Starting /dee2/code/volunteer_pipeline.sh SRR27822243
    current disk space = 186104784
    free memory = 129380548 
SRR27822243 SRAfilesize
766c5174d44e90bb50782eb83e910953  SRR27822243.sra
SRR27822243.sra file validated
SRR27822243 is paired end
SRR27822243 is conventional basespace
SRR27822243 read1 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR27822243_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.364	37.0	37.0	37.0	37.0	37.0
2	36.47	37.0	37.0	37.0	37.0	37.0
3	36.656	37.0	37.0	37.0	37.0	37.0
4	36.664	37.0	37.0	37.0	37.0	37.0
5	36.633	37.0	37.0	37.0	37.0	37.0
6	36.583	37.0	37.0	37.0	37.0	37.0
7	36.667	37.0	37.0	37.0	37.0	37.0
8	36.6615	37.0	37.0	37.0	37.0	37.0
9	36.663	37.0	37.0	37.0	37.0	37.0
10	36.6925	37.0	37.0	37.0	37.0	37.0
11	36.627	37.0	37.0	37.0	37.0	37.0
12	36.64	37.0	37.0	37.0	37.0	37.0
13	36.64	37.0	37.0	37.0	37.0	37.0
14	36.635	37.0	37.0	37.0	37.0	37.0
15	36.6335	37.0	37.0	37.0	37.0	37.0
16	36.6405	37.0	37.0	37.0	37.0	37.0
17	36.5825	37.0	37.0	37.0	37.0	37.0
18	36.5825	37.0	37.0	37.0	37.0	37.0
19	36.647	37.0	37.0	37.0	37.0	37.0
20	36.633	37.0	37.0	37.0	37.0	37.0
21	36.604	37.0	37.0	37.0	37.0	37.0
22	36.573	37.0	37.0	37.0	37.0	37.0
23	36.5505	37.0	37.0	37.0	37.0	37.0
24	36.518	37.0	37.0	37.0	37.0	37.0
25	36.5955	37.0	37.0	37.0	37.0	37.0
26	36.581	37.0	37.0	37.0	37.0	37.0
27	36.599	37.0	37.0	37.0	37.0	37.0
28	36.6635	37.0	37.0	37.0	37.0	37.0
29	36.524	37.0	37.0	37.0	37.0	37.0
30	36.567	37.0	37.0	37.0	37.0	37.0
31	36.6235	37.0	37.0	37.0	37.0	37.0
32	36.58	37.0	37.0	37.0	37.0	37.0
33	36.4875	37.0	37.0	37.0	37.0	37.0
34	36.55	37.0	37.0	37.0	37.0	37.0
35	36.4775	37.0	37.0	37.0	37.0	37.0
36	36.5485	37.0	37.0	37.0	37.0	37.0
37	36.5495	37.0	37.0	37.0	37.0	37.0
38	36.5245	37.0	37.0	37.0	37.0	37.0
39	36.473	37.0	37.0	37.0	37.0	37.0
40	36.517	37.0	37.0	37.0	37.0	37.0
41	36.5535	37.0	37.0	37.0	37.0	37.0
42	36.5035	37.0	37.0	37.0	37.0	37.0
43	36.4065	37.0	37.0	37.0	37.0	37.0
44	36.5405	37.0	37.0	37.0	37.0	37.0
45	36.426	37.0	37.0	37.0	37.0	37.0
46	36.455	37.0	37.0	37.0	37.0	37.0
47	36.444	37.0	37.0	37.0	37.0	37.0
48	36.5335	37.0	37.0	37.0	37.0	37.0
49	36.4815	37.0	37.0	37.0	37.0	37.0
50	36.34	37.0	37.0	37.0	37.0	37.0
51	36.3815	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
26	2.0
27	3.0
28	4.0
29	7.0
30	14.0
31	24.0
32	29.0
33	41.0
34	58.0
35	173.0
36	1816.0
37	1829.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.761569416498993	9.205231388329981	7.168008048289738	60.865191146881294
2	20.150000000000002	12.3	36.1	31.45
3	18.7	15.75	24.8	40.75
4	23.1	24.775	21.175	30.95
5	20.25	31.65	26.400000000000002	21.7
6	18.7	34.625	26.900000000000002	19.775000000000002
7	15.45	20.7	42.225	21.625
8	14.374999999999998	21.725	35.675000000000004	28.225
9	15.6	24.3	36.825	23.275000000000002
10	18.375	35.525	25.674999999999997	20.424999999999997
11	21.975	25.7	22.85	29.475
12	20.175	24.025	29.075	26.724999999999998
13	17.95	23.799999999999997	30.175	28.075
14	18.475	25.025	29.475	27.025
15	18.975	24.125	31.65	25.25
16	19.75	24.7	28.849999999999998	26.700000000000003
17	18.224999999999998	25.55	28.9	27.325
18	20.0	25.174999999999997	29.549999999999997	25.275
19	20.45	25.650000000000002	28.475	25.424999999999997
20	18.65	27.750000000000004	30.425	23.175
21	19.875	28.225	27.975	23.925
22	21.125	26.0	28.375	24.5
23	19.05	27.750000000000004	27.450000000000003	25.75
24	18.175	27.425	27.775	26.625
25	19.15	27.700000000000003	26.450000000000003	26.700000000000003
26	17.45	28.349999999999998	29.65	24.55
27	17.849999999999998	28.499999999999996	27.175	26.474999999999998
28	19.6	27.025	27.200000000000003	26.174999999999997
29	17.75	27.925	30.599999999999998	23.724999999999998
30	17.875	27.025	28.999999999999996	26.1
31	18.099999999999998	26.25	29.45	26.200000000000003
32	21.099999999999998	26.400000000000002	26.674999999999997	25.825
33	19.400000000000002	26.700000000000003	29.5	24.4
34	19.45	27.375	28.000000000000004	25.174999999999997
35	20.0	26.474999999999998	28.975	24.55
36	19.025	26.825	25.974999999999998	28.175
37	16.85	27.675	27.175	28.299999999999997
38	19.5	27.175	28.349999999999998	24.975
39	19.175	28.475	27.3	25.05
40	17.974999999999998	28.299999999999997	28.849999999999998	24.875
41	19.775000000000002	28.125	26.674999999999997	25.424999999999997
42	22.85	27.6	26.724999999999998	22.825
43	22.025	25.275	28.4	24.3
44	21.7	26.0	28.375	23.925
45	22.975	29.049999999999997	24.474999999999998	23.5
46	20.724999999999998	25.35	25.775	28.15
