Starting /dee2/code/volunteer_pipeline.sh SRR32454945
    current disk space = 1213982580
    free memory = 194230024 
SRR32454945 SRAfilesize
beedbc5bba531e3138da4db4a1ccb7a9  SRR32454945.sra
SRR32454945.sra file validated
SRR32454945 is single end
SRR32454945 is conventional basespace
SRR32454945 read1 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32454945_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.0955	37.0	37.0	37.0	37.0	37.0
2	36.272	37.0	37.0	37.0	37.0	37.0
3	36.334	37.0	37.0	37.0	37.0	37.0
4	36.527	37.0	37.0	37.0	37.0	37.0
5	36.431	37.0	37.0	37.0	37.0	37.0
6	36.4035	37.0	37.0	37.0	37.0	37.0
7	36.5125	37.0	37.0	37.0	37.0	37.0
8	36.533	37.0	37.0	37.0	37.0	37.0
9	36.248	37.0	37.0	37.0	37.0	37.0
10	36.5375	37.0	37.0	37.0	37.0	37.0
11	36.526	37.0	37.0	37.0	37.0	37.0
12	36.4865	37.0	37.0	37.0	37.0	37.0
13	36.517	37.0	37.0	37.0	37.0	37.0
14	36.677	37.0	37.0	37.0	37.0	37.0
15	36.419	37.0	37.0	37.0	37.0	37.0
16	36.42	37.0	37.0	37.0	37.0	37.0
17	36.4425	37.0	37.0	37.0	37.0	37.0
18	36.1995	37.0	37.0	37.0	37.0	37.0
19	36.4135	37.0	37.0	37.0	37.0	37.0
20	36.277	37.0	37.0	37.0	37.0	37.0
21	36.3115	37.0	37.0	37.0	37.0	37.0
22	36.593	37.0	37.0	37.0	37.0	37.0
23	36.471	37.0	37.0	37.0	37.0	37.0
24	36.562	37.0	37.0	37.0	37.0	37.0
25	36.4725	37.0	37.0	37.0	37.0	37.0
26	36.3815	37.0	37.0	37.0	37.0	37.0
27	36.4935	37.0	37.0	37.0	37.0	37.0
28	36.3575	37.0	37.0	37.0	37.0	37.0
29	36.383	37.0	37.0	37.0	37.0	37.0
30	36.342	37.0	37.0	37.0	37.0	37.0
31	36.5685	37.0	37.0	37.0	37.0	37.0
32	36.353	37.0	37.0	37.0	37.0	37.0
33	36.3385	37.0	37.0	37.0	37.0	37.0
34	36.3355	37.0	37.0	37.0	37.0	37.0
35	36.083	37.0	37.0	37.0	37.0	37.0
36	35.7585	37.0	37.0	37.0	37.0	37.0
37	36.301	37.0	37.0	37.0	37.0	37.0
38	36.377	37.0	37.0	37.0	37.0	37.0
39	36.433	37.0	37.0	37.0	37.0	37.0
40	36.128	37.0	37.0	37.0	37.0	37.0
41	36.2895	37.0	37.0	37.0	37.0	37.0
42	36.2235	37.0	37.0	37.0	37.0	37.0
43	36.226	37.0	37.0	37.0	37.0	37.0
44	36.317	37.0	37.0	37.0	37.0	37.0
45	36.2565	37.0	37.0	37.0	37.0	37.0
46	36.3005	37.0	37.0	37.0	37.0	37.0
47	36.237	37.0	37.0	37.0	37.0	37.0
48	36.285	37.0	37.0	37.0	37.0	37.0
49	36.2715	37.0	37.0	37.0	37.0	37.0
50	36.2115	37.0	37.0	37.0	37.0	37.0
51	35.885	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	4.0
25	1.0
26	9.0
27	15.0
28	17.0
29	31.0
30	24.0
31	39.0
32	47.0
33	63.0
34	95.0
35	179.0
36	1550.0
37	1926.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	65.44817225838759	16.775162744116173	9.839759639459189	7.936905358037055
2	20.200000000000003	8.3	6.75	64.75
3	16.425	59.025000000000006	4.2250000000000005	20.325
4	18.224999999999998	5.45	61.1	15.225
5	44.1	6.0	35.9	14.000000000000002
6	69.625	4.3999999999999995	12.35	13.625000000000002
7	69.875	8.175	10.075000000000001	11.875
8	17.25	8.025	66.07499999999999	8.649999999999999
9	70.55	6.9750000000000005	10.8	11.675
10	71.525	7.5249999999999995	11.95	9.0
11	17.0	4.175	62.824999999999996	16.0
12	14.174999999999999	10.225	65.225	10.375
13	12.975	8.75	17.25	61.025
14	20.375	64.325	10.549999999999999	4.75
15	75.225	8.575000000000001	12.425	3.775
16	16.125	11.825	66.8	5.25
17	68.875	10.174999999999999	15.950000000000001	5.0
18	66.45	10.95	16.375	6.225
19	13.15	10.299999999999999	70.275	6.275
20	9.475	62.55	20.525	7.449999999999999
21	63.3	15.225	12.85	8.625
22	16.55	59.875	15.9	7.675
23	15.375	58.050000000000004	17.75	8.825
24	16.825000000000003	8.5	65.675	9.0
25	16.6	2.8000000000000003	68.75	11.85
26	16.525000000000002	4.9750000000000005	14.625	63.87500000000001
27	16.475	5.475	62.25000000000001	15.8
