGCCCCCCTGTGCTGTACCTTGTAG	5	0.125	No Hit
TGTCCCTGTATTTCCTTTAGACAAGAGCAGTTATGGATTAATATTTTTGA	5	0.125	No Hit
ATCTTCGCCGCCCGAGCTGGACATCATGCCACAGGAAGAGTGAGCTCTTG	5	0.125	No Hit
TGTGAATTGTGGTGGTTGGGAAGAAATAAGCAGAGATCGTTCAGCAGGAA	5	0.125	No Hit
CAGTCTTCCACTAGTCAGATGACTTCTTTCGAGGGAAATGTTTATATTAT	5	0.125	No Hit
TGTAACTCCTCACCCAGTAGCCCAGCATCTGCTTCTGCTCCTCCCAAACC	5	0.125	No Hit
AGAAATTCCAATACCAGTAAAGAAGGGGAACCTGTCCTGTAACTCCTCAC	5	0.125	No Hit
GGGGGCCAGAAGCAGCGTGTGAGCCTGGCTCGGGCTGTGTATTGTAACTC	5	0.125	No Hit
GCAGGAAGTGGATTGAGTCCCATTGAGCAGCCTCCATCATTTGTTTAAGC	5	0.125	No Hit
GATTTTTTTTACCAGTTGAATTTTTTTAATCAATATCTTTTAAATGAAGC	5	0.125	No Hit
TCCGTCAGAGCCTGCGAAGGTTTGCAAATACGCACACTGCTCCAGCTACT	5	0.125	No Hit
CAAACGAAAGAGTACATGGAGAACAAGAAAGCTGCTGTGGAGTTAAAGGA	5	0.125	No Hit
GCAGTGGAAAGCCCAGCAAAAAAGCAGCCCAAAAATAGAGTCAAATTGGC	5	0.125	No Hit
CGCCGCCCGAGCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCCCAT	5	0.125	No Hit
GGGAGAACTTCAAGTTCTCTGCTCTTGCCCTCAAGGCGTTTTTTCCATCT	5	0.125	No Hit
AACTATGTCTGTGGTTAACCAATGTGGCCTCATCCCATTTTTTAAAAAAG	5	0.125	No Hit
GGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCTTCTGCT	5	0.125	No Hit
CTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTTTTCCTGAGGTC	5	0.125	No Hit
CCACACTGCCTAACAGTGGCCCAACCGCTTCAGTGCCCACACTGAAAAAC	5	0.125	No Hit
GAAGCCTGTTGTTGAATGTTCTTCCAAGTACTTACACATAGGGGCATATA	5	0.125	No Hit
CTTGCATATTATGGATTTACCAAGTTGCTGGAACTTTTGGAAGCCATACC	5	0.125	No Hit
GTTTGCAAGCATAGATGGGACTATGGACTTTGAAAACACTAGGTTTCACT	5	0.125	No Hit
GCTAAGCTTGCCTGCTCAGACTAAAGGTTTGCAAGCATAGATGGGACTAT	5	0.125	No Hit
AGCAGCACTGCTCGAACAAGGACTTCAGCGAACTTGTATTTGATCCAGAC	5	0.125	No Hit
GTCCAATCACTATTTCTTTATCCAGAGGCTTAGATAATGATGCTATGATG	5	0.125	No Hit
TAAGCAGAGATCGTTCAGCAGGAAGTGGATTGAGTCCCATTGAGCAGCCT	5	0.125	No Hit
CACAGGCTTATCATTGGTGTTTCTCAAAGGACTGGGATGTCACGGAATAC	5	0.125	No Hit
GAAAAATATTTAAAGATTCAGAGCATGGATGGGATCAAAGCTGAGTCTGA	5	0.125	No Hit
CAGGTGTCCGGCCAGGTGCTGATCTGTGCGAGCGATGACCGCACATGCTG	5	0.125	No Hit
CTGACCCTTGGCTTCTTCGTTCTAAAAGCCCTGTGAGAAATCCTCAGCTG	5	0.125	No Hit
CTGGGATCTTCGCCGCCCGAGCTGGACATCATGCCACAGGAAGAGTGAGC	5	0.125	No Hit
TCACCCCAGCATGGATGTTGACAAACACATACTAGATCCATACTTGCAGG	5	0.125	No Hit
AGAAGGCCTGCTAGCCCGCGCCTCCCTGGGATCTTCGCCGCCCGAGCTGG	5	0.125	No Hit
AGCATACAGAATACCTTCGTGTACCAAAACACTAGGATGTAGGGATGAAG	5	0.125	No Hit
CCAACTCCTCCCTCCTCTCTGCCCTGGTATTCTTCTACCCTGAGATGTCC	5	0.125	No Hit
GTGCCTGAGGTATCCACTTATGAGTGGCCTCTCACCGCTGTTGGAGAGAC	5	0.125	No Hit
CATCCCACAGGTGGTCTGGATAAAAGGACATGGTCATAAGAGAATTGTAG	5	0.125	No Hit
AGGAGCTGGTGGCTGAGGGTGGAAGAATGAAAGAAAAGAAAGATGAAAGA	5	0.125	No Hit
CGTGTCCCAGATGTCAAACTGAGCAAAAAGACCAAAACATCGGAGAAGAT	5	0.125	No Hit
CGGCAAGAAAGGCCTGCTAGCCCGCGCCTCCCTGGGATCTTCGCCGCCCG	5	0.125	No Hit
CTGAAGGTACTTTCTATGCAGGCCTGGCTGCACTTCTTTTCTGAGGCCTG	5	0.125	No Hit
GCCGAGGAGGCATCCTCGTGTACAGAGCACACAGGATCATTGAGTCTTGC	5	0.125	No Hit
GCCTATACTAAATATGTAGGAGAAACACTGCAGCCCAAAACTTATGGCTA	5	0.125	No Hit
CTTGATTGATATAATATCAGAGATCCCAGACACAACTATTTGCTTGTCCC	5	0.125	No Hit
GGGAGCCTGGCCACACTGCCTAACAGTGGCCCAACCGCTTCAGTGCCCAC	5	0.125	No Hit
CTTAGATAATGATGCTATGATGTATGTACATGGAGATAATGAGACACTTT	5	0.125	No Hit
CTCCGTCAGAGCCTGCGAAGGTTTGCAAATACGCACACTGCTCCAGCTAC	5	0.125	No Hit
AGACTATGGATATTCCAAGCTGATTGAGCTACTAGAAGCCGTGCCTCATG	5	0.125	No Hit
CTCCAAGACTATGATGTCAGTTCAGTCTCAGCTTTGACACAGAAGCTCTG	5	0.125	No Hit
TTGCGGACACTGCCTTGTGGAATGGCCTTCTCTTCTGGGTGCCTGAGGTA	5	0.125	No Hit
AGAGCAGAGAGAACCTGTTGGCACACAGTCTTCCACTAGTCAGATGACTT	5	0.125	No Hit
GCCTGCGAAGGTTTGCAAATACGCACACTGCTCCAGCTACTGTGGTTCTT	5	0.125	No Hit
GTGACATCAGTAAAGAGAACAAGGAAGTCATTCAAGAGCTGAATAATTGT	5	0.125	No Hit
ATTTGAAGAGTTCATTTCCGATCTACCCCCCAGGTTACCACTGAAGATTC	5	0.125	No Hit
CATACCTGAGATTTTACAAGTGTTGGAATGTGGAGAAGAGAAAATCCTGA	5	0.125	No Hit
ATCCACTTATGAGTGGCCTCTCACCGCTGTTGGAGAGACCTGACATTTAC	5	0.125	No Hit
ATTGTAAATCAGCTGAGTGGTGAAGTGCTTCAAATGGTGTGGGTTCAATA	5	0.125	No Hit
GCCTCCATCATTTGTTTAAGCAAATGTATTTGTGGTTGTTCAAGAAGCCA	5	0.125	No Hit
GTGAAGCGCTTTACTCAGGATTTACTGAAACTTCTCAAGTCCCAGGCCAG	5	0.125	No Hit
GGCAAATCCATTCATAGAGCAGAGAGAACCTGTTGGCACACAGTCTTCCA	5	0.125	No Hit
GCTCAGACTAAAGGTTTGCAAGCATAGATGGGACTATGGACTTTGAAAAC	5	0.125	No Hit
CTGAAGAGTCTGCCTTCTTTAGTAGAGGTTTTTATGAATGATAAGGCAGA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.0	0.0	0.0	0.0	0.0
78-79	0.0	0.0	0.0	0.0	0.0
80-81	0.0	0.0	0.0	0.0	0.0
82-83	0.0	0.0	0.0	0.0	0.0
84-85	0.0	0.0	0.0	0.0	0.0
86-87	0.0	0.0	0.0	0.0	0.0
88-89	0.0	0.0	0.0	0.0	0.0
90-91	0.0	0.0	0.0	0.0	0.0
92-93	0.0	0.0	0.0	0.0	0.0
94-95	0.0	0.0	0.0	0.0	0.0
96-97	0.0	0.0	0.0	0.0	0.0
98-99	0.0	0.0	0.0	0.0	0.0
100-101	0.0	0.0	0.0	0.0	0.0
102-103	0.0	0.0	0.0	0.0	0.0
104-105	0.0	0.0	0.0	0.0	0.0
106-107	0.0	0.0	0.0	0.0	0.0
108-109	0.0	0.0	0.0	0.0	0.0
110-111	0.0	0.0	0.0	0.0	0.0
112-113	0.0	0.0	0.0	0.0	0.0
114-115	0.0	0.0	0.0	0.0	0.0
116-117	0.0	0.0	0.0	0.0	0.0
118-119	0.0	0.0	0.0	0.0	0.0
120-121	0.0	0.0	0.0	0.0	0.0
