Starting /dee2/code/volunteer_pipeline.sh SRR32454944
    current disk space = 1118584300
    free memory = 197967100 
SRR32454944 SRAfilesize
8748985762d3cd6d425be0422067eea9  SRR32454944.sra
SRR32454944.sra file validated
SRR32454944 is single end
SRR32454944 is conventional basespace
SRR32454944 read1 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR32454944_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.13025	37.0	37.0	37.0	37.0	37.0
2	36.2125	37.0	37.0	37.0	37.0	37.0
3	36.3155	37.0	37.0	37.0	37.0	37.0
4	36.2405	37.0	37.0	37.0	37.0	37.0
5	36.3245	37.0	37.0	37.0	37.0	37.0
6	36.3905	37.0	37.0	37.0	37.0	37.0
7	36.372	37.0	37.0	37.0	37.0	37.0
8	36.456	37.0	37.0	37.0	37.0	37.0
9	36.4135	37.0	37.0	37.0	37.0	37.0
10	36.517	37.0	37.0	37.0	37.0	37.0
11	36.4505	37.0	37.0	37.0	37.0	37.0
12	36.458	37.0	37.0	37.0	37.0	37.0
13	36.4495	37.0	37.0	37.0	37.0	37.0
14	36.523	37.0	37.0	37.0	37.0	37.0
15	36.434	37.0	37.0	37.0	37.0	37.0
16	36.374	37.0	37.0	37.0	37.0	37.0
17	36.3325	37.0	37.0	37.0	37.0	37.0
18	36.304	37.0	37.0	37.0	37.0	37.0
19	36.3945	37.0	37.0	37.0	37.0	37.0
20	36.274	37.0	37.0	37.0	37.0	37.0
21	36.2885	37.0	37.0	37.0	37.0	37.0
22	36.39	37.0	37.0	37.0	37.0	37.0
23	36.4665	37.0	37.0	37.0	37.0	37.0
24	36.3045	37.0	37.0	37.0	37.0	37.0
25	36.378	37.0	37.0	37.0	37.0	37.0
26	36.277	37.0	37.0	37.0	37.0	37.0
27	36.2665	37.0	37.0	37.0	37.0	37.0
28	36.161	37.0	37.0	37.0	37.0	37.0
29	36.2855	37.0	37.0	37.0	37.0	37.0
30	36.194	37.0	37.0	37.0	37.0	37.0
31	36.33	37.0	37.0	37.0	37.0	37.0
32	36.257	37.0	37.0	37.0	37.0	37.0
33	36.286	37.0	37.0	37.0	37.0	37.0
34	36.3205	37.0	37.0	37.0	37.0	37.0
35	36.302	37.0	37.0	37.0	37.0	37.0
36	36.037	37.0	37.0	37.0	37.0	37.0
37	36.242	37.0	37.0	37.0	37.0	37.0
38	36.3055	37.0	37.0	37.0	37.0	37.0
39	36.2645	37.0	37.0	37.0	37.0	37.0
40	36.193	37.0	37.0	37.0	37.0	37.0
41	36.119	37.0	37.0	37.0	37.0	37.0
42	36.1905	37.0	37.0	37.0	37.0	37.0
43	36.18	37.0	37.0	37.0	37.0	37.0
44	36.2655	37.0	37.0	37.0	37.0	37.0
45	36.2185	37.0	37.0	37.0	37.0	37.0
46	36.134	37.0	37.0	37.0	37.0	37.0
47	36.18	37.0	37.0	37.0	37.0	37.0
48	36.2545	37.0	37.0	37.0	37.0	37.0
49	36.0925	37.0	37.0	37.0	37.0	37.0
50	36.0485	37.0	37.0	37.0	37.0	37.0
51	35.693	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	1.0
23	0.0
24	0.0
25	5.0
26	9.0
27	18.0
28	13.0
29	28.0
30	34.0
31	49.0
32	66.0
33	83.0
34	101.0
35	208.0
36	1380.0
37	2004.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.73002754820937	20.96168294515402	16.528925619834713	16.779363886801903
2	27.675	9.175	14.825	48.325
3	25.224999999999998	40.5	9.0	25.275
4	30.625000000000004	9.9	40.1	19.375
5	38.525	11.200000000000001	27.700000000000003	22.575
6	54.825	8.7	15.65	20.825
7	58.074999999999996	12.025	12.55	17.349999999999998
8	23.25	10.725	51.449999999999996	14.575
9	55.50000000000001	13.175	11.85	19.475
10	56.65	12.925	14.95	15.475
11	28.575	6.6000000000000005	42.675000000000004	22.15
12	23.925	15.049999999999999	46.35	14.674999999999999
13	19.25	12.45	21.625	46.675
14	23.95	47.8	16.525000000000002	11.725
15	59.22500000000001	15.7	15.35	9.725
16	22.175	16.675	50.349999999999994	10.8
17	52.0	14.174999999999999	23.425	10.4
18	53.5	14.099999999999998	20.474999999999998	11.924999999999999
19	21.625	13.175	53.1	12.1
20	18.725	44.15	25.224999999999998	11.899999999999999
21	50.3	18.075	17.95	13.675
22	24.7	42.8	22.525000000000002	9.975000000000001
23	23.674999999999997	41.975	22.400000000000002	11.95
24	26.525	12.225	48.225	13.025
25	23.75	9.075	51.175000000000004	16.0
26	22.875	11.5	18.725	46.9
27	20.875	11.975	46.525	20.625
28	24.025	10.9	18.95	46.125
29	52.625	11.1	15.575	20.7
30	23.325000000000003	11.875	15.525	49.275000000000006
31	25.974999999999998	14.799999999999999	23.375	35.85
