Starting /dee2/code/volunteer_pipeline.sh SRR27822265
    current disk space = 218223712
    free memory = 128599332 
SRR27822265 SRAfilesize
76acfe94507f9d71a06dce54951c50a0  SRR27822265.sra
SRR27822265.sra file validated
SRR27822265 is paired end
SRR27822265 is conventional basespace
SRR27822265 read1 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR27822265_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.38975	37.0	37.0	37.0	37.0	37.0
2	36.5095	37.0	37.0	37.0	37.0	37.0
3	36.7185	37.0	37.0	37.0	37.0	37.0
4	36.686	37.0	37.0	37.0	37.0	37.0
5	36.613	37.0	37.0	37.0	37.0	37.0
6	36.6775	37.0	37.0	37.0	37.0	37.0
7	36.5695	37.0	37.0	37.0	37.0	37.0
8	36.603	37.0	37.0	37.0	37.0	37.0
9	36.639	37.0	37.0	37.0	37.0	37.0
10	36.67	37.0	37.0	37.0	37.0	37.0
11	36.6415	37.0	37.0	37.0	37.0	37.0
12	36.6465	37.0	37.0	37.0	37.0	37.0
13	36.6925	37.0	37.0	37.0	37.0	37.0
14	36.66	37.0	37.0	37.0	37.0	37.0
15	36.627	37.0	37.0	37.0	37.0	37.0
16	36.631	37.0	37.0	37.0	37.0	37.0
17	36.5805	37.0	37.0	37.0	37.0	37.0
18	36.6215	37.0	37.0	37.0	37.0	37.0
19	36.537	37.0	37.0	37.0	37.0	37.0
20	36.5845	37.0	37.0	37.0	37.0	37.0
21	36.68	37.0	37.0	37.0	37.0	37.0
22	36.653	37.0	37.0	37.0	37.0	37.0
23	36.5935	37.0	37.0	37.0	37.0	37.0
24	36.5475	37.0	37.0	37.0	37.0	37.0
25	36.5135	37.0	37.0	37.0	37.0	37.0
26	36.512	37.0	37.0	37.0	37.0	37.0
27	36.5975	37.0	37.0	37.0	37.0	37.0
28	36.53	37.0	37.0	37.0	37.0	37.0
29	36.5455	37.0	37.0	37.0	37.0	37.0
30	36.5735	37.0	37.0	37.0	37.0	37.0
31	36.508	37.0	37.0	37.0	37.0	37.0
32	36.522	37.0	37.0	37.0	37.0	37.0
33	36.4815	37.0	37.0	37.0	37.0	37.0
34	36.5575	37.0	37.0	37.0	37.0	37.0
35	36.6005	37.0	37.0	37.0	37.0	37.0
36	36.4565	37.0	37.0	37.0	37.0	37.0
37	36.5255	37.0	37.0	37.0	37.0	37.0
38	36.484	37.0	37.0	37.0	37.0	37.0
39	36.509	37.0	37.0	37.0	37.0	37.0
40	36.3705	37.0	37.0	37.0	37.0	37.0
41	36.525	37.0	37.0	37.0	37.0	37.0
42	36.4455	37.0	37.0	37.0	37.0	37.0
43	36.455	37.0	37.0	37.0	37.0	37.0
44	36.516	37.0	37.0	37.0	37.0	37.0
45	36.478	37.0	37.0	37.0	37.0	37.0
46	36.552	37.0	37.0	37.0	37.0	37.0
47	36.5115	37.0	37.0	37.0	37.0	37.0
48	36.484	37.0	37.0	37.0	37.0	37.0
49	36.444	37.0	37.0	37.0	37.0	37.0
50	36.354	37.0	37.0	37.0	37.0	37.0
51	36.3395	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
26	1.0
27	2.0
28	12.0
29	10.0
30	20.0
31	24.0
32	17.0
33	35.0
34	57.0
35	151.0
36	1877.0
37	1794.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.545706371191137	10.878871820700075	8.587257617728532	56.98816419038025
2	19.85	12.8	36.15	31.2
3	19.825	16.75	22.75	40.675
4	22.325	24.675	22.275	30.725
5	22.125	31.3	26.55	20.025000000000002
6	17.9	34.275	27.1	20.724999999999998
7	14.85	23.025000000000002	42.3	19.825
8	16.475	22.825	35.6	25.1
9	14.475	23.674999999999997	36.3	25.55
10	17.8	36.975	24.75	20.474999999999998
11	21.675	28.449999999999996	21.3	28.575
12	21.275	23.9	27.150000000000002	27.675
13	19.900000000000002	23.825	29.875	26.400000000000002
14	18.75	25.5	29.75	26.0
15	18.0	25.124999999999996	30.825000000000003	26.05
16	19.7	25.525	29.45	25.324999999999996
17	16.950000000000003	26.950000000000003	29.349999999999998	26.75
18	19.1	25.15	30.15	25.6
19	20.3	25.924999999999997	28.4	25.374999999999996
20	19.650000000000002	27.975	29.65	22.725
21	20.8	28.499999999999996	27.450000000000003	23.25
22	18.9	27.500000000000004	29.275000000000002	24.325
23	18.95	27.075	28.199999999999996	25.775
24	17.95	29.5	27.275	25.275
25	18.625	27.250000000000004	28.025	26.1
26	18.025	29.349999999999998	28.1	24.525
27	18.0	28.7	26.424999999999997	26.875
28	20.825	26.55	27.150000000000002	25.474999999999998
29	18.099999999999998	27.275	30.8	23.825
30	19.0	27.675	28.95	24.375
31	17.4	28.325	29.975	24.3
32	21.0	27.675	27.35	23.974999999999998
33	19.975	27.175	29.825000000000003	23.025000000000002
34	18.075	27.700000000000003	27.800000000000004	26.424999999999997
35	20.849999999999998	26.474999999999998	28.175	24.5
36	18.75	25.874999999999996	26.224999999999998	29.15
37	18.15	29.925	25.224999999999998	26.700000000000003
38	20.125	27.925	26.525	25.424999999999997
39	19.650000000000002	27.55	26.55	26.25
40	16.975	26.625	30.45	25.95
41	21.0	25.900000000000002	27.55	25.55
42	22.625	28.449999999999996	25.874999999999996	23.05
43	20.599999999999998	25.374999999999996	29.425	24.6
