Starting /dee2/code/volunteer_pipeline.sh SRR32454947
    current disk space = 1101471396
    free memory = 197348820 
SRR32454947 SRAfilesize
d053dcc318a5f9a642126c9a46caa6bd  SRR32454947.sra
SRR32454947.sra file validated
SRR32454947 is single end
SRR32454947 is conventional basespace
SRR32454947 read1 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32454947_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.265	37.0	37.0	37.0	37.0	37.0
2	36.183	37.0	37.0	37.0	37.0	37.0
3	36.3415	37.0	37.0	37.0	37.0	37.0
4	36.1285	37.0	37.0	37.0	37.0	37.0
5	36.3215	37.0	37.0	37.0	37.0	37.0
6	36.4055	37.0	37.0	37.0	37.0	37.0
7	36.4435	37.0	37.0	37.0	37.0	37.0
8	36.479	37.0	37.0	37.0	37.0	37.0
9	35.8885	37.0	37.0	37.0	37.0	37.0
10	36.426	37.0	37.0	37.0	37.0	37.0
11	36.475	37.0	37.0	37.0	37.0	37.0
12	36.438	37.0	37.0	37.0	37.0	37.0
13	36.451	37.0	37.0	37.0	37.0	37.0
14	36.677	37.0	37.0	37.0	37.0	37.0
15	36.3735	37.0	37.0	37.0	37.0	37.0
16	36.4305	37.0	37.0	37.0	37.0	37.0
17	36.3695	37.0	37.0	37.0	37.0	37.0
18	36.3085	37.0	37.0	37.0	37.0	37.0
19	36.426	37.0	37.0	37.0	37.0	37.0
20	36.3305	37.0	37.0	37.0	37.0	37.0
21	36.3615	37.0	37.0	37.0	37.0	37.0
22	36.432	37.0	37.0	37.0	37.0	37.0
23	36.241	37.0	37.0	37.0	37.0	37.0
24	36.2925	37.0	37.0	37.0	37.0	37.0
25	36.3325	37.0	37.0	37.0	37.0	37.0
26	36.2605	37.0	37.0	37.0	37.0	37.0
27	36.1785	37.0	37.0	37.0	37.0	37.0
28	36.252	37.0	37.0	37.0	37.0	37.0
29	36.222	37.0	37.0	37.0	37.0	37.0
30	36.2925	37.0	37.0	37.0	37.0	37.0
31	36.2945	37.0	37.0	37.0	37.0	37.0
32	36.219	37.0	37.0	37.0	37.0	37.0
33	36.282	37.0	37.0	37.0	37.0	37.0
34	36.21	37.0	37.0	37.0	37.0	37.0
35	36.129	37.0	37.0	37.0	37.0	37.0
36	35.7445	37.0	37.0	37.0	37.0	37.0
37	36.202	37.0	37.0	37.0	37.0	37.0
38	36.1955	37.0	37.0	37.0	37.0	37.0
39	36.1695	37.0	37.0	37.0	37.0	37.0
40	36.19	37.0	37.0	37.0	37.0	37.0
41	36.204	37.0	37.0	37.0	37.0	37.0
42	36.1355	37.0	37.0	37.0	37.0	37.0
43	35.989	37.0	37.0	37.0	37.0	37.0
44	36.219	37.0	37.0	37.0	37.0	37.0
45	36.2685	37.0	37.0	37.0	37.0	37.0
46	36.2585	37.0	37.0	37.0	37.0	37.0
47	36.167	37.0	37.0	37.0	37.0	37.0
48	36.203	37.0	37.0	37.0	37.0	37.0
49	36.296	37.0	37.0	37.0	37.0	37.0
50	36.223	37.0	37.0	37.0	37.0	37.0
51	36.1465	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	2.0
24	0.0
25	5.0
26	5.0
27	14.0
28	17.0
29	22.0
30	47.0
31	47.0
32	68.0
33	69.0
34	113.0
35	180.0
36	1659.0
37	1751.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.02351175587794	19.084542271135568	19.459729864932466	14.432216108054027
2	20.474999999999998	8.425	18.224999999999998	52.87500000000001
3	22.825	44.4	9.6	23.175
4	26.0	11.375	41.875	20.75
5	40.699999999999996	10.274999999999999	28.000000000000004	21.025
6	58.925000000000004	9.625	13.975000000000001	17.474999999999998
7	63.1	10.2	11.875	14.825
8	20.65	10.975	54.175	14.2
9	56.55	13.900000000000002	13.100000000000001	16.45
10	63.0	10.274999999999999	11.025	15.7
11	25.474999999999998	7.575	45.725	21.224999999999998
12	22.6	12.125	48.25	17.025000000000002
13	17.325	9.025	21.75	51.9
14	20.8	52.2	14.325	12.675
15	63.575	11.725	12.325	12.375
16	22.05	14.249999999999998	52.47500000000001	11.225
17	57.325	12.35	19.925	10.4
18	62.324999999999996	13.15	14.975	9.55
19	23.325000000000003	13.750000000000002	53.225	9.700000000000001
20	18.575	49.1	23.1	9.225
21	51.949999999999996	19.325	18.325	10.4
22	22.925	47.125	20.9	9.049999999999999