122-123	0.0	0.0	0.0	0.0	0.0
124-125	0.0	0.0	0.0	0.0	0.0
126-127	0.0	0.0	0.0	0.0	0.0
128-129	0.0	0.0	0.0	0.0	0.0
130-131	0.0	0.0	0.0	0.0	0.0
132-133	0.0	0.0	0.0	0.0	0.0
134-135	0.0	0.0	0.0	0.0	0.0
136-137	0.0	0.0125	0.0	0.0	0.0
138-139	0.0	0.025	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTGGAT	20	6.128537E-4	95.05313	1
GTGGATT	20	6.128537E-4	95.05313	2
TTTTTTT	70	0.0010731714	17.881834	130-134
>>END_MODULE
SRR26281810 read2 length is 43-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR26281810_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	43-151
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.2635	37.0	37.0	37.0	37.0	37.0
2	36.3445	37.0	37.0	37.0	37.0	37.0
3	36.4555	37.0	37.0	37.0	37.0	37.0
4	36.4055	37.0	37.0	37.0	37.0	37.0
5	36.376	37.0	37.0	37.0	37.0	37.0
6	36.3165	37.0	37.0	37.0	37.0	37.0
7	36.215	37.0	37.0	37.0	37.0	37.0
8	36.203	37.0	37.0	37.0	37.0	37.0
9	36.2485	37.0	37.0	37.0	37.0	37.0
10-14	36.3109	37.0	37.0	37.0	37.0	37.0
15-19	36.3094	37.0	37.0	37.0	37.0	37.0
20-24	36.3283	37.0	37.0	37.0	37.0	37.0
25-29	36.2643	37.0	37.0	37.0	37.0	37.0
30-34	36.1794	37.0	37.0	37.0	37.0	37.0
35-39	36.1306	37.0	37.0	37.0	37.0	37.0
40-44	36.200961915478864	37.0	37.0	37.0	37.0	37.0
45-49	36.1488713501256	37.0	37.0	37.0	37.0	37.0
50-54	36.13626302851016	37.0	37.0	37.0	37.0	37.0
55-59	36.09376885501531	37.0	37.0	37.0	37.0	37.0
60-64	36.04947078334576	37.0	37.0	37.0	37.0	37.0
65-69	35.99029249586697	37.0	37.0	37.0	37.0	37.0
70-74	36.06038004779727	37.0	37.0	37.0	37.0	37.0
75-79	35.97405602801793	37.0	37.0	37.0	37.0	37.0
80-84	35.94550025262588	37.0	37.0	37.0	37.0	37.0
85-89	35.98397641250866	37.0	37.0	37.0	37.0	37.0
90-94	35.882273380091	37.0	37.0	37.0	37.0	37.0
95-99	35.86898340799753	37.0	37.0	37.0	37.0	37.0
100-104	35.909722571084174	37.0	37.0	37.0	37.0	37.0
105-109	35.8269760158242	37.0	37.0	37.0	37.0	37.0
110-114	35.75875984269746	37.0	37.0	37.0	37.0	37.0
115-119	35.893142211388465	37.0	37.0	37.0	37.0	37.0
120-124	35.75761755249185	37.0	37.0	37.0	37.0	37.0
125-129	35.63533718372636	37.0	37.0	37.0	37.0	37.0
130-134	35.5830629374081	37.0	37.0	37.0	37.0	37.0
135-139	35.4583556458886	37.0	37.0	37.0	37.0	37.0
140-144	35.334671825333494	37.0	37.0	37.0	32.2	37.0
145-149	35.15822676856153	37.0	37.0	37.0	27.4	37.0
150-151	35.5960947270363	37.0	37.0	37.0	31.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	0.0
23	2.0
24	4.0
25	7.0
26	8.0
27	21.0
28	16.0
29	29.0
30	39.0
31	61.0
32	67.0
33	100.0
34	152.0
35	424.0
36	2598.0
37	471.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.175	9.175	18.275	40.375
2	21.6	7.375	32.975	38.05
3	20.825	11.75	22.6	44.824999999999996
4	23.599999999999998	16.7	19.925	39.775
5	24.75	22.7	26.025	26.525
6	26.450000000000003	27.075	23.025000000000002	23.45
7	16.75	30.15	36.7	16.400000000000002
8	25.374999999999996	24.775	31.45	18.4
9	20.4	24.05	33.074999999999996	22.475
10-14	21.525	29.765000000000004	28.005000000000003	20.705000000000002
15-19	21.335	28.349999999999998	28.93	21.385
20-24	21.0	26.419999999999998	31.385	21.195
25-29	23.365	26.755000000000003	28.73	21.15
30-34	21.13	28.694999999999997	27.229999999999997	22.945
35-39	22.05	26.44	29.925	21.584999999999997
40-44	22.266113305665282	28.07140357017851	29.43647182359118	20.226011300565027
45-49	22.011508631473607	26.810107580685518	27.805854390793094	23.372529397047785
50-54	23.21097701437228	28.12359156692874	25.719865791977565	22.945565626721418
55-59	20.862552594670404	25.806451612903224	29.127429372871166	24.2035664195552
60-64	22.03143043631069	27.78028819601346	27.649746447758194	22.53853491991766
65-69	21.93772322551436	27.521505105890636	29.16645706524473	21.37431460335027
70-74	22.991465939504117	26.011210422663233	26.9959097106499	24.00141392718275
75-79	22.061436912922062	25.991368367605993	30.022848438690026	21.924346280781926
80-84	20.82120422858894	27.55221898779429	28.338695674378222	23.287881109238548
85-89	22.05334987593052	29.151157981803145	28.03970223325062	20.755789909015714
90-94	21.779847232395102	28.57068117610129	28.09459035262111	21.554881238882494
95-99	24.010046491743708	29.0332923635975	25.233794688184684	21.722866456474108
100-104	23.102328397644083	25.43623052788022	31.094842296471626	20.366598778004075
105-109	23.203076401063168	27.404852117853306	29.03919018266131	20.35288129842221
110-114	22.192103712433706	26.2286387743076	28.196817913965823	23.38243959929287
115-119	23.0175395256917	26.61808300395257	26.513092885375496	23.851284584980238
120-124	20.783404200489628	26.691148047931968	27.74771292359232	24.777734827986084
125-129	22.108751521709724	24.881644799134317	29.19653726498039	23.81306641417557
130-134	21.90489997100609	27.24702812409394	29.40707451435199	21.440997390547984
135-139	18.472168905950095	27.178502879078696	28.629558541266793	25.719769673704413
