Starting /dee2/code/volunteer_pipeline.sh SRR26281813
    current disk space = 708178152
    free memory = 199398172 
SRR26281813 SRAfilesize
25ab0e3a812be9b2315eb33c36cc565e  SRR26281813.sra
SRR26281813.sra file validated
SRR26281813 is paired end
SRR26281813 is conventional basespace
SRR26281813 read1 length is 51-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR26281813_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51-151
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.1675	37.0	37.0	37.0	37.0	37.0
2	36.027	37.0	37.0	37.0	37.0	37.0
3	36.2535	37.0	37.0	37.0	37.0	37.0
4	36.239	37.0	37.0	37.0	37.0	37.0
5	36.31	37.0	37.0	37.0	37.0	37.0
6	36.2865	37.0	37.0	37.0	37.0	37.0
7	36.3415	37.0	37.0	37.0	37.0	37.0
8	36.29	37.0	37.0	37.0	37.0	37.0
9	36.3095	37.0	37.0	37.0	37.0	37.0
10-14	36.322900000000004	37.0	37.0	37.0	37.0	37.0
15-19	36.2566	37.0	37.0	37.0	37.0	37.0
20-24	36.2913	37.0	37.0	37.0	37.0	37.0
25-29	36.2054	37.0	37.0	37.0	37.0	37.0
30-34	36.1887	37.0	37.0	37.0	37.0	37.0
35-39	36.1106	37.0	37.0	37.0	37.0	37.0
40-44	36.0723	37.0	37.0	37.0	37.0	37.0
45-49	36.1191	37.0	37.0	37.0	37.0	37.0
50-54	36.049456889222306	37.0	37.0	37.0	37.0	37.0
55-59	36.034058514628654	37.0	37.0	37.0	37.0	37.0
60-64	36.0461769769606	37.0	37.0	37.0	37.0	37.0
65-69	35.99539769884943	37.0	37.0	37.0	37.0	37.0
70-74	35.985421644456125	37.0	37.0	37.0	37.0	37.0
75-79	35.93226312872231	37.0	37.0	37.0	37.0	37.0
80-84	35.92213459096052	37.0	37.0	37.0	37.0	37.0
85-89	35.94434676251983	37.0	37.0	37.0	37.0	37.0
90-94	35.931803027134315	37.0	37.0	37.0	37.0	37.0
95-99	35.94264179453297	37.0	37.0	37.0	37.0	37.0
100-104	35.872424749828646	37.0	37.0	37.0	37.0	37.0
105-109	35.837150916508925	37.0	37.0	37.0	37.0	37.0
110-114	35.79254786930016	37.0	37.0	37.0	37.0	37.0
115-119	35.74670475336882	37.0	37.0	37.0	37.0	37.0
120-124	35.80652742905714	37.0	37.0	37.0	37.0	37.0
125-129	35.58827842273248	37.0	37.0	37.0	37.0	37.0
130-134	35.64839746552334	37.0	37.0	37.0	37.0	37.0
135-139	35.59093751828552	37.0	37.0	37.0	37.0	37.0
140-144	35.40897756012502	37.0	37.0	37.0	34.6	37.0
145-149	35.41679306486934	37.0	37.0	37.0	37.0	37.0
150-151	35.68476862898933	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	2.0
26	3.0
27	11.0
28	31.0
29	40.0
30	46.0
31	72.0
32	96.0
33	121.0
34	190.0
35	365.0
36	2532.0
37	491.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.650000000000006	21.525	15.075	29.75
2	26.588294147073537	22.786393196598297	30.16508254127064	20.460230115057527
3	21.575	23.849999999999998	29.325000000000003	25.25
4	19.675	30.95	27.875	21.5
5	22.2	34.849999999999994	24.55	18.4
6	20.0	33.825	25.974999999999998	20.200000000000003
7	19.925	21.825	37.4	20.849999999999998
8	22.25	22.75	26.700000000000003	28.299999999999997
9	20.275000000000002	21.4	31.125000000000004	27.200000000000003
10-14	22.720000000000002	25.540000000000003	29.060000000000002	22.68
15-19	21.665	27.084999999999997	28.255000000000003	22.994999999999997
20-24	21.39	28.095	26.36	24.154999999999998
25-29	22.415	26.240000000000002	28.050000000000004	23.294999999999998
30-34	22.465	27.985	27.515	22.035
35-39	22.575	26.935	27.62	22.869999999999997
40-44	22.5	25.380000000000003	30.055	22.065
45-49	21.0	28.575	27.22	23.205000000000002
50-54	21.208181227184077	27.429114367155073	29.12936940541081	22.233335000250037
55-59	22.5856464116029	27.156789197299325	27.866966741685424	22.390597649412353
60-64	22.310039517783004	27.082186984142865	28.362763243459554	22.245010254614577
65-69	22.511255627813906	26.633316658329164	28.244122061030513	22.611305652826413
70-74	23.687503127971574	27.511135578799863	28.291877283419247	20.50948400980932
75-79	23.868766930871878	26.683054078458913	26.803451389585632	22.644727601083574
80-84	22.37491190979563	28.32980972515856	28.314708547266687	20.98056981777912
85-89	22.449703542289566	27.26397405361577	28.394060710484975	21.892261693609687
90-94	22.50562257207115	27.187691678593335	27.34103455326109	22.965651196074422
95-99	22.60981912144703	28.361757105943152	27.37467700258398	21.65374677002584
100-104	23.479032847668286	27.317353386599823	27.557574808083974	21.64603895764792
105-109	22.8796424390763	27.279982973289346	27.32254974992019	22.517824837714166
110-114	23.99823866138265	27.031043593130775	26.706296785557022	22.264420959929545
115-119	23.113450426225757	26.208593168945594	28.331140225413353	22.3468161794153
120-124	23.730514879546526	27.031176192725553	26.05101558809636	23.187293339631555
125-129	24.172697470155256	27.01799962887363	25.403599925774728	23.405702975196387
130-134	23.702737940026076	27.69882659713168	25.169491525423727	23.428943937418513
135-139	24.472313596491226	25.699013157894733	27.124451754385966	22.704221491228072
140-144	23.34694462354037	27.40884017479762	26.456049860305182	22.78816534135683
145-149	23.733555118705578	26.62180553455315	26.78058369877514	22.86405564796613
150-151	23.36098474712336	12.229060743912228	36.23227187583623	28.177682633128175
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0
22	1.0
23	1.0
24	2.5
25	10.5
26	22.0
27	16.5
28	12.0
29	18.0
30	19.5
31	30.0
32	47.0
33	48.5
34	48.0
35	88.0
36	109.0
37	86.5
38	91.5
39	114.0
40	126.0
41	167.5
42	222.0
43	241.5
44	272.0
45	296.5
46	299.0
47	242.0
48	183.0
49	162.0
50	141.0
51	136.5
52	122.5
53	103.0
54	82.0
55	64.5
56	58.0
57	60.0
58	77.5
59	76.5
60	52.0
61	30.5
62	12.0
63	7.0
64	6.0
65	4.0
66	0.0
67	5.0
68	8.5
69	4.0
70	0.5
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.05
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	1.0
55-59	0.0
60-64	1.0
65-69	1.0
70-74	4.0
75-79	11.0
80-84	21.0
85-89	38.0
90-94	30.0
95-99	55.0
100-104	27.0
105-109	111.0
110-114	149.0
115-119	119.0
120-124	131.0
125-129	169.0
130-134	165.0
135-139	140.0
140-144	103.0
145-149	530.0
150-152	2194.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.6
#Duplication Level	Percentage of deduplicated	Percentage of total
1	38.45628415300546	14.075
2	20.28688524590164	14.85
3	15.095628415300547	16.575
4	10.1775956284153	14.899999999999999
5	6.693989071038252	12.25
6	2.800546448087432	6.15
7	2.0491803278688523	5.25
8	1.639344262295082	4.8
9	0.819672131147541	2.7
>10	1.9808743169398908	8.450000000000001
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGATGAACTTGCTTTGCTTCTCCCTCGCCAAATAGACATTTCCAAACTTC	16	0.4	No Hit
AGAAATTCCAATACCAGTAAAGAAGGGGAACCTGTCCTGTAACTCCTCAC	16	0.4	No Hit
GTGTGAGTTGCCTCTTTTGCAGACGTCCTAAGGCTAAGCTTGCCTGCTCA	15	0.375	No Hit
GAATTTCCCGAGGCTGCAGGCTGTGGCTGCTCACTCTTCTGGGGGGTTAC	15	0.375	No Hit
AGTTCATTTCCGATCTACCCCCCAGGTTACCACTGAAGATTCCTTGCCAC	15	0.375	No Hit
CCTAAGGCTAAGCTTGCCTGCTCAGACTAAAGGTTTGCAAGCATAGATGG	12	0.3	No Hit
GACACTGCCTTGTGGAATGGCCTTCTCTTCTGGGTGCCTGAGGTATCCAC	12	0.3	No Hit
GCCCAGCAAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGCCAACTTT	12	0.3	No Hit
GTCTGTTCCTTTTCAGAATTTCCCGAGGCTGCAGGCTGTGGCTGCTCACT	12	0.3	No Hit
GGCTAAGCTTGCCTGCTCAGACTAAAGGTTTGCAAGCATAGATGGGACTA	12	0.3	No Hit
GTCTATTCTTCAGGATGCTCCTGCCTGTTGTTTGCCTCTCTTTAAATTTA	11	0.27499999999999997	No Hit
TGCAAGCATAGATGGGACTATGGACTTTGAAAACACTAGGTTTCACTAAA	11	0.27499999999999997	No Hit
CAAAGAATGCTGCTGATGATAAACTCCGTCAGAGCCTGCGAAGGTTTGCA	11	0.27499999999999997	No Hit
GTTCATTTCCGATCTACCCCCCAGGTTACCACTGAAGATTCCTTGCCACA	11	0.27499999999999997	No Hit
CCTCCCTGGGATCTTCGCCGCCCGAGCTGGACATCATGCCACAGGAAGAG	11	0.27499999999999997	No Hit
TGAAAAACACAGGTGTGAGGGAGACACTTCACAGAAGCAGTCAAAAAAAA	11	0.27499999999999997	No Hit
AGAAAGGCCTGCTAGCCCGCGCCTCCCTGGGATCTTCGCCGCCCGAGCTG	11	0.27499999999999997	No Hit
CCGGGACACAGGTCGAGGAACGCTGGCAGCTGGCAACTGCTTTAGCACTG	11	0.27499999999999997	No Hit
CCTGTTGGCACACAGTCTTCCACTAGTCAGATGACTTCTTTCGAGGGAAA	11	0.27499999999999997	No Hit
CCAATGTCAAAATCTTCCAAAGTCCACTCCCTTCTTTTTGAGTCTTCAGT	11	0.27499999999999997	No Hit
CTGACATTTACAATGGATTGTATCTGTTGGGCAAAAAGGGCAAATCCATT	11	0.27499999999999997	No Hit
TGTCCTTAAACCTGTGGGTCACACACTGGGGCATGATTGAGTGGCAAGAG	10	0.25	No Hit
GTGGATTCACAACAAGCCCCCACCTCCCAACACTGACCCTTGGCTTCTTC	10	0.25	No Hit
GGATATTCCAAGCTGATTGAGCTACTAGAAGCCGTGCCTCATGTGTTGCA	10	0.25	No Hit
GGCAAATTCTGGATCTGCTACAAAAAACACAAACTTTAAAGGTTTACAGG	10	0.25	No Hit
CTGGGAAAGGGTTGCAGGCAAATTCTGGATCTGCTACAAAAAACACAAAC	10	0.25	No Hit
CCTTCTCTTCTGGGTGCCTGAGGTATCCACTTATGAGTGGCCTCTCACCG	10	0.25	No Hit
CCAGTAAAGAAGGGGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGC	10	0.25	No Hit
CACTTATGAGTGGCCTCTCACCGCTGTTGGAGAGACCTGACATTTACAAT	10	0.25	No Hit
GAGTTGCCTCTTTTGCAGACGTCCTAAGGCTAAGCTTGCCTGCTCAGACT	9	0.22499999999999998	No Hit
GCTCACTCTTCTGGGGGGTTACTTAGCCGGGACACAGGTCGAGGAACGCT	9	0.22499999999999998	No Hit
CTCCTCACCCAGTAGCCCAGCATCTGCTTCTGCTCCTCCCAAACCCTCCT	9	0.22499999999999998	No Hit
CACGAATCTGTGTGCAAGCAGCACTGCTCGAACAAGGACTTCAGCGAACT	9	0.22499999999999998	No Hit
GATTGAGTGGCAAGAGAGCCATTTTCCGGTTGACAGGAAAGTTCCTGGCT	9	0.22499999999999998	No Hit
GGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCTTCTGCT	9	0.22499999999999998	No Hit
CTTCTCTTCTGGGTGCCTGAGGTATCCACTTATGAGTGGCCTCTCACCGC	9	0.22499999999999998	No Hit
GTGGAAAGCCCAGCAAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGC	9	0.22499999999999998	No Hit
GACTATGGATATTCCAAGCTGATTGAGCTACTAGAAGCCGTGCCTCATGT	9	0.22499999999999998	No Hit
GTTGGAAGCATGTTTGGGTTTTTATTATTATGGTAGCTGATGTTGGCATT	9	0.22499999999999998	No Hit
GTGAGTTGCCTCTTTTGCAGACGTCCTAAGGCTAAGCTTGCCTGCTCAGA	9	0.22499999999999998	No Hit
GGGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCTTCTGC	9	0.22499999999999998	No Hit
GGACTGTCTTCCAAATAAGCTACCCATCTGCATTCAGCCAGCTGATCGCC	8	0.2	No Hit
AGCTGCTTCTAAATTTGGGTCACCAGAAGTTGCAGTCACTGGACAGGTGC	8	0.2	No Hit
AAAAAAAGAGAACCTCAGTTCCCAGAGCATTGCCAGCTCTCCTGCACAGA	8	0.2	No Hit
TGGGACTATGGACTTTGAAAACACTAGGTTTCACTAAAGTTACTTGGAGT	8	0.2	No Hit
GAGGTATCCACTTATGAGTGGCCTCTCACCGCTGTTGGAGAGACCTGACA	8	0.2	No Hit
GCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCCCATTTTACCATGG	8	0.2	No Hit
TTCAGAGCCAGGATGAACTTGCTTTGCTTCTCCCTCGCCAAATAGACATT	8	0.2	No Hit
CTTTACTGTGAAGACTGTCTAAACAAGCCAGCAAGAAATAGTATAATTGA	8	0.2	No Hit
GTTTCAGACTATGGATATTCCAAGCTGATTGAGCTACTAGAAGCCGTGCC	8	0.2	No Hit
GTGCAATTCTTTAAGGCTTCTAAGCAGTGATAAAGTTGCTGTTAGGAGTG	8	0.2	No Hit
CTCCCTTCTTTTTGAGTCTTCAGTTTTCTGGATGGATGTCTGTTCCTTTT	8	0.2	No Hit
CATAGATGGGACTATGGACTTTGAAAACACTAGGTTTCACTAAAGTTACT	8	0.2	No Hit
GGGACTGTCTTCCAAATAAGCTACCCATCTGCATTCAGCCAGCTGATCGC	8	0.2	No Hit
GCATTCGAAGAGTTACAGAAAGGATTGAAGGAGCTGAAGGGGCTTTCAAC	8	0.2	No Hit
TAGAAGCCGTGCCTCATGTGTTGCAGATTCTTGGAATGGGCTCCAAGCGT	8	0.2	No Hit
AAAAAACAGAAATTCCAATACCAGTAAAGAAGGGGAACCTGTCCTGTAAC	8	0.2	No Hit
ATTATTTCCACTAAGAAGAAACCAGAGTTTTATCTGTAATTTGAAGGCCA	8	0.2	No Hit
CATTGGTGTTTCTCAAAGGACTGGGATGTCACGGAATACGGTGTTTGTGA	8	0.2	No Hit
CTTCAGAGCCAGGATGAACTTGCTTTGCTTCTCCCTCGCCAAATAGACAT	8	0.2	No Hit
GGTTTCATGACTTTGACTGAGCTACTGAAGAGTCTGCCTTCTTTAGTAGA	8	0.2	No Hit
ATACAAAATTGGCAGCAAGAAGATACTGGTGTCACTCTCCACAGGAGCTG	8	0.2	No Hit
CTCCTCAGAAGCCCCCGGGCTACTGATTAGCAAGGATGCAGTGGAAAGCC	8	0.2	No Hit
TGCTAAGCCGTGGCTTTGGAAATTCTCTGGTTGCTTTTCTCTTCCTGAGC	8	0.2	No Hit
GGACATCATGCCACAGGAAGAGTGAGCTCTTGCCCATTTTACCATGGAGG	8	0.2	No Hit
GGGATCTTCGCCGCCCGAGCTGGACATCATGCCACAGGAAGAGTGAGCTC	7	0.17500000000000002	No Hit
AAGAACTCCATTAAGGAATCTGTATTATATTTACCAAATTTTTCTAGACT	7	0.17500000000000002	No Hit
GTTTTTTAGAGGACACAGAGAAGCAGAGTTTATCTGTGTGTGTGACATCA	7	0.17500000000000002	No Hit
AGAGCAGCAAACTGTGGACCACACCCCAAGACCATGCTCTAGGCACATGT	7	0.17500000000000002	No Hit
AGTGGATTCACAACAAGCCCCCACCTCCCAACACTGACCCTTGGCTTCTT	7	0.17500000000000002	No Hit
TGATAGCACTTCCTCTTTAAGGCCACATTGCACTTCCTCTGCAATCTTAG	7	0.17500000000000002	No Hit
CAACTATTTGCTTGTCCCAACAAGATGATGACATGGTCATTTGTATCCGA	7	0.17500000000000002	No Hit
ACCTGACATTTACAATGGATTGTATCTGTTGGGCAAAAAGGGCAAATCCA	7	0.17500000000000002	No Hit
CGATGCTAAGCCGTGGCTTTGGAAATTCTCTGGTTGCTTTTCTCTTCCTG	7	0.17500000000000002	No Hit
GTGGATTGAGTCCCATTGAGCAGCCTCCATCATTTGTTTAAGCAAATGTA	7	0.17500000000000002	No Hit
GCCAATGTCAAAATCTTCCAAAGTCCACTCCCTTCTTTTTGAGTCTTCAG	7	0.17500000000000002	No Hit
ATTTAATTAAGAGCTTTCTAGAAAGTTATTTCATGCTTCCCTTCTCTGTC	7	0.17500000000000002	No Hit
GTTTTTATTATTATGGTAGCTGATGTTGGCATTTGGGAAAAGCTAGCCTT	7	0.17500000000000002	No Hit
GTGTCAACTGATGTTAATTTTGCATTAGAACTCAGTGATCTCAGGCACCG	7	0.17500000000000002	No Hit
GTGGACTACAGGCTGTCCCGGAAGGAGCTACAGCAGCTCCTCCAAGAAGC	7	0.17500000000000002	No Hit
GACATGGTCATTTGTATCCGAAAAAGAGAACGAACTCAGGATGAAATAGA	7	0.17500000000000002	No Hit
ACAACTATTTGCTTGTCCCAACAAGATGATGACATGGTCATTTGTATCCG	7	0.17500000000000002	No Hit
AGGAGCTGGTGGCTGAGGGTGGAAGAATGAAAGAAAAGAAAGATGAAAGA	7	0.17500000000000002	No Hit
CGCTGTGGAACAAAAGGGATGGAAAAGGAGACCTCGTTGTTGGCAACCAG	7	0.17500000000000002	No Hit
GCCAACTTTTCTTTTGCACCTGTGACCAAGCTCTAACTTCCAGTTTGAAC	7	0.17500000000000002	No Hit
AAATGGATTCTTATTTCATGGCCAGCATTTTGATAAAAATGTCACAAAAT	7	0.17500000000000002	No Hit
TTCCAAACTTCCCTTTTCCTAGTGGGCGGCCAATGTCAAAATCTTCCAAA	7	0.17500000000000002	No Hit
CCTGGGAAAGGGTTGCAGGCAAATTCTGGATCTGCTACAAAAAACACAAA	7	0.17500000000000002	No Hit
ATCTATGAAAAGAAGTATGGACACAAGTTAAATGTGTCTGATTTATATAA	7	0.17500000000000002	No Hit
ATGGATGTCTGTTCCTTTTCAGAATTTCCCGAGGCTGCAGGCTGTGGCTG	7	0.17500000000000002	No Hit
GCTGTTAGGAGTGTGAGTTGCCTCTTTTGCAGACGTCCTAAGGCTAAGCT	7	0.17500000000000002	No Hit
CATGTTTGGGTTTTTATTATTATGGTAGCTGATGTTGGCATTTGGGAAAA	7	0.17500000000000002	No Hit
GCAAGAAAGGCCTGCTAGCCCGCGCCTCCCTGGGATCTTCGCCGCCCGAG	7	0.17500000000000002	No Hit
CCAGCTACTGTGGTTCTTGTGTCAACTGATGTTAATTTTGCATTAGAACT	7	0.17500000000000002	No Hit
CTTCAGCGAACTTGTATTTGATCCAGACTCTTACAAGGTTCCTTTTGTGG	7	0.17500000000000002	No Hit
AGTTATTTGAAAGAACTCCATTAAGGAATCTGTATTATATTTACCAAATT	6	0.15	No Hit
CCCATCTGCATTCAGCCAGCTGATCGCCTCAAGGCAAGTCAGTCCTCTGT	6	0.15	No Hit