47	21.275	28.599999999999998	26.924999999999997	23.200000000000003
48	21.7	26.275	25.624999999999996	26.400000000000002
49	20.225	27.925	27.675	24.175
50	18.4	29.45	29.049999999999997	23.1
51	20.75	28.7	27.1	23.45
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	2.0
18	4.0
19	4.5
20	5.0
21	5.0
22	5.0
23	6.5
24	8.0
25	12.0
26	20.0
27	24.0
28	35.0
29	46.0
30	64.0
31	82.0
32	121.0
33	160.0
34	168.5
35	177.0
36	195.5
37	214.0
38	248.0
39	282.0
40	304.0
41	326.0
42	320.0
43	314.0
44	325.0
45	336.0
46	337.5
47	339.0
48	362.0
49	385.0
50	350.0
51	315.0
52	299.5
53	284.0
54	283.0
55	282.0
56	212.0
57	142.0
58	122.5
59	103.0
60	88.5
61	74.0
62	54.0
63	34.0
64	24.0
65	14.0
66	15.5
67	17.0
68	13.5
69	10.0
70	5.5
71	1.0
72	0.5
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.6
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.824999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.59548683660677	45.225
2	12.327622231508567	14.75
3	4.471374843292938	8.025
4	2.0058503969912245	4.8
5	1.420810697868784	4.25
6	1.0447137484329294	3.75
7	1.170079398244881	4.9
8	0.5014625992478061	2.4
9	0.25073129962390306	1.35
>10	1.2118679481821981	10.549999999999999
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAAT	27	0.675	No Hit
CCTGTGGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAAAG	26	0.65	No Hit
CGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCA	23	0.575	No Hit
CGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAAATTCTCCGCTCTGA	22	0.5499999999999999	No Hit
CTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATAAC	21	0.525	No Hit
ACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAAATTCTCCGCTCTG	18	0.44999999999999996	No Hit
CCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGGAAC	17	0.42500000000000004	No Hit
CTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCT	16	0.4	No Hit
CGCTTGACTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGGCACCTC	15	0.375	No Hit
GTACTTTTTCACTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTAC	15	0.375	No Hit
CCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCCGAA	14	0.35000000000000003	No Hit
CCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCCGA	13	0.325	No Hit
CCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTAAAAGCTCTTCAT	13	0.325	No Hit
CTCGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAACG	13	0.325	No Hit
CTAGAATTACCACAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGA	13	0.325	No Hit
GTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAG	13	0.325	No Hit
AAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCC	13	0.325	No Hit
CTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTATCG	12	0.3	No Hit
CTCGTACTAAGTTCAATTACTATTGCGGTAACATTCATCAGTAGGGTAAAA	12	0.3	No Hit
CTCCAATTGTTCCTCGTTAAGGTATTTACATTGTACTCATTCCAATTACAA	12	0.3	No Hit
CCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTA	11	0.27499999999999997	No Hit
TGACAATTGAATACTGATGCCCCCGACCGTCCCTATTAATCATTACGATGG	11	0.27499999999999997	No Hit
CTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTTC	11	0.27499999999999997	No Hit
CACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGCTT	11	0.27499999999999997	No Hit
CCCAAAGTTGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATT	10	0.25	No Hit
CTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAAATTCTCCGCTCTGAGAT	10	0.25	No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTT	10	0.25	No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCCGACTTCCC	10	0.25	No Hit
CCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAA	10	0.25	No Hit
CCCACTAAAAGCTCTTCATTCAAATGTCCACGTTCAATTAAGTAACAAGGA	9	0.22499999999999998	No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTG	9	0.22499999999999998	No Hit
CACCAACTAAGAACGGCCATGCACCACCACCCACAAAATCAAGAAAGAGCT	9	0.22499999999999998	No Hit
CTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAA	9	0.22499999999999998	No Hit
CGGTAACATTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAA	9	0.22499999999999998	No Hit
GCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAA	9	0.22499999999999998	No Hit
CCCAGCACGACGGAGTTTCACAAGATTACCAAGACCTCTCGGCCAAGGTTA	8	0.2	No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCA	8	0.2	No Hit
CCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGT	8	0.2	No Hit
CTCTTACTCAAATCCATCCGAAGACATCAGGATCGGTCGATTGTGCACCTC	8	0.2	No Hit
GTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAGGTGCTGG	8	0.2	No Hit
CTGTGGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAAAGG	8	0.2	No Hit
CCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTT	8	0.2	No Hit
CGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAACGGC	8	0.2	No Hit
CGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTTCGCCA	8	0.2	No Hit
CCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGGCACCTCTAGCCTCAAA	8	0.2	No Hit
ATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGC	8	0.2	No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTG	8	0.2	No Hit
CTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAGTTT	7	0.17500000000000002	No Hit
GTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAACGGCC	7	0.17500000000000002	No Hit
CCACACTTTCATGGTTTGTATTCACACTGAAAATCAAAATCAAGGGGGCTT	7	0.17500000000000002	No Hit
CTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCT	7	0.17500000000000002	No Hit
CCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTG	7	0.17500000000000002	No Hit
GTAACATTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACC	7	0.17500000000000002	No Hit
CCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAAA	7	0.17500000000000002	No Hit
CCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTATTCTAT	7	0.17500000000000002	No Hit
CCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAG	7	0.17500000000000002	No Hit
CTTTTTCACTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTG	7	0.17500000000000002	No Hit
GCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTTCGCCAA	7	0.17500000000000002	No Hit
AGCATGTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAAC	7	0.17500000000000002	No Hit
CGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAG	7	0.17500000000000002	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCA	7	0.17500000000000002	No Hit
CCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTC	7	0.17500000000000002	No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCA	7	0.17500000000000002	No Hit
CTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGGG	7	0.17500000000000002	No Hit
CATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGAT	7	0.17500000000000002	No Hit
CTCATTCCAATTACAAGACCCGAATGGGCCCTGTATCGTTATTTATTGTCA	7	0.17500000000000002	No Hit
CGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCA	7	0.17500000000000002	No Hit
GTCGCAGTCCTCAGTCCCAGCTGGCAGTATTCCCACAGGCTATAATACTTA	7	0.17500000000000002	No Hit
GCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTTG	7	0.17500000000000002	No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCAGAA	7	0.17500000000000002	No Hit
CCCAGTGAAATGCGAGATTCCCCTACCCACAAGGAGCAGAGGGCACAAAAC	7	0.17500000000000002	No Hit
AGCGTGTATTCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCA	7	0.17500000000000002	No Hit
CTAAAGGATCGATAGGCCACACTTTCATGGTTTGTATTCACACTGAAAATC	7	0.17500000000000002	No Hit
CAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCCGAATG	7	0.17500000000000002	No Hit
CTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAAATTCTCC	7	0.17500000000000002	No Hit
GCTAGTTCATTCGGCCGGTGAGTTGTTACACACTCCTTAGCGGATTCCGAC	6	0.15	No Hit
CCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATAA	6	0.15	No Hit
CCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCC	6	0.15	No Hit
CGATAGGCCACACTTTCATGGTTTGTATTCACACTGAAAATCAAAATCAAG	6	0.15	No Hit
CCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAGC	6	0.15	No Hit
GTGGTATTTCACTGGCGCCGAAGCTCCCACTTATTCTACACCCTCTATGTC	6	0.15	No Hit
GTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACC	6	0.15	No Hit
GCATGTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAACT	6	0.15	No Hit
GCCACACTTTCATGGTTTGTATTCACACTGAAAATCAAAATCAAGGGGGCT	6	0.15	No Hit
CCTCAGTCCCAGCTGGCAGTATTCCCACAGGCTATAATACTTACCGAGGCA	6	0.15	No Hit
CTGCCACCAAAACTGATGCTGGCCCAGTGAAATGCGAGATTCCCCTACCCA	6	0.15	No Hit
CGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAG	6	0.15	No Hit
GTCTGATCAAATGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCT	6	0.15	No Hit
CCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAAGAAT	6	0.15	No Hit
CTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTTCGCCAAGA	6	0.15	No Hit
CGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTTATACCATGTA	6	0.15	No Hit
CTTCATTCAAATGTCCACGTTCAATTAAGTAACAAGGACTTCTTACATATT	6	0.15	No Hit
CCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAA	6	0.15	No Hit
CCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAGCCT	6	0.15	No Hit
CGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCA	6	0.15	No Hit
GCCCAGTGAAATGCGAGATTCCCCTACCCACAAGGAGCAGAGGGCACAAAA	6	0.15	No Hit
CTCGCTGGCTCCGTCAGTGTAGCGCGCGTGCGGCCCAGAACGTCTAAGGGC	6	0.15	No Hit
GTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTA	6	0.15	No Hit
CTCCGGAATCGAACCCTTATTCCCCGTTACCCGTTGAAACCATGGTAGGCC	6	0.15	No Hit
GGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAAAGGATCG	6	0.15	No Hit
CCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTCGCA	5	0.125	No Hit
CTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTCGCAG	5	0.125	No Hit
GTCGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTTATACCATG	5	0.125	No Hit
GTCCTCAGTCCCAGCTGGCAGTATTCCCACAGGCTATAATACTTACCGAGG	5	0.125	No Hit
CCGCTACGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCAT	5	0.125	No Hit
CCAACAGCTATGCTCTTACTCAAATCCATCCGAAGACATCAGGATCGGTCG	5	0.125	No Hit
CCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTG	5	0.125	No Hit
CGAAGACATTGATTTTTTATCTAATAAATACATCTCTTCCAAAGGGTCGAG	5	0.125	No Hit
CTCCTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGA	5	0.125	No Hit
CACCTGACAATGTCTTCAACCCGGATCAGCCCCGAATGGGACCTTGAATGC	5	0.125	No Hit
GTGGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAAAGGAT	5	0.125	No Hit
CGCTACGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATA	5	0.125	No Hit
CCGCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTGACATTCAGAGCA	5	0.125	No Hit
GTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCAGAACCCAA	5	0.125	No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGG	5	0.125	No Hit
CCTCACTACCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTA	5	0.125	No Hit
CTAGAAACCAACAAAATAGAACCAAACGTCCTATTCTATTATTCCATGCTA	5	0.125	No Hit
CCGGAATCGAACCCTTATTCCCCGTTACCCGTTGAAACCATGGTAGGCCAC	5	0.125	No Hit
CGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTAC	5	0.125	No Hit
CTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTGGTTGAATTT	5	0.125	No Hit
CCTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTT	5	0.125	No Hit
CACTACCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTT	5	0.125	No Hit
CCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTTCGCCAAG	5	0.125	No Hit
CTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAAGAATCCATATC	5	0.125	No Hit
GTCCCAACAGCTATGCTCTTACTCAAATCCATCCGAAGACATCAGGATCGG	5	0.125	No Hit
CCTGTTATTGCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAG	5	0.125	No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGC	5	0.125	No Hit
CTGACAATTGAATACTGATGCCCCCGACCGTCCCTATTAATCATTACGATG	5	0.125	No Hit
CGGAAACCTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAA	5	0.125	No Hit
CCTGAATTAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGG	5	0.125	No Hit
CCCCAAAGTTGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGAT	5	0.125	No Hit
CACACTTTCATGGTTTGTATTCACACTGAAAATCAAAATCAAGGGGGCTTT	5	0.125	No Hit
CTGCTATCCTGAGGGAAACTTCGGCAGGAACCAGCTACTAGATGGTTCGAT	5	0.125	No Hit
CGGGTCCCAACAGCTATGCTCTTACTCAAATCCATCCGAAGACATCAGGAT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR27822243 read2 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR27822243_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.38875	37.0	37.0	37.0	37.0	37.0
2	36.207	37.0	37.0	37.0	37.0	37.0
3	36.3695	37.0	37.0	37.0	37.0	37.0
4	36.25	37.0	37.0	37.0	37.0	37.0
5	36.29625	37.0	37.0	37.0	37.0	37.0
6	36.233	37.0	37.0	37.0	37.0	37.0
7	36.228	37.0	37.0	37.0	37.0	37.0
8	36.32275	37.0	37.0	37.0	37.0	37.0
9	36.51	37.0	37.0	37.0	37.0	37.0
10	36.35325	37.0	37.0	37.0	37.0	37.0
11	36.426	37.0	37.0	37.0	37.0	37.0
12	36.38475	37.0	37.0	37.0	37.0	37.0
13	36.307	37.0	37.0	37.0	37.0	37.0
14	36.364	37.0	37.0	37.0	37.0	37.0
15	36.28975	37.0	37.0	37.0	37.0	37.0
16	36.4705	37.0	37.0	37.0	37.0	37.0
17	36.3585	37.0	37.0	37.0	37.0	37.0
18	36.32025	37.0	37.0	37.0	37.0	37.0
19	36.326	37.0	37.0	37.0	37.0	37.0
20	36.43975	37.0	37.0	37.0	37.0	37.0
21	36.3	37.0	37.0	37.0	37.0	37.0
22	36.29175	37.0	37.0	37.0	37.0	37.0
23	36.4195	37.0	37.0	37.0	37.0	37.0
24	36.3785	37.0	37.0	37.0	37.0	37.0
25	36.3225	37.0	37.0	37.0	37.0	37.0
26	36.421	37.0	37.0	37.0	37.0	37.0
27	36.336	37.0	37.0	37.0	37.0	37.0
28	36.286	37.0	37.0	37.0	37.0	37.0
29	36.3505	37.0	37.0	37.0	37.0	37.0
30	36.21875	37.0	37.0	37.0	37.0	37.0
31	36.16625	37.0	37.0	37.0	37.0	37.0
32	36.185	37.0	37.0	37.0	37.0	37.0
33	36.26475	37.0	37.0	37.0	37.0	37.0
34	36.11525	37.0	37.0	37.0	37.0	37.0
35	36.2485	37.0	37.0	37.0	37.0	37.0
36	36.37425	37.0	37.0	37.0	37.0	37.0
37	36.219	37.0	37.0	37.0	37.0	37.0
38	36.31575	37.0	37.0	37.0	37.0	37.0
39	36.111	37.0	37.0	37.0	37.0	37.0
40	36.21225	37.0	37.0	37.0	37.0	37.0
41	36.20875	37.0	37.0	37.0	37.0	37.0
42	36.16425	37.0	37.0	37.0	37.0	37.0
43	36.179	37.0	37.0	37.0	37.0	37.0
44	36.05375	37.0	37.0	37.0	37.0	37.0