140-144	21.256155647049326	28.36037781545168	28.15855332203116	22.22491321546783
145-149	24.466268146883007	28.445772843723315	24.73953885567891	22.348420153714773
150-151	24.622222222222224	12.681481481481482	33.68888888888889	29.00740740740741
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.5
22	3.5
23	2.0
24	0.0
25	2.0
26	4.5
27	7.5
28	12.0
29	19.5
30	20.5
31	32.0
32	54.0
33	44.5
34	51.5
35	74.5
36	89.0
37	112.0
38	113.5
39	109.5
40	157.5
41	189.5
42	199.0
43	241.0
44	281.5
45	288.0
46	244.5
47	204.5
48	197.0
49	174.5
50	168.5
51	175.5
52	153.0
53	131.5
54	106.0
55	81.0
56	91.5
57	79.0
58	48.0
59	30.5
60	11.5
61	8.5
62	5.0
63	3.5
64	0.5
65	1.5
66	4.0
67	5.5
68	5.0
69	4.5
70	9.0
71	7.5
72	1.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
40-44	2.0
45-49	3.0
50-54	2.0
55-59	8.0
60-64	8.0
65-69	9.0
70-74	16.0
75-79	25.0
80-84	35.0
85-89	48.0
90-94	59.0
95-99	114.0
100-104	84.0
105-109	132.0
110-114	160.0
115-119	135.0
120-124	143.0
125-129	182.0
130-134	169.0
135-139	138.0
140-144	126.0
145-149	376.0
150-152	2026.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	41.70818505338078	14.649999999999999
2	19.572953736654807	13.750000000000002
3	12.882562277580071	13.575000000000001
4	8.96797153024911	12.6
5	6.334519572953737	11.125
6	3.9145907473309607	8.25
7	2.0640569395017794	5.075
8	1.1387900355871887	3.2
9	0.8540925266903915	2.7
>10	2.491103202846975	13.775
>50	0.0711743772241993	1.3
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTCCCCAGATCACACACCCATTCACTGTACACAACTCTTCTTTGTTGCCC	52	1.3	No Hit
CTCGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTTCTC	36	0.8999999999999999	No Hit
CCCCAGATCACACACCCATTCACTGTACACAACTCTTCTTTGTTGCCCAC	29	0.7250000000000001	No Hit
CCCAGATCACACACCCATTCACTGTACACAACTCTTCTTTGTTGCCCACA	28	0.7000000000000001	No Hit
CCCCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGT	28	0.7000000000000001	No Hit
CCTCGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTTCT	27	0.675	No Hit
GTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACCTCC	25	0.625	No Hit
CCCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGTC	23	0.575	No Hit
CCTCTCAATACACAATATGTCATTCAAATGCTGACTTAAATGGATGACAT	21	0.525	No Hit
CTCCCAAGTCTCATCAAGAGCAAACAGTGTAGGATGGGAGGATGGGAAAG	20	0.5	No Hit
CCGGCCAAAGTGGTGGTGGTAAGAGGGAATGAACTTGTTAAAGGGCATTC	18	0.44999999999999996	No Hit
GCCAGATTTTAGCAGCATCAATTATACTATTTCTTGCTGGCTTGTTTAGA	17	0.42500000000000004	No Hit
ACCCGGCACTGCTTTCCAAAATGGTGATGGTACAAAGGGATGAAATTACT	16	0.4	No Hit
GTTCTTTAGTAAATCAATGACTTCTCTGCTGAACTGAATCAGCTGAGGAT	15	0.375	No Hit
CTCTCAATACACAATATGTCATTCAAATGCTGACTTAAATGGATGACATC	14	0.35000000000000003	No Hit
GCTGCTTTTTTGCTGGGCTTTCCACTGCATCCTTGCTAATCAGTAGCCCG	14	0.35000000000000003	No Hit
TCCCAAGTCTCATCAAGAGCAAACAGTGTAGGATGGGAGGATGGGAAAGG	14	0.35000000000000003	No Hit
CTGGCCTGGGACTTGAGAAGTTTCAGTAAATCCTGAGTAAAGCGCTTCAC	13	0.325	No Hit
GGCAGGTCATAGTGCTGAGAACTGGGAATCACTGGCTTCATTTAAAAGAT	13	0.325	No Hit
CCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACCTCCAG	12	0.3	No Hit
CTCCTCTGATCCAAAAGTCTTTCCTGAGTCCTCCATGGTAAAATGGGCAA	12	0.3	No Hit
GTCCGCAAAGGCTAGCTTTTCCCAAATGCCAACATCAGCTACCATAATAA	11	0.27499999999999997	No Hit
CCCCAGTGTGTGACCCACAGGTTTAAGGACATGTTTGGCTTCTTGAACAA	11	0.27499999999999997	No Hit
GCCGGTTTTTCATGTCATTTTTTAACACTACAATTCTCTTATGACCATGT	11	0.27499999999999997	No Hit
GTCTTGTTCTTCAGTAGGCCCATGGGACCCACCACCTTCTCAAAGATGTG	11	0.27499999999999997	No Hit
CCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGTCA	11	0.27499999999999997	No Hit
GTCCTCTAAAAAACTTCTCACGGATTCTCTGTACAACAGTTGTTGCTGAC	11	0.27499999999999997	No Hit
GCCATCTTTATTTGCCGGCAGGTTATAAACATAGAGTAGAGTGTGGCAAG	10	0.25	No Hit
CCGGCACTGCTTTCCAAAATGGTGATGGTACAAAGGGATGAAATTACTGA	10	0.25	No Hit
GGGGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAGAAT	10	0.25	No Hit
CTGGGGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAGA	10	0.25	No Hit
TGGCCATGAAATAAGAATCCATTTTCTGGCTTTCTAGAAAAAATAATATA	10	0.25	No Hit
ACCGTATTCCGTGACATCCCAGTCCTTTGAGAAACACCAATGATAAGCCT	10	0.25	No Hit
CCACAGTTATCAGAGAGGCGCCTGAGCCTGTTGCTGATGCTCTTGCCATC	10	0.25	No Hit
CCCAGATTTACAGCCAATTTTTAAATCACAAGAACAAGGATCAACCTTGG	10	0.25	No Hit
CACGGATTCTCTGTACAACAGTTGTTGCTGACCGGCCAGAGGGGACTGAG	10	0.25	No Hit
GGTCAGTAGACGCTTGGAGCCCATTCCAAGAATCTGCAACACATGAGGCA	9	0.22499999999999998	No Hit
TCCCTCGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTT	9	0.22499999999999998	No Hit
GCGAGGCGGTGTTCAAAAGGTTTGGAGACATACTCCTCGGAGACCAAGAC	9	0.22499999999999998	No Hit
TTCCCCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTG	9	0.22499999999999998	No Hit
CACCGTATTCCGTGACATCCCAGTCCTTTGAGAAACACCAATGATAAGCC	9	0.22499999999999998	No Hit
CTCACGGATTCTCTGTACAACAGTTGTTGCTGACCGGCCAGAGGGGACTG	9	0.22499999999999998	No Hit
TGCTTTTTTGCTGGGCTTTCCACTGCATCCTTGCTAATCAGTAGCCCGGG	9	0.22499999999999998	No Hit