GTGAACAGCCTCCACAGATACAAAATTGGCAGCAAGAAGATACTGGTGTC	6	0.15	No Hit
GGAAAAGGAGACCTCGTTGTTGGCAACCAGTCTAGGCAAAGCTGCTTCTA	6	0.15	No Hit
ATTTCTCACAGGCTTATCATTGGTGTTTCTCAAAGGACTGGGATGTCACG	6	0.15	No Hit
TGTGTGTCCATGTTAGTTCATCTTGACGTCTTTAATAGTGTCTAGCTTTA	6	0.15	No Hit
ACTGAAAAACACAGGTGTGAGGGAGACACTTCACAGAAGCAGTCAAAAAA	6	0.15	No Hit
TAGCCGGGACACAGGTCGAGGAACGCTGGCAGCTGGCAACTGCTTTAGCA	6	0.15	No Hit
CTTCTTTTCTGAGGCCTGATAGCACTTCCTCTTTAAGGCCACATTGCACT	6	0.15	No Hit
TTTTAAACCAAGAAGAAAAAAACAGAAATTCCAATACCAGTAAAGAAGGG	6	0.15	No Hit
GCATGTTTGGGTTTTTATTATTATGGTAGCTGATGTTGGCATTTGGGAAA	6	0.15	No Hit
GTTCCTTTTCAGAATTTCCCGAGGCTGCAGGCTGTGGCTGCTCACTCTTC	6	0.15	No Hit
CAGGTCATTGTGAGCGATTTCTCACAGGCTTATCATTGGTGTTTCTCAAA	6	0.15	No Hit
GTTCATCTTGACGTCTTTAATAGTGTCTAGCTTTACTGTTACTGTTCACT	6	0.15	No Hit
CAGTAGCCCAGCATCTGCTTCTGCTCCTCCCAAACCCTCCTCAGAAGCCC	6	0.15	No Hit
GTACTTTAAACAGCATCTTCAGAGCCAGGATGAACTTGCTTTGCTTCTCC	6	0.15	No Hit
AAGATACTGGTGTCACTCTCCACAGGAGCTGCCAACAAGTCACTCTCTTT	6	0.15	No Hit
CTTTAAAGGTTTACAGGAGCTGTGCCGAATGGAACCAAAGTCTGGCAATA	6	0.15	No Hit
CTCAAGTTCATAGTCTCCTGCAGACCCACGAGGGCACTGTGCCTTTGCTG	6	0.15	No Hit
GTTAACCAATGTGGCCTCATCCCATTTTTTAAAAAAGTTTCCATTTGGGG	6	0.15	No Hit
ATGATGACATGGTCATTTGTATCCGAAAAAGAGAACGAACTCAGGATGAA	6	0.15	No Hit
TAAAGGTTTGCAAGCATAGATGGGACTATGGACTTTGAAAACACTAGGTT	6	0.15	No Hit
CTTCGCCGCCCGAGCTGGACATCATGCCACAGGAAGAGTGAGCTCTTGCC	6	0.15	No Hit
GCTGAATAATTGTCAGGTAACTGTTGCTCACATAAACGCTACCGCAAAGA	6	0.15	No Hit
GATAAAAGGACATGGTCATAAGAGAATTGTAGTGTTAAAAAATGACATGA	6	0.15	No Hit
CCCAGACACAACTATTTGCTTGTCCCAACAAGATGATGACATGGTCATTT	6	0.15	No Hit
CTTTTCTTTTGCACCTGTGACCAAGCTCTAACTTCCAGTTTGAACACAGA	6	0.15	No Hit
CTCAATTGGAAGCTCTCAGAGCGTGACATGCCCAAGAGGCCAGTCCAATC	6	0.15	No Hit
CCCGGAAGGAGCTACAGCAGCTCCTCCAAGAAGCCTTCTCCAAGCATGGC	6	0.15	No Hit
CCTCAGAAGCCCCCGGGCTACTGATTAGCAAGGATGCAGTGGAAAGCCCA	6	0.15	No Hit
CCAACTCTCTCCACCTAAATGTGGCACCTCCGGTTTGTTTAAAAGGCTCA	6	0.15	No Hit
GGTTTACAGGAGCTGTGCCGAATGGAACCAAAGTCTGGCAATAGAATCCG	6	0.15	No Hit
AAATCTGGGAAGCTCTCAATGTGAAGATGTGTGTGTCCATGTTAGTTCAT	6	0.15	No Hit
TGTCTATTCTTCAGGATGCTCCTGCCTGTTGTTTGCCTCTCTTTAAATTT	6	0.15	No Hit
GATTGAGTCCCATTGAGCAGCCTCCATCATTTGTTTAAGCAAATGTATTT	6	0.15	No Hit
TGCAGTGGAAAGCCCAGCAAAAAAGCAGCCCAAAAATAGAGTCAAATTGG	6	0.15	No Hit
CCTCTTACCACCACCACTTTGGCCGGCAGTGCAAACTTGCATATTATGGA	6	0.15	No Hit
CGTCAAAGTAGTGAAGTGGATTCACAACAAGCCCCCACCTCCCAACACTG	6	0.15	No Hit
GTCCACTCCCTTCTTTTTGAGTCTTCAGTTTTCTGGATGGATGTCTGTTC	6	0.15	No Hit
GCTCAGACTAAAGGTTTGCAAGCATAGATGGGACTATGGACTTTGAAAAC	6	0.15	No Hit
TTCGTCTCCAAGACTATGATGTCAGTTCAGTCTCAGCTTTGACACAGAAG	6	0.15	No Hit
GATGAACTTTTTCTTACTTTATTTCTTCCAGTTATTTGAAAGAACTCCAT	5	0.125	No Hit
AGGAAAGTTCCTGGCTTCTAGGTGACTTATTGGTGTGCCTTGTTTTTTAT	5	0.125	No Hit
CGTTCAGCAGGAAGTGGATTGAGTCCCATTGAGCAGCCTCCATCATTTGT	5	0.125	No Hit
TTCCAATACCAGTAAAGAAGGGGAACCTGTCCTGTAACTCCTCACCCAGT	5	0.125	No Hit
GTCTCAGCTTTGACACAGAAGCTCTGCCATGTTGTAAAGGTTGCTGATAT	5	0.125	No Hit
GTAAAGAAGGGGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATC	5	0.125	No Hit
ATTTTACTCACTTTACAAGTAAATTGATTGGATTAAGAACTGAAGCAATG	5	0.125	No Hit
CCAAGCTCTAACTTCCAGTTTGAACACAGAATTAAATTAGGATGGGGGGA	5	0.125	No Hit
AGAAAGTTATTTCATGCTTCCCTTCTCTGTCTGTCTCCGTTTGATAAGGG	5	0.125	No Hit
GTGGTGAAGTGCTTCAAATGGTGTGGGTTCAATATTATTTCCACTAAGAA	5	0.125	No Hit
AGCTTGCCTGCTCAGACTAAAGGTTTGCAAGCATAGATGGGACTATGGAC	5	0.125	No Hit
AATTTCCAGGGTGAAGGCAGGAGGATCAGAAAAGCTCAAGGTCACATTTC	5	0.125	No Hit
CTGCTGTCACCTGAGTTTTTTATCTTAGCCATTCTGCCTGGTGTGAGGTG	5	0.125	No Hit
GGTGTTGACATCCAGGTCAGCAATGTGGACTACAGGCTGTCCCGGAAGGA	5	0.125	No Hit
CTCCGGTTTGTTTAAAAGGCTCACTTTACTGTGAAGACTGTCTAAACAAG	5	0.125	No Hit
GGAGCTGGTGGCTGAGGGTGGAAGAATGAAAGAAAAGAAAGATGAAAGAA	5	0.125	No Hit
CCTGCTCAGACTAAAGGTTTGCAAGCATAGATGGGACTATGGACTTTGAA	5	0.125	No Hit
CTGTGCTCTTAACCGCTGAGTCCTCTCTCCAGCCCCTGCAGAGTACTAAG	5	0.125	No Hit
AACACTGCAGCCCAAAACTTATGGCTACAGTAGTGTGGAGGAGCTACTGG	5	0.125	No Hit
CACAACTATTTGCTTGTCCCAACAAGATGATGACATGGTCATTTGTATCC	5	0.125	No Hit
GAAGAAACCAGAGTTTTATCTGTAATTTGAAGGCCAAGGTTGATCCTTGT	5	0.125	No Hit
GTCAAAATCTTCCAAAGTCCACTCCCTTCTTTTTGAGTCTTCAGTTTTCT	5	0.125	No Hit
TGACTGGCCTGCTGTTTACATTTCTCCTTTGGTAGCATACAGAATACCTT	5	0.125	No Hit
GCACTTCCTCTGCAATCTTAGTGCACATGTGCAATTCTTAAGGCTTCTAA	5	0.125	No Hit
CTCTGAAGCCTTGTTACATCATGCTAACCAGCTGATCAGATTTGAAGAGT	5	0.125	No Hit
GGGCGGCCAATGTCAAAATCTTCCAAAGTCCACTCCCTTCTTTTTGAGTC	5	0.125	No Hit
GGGGAACCTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCTTCTG	5	0.125	No Hit
AAAAACAGAAATTCCAATACCAGTAAAGAAGGGGAACCTGTCCTGTAACT	5	0.125	No Hit
TTCATGCTTCCCTTCTCTGTCTGTCTCCGTTTGATAAGGGTAAGGTTTAA	5	0.125	No Hit
CCACTCCCTTCTTTTTGAGTCTTCAGTTTTCTGGATGGATGTCTGTTCCT	5	0.125	No Hit
GTTTATATTATTTTTTCTAGAAAGCCAGAAAATGGATTCTTATTTCATGG	5	0.125	No Hit
GTGGAGGAGCTACTGGGAGCCATCCCACAGGTGGTCTGGATAAAAGGACA	5	0.125	No Hit
AAGCCATACCTGAGATTTTACAAGTGTTGGAATGTGGAGAAGAGAAAATC	5	0.125	No Hit
GCTAAGCTTGCCTGCTCAGACTAAAGGTTTGCAAGCATAGATGGGACTAT	5	0.125	No Hit
CAAGAAGCCAAACATGTCCTTAAACCTGTGGGTCACACACTGGGGCATGA	5	0.125	No Hit
TTATATTATTTTTTCTAGAAAGCCAGAAAATGGATTCTTATTTCATGGCC	5	0.125	No Hit
GGAAAGCCCAGCAAAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGCC	5	0.125	No Hit
ACACACATATACATTTTTAATGAACAAAGAGCCAAAGTAATTTAATAGAC	5	0.125	No Hit
GGGGACTGTCTTCCAAATAAGCTACCCATCTGCATTCAGCCAGCTGATCG	5	0.125	No Hit
TTTTTTTTGAGTCTTCAGTTTTCTGGATGGATGTCTGTTCCTTTTCAGAA	5	0.125	No Hit
CAAGCAGGTCATTGTGAGCGATTTCTCACAGGCTTATCATTGGTGTTTCT	5	0.125	No Hit
CATTTAAGTCAGCATTGAATGACATATTGTGTATTGAGAGGTAATGGTTA	5	0.125	No Hit
AGCTAGCCTTTGCGGACACTGCCTTGTGGAATGGCCTTCTCTTCTGGGTG	5	0.125	No Hit
TCATCCCATTTTTTAAAAAAGTTTCCATTTGGGGATTCCATTTAATTAAG	5	0.125	No Hit
AATACGCACACTGCTCCAGCTACTGTGGTTCTTGTGTCAACTGATGTTAA	5	0.125	No Hit
TGAGTCTTCAGTTTTCTGGATGGATGTCTGTTCCTTTTCAGAATTTCCCG	5	0.125	No Hit
CTGGGATCTTCGCCGCCCGAGCTGGACATCATGCCACAGGAAGAGTGAGC	5	0.125	No Hit
GGCTGCTCACTCTTCTGGGGGGTTACTTAGCCGGGACACAGGTCGAGGAA	5	0.125	No Hit
CTCCCCAATCTGTTCAATCTGTGGGAAGGTCTCTTCCCACAGAATCTTTT	5	0.125	No Hit
CTGAAAGCCATCGTGCAGATGAGAAACCTGCATGACGCAATCTGTGCTGT	5	0.125	No Hit
GGTTCCGAAAGGGCTGGTCGGCAAGAAAGGCCTGCTAGCCCGCGCCTCCC	5	0.125	No Hit
GCACAGAATCATATGCAGACAAAATGCCCATACACATAAAATAACTTTTT	5	0.125	No Hit
AAGCTAGCCTTTGCGGACACTGCCTTGTGGAATGGCCTTCTCTTCTGGGT	5	0.125	No Hit
GGGAGACCAACTCTCTCTGGGCAGGTTTTGGGTTCGAAAGGCTGTGCCAA	5	0.125	No Hit
CCCACAGGTGGTCTGGATAAAAGGACATGGTCATAAGAGAATTGTAGTGT	5	0.125	No Hit
GCTACTGGGAGCCATCCCACAGGTGGTCTGGATAAAAGGACATGGTCATA	5	0.125	No Hit
CTCCACACCTGCTTCTCCAGCTGTATTTTAAACAGCATCTTCAGAGCCAG	5	0.125	No Hit
GAGATTTTTTTTACCAGTTGAATTTTTTTAATCAATATCTTTTAAATGAA	5	0.125	No Hit
AATGTCAAAATCTTCCAAAGTCCACTCCCTTCTTTTTGAGTCTTCAGTTT	5	0.125	No Hit
GTCTCTTCCCACAGAATCTTTTATGGTTTCCTATCCGACTGCCAGAGAAC	5	0.125	No Hit
TGTCCCGGAAGGAGCTACAGCAGCTCCTCCAAGAAGCCTTCTCCAAGCAT	5	0.125	No Hit
GTCATTTGTATCCGAAAAAGAGAACGAACTCAGGATGAAATAGAAAGGAC	5	0.125	No Hit