45	36.27025	37.0	37.0	37.0	37.0	37.0
46	36.09675	37.0	37.0	37.0	37.0	37.0
47	36.122	37.0	37.0	37.0	37.0	37.0
48	36.15125	37.0	37.0	37.0	37.0	37.0
49	36.18275	37.0	37.0	37.0	37.0	37.0
50	36.05725	37.0	37.0	37.0	37.0	37.0
51	36.12475	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	3.0
21	4.0
22	4.0
23	1.0
24	1.0
25	5.0
26	1.0
27	5.0
28	6.0
29	11.0
30	14.0
31	20.0
32	23.0
33	43.0
34	102.0
35	438.0
36	2181.0
37	1136.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.821616212159118	18.689016762571928	12.884663497623217	39.60470352764573
2	27.400000000000002	21.7	35.9	15.0
3	19.650000000000002	27.6	30.45	22.3
4	23.0	34.175	24.125	18.7
5	25.39404553415061	36.10207655741806	22.166624968726545	16.337252939704776
6	22.900000000000002	37.1	22.325	17.675
7	21.325	21.75	37.475	19.45
8	23.592694520890667	21.766324743557668	31.873905429071804	22.76707530647986
9	24.775	24.349999999999998	30.525000000000002	20.349999999999998
10	21.841381035776834	36.82762071553665	22.641981486114584	18.689016762571928
11	27.85	28.225	22.0	21.925
12	28.021015761821367	24.218163622717036	25.39404553415061	22.366775081310983
13	25.63781890945473	25.76288144072036	26.3631815907954	22.236118059029515
14	26.05	27.750000000000004	27.925	18.275
15	22.641981486114584	30.848136102076555	27.745809357017766	18.764073054791094
16	26.150000000000002	26.924999999999997	27.1	19.825
17	25.85	30.375000000000004	24.6	19.175
18	27.195396547410557	27.47060295221416	25.143857893420062	20.190142606955217
19	24.5	27.025	26.474999999999998	22.0
20	24.26820115086315	32.399299474605954	25.469101826369776	17.86339754816112
21	23.95	30.075000000000003	28.575	17.4
22	26.25656414103526	28.157039259814955	27.00675168792198	18.579644911227806
23	25.3	29.4	27.450000000000003	17.849999999999998
24	25.025	30.575000000000003	24.975	19.425
25	24.875	28.7	27.0	19.425
26	26.125	30.575000000000003	25.575	17.724999999999998
27	24.8	30.675	27.150000000000002	17.375
28	25.424999999999997	29.825000000000003	26.724999999999998	18.025
29	25.6	30.8	25.5	18.099999999999998
30	25.731432858214554	28.80720180045011	26.03150787696924	19.42985746436609
31	25.55638909727432	29.15728932233058	25.35633908477119	19.929982495623904
32	24.975	29.049999999999997	25.15	20.825
33	25.344008006004504	30.447835876907682	25.268951713785338	18.939204403302476
34	24.818613960470355	30.322742056542406	25.769326995246434	19.089316987740805
35	25.224999999999998	31.474999999999998	24.975	18.325
36	25.756439109777446	27.406851712928233	27.656914228557138	19.179794948737182
37	25.224999999999998	28.325	27.075	19.375
38	26.1195896922692	28.596447335501622	26.770077558168627	18.513885414060546
39	26.450000000000003	30.825000000000003	23.799999999999997	18.925
40	23.767825869402053	29.947460595446586	26.09457092819615	20.190142606955217
41	26.845133850387793	27.89592194145609	26.970227670753065	18.28871653740305
42	24.18104526131533	27.956989247311824	26.206551637909474	21.655413853463365
43	24.725	29.75	26.200000000000003	19.325
44	22.992244183137352	30.34776082061546	25.769326995246434	20.89066800100075
45	23.967975981986488	30.2727045283963	27.745809357017766	18.01351013259945
46	25.8443832874656	29.39704778583938	26.21966474856142	18.5389041781336
47	24.425	28.575	27.875	19.125
48	25.344008006004504	28.646484863647736	26.069552164123095	19.93995496622467
49	23.792844633475106	29.82236677508131	27.695771828871653	18.689016762571928
50	25.70642660665166	28.107026756689173	27.38184546136534	18.804701175293822
51	25.369026770077557	29.797348011008257	27.570678008506377	17.262947210407805
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	1.0
16	1.0
17	1.5
18	2.0
19	3.5
20	5.0
21	5.5
22	6.0
23	7.0
24	8.0
25	11.5
26	27.0
27	39.0
28	50.5
29	62.0
30	92.0
31	122.0
32	115.0
33	108.0
34	149.0
35	190.0
36	222.0
37	254.0
38	266.5
39	279.0
40	316.0
41	353.0
42	402.5
43	452.0
44	463.5
45	475.0
46	418.5
47	362.0
48	340.5
49	319.0
50	290.0
51	261.0
52	229.5
53	198.0
54	174.0
55	150.0
56	147.0
57	144.0
58	118.5
59	93.0
60	65.0
61	37.0
62	34.0
63	31.0
64	22.0
65	13.0
66	14.0
67	15.0
68	8.5
69	2.0
70	1.0
71	0.0
72	0.0
73	0.0
74	0.5
75	0.5
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.5
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.075
2	0.0
3	0.0
4	0.0
5	0.075
6	0.0
7	0.0
8	0.075
9	0.0
10	0.075
11	0.0
12	0.075
13	0.05
14	0.0
15	0.075
16	0.0
17	0.0
18	0.075
19	0.0
20	0.075
21	0.0
22	0.025
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.025
31	0.025
32	0.0
33	0.075