CCGTGACATCCCAGTCCTTTGAGAAACACCAATGATAAGCCTGTGAGAAA	9	0.22499999999999998	No Hit
GGCAGTGTCCGCAAAGGCTAGCTTTTCCCAAATGCCAACATCAGCTACCA	9	0.22499999999999998	No Hit
GCCGGCAGGTTATAAACATAGAGTAGAGTGTGGCAAGGAATCTTCAGTGG	9	0.22499999999999998	No Hit
GCTGGTAGTGATAGGTATGAAGCAAAGACCTCACATTCTTTGCAAACAAG	9	0.22499999999999998	No Hit
GGGCTGGTGGCGCAGCAAGTCCACAACATCTTTGGAGAACTGCTTTGTCC	9	0.22499999999999998	No Hit
CCCAAACATGCTTCCAACAGTGAACAGTAACAGTAAAGCTAGACACTATT	8	0.2	No Hit
GCGGTGTTCAAAAGGTTTGGAGACATACTCCTCGGAGACCAAGACTGAGA	8	0.2	No Hit
CCCGGCACTGCTTTCCAAAATGGTGATGGTACAAAGGGATGAAATTACTG	8	0.2	No Hit
GCCTTAAAGAGGAAGTGCTATCAGGCCTCAGAAAAGAAGTGCAGCCAGGC	8	0.2	No Hit
CCCGGTTTCTGGTTTCAGCTTGCTGCAGCTGAGTCTCCAATTCAGCTTCA	8	0.2	No Hit
GGAAGCATGAAATAACTTTCTAGAAAGCTCTTAATTAAATGGAATCCCCA	8	0.2	No Hit
TCCCCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGG	8	0.2	No Hit
CTCCGGTCGGCGCCGAGCCCGCGGGCGCACACCACCAGCCCGTCGCTGTC	8	0.2	No Hit
CTCTCCAATAGTTTGTTTCTTCCTCCTCTGATCCAAAAGTCTTTCCTGAG	8	0.2	No Hit
CACCTCCAGGATCTGCCTGCTGACTGCAGGCTGGTCTTCATGATTGACAG	8	0.2	No Hit
CCAGCAACTGAACAGTGAGAGGCCGCAAAGACTTCCGGGTTATCAGTTGG	8	0.2	No Hit
GGCCAGGTCTCTCCAATAGTTTGTTTCTTCCTCCTCTGATCCAAAAGTCT	8	0.2	No Hit
GCTGACTTAAATGGATGACATCGAGACCATCTCTTTTCCTTAAACCTTAC	8	0.2	No Hit
GTGTGACCCACAGGTTTAAGGACATGTTTGGCTTCTTGAACAACCACAAA	8	0.2	No Hit
CTGTCATCATAAGGCAGTTACCCTTTTGGGAACGCAAAAGCTTAACAAAC	8	0.2	No Hit
CTGCCAATTTTGTATCTGTGGAGGCTGTTCACAGCACAGATTGCGTCATG	8	0.2	No Hit
TCACCGTATTCCGTGACATCCCAGTCCTTTGAGAAACACCAATGATAAGC	7	0.17500000000000002	No Hit
CTTCTGTGCAGGAGAGCTGGCAATGCTCTGGGAACTGAGGTTCTCTTTTT	7	0.17500000000000002	No Hit
CTGCTCAATGGGACTCAATCCACTTCCTGCTGAACGATCTCTGCTTATTT	7	0.17500000000000002	No Hit
TCAATACACAATATGTCATTCAAATGCTGACTTAAATGGATGACATCGAG	7	0.17500000000000002	No Hit
TGGCGCAGCAAGTCCACAACATCTTTGGAGAACTGCTTTGTCCTTTCTAT	7	0.17500000000000002	No Hit
CTCCAATAGTTTGTTTCTTCCTCCTCTGATCCAAAAGTCTTTCCTGAGTC	7	0.17500000000000002	No Hit
GTGAGGGTCAGTAGACGCTTGGAGCCCATTCCAAGAATCTGCAACACATG	7	0.17500000000000002	No Hit
GTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAGAATCAG	7	0.17500000000000002	No Hit
CGGATAGGAAACCATAAAAGATTCTGTGGGAAGAGACCTTCCCACAGATT	7	0.17500000000000002	No Hit
CCCCCCCAAAAAAGTGTTCAAATTTGACAGTTAAGAAAAACCTCTTGTCT	7	0.17500000000000002	No Hit
CCCGCTCTGCGCTGTCTTGGTTTATAAAGCGGAGAATAGCGCTGCAGCCT	7	0.17500000000000002	No Hit
GGGTAGCTTATTTGGAAGACAGTCCCCTCTCTCTTTTTCTTCTGTGCAGG	7	0.17500000000000002	No Hit
TCCTCTCAATACACAATATGTCATTCAAATGCTGACTTAAATGGATGACA	7	0.17500000000000002	No Hit
GGATAATCAACTGAGCAATCCTGTCACCTGAGAAGATGGAAAAAACGCCT	7	0.17500000000000002	No Hit
GGCTGGGTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGC	7	0.17500000000000002	No Hit
CTTTTTTGCTGGGCTTTCCACTGCATCCTTGCTAATCAGTAGCCCGGGGG	7	0.17500000000000002	No Hit
TGAGAACTGGGAATCACTGGCTTCATTTAAAAGATATTGATTAAAAAAAT	7	0.17500000000000002	No Hit
CAGCAATGGTTAGTCACTCCCCAGATCACACACCCATTCACTGTACACAA	7	0.17500000000000002	No Hit
GCCTGAGATCACTGAGTTCTAATGCAAAATTAACATCAGTTGACACAAGA	7	0.17500000000000002	No Hit
AGGGCTGCTTTTTTGCTGGGCTTTCCACTGCATCCTTGCTAATCAGTAGC	7	0.17500000000000002	No Hit
TTGCTGAAGAGAATGAATATCAGGAAGGGGAACTGCTGGGAAAAATTTAG	7	0.17500000000000002	No Hit
CCGGCAGGTTATAAACATAGAGTAGAATGTGGCAAGGAATCTTCAGTGGT	7	0.17500000000000002	No Hit
GGCAAATTCATGAAGAAAAATCTGCTGGTAGTGATAGGTATGAAGCAAAG	7	0.17500000000000002	No Hit
CTCTATTTTTGGGCTGCTTTTTTGCTGGGCTTTCCACTGCATCCTTGCTA	7	0.17500000000000002	No Hit
CACTGTACACAACTCTTCTTTGTTGCCCACAGAACCTGACCTTTGAGATG	7	0.17500000000000002	No Hit
CTGGACATCTCAGGGTAGAAGAATACCAGGGCAGAGAGGAGGGAGGAGTT	7	0.17500000000000002	No Hit
CTCTGATAATAGGTCCCGGTTTCTGGTTTCAGCTTGCTGCAGCTGAGTCT	7	0.17500000000000002	No Hit
TCTCTGGGAACTGAGGTTCTCTTTTTTTTGACTGCTTCTGTGAAGTGTCT	7	0.17500000000000002	No Hit
GGGGCTTGTTGTGAATCCACTTCACTACTTTGACGCCATTCTGAGCAGTG	7	0.17500000000000002	No Hit
TTCCCAACATGTGCATCCACAGCCGAGAGGGGGTCGTCCAAGAGGTAGAT	6	0.15	No Hit
GTCCCAGTGTGTGACCCACAGGTTTAAGGACATGTTTGGCTTCTTGAACA	6	0.15	No Hit
CACCACTCAGCTGATTTACAATTTATGCATTTAATGCATAGTTTAAAAAA	6	0.15	No Hit
GCTCTCTGAATGGATTTGCCCTTTTTGCCCAACAGATACAATCCATTGTA	6	0.15	No Hit
GACAGCCTGTAGTCCACATTGCTGACCTGGATGTCAACACCATTTGCAAA	6	0.15	No Hit
AGAGACTATTCCCAGAGGTTAGTCATGGCCTCCGGTTAAGGGATGGAGCC	6	0.15	No Hit
CTCTTCCTGTGGACACTGCTTCTTGACTAGGGAGGCCTCTTCTAGAAGCT	6	0.15	No Hit
GCAGCATAGCAATCTGGAAAGCTCAGCAAAGGCACAGTGCCCTCGTGGGT	6	0.15	No Hit
CTCCAGGATCTGCCTGCTGACTGCAGGCTGGTCTTCATGATTGACAGGGG	6	0.15	No Hit
TATCTAAGCCTCTGGATAAAGAAATAGTGATTGGACTGGCCTCTTGGGCA	6	0.15	No Hit
TTCCCAAATGCCAACATCAGCTACCATAATAATAAAAACCCAAACATGCT	6	0.15	No Hit
GTAGTATGCAAGATGGTTGGTTCTTTAGTAAATCAATGACTTCTCTGCTG	6	0.15	No Hit
CGGAAATGAACTCTTCAAATCTGATCAGCTGGTTAGCATGATGTAACAAG	6	0.15	No Hit
CCGCAGCATAGCAATCTGGAAAGCTCAGCAAAGGCACAGTGCCCTCGTGG	6	0.15	No Hit
TGGACACACACATCTTCACATTGAGAGCTTCCCAGATTTACAGCCAATTT	6	0.15	No Hit
