Starting /dee2/code/volunteer_pipeline.sh SRR27822276
    current disk space = 182883472
    free memory = 129410316 
SRR27822276 SRAfilesize
06794446142ebb43044d7f1603002f41  SRR27822276.sra
SRR27822276.sra file validated
SRR27822276 is paired end
SRR27822276 is conventional basespace
SRR27822276 read1 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR27822276_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.4575	37.0	37.0	37.0	37.0	37.0
2	36.5875	37.0	37.0	37.0	37.0	37.0
3	36.6735	37.0	37.0	37.0	37.0	37.0
4	36.69	37.0	37.0	37.0	37.0	37.0
5	36.581	37.0	37.0	37.0	37.0	37.0
6	36.5925	37.0	37.0	37.0	37.0	37.0
7	36.549	37.0	37.0	37.0	37.0	37.0
8	36.5745	37.0	37.0	37.0	37.0	37.0
9	36.52	37.0	37.0	37.0	37.0	37.0
10	36.5995	37.0	37.0	37.0	37.0	37.0
11	36.715	37.0	37.0	37.0	37.0	37.0
12	36.6275	37.0	37.0	37.0	37.0	37.0
13	36.665	37.0	37.0	37.0	37.0	37.0
14	36.6705	37.0	37.0	37.0	37.0	37.0
15	36.542	37.0	37.0	37.0	37.0	37.0
16	36.5855	37.0	37.0	37.0	37.0	37.0
17	36.6495	37.0	37.0	37.0	37.0	37.0
18	36.558	37.0	37.0	37.0	37.0	37.0
19	36.6345	37.0	37.0	37.0	37.0	37.0
20	36.6255	37.0	37.0	37.0	37.0	37.0
21	36.6095	37.0	37.0	37.0	37.0	37.0
22	36.6095	37.0	37.0	37.0	37.0	37.0
23	36.609	37.0	37.0	37.0	37.0	37.0
24	36.572	37.0	37.0	37.0	37.0	37.0
25	36.639	37.0	37.0	37.0	37.0	37.0
26	36.552	37.0	37.0	37.0	37.0	37.0
27	36.584	37.0	37.0	37.0	37.0	37.0
28	36.5215	37.0	37.0	37.0	37.0	37.0
29	36.5185	37.0	37.0	37.0	37.0	37.0
30	36.476	37.0	37.0	37.0	37.0	37.0
31	36.5935	37.0	37.0	37.0	37.0	37.0
32	36.5365	37.0	37.0	37.0	37.0	37.0
33	36.4675	37.0	37.0	37.0	37.0	37.0
34	36.516	37.0	37.0	37.0	37.0	37.0
35	36.5125	37.0	37.0	37.0	37.0	37.0
36	36.4475	37.0	37.0	37.0	37.0	37.0
37	36.543	37.0	37.0	37.0	37.0	37.0
38	36.487	37.0	37.0	37.0	37.0	37.0
39	36.4915	37.0	37.0	37.0	37.0	37.0
40	36.4605	37.0	37.0	37.0	37.0	37.0
41	36.4745	37.0	37.0	37.0	37.0	37.0
42	36.3285	37.0	37.0	37.0	37.0	37.0
43	36.381	37.0	37.0	37.0	37.0	37.0
44	36.3905	37.0	37.0	37.0	37.0	37.0
45	36.476	37.0	37.0	37.0	37.0	37.0
46	36.4785	37.0	37.0	37.0	37.0	37.0
47	36.446	37.0	37.0	37.0	37.0	37.0
48	36.457	37.0	37.0	37.0	37.0	37.0
49	36.4725	37.0	37.0	37.0	37.0	37.0
50	36.4325	37.0	37.0	37.0	37.0	37.0
51	36.443	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	0.0
25	0.0
26	1.0
27	2.0
28	7.0
29	7.0
30	16.0
31	16.0
32	29.0
33	49.0
34	62.0
35	209.0
36	1763.0
37	1838.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.733065730055195	8.73055694932263	7.827395885599599	59.70898143502258
2	18.3	11.85	36.55	33.300000000000004
3	17.875	15.9	24.224999999999998	42.0
4	22.0	23.75	22.3	31.95
5	21.675	29.45	26.200000000000003	22.675
6	18.375	32.65	28.050000000000004	20.925
7	14.299999999999999	23.025000000000002	41.449999999999996	21.224999999999998
8	16.7	22.775000000000002	33.050000000000004	27.474999999999998
9	16.525000000000002	24.025	35.025	24.425
10	18.65	35.725	25.3	20.325
11	21.475	26.650000000000002	23.150000000000002	28.725
12	20.5	22.900000000000002	28.025	28.575
13	17.224999999999998	23.35	30.349999999999998	29.075
14	18.3	25.55	29.4	26.75
15	20.05	25.174999999999997	29.15	25.624999999999996
16	18.525	27.275	29.45	24.75
17	18.875	25.4	29.375	26.35
18	19.825	25.074999999999996	30.175	24.925
19	20.225	25.55	27.474999999999998	26.75
20	19.725	27.575	28.775000000000002	23.925
21	20.825	28.325	26.924999999999997	23.925
22	19.475	27.800000000000004	28.725	24.0
23	19.675	27.150000000000002	27.175	26.0
24	18.375	28.799999999999997	27.075	25.75
25	17.775	27.6	28.050000000000004	26.575
26	17.5	29.799999999999997	27.575	25.124999999999996
27	19.15	28.449999999999996	26.35	26.05
28	18.5	27.55	27.925	26.025
29	18.45	27.05	29.675	24.825
30	18.8	28.15	28.175	24.875
31	18.35	26.974999999999998	29.75	24.925
32	19.5	27.700000000000003	27.425	25.374999999999996
33	19.825	27.35	28.725	24.099999999999998
34	17.825	27.950000000000003	29.525000000000002	24.7
35	19.625	25.575	29.5	25.3
36	19.0	25.874999999999996	26.200000000000003	28.925
37	17.575	28.349999999999998	26.75	27.325
38	18.85	28.575	27.05	25.525
39	20.325	26.25	27.224999999999998	26.200000000000003
40	17.724999999999998	27.775	28.9	25.6
41	20.75	25.674999999999997	28.15	25.424999999999997
42	23.200000000000003	26.55	26.35	23.9
43	20.625	27.35	27.85	24.175
44	22.725	26.3	26.450000000000003	24.525
45	22.6	26.85	25.85	24.7
46	22.675	25.900000000000002	25.174999999999997	26.25
47	21.15	28.025	27.200000000000003	23.625