44	21.9	25.974999999999998	27.05	25.074999999999996
45	21.55	27.650000000000002	25.924999999999997	24.875
46	22.5	25.5	25.5	26.5
47	22.125	28.225	27.474999999999998	22.175
48	21.125	27.474999999999998	26.025	25.374999999999996
49	19.950000000000003	27.675	28.499999999999996	23.875
50	18.525	28.275	28.375	24.825
51	19.6	27.825	29.25	23.325000000000003
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.0
14	0.0
15	1.5
16	3.0
17	2.5
18	2.0
19	2.5
20	3.0
21	5.5
22	8.0
23	11.5
24	15.0
25	13.5
26	15.5
27	19.0
28	35.5
29	52.0
30	69.0
31	86.0
32	112.5
33	139.0
34	150.0
35	161.0
36	190.0
37	219.0
38	268.0
39	317.0
40	317.0
41	317.0
42	332.0
43	347.0
44	355.5
45	364.0
46	359.5
47	355.0
48	369.5
49	384.0
50	351.0
51	318.0
52	294.5
53	271.0
54	236.5
55	202.0
56	199.0
57	196.0
58	151.0
59	106.0
60	75.0
61	44.0
62	37.5
63	31.0
64	20.0
65	9.0
66	10.0
67	11.0
68	9.5
69	8.0
70	4.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.7250000000000001
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.2217659137577	46.400000000000006
2	11.868583162217659	14.45
3	4.59958932238193	8.4
4	2.299794661190965	5.6000000000000005
5	1.273100616016427	3.875
6	1.0266940451745379	3.75
7	0.6570841889117043	2.8000000000000003
8	0.6570841889117043	3.2
9	0.20533880903490762	1.125
>10	1.190965092402464	10.4
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCA	23	0.575	No Hit
ACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAAATTCTCCGCTCTG	22	0.5499999999999999	No Hit
CTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCT	20	0.5	No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCA	19	0.475	No Hit
CCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAAT	19	0.475	No Hit
CGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAAATTCTCCGCTCTGA	18	0.44999999999999996	No Hit
CCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGT	16	0.4	No Hit
CGCTTGACTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGGCACCTC	16	0.4	No Hit
CGGTAACATTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAA	16	0.4	No Hit
CTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATAAC	15	0.375	No Hit
CCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAA	15	0.375	No Hit
CTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAGTTT	14	0.35000000000000003	No Hit
CCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGGAAC	14	0.35000000000000003	No Hit
CCCAAAGTTGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATT	14	0.35000000000000003	No Hit
GTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTA	14	0.35000000000000003	No Hit
CTCGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAACG	13	0.325	No Hit
CACCAACTAAGAACGGCCATGCACCACCACCCACAAAATCAAGAAAGAGCT	13	0.325	No Hit
CACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGCTT	13	0.325	No Hit
CCTGTGGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAAAG	12	0.3	No Hit
GTAACATTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACC	12	0.3	No Hit
CCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTT	12	0.3	No Hit
GTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACA	12	0.3	No Hit
CCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAA	12	0.3	No Hit
CCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAAA	11	0.27499999999999997	No Hit
CTAGAATTACCACAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGA	11	0.27499999999999997	No Hit
CAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATT	10	0.25	No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	10	0.25	No Hit
GTACTTTTTCACTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTAC	10	0.25	No Hit
GCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAA	10	0.25	No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCA	9	0.22499999999999998	No Hit
GCATGTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAACT	9	0.22499999999999998	No Hit
CTAGAAACCAACAAAATAGAACCAAACGTCCTATTCTATTATTCCATGCTA	9	0.22499999999999998	No Hit
CTCCAATTGTTCCTCGTTAAGGTATTTACATTGTACTCATTCCAATTACAA	9	0.22499999999999998	No Hit
CCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAGCCT	9	0.22499999999999998	No Hit
CCAGATTTCAAATTTGAGCTTTTGCCGCTTCACTCGCCGTTACTAAGGCAA	8	0.2	No Hit
CTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTCGCAG	8	0.2	No Hit