GGGAAAAGCTAGCCTTTGCGGACACTGCCTTGTGGAATGGCCTTCTCTTC	5	0.125	No Hit
CAATTTCATTGCTAGGGATCTACAATGTTCCCTATAAGAGTTGTGTAGAA	5	0.125	No Hit
CTATGATGTATGTACATGGAGATAATGAGACACTTTTTCTGGGTTGAAGG	5	0.125	No Hit
TCCAAACTTCCCTTTTCCTAGTGGGCGGCCAATGTCAAAATCTTCCAAAG	5	0.125	No Hit
GACATTTCCAAACTTCCCTTTTCCTAGTGGGCGGCCAATGTCAAAATCTT	5	0.125	No Hit
TGAAGATTCCTTGCCACACTCTACTCTATGTTTATAACCTGCCGGCAAAT	5	0.125	No Hit
GCAGGCTGTGGCTGCTCACTCTTCTGGGGGGTTACTTAGCCGGGACACAG	5	0.125	No Hit
TGGTCATAAGAGAATTGTAGTGTTAAAAAATGACATGAAAAACCGGCTAA	5	0.125	No Hit
TGTGGAGGAGCTACTGGGAGCCATCCCACAGGTGGTCTGGATAAAAGGAC	5	0.125	No Hit
TCTTTGTGAAATGCCTGAAGGTACTTTCTATGCAGGCCTGGCTGCACTTC	5	0.125	No Hit
GATTTTTCTTCATGAATTTGCCATGGCCTATACTAAATATGTAGGAGAAA	5	0.125	No Hit
CCTGAAGGTACTTTCTATGCAGGCCTGGCTGCACTTCTTTTCTGAGGCCT	5	0.125	No Hit
CCTCGTTGTTGGCAACCAGTCTAGGCAAAGCTGCTTCTAAATTTGGGTCA	5	0.125	No Hit
GGAAGTCATTCAAGAGCTGAATAATTGTCAGGTAACTGTTGCTCACATAA	5	0.125	No Hit
CAATGTCAAAATCTTCCAAAGTCCACTCCCTTCTTTTTGAGTCTTCAGTT	5	0.125	No Hit
CAATTCTGATGCATTGTCTGCAGAGAGGAATCCCTTGTCTCACCCGGCTT	5	0.125	No Hit
CTGTCCTGTAACTCCTCACCCAGTAGCCCAGCATCTGCTTCTGCTCCTCC	5	0.125	No Hit
TGGTGCTCCACACCTGCTTCTCCAGCTGTACTTTAAACAGCATCTTCAGA	5	0.125	No Hit
CAAGAAGATACTGGTGTCACTCTCCACAGGAGCTGCCAACAAGTCACTCT	5	0.125	No Hit
TGCTGTCATTCTTTTTCTTGGACATGTAACCCTAGGCAAGCTGTGTGCCT	5	0.125	No Hit
CAAAACTTATGGCTACAGTAGTGTGGAGGAGCTACTGGGAGCCATCCCAC	5	0.125	No Hit
CCAGATCCTGTCATCCTGCAGCCCACTGATCTCATTCAGTTTGAGGAACA	5	0.125	No Hit
TGCTCAGACTAAAGGTTTGCAAGCATAGATGGGACTATGGACTTTGAAAA	5	0.125	No Hit
GTAGTGTGGAGGAGCTACTGGGAGCCATCCCACAGGTGGTCTGGATAAAA	5	0.125	No Hit
GCCTGCTAGCCCGCGCCTCCCTGGGATCTTCGCCGCCCGAGCTGGACATC	5	0.125	No Hit
CTGAGGTATCCACTTATGAGTGGCCTCTCACCGCTGTTGGAGAGACCTGA	5	0.125	No Hit
GGGCACTGTGCCTTTGCTGAGCTTTCCAGATTGCTATGCTGCGGAGTTTG	5	0.125	No Hit
GGCTCCTGGTTTGCTACTAGCACCTCTGTATTGGACATGCTCTGGATGTG	5	0.125	No Hit
GCCTGAAGGTACTTTCTATGCAGGCCTGGCTGCACTTCTTTTCTGAGGCC	5	0.125	No Hit
GTCCTTAAACCTGTGGGTCACACACTGGGGCATGATTGAGTGGCAAGAGA	5	0.125	No Hit
GAGCTACTGAAGAGTCTGCCTTCTTTAGTAGAGGTTTTTATGAATGATAA	5	0.125	No Hit
CAAAATCTTCCAAAGTCCACTCCCTTCTTTTTGAGTCTTCAGTTTTCTGG	5	0.125	No Hit
TACCTCTTGTCTCCCAAGTCTCATCAAGAGCAAACAGTGTAGGATGGGAG	5	0.125	No Hit
CATTGATCACATGTTAACCTCTCACTTTATTTACATTTTAAATACTTTTT	5	0.125	No Hit
GTCCATGTTAGTTCATCTTGACGTCTTTAATAGTGTCTAGCTTTACTGTT	5	0.125	No Hit
CCACTTATGAGTGGCCTCTCACCGCTGTTGGAGAGACCTGACATTTACAA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0125	0.0	0.0	0.0
70-71	0.0	0.025	0.0	0.0	0.0
72-73	0.0	0.025	0.0	0.0	0.0
74-75	0.0	0.025	0.0	0.0	0.0
76-77	0.0	0.025	0.0	0.0	0.0
78-79	0.0	0.025	0.0	0.0	0.0
80-81	0.0	0.025	0.0	0.0	0.0
82-83	0.0	0.025	0.0	0.0	0.0
84-85	0.0	0.025	0.0	0.0	0.0
86-87	0.0	0.025	0.0	0.0	0.0
88-89	0.0	0.025	0.0	0.0	0.0
90-91	0.0	0.025	0.0	0.0	0.0
92-93	0.0	0.025	0.0	0.0	0.0
94-95	0.0	0.025	0.0	0.0	0.0
96-97	0.0	0.025	0.0	0.0	0.0
98-99	0.0	0.025	0.0	0.0	0.0
100-101	0.0	0.025	0.0	0.0	0.0
102-103	0.0	0.025	0.0	0.0	0.0
104-105	0.0	0.025	0.0	0.0	0.0
106-107	0.0	0.025	0.0	0.0	0.0
108-109	0.0	0.025	0.0	0.0	0.0
110-111	0.0	0.025	0.0	0.0	0.0
112-113	0.0	0.025	0.0	0.0	0.0
114-115	0.0	0.025	0.0	0.0	0.0
116-117	0.0	0.025	0.0	0.0	0.0
118-119	0.0	0.025	0.0	0.0	0.0
120-121	0.0	0.025	0.0	0.0	0.0
122-123	0.0	0.025	0.0	0.0	0.0
124-125	0.0	0.025	0.0	0.0	0.0
126-127	0.0	0.025	0.0	0.0	0.0
128-129	0.0	0.025	0.0	0.0	0.0
130-131	0.0	0.025	0.0	0.0	0.0
132-133	0.0	0.025	0.0	0.0	0.0
134-135	0.0	0.025	0.0	0.0	0.0
136-137	0.0	0.025	0.0	0.0	0.0
138-139	0.0	0.025	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTTTT	10	0.008573525	134.3875	9
>>END_MODULE
SRR26281813 read2 length is 40-151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR26281813_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	40-151
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.1185	37.0	37.0	37.0	37.0	37.0
2	36.237	37.0	37.0	37.0	37.0	37.0
3	36.261	37.0	37.0	37.0	37.0	37.0
4	36.304	37.0	37.0	37.0	37.0	37.0
5	36.293	37.0	37.0	37.0	37.0	37.0
6	36.181	37.0	37.0	37.0	37.0	37.0
7	36.0515	37.0	37.0	37.0	37.0	37.0
8	36.4075	37.0	37.0	37.0	37.0	37.0
9	36.194	37.0	37.0	37.0	37.0	37.0
10-14	36.2947	37.0	37.0	37.0	37.0	37.0
15-19	36.261100000000006	37.0	37.0	37.0	37.0	37.0
20-24	36.206900000000005	37.0	37.0	37.0	37.0	37.0
25-29	36.2351	37.0	37.0	37.0	37.0	37.0
30-34	36.100199999999994	37.0	37.0	37.0	37.0	37.0
35-39	36.0926	37.0	37.0	37.0	37.0	37.0
40-44	36.113886628873324	37.0	37.0	37.0	37.0	37.0
45-49	36.06245016428655	37.0	37.0	37.0	37.0	37.0
50-54	36.0640600570548	37.0	37.0	37.0	37.0	37.0
55-59	36.02312312312312	37.0	37.0	37.0	37.0	37.0
60-64	35.98898625784733	37.0	37.0	37.0	37.0	37.0
65-69	35.97702127659574	37.0	37.0	37.0	37.0	37.0
70-74	35.955723314430564	37.0	37.0	37.0	37.0	37.0
75-79	36.005006701649684	37.0	37.0	37.0	37.0	37.0
80-84	35.87943173568439	37.0	37.0	37.0	37.0	37.0
85-89	35.88503351417105	37.0	37.0	37.0	37.0	37.0
90-94	35.82561409376216	37.0	37.0	37.0	37.0	37.0
95-99	35.813781617880835	37.0	37.0	37.0	37.0	37.0
100-104	35.80006750638813	37.0	37.0	37.0	37.0	37.0
105-109	35.776308346927124	37.0	37.0	37.0	37.0	37.0
110-114	35.71194586678753	37.0	37.0	37.0	37.0	37.0
115-119	35.6942562912022	37.0	37.0	37.0	37.0	37.0
120-124	35.7109984121462	37.0	37.0	37.0	37.0	37.0
125-129	35.62195199251374	37.0	37.0	37.0	37.0	37.0
130-134	35.40027092598533	37.0	37.0	37.0	37.0	37.0
135-139	35.2601952029945	37.0	37.0	37.0	32.2	37.0
140-144	35.4003314941359	37.0	37.0	37.0	37.0	37.0
145-149	35.1336426354796	37.0	37.0	37.0	25.0	37.0
150-151	35.622351950336544	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	0.0
23	1.0
24	5.0
25	7.0
26	9.0
27	11.0
28	22.0
29	33.0
30	55.0
31	61.0
32	84.0
33	125.0
34	160.0
35	426.0
36	2609.0
37	391.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.675	9.8	16.325	43.2
2	23.05	6.0249999999999995	32.775	38.15
3	20.575	10.75	23.375	45.300000000000004
4	24.25	13.4	21.675	40.675
5	24.0	23.400000000000002	24.325	28.275
6	28.125	26.924999999999997	20.7	24.25
7	18.925	30.825000000000003	37.775	12.475
8	22.05	25.275	29.775000000000002	22.900000000000002
9	22.475	24.4	31.674999999999997	21.45
10-14	21.43	29.665000000000003	27.48	21.425
15-19	21.205	28.585	26.729999999999997	23.48
20-24	21.634999999999998	26.740000000000002	28.599999999999998	23.025000000000002
25-29	24.21	27.355	26.005	22.43
30-34	21.884999999999998	27.139999999999997	26.575	24.4
35-39	22.705000000000002	26.005	28.455000000000002	22.835
40-44	23.013054569099182	27.02946031110889	28.559995998599508	21.397489121192418
45-49	23.01110777544281	26.038226758731113	27.309116381467025	23.641549084359053
50-54	23.806425783204883	28.23040736662997	24.66720048043239	23.29596636973276
55-59	21.12112112112112	26.826826826826828	28.43843843843844	23.613613613613612
60-64	22.3968762515018	29.390268321986383	25.555666800160193	22.65718862635162