32	35.975	16.05	30.275000000000002	17.7
33	55.15	16.75	12.0	16.1
34	47.275	29.15	10.05	13.525
35	23.575	48.975	11.55	15.9
36	19.35	39.574999999999996	23.525	17.549999999999997
37	18.25	19.8	40.575	21.375
38	14.625	19.75	33.425	32.2
39	13.3	20.225	22.75	43.725
40	15.525	19.85	30.2	34.425
41	25.95	21.775	13.275	39.0
42	48.775	18.3	10.75	22.175
43	48.725	16.925	13.675	20.674999999999997
44	37.325	18.4	22.675	21.6
45	26.275	18.6	31.85	23.275000000000002
46	32.6	15.0	14.875	37.525
47	11.600000000000001	16.1	15.675	56.625
48	11.600000000000001	28.050000000000004	13.55	46.800000000000004
49	11.65	46.825	13.200000000000001	28.325
50	21.775	39.75	15.475	23.0
51	42.725	21.025	15.15	21.099999999999998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.5
29	1.0
30	1.0
31	1.0
32	1.0
33	1.0
34	0.5
35	0.0
36	0.0
37	0.0
38	0.5
39	1.0
40	0.5
41	0.0
42	1.5
43	3.0
44	8.5
45	14.0
46	41.0
47	68.0
48	87.5
49	107.0
50	167.0
51	227.0
52	569.5
53	912.0
54	931.5
55	951.0
56	856.0
57	761.0
58	595.0
59	429.0
60	349.0
61	269.0
62	206.0
63	143.0
64	106.5
65	70.0
66	46.0
67	22.0
68	18.5
69	15.0
70	9.0
71	3.0
72	2.5
73	2.0
74	1.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17500000000000002
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.16173808086904	31.55
2	13.88050694025347	11.5
3	3.8020519010259504	4.725
4	1.388050694025347	2.3
5	0.724200362100181	1.5
6	0.8449004224502112	2.1
7	0.48280024140012073	1.4000000000000001
8	0.24140012070006037	0.8
9	0.3621001810500905	1.35
>10	1.7501508750754375	12.475
>50	0.12070006035003018	2.775
>100	0.24140012070006037	27.525
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG	376	9.4	No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG	348	8.7	No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG	193	4.825	Illumina Small RNA Adapter 2 (100% over 21bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG	184	4.6	Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCAC	56	1.4000000000000001	RNA PCR Primer, Index 43 (97% over 37bp)
AGGGCTGGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCACTA	55	1.375	RNA PCR Primer, Index 43 (97% over 37bp)
CGCGACCTCAGATCAGACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	33	0.8250000000000001	RNA PCR Primer, Index 1 (100% over 32bp)
AGGGCAAAAGCTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG	28	0.7000000000000001	RNA PCR Primer, Index 2 (97% over 35bp)
CGCGACCTCAGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCA	26	0.65	RNA PCR Primer, Index 43 (97% over 37bp)
AGCGCCCGTCCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCAC	24	0.6	RNA PCR Primer, Index 43 (97% over 37bp)
CGCGACCTCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCACT	22	0.5499999999999999	RNA PCR Primer, Index 43 (97% over 37bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA	22	0.5499999999999999	RNA PCR Primer, Index 1 (100% over 22bp)
AGGGCTGGGTCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCACT	22	0.5499999999999999	RNA PCR Primer, Index 43 (97% over 37bp)
ATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGGA	20	0.5	RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAGG	20	0.5	Illumina Small RNA Adapter 2 (100% over 21bp)
AGCCCGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCACTAT	19	0.475	RNA PCR Primer, Index 43 (97% over 37bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG	18	0.44999999999999996	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA	17	0.42500000000000004	No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAG	17	0.42500000000000004	No Hit
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGC	16	0.4	RNA PCR Primer, Index 11 (97% over 36bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG	15	0.375	No Hit
TCATGAGAACGATCAAGTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	15	0.375	RNA PCR Primer, Index 1 (100% over 30bp)
GGGCTGGAGAGATGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT	15	0.375	RNA PCR Primer, Index 3 (100% over 34bp)
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG	14	0.35000000000000003	RNA PCR Primer, Index 2 (97% over 35bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGTGGAATTCTCGGGTGCCAAGGA	14	0.35000000000000003	RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCTGTAGCTCAGATGGGAGAGCGCTGCAATTGGAATTCTCGGGTGCCA	14	0.35000000000000003	No Hit
AGCGGGGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCACTAT	12	0.3	RNA PCR Primer, Index 43 (97% over 37bp)
CTATGGTTACGGCTGGCGGTATGGAATTCTCGGGTGCCAAGGAACTCCAGT	12	0.3	RNA PCR Primer, Index 1 (100% over 30bp)
TGGACGGCGGGGTTCTTGCGGATGGAATTCTCGGGTGCCAAGGAACTCCAG	12	0.3	RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAGG	11	0.27499999999999997	Illumina Small RNA Adapter 2 (100% over 21bp)
GACTCTTAGCGGTGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT	11	0.27499999999999997	RNA PCR Primer, Index 3 (100% over 34bp)
AGGGCAAAAGCTCGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGC	11	0.27499999999999997	RNA PCR Primer, Index 11 (97% over 36bp)
CGGCGCGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCACTATC	11	0.27499999999999997	RNA PCR Primer, Index 43 (97% over 37bp)
AAGCCCGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCACTA	10	0.25	RNA PCR Primer, Index 43 (97% over 37bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCAA	9	0.22499999999999998	No Hit
GGGGCTGTAGCTCAGATGGGAGAGCGCTGCATGGAATTCTCGGGTGCCAAG	9	0.22499999999999998	No Hit
TCATGAGAACGATCAAGTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	9	0.22499999999999998	RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAA	9	0.22499999999999998	No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT	9	0.22499999999999998	RNA PCR Primer, Index 3 (100% over 34bp)
GTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCA	9	0.22499999999999998	RNA PCR Primer, Index 43 (97% over 37bp)
ACTCTTAGCGGTGGATTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG	8	0.2	RNA PCR Primer, Index 2 (97% over 35bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG	8	0.2	No Hit
TGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCAC	8	0.2	RNA PCR Primer, Index 43 (97% over 37bp)
GACTCTTAGCGGTGGATCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	8	0.2	RNA PCR Primer, Index 1 (100% over 31bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 32bp)
AGCGCCCGTCCGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCA	7	0.17500000000000002	RNA PCR Primer, Index 43 (97% over 37bp)
AGCGCCCGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCACTA	7	0.17500000000000002	RNA PCR Primer, Index 43 (97% over 37bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 29bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCA	7	0.17500000000000002	RNA PCR Primer, Index 43 (97% over 37bp)
CGCGGCGCCTCGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGC	7	0.17500000000000002	RNA PCR Primer, Index 11 (97% over 36bp)
GACTCTTAGCGGTGGATCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 32bp)
GACTCTTAGCGGTGGATCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT	7	0.17500000000000002	RNA PCR Primer, Index 1 (100% over 30bp)
TCCCGGATATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCACTATC	6	0.15	RNA PCR Primer, Index 43 (97% over 37bp)
AAGGGGGGCGGTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCAC	6	0.15	RNA PCR Primer, Index 43 (97% over 37bp)
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATTGGAATTCTCGGGTGCCAAG	6	0.15	No Hit
GGGGCTGTAGCTCAGATGGGAGAGCGCTGCTGGAATTCTCGGGTGCCAAGG	6	0.15	Illumina Small RNA Adapter 2 (100% over 21bp)
GGGAGCCTGAGAAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCA	6	0.15	RNA PCR Primer, Index 43 (97% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTTGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAGG	6	0.15	Illumina Small RNA Adapter 2 (100% over 21bp)
TTCAAAGCTAACCCAGGGAACTGAATGGAATTCTCGGGTGCCAAGGAACTC	6	0.15	RNA PCR Primer, Index 1 (100% over 26bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTTGGAATTCTCGGGTGCC	6	0.15	No Hit
GACTCTTAGCGGTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG	6	0.15	RNA PCR Primer, Index 2 (97% over 35bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
TTACGGCTGGCGGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGC	6	0.15	RNA PCR Primer, Index 11 (97% over 36bp)
GACTCTTAGCGGTGGATCACTCGGTGGAATTCTCGGGTGCCAAGGAACTCC	6	0.15	RNA PCR Primer, Index 1 (100% over 27bp)
TCCCGGATAGCTCAGCGGTAGAGCAATCGACTTGGAATTCTCGGGTGCCAA	6	0.15	No Hit
CGCGGGGTGGAGCAGCCCGGTAGCTCGTCAGGCTTGGAATTCTCGGGTGCC	5	0.125	No Hit
CGCGACCTCAGATCAGACGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC	5	0.125	RNA PCR Primer, Index 1 (100% over 31bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
AGGGCTGGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCACTAT	5	0.125	RNA PCR Primer, Index 43 (97% over 37bp)
ATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGAA	5	0.125	RNA PCR Primer, Index 1 (100% over 23bp)
AGCGGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCACTATC	5	0.125	RNA PCR Primer, Index 43 (97% over 37bp)
TCATGAGAACGATCAAGTGCCTTTGGAATTCTCGGGTGCCAAGGAACTCCA	5	0.125	RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAAGGA	5	0.125	RNA PCR Primer, Index 1 (100% over 22bp)
GAAGGGCCATCGCTCAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC	5	0.125	RNA PCR Primer, Index 1 (100% over 33bp)
GGGGCTGTAGCTCAGATGGGAGAGCGCTGCAATGGAATTCTCGGGTGCCAA	5	0.125	No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAA	5	0.125	No Hit
TGGACGGCGGGGTTCTTGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT	5	0.125	RNA PCR Primer, Index 1 (100% over 30bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.15	0.0	0.0	0.0
2	0.0	0.15	0.0	0.0	0.0
3	0.0	0.15	0.0	0.0	0.0
4	0.0	0.175	0.0	0.0	0.0
5	0.0	0.175	0.0	0.0	0.0
6	0.0	0.175	0.0	0.0	0.0
7	0.0	0.325	0.0	0.0	0.0
8	0.0	0.625	0.0	0.0	0.0
9	0.0	1.575	0.0	0.0	0.0
10	0.0	3.85	0.0	0.0	0.0
11	0.0	6.55	0.0	0.0	0.0
12	0.0	10.225	0.0	0.0	0.0
13	0.0	13.55	0.0	0.0	0.0
14	0.0	18.15	0.0	0.0	0.0
15	0.0	21.775	0.0	0.0	0.0
16	0.0	25.0	0.0	0.0	0.0
17	0.0	29.15	0.0	0.0	0.0
18	0.0	32.675	0.0	0.0	0.0
19	0.0	35.275	0.0	0.0	0.0
20	0.0	39.0	0.0	0.0	0.0
21	0.0	42.025	0.0	0.0	0.0
22	0.0	45.625	0.0	0.0	0.0
23	0.0	50.15	0.0	0.0	0.0
24	0.0	53.05	0.0	0.0	0.0
25	0.0	54.475	0.0	0.0	0.0
26	0.0	55.6	0.0	0.0	0.0
27	0.0	56.375	0.0	0.0	0.0
28	0.0	56.825	0.0	0.0	0.0
29	0.0	57.55	0.0	0.0	0.0
30	0.0	59.725	0.0	0.0	0.0
31	0.0	71.3	0.0	0.0	0.0
32	0.0	92.125	0.0	0.0	0.0
33	0.0	94.75	0.0	0.0	0.0
34	0.0	95.775	0.0	0.0	0.0
35	0.0	96.725	0.0	0.0	0.0
36	0.0	97.125	0.0	0.0	0.0
37	0.0	97.425	0.0	0.0	0.0
38	0.0	97.625	0.0	0.0	0.0
39	0.0	97.75	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGTG	90	0.0	45.000004	3
CATTGGT	65	0.0	45.000004	2
ATTGGTG	65	0.0	45.000004	3
TCGCCTG	115	0.0	45.000004	27
TCTCGCC	115	0.0	45.000004	25
GCATGGG	90	0.0	45.000004	1
TTGGTGG	65	0.0	45.000004	4
TGGGTGG	90	0.0	45.000004	4
CATGGGT	90	0.0	45.000004	2
GCATTGG	65	0.0	45.000004	1
GGGTGGT	90	0.0	45.000004	5
CTCGCCT	115	0.0	45.000004	26
GTAGAAT	155	0.0	45.0	18
TGGTTCA	155	0.0	45.0	8
AGTGGTA	155	0.0	45.0	14
GGTTCAG	155	0.0	45.0	9
GTTCAGT	155	0.0	45.0	10
AGAATTC	155	0.0	45.0	20
TCAGTGG	155	0.0	45.0	12
TCTCGCT	40	4.3564796E-9	45.0	25
>>END_MODULE
Read 4480582 spots for SRR32454944.sra
Written 4480582 spots for SRR32454944.sra