CTGGGATTGGCAATGTTGAAAGCAAGTGGCGAGGCGGTGTTCAAAAGGTT	6	0.15	No Hit
ATCAAGAGCAAACAGTGTAGGATGGGAGGATGGGAAAGGGGATTCTGGTG	6	0.15	No Hit
TCATCCTGAGTTCGTTCTCTTTTTCGGATACAAATGACCATGTCATCATC	6	0.15	No Hit
GGCTGCAGTGTTTCTCCTACATATTTAGTATAGGCCATGGCAAATTCATG	6	0.15	No Hit
GGCGGTGTTCAAAAGGTTTGGAGACATACTCCTCGGAGACCAAGACTGAG	6	0.15	No Hit
TCTGGGGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAG	6	0.15	No Hit
ATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGTCACAGGTGCAAA	6	0.15	No Hit
GGGTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACCT	6	0.15	No Hit
CAGTGTGTGACCCACAGGTTTAAGGACATGTTTGGCTTCTTGAACAACCA	6	0.15	No Hit
GCCCTCTGAGTGAGGGTCAGTAGACGCTTGGAGCCCATTCCAAGAATCTG	6	0.15	No Hit
TTTTTTTTTTGGGAGACTTTGCTTTATCAACAACAGCATTCGAACTCTTG	6	0.15	No Hit
GCTTCCAACAGTGAACAGTAACAGTAAAGCTAGACACTATTAAAGACGTC	6	0.15	No Hit
TCCCACCTCAACACCCTGGCACTCCCTACACTGGGGAAACAATCCTTCCC	6	0.15	No Hit
GCCAGGAACTTTCCTGTCAACCGGAAAATGGCTCTCTTGCCACTCAATCA	6	0.15	No Hit
CGCTTGTAGGTCATGGCCAGGTCTCTCCAATAGTTTGTTTCTTCCTCCTC	6	0.15	No Hit
CTGGCTGAATGCAGATGGGTAGCTTATTTGGAAGACAGTCCCCTCTCTCT	6	0.15	No Hit
TCCTCCTCTGATCCAAAAGTGTTTCCTGAGTCCTCCATGGTAAAATGGGC	6	0.15	No Hit
TTCTGTGCTTCTGTAAATAACATCATTAAACTCACTGGTCATAGCTAGAC	6	0.15	No Hit
CTCGGAGTTCCTCCTGAGTATTCTCTGCCCTCTGCTTGTAGGTCATGGCC	6	0.15	No Hit
CCAAACATGCTTCCAACAGTGAACAGTAACAGTAAAGCTAGACACTATTA	6	0.15	No Hit
CCCCTGGGTCTTAAACCAAAGAACTAAGAAACAACAAGTAATTACTGCTG	6	0.15	No Hit
CCCCTCTCTATGATATGCAGGTATTACACTTAGTGACTCCTCCCTCTGTC	6	0.15	No Hit
GAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTTCTCTCT	6	0.15	No Hit
TGGTTCTTTAGTAAATCAATGACTTCTCTGCTGAACTGAATCAGCTGAGG	6	0.15	No Hit
GACATAGTTACATAAAAAACAAGGCACACCAATAAGTCACCTAGAAGCCA	6	0.15	No Hit
AGGGGAAAGGTCAAATGAACTTAGCCGGTTTTTCATGTCATTTTTTAACA	6	0.15	No Hit
CTCCACACTACTGTAGCCATAAGTTTTGGGCTGCAGTGTTTCTCCTACAT	6	0.15	No Hit
GCCAATTTGACTCTATTTTTGGGCTGCTTTTTTGCTGGGCTTTCCACTGC	6	0.15	No Hit
CACCCATTCACTGTACACAACTCTTCTTTGTTGCCCACAGAACCTGACCT	6	0.15	No Hit
CCCTCGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTTC	6	0.15	No Hit
CAGGCTGAGTACTTGGAGGTGGTATATTTGGCCAGATTTTAGCAGCATCA	6	0.15	No Hit
TCACCCATTCACTGTACACAACTCTTCTTTGTTGCCCACAGAACCTGACC	6	0.15	No Hit
CTCTCTTTTTCTTCTGTGCAGGAGAGCTGGCAATGCTCTGGGAACTGAGG	6	0.15	No Hit
GTGACTTGTTGGCAGCTCCTGTGGAGAGTGACACCAGTATCTTCTTGCTG	6	0.15	No Hit
TGTAGAATCAGACAGTTTTCCCCCCATCCTAATTTAATTCTGTGTTCAAA	6	0.15	No Hit
AGCCTTAGGACGTCTGCAAAAGAGGCAACTCACACTCCTAACAGCAACTT	6	0.15	No Hit
GGGTTGAGAGGAGTACTGTGAGTGGTTTCATAATGTCTCTTGAGCTCATC	6	0.15	No Hit
TCTGTCAACCGGAAAATGGCTCTCTTGCCACTCAATCATGCCCCAGTGTG	6	0.15	No Hit
CGCTCCACTTCAGTCAAAGTCAGGATTTTCTCTTCTCCACATTCCAACAC	6	0.15	No Hit
CAACAGCGTTGTCGGTGAAAGCTTTGATGAAGCCACGTTTGTTTTTCTGG	6	0.15	No Hit
CCAGGTCTCTCCAATAGTTTGTTTCTTCCTCCTCTGATCCAAAAGTCTTT	5	0.125	No Hit
AATGCCTCCTCGGCAGGCTGCGTGTTGAGCACCAGCACCAGGCAGGCAGG	5	0.125	No Hit
ATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAGAATCAGACAGTTTT	5	0.125	No Hit
GCTCCAGCTGTATATGTAGCAGAGGATGGTCTTATCTCACATCAATGGTG	5	0.125	No Hit
CTGGGCTTTCCACTGCATCCTTGCTAATCAGTAGCCCGGGGGCTTCTGAG	5	0.125	No Hit
TGATCCAAAAGTCTTTCCTGAGTCCTCCATGGTAAAATGGGCAAGAGCTC	5	0.125	No Hit
CACAGACATAGTTACATAAAAAACAAGGCACACCAATAAGTCACCTAGAA	5	0.125	No Hit
CCAGTGTGTGACCCACAGGTTTAAGGACATGTTTGGCTTCTTGAACAACC	5	0.125	No Hit
CCTATGTGTAAGTACTTGGAAGAACATTCAACAACAGGCTTCTGGATGTC	5	0.125	No Hit
CCCGGGTTATCAGTTGGATTTGTTTGCCAGACTCCATATCAGCAACCTTT	5	0.125	No Hit
GGTCTCTCCAATAGTTTGTTTCTTCCTCCTCTGATCCAAAAGTCTTTCCT	5	0.125	No Hit
CGGCGGTGTTCAAAAGGTTTGGAGACATACTCCTCGGAGACCAAGACTGA	5	0.125	No Hit
CCGGTTTTTCATGTCATTTTTTAACACTACAATTCTCTTATGACCATGTC	5	0.125	No Hit
ACCAAGTCTCATCAAGAGCAAACAGTGTAGGATGGGAGGATGGGAAAGGG	5	0.125	No Hit
ACATCATAGCATCATTATCTAAGCCTCTGGATAAAGAAATAGTGATTGGA	5	0.125	No Hit
CTGGGGGGTAGATCGGAAATGAACTCTTCAAATCTGATCAGCTGGTTAGC	5	0.125	No Hit
TTTTTTTCTTTATGTGGCTGGTGCTTTGCAACTTATTATCTGTGTGTCAG	5	0.125	No Hit
CCCAAGTCTCATCAAGAGCAAACAGTGTAGGATGGGAGGATGGGAAAGGG	5	0.125	No Hit
AGAAGCTAAGAAATGTATAATCAGTTCACTTGCCAGTATGAATCCCCACC	5	0.125	No Hit
CCTAGAAGCCAGGAACTTTCCTGTCAACCGGAAAATGGCTCTCTTGCCAC	5	0.125	No Hit
AGCCAGGCCTGCATAGAAAGTACCTTCAGGCATTTCACAAAGAGTGGCAA	5	0.125	No Hit
CTACAAGGTACAGCACAGGGGGGCATCTAGAGCAGCAGCGCTGGAGACTCAAGACATTGAGGAGTAAGACCTCCC	5	0.125	No Hit
GGCTGCTCAATGGGACTCAATCCACTTCCTGCTGAACGATCTCTGCTTAT	5	0.125	No Hit
CCTGTGTGTTATACTTTCTAAAACACCATGTGCTACATATTTGAGCAACA	5	0.125	No Hit
CGCAAAGGCTAGCTTTTCCCAAATGCCAACATCAGCTACCATAATAATAA	5	0.125	No Hit
TGGCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACCTC	5	0.125	No Hit
CTGCCGATTGTAGCCGAAGAAGAGCCGAGGGGGGACAAGTGTGGCCCGAA	5	0.125	No Hit
GCCCATTCCAAGAATCTGCAACACATGAGGCACGGCTTCTAGTAGCTCAA	5	0.125	No Hit
CCCAAATGGAAACTTTTTTAAAAAATGGGATGAGGCCACATTGGTTAACC	5	0.125	No Hit
GGTCACAGGTGCAAAAGAAAAGTTGGCTGCCAATTTGACTCTATTTTTGG	5	0.125	No Hit
CCACAGAACCTGACCTTTGAGATGACTCCAAGTAACTTTAGTGAAACCTA	5	0.125	No Hit