23	22.275	47.525	19.575	10.625
24	19.225	16.775000000000002	51.575	12.425
25	18.8	11.875	55.025	14.299999999999999
26	20.1	10.75	22.75	46.400000000000006
27	20.525	9.025	51.775000000000006	18.675
28	24.349999999999998	8.15	20.349999999999998	47.15
29	55.525000000000006	7.199999999999999	17.675	19.6
30	23.275000000000002	8.3	17.125	51.300000000000004
31	26.025	9.625	25.124999999999996	39.225
32	37.025000000000006	10.625	35.275	17.075000000000003
33	57.599999999999994	11.799999999999999	14.825	15.775
34	50.14999999999999	25.75	11.475	12.625
35	25.5	49.45	11.425	13.625000000000002
36	23.3	40.400000000000006	21.05	15.25
37	19.6	20.225	42.625	17.549999999999997
38	16.650000000000002	21.175	34.1	28.075
39	14.774999999999999	20.9	22.75	41.575
40	17.2	19.0	31.85	31.95
41	28.299999999999997	20.025000000000002	11.225	40.45
42	50.6	19.275000000000002	9.475	20.65
43	50.7	18.7	10.325	20.275000000000002
44	40.075	18.8	21.6	19.525000000000002
45	27.900000000000002	17.575	32.5	22.025
46	36.15	14.95	13.325000000000001	35.575
47	14.099999999999998	18.15	10.775	56.974999999999994
48	13.15	30.025000000000002	10.274999999999999	46.550000000000004
49	14.2	50.3	11.125	24.375
50	26.575	39.725	10.625	23.075000000000003
51	47.3	21.55	10.975	20.175
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.5
35	1.0
36	0.5
37	0.0
38	0.5
39	1.0
40	1.0
41	1.0
42	0.5
43	0.0
44	2.5
45	5.0
46	30.5
47	56.0
48	70.5
49	85.0
50	173.0
51	261.0
52	586.5
53	912.0
54	947.0
55	982.0
56	806.0
57	630.0
58	532.0
59	434.0
60	393.5
61	353.0
62	266.0
63	179.0
64	117.0
65	55.0
66	42.5
67	30.0
68	21.0
69	12.0
70	6.5
71	1.0
72	0.5
73	0.0
74	1.0
75	1.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.65
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.19812426729192	32.925
2	13.833528722157093	11.799999999999999
3	2.9308323563892147	3.75
4	1.875732708089097	3.2
5	0.6447831184056272	1.375
6	0.6447831184056272	1.6500000000000001
7	0.3516998827667058	1.05
8	0.1758499413833529	0.6
9	0.3516998827667058	1.35
>10	1.7584994138335288	13.25
>50	0.0	0.0
>100	0.23446658851113714	29.049999999999997
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG	408	10.2	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG	350	8.75	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG	220	5.5	Illumina Small RNA Adapter 2 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG	184	4.6	Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA	43	1.075	No Hit
CGCGACCTCAGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCG	36	0.8999999999999999	RNA PCR Primer, Index 9 (97% over 37bp)
CGCGACCTCAGATCAGACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	36	0.8999999999999999	RNA PCR Primer, Index 1 (100% over 32bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG	35	0.8750000000000001	No Hit
TGGACGGCGGGGTTCTTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 32bp)
GCATTGGTTGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG	23	0.575	No Hit
GCATGGGTTGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG	22	0.5499999999999999	No Hit
AGGGCAAAAGCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT	19	0.475	RNA PCR Primer, Index 9 (100% over 36bp)
CTATGGTTACGGCTGGCGGTATGGAATTCTCGGGTGCCAAGGAACTCCAGT	16	0.4	RNA PCR Primer, Index 1 (100% over 30bp)
GACTCTTAGCGGTGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	16	0.4	RNA PCR Primer, Index 6 (100% over 34bp)
AGGGCTGGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCGCA	16	0.4	RNA PCR Primer, Index 9 (97% over 37bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAG	16	0.4	No Hit
AGGGCTGGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCGC	16	0.4	RNA PCR Primer, Index 9 (97% over 37bp)
ATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGGA	15	0.375	RNA PCR Primer, Index 1 (100% over 22bp)
TGGACGGCGGGGTTCTTGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	15	0.375	RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC	14	0.35000000000000003	RNA PCR Primer, Index 9 (100% over 37bp)
GCATGGGTTGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG	14	0.35000000000000003	Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGCTGTAGCTCAGATGGGAGAGCGCTGCAATTGGAATTCTCGGGTGCCA	13	0.325	No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGG	12	0.3	Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTTGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG	12	0.3	Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCGGATAGCTCAGCGGTAGAGCAATCGACTGGAATTCTCGGGTGCCAAG	12	0.3	No Hit
GTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC	11	0.27499999999999997	RNA PCR Primer, Index 9 (100% over 37bp)
AGGGCAAAAGCTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA	11	0.27499999999999997	RNA PCR Primer, Index 7 (97% over 35bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGG	11	0.27499999999999997	Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGGA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 22bp)
TGGACGGCGGGGTTCTTGCGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	11	0.27499999999999997	RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCAA	10	0.25	No Hit
TGGACGGCGGGGTTCTTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	10	0.25	RNA PCR Primer, Index 1 (100% over 31bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGGA	10	0.25	RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCCATAGCTCAGTTGGGAGAGCGCTTGAATTGGAATTCTCGGGTGCCA	9	0.22499999999999998	No Hit
TGGGGGATCGTCTAACGGTAGGACCCCAGACTTGGAATTCTCGGGTGCCAA	9	0.22499999999999998	No Hit
CGCGACCTCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCGC	9	0.22499999999999998	RNA PCR Primer, Index 9 (97% over 37bp)
TCATGAGAACGATCAAGTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
GACTCTTAGCGGTGGATCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 30bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 29bp)
GCGGGTGTAGCTCAGGGGTAGAGCACAACTGGAATTCTCGGGTGCCAAGGA	8	0.2	RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG	8	0.2	Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGCTGTAGCTCAGATGGGAGAGCGCTGCAATCGTGGAATTCTCGGGTGC	8	0.2	No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 28bp)
TCAGGAGAACGATCAAGTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 31bp)
GGGGCTGTAGCTCAGATGGGAGAGCGCTGCAATCTGGAATTCTCGGGTGCC	7	0.17500000000000002	No Hit
AGCGCCCGTCCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCG	7	0.17500000000000002	RNA PCR Primer, Index 9 (97% over 37bp)
ATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 23bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT	7	0.17500000000000002	RNA PCR Primer, Index 9 (100% over 36bp)
TCAGGAGAACGATCAAGTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	6	0.15	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGGA	6	0.15	RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGGAA	6	0.15	RNA PCR Primer, Index 1 (100% over 23bp)
AGCCCGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCGCAT	6	0.15	RNA PCR Primer, Index 9 (97% over 37bp)
TCATGAGAACGATCAAGTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	6	0.15	RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAGGA	6	0.15	RNA PCR Primer, Index 1 (100% over 22bp)
TTCAAAGCTAACCCAGGGAACTGAAATGGAATTCTCGGGTGCCAAGGAACT	6	0.15	RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	6	0.15	RNA PCR Primer, Index 6 (100% over 34bp)
GGGGCTGTAGCTCAGATGGGAGAGCGCTGCAATGGAATTCTCGGGTGCCAA	6	0.15	No Hit
AGGGCTGGGTCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCG	6	0.15	RNA PCR Primer, Index 9 (97% over 37bp)
GCGGGCGTAGCTCAGTTGGTTAGAGTGCCGGCCTGGAATTCTCGGGTGCCA	6	0.15	No Hit
GACTCTTAGCGGTGGATCACTCGGCTGGAATTCTCGGGTGCCAAGGAACTC	5	0.125	RNA PCR Primer, Index 1 (100% over 26bp)
CGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG	5	0.125	RNA PCR Primer, Index 6 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG	5	0.125	RNA PCR Primer, Index 1 (100% over 29bp)
GGGCTGGAGAGATGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA	5	0.125	RNA PCR Primer, Index 7 (97% over 35bp)
AGAAGCCGGCGGGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA	5	0.125	RNA PCR Primer, Index 7 (97% over 35bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTGGAATTCTCGGGTGCC	5	0.125	No Hit
GGGGCCATAGCTCAGTTGGGAGAGCGCTTGAATGGAATTCTCGGGTGCCAA	5	0.125	No Hit
AGCAGGACGGTGGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA	5	0.125	RNA PCR Primer, Index 7 (97% over 35bp)
ATGGTTACGGCTGGCGGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	5	0.125	RNA PCR Primer, Index 1 (100% over 32bp)
AGGGGGCCGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCG	5	0.125	RNA PCR Primer, Index 9 (97% over 37bp)
TGGGGGATCGTCTAACGGTAGGACCCCAGACTCTTGGAATTCTCGGGTGCC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.025	0.0	0.0	0.0
2	0.0	0.025	0.0	0.0	0.0
3	0.0	0.025	0.0	0.0	0.0
4	0.0	0.05	0.0	0.0	0.0
5	0.0	0.05	0.0	0.0	0.0
6	0.0	0.05	0.0	0.0	0.0
7	0.0	0.075	0.0	0.0	0.0
8	0.0	0.075	0.0	0.0	0.0
9	0.0	0.125	0.0	0.0	0.0
10	0.0	0.475	0.0	0.0	0.0
11	0.0	1.175	0.0	0.0	0.0
12	0.0	2.325	0.0	0.0	0.0
13	0.0	4.1	0.0	0.0	0.0
14	0.0	7.225	0.0	0.0	0.0
15	0.0	10.2	0.0	0.0	0.0
16	0.0	13.8	0.0	0.0	0.0
17	0.0	17.975	0.0	0.0	0.0
18	0.0	22.05	0.0	0.0	0.0
19	0.0	25.3	0.0	0.0	0.0
20	0.0	30.3	0.0	0.0	0.0
21	0.0	34.75	0.0	0.0	0.0
22	0.0	39.425	0.0	0.0	0.0
23	0.0	43.15	0.0	0.0	0.0
24	0.0	46.6	0.0	0.0	0.0
25	0.0	48.6	0.0	0.0	0.0
26	0.0	50.8	0.0	0.0	0.0
27	0.0	52.05	0.0	0.0	0.0
28	0.0	52.85	0.0	0.0	0.0
29	0.0	53.775	0.0	0.0	0.0
30	0.0	55.9	0.0	0.0	0.0
31	0.0	68.3	0.0	0.0	0.0
32	0.0	91.375	0.0	0.0	0.0
33	0.0	94.375	0.0	0.0	0.0
34	0.0	95.65	0.0	0.0	0.0
35	0.0	96.85	0.0	0.0	0.0
36	0.0	97.4	0.0	0.0	0.0
37	0.0	97.675	0.0	0.0	0.0
38	0.0	97.85	0.0	0.0	0.0
39	0.0	97.95	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATGGG	65	0.0	45.000004	1
CATGGGT	65	0.0	45.000004	2
ATTGGTG	25	3.236105E-5	45.0	3
GCTGGGT	20	6.222983E-4	45.0	4
AGGGCTG	20	6.222983E-4	45.0	1
GGCTGGG	20	6.222983E-4	45.0	3
TCTCGCC	70	0.0	45.0	25
GGTGGTT	85	0.0	45.0	6
TGGTGGT	35	8.569259E-8	45.0	5
ATGGGTG	60	0.0	44.999996	3
CATTGGT	30	1.6715167E-6	44.999996	2
TGGGTGG	60	0.0	44.999996	4
GCATTGG	30	1.6715167E-6	44.999996	1
GGGTGGT	60	0.0	44.999996	5
GTAGAAT	100	0.0	42.75	18
AGTGGTA	100	0.0	42.75	14
GTTCAGT	100	0.0	42.75	10