65-69	23.589486858573217	28.08010012515644	27.274092615769714	21.056320400500624
70-74	22.778723830511872	27.06601222077532	26.324752078533507	23.830511870179304
75-79	21.662734072995633	28.254430443295348	27.54154325016316	22.54129223354586
80-84	21.656339730995917	27.016271220593417	27.97843937333132	23.348949675079343
85-89	21.294511514659632	29.791011463934257	27.69605356599371	21.218423455412395
90-94	21.916896939924264	29.95599222188108	26.466073073380414	21.661037764814246
95-99	22.07046406953283	27.280252470381296	27.497542552641107	23.151740907444772
100-104	21.468187526161575	27.344077019673502	30.237547090832983	20.950188363331936
105-109	24.142529471382087	28.436549847975677	27.727103003147167	19.693817677495066
110-114	23.443708609271525	24.950331125827816	27.417218543046356	24.188741721854303
115-119	22.269966769794888	24.676291967457317	28.217027615446316	24.83671364730148
120-124	21.402388553860707	29.18292538725316	26.823932836703325	22.59075322218281
125-129	23.617561458913862	24.373026193572358	29.04823828100811	22.961174066505667
130-134	23.670993412040964	27.819450785989176	26.86060922314265	21.648946578827214
135-139	21.63458244844831	26.841131739398506	26.800027402891004	24.724258409262177
140-144	20.24926581190459	27.684263304920854	27.562495523243317	24.50397535993124
145-149	22.968891573542734	29.636061612805797	24.773482331621864	22.621564482029598
150-151	22.059973924380706	13.741851368970012	38.148631029986966	26.049543676662324
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.5
23	1.0
24	0.5
25	0.5
26	4.5
27	10.0
28	10.5
29	13.5
30	21.5
31	24.0
32	50.5
33	70.0
34	70.5
35	99.5
36	86.5
37	71.5
38	107.5
39	100.0
40	96.5
41	143.0
42	175.0
43	174.5
44	226.5
45	311.5
46	265.5
47	180.0
48	167.0
49	168.5
50	186.0
51	187.5
52	166.0
53	135.0
54	96.0
55	76.0
56	81.0
57	100.5
58	119.5
59	96.5
60	52.5
61	37.5
62	19.5
63	6.0
64	3.5
65	1.0
66	1.5
67	2.0
68	3.5
69	5.0
70	3.5
71	1.5
72	0.5
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
40-44	2.0
45-49	1.0
50-54	1.0
55-59	0.0
60-64	1.0
65-69	1.0
70-74	4.0
75-79	11.0
80-84	21.0
85-89	39.0
90-94	31.0
95-99	55.0
100-104	30.0
105-109	114.0
110-114	143.0
115-119	119.0
120-124	130.0
125-129	167.0
130-134	162.0
135-139	140.0
140-144	105.0
145-149	427.0
150-152	2296.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.0
#Duplication Level	Percentage of deduplicated	Percentage of total
1	46.381578947368425	17.625
2	18.75	14.249999999999998
3	13.88157894736842	15.825
4	8.289473684210526	12.6
5	4.2105263157894735	8.0
6	3.289473684210526	7.5
7	1.1842105263157896	3.15
8	1.118421052631579	3.4000000000000004
9	0.6578947368421052	2.25
>10	2.236842105263158	15.4
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTCCCCAGATCACACACCCATTCACTGTACACAACTCTTCTTTGTTGCCC	47	1.175	No Hit
CCCCAGATCACACACCCATTCACTGTACACAACTCTTCTTTGTTGCCCAC	47	1.175	No Hit
CTCGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTTCTC	46	1.15	No Hit
CCCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGTC	40	1.0	No Hit
CCTCGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTTCT	37	0.9249999999999999	No Hit
GTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACCTCC	34	0.8500000000000001	No Hit
CCCAGATCACACACCCATTCACTGTACACAACTCTTCTTTGTTGCCCACA	31	0.775	No Hit
CGGCTAAGTAACCCCCCAGAAGAGTGAGCAGCCACAGCCTGCAGCCTCGG	21	0.525	No Hit
GTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAGAATCAG	19	0.475	No Hit
CCCGGCTAAGTAACCCCCCAGAAGAGTGAGCAGCCACAGCCTGCAGCCTC	17	0.42500000000000004	No Hit
CTCCCAAGTCTCATCAAGAGCAAACAGTGTAGGATGGGAGGATGGGAAAG	15	0.375	No Hit
GTCCGAAACGAGTCTTGGTGACTGAGCAGATTCCTTCTCAGCACCCAGGA	14	0.35000000000000003	No Hit
TCCCGGCTAAGTAACCCCCCAGAAGAGTGAGCAGCCACAGCCTGCAGCCT	14	0.35000000000000003	No Hit
GTCCGCAAAGGCTAGCTTTTCCCAAATGCCAACATCAGCTACCATAATAA	13	0.325	No Hit
TCCCCAGATCACACACCCATTCACTGTACACAACTCTTCTTTGTTGCCCA	13	0.325	No Hit
CCCCTCCACAAGCACAGAAACCTGTCGCAGGTCAGAAGTCAGTGCTAAAG	12	0.3	No Hit
CTCCTCTGATCCAAAAGTCTTTCCTGAGTCCTCCATGGTAAAATGGGCAA	12	0.3	No Hit
TCCGAAACGAGTCTTGGTGACTGAGCAGATTCCTTCTCAGCACCCAGGAT	12	0.3	No Hit
CTCCCAGCGTGTCCCTCCACAAGCACAGAAACCTGTCGCAGGTCAGAAGT	12	0.3	No Hit
CCCTCCACAAGCACAGAAACCTGTCGCAGGTCAGAAGTCAGTGCTAAAGC	12	0.3	No Hit
GGGCTGGTGGCGCAGCAAGTCCACAACATCTTTGGAGAACTGCTTTGTCC	12	0.3	No Hit
GGCTTGTTGTGAATCCACTTCACTACTTTGACGCCATTCTGAGCAGTGGC	12	0.3	No Hit
CCGGTTTTTCATGTCATTTTTTAACACTACAATTCTCTTATGACCATGTC	11	0.27499999999999997	No Hit
CCACTCAGCTGATTTACAATTTATGCATTTAATGCATAGTTTAAAAAATC	11	0.27499999999999997	No Hit
CCCCAGTGTGTGACCCACAGGTTTAAGGACATGTTTGGCTTCTTGAACAA	11	0.27499999999999997	No Hit
GCCAATTTGACTCTATTTTTGGGCTGCTTTTTTGCTGGGCTTTCCACTGC	11	0.27499999999999997	No Hit
GGCAAGGAATCTTCAGTGGTAACCTGGGGGGTAGATCGGAAATGAACTCT	10	0.25	No Hit
GTGTCTCCCTCACACCTGTGTTTTTCAGTGTGGGCACTGAAGCGGTTGGG	10	0.25	No Hit
CGGCACTGCTTTCCAAAATGGTGATGGTACAAAGGGATGAAATTACTGAT	10	0.25	No Hit
GCTGTTCACAGCACAGATTGCGTCATGCAGGTTTCTCATCTGCACGATGG	10	0.25	No Hit
GGGCAATGAGCACAGCAGCTTACTTTGGGAATAGCTGAGAGCTGTACTTT	10	0.25	No Hit
GGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGTGTAGAATCA	10	0.25	No Hit
CCCTCGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTTC	10	0.25	No Hit
CTGGGGCAGGTCATAGTGCTGAGAACTGGGAATCACTGGCTTCATTTAAA	10	0.25	No Hit
CACCACTCAGCTGATTTACAATTTATGCATTTAATGCATAGTTTAAAAAA	9	0.22499999999999998	No Hit
CCTGTGTCCTTCCAACTCCCAGCGTGTCCCTCCACAAGCACAGAAACCTG	9	0.22499999999999998	No Hit
GGTGGTAAGAGGGAATGAACTTGTTAAAGGGCATTCGGAAATGGGGCTGG	9	0.22499999999999998	No Hit
CTCTGGATAAAGAAATAGTGATTGGACTGGCCTCTTGGGCATGTCACGCT	9	0.22499999999999998	No Hit
GCTGCTTTTTTGCTGGGCTTTCCACTGCATCCTTGCTAATCAGTAGCCCG	9	0.22499999999999998	No Hit
GTGGCAATGTTCACTCCTGGAATGCAGGTGATGAAGTGCTCTAAGGGGAC	9	0.22499999999999998	No Hit
CTCACGGATTCTCTGTACAACAGTTGTTGCTGACCGGCCAGAGGGGACTG	9	0.22499999999999998	No Hit
CTCTTCTTTGTTGCCCACAGAACCTGACCTTTGAGATGACTCCAAGTAAC	9	0.22499999999999998	No Hit
ACAGACATAGTTACATAAAAAACAAGGCACACCAATAAGTCACCTAGAAG	9	0.22499999999999998	No Hit
GGGCAGGTCATAGTGCTGAGAACTGGGAATCACTGGCTTCATTTAAAAGA	9	0.22499999999999998	No Hit
CGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTTCTCTC	8	0.2	No Hit
GTCCCGGCTAAGTAACCCCCCAGAAGAGTGAGCAGCCACAGCCTGCAGCC	8	0.2	No Hit
TGTCCTTCCAACTCCCAGCGTGTCCCTCCACAAGCACAGAAACCTGTCGC	8	0.2	No Hit
CTTCCTTGTTCTCTTTACTGATGTCACACACACAGATAAACTCTGCTTCT	8	0.2	No Hit
CCCGGCACTGCTTTCCAAAATGGTGATGGTACAAAGGGATGAAATTACTG	8	0.2	No Hit
CCCCAGCGTGTCCCTCCACAAGCACAGAAACCTGTCGCAGGTCAGAAGTC	8	0.2	No Hit
CCCCAGAAGAGTGAGCAGCCACAGCCTGCAGCCTCGGGAAATTCTGAAAA	8	0.2	No Hit
GCCAGCGTTCCTCGACCTGTGTCCCGGCTAAGTAACCCCCCAGAAGAGTG	8	0.2	No Hit
GCTAACCAGCTGATCGGAAATGAACTCTTCAAATCTGATCAGCTGGTTAG	8	0.2	No Hit
CCTTGCTTCTTCTAGGGTATAGCTTGCCTCCTTATGTTGGGCTTTACCAT	8	0.2	No Hit
GCCGGTTTTTCATGTCATTTTTTAACACTACAATTCTCTTATGACCATGT	8	0.2	No Hit