GGCTTCCAAAAGTTCCAGCAACTTGGTAAATCCATAATATGCAAGTTTGC	5	0.125	No Hit
ACAGGCATAGTTACATAAAAAACAAGGCACACCAATAAGTCACCTAGAAG	5	0.125	No Hit
CTCACTCCTAAACCCACAGTTGAGATCCCACTTTTGCTCCACTAACTGTC	5	0.125	No Hit
GGTGCACAGCTCACTACCGCTACTGGCTCCTTTCTCTCAACTAGACTCCT	5	0.125	No Hit
TATCAGGAAGGGGAACTGCTGGGAAAAATTTAGAGCCAACATGAAGGGGA	5	0.125	No Hit
CTTACCCTTATCAAACGGAGACAGACAGAGAAGGGAAACATGAAATAACT	5	0.125	No Hit
CGGCTGTGAGTGATTTTAACTGCATAGACCTGGCCCAGGAGGCTTCAGGG	5	0.125	No Hit
GGTGTCTGAGATCACTGAGTTCTAATGCAAAATTAACATCAGTTGACACA	5	0.125	No Hit
GGGACAGCCTGTAGTCCACATTGCTGACCTGGATGTCAACACCATTTGCA	5	0.125	No Hit
ACCTAGTGTTTTCAAAGTCCATAGTCCCATCTATGCTTGCAAACCTTTAG	5	0.125	No Hit
TCCACTCAGCTGATTTACAATTTATGCATTTAATGCATAGTTTAAAAAAT	5	0.125	No Hit
GGGTGAAGTAGAGGGTTCATTAGGGCAATGAGCACAGCAGCTTACTTTGG	5	0.125	No Hit
TTGCCCCAGTGTGTGACCCACAGGTTTAAGGACATGTTTGGCTTCTTGAA	5	0.125	No Hit
GTCAGAAGTTGATACCAAGGCCTGGGTATTACTGATAGGCCTCACCATAC	5	0.125	No Hit
ACTGCAGTTAAGAGAGCACTTAGACAAGGGTTAGCAGCAAATAAAGAGGA	5	0.125	No Hit
TTTAAATGTATTCAGATTAAACATCTTGGTATATCAAGACATGGTAATAT	5	0.125	No Hit
GTAGCGTTTATGTGAGCAACAGTTACCTGACAATTATTCAGCTCTTGAAT	5	0.125	No Hit
GCCCATTCACTGTACACAACTCTTCTTTGTTGCCCACAGAACCTGACCTT	5	0.125	No Hit
CGCCTGTGATACTTAGCACCTTGCCACCACAGTTATCAGAGAGGCGCCTG	5	0.125	No Hit
CCCATTCACTGTACACAACTCTTCTTTGTTGCCCACAGAACCTGACCTTT	5	0.125	No Hit
TTTTTTTTTGCTTGCTCATAAAGTTTATTGACAACTGTTTGGTCCCAACA	5	0.125	No Hit
TCTCCAATTCAGCTTCATATTCCCGGCTTCCTTCCTGGAATTCTCGGAGT	5	0.125	No Hit
GGAGGCTGCTCAATGGGACTCAATCCACTTCCTGCTGAACGATCTCTGCT	5	0.125	No Hit
CCGCAGTAGGAAGGCTTCTGCGCCAGCAGTCAGGTAGTCTCTGAGCTGGC	5	0.125	No Hit
GGGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAGAATC	5	0.125	No Hit
TACCCTTATCAAACGGAGACAGACAGAGAAGGGAAGCATGAAATAACTTT	5	0.125	No Hit
CTCGTGGGTCTGCAGGAGACTATGAACTTGAGGTGCGAACACTTTCAAAG	5	0.125	No Hit
GCCGGTGCCTGAGATCACTGAGTTCTAATGCAAAATTAACATCAGTTGAC	5	0.125	No Hit
ACCACAATATTTGAAACAGTATCTCCAGTATCTCCTGGTAAACCAAGTTA	5	0.125	No Hit
CCTCCTCTGATCCAAAAGTCTTTCCTGAGTCCTCCATGGTAAAATGGGCA	5	0.125	No Hit
CCTCATCTCAGTTTATAGAAGCACTAACAAATAGTTTGGGATGGAGCTTT	5	0.125	No Hit
CTCAAAACAACTCAGTCTGCTGTCAAGAAAGTAGGATGCTCTCCACAAAC	5	0.125	No Hit
GTACATTATGGCTTCCAGTTTAGTGTTTTTATGGGACTCCTGTGTGCAAG	5	0.125	No Hit
GTTTCTTCTTAGTGGAAATAATATTGAACCCACACCATTTGAAGCACTTC	5	0.125	No Hit
CTCCCTCACACCTGTGTTTTTCAGTGTGGGCACTGAAGCGGTTGGGCCAC	5	0.125	No Hit
GCTGGCCTGGGACTTGAGAAGTTTCAGTAAATCCTGAGTAAAGCGCTTCA	5	0.125	No Hit
GGGGGCAGGTCATAGTGCTGAGAACTGGGAATCACTGGCTTCATTTAAAA	5	0.125	No Hit
AGACTGGTTGCCAACAACGAGGTCTCCTTTTCCATCCCTTTTGTTCCACA	5	0.125	No Hit
GCCCTCTGCTTGTAGGTCATGGCCAGGTCTCTCCAATAGTTTGTTTCTTC	5	0.125	No Hit
CTGGCCTTGCTCTTCTGGACCTGCCATCTTCTCCGATGTTTTGGTCTTTT	5	0.125	No Hit
TCTCCCAAGTCTCATCAAGAGCAAACAGTGTAGGATGGGAGGATGGGAAA	5	0.125	No Hit
CTCCCGTTAGTACTTTGTTCTCCCTTCTGAGAAGGTCTGAAGCACCACAC	5	0.125	No Hit
GGGATTGGCAATGTTGAAAGCAAGTGGCGAGGCGGTGTTCAAAAGGTTTG	5	0.125	No Hit
TTTTTATCAAAATGCTGGCCATGAAATAAGAATCCATTTTCTGGCTTTCT	5	0.125	No Hit
CAGAAAAGAAGTGCAGCCAGGCCTGCATAGAAAGTACCTTCAGGCATTTC	5	0.125	No Hit
CTAGGTCACCAAACTCCGCAGCATAGCAATCTGGAAAGCTCAGCAAAGGC	5	0.125	No Hit
GTCGGTGAAAGCTTTGATGAAGCCGAGTTTGTTTTTCTGGCGGAAGAGGC	5	0.125	No Hit
GTTGCCATTTTGTCCTAATGGCAGTGTCCTTTGTGTCCTTTGTCTTACAG	5	0.125	No Hit
GAGCTTGGTCACAGGTGCAAAAGAAAAGTTGGCTGCCAATTTGACTCTAT	5	0.125	No Hit
ACTCCCCAGATCACACACCCATTCACTGTACACAACTCTTCTTTGTTGCC	5	0.125	No Hit
TGCTCAGCAAAGGCACAGTGCCCTCGTGGGTCTGCAGGAGACTATGAACT	5	0.125	No Hit
CCGGGTAGTGAAGATTGAACTTGGGTTCTAATGCTTGAGAGTCAAGCTCT	5	0.125	No Hit
TACCATTACCTCTCAATACACAATATGTCATTCAAATGCTGACTTAAATG	5	0.125	No Hit
GTGGTAAGAGGGAATGAACTTGTTAAAGGGCATTCGGAAATGGGGCTGGT	5	0.125	No Hit
CCACCACTCAGCTGATTTACAATTTATGCATTTAATGCATAGTTTAAAAA	5	0.125	No Hit
CTCTGACGGAGTTTATCATCAGCAGCATTCTTTGCGGTAGCGTTTATGTG	5	0.125	No Hit
GCTGCAGCCTGTGATACTTAGCACCTTGCCACCACAGTTATCAGAGAGGC	5	0.125	No Hit
GTCCCCTCTCTCTTTTTCTTCTGTGCAGGAGAGCTGGCAATGCTCTGGGA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0125	0.0	0.0	0.0
18-19	0.0	0.05	0.0	0.0	0.0
20-21	0.0	0.05	0.0	0.0	0.0
22-23	0.0	0.05	0.0	0.0	0.0
24-25	0.0	0.05	0.0	0.0	0.0
26-27	0.0	0.05	0.0	0.0	0.0
28-29	0.0	0.05	0.0	0.0	0.0
30-31	0.0	0.05	0.0	0.0	0.0
32-33	0.0	0.05	0.0	0.0	0.0
34-35	0.0	0.05	0.0	0.0	0.0
36-37	0.0	0.225	0.0	0.0	0.0
38-39	0.0	0.225	0.0	0.0	0.0
40-41	0.0	0.25	0.0	0.0	0.0
42-43	0.0	0.25	0.0	0.0	0.0
44-45	0.0	0.25	0.0	0.0	0.0
46-47	0.0	0.25	0.0	0.0	0.0
48-49	0.0	0.25	0.0	0.0	0.0
50-51	0.0	0.25	0.0	0.0	0.0
52-53	0.0	0.25	0.0	0.0	0.0
54-55	0.0	0.25	0.0	0.0	0.0
56-57	0.0	0.25	0.0	0.0	0.0
58-59	0.0	0.25	0.0	0.0	0.0
60-61	0.0	0.25	0.0	0.0	0.0
62-63	0.0	0.25	0.0	0.0	0.0
64-65	0.0	0.3	0.0	0.0	0.0
66-67	0.0	0.35	0.0	0.0	0.0
68-69	0.0	0.525	0.0	0.0	0.0
70-71	0.0	0.7125	0.0	0.0	0.0
72-73	0.0	0.75	0.0	0.0	0.0
74-75	0.0	0.75	0.0	0.0	0.0
76-77	0.0	0.75	0.0	0.0	0.0
78-79	0.0	0.75	0.0	0.0	0.0