AGAATTC	100	0.0	42.75	20
TCAGTGG	100	0.0	42.75	12
TTCTCGC	100	0.0	42.75	24
>>END_MODULE
Read 4479066 spots for SRR32454947.sra
Written 4479066 spots for SRR32454947.sra
Read 4479066 spots for SRR32454947.sra
Written 4479066 spots for SRR32454947.sra
Read 4479066 spots for SRR32454947.sra
Written 4479066 spots for SRR32454947.sra
Read 4479066 spots for SRR32454947.sra
Written 4479066 spots for SRR32454947.sra
Read 4479066 spots for SRR32454947.sra
Written 4479066 spots for SRR32454947.sra
Read 4479073 spots for SRR32454947.sra
Written 4479073 spots for SRR32454947.sra
Read 4479066 spots for SRR32454947.sra
Written 4479066 spots for SRR32454947.sra
Read 4479066 spots for SRR32454947.sra
Written 4479066 spots for SRR32454947.sra
SRR ids: ['SRR32454947.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_nlsgexoh
SRR32454947.sra spots: 35832535
blocks: [[1, 4479066], [4479067, 8958132], [8958133, 13437198], [13437199, 17916264], [17916265, 22395330], [22395331, 26874396], [26874397, 31353462], [31353463, 35832535]]
SRR32454947 file size 6164260
SRR32454947 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32454947 SRR32454947_1.fastq
Input file:	SRR32454947_1.fastq
trimmed:	SRR32454947-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Sat Nov  1 19:53:57 2025 >> started

Sat Nov  1 19:54:14 2025 >> done (17.656s)
35832535 reads processed; of these:
     251 ( 0.00%) short reads filtered out after trimming by size control
       4 ( 0.00%) empty reads filtered out after trimming by size control
35832280 (100.00%) reads available; of these:
     293 ( 0.00%) trimmed reads available after processing
35831987 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 50	     293	  0.00%
 51	35831987	100.00%
35832280 reads passed initial QC


criterion=sequence-density
sequence-density=97.92
sequence-density-rank=1
fanout-score=11.20
fanout-score-rank=2
prefix-density=98.73
prefix-fanout=11.1
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCGCATCTCGTAT


criterion=fanout-score
sequence-density=2.10
sequence-density-rank=5
fanout-score=12.05
fanout-score-rank=1
prefix-density=2.09
prefix-fanout=12.1
sequence=GGCTGGAATTCT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCGCATCTCGTAT -o SRR32454947 -
Input file:	STDIN
trimmed:	SRR32454947-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCGCATCTCGTAT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Sat Nov  1 19:57:02 2025 >> started

Sat Nov  1 19:57:17 2025 >> done (15.566s)
35101009 reads processed; of these:
 7702963 (21.95%) short reads filtered out after trimming by size control
    2913 ( 0.01%) empty reads filtered out after trimming by size control
27395133 (78.05%) reads available; of these:
27322890 (99.74%) trimmed reads available after processing
   72243 ( 0.26%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	 1201211	  4.38%
 19	 1737320	  6.34%
 20	 1402077	  5.12%
 21	 1552992	  5.67%
 22	 1410020	  5.15%
 23	 1089576	  3.98%
 24	  785677	  2.87%
 25	  620168	  2.26%
 26	  454134	  1.66%
 27	  311173	  1.14%
 28	  388241	  1.42%
 29	  764349	  2.79%
 30	 4493720	 16.40%
 31	 8389467	 30.62%
 32	 1054256	  3.85%
 33	  490467	  1.79%
 34	  492236	  1.80%
 35	  291283	  1.06%
 36	  134397	  0.49%
 37	   60383	  0.22%
 38	   44164	  0.16%
 39	   48257	  0.18%
 40	   33807	  0.12%
 41	   48824	  0.18%
 42	   12490	  0.05%