34	0.075
35	0.0
36	0.025
37	0.0
38	0.075
39	0.0
40	0.075
41	0.075
42	0.025
43	0.0
44	0.075
45	0.075
46	0.075
47	0.0
48	0.075
49	0.075
50	0.025
51	0.075
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.5
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.61068702290076	49.525000000000006
2	13.702290076335878	17.95
3	5.076335877862595	9.975000000000001
4	2.4427480916030535	6.4
5	1.3358778625954197	4.375
6	0.6106870229007634	2.4
7	0.26717557251908397	1.225
8	0.15267175572519084	0.8
9	0.11450381679389314	0.675
>10	0.6870229007633588	6.675000000000001
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTG	35	0.8750000000000001	No Hit
CAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA	20	0.5	No Hit
CTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAGT	19	0.475	No Hit
CGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATT	19	0.475	No Hit
CCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAA	16	0.4	No Hit
CTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAAA	15	0.375	No Hit
GTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAA	15	0.375	No Hit
CACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGAT	15	0.375	No Hit
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGA	14	0.35000000000000003	No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG	13	0.325	No Hit
GGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAAAAGCCCCCTTGATTTT	13	0.325	No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGT	12	0.3	No Hit
ATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAG	11	0.27499999999999997	No Hit
GTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTTAACCTACTAAAT	10	0.25	No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT	10	0.25	No Hit
CTTGGATTTATTGAAGACTAACTACTGCGAAAGCATTTGCCAAGGACGTTT	10	0.25	No Hit
ACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTT	10	0.25	No Hit
CGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAGTGAAC	10	0.25	No Hit
ACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATT	9	0.22499999999999998	No Hit
GTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGA	9	0.22499999999999998	No Hit
TCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG	9	0.22499999999999998	No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGT	8	0.2	No Hit
GTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAA	8	0.2	No Hit
GGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATG	8	0.2	No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACG	8	0.2	No Hit
GTAGAATAAGTGGGAGCTTCGGCGCCAGTGAAATACCACTACCTTTATAGT	7	0.17500000000000002	No Hit
CTGATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAATA	7	0.17500000000000002	No Hit
GGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAA	7	0.17500000000000002	No Hit
CACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTGT	7	0.17500000000000002	No Hit
CAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCTA	7	0.17500000000000002	No Hit
CGGAATTTGAGGCTAGAGGTGCCAGAAAAGTTACCACAGGGATAACTGGCT	7	0.17500000000000002	No Hit
ATGACCTTGACCTATTCTCAAACTTTAAATATGTAAGAAGTCCTTGTTACT	7	0.17500000000000002	No Hit
CTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAGCG	6	0.15	No Hit
CAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGT	6	0.15	No Hit
AGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAA	6	0.15	No Hit
CTTAACCATAAACTATGCCGACTAGGGATCGGGTGGTGTTTTTTTAATGAC	6	0.15	No Hit
CCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAG	6	0.15	No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAT	6	0.15	No Hit
GTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGT	6	0.15	No Hit
AGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACC	6	0.15	No Hit
CGGCTCTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCA	6	0.15	No Hit
GGCTTAGTGAGGCCTCAGGATCTGCTTAGAGAAGGGGGCAACTCCATCTCA	6	0.15	No Hit
CTTTTCGAATCGCATGGCCTTGTGCTGGCGATGGTTCATTCAAATTTCTGC	6	0.15	No Hit
CGAGACCTTAACCTACTAAATAGTGGTGCTAGCATTTGCTGGTTATCCACT	6	0.15	No Hit
GTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAA	6	0.15	No Hit
GACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTTTGTGGGTGG	6	0.15	No Hit
ATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTATGCCT	6	0.15	No Hit
GTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGT	6	0.15	No Hit
CTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATACAGTGAAA	5	0.125	No Hit
CGATCGTCGAATTTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTA	5	0.125	No Hit
GGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAAAAGCC	5	0.125	No Hit
CGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTT	5	0.125	No Hit
GATTAAGCCATGCATGTCTAAGTATAAGCAATTTATACAGTGAAACTGCGA	5	0.125	No Hit
TGGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAAAAGCCCCCTTGATTT	5	0.125	No Hit
CCGAAAGATGGTGAACTATGCCTGAATAGGGTGAAGCCAGAGGAAACTCTG	5	0.125	No Hit
CGTCGAATTTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTG	5	0.125	No Hit
CCAAGGTGAACAGCCTCTAGTTGATAGAATAATGTAGATAAGGGAAGTCGG	5	0.125	No Hit
CCGCAATAGTAATTGAACTTAGTACGAGAGGAACAGTTCATTCGGATAATT	5	0.125	No Hit
GTTTTCATGCCAGGTCGTACTGATAACCGCAGCAGGTCTCCAAGGTGAACA	5	0.125	No Hit
GCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGGTTCTGACGTGCAAATCGA	5	0.125	No Hit
CGCTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAGAA	5	0.125	No Hit
GCCTTAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTA	5	0.125	No Hit
CAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGGT	5	0.125	No Hit
CAACTTTCGATGGTAGGATAGTGGCCTACCATGGTTTCAACGGGTAACGGG	5	0.125	No Hit
GACTAACTACTGCGAAAGCATTTGCCAAGGACGTTTTCATTAATCAAGAAC	5	0.125	No Hit
CTCAGGATAGCAGAAGCTCGTATCAGTTTTATGAGGTAAAGCGAATGATTA	5	0.125	No Hit
CTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTTAG	5	0.125	No Hit
TGAAAGTGAACGTAGGTTGGGGCCTCGCAAGAGGTGCACAATCGACCGATC	5	0.125	No Hit
CAGTATTCAATTGTCAGAGGTGAAATTCTTGGATTTATTGAAGACTAACTA	5	0.125	No Hit
ATTGGAATGAGTACAATGTAAATACCTTAACGAGGAACAATTGGAGGGCAA	5	0.125	No Hit
ATTGGCTCTAAGGGTCGGGTAGTGAGGGCCTTGGTCAGACGCAGCGGGCGT	5	0.125	No Hit
CTTTTTTTATACTGAGCGTATTGGAACGTTATCGATAAGAAGAGAGCGTCT	5	0.125	No Hit
CAGATGTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAA	5	0.125	No Hit
CAAAAGGGTAAAAGCCCCCTTGATTTTGATTTTCAGTGTGAATACAAACCA	5	0.125	No Hit
GTGAAATACCACTACCTTTATAGTTTCTTTACTTATTCAATGAAGCGGAGC	5	0.125	No Hit
GAACAGTTCATTCGGATAATTGGTTTTTGCGGCTGTCTGATCAGGCATTGC	5	0.125	No Hit
CTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGT	5	0.125	No Hit
CAGTGACGAAGCCTAGACCGTAAGGTCGGGTCGAACGGCCTCTAGTGCAGA	5	0.125	No Hit
CTCACCGGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTT	5	0.125	No Hit
CTCGCAAGAGGTGCACAATCGACCGATCCTGATGTCTTCGGATGGATTTGA	5	0.125	No Hit
GTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGG	5	0.125	No Hit
TCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACTA	5	0.125	No Hit
CGAAAGATGGTGAACTATGCCTGAATAGGGTGAAGCCAGAGGAAACTCTGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 1941324 spots for SRR27822243.sra
Written 1941324 spots for SRR27822243.sra
Read 1941324 spots for SRR27822243.sra
Written 1941324 spots for SRR27822243.sra
Read 1941324 spots for SRR27822243.sra
Written 1941324 spots for SRR27822243.sra
Read 1941324 spots for SRR27822243.sra
Written 1941324 spots for SRR27822243.sra
Read 1941326 spots for SRR27822243.sra
Written 1941326 spots for SRR27822243.sra
Read 1941324 spots for SRR27822243.sra
Written 1941324 spots for SRR27822243.sra
Read 1941324 spots for SRR27822243.sra
Written 1941324 spots for SRR27822243.sra
Read 1941324 spots for SRR27822243.sra
Written 1941324 spots for SRR27822243.sra
SRR ids: ['SRR27822243.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd__30kddhg
SRR27822243.sra spots: 15530594
blocks: [[1, 1941324], [1941325, 3882648], [3882649, 5823972], [5823973, 7765296], [7765297, 9706620], [9706621, 11647944], [11647945, 13589268], [13589269, 15530594]]
SRR27822243 file size 2680696
SRR27822243 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR27822243 SRR27822243_1.fastq SRR27822243_2.fastq
Input file:	SRR27822243_1.fastq
Paired file:	SRR27822243_2.fastq
trimmed:	SRR27822243-trimmed-pair1.fastq, SRR27822243-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Thu May 14 09:18:05 2026 >> started

Thu May 14 09:19:57 2026 >> done (111.128s)
15530594 read pairs processed; of these:
     146 ( 0.00%) short read pairs filtered out after trimming by size control
     946 ( 0.01%) empty read pairs filtered out after trimming by size control
15529502 (99.99%) read pairs available; of these:
    8030 ( 0.05%) trimmed read pairs available after processing
15521472 (99.95%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	      30	  0.00%
 19	     107	  0.00%
 20	      19	  0.00%
 21	      10	  0.00%
 22	      14	  0.00%
 23	      12	  0.00%
 24	      14	  0.00%
 25	      19	  0.00%
 26	       7	  0.00%
 27	      37	  0.00%
 28	      19	  0.00%
 29	      29	  0.00%
 30	      34	  0.00%
 31	      27	  0.00%
 32	      47	  0.00%
 33	      48	  0.00%
 34	      76	  0.00%
 35	      88	  0.00%
 36	     113	  0.00%
 37	     148	  0.00%
 38	     199	  0.00%