CCCAGCACGACGGAGTTTCACAAGATTACCAAGACCTCTCGGCCAAGGTTA	8	0.2	No Hit
CCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTGA	8	0.2	No Hit
CTCTTACTCAAATCCATCCGAAGACATCAGGATCGGTCGATTGTGCACCTC	8	0.2	No Hit
CCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTATTCTAT	8	0.2	No Hit
CCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAGTT	8	0.2	No Hit
TGACAATTGAATACTGATGCCCCCGACCGTCCCTATTAATCATTACGATGG	8	0.2	No Hit
CTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATAC	8	0.2	No Hit
CTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAA	8	0.2	No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGG	8	0.2	No Hit
CCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTA	8	0.2	No Hit
GTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAG	8	0.2	No Hit
CCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAATG	8	0.2	No Hit
ATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGC	8	0.2	No Hit
GGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAAAGGATCG	8	0.2	No Hit
CCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCCGA	7	0.17500000000000002	No Hit
ATTCGCTTTACCTCATAAAACTGATACGAGCTTCTGCTATCCTGAGGGAAA	7	0.17500000000000002	No Hit
CTTGGCAAATGCTTTCGCAGTAGTTAGTCTTCAATAAATCCAAGAATTTCA	7	0.17500000000000002	No Hit
CTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCCTTAGGACATC	7	0.17500000000000002	No Hit
GTCCGGCTGTCTAGATGAACTAACACCTTTTGTGGTGTCTGATGAGCGTGT	7	0.17500000000000002	No Hit
CAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACC	7	0.17500000000000002	No Hit
CTGTGGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAAAGG	7	0.17500000000000002	No Hit
CCTCGTTAAGGTATTTACATTGTACTCATTCCAATTACAAGACCCGAATGG	7	0.17500000000000002	No Hit
CCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCCGAA	7	0.17500000000000002	No Hit
TACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAAATTCTCCGCTCT	7	0.17500000000000002	No Hit
CCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAAGAAT	7	0.17500000000000002	No Hit
CTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACTAG	7	0.17500000000000002	No Hit
CTTCATTCAAATGTCCACGTTCAATTAAGTAACAAGGACTTCTTACATATT	7	0.17500000000000002	No Hit
CTCCGCTTCATTGAATAAGTAAAGAAACTATAAAGGTAGTGGTATTTCACT	7	0.17500000000000002	No Hit
CCTGAATTAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGG	7	0.17500000000000002	No Hit
CAACAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCA	7	0.17500000000000002	No Hit
CCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTCGCA	6	0.15	No Hit
CTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTATCG	6	0.15	No Hit
CTCCACGCCTGCCTACTCGTCAGGGCATCATATCAACCCTGACGGTAGAGT	6	0.15	No Hit
GTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAACGGCC	6	0.15	No Hit
CCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAAT	6	0.15	No Hit
GACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTT	6	0.15	No Hit
CTCATTTGAATATTTGCTACTACCACCAAGATCTGCACTAGAGGCCGTTCG	6	0.15	No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAA	6	0.15	No Hit
CCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAATAG	6	0.15	No Hit
CTGCCACCAAAACTGATGCTGGCCCAGTGAAATGCGAGATTCCCCTACCCA	6	0.15	No Hit
GGCATATAACCATTATGCCAGCATCCTTGACTTACGTCGCAGTCCTCAGTC	6	0.15	No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTT	6	0.15	No Hit
CCGCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTGACATTCAGAGCA	6	0.15	No Hit
CTGTTATTGCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGA	6	0.15	No Hit
GTCTGATCAAATGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCT	6	0.15	No Hit
ATTTATTGTCACTACCTCCCTGAATTAGGATTGGGTAATTTGCGCGCCTGC	6	0.15	No Hit
CCACTATTTAGTAGGTTAAGGTCTCGTTCGTTATCGCAATTAAGCAGACAA	6	0.15	No Hit
CTCTAAGAAGTGGATAACCAGCAAATGCTAGCACCACTATTTAGTAGGTTA	6	0.15	No Hit
CCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGGCACCTCTAGCCTCAAA	6	0.15	No Hit
CTGATCAAATGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCT	6	0.15	No Hit
AAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCC	6	0.15	No Hit
CCCGGAACCTCTAATCATTCGCTTTACCTCATAAAACTGATACGAGCTTCT	6	0.15	No Hit
CCCCAAAGTTGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGAT	6	0.15	No Hit
CACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTT	6	0.15	No Hit
CCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGGAACC	6	0.15	No Hit
AGCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCC	5	0.125	No Hit
CCTGCCACCAAAACTGATGCTGGCCCAGTGAAATGCGAGATTCCCCTACCC	5	0.125	No Hit
GTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTTATACCATG	5	0.125	No Hit
GTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTA	5	0.125	No Hit
CCAACAGCTATGCTCTTACTCAAATCCATCCGAAGACATCAGGATCGGTCG	5	0.125	No Hit
CCCACTAAAAGCTCTTCATTCAAATGTCCACGTTCAATTAAGTAACAAGGA	5	0.125	No Hit
CCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAGC	5	0.125	No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTG	5	0.125	No Hit
TCTTCATTCAAATGTCCACGTTCAATTAAGTAACAAGGACTTCTTACATAT	5	0.125	No Hit
GCCACACTTTCATGGTTTGTATTCACACTGAAAATCAAAATCAAGGGGGCT	5	0.125	No Hit
CTCGTAAGGTGCCGAGTGGGTCATTAAAAAAACACCACCCGATCCCTAGTC	5	0.125	No Hit
AGCATGTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAAC	5	0.125	No Hit
GTCTGACCAAGGCCCTCACTACCCGACCCTTAGAGCCAATCCTTATCCCGA	5	0.125	No Hit
GCTACTACCACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGT	5	0.125	No Hit
CATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGAT	5	0.125	No Hit
CTCACTACCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTAT	5	0.125	No Hit
CGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCA	5	0.125	No Hit
CGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTTCGCCA	5	0.125	No Hit
CCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAATGAC	5	0.125	No Hit
CCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTTCGCCAAG	5	0.125	No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCG	5	0.125	No Hit
CTTTGAAACGGAGCTTCCCCATCTCTTAGGATCGACTAACCCACGTCCAAC	5	0.125	No Hit
GTCTCGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAA	5	0.125	No Hit
CCCTAGTCGGCATAGTTTATGGTTAAGACTACGACGGTATCTGATCATCTT	5	0.125	No Hit
CAGATTTCAAATTTGAGCTTTTGCCGCTTCACTCGCCGTTACTAAGGCAAT	5	0.125	No Hit
CTCCACATTCAGTTACGTTACCGTGAAGAATCCATATCCAGGTTCCGGAAT	5	0.125	No Hit
ATAAAACTGATACGAGCTTCTGCTATCCTGAGGGAAACTTCGGCAGGAACC	5	0.125	No Hit
CTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTT	5	0.125	No Hit
ACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTA	5	0.125	No Hit
CGCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGA	5	0.125	No Hit
GCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCCG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR27822265 read2 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR27822265_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.4525	37.0	37.0	37.0	37.0	37.0
2	36.39	37.0	37.0	37.0	37.0	37.0
3	36.351	37.0	37.0	37.0	37.0	37.0
4	36.3685	37.0	37.0	37.0	37.0	37.0
5	36.3895	37.0	37.0	37.0	37.0	37.0
6	36.349	37.0	37.0	37.0	37.0	37.0
7	36.3635	37.0	37.0	37.0	37.0	37.0
8	36.4565	37.0	37.0	37.0	37.0	37.0
9	36.435	37.0	37.0	37.0	37.0	37.0
10	36.4445	37.0	37.0	37.0	37.0	37.0
11	36.354	37.0	37.0	37.0	37.0	37.0
12	36.4245	37.0	37.0	37.0	37.0	37.0
13	36.38625	37.0	37.0	37.0	37.0	37.0
14	36.479	37.0	37.0	37.0	37.0	37.0
15	36.3715	37.0	37.0	37.0	37.0	37.0
16	36.34	37.0	37.0	37.0	37.0	37.0
17	36.377	37.0	37.0	37.0	37.0	37.0
18	36.442	37.0	37.0	37.0	37.0	37.0
19	36.3585	37.0	37.0	37.0	37.0	37.0
20	36.33	37.0	37.0	37.0	37.0	37.0
21	36.32	37.0	37.0	37.0	37.0	37.0
22	36.3375	37.0	37.0	37.0	37.0	37.0
23	36.355	37.0	37.0	37.0	37.0	37.0
24	36.3555	37.0	37.0	37.0	37.0	37.0
25	36.4145	37.0	37.0	37.0	37.0	37.0
26	36.498	37.0	37.0	37.0	37.0	37.0
27	36.306	37.0	37.0	37.0	37.0	37.0
28	36.4305	37.0	37.0	37.0	37.0	37.0
29	36.2875	37.0	37.0	37.0	37.0	37.0
30	36.30425	37.0	37.0	37.0	37.0	37.0
31	36.24575	37.0	37.0	37.0	37.0	37.0
32	36.3365	37.0	37.0	37.0	37.0	37.0
33	36.2635	37.0	37.0	37.0	37.0	37.0
34	36.212	37.0	37.0	37.0	37.0	37.0
35	36.2245	37.0	37.0	37.0	37.0	37.0
36	36.25775	37.0	37.0	37.0	37.0	37.0
37	36.0945	37.0	37.0	37.0	37.0	37.0
38	36.194	37.0	37.0	37.0	37.0	37.0
39	36.176	37.0	37.0	37.0	37.0	37.0
40	36.226	37.0	37.0	37.0	37.0	37.0
41	36.3455	37.0	37.0	37.0	37.0	37.0
42	36.08175	37.0	37.0	37.0	37.0	37.0
43	36.1615	37.0	37.0	37.0	37.0	37.0
44	36.2365	37.0	37.0	37.0	37.0	37.0
45	36.204	37.0	37.0	37.0	37.0	37.0
46	36.198	37.0	37.0	37.0	37.0	37.0
47	36.152	37.0	37.0	37.0	37.0	37.0
48	36.0865	37.0	37.0	37.0	37.0	37.0
49	36.1915	37.0	37.0	37.0	37.0	37.0
50	36.07525	37.0	37.0	37.0	37.0	37.0
51	36.1425	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	3.0