28	20.525	5.525	9.700000000000001	64.25
29	67.80000000000001	8.200000000000001	7.9750000000000005	16.025
30	16.2	8.625	8.6	66.57499999999999
31	17.775	10.725	27.6	43.9
32	36.75	12.4	40.1	10.75
33	68.85	14.825	7.324999999999999	9.0
34	52.825	35.099999999999994	5.1499999999999995	6.925000000000001
35	14.374999999999998	69.75	7.875	8.0
36	9.575	50.724999999999994	29.549999999999997	10.15
37	9.85	13.700000000000001	61.925	14.524999999999999
38	7.575	10.775	46.75	34.9
39	6.175	11.700000000000001	30.25	51.87500000000001
40	9.925	10.95	41.4	37.724999999999994
41	30.3	11.175	10.2	48.325
42	67.55	10.15	7.75	14.549999999999999
43	67.375	10.25	8.95	13.425
44	46.675	11.65	28.249999999999996	13.425
45	30.325000000000003	14.35	40.925	14.399999999999999
46	42.55	11.85	10.65	34.949999999999996
47	8.55	14.75	10.299999999999999	66.4
48	8.15	35.275	8.7	47.875
49	11.3	68.375	9.65	10.674999999999999
50	32.275	51.275000000000006	8.275	8.175
51	64.525	19.725	10.7	5.050000000000001
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.5
33	1.0
34	0.5
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	1.0
43	2.0
44	6.0
45	10.0
46	39.5
47	69.0
48	73.0
49	77.0
50	187.0
51	297.0
52	853.0
53	1409.0
54	1385.0
55	1361.0
56	927.0
57	493.0
58	328.0
59	163.0
60	116.0
61	69.0
62	45.5
63	22.0
64	18.5
65	15.0
66	11.5
67	8.0
68	6.0
69	4.0
70	2.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.1
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.75324675324676	16.575
2	13.41991341991342	6.2
3	4.545454545454546	3.15
4	1.6233766233766231	1.5
5	1.83982683982684	2.125
6	1.1904761904761905	1.6500000000000001
7	0.4329004329004329	0.7000000000000001
8	0.6493506493506493	1.2
9	0.21645021645021645	0.44999999999999996
>10	3.896103896103896	20.349999999999998
>50	0.0	0.0
>100	0.21645021645021645	16.875
>500	0.21645021645021645	29.225
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG	653	16.325	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG	516	12.9	No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG	339	8.475000000000001	Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG	336	8.4	Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAT	50	1.25	RNA PCR Primer, Index 1 (97% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG	43	1.075	No Hit
ATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGGA	41	1.0250000000000001	RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGG	41	1.0250000000000001	Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA	40	1.0	No Hit
GCATGGGTTGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG	37	0.9249999999999999	No Hit
GCATTGGTTGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG	36	0.8999999999999999	No Hit
AGGGCTGGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGATGA	32	0.8	RNA PCR Primer, Index 1 (97% over 37bp)
GACTCTTAGCGGTGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA	31	0.775	RNA PCR Primer, Index 1 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	29	0.7250000000000001	RNA PCR Primer, Index 1 (100% over 32bp)
AGGGCAAAAGCTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT	26	0.65	RNA PCR Primer, Index 1 (100% over 35bp)
AGGGCTGGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGATG	25	0.625	RNA PCR Primer, Index 1 (97% over 37bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGG	24	0.6	Illumina Small RNA Adapter 2 (100% over 21bp)
AGAAGCCCGGCTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA	23	0.575	RNA PCR Primer, Index 1 (97% over 37bp)
GTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA	21	0.525	RNA PCR Primer, Index 1 (97% over 37bp)
GACTCTTAGCGGTGGATCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	21	0.525	RNA PCR Primer, Index 1 (100% over 30bp)
AGAAGCCCGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAT	20	0.5	RNA PCR Primer, Index 1 (97% over 37bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAAG	19	0.475	No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA	19	0.475	RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGATGGAATTCTCGGGTGCCAAG	18	0.44999999999999996	No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAG	18	0.44999999999999996	No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA	17	0.42500000000000004	RNA PCR Primer, Index 1 (100% over 28bp)
CGGCGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGATGATC	17	0.42500000000000004	RNA PCR Primer, Index 1 (97% over 37bp)
AGGGCAAAAGCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG	17	0.42500000000000004	RNA PCR Primer, Index 15 (100% over 36bp)
AGCGCCCGTCCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAT	15	0.375	RNA PCR Primer, Index 1 (97% over 37bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	15	0.375	RNA PCR Primer, Index 1 (100% over 29bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGGA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTTGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG	14	0.35000000000000003	Illumina Small RNA Adapter 2 (100% over 21bp)
AGAAGCCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGATGA	13	0.325	RNA PCR Primer, Index 1 (97% over 37bp)
ATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAA	13	0.325	RNA PCR Primer, Index 1 (100% over 23bp)
AAGGGCAAAAGCTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA	12	0.3	RNA PCR Primer, Index 1 (100% over 34bp)
GGGAGCCTGAGAAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA	11	0.27499999999999997	RNA PCR Primer, Index 1 (97% over 37bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG	11	0.27499999999999997	Illumina Small RNA Adapter 2 (100% over 21bp)
GGGCTGGAGAGATGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 34bp)
CGCGACCTCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGATG	10	0.25	RNA PCR Primer, Index 1 (97% over 37bp)
GCATGGGTTGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG	10	0.25	Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG	9	0.22499999999999998	Illumina Small RNA Adapter 2 (100% over 21bp)
AGAAGCCCGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGATG	9	0.22499999999999998	RNA PCR Primer, Index 1 (97% over 37bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG	8	0.2	RNA PCR Primer, Index 15 (100% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA	8	0.2	No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCAA	8	0.2	No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA	8	0.2	No Hit
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT	8	0.2	RNA PCR Primer, Index 1 (100% over 35bp)
GACTCTTAGCGGTGGATCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	8	0.2	RNA PCR Primer, Index 1 (100% over 31bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA	7	0.17500000000000002	RNA PCR Primer, Index 1 (97% over 37bp)
AGCAGGACGGTGGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 35bp)
AGCCCGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGATGAT	7	0.17500000000000002	RNA PCR Primer, Index 1 (97% over 37bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG	7	0.17500000000000002	No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGGA	6	0.15	RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAGGA	6	0.15	RNA PCR Primer, Index 1 (100% over 22bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTTGGAATTCTCGGGTGCC	6	0.15	No Hit
GACTCTTAGCGGTGGATCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	6	0.15	RNA PCR Primer, Index 1 (100% over 32bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
GGGCTGGAGAGATGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT	6	0.15	RNA PCR Primer, Index 1 (100% over 35bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
AGCGCCCGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGATGA	6	0.15	RNA PCR Primer, Index 1 (97% over 37bp)
TTCCGGAGAGGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG	6	0.15	RNA PCR Primer, Index 15 (100% over 36bp)
GTTTCCGTAGTGTAGTGGTTATCACGCTCGCCTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
AGAAGCCGGCGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA	5	0.125	RNA PCR Primer, Index 1 (100% over 34bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGA	5	0.125	RNA PCR Primer, Index 1 (100% over 22bp)
AAGGGGGCCGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAT	5	0.125	RNA PCR Primer, Index 1 (97% over 37bp)
ACTCTTAGCGGTGGATCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	5	0.125	RNA PCR Primer, Index 1 (100% over 32bp)
GCATGAGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG	5	0.125	No Hit
GGGTCTGTAGCTCAGGTGGTTAGAGCACCGTCTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA	5	0.125	No Hit
AAGCCCGGCTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGATG	5	0.125	RNA PCR Primer, Index 1 (97% over 37bp)
AAAGGGGGGCGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA	5	0.125	RNA PCR Primer, Index 1 (97% over 37bp)
CGCGACCTCAGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA	5	0.125	RNA PCR Primer, Index 1 (97% over 37bp)
AGGGGGCCGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAT	5	0.125	RNA PCR Primer, Index 1 (97% over 37bp)
AAGCCCGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGATGA	5	0.125	RNA PCR Primer, Index 1 (97% over 37bp)
TTCCGGAGAGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT	5	0.125	RNA PCR Primer, Index 1 (100% over 35bp)
CCCAGTGTTCAGACTACCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGGA	5	0.125	RNA PCR Primer, Index 1 (100% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.025	0.0	0.0	0.0
4	0.0	0.075	0.0	0.0	0.0
5	0.0	0.075	0.0	0.0	0.0
6	0.0	0.15	0.0	0.0	0.0
7	0.0	0.325	0.0	0.0	0.0
8	0.0	0.375	0.0	0.0	0.0
9	0.0	0.8	0.0	0.0	0.0
10	0.0	2.25	0.0	0.0	0.0
11	0.0	4.05	0.0	0.0	0.0
12	0.0	7.025	0.0	0.0	0.0
13	0.0	9.925	0.0	0.0	0.0
14	0.0	14.1	0.0	0.0	0.0
15	0.0	17.675	0.0	0.0	0.0
16	0.0	20.075	0.0	0.0	0.0
17	0.0	22.725	0.0	0.0	0.0
18	0.0	25.6	0.0	0.0	0.0
19	0.0	26.275	0.0	0.0	0.0
20	0.0	27.85	0.0	0.0	0.0
21	0.0	28.675	0.0	0.0	0.0
22	0.0	29.975	0.0	0.0	0.0
23	0.0	31.875	0.0	0.0	0.0
24	0.0	33.05	0.0	0.0	0.0
25	0.0	33.475	0.0	0.0	0.0
26	0.0	33.9	0.0	0.0	0.0
27	0.0	34.15	0.0	0.0	0.0
28	0.0	34.4	0.0	0.0	0.0
29	0.0	35.275	0.0	0.0	0.0
30	0.0	38.1	0.0	0.0	0.0
31	0.0	58.7	0.0	0.0	0.0
32	0.0	93.975	0.0	0.0	0.0
33	0.0	96.875	0.0	0.0	0.0
34	0.0	97.475	0.0	0.0	0.0
35	0.0	97.925	0.0	0.0	0.0
36	0.0	98.175	0.0	0.0	0.0
37	0.0	98.225	0.0	0.0	0.0
38	0.0	98.225	0.0	0.0	0.0
39	0.0	98.225	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATTGG	65	0.0	45.000004	1
GGTTGTT	20	6.222983E-4	45.0	6
TTGTTCA	20	6.222983E-4	45.0	8
ATTGGTG	55	0.0	45.0	3
GCATGGG	140	0.0	45.0	1
TTGGTGG	55	0.0	45.0	4
CATGGGT	140	0.0	45.0	2
TGGTGGT	55	0.0	45.0	5
GTTGTTC	20	6.222983E-4	45.0	7
AGTGGTA	210	0.0	43.92857	14
GTTCAGT	210	0.0	43.92857	10