Read 4480588 spots for SRR32454944.sra
Written 4480588 spots for SRR32454944.sra
Read 4480582 spots for SRR32454944.sra
Written 4480582 spots for SRR32454944.sra
Read 4480582 spots for SRR32454944.sra
Written 4480582 spots for SRR32454944.sra
Read 4480582 spots for SRR32454944.sra
Written 4480582 spots for SRR32454944.sra
Read 4480582 spots for SRR32454944.sra
Written 4480582 spots for SRR32454944.sra
Read 4480582 spots for SRR32454944.sra
Written 4480582 spots for SRR32454944.sra
Read 4480582 spots for SRR32454944.sra
Written 4480582 spots for SRR32454944.sra
SRR ids: ['SRR32454944.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd__o39xphi
SRR32454944.sra spots: 35844662
blocks: [[1, 4480582], [4480583, 8961164], [8961165, 13441746], [13441747, 17922328], [17922329, 22402910], [22402911, 26883492], [26883493, 31364074], [31364075, 35844662]]
SRR32454944 file size 6166332
SRR32454944 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR32454944 SRR32454944_1.fastq
Input file:	SRR32454944_1.fastq
trimmed:	SRR32454944-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	8
Sat Nov  1 19:38:54 2025 >> started

Sat Nov  1 19:39:36 2025 >> done (41.843s)
35844662 reads processed; of these:
    1069 ( 0.00%) short reads filtered out after trimming by size control
       8 ( 0.00%) empty reads filtered out after trimming by size control
35843585 (100.00%) reads available; of these:
     249 ( 0.00%) trimmed reads available after processing
35843336 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       1	  0.00%
 19	       0	  0.00%
 20	       0	  0.00%
 21	       0	  0.00%
 22	       0	  0.00%
 23	       0	  0.00%
 24	       0	  0.00%
 25	       0	  0.00%
 26	       0	  0.00%
 27	       0	  0.00%
 28	       0	  0.00%
 29	       0	  0.00%
 30	       0	  0.00%
 31	       0	  0.00%
 32	       0	  0.00%
 33	       0	  0.00%
 34	       0	  0.00%
 35	       0	  0.00%
 36	       0	  0.00%
 37	       0	  0.00%
 38	       0	  0.00%
 39	       0	  0.00%
 40	       0	  0.00%
 41	       0	  0.00%
 42	       0	  0.00%
 43	       0	  0.00%
 44	       0	  0.00%
 45	       0	  0.00%
 46	       0	  0.00%
 47	       0	  0.00%
 48	       0	  0.00%
 49	       0	  0.00%
 50	     248	  0.00%
 51	35843336	100.00%
35843585 reads passed initial QC


criterion=sequence-density
sequence-density=97.98
sequence-density-rank=1
fanout-score=12.43
fanout-score-rank=2
prefix-density=98.56
prefix-fanout=12.4
sequence=TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCACTATCTCGTATGCC


criterion=fanout-score
sequence-density=2.65
sequence-density-rank=3
fanout-score=17.79
fanout-score-rank=1
prefix-density=2.64
prefix-fanout=17.8
sequence=GGCTGGAATTCT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -t 8 -x TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCACTATCTCGTATGCC -o SRR32454944 -
Input file:	STDIN
trimmed:	SRR32454944-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGCACTATCTCGTATGCC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	8
Sat Nov  1 19:41:48 2025 >> started

Sat Nov  1 19:42:13 2025 >> done (25.062s)
35112083 reads processed; of these:
11357351 (32.35%) short reads filtered out after trimming by size control
   16927 ( 0.05%) empty reads filtered out after trimming by size control
23737805 (67.61%) reads available; of these:
23658945 (99.67%) trimmed reads available after processing
   78860 ( 0.33%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  945686	  3.98%
 19	 1370717	  5.77%
 20	 1103299	  4.65%
 21	 1357823	  5.72%
 22	 1369369	  5.77%
 23	  967911	  4.08%
 24	  566505	  2.39%
 25	  412256	  1.74%
 26	  286530	  1.21%
 27	  205041	  0.86%
 28	  302018	  1.27%
 29	  684033	  2.88%
 30	 4241585	 17.87%