 43	    3021	  0.01%
 44	    2052	  0.01%
 45	    1583	  0.01%
 46	    1432	  0.01%
 47	    2443	  0.01%
 48	    1670	  0.01%
 49	       0	  0.00%
 50	       1	  0.00%
 51	   72242	  0.26%


criterion=sequence-density
sequence-density=40.96
sequence-density-rank=1
fanout-score=2.00
fanout-score-rank=7
prefix-density=40.87
prefix-fanout=2.0
sequence=GGTGGTTCAGTGGTAGAATTCTCGCCT


criterion=fanout-score
sequence-density=1.00
sequence-density-rank=9
fanout-score=10.83
fanout-score-rank=1
prefix-density=1.81
prefix-fanout=6.0
sequence=TAGCTCAGTTGG
                                 Started job on |	Nov 01 20:00:21
                             Started mapping on |	Nov 01 20:00:21
                                    Finished on |	Nov 01 20:02:26
       Mapping speed, Million of reads per hour |	810.04

                          Number of input reads |	28126404
                      Average input read length |	23
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2689067
                        Uniquely mapped reads % |	9.56%
                          Average mapped length |	17.75
                       Number of splices: Total |	58333
            Number of splices: Annotated (sjdb) |	10189
                       Number of splices: GT/AG |	52303
                       Number of splices: GC/AG |	906
                       Number of splices: AT/AC |	6
               Number of splices: Non-canonical |	5118
                      Mismatch rate per base, % |	1.20%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.39
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.04
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	7227676
             % of reads mapped to multiple loci |	25.70%
        Number of reads mapped to too many loci |	15679649
             % of reads mapped to too many loci |	55.75%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	8.32%
                     % of reads unmapped: other |	0.67%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	18209661	18209661	18209661
N_multimapping	7227676	7227676	7227676
N_noFeature	1786411	2056679	2409739
N_ambiguous	51564	40508	2086
UnstrandedReadsAssigned:851092 PositiveStrandReadsAssigned:591880 NegativeStrandReadsAssigned:277242
Dataset is classified unstranded
MeadianReadLen=26 20thPercentileLength=17 echo kmer=19
SRR32454947 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 31,715
[index] number of k-mers: 49,362,984
[index] number of equivalence classes: 166,181
[quant] running in single-end mode
[quant] will process file 1: SRR32454947-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 28,126,404 reads, 232,186 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 635 rounds

  31716 SRR32454947.ke.tsv
  32883 SRR32454947.se.tsv
  64599 total
==> SRR32454947.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	856	0	0
ENSRNOT00000040993.4	1039	940	3	16.3277
ENSRNOT00000050156.3	1545	1446	22	77.8372
ENSRNOT00000043693.3	684	585	7	61.2174
ENSRNOT00000046201.3	204	105	2	97.4482
ENSRNOT00000046108.3	681	582	2	17.5809
ENSRNOT00000049683.3	784	685	0	0
ENSRNOT00000041241.3	348	249	0	0
ENSRNOT00000044582.3	297	198	0	0

==> SRR32454947.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	1
ENSRNOG00000058808	0
ENSRNOG00000061316	0
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	0
SRR32454947 completed mapping pipeline successfully