 39	     229	  0.00%
 40	     265	  0.00%
 41	     305	  0.00%
 42	     369	  0.00%
 43	     427	  0.00%
 44	     441	  0.00%
 45	     529	  0.00%
 46	     584	  0.00%
 47	     677	  0.00%
 48	     941	  0.01%
 49	     981	  0.01%
 50	    1185	  0.01%
 51	15521472	 99.95%
15529502 reads passed initial QC


criterion=sequence-density
sequence-density=0.27
sequence-density-rank=1
fanout-score=2.41
fanout-score-rank=10
prefix-density=0.26
prefix-fanout=2.4
sequence=ACGCTTGACTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAAAGGATCGATAGGCCACACTTTCATGGTTTGTATTCACACTGAAAATCAAAATCAAGGGGGCTTTTACCCTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCCTTAGGACATCTGCGTTATCGTTT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=35
fanout-score=72.24
fanout-score-rank=1
prefix-density=0.41
prefix-fanout=1.0
sequence=TCCAATTGTTCGTGATGCATTTCAACGGACTTGACTTCAGTGGTAACACCAGCTGGGGC


criterion=sequence-density
sequence-density=0.31
sequence-density-rank=1
fanout-score=2.59
fanout-score-rank=20
prefix-density=0.32
prefix-fanout=2.5
sequence=CATAGCGACATTGCTTTTTGATTCTTCGATGTCGGCTCTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGAATGTTACCGCAATAGTAATTGAACTTAGTACGAGAGGAACAGTTCATTCGGATAATTGGTTTTTGCGGCTGTCTGATCAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAATGTCATTTTGCGTGGGGATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTAG


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=25
fanout-score=82.51
fanout-score-rank=1
prefix-density=0.72
prefix-fanout=1.0
sequence=CATAAACTAAAAATGGCTAGAACTTTCTTTGTCGGTGGTAACTTTAAATTAAACGGTTCCAAACAATCCATTA
SRR27822243 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 14 09:20:26
                             Started mapping on |	May 14 09:20:26
                                    Finished on |	May 14 09:20:47
       Mapping speed, Million of reads per hour |	2662.20

                          Number of input reads |	15529502
                      Average input read length |	101
                                    UNIQUE READS:
                   Uniquely mapped reads number |	4279056
                        Uniquely mapped reads % |	27.55%
                          Average mapped length |	101.86
                       Number of splices: Total |	91361
            Number of splices: Annotated (sjdb) |	82301
                       Number of splices: GT/AG |	87660
                       Number of splices: GC/AG |	175
                       Number of splices: AT/AC |	67
               Number of splices: Non-canonical |	3459
                      Mismatch rate per base, % |	0.07%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.29
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.08
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	11081116
             % of reads mapped to multiple loci |	71.36%
        Number of reads mapped to too many loci |	102269
             % of reads mapped to too many loci |	0.66%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.43%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	169330	169330	169330
N_multimapping	11081116	11081116	11081116
N_noFeature	158053	3989059	243272
N_ambiguous	238287	1775	36304
UnstrandedReadsAssigned:3882716 PositiveStrandReadsAssigned:288222 NegativeStrandReadsAssigned:3999480
Dataset is classified negative stranded
MeadianReadLen=51 20thPercentileLength=51 echo kmer=47
SRR27822243 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 6,713
[index] number of k-mers: 8,283,195
[index] number of equivalence classes: 8,710
[quant] running in paired-end mode
[quant] will process pair 1: SRR27822243-trimmed-pair1.fastq
                             SRR27822243-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 15,529,502 reads, 4,549,999 reads pseudoaligned
[quant] estimated average fragment length: 198.425
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 924 rounds

  6714 SRR27822243.ke.tsv
  7126 SRR27822243.se.tsv
 13840 total
==> SRR27822243.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
YHR055C	186	56.0892	335.5	360.52
YPR161C	1974	1776.57	436	14.7917
YOL138C	4026	3828.57	409	6.43876
YDR395W	2835	2637.57	1370	31.3063
YGR129W	648	451.358	117	15.6236
YPR165W	630	433.565	1067	148.329
YPR098C	486	293.67	128	26.2704
YPL015C	1074	876.575	360	24.7531
YCL050C	966	768.575	1060	83.1258

==> SRR27822243.se.tsv <==
YDL248W	41
YDL247W-A	0
YDL247W	3
YDL246C	0
YDL245C	0
YDL244W	11
YDL243C	120
YDL242W	2
YDL241W	42
YDL240C-A	0
SRR27822243 completed mapping pipeline successfully