CCGGCCAAAGTGGTGGTGGTAAGAGGGAATGAACTTGTTAAAGGGCATTC	8	0.2	No Hit
CCCAGCGTGTCCCTCCACAAGCACAGAAACCTGTCGCAGGTCAGAAGTCA	8	0.2	No Hit
CACAGGTGCAAAAGAAAAGTTGGCTGCCAATTTGACTCTATTTTTGGGCT	8	0.2	No Hit
CTGCTTTTTTGCTGGGCTTTCCACTGCATCCTTGCTAATCAGTAGCCCGG	8	0.2	No Hit
CTGGGTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCAC	8	0.2	No Hit
CCAGCGTTCCTCGACCTGTGTCCCGGCTAAGTAACCCCCCAGAAGAGTGA	8	0.2	No Hit
AACTGGAAGAAATAAAGTAAGAAAAAGTTCATCTGAAAGTTGGAAACAAA	7	0.17500000000000002	No Hit
ATGCCGGTGCCTGAGATCACTGAGTTCTAATGCAAAATTAACATCAGTTG	7	0.17500000000000002	No Hit
CTTCCAATTGAGTTTTCACATCTTGTGACATCTTGTAACAGCAATGGTTA	7	0.17500000000000002	No Hit
CTCGGGAAATTCTGAAAAGGAACAGACATCCATCCAGAAAACTGAAGACT	7	0.17500000000000002	No Hit
CCTGGCCATGAAATAAGAATCCATTTTCTGGCTTTCTAGAAAAAATAATA	7	0.17500000000000002	No Hit
CCGCAAAGGCTAGCTTTTCCCAAATGCCAACATCAGCTACCATAATAATA	7	0.17500000000000002	No Hit
CCCACACCATTTGAAGCACTTCACCACTCAGCTGATTTACAATTTATGCA	7	0.17500000000000002	No Hit
CCGGCTAAGTAACCCCCCAGAAGAGTGAGCAGCCACAGCCTGCAGCCTCG	7	0.17500000000000002	No Hit
GTGCAGGAGAGCTGGCAATGCTCTGGGAACTGAGGTTCTCTTTTTTTTGA	7	0.17500000000000002	No Hit
TTTGGCAATGTTGAAAGCAAGTGGCGAGGCGGTGTTCAAAAGGTTTGGAG	7	0.17500000000000002	No Hit
TTCCTGTCAACCGGAAAATGGCTCTCTTGCCACTCAATCATGCCCCAGTG	7	0.17500000000000002	No Hit
GTGGCGAGGCGGTGTTCAAAAGGTTTGGAGACATACTCCTCGGAGACCAA	7	0.17500000000000002	No Hit
TGCTGATGCTCTTGCCATCTTTATTTGCCGGCAGGTTATAAACATAGAGT	7	0.17500000000000002	No Hit
CGCTACTGGGATTGGCAATGTTGAAAGCAAGTGGCGAGGCGGTGTTCAAA	7	0.17500000000000002	No Hit
GGACTGTTGAAGATCTGACCTGAGACTGAGGCTCGGAAACATACCCTGCA	7	0.17500000000000002	No Hit
GGCAATGTTCACTCCTGGAATGCAGGTGATGAAGTGCTCTAAGGGGACAC	7	0.17500000000000002	No Hit
TTTTCCATTCGCTTCTGGGCCCGCTCTGGCTGTCTTGGTTTATAAAGCGG	7	0.17500000000000002	No Hit
CTGCCGGCCAAAGTGGTGGTGGTAAGAGGGAATGAACTTGTTAAAGGGCA	7	0.17500000000000002	No Hit
GGCTGGTGGCGCAGCAAGTCCACAACATCTTTGGAGAACTGCTTTGTCCT	6	0.15	No Hit
GCACCCAGGATCAGCGAGCAGTGGCCAGGCCCAGTGGGTCCTGTGTCCTT	6	0.15	No Hit
CCTGGCTTTCTAGAAAAAATAATATAAACATTTCCCTCGAAAGAAGTCAT	6	0.15	No Hit
CCTCGGGAAATTCTGAAAAGGAACAGACATCCATCCAGAAAACTGAAGAC	6	0.15	No Hit
ACCCAGGATCAGCGAGCAGTGGCCAGGCCCAGTGGGTCCTGTGTCCTTCC	6	0.15	No Hit
GTCTGCCTGAATATGGTCTTTTCTGTTTGGTTCAGTTTCTGTGTTGGTGT	6	0.15	No Hit
CTCTGATCCAAAAGTCTTTCCTGAGTCCTCCATGGTAAAATGGGCAAGAG	6	0.15	No Hit
CCTGGCAATGCTCTGGGAACTGAGGTTCTCTTTTTTTTGACTGCTTCTGT	6	0.15	No Hit
AGCCGGCCAAAGTGGTGGTGGTAAGAGGGAATGAACTTGTTAAAGGGCAT	6	0.15	No Hit
CGGCCAGAGGGGACTGAGCAGTTTTCAATATCCCAGAAAACACCAATGGG	6	0.15	No Hit
CTATGGACCATTAATAAAAGTGGCTTCTGGGGTCAAGACATGAAATAAGG	6	0.15	No Hit
CCTGTGGAGAGTGACACCAGTATCTTCTTGCTGCCAATTTTGTATCTGTG	6	0.15	No Hit
TGCTCTTAATTAAATGGAATCCCCAAATGGAAACTTTTTTAAAAAATGGG	6	0.15	No Hit
CTGCAGAACATTTCTAGAGCAGCTCCATCTGGGGCAGGTCATAGTGCTGA	6	0.15	No Hit
CCTCTGGATAAAGAAATAGTGATTGGACTGGCCTCTTGGGCATGTCACGC	6	0.15	No Hit
TGTCCTTTCTATTTCATCCTGAGTTCGTTCTCTTTTTCGGATACAAATGA	6	0.15	No Hit
GTGGGGGGGAAGGGGCAGCACAGCTTGTTTCTGTGGAGGCTGGACATCTC	6	0.15	No Hit
CTCCAATAGTTTGTTTCTTCCTCCTCTGATCCAAAAGTCTTTCCTGAGTC	6	0.15	No Hit
TGTGTGTTTTTCTGTGATTACCTCACTCAGGATGATATTTTCTAGTCCAA	6	0.15	No Hit
ATCCCGGCTAAGTAACCCCCCAGAAGAGTGAGCAGCCACAGCCTGCAGCC	6	0.15	No Hit
ACACCATTTGAAGCACTTCACCACTCAGCTGATTTACAATTTATGCATTT	6	0.15	No Hit
GGGGAAAGGTCAAATGAACTTAGCCGGTTTTTCATGTCATTTTTTAACAC	6	0.15	No Hit
ACTTCATGAAATTCGTAGGCAAATAAAATGAACTAGAAAATATCCTGAGT	6	0.15	No Hit
ATGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAG	6	0.15	No Hit
GCCATCATGTGACTGGGAAGAGCCCAGTGGGCTCTGGCGGGCCTGGTTAC	6	0.15	No Hit
CCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGTCAC	6	0.15	No Hit
CGGTCCTGTTCTGAGATGGAGCTCTGAGGCTAGATGGGGTTCTGGCACCT	6	0.15	No Hit
GCCGCCCACTAGGAAAAGGGAAGTTTGGAAATGTCTATTTGGCGAGGGAG	6	0.15	No Hit
CCGGCAGGTTATAAACATAGAGTAGAGTGTGGCAAGGAATCTTCAGTGGT	6	0.15	No Hit
AGGGCATTCGGAAATGGGGCTGGTGGCGCAGCAAGTCCACAACATCTTTG	6	0.15	No Hit
GCTGCAGTTTGTAGAGGAGCCATCATGTGACTGGGAAGAGCCCAGTGGGC	6	0.15	No Hit
CACGGCTTAGCATCGTTATTTTGGTGTAACCATCCAAGTGTTCTGCTGCA	6	0.15	No Hit
CCCTATTAAAAATGGGGTACAAAACTAAACAAACCATTCTCAGCTGAGGA	6	0.15	No Hit
GTCCTTTCTATTTCATCCTGAGTTCGTTCTCTTTTTCGGATACAAATGAC	6	0.15	No Hit
GACTGCTTCTGTGAAGTGTCTCCCTCACACCTGTGTTCTTCAGTGTGGGC	6	0.15	No Hit
CCTCCTCTGATCCAAAAGTCTTTCCTGAGTCCTCCATGGTAAAATGGGCA	6	0.15	No Hit
GCAGCCAGGCCTGCATAGAAAGTACCTTCAGGCATTTCACAAAGAGTGGC	6	0.15	No Hit
GCCGGCAGGTTATAAACATAGAGTAGAGTGTGGCAAGGAATCTTCAGTGG	6	0.15	No Hit
TTTTTTTTTGGGAGACTTTGCTTTATCAACAACAGCATTCGAACTCTTGG	6	0.15	No Hit
TCTGTGAAGTGCAGCAAAAATCCCAGTGTATTAGGATGTGTAAGATACCT	6	0.15	No Hit
TGCCTGGTTACTGGGTAAGAGACACACTAGTCGCCCATTTCCCTGTTCAC	6	0.15	No Hit
TTTCCCTCGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGG	6	0.15	No Hit
CCCCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGGT	6	0.15	No Hit
GGCAGGTCATAGTGCTGAGAACTGGGAATCACTGGCTTCATTTAAAAGAT	6	0.15	No Hit
GATAACTTTTGTCTCTTCAACAGATAAATCTAACTGGGTACATCCTTCAA	6	0.15	No Hit
GTCCCGGTTTCTGGTTTCAGCTTGCTGCAGCTGAGTCTCCAATTCAGCTT	6	0.15	No Hit
GCCTGGGACTTGAGAAGTTTCAGTAAATCCTGAGTAAAGCGCTTCACCTG	6	0.15	No Hit
CCTTCCTACCCTGACATTCTCCTACACTGGGGCATCAAACATTGGTAGCA	6	0.15	No Hit
CCCGGCGTGTCCCTCCACAAGCACAGAAACCTGTCGCAGGTCAGAAGTCA	6	0.15	No Hit
ACCATCTCTTTTCCTTAAACCTTACCCTTATCAAACGGAGACAGACAGAG	6	0.15	No Hit
CCTGAGATCACTGAGTTCTAATGCAAAATTAACATCAGTTGACACAAGAA	5	0.125	No Hit
CTCACCACTCAGCTGATTTACAATTTATGCATTTAATGCATAGTTTAAAA	5	0.125	No Hit
CCAATGATAAGCCTGTGAGAAATCGCTCACAATGACCTGCTTGCTGGCCT	5	0.125	No Hit
TTGGAAGATTTTGACATTGGCCGCCCACTAGGAAAAGGGAAGTTTGGAAA	5	0.125	No Hit
CTGGGCTTTCCACTGCATCCTTGCTAATCAGTAGCCCGGGGGCTTCTGAG	5	0.125	No Hit
CCAGTCCCATTTCCCAAGATCTTAAGCAATGTCATAACAAATCAGCTCCA	5	0.125	No Hit
GCAATGTTGAAAGCAAGTGGCGAGGCGGTGTTCAAAAGGTTTGGAGACAT	5	0.125	No Hit
GTTTGTTTCTTCCTCCTCTGATCCAAAAGTCTTTCCTGAGTCCTCCATGG	5	0.125	No Hit
AGGTAAGTAAGGTTGATTGTTACATTGATCTCTTAGAGGCAGGTAAGACA	5	0.125	No Hit
CACTGTAGCTATCTTCAGATACACCAGAAGAGGGCACTGGATCCCATTAC	5	0.125	No Hit
CTCCAGGATCTGCCTGCTGACTGCAGGCTGGTCTTCATGATTGACAGGGG	5	0.125	No Hit
GTTGGTTCTTTAGTAAATCAATGACTTCTCTGCTGAACTGAATCAGCTGA	5	0.125	No Hit
GTTCTCTCTGCTCTATGAATGGATTTGCCCTTTTTGCCCAACAGATACAA	5	0.125	No Hit
TCCCTCACTCTGCCCCACTAACTCAATAATAAGCTCCTGTCCTCCTGGGC	5	0.125	No Hit
GCTGGTGAGGACGAGCCTGGATGCTGCAAACCGAAGGTTGAGTGGCACCG	5	0.125	No Hit
TGCCAATTTTGTATCTGTGGAGGCTGTTCACAGCACAGATTGCGTCATGC	5	0.125	No Hit
GCGGTTATCAGTTGGATTTGTTTGCCAGACTCCATATCAGCAACCTTTAC	5	0.125	No Hit
CTTGGTCACAGGTGCAAAAGAAAAGTTGGCTGCCAATTTGACTCTATTTT	5	0.125	No Hit
CTTTAAATATTTTTCATTGATTCTATTGTTCAAATGTCCATTGGCTCATG	5	0.125	No Hit
CTGGCACCTCCAGGATCTGCCTGCTGACTGCAGGCTGGTCTTCATGATTG	5	0.125	No Hit
CCGGGTTATCAGTTGGATTTGTTTGCCAGACTCCATATCAGCAACCTTTA	5	0.125	No Hit
CCCAGTGGAGTCCTCAGTGTCATTACACTTTGTCTCATTCCCCGTGCCCA	5	0.125	No Hit
GCTTCCAACAGTGAACAGTAACAGTAAAGCTAGACACTATTAAAGACGTC	5	0.125	No Hit
AGTGTTTCTCCTACATATTTAGTATAGGCCATGGCAAATTCATGAAGAAA	5	0.125	No Hit