GTGGTAG	210	0.0	43.92857	15
TTCAGTG	210	0.0	43.92857	11
CAGTGGT	210	0.0	43.92857	13
GGTTCAG	190	0.0	43.815792	9
GGTGGTT	190	0.0	43.815792	6
ATGGGTG	135	0.0	43.333336	3
TGGGTGG	135	0.0	43.333336	4
GGGTGGT	135	0.0	43.333336	5
TCTCGCC	120	0.0	43.124996	25
>>END_MODULE
Read 3830896 spots for SRR32454945.sra
Written 3830896 spots for SRR32454945.sra
Read 3830896 spots for SRR32454945.sra
Written 3830896 spots for SRR32454945.sra
Read 3830896 spots for SRR32454945.sra
Written 3830896 spots for SRR32454945.sra
Read 3830896 spots for SRR32454945.sra
Written 3830896 spots for SRR32454945.sra
Read 3830899 spots for SRR32454945.sra
Written 3830899 spots for SRR32454945.sra
Read 3830896 spots for SRR32454945.sra
Written 3830896 spots for SRR32454945.sra
Read 3830896 spots for SRR32454945.sra
Written 3830896 spots for SRR32454945.sra
Read 3830896 spots for SRR32454945.sra
Written 3830896 spots for SRR32454945.sra
SRR ids: ['SRR32454945.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_o8jd5b07
SRR32454945.sra spots: 30647171
blocks: [[1, 3830896], [3830897, 7661792], [7661793, 11492688], [11492689, 15323584], [15323585, 19154480], [19154481, 22985376], [22985377, 26816272], [26816273, 30647171]]
SRR32454945 file size 5270664
SRR32454945 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32454945 SRR32454945_1.fastq
Input file:	SRR32454945_1.fastq
trimmed:	SRR32454945-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Sat Nov  1 20:02:18 2025 >> started

Sat Nov  1 20:02:28 2025 >> done (9.868s)
30647171 reads processed; of these:
     137 ( 0.00%) short reads filtered out after trimming by size control
       0 ( 0.00%) empty reads filtered out after trimming by size control
30647034 (100.00%) reads available; of these:
     339 ( 0.00%) trimmed reads available after processing
30646695 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 50	     339	  0.00%
 51	30646695	100.00%
30647034 reads passed initial QC


criterion=sequence-density
sequence-density=69.59
sequence-density-rank=1
fanout-score=3.01
fanout-score-rank=6
prefix-density=69.83
prefix-fanout=3.0
sequence=CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGATGATCTCGTATGCC


criterion=fanout-score
sequence-density=2.58
sequence-density-rank=8
fanout-score=21.56
fanout-score-rank=1
prefix-density=2.55
prefix-fanout=21.6
sequence=GGTGGAATTCTC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGATGATCTCGTATGCC -o SRR32454945 -
Input file:	STDIN
trimmed:	SRR32454945-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGATGATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Sat Nov  1 20:03:46 2025 >> started

Sat Nov  1 20:04:09 2025 >> done (22.730s)
29771405 reads processed; of these:
 7851252 (26.37%) short reads filtered out after trimming by size control
   16423 ( 0.06%) empty reads filtered out after trimming by size control
21903730 (73.57%) reads available; of these:
21742159 (99.26%) trimmed reads available after processing
  161571 ( 0.74%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  491289	  2.24%
 19	  354783	  1.62%
 20	  453891	  2.07%
 21	  524517	  2.39%
 22	  366395	  1.67%
 23	  157879	  0.72%
 24	  123932	  0.57%
 25	   90549	  0.41%
 26	   91403	  0.42%
 27	  337944	  1.54%
 28	  872037	  3.98%
 29	 5728185	 26.15%
 30	10621227	 48.49%
 31	 1059111	  4.84%
 32	  202703	  0.93%
 33	  165300	  0.75%
 34	   67682	  0.31%
 35	   15500	  0.07%
 36	    8680	  0.04%
 37	    2639	  0.01%
 38	    1499	  0.01%
 39	    1214	  0.01%
 40	    1041	  0.00%
 41	     370	  0.00%