19	2.0
20	2.0
21	1.0
22	2.0
23	4.0
24	1.0
25	2.0
26	2.0
27	2.0
28	5.0
29	5.0
30	13.0
31	22.0
32	23.0
33	35.0
34	112.0
35	405.0
36	2148.0
37	1208.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	29.439719859929962	19.459729864932466	13.056528264132067	38.0440220110055
2	27.725	22.25	35.6	14.424999999999999
3	19.375	26.924999999999997	31.474999999999998	22.225
4	23.150000000000002	35.525	23.875	17.45
5	25.86293146573287	34.04202101050525	22.861430715357677	17.2336168084042
6	22.775000000000002	35.699999999999996	22.425	19.1
7	21.05	20.549999999999997	38.95	19.45
8	23.411705852926463	23.28664332166083	29.689844922461226	23.611805902951478
9	25.0	23.225	34.0	17.775
10	19.909954977488745	37.44372186093047	25.237618809404704	17.408704352176088
11	28.849999999999998	25.95	21.475	23.724999999999998
12	26.43821910955478	24.16208104052026	26.488244122061033	22.911455727863935
13	23.755938984746187	27.731932983245812	26.056514128532132	22.455613903475868
14	24.425	27.925	28.125	19.525000000000002
15	23.686843421710854	30.76538269134567	26.713356678339167	18.8344172086043
16	26.025	27.075	27.3	19.6
17	26.974999999999998	29.725	24.3	19.0
18	26.463231615807903	28.23911955977989	24.68734367183592	20.610305152576288
19	24.349999999999998	27.3	26.85	21.5
20	23.21160580290145	29.864932466233117	28.61430715357679	18.309154577288645
21	24.6	29.349999999999998	26.974999999999998	19.075
22	25.95	28.000000000000004	27.85	18.2
23	25.025	29.625	26.450000000000003	18.9
24	24.95	30.65	25.4	19.0
25	24.325	27.950000000000003	27.474999999999998	20.25
26	26.125	28.875	26.05	18.95
27	25.374999999999996	29.65	26.724999999999998	18.25
28	24.975	28.9	26.5	19.625
29	27.125	29.549999999999997	25.074999999999996	18.25
30	24.55613903475869	29.307326831707925	26.581645411352838	19.554888722180543
31	26.406601650412604	28.632158039509875	26.206551637909474	18.754688672168044
32	25.0	28.499999999999996	26.775	19.725
33	25.237618809404704	30.365182591295646	26.063031515757878	18.33416708354177
34	23.961980990495245	29.764882441220607	26.038019009504755	20.23511755877939
35	23.425	31.85	26.85	17.875
36	25.906476619154787	27.831957989497376	27.731932983245812	18.529632408102024
37	24.2	28.7	27.200000000000003	19.900000000000002
38	25.987993996998497	27.66383191595798	27.5887943971986	18.75937968984492
39	25.724999999999998	29.825000000000003	25.324999999999996	19.125
40	23.21160580290145	29.789894947473737	26.88844422211106	20.110055027513756
41	26.538269134567283	28.46423211605803	25.737868934467233	19.259629814907452
42	23.280820205051263	29.03225806451613	26.481620405101275	21.205301325331334
43	24.175	29.65	26.35	19.825
44	24.312156078039017	28.58929464732366	26.138069034517258	20.96048024012006
45	24.337168584292147	29.63981990995498	26.76338169084542	19.259629814907452
46	25.287643821910955	30.09004502251126	25.76288144072036	18.859429714857427
47	24.65	29.5	27.125	18.725
48	25.18759379689845	30.240120060030012	25.737868934467233	18.8344172086043
49	22.611305652826413	31.49074537268634	27.613806903451728	18.284142071035518
50	23.95598899724931	30.15753938484621	26.60665166291573	19.279819954988746
51	25.387693846923458	28.639319659829916	27.988994497248626	17.983991995998
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	1.0
17	1.5
18	2.0
19	5.0
20	8.0
21	6.5
22	5.0
23	9.5
24	14.0
25	20.0
26	30.5
27	35.0
28	47.0
29	59.0
30	94.0
31	129.0
32	133.5
33	138.0
34	162.0
35	186.0
36	208.5
37	231.0
38	236.5
39	242.0
40	310.5
41	379.0
42	416.0
43	453.0
44	441.5
45	430.0
46	413.0
47	396.0
48	356.5
49	317.0
50	306.0
51	295.0
52	254.0
53	213.0
54	177.5
55	142.0
56	134.0
57	126.0
58	96.5
59	67.0
60	49.5
61	32.0
62	32.0
63	32.0
64	29.0
65	26.0
66	17.5
67	9.0
68	4.5
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	1.0
76	2.0
77	1.5
78	1.0
79	1.0
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	1.0
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05
2	0.0
3	0.0
4	0.0
5	0.05
6	0.0
7	0.0
8	0.05
9	0.0
10	0.05
11	0.0
12	0.05
13	0.025
14	0.0
15	0.05
16	0.0
17	0.0
18	0.05
19	0.0
20	0.05
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.025
31	0.025
32	0.0
33	0.05
34	0.05
35	0.0
36	0.025
37	0.0
38	0.05
39	0.0
40	0.05
41	0.05
42	0.025
43	0.0