GTGGAGGCTGTTCACAGCACAGATTGCGTCATGCAGGTTTCTCATCTGCA	5	0.125	No Hit
GGGCTGCCAATTTGACTCTATTTTTGGGCTGCTTTTTTGCTGGGCTTTCC	5	0.125	No Hit
CTGTACTTAGTAAAGAGAGTGACTTGTTGGCAGCTCCTGTGGAGAGTGAC	5	0.125	No Hit
TCGCCAGGCCTGCATAGAAAGTACCTTCAGGCATTTCACAAAGAGTGGCA	5	0.125	No Hit
GAGCCATCATGTGACTGGGAAGAGCCCAGTGGGCTCTGGCGGGCCTGGTT	5	0.125	No Hit
AGTGATTCAAGGAACGGCCAATCAGAGCCTGCCCCACATGTGGCCCATAC	5	0.125	No Hit
CTCCACAAGCACAGAAACCTGTCGCAGGTCAGAAGTCAGTGCTAAAGCAG	5	0.125	No Hit
CTGCTGTTATGAGCTTTTCATTTCCAATTAACTTTCAGACATTAAGTCAA	5	0.125	No Hit
GCTGCCAATTTTGTATCTGTGGAGGCTGTTCACAGCACAGATTGCGTCAT	5	0.125	No Hit
GTGACATTTTTATCAAAATGCTGGCCATGAAATAAGAATCCATTTTCTGG	5	0.125	No Hit
ACCACCAGCAACTGAACAGTGAGAGGCCGCAAAGACTTCCGGGTTATCAG	5	0.125	No Hit
GTAGCTTATTTGGAAGACAGTCCCCTCTCTCTTTTTCTTCTGTGCAGGAG	5	0.125	No Hit
CTTCGCAGGCTCTGACGGAGTTTATCATCAGCAGCATTCTTTGCGGTAGC	5	0.125	No Hit
CCTCCTGAGTATTCTCTGCCCTCTGCTTGTAGGTCATGGCCAGGTCTCTC	5	0.125	No Hit
TTGCCGGCAGGTTATAAACATAGAGTAGAGTGTGGCAAGGAATCTTCAGT	5	0.125	No Hit
ACACCGTATTCCGTGACATCCCAGTCCTTTGAGAAACACCAATGATAAGC	5	0.125	No Hit
GTTCTGTGGGCAACAAAGAAGAGTTGTGTACAGTGAATGGGTGTGTGATC	5	0.125	No Hit
CATCTGACTAGTGGAAGACTGTGTGCCAACAGGTTCTCTCTGCTCTATGA	5	0.125	No Hit
GCTTGCTGACAGGACCCCATAGATGTCTCCTGAGAGGTTCTGCAAGTTCT	5	0.125	No Hit
GGTTCTCTCTGCTCTATGAATGGATTTGCCCTTTTTGCCCAACAGATACA	5	0.125	No Hit
GCAGGTCATAGTGCTGAGAACTGGGAATCACTGGCTTCATTTAAAAGATA	5	0.125	No Hit
TGCTCAATGGGACTCAATCCACTTCCTGCTGAACGATCTCTGCTTATTTC	5	0.125	No Hit
CTCAGCAAAGGCACAGTGCCCTCGTGGGTCTGCAGGAGACTATGAACTTG	5	0.125	No Hit
CACCCATTCACTGTACACAACTCTTCTTTGTTGCCCACAGAACCTGACCT	5	0.125	No Hit
CCCGAAAGAAGTCATCTGACTAGTGGAAGACTGTGTGCCAACAGGTTCTC	5	0.125	No Hit
CCCTTATCAAACGGAGACAGACAGAGAAGGGAAGCATGAAATAACTTTCT	5	0.125	No Hit
GCAGGCGGCTTCCTTTCATTTATTCCGTTTGGGAACTAAGAGGCAATGTC	5	0.125	No Hit
CCCCATTGCTGAAGACAGTGCCCACACAACCCAATGAACATGGAGAACTC	5	0.125	No Hit
GGCTTCTGGGGTCAAGACATGAAATAAGGTTTTTAAGCCTATTTTTGTGT	5	0.125	No Hit
CCCCCATCCTAATTTAATTCTGTGTTCAAACTGGAAGTTAGAGCTTGATC	5	0.125	No Hit
TCCCACACCATTTGAAGCACTTCACCACTCAGCTGATTTACAATTTATGC	5	0.125	No Hit
TCCAGGATCAGCGAGCAGTGGCCAGGCCCAGTGGGTCCTGTGTCCTTCCA	5	0.125	No Hit
TGGGTCCTGTGTCCTTCCAACTCCCAGCGTGTCCCTCCACAAGCACAGAA	5	0.125	No Hit
TGCTCATCAACGGGAAGATAAGCATCTCCTTCCCAAGACATCAACACCAC	5	0.125	No Hit
TGTGTGTAGAATCAGACAGTTTTCCCCCCATCCTAATTTAATTCTGTGTT	5	0.125	No Hit
CTGCAAAAGAGGCAACTCACACTCCTAACAGCAACTTTATCACTGCTTAG	5	0.125	No Hit
TCCCTGTTCACGGATTGCAATCATGTCTGTTAATTTATATAAATCAGACA	5	0.125	No Hit
AGTGTCCATACTTCTTTTCATAGATGTCTATAAATTTAAAGAGAGGCAAA	5	0.125	No Hit
CGGCCAAAGTGGTGGTGGTAAGAGGGAATGAACTTGTTAAAGGGCATTCG	5	0.125	No Hit
GGTTGCTGAAGAGAATGAATATCAGGAAGGGGAACTGCTGGGAAAAATTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.05	0.0	0.0	0.0
14-15	0.0	0.05	0.0	0.0	0.0
16-17	0.0	0.0875	0.0	0.0	0.0
18-19	0.0	0.125	0.0	0.0	0.0
20-21	0.0	0.125	0.0	0.0	0.0
22-23	0.0	0.125	0.0	0.0	0.0
24-25	0.0	0.125	0.0	0.0	0.0
26-27	0.0	0.125	0.0	0.0	0.0
28-29	0.0	0.125	0.0	0.0	0.0
30-31	0.0	0.125	0.0	0.0	0.0
32-33	0.0	0.125	0.0	0.0	0.0
34-35	0.0	0.15	0.0	0.0	0.0
36-37	0.0	0.3375	0.0	0.0	0.0
38-39	0.0	0.35	0.0	0.0	0.0
40-41	0.0	0.35	0.0	0.0	0.0
42-43	0.0	0.35	0.0	0.0	0.0
44-45	0.0	0.35	0.0	0.0	0.0
46-47	0.0	0.35	0.0	0.0	0.0
48-49	0.0	0.35	0.0	0.0	0.0
50-51	0.0	0.35	0.0	0.0	0.0
52-53	0.0	0.35	0.0	0.0	0.0
54-55	0.0	0.35	0.0	0.0	0.0
56-57	0.0	0.35	0.0	0.0	0.0
58-59	0.0	0.35	0.0	0.0	0.0
60-61	0.0	0.35	0.0	0.0	0.0
62-63	0.0	0.35	0.0	0.0	0.0
64-65	0.0	0.35	0.0	0.0	0.0
66-67	0.0	0.35	0.0	0.0	0.0
68-69	0.0	0.35	0.0	0.0	0.0
70-71	0.0	0.4375	0.0	0.0	0.0
72-73	0.0	0.525	0.0	0.0	0.0
74-75	0.0	0.525	0.0	0.0	0.0
76-77	0.0	0.525	0.0	0.0	0.0
78-79	0.0	0.525	0.0	0.0	0.0
80-81	0.0	0.525	0.0	0.0	0.0
82-83	0.0	0.575	0.0	0.0	0.0
84-85	0.0	0.6875	0.0	0.0	0.0
86-87	0.0	0.7	0.0	0.0	0.0
88-89	0.0	0.7	0.0	0.0	0.0
90-91	0.0	0.7	0.0	0.0	0.0
92-93	0.0	0.7	0.0	0.0	0.0
94-95	0.0	0.7	0.0	0.0	0.0
96-97	0.0	0.7	0.0	0.0	0.0
98-99	0.0	0.7	0.0	0.0	0.0
100-101	0.0	0.7	0.0	0.0	0.0
102-103	0.0	0.7	0.0	0.0	0.0
104-105	0.0	0.7	0.0	0.0	0.0
106-107	0.0	0.7	0.0	0.0	0.0
108-109	0.0	0.7	0.0	0.0	0.0
110-111	0.0	0.7	0.0	0.0	0.0
112-113	0.0	0.7	0.0	0.0	0.0
114-115	0.0	0.7	0.0	0.0	0.0
116-117	0.0	0.7	0.0	0.0	0.0
118-119	0.0	0.7	0.0	0.0	0.0
120-121	0.0	0.7	0.0	0.0	0.0
122-123	0.0	0.7	0.0	0.0	0.0
124-125	0.0	0.7	0.0	0.0	0.0
126-127	0.0	0.7	0.0	0.0	0.0
128-129	0.0	0.7	0.0	0.0	0.0
130-131	0.0	0.7	0.0	0.0	0.0
132-133	0.0	0.7	0.0	0.0	0.0
134-135	0.0	0.7	0.0	0.0	0.0
136-137	0.0	0.7	0.0	0.0	0.0
138-139	0.0	0.7	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCCTT	10	0.008592622	134.2875	1
>>END_MODULE
Read 7506264 spots for SRR26281813.sra
Written 7506264 spots for SRR26281813.sra
Read 7506264 spots for SRR26281813.sra
Written 7506264 spots for SRR26281813.sra
Read 7506264 spots for SRR26281813.sra
Written 7506264 spots for SRR26281813.sra
Read 7506264 spots for SRR26281813.sra
Written 7506264 spots for SRR26281813.sra
Read 7506264 spots for SRR26281813.sra
Written 7506264 spots for SRR26281813.sra
Read 7506264 spots for SRR26281813.sra
Written 7506264 spots for SRR26281813.sra
Read 7506264 spots for SRR26281813.sra
Written 7506264 spots for SRR26281813.sra
Rejected 14698 READS because READLEN < 1
Read 7506269 spots for SRR26281813.sra
Written 7506269 spots for SRR26281813.sra
SRR ids: ['SRR26281813.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_w2nlaga7
SRR26281813.sra spots: 60050117
blocks: [[1, 7506264], [7506265, 15012528], [15012529, 22518792], [22518793, 30025056], [30025057, 37531320], [37531321, 45037584], [45037585, 52543848], [52543849, 60050117]]
SRR26281813 file size 19124676
SRR26281813 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR26281813 SRR26281813_1.fastq SRR26281813_2.fastq
Input file:	SRR26281813_1.fastq
Paired file:	SRR26281813_2.fastq
trimmed:	SRR26281813-trimmed-pair1.fastq, SRR26281813-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Mon Nov 10 20:00:20 2025 >> started

Mon Nov 10 20:03:42 2025 >> done (201.186s)
60037912 read pairs processed; of these:
       0 ( 0.00%) short read pairs filtered out after trimming by size control
       0 ( 0.00%) empty read pairs filtered out after trimming by size control
60037912 (100.00%) read pairs available; of these:
  140958 ( 0.23%) trimmed read pairs available after processing
59896954 (99.77%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 35	    1068	  0.00%
 36	    1338	  0.00%
 37	    1552	  0.00%
 38	    1913	  0.00%
 39	    2372	  0.00%
 40	    2878	  0.00%
 41	    3326	  0.01%
 42	    3472	  0.01%
 43	    3376	  0.01%
 44	    3067	  0.01%
 45	    3184	  0.01%
 46	    3298	  0.01%
 47	    3992	  0.01%
 48	    4696	  0.01%
 49	    5468	  0.01%
 50	    6459	  0.01%
 51	    7310	  0.01%
 52	    7746	  0.01%
 53	    7707	  0.01%
 54	    7514	  0.01%
 55	    7546	  0.01%