 42	     352	  0.00%
 43	     356	  0.00%
 44	     210	  0.00%
 45	     222	  0.00%
 46	     465	  0.00%
 47	     225	  0.00%
 48	     559	  0.00%
 49	       0	  0.00%
 50	      31	  0.00%
 51	  161540	  0.74%


criterion=sequence-density
sequence-density=67.70
sequence-density-rank=1
fanout-score=1.99
fanout-score-rank=7
prefix-density=67.55
prefix-fanout=2.0
sequence=GGTGGTTCAGTGGTAGAATTCTCGCCT


criterion=fanout-score
sequence-density=0.13
sequence-density-rank=29
fanout-score=122.00
fanout-score-rank=1
prefix-density=7.21
prefix-fanout=2.2
sequence=TGGTCTAGTGGCTAGGATTCGGC
                                 Started job on |	Nov 01 20:04:46
                             Started mapping on |	Nov 01 20:04:46
                                    Finished on |	Nov 01 20:05:50
       Mapping speed, Million of reads per hour |	1281.34

                          Number of input reads |	22779359
                      Average input read length |	29
                                    UNIQUE READS:
                   Uniquely mapped reads number |	855812
                        Uniquely mapped reads % |	3.76%
                          Average mapped length |	22.02
                       Number of splices: Total |	6704
            Number of splices: Annotated (sjdb) |	1089
                       Number of splices: GT/AG |	6342
                       Number of splices: GC/AG |	128
                       Number of splices: AT/AC |	1
               Number of splices: Non-canonical |	233
                      Mismatch rate per base, % |	0.49%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.36
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.17
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	10963222
             % of reads mapped to multiple loci |	48.13%
        Number of reads mapped to too many loci |	9805472
             % of reads mapped to too many loci |	43.05%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	5.04%
                     % of reads unmapped: other |	0.03%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	10960325	10960325	10960325
N_multimapping	10963222	10963222	10963222
N_noFeature	150325	186061	816874
N_ambiguous	55472	52252	24
UnstrandedReadsAssigned:650015 PositiveStrandReadsAssigned:617499 NegativeStrandReadsAssigned:38914
Dataset is classified positive stranded
MeadianReadLen=30 20thPercentileLength=29 echo kmer=25
SRR32454945 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=25

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 25
[index] number of targets: 31,715
[index] number of k-mers: 49,762,319
[index] number of equivalence classes: 84,429
[quant] running in single-end mode
[quant] will process file 1: SRR32454945-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 22,779,359 reads, 7,670 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 581 rounds

  31716 SRR32454945.ke.tsv
  32883 SRR32454945.se.tsv
  64599 total
==> SRR32454945.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	856	1	82.538
ENSRNOT00000040993.4	1039	940	0	0
ENSRNOT00000050156.3	1545	1446	9	439.746
ENSRNOT00000043693.3	684	585	2	241.547
ENSRNOT00000046201.3	204	105	0	0
ENSRNOT00000046108.3	681	582	1	121.396
ENSRNOT00000049683.3	784	685	0	0
ENSRNOT00000041241.3	348	249	0	0
ENSRNOT00000044582.3	297	198	0	0

==> SRR32454945.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	0
ENSRNOG00000058808	0
ENSRNOG00000061316	0
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	0
SRR32454945 completed mapping pipeline successfully