44	0.05
45	0.05
46	0.05
47	0.0
48	0.05
49	0.05
50	0.025
51	0.05
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.44796035074343	49.475
2	14.487228364468166	19.0
3	5.108654212733511	10.05
4	2.134959969500572	5.6000000000000005
5	0.9149828440716736	3.0
6	0.41936713686618377	1.6500000000000001
7	0.30499428135722456	1.4000000000000001
8	0.19062142584826536	1.0
9	0.30499428135722456	1.7999999999999998
>10	0.6862371330537552	7.025
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTG	30	0.75	No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG	25	0.625	No Hit
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGA	23	0.575	No Hit
CCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAA	22	0.5499999999999999	No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGT	20	0.5	No Hit
CGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATT	18	0.44999999999999996	No Hit
GGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAAAAGCCCCCTTGATTTT	15	0.375	No Hit
GGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATG	15	0.375	No Hit
CTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAGT	13	0.325	No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT	12	0.3	No Hit
GTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGA	12	0.3	No Hit
TCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG	12	0.3	No Hit
GTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTTAACCTACTAAAT	11	0.27499999999999997	No Hit
AGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACC	11	0.27499999999999997	No Hit
GGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAA	11	0.27499999999999997	No Hit
CTTAGACGTTCTGGGCCGCACGCGCGCTACACTGACGGAGCCAGCGAGTCT	11	0.27499999999999997	No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGT	10	0.25	No Hit
CACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGAT	10	0.25	No Hit
CTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAAA	9	0.22499999999999998	No Hit
ACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATT	9	0.22499999999999998	No Hit
CTTAACCATAAACTATGCCGACTAGGGATCGGGTGGTGTTTTTTTAATGAC	9	0.22499999999999998	No Hit
CGAACGTAGAGTTAAGGTGCCGGAATACACGCTCATCAGACACCACAAAAG	9	0.22499999999999998	No Hit
CTTGGATTTATTGAAGACTAACTACTGCGAAAGCATTTGCCAAGGACGTTT	9	0.22499999999999998	No Hit
ACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTT	9	0.22499999999999998	No Hit
CAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA	9	0.22499999999999998	No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACG	9	0.22499999999999998	No Hit
GTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTA	8	0.2	No Hit
CAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCTA	8	0.2	No Hit
CTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAGAAGG	8	0.2	No Hit
ATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTATGCCT	8	0.2	No Hit
GTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAA	8	0.2	No Hit
CGCAGATGTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACA	7	0.17500000000000002	No Hit
GTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAA	7	0.17500000000000002	No Hit
CGGCTCTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCA	7	0.17500000000000002	No Hit
GGCTTAGTGAGGCCTCAGGATCTGCTTAGAGAAGGGGGCAACTCCATCTCA	7	0.17500000000000002	No Hit
CCCAATCCTAATTCAGGGAGGTAGTGACAATAAATAACGATACAGGGCCCA	7	0.17500000000000002	No Hit
CGGAATTTGAGGCTAGAGGTGCCAGAAAAGTTACCACAGGGATAACTGGCT	7	0.17500000000000002	No Hit
ATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAG	7	0.17500000000000002	No Hit
CTCAAACTTTAAATATGTAAGAAGTCCTTGTTACTTAATTGAACGTGGACA	7	0.17500000000000002	No Hit
CTTAATTGAACGTGGACATTTGAATGAAGAGCTTTTAGTGGGCCATTTTTG	6	0.15	No Hit
GTAAAACCCATACGCGTAATGAAAGTGAACGTAGGTTGGGGCCTCGCAAGA	6	0.15	No Hit
CCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTTATCCACTTCTTA	6	0.15	No Hit
GGGGAATCTCGCATTTCACTGGGCCAGCATCAGTTTTGGTGGCAGGATAAA	6	0.15	No Hit
CTCGAATATATTAGCATGGAATAATAGAATAGGACGTTTGGTTCTATTTTG	6	0.15	No Hit
AACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCT	6	0.15	No Hit
GTTCCACGTCAACAGCAGTTGGACGTGGGTTAGTCGATCCTAAGAGATGGG	6	0.15	No Hit
GTCGAAATGACCTTGACCTATTCTCAAACTTTAAATATGTAAGAAGTCCTT	6	0.15	No Hit
CCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGAT	6	0.15	No Hit
CTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGC	6	0.15	No Hit
CTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTTAG	6	0.15	No Hit
GATGAACCGAACGTAGAGTTAAGGTGCCGGAATACACGCTCATCAGACACC	5	0.125	No Hit
AAAACCCATACGCGTAATGAAAGTGAACGTAGGTTGGGGCCTCGCAAGAGG	5	0.125	No Hit
GATGTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAG	5	0.125	No Hit
GGACCATCGTAATGATTAATAGGGACGGTCGGGGGCATCAGTATTCAATTG	5	0.125	No Hit
CAACTTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGG	5	0.125	No Hit
TGGTAATCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTAATTATT	5	0.125	No Hit
ACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGG	5	0.125	No Hit
CTGATTTTATGCAGGCCACCATCGAAAGGGAATCCGGTTAAGATTCCGGAA	5	0.125	No Hit
CACTACCTTTATAGTTTCTTTACTTATTCAATGAAGCGGAGCTGGAATTCA	5	0.125	No Hit
CAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATACAGTGAAACT	5	0.125	No Hit
CAGTTATCGTTTATTTGATAGTTCCTTTACTACATGGTATAACTGTGGTAA	5	0.125	No Hit
ATTAGATAAAAAATCAATGTCTTCGGACTCTTTGATGATTCATAATAACTT	5	0.125	No Hit
CAGTATTCAATTGTCAGAGGTGAAATTCTTGGATTTATTGAAGACTAACTA	5	0.125	No Hit
CTTAGTGAGGCCTCAGGATCTGCTTAGAGAAGGGGGCAACTCCATCTCAGA	5	0.125	No Hit
CTTTACTTATTCAATGAAGCGGAGCTGGAATTCATTTTCCACGTTCTAGCA	5	0.125	No Hit
CAGATGTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAA	5	0.125	No Hit
CGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGT	5	0.125	No Hit
GTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCG	5	0.125	No Hit
CAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAAT	5	0.125	No Hit
ATGACCTTGACCTATTCTCAAACTTTAAATATGTAAGAAGTCCTTGTTACT	5	0.125	No Hit
GGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAATAATGTAGATAAGGGAA	5	0.125	No Hit
GTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAA	5	0.125	No Hit
GTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGCGGGAGTAACTAT	5	0.125	No Hit
GACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTTTGTGGGTGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 2152654 spots for SRR27822265.sra
Written 2152654 spots for SRR27822265.sra
Read 2152654 spots for SRR27822265.sra
Written 2152654 spots for SRR27822265.sra
Read 2152654 spots for SRR27822265.sra
Written 2152654 spots for SRR27822265.sra
Read 2152655 spots for SRR27822265.sra
Written 2152655 spots for SRR27822265.sra
Read 2152654 spots for SRR27822265.sra
Written 2152654 spots for SRR27822265.sra
Read 2152654 spots for SRR27822265.sra
Written 2152654 spots for SRR27822265.sra
Read 2152654 spots for SRR27822265.sra
Written 2152654 spots for SRR27822265.sra
Read 2152654 spots for SRR27822265.sra
Written 2152654 spots for SRR27822265.sra
SRR ids: ['SRR27822265.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_ekgxjdmw
SRR27822265.sra spots: 17221233
blocks: [[1, 2152654], [2152655, 4305308], [4305309, 6457962], [6457963, 8610616], [8610617, 10763270], [10763271, 12915924], [12915925, 15068578], [15068579, 17221233]]
SRR27822265 file size 2973692
SRR27822265 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR27822265 SRR27822265_1.fastq SRR27822265_2.fastq
Input file:	SRR27822265_1.fastq
Paired file:	SRR27822265_2.fastq
trimmed:	SRR27822265-trimmed-pair1.fastq, SRR27822265-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Thu May 14 08:33:55 2026 >> started

Thu May 14 08:34:12 2026 >> done (16.178s)
17221233 read pairs processed; of these:
     195 ( 0.00%) short read pairs filtered out after trimming by size control
    1511 ( 0.01%) empty read pairs filtered out after trimming by size control
17219527 (99.99%) read pairs available; of these:
   14403 ( 0.08%) trimmed read pairs available after processing
17205124 (99.92%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	      13	  0.00%
 19	     128	  0.00%
 20	      28	  0.00%
 21	      14	  0.00%
 22	      15	  0.00%
 23	      20	  0.00%
 24	      15	  0.00%
 25	      25	  0.00%
 26	      15	  0.00%
 27	      50	  0.00%
 28	      40	  0.00%
 29	      38	  0.00%
 30	      42	  0.00%
 31	      51	  0.00%
 32	      69	  0.00%
 33	      70	  0.00%
 34	     104	  0.00%
 35	     123	  0.00%
 36	     161	  0.00%
 37	     197	  0.00%
 38	     300	  0.00%
 39	     343	  0.00%
 40	     548	  0.00%
 41	     533	  0.00%
 42	     648	  0.00%
 43	     783	  0.00%
 44	     714	  0.00%
 45	     914	  0.01%
 46	     993	  0.01%