 56	    7659	  0.01%
 57	    8718	  0.01%
 58	    9631	  0.02%
 59	   11384	  0.02%
 60	   12780	  0.02%
 61	   14046	  0.02%
 62	   15558	  0.03%
 63	   16566	  0.03%
 64	   17246	  0.03%
 65	   18053	  0.03%
 66	   17856	  0.03%
 67	   18462	  0.03%
 68	   19247	  0.03%
 69	   20797	  0.03%
 70	   23208	  0.04%
 71	   25603	  0.04%
 72	   29425	  0.05%
 73	   33516	  0.06%
 74	   37597	  0.06%
 75	   39808	  0.07%
 76	   41939	  0.07%
 77	   43003	  0.07%
 78	   43445	  0.07%
 79	   44491	  0.07%
 80	   47905	  0.08%
 81	   52461	  0.09%
 82	   59455	  0.10%
 83	   68412	  0.11%
 84	   77033	  0.13%
 85	   84668	  0.14%
 86	   89609	  0.15%
 87	   93113	  0.16%
 88	   95350	  0.16%
 89	   96616	  0.16%
 90	   99484	  0.17%
 91	  107098	  0.18%
 92	  117682	  0.20%
 93	  131634	  0.22%
 94	  145195	  0.24%
 95	  160756	  0.27%
 96	  172160	  0.29%
 97	  181052	  0.30%
 98	  183797	  0.31%
 99	  186218	  0.31%
100	  190232	  0.32%
101	  195715	  0.33%
102	  205565	  0.34%
103	  219610	  0.37%
104	  239480	  0.40%
105	  257877	  0.43%
106	  275464	  0.46%
107	  285960	  0.48%
108	  286583	  0.48%
109	  292823	  0.49%
110	  293231	  0.49%
111	  293857	  0.49%
112	  300605	  0.50%
113	  310283	  0.52%
114	  324721	  0.54%
115	  342504	  0.57%
116	  356663	  0.59%
117	  367965	  0.61%
118	  377306	  0.63%
119	  377355	  0.63%
120	  374980	  0.62%
121	  373693	  0.62%
122	  372791	  0.62%
123	  377305	  0.63%
124	  382676	  0.64%
125	  393686	  0.66%
126	  401037	  0.67%
127	  413384	  0.69%
128	  418384	  0.70%
129	  420727	  0.70%
130	  423448	  0.71%
131	  418376	  0.70%
132	  413947	  0.69%
133	  409912	  0.68%
134	  408761	  0.68%
135	  410712	  0.68%
136	  417884	  0.70%
137	  429386	  0.72%
138	  430112	  0.72%
139	  430323	  0.72%
140	  430407	  0.72%
141	  428034	  0.71%
142	  415133	  0.69%
143	  408174	  0.68%
144	  407805	  0.68%
145	  412582	  0.69%
146	  439324	  0.73%
147	  489929	  0.82%
148	  959952	  1.60%
149	 4380206	  7.30%
150	18425717	 30.69%
151	16016308	 26.68%
60037912 reads passed initial QC


criterion=sequence-density
sequence-density=0.62
sequence-density-rank=1
fanout-score=2.29
fanout-score-rank=40
prefix-density=0.65
prefix-fanout=2.2
sequence=CGAGACAAGACCAT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=36
fanout-score=252.67
fanout-score-rank=1
prefix-density=2.29
prefix-fanout=1.2
sequence=TCCTGCAGCCCTTCAGACCGCCAGAACTCTTCTGCTGCCTGCCCAACTGCCTGCCTGCCTGTGCCGAGAGTTCCCAGCACCATGAGGGCCTGGATCTTCTTTCTCCTTTGCCTGGCCGGGAGGGCCCTGGCAGCCCCTCAGACGGAAGCTGCAGAAGAGATGGTGGCGGAGGAAACCGTGGTGGAGGAGACAGGGTTACCTGTGGGTGCCAACCCAGTCCAGGTGGAAATGGGAGAGTTTGAAGAAGGTGCAGAGGAAACTGTCGAGGAGGTGGTGGCTGAAAACCCCTGCCAGAACCATCACTGCAAACATGGCAAGGTGTGTGAGCTGGACGAGAGCAACACCCCCATGTGTGTGTGCCAGGACCCCACCAGCTGCCCAGCTCCCATTGGCGAGTTTGAAAAGGTGTGCAGCAATGACAACAAGACCTTCGACTCTTCCTGCCACTTCTTTGCGACCAAGTGCACCCTGGAGGGCACCAAGAAGGGCCACAAGCTCCACCTGGACTACA


criterion=sequence-density
sequence-density=0.71
sequence-density-rank=1
fanout-score=4.13
fanout-score-rank=30
prefix-density=0.96
prefix-fanout=3.0
sequence=ATGGTCTTGTCTCG


criterion=fanout-score
sequence-density=0.04
sequence-density-rank=29
fanout-score=90.71
fanout-score-rank=1
prefix-density=0.27
prefix-fanout=12.3
sequence=CCACCAGCTGGGAAGGATGTGTCAGTGGAATGGATGCAGCTAATCTTTCTTCAAGTCCCTGGATTCAAAGAGCTTCAAGCGCTCCTGCACCGTCAGGCCTTCCTTCAGACTACGACAGGGGACTTGGAAGCGGGCGCGACTCAGTTATCCCATGAGACCTGCTCAATTACGGACTTGGACCTGATGTTTCTGAGTTGCCGGGTAACACAGGATCTGTCTTTCTTCAGGAAGTCAGGATTGCTTTTAGATTTCATTATGTCATATCCTGACACAGTAGTGCCTTCAGGAGATTTCTCGCCCAGTGCCTCT
SRR26281813 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Nov 10 20:04:08
                             Started mapping on |	Nov 10 20:04:08
                                    Finished on |	Nov 10 20:11:51
       Mapping speed, Million of reads per hour |	466.82

                          Number of input reads |	60037912
                      Average input read length |	280
                                    UNIQUE READS:
                   Uniquely mapped reads number |	54882194
                        Uniquely mapped reads % |	91.41%
                          Average mapped length |	278.86
                       Number of splices: Total |	63233508
            Number of splices: Annotated (sjdb) |	61493374
                       Number of splices: GT/AG |	62279999
                       Number of splices: GC/AG |	391801
                       Number of splices: AT/AC |	43364
               Number of splices: Non-canonical |	518344
                      Mismatch rate per base, % |	0.44%
                         Deletion rate per base |	0.03%
                        Deletion average length |	1.65
                        Insertion rate per base |	0.01%
                       Insertion average length |	1.66
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	5146050
             % of reads mapped to multiple loci |	8.57%
        Number of reads mapped to too many loci |	4521
             % of reads mapped to too many loci |	0.01%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.01%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	9685	9685	9685
N_multimapping	5146050	5146050	5146050
N_noFeature	5937678	6724523	53666337
N_ambiguous	834095	399710	8588
UnstrandedReadsAssigned:48110421 PositiveStrandReadsAssigned:47757961 NegativeStrandReadsAssigned:1207269
Dataset is classified positive stranded
MeadianReadLen=150 20thPercentileLength=128 echo kmer=123
SRR26281813 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 31,715
[index] number of k-mers: 49,915,185
[index] number of equivalence classes: 70,351
[quant] running in paired-end mode
[quant] will process pair 1: SRR26281813-trimmed-pair1.fastq
                             SRR26281813-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 60,037,912 reads, 51,095,727 reads pseudoaligned
[quant] estimated average fragment length: 194.408
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,086 rounds

  31716 SRR26281813.ke.tsv
  32883 SRR26281813.se.tsv
  64599 total
==> SRR26281813.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	761.592	90732	3611.79
ENSRNOT00000040993.4	1039	845.592	123993	4445.51
ENSRNOT00000050156.3	1545	1351.59	666215	14943.5
ENSRNOT00000043693.3	684	490.867	165516	10222.6
ENSRNOT00000046201.3	204	58.8144	41302	21289.8
ENSRNOT00000046108.3	681	487.948	215459	13386.8
ENSRNOT00000049683.3	784	590.673	229171	11762.4
ENSRNOT00000041241.3	348	168.903	16943	3041.15
ENSRNOT00000044582.3	297	126.369	16819.2	4035.07

==> SRR26281813.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	21
ENSRNOG00000058808	0
ENSRNOG00000061316	1305
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	7
SRR26281813 completed mapping pipeline successfully