80-81	0.0	0.75	0.0	0.0	0.0
82-83	0.0	0.8625	0.0	0.0	0.0
84-85	0.0	0.975	0.0	0.0	0.0
86-87	0.0	0.975	0.0	0.0	0.0
88-89	0.0	0.975	0.0	0.0	0.0
90-91	0.0	0.975	0.0	0.0	0.0
92-93	0.0	0.975	0.0	0.0	0.0
94-95	0.0	0.975	0.0	0.0	0.0
96-97	0.0	0.975	0.0	0.0	0.0
98-99	0.0	0.975	0.0	0.0	0.0
100-101	0.0	0.975	0.0	0.0	0.0
102-103	0.0	0.975	0.0	0.0	0.0
104-105	0.0	0.975	0.0	0.0	0.0
106-107	0.0	1.075	0.0	0.0	0.0
108-109	0.0	1.075	0.0	0.0	0.0
110-111	0.0	1.075	0.0	0.0	0.0
112-113	0.0	1.0875	0.0	0.0	0.0
114-115	0.0	1.1	0.0	0.0	0.0
116-117	0.0	1.1	0.0	0.0	0.0
118-119	0.0	1.1	0.0	0.0	0.0
120-121	0.0	1.1	0.0	0.0	0.0
122-123	0.0	1.1	0.0	0.0	0.0
124-125	0.0	1.1	0.0	0.0	0.0
126-127	0.0	1.1	0.0	0.0	0.0
128-129	0.0	1.1	0.0	0.0	0.0
130-131	0.0	1.1	0.0	0.0	0.0
132-133	0.0	1.1	0.0	0.0	0.0
134-135	0.0	1.1	0.0	0.0	0.0
136-137	0.0	1.1	0.0	0.0	0.0
138-139	0.0	1.15	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 8834862 spots for SRR26281810.sra
Written 8834862 spots for SRR26281810.sra
Read 8834862 spots for SRR26281810.sra
Written 8834862 spots for SRR26281810.sra
Read 8834862 spots for SRR26281810.sra
Written 8834862 spots for SRR26281810.sra
Read 8834862 spots for SRR26281810.sra
Written 8834862 spots for SRR26281810.sra
Read 8834862 spots for SRR26281810.sra
Written 8834862 spots for SRR26281810.sra
Rejected 15964 READS because READLEN < 1
Read 8834867 spots for SRR26281810.sra
Written 8834867 spots for SRR26281810.sra
Read 8834862 spots for SRR26281810.sra
Written 8834862 spots for SRR26281810.sra
Read 8834862 spots for SRR26281810.sra
Written 8834862 spots for SRR26281810.sra
SRR ids: ['SRR26281810.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_7m3f61k8
SRR26281810.sra spots: 70678901
blocks: [[1, 8834862], [8834863, 17669724], [17669725, 26504586], [26504587, 35339448], [35339449, 44174310], [44174311, 53009172], [53009173, 61844034], [61844035, 70678901]]
SRR26281810 file size 21768028
SRR26281810 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR26281810 SRR26281810_1.fastq SRR26281810_2.fastq
Input file:	SRR26281810_1.fastq
Paired file:	SRR26281810_2.fastq
trimmed:	SRR26281810-trimmed-pair1.fastq, SRR26281810-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Mon Nov 10 20:59:45 2025 >> started

Mon Nov 10 21:04:13 2025 >> done (268.372s)
70665429 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
70665429 (100.00%) read pairs available; of these:
  215709 ( 0.31%) trimmed read pairs available after processing
70449720 (99.69%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 35	    1605	  0.00%
 36	    1840	  0.00%
 37	    2392	  0.00%
 38	    3074	  0.00%
 39	    3877	  0.01%
 40	    4841	  0.01%
 41	    5741	  0.01%
 42	    5893	  0.01%
 43	    5678	  0.01%
 44	    5363	  0.01%
 45	    5562	  0.01%
 46	    6221	  0.01%
 47	    7338	  0.01%
 48	    9291	  0.01%
 49	   10739	  0.02%
 50	   13212	  0.02%
 51	   14503	  0.02%
 52	   15124	  0.02%
 53	   15608	  0.02%
 54	   15868	  0.02%
 55	   15970	  0.02%
 56	   16909	  0.02%
 57	   19144	  0.03%
 58	   21691	  0.03%
 59	   25369	  0.04%
 60	   28506	  0.04%
 61	   32394	  0.05%
 62	   35551	  0.05%
 63	   38491	  0.05%
 64	   40474	  0.06%
 65	   41459	  0.06%
 66	   42408	  0.06%
 67	   44049	  0.06%
 68	   47690	  0.07%
 69	   50779	  0.07%
 70	   55220	  0.08%
 71	   63020	  0.09%
 72	   72066	  0.10%
 73	   82881	  0.12%
 74	   90190	  0.13%
 75	   98697	  0.14%
 76	  102549	  0.15%
 77	  104538	  0.15%
 78	  105922	  0.15%
 79	  110233	  0.16%
 80	  118533	  0.17%
 81	  128816	  0.18%
 82	  144806	  0.20%
 83	  162961	  0.23%
 84	  182340	  0.26%
 85	  201325	  0.28%
 86	  211220	  0.30%
 87	  218358	  0.31%
 88	  221766	  0.31%
 89	  223287	  0.32%
 90	  229842	  0.33%
 91	  243209	  0.34%
 92	  263189	  0.37%
 93	  286864	  0.41%
 94	  317278	  0.45%
 95	  344535	  0.49%
 96	  364885	  0.52%
 97	  381593	  0.54%
 98	  384552	  0.54%
 99	  382173	  0.54%
100	  383551	  0.54%
101	  387846	  0.55%
102	  405785	  0.57%
103	  427094	  0.60%
104	  454214	  0.64%
105	  484446	  0.69%
106	  505802	  0.72%
107	  519078	  0.73%
108	  523005	  0.74%
109	  522304	  0.74%
110	  512954	  0.73%
111	  508485	  0.72%
112	  514320	  0.73%
113	  522948	  0.74%
114	  536188	  0.76%
115	  556076	  0.79%
116	  576099	  0.82%
117	  586551	  0.83%
118	  592883	  0.84%
119	  588673	  0.83%
120	  578151	  0.82%
121	  570522	  0.81%
122	  565474	  0.80%
123	  562422	  0.80%
124	  566458	  0.80%
125	  574426	  0.81%
126	  580684	  0.82%
127	  592263	  0.84%
128	  595339	  0.84%
129	  593601	  0.84%
130	  588446	  0.83%
131	  578413	  0.82%
132	  566482	  0.80%
133	  560194	  0.79%
134	  553496	  0.78%
135	  554386	  0.78%
136	  555911	  0.79%
137	  564387	  0.80%
138	  563625	  0.80%
139	  559363	  0.79%
140	  555494	  0.79%
141	  546965	  0.77%