 47	    1315	  0.01%
 48	    1625	  0.01%
 49	    2048	  0.01%
 50	    2421	  0.01%
 51	17205124	 99.92%
17219527 reads passed initial QC


criterion=sequence-density
sequence-density=0.27
sequence-density-rank=1
fanout-score=2.58
fanout-score-rank=13
prefix-density=0.25
prefix-fanout=2.6
sequence=ACGCTTGACTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAAAGGATCGATAGGCCACACTTTCATGGTTTGTATTCACACTGAAAATCAAAATCAAGGGGGCTTTTACCCTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCCTTAGGACATCTGCGTTATCGTTT


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=14
fanout-score=33.48
fanout-score-rank=1
prefix-density=0.96
prefix-fanout=1.0
sequence=TCGGGCACCGACGGTACCAGATT


criterion=sequence-density
sequence-density=0.29
sequence-density-rank=1
fanout-score=2.67
fanout-score-rank=16
prefix-density=0.30
prefix-fanout=2.6
sequence=CATAGCGACATTGCTTTTTGATTCTTCGATGTCGGCTCTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGAATGTTACCGCAATAGTAATTGAACTTAGTACGAGAGGAACAGTTCATTCGGATAATTGGTTTTTGCGGCTGTCTGATCAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAATGTCATTTTGCGTGGGGATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=24
fanout-score=84.97
fanout-score-rank=1
prefix-density=0.76
prefix-fanout=1.0
sequence=CATAAACTAAAAATGGCTAGAACTTTCTTTGTCGGTGGTAACTTTAAATTAAACGGTTCCAAACAATCCATTAA
SRR27822265 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 14 08:34:29
                             Started mapping on |	May 14 08:34:29
                                    Finished on |	May 14 08:35:10
       Mapping speed, Million of reads per hour |	1511.96

                          Number of input reads |	17219527
                      Average input read length |	101
                                    UNIQUE READS:
                   Uniquely mapped reads number |	4949696
                        Uniquely mapped reads % |	28.74%
                          Average mapped length |	101.85
                       Number of splices: Total |	86668
            Number of splices: Annotated (sjdb) |	76524
                       Number of splices: GT/AG |	82034
                       Number of splices: GC/AG |	283
                       Number of splices: AT/AC |	88
               Number of splices: Non-canonical |	4263
                      Mismatch rate per base, % |	0.07%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.30
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.08
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	12034958
             % of reads mapped to multiple loci |	69.89%
        Number of reads mapped to too many loci |	123846
             % of reads mapped to too many loci |	0.72%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.64%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	234873	234873	234873
N_multimapping	12034958	12034958	12034958
N_noFeature	196250	4626009	291770
N_ambiguous	275121	2235	50023
UnstrandedReadsAssigned:4478325 PositiveStrandReadsAssigned:321452 NegativeStrandReadsAssigned:4607903
Dataset is classified negative stranded
MeadianReadLen=51 20thPercentileLength=51 echo kmer=47
SRR27822265 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 6,713
[index] number of k-mers: 8,283,195
[index] number of equivalence classes: 8,710
[quant] running in paired-end mode
[quant] will process pair 1: SRR27822265-trimmed-pair1.fastq
                             SRR27822265-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 17,219,527 reads, 5,188,132 reads pseudoaligned
[quant] estimated average fragment length: 190.175
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,001 rounds

  6714 SRR27822265.ke.tsv
  7126 SRR27822265.se.tsv
 13840 total
==> SRR27822265.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
YHR055C	186	60.161	333	339.673
YPR161C	1974	1784.83	448	15.4033
YOL138C	4026	3836.83	535	8.55686
YDR395W	2835	2645.83	1330	30.8477
YGR129W	648	459.601	174	23.2327
YPR165W	630	441.737	1457	202.408
YPR098C	486	301.624	307	62.4604
YPL015C	1074	884.825	406	28.158
YCL050C	966	776.825	895	70.7021

==> SRR27822265.se.tsv <==
YDL248W	68
YDL247W-A	2
YDL247W	7
YDL246C	1
YDL245C	0
YDL244W	12
YDL243C	137
YDL242W	2
YDL241W	35
YDL240C-A	0
SRR27822265 completed mapping pipeline successfully