 31	 7627499	 32.13%
 32	 1004572	  4.23%
 33	  395008	  1.66%
 34	  340972	  1.44%
 35	  195060	  0.82%
 36	   81760	  0.34%
 37	   44786	  0.19%
 38	   31410	  0.13%
 39	   30482	  0.13%
 40	   26587	  0.11%
 41	   39332	  0.17%
 42	   15724	  0.07%
 43	    4000	  0.02%
 44	    2810	  0.01%
 45	    1780	  0.01%
 46	    1225	  0.01%
 47	    1905	  0.01%
 48	    1261	  0.01%
 49	       0	  0.00%
 50	       5	  0.00%
 51	   78854	  0.33%


criterion=sequence-density
sequence-density=44.25
sequence-density-rank=1
fanout-score=2.00
fanout-score-rank=6
prefix-density=44.15
prefix-fanout=2.0
sequence=GGTGGTTCAGTGGTAGAATTCTCGCCTG


criterion=fanout-score
sequence-density=0.27
sequence-density-rank=28
fanout-score=9.95
fanout-score-rank=1
prefix-density=1.79
prefix-fanout=1.5
sequence=GGGAGAGCGCTTGAATG
                                 Started job on |	Nov 01 19:44:11
                             Started mapping on |	Nov 01 19:44:12
                                    Finished on |	Nov 01 19:45:28
       Mapping speed, Million of reads per hour |	1159.07

                          Number of input reads |	24469307
                      Average input read length |	24
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2351795
                        Uniquely mapped reads % |	9.61%
                          Average mapped length |	18.01
                       Number of splices: Total |	43465
            Number of splices: Annotated (sjdb) |	9150
                       Number of splices: GT/AG |	37432
                       Number of splices: GC/AG |	549
                       Number of splices: AT/AC |	2
               Number of splices: Non-canonical |	5482
                      Mismatch rate per base, % |	0.93%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.20
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.04
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	5771793
             % of reads mapped to multiple loci |	23.59%
        Number of reads mapped to too many loci |	14323581
             % of reads mapped to too many loci |	58.54%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	7.64%
                     % of reads unmapped: other |	0.62%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	16345719	16345719	16345719
N_multimapping	5771793	5771793	5771793
N_noFeature	1303461	1498377	2148209
N_ambiguous	73361	63471	1266
UnstrandedReadsAssigned:974973 PositiveStrandReadsAssigned:789947 NegativeStrandReadsAssigned:202320
Dataset is classified unstranded
MeadianReadLen=26 20thPercentileLength=17 echo kmer=19
SRR32454944 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 31,715
[index] number of k-mers: 49,362,984
[index] number of equivalence classes: 166,181
[quant] running in single-end mode
[quant] will process file 1: SRR32454944-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 24,469,307 reads, 208,067 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 703 rounds

  31716 SRR32454944.ke.tsv
  32883 SRR32454944.se.tsv
  64599 total
==> SRR32454944.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
ENSRNOT00000047550.4	955	856	1	5.07616
ENSRNOT00000040993.4	1039	940	2	9.24509
ENSRNOT00000050156.3	1545	1446	33	99.1642
ENSRNOT00000043693.3	684	585	6	44.5661
ENSRNOT00000046201.3	204	105	0	0
ENSRNOT00000046108.3	681	582	3	22.3979
ENSRNOT00000049683.3	784	685	3	19.03
ENSRNOT00000041241.3	348	249	0	0
ENSRNOT00000044582.3	297	198	0	0

==> SRR32454944.se.tsv <==
ENSRNOG00000046319	0
ENSRNOG00000047964	0
ENSRNOG00000050370	0
ENSRNOG00000032365	0
ENSRNOG00000040300	0
ENSRNOG00000058808	0
ENSRNOG00000061316	0
ENSRNOG00000050129	0
ENSRNOG00000040316	0
ENSRNOG00000023659	0
SRR32454944 completed mapping pipeline successfully