142	  530669	  0.75%
143	  520064	  0.74%
144	  511394	  0.72%
145	  514986	  0.73%
146	  537631	  0.76%
147	  579503	  0.82%
148	 1037532	  1.47%
149	 4314743	  6.11%
150	17712941	 25.07%
151	15219660	 21.54%
70665429 reads passed initial QC


criterion=sequence-density
sequence-density=0.72
sequence-density-rank=1
fanout-score=2.34
fanout-score-rank=41
prefix-density=0.75
prefix-fanout=2.2
sequence=CGAGACAAGACCAT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=45
fanout-score=116.62
fanout-score-rank=1
prefix-density=0.12
prefix-fanout=5.1
sequence=GCCTCTGCTGCTTGAATCCCACCCCTCTATCTGCAGGACCCCGAGCCGTAATGATCCTGCAACAGCCCCTGGAGCGAGGCCCCCCAGGGCGGGACCCACGGGCCACCACTGGGGTGACTCTTGGCCTGGATACCAGGGAACCCTTGCACAAGCACTTCCTGTCTGAGGAGAACATGGCCACCCATTTCTCCCGACTCAGCCTACATAATGACCACCCCTATTGCAGTCCCCCTGTGACTTTTCCCGAAGCCCTGCCACCACTCAGGAGC


criterion=sequence-density
sequence-density=0.81
sequence-density-rank=1
fanout-score=2.13
fanout-score-rank=38
prefix-density=0.83
prefix-fanout=2.1
sequence=ATGGTCTTGTCTCG


criterion=fanout-score
sequence-density=0.02
sequence-density-rank=39
fanout-score=61.30
fanout-score-rank=1
prefix-density=0.40
prefix-fanout=2.5
sequence=TGGGGGCAGGGTGGCTCCATCCAAGGAGTCTCAGCAACAGGAACCCCACAAGGCAACAGTCTACTAATGATTACTATAGAGTTGGGAGACAGGGCTGCTCCACAGACACATGAATAGGTCAAATCTTTTATAAATAAACATCCAGTGAAGAGAAAAATGACAACTCTAATTCATTCTTATACACAAGGCGCACTCTAGGGCGATGGGTGGGGTGACCAGAGGCTGAAATCGTCACTGGTCAGGGGCCTGGGTGGGAGATGAGTGGGCCCAGATGTCCACAGCACGGGGCAGTAGGGGCAAGGCGGGGCAACAGACAATGGGCACTAGGACACTGCACATTTACAAGACCAACTACTGAGGGGGCAGCTGCCCGTGCATGTGTCAGGCTGTTCCTTCTGGAATGAGGAGGGGTGATCTTTACATCCTATCCTGTGACTGGTGGTGGAAAAAGACTACTTTGTGGAGAGGATTAGGGCCACGATGAGACCGTAGAGGCCAAGCACCTCCGCAA
SRR26281810 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Nov 10 21:04:38
                             Started mapping on |	Nov 10 21:04:39
                                    Finished on |	Nov 10 21:15:17
       Mapping speed, Million of reads per hour |	398.74

                          Number of input reads |	70665429
                      Average input read length |	269
                                    UNIQUE READS:
                   Uniquely mapped reads number |	65147024
                        Uniquely mapped reads % |	92.19%
                          Average mapped length |	268.46
                       Number of splices: Total |	70840025
            Number of splices: Annotated (sjdb) |	68825465
                       Number of splices: GT/AG |	69783034
                       Number of splices: GC/AG |	471823
                       Number of splices: AT/AC |	54985
               Number of splices: Non-canonical |	530183
                      Mismatch rate per base, % |	0.43%
                         Deletion rate per base |	0.03%
                        Deletion average length |	1.66
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.70
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	5507041
             % of reads mapped to multiple loci |	7.79%
        Number of reads mapped to too many loci |	5716
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.01%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	11390	11390	11390
N_multimapping	5507041	5507041	5507041
N_noFeature	6882345	7671270	63764897
N_ambiguous	1114725	515164	10209
UnstrandedReadsAssigned:57149954 PositiveStrandReadsAssigned:56960590 NegativeStrandReadsAssigned:1371918
Dataset is classified positive stranded
MeadianReadLen=149 20thPercentileLength=116 echo kmer=111
SRR26281810 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 31,715
[index] number of k-mers: 49,915,185
[index] number of equivalence classes: 70,351
[quant] running in paired-end mode
[quant] will process pair 1: SRR26281810-trimmed-pair1.fastq
                             SRR26281810-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 70,665,429 reads, 60,883,648 reads pseudoaligned
[quant] estimated average fragment length: 175.466
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,246 rounds

  31716 SRR26281810.ke.tsv
  32883 SRR26281810.se.tsv
  64599 total
==> SRR26281810.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	780.534	117932	3537.91
ENSRNOT00000040993.4	1039	864.534	175271	4747.17
ENSRNOT00000050156.3	1545	1370.53	1.00391e+06	17151.9
ENSRNOT00000043693.3	684	509.816	241874	11109.2
ENSRNOT00000046201.3	204	66.4999	62184.6	21896.2
ENSRNOT00000046108.3	681	506.832	306305	14151.4
ENSRNOT00000049683.3	784	609.596	345502	13271.4
ENSRNOT00000041241.3	348	183.94	27799	3538.83
ENSRNOT00000044582.3	297	139.381	24299.6	4082.27

==> SRR26281810.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	45
ENSRNOG00000058808	0
ENSRNOG00000061316	2689
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	37
SRR26281810 completed mapping pipeline successfully