48	20.825	27.05	26.0	26.125
49	18.875	27.525	27.900000000000002	25.7
50	18.575	28.975	27.900000000000002	24.55
51	20.724999999999998	28.95	27.35	22.975
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	1.5
10	2.0
11	2.0
12	2.0
13	1.5
14	1.0
15	1.0
16	1.0
17	1.0
18	1.0
19	2.5
20	4.0
21	4.5
22	5.0
23	8.0
24	11.0
25	11.5
26	13.0
27	14.0
28	26.0
29	38.0
30	55.5
31	73.0
32	95.5
33	118.0
34	137.5
35	157.0
36	180.5
37	204.0
38	253.5
39	303.0
40	323.5
41	344.0
42	333.0
43	322.0
44	344.5
45	367.0
46	367.5
47	368.0
48	387.0
49	406.0
50	368.5
51	331.0
52	307.0
53	283.0
54	240.5
55	198.0
56	189.0
57	180.0
58	145.0
59	110.0
60	84.5
61	59.0
62	52.0
63	45.0
64	30.0
65	15.0
66	16.5
67	18.0
68	12.5
69	7.0
70	3.5
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35000000000000003
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.050000000000004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.29888027562446	42.55
2	13.608957795004306	15.8
3	4.565030146425495	7.95
4	2.885443583118002	6.7
5	1.6795865633074936	4.875
6	1.2489233419465977	4.35
7	0.6459948320413437	2.625
8	0.516795865633075	2.4
9	0.17226528854435832	0.8999999999999999
>10	1.3781223083548666	11.85
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAAT	30	0.75	No Hit
CGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCA	23	0.575	No Hit
CGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAAATTCTCCGCTCTGA	23	0.575	No Hit
CCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCCGAA	21	0.525	No Hit
ACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAAATTCTCCGCTCTG	21	0.525	No Hit
CTCGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAACG	20	0.5	No Hit
CTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATAAC	19	0.475	No Hit
CTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCT	18	0.44999999999999996	No Hit
CCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGT	17	0.42500000000000004	No Hit
CGCTTGACTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGGCACCTC	17	0.42500000000000004	No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCA	16	0.4	No Hit
CTCCAATTGTTCCTCGTTAAGGTATTTACATTGTACTCATTCCAATTACAA	15	0.375	No Hit
GTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAG	15	0.375	No Hit
CCTGTGGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAAAG	14	0.35000000000000003	No Hit
CTGTGGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAAAGG	14	0.35000000000000003	No Hit
CTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAGTTT	13	0.325	No Hit
CCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGGAAC	13	0.325	No Hit
CCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAA	13	0.325	No Hit
CCCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAA	13	0.325	No Hit
CCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTG	12	0.3	No Hit
CCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAAA	12	0.3	No Hit
CCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTC	12	0.3	No Hit
CACCAACTAAGAACGGCCATGCACCACCACCCACAAAATCAAGAAAGAGCT	11	0.27499999999999997	No Hit
CCGCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTGACATTCAGAGCA	11	0.27499999999999997	No Hit
ATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGC	11	0.27499999999999997	No Hit
CCAACAGCTATGCTCTTACTCAAATCCATCCGAAGACATCAGGATCGGTCG	10	0.25	No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTG	10	0.25	No Hit
GTAACATTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACC	10	0.25	No Hit
CTCGTACTAAGTTCAATTACTATTGCGGTAACATTCATCAGTAGGGTAAAA	10	0.25	No Hit
GCGGTAACATTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAA	10	0.25	No Hit
CACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGCTT	10	0.25	No Hit
CGCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGA	10	0.25	No Hit
CTCCTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGA	9	0.22499999999999998	No Hit
CCCACTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAG	9	0.22499999999999998	No Hit
CCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTAC	9	0.22499999999999998	No Hit
AAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCC	9	0.22499999999999998	No Hit
CCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAATT	8	0.2	No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	8	0.2	No Hit
GTACTTTTTCACTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTAC	8	0.2	No Hit
CTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCCACACGGG	8	0.2	No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTT	8	0.2	No Hit
CCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTA	8	0.2	No Hit
GTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACA	8	0.2	No Hit
CCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAATG	8	0.2	No Hit
CGGTAACATTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAA	8	0.2	No Hit
GCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTTG	8	0.2	No Hit
GCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAA	8	0.2	No Hit
CCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAGCCT	8	0.2	No Hit
CTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTCGCAG	7	0.17500000000000002	No Hit
GTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAACGGCC	7	0.17500000000000002	No Hit
CCCAGCACGACGGAGTTTCACAAGATTACCAAGACCTCTCGGCCAAGGTTA	7	0.17500000000000002	No Hit
CTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCCTTAGGACATC	7	0.17500000000000002	No Hit
GTGCCGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAG	7	0.17500000000000002	No Hit
CCTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAGTT	7	0.17500000000000002	No Hit
TGACAATTGAATACTGATGCCCCCGACCGTCCCTATTAATCATTACGATGG	7	0.17500000000000002	No Hit
CCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTT	7	0.17500000000000002	No Hit
GCTACTACCACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGT	7	0.17500000000000002	No Hit
CTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTTC	7	0.17500000000000002	No Hit
CTCCGCTTCATTGAATAAGTAAAGAAACTATAAAGGTAGTGGTATTTCACT	7	0.17500000000000002	No Hit
CCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTTCGCCAAG	7	0.17500000000000002	No Hit
CTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTTGAGCTT	7	0.17500000000000002	No Hit
GCCCAGTGAAATGCGAGATTCCCCTACCCACAAGGAGCAGAGGGCACAAAA	7	0.17500000000000002	No Hit
CTGCTATCCTGAGGGAAACTTCGGCAGGAACCAGCTACTAGATGGTTCGAT	7	0.17500000000000002	No Hit
CCTAACTTTCGTTCTTGATTAATGAAAACGTCCTTGGCAAATGCTTTCGCA	6	0.15	No Hit
CCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATAA	6	0.15	No Hit
CTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCT	6	0.15	No Hit
GTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTA	6	0.15	No Hit
CCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTG	6	0.15	No Hit
CTCGTTAAGGTATTTACATTGTACTCATTCCAATTACAAGACCCGAATGGG	6	0.15	No Hit
CTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTGACT	6	0.15	No Hit
CCGACATCGAAGAATCAAAAAGCAATGTCGCTATGTACGCTTGACTGCCAC	6	0.15	No Hit
GCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGA	6	0.15	No Hit
GTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAGGTGCTGG	6	0.15	No Hit
CCCAAAGTTGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATT	6	0.15	No Hit
ACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTA	6	0.15	No Hit
CCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAATA	6	0.15	No Hit
CCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAATAG	6	0.15	No Hit
CCTCAGTCCCAGCTGGCAGTATTCCCACAGGCTATAATACTTACCGAGGCA	6	0.15	No Hit
CCTCACTACCCGACCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTA	6	0.15	No Hit
CACCATCTTTCGGGTCCCAACAGCTATGCTCTTACTCAAATCCATCCGAAG	6	0.15	No Hit
CTCTAATCATTCGCTTTACCTCATAAAACTGATACGAGCTTCTGCTATCCT	6	0.15	No Hit
CCCTGTGGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAAA	6	0.15	No Hit
CAGTGAAATGCGAGATTCCCCTACCCACAAGGAGCAGAGGGCACAAAACAC	6	0.15	No Hit
CTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTTCGCCAAGA	6	0.15	No Hit
GTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAATAGAACCAAA	6	0.15	No Hit
GGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT	6	0.15	No Hit
CCACTATTTAGTAGGTTAAGGTCTCGTTCGTTATCGCAATTAAGCAGACAA	6	0.15	No Hit
CTCCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAAGA	6	0.15	No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTG	6	0.15	No Hit
GGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAAAGGATCG	6	0.15	No Hit
CACCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTT	6	0.15	No Hit
GCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCCG	6	0.15	No Hit
CCTTGGCAAATGCTTTCGCAGTAGTTAGTCTTCAATAAATCCAAGAATTTC	5	0.125	No Hit
CTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTATCG	5	0.125	No Hit
CCAGATTTCAAATTTGAGCTTTTGCCGCTTCACTCGCCGTTACTAAGGCAA	5	0.125	No Hit
CCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCC	5	0.125	No Hit
CTCCACGCCTGCCTACTCGTCAGGGCATCATATCAACCCTGACGGTAGAGT	5	0.125	No Hit
CTTGGCAAATGCTTTCGCAGTAGTTAGTCTTCAATAAATCCAAGAATTTCA	5	0.125	No Hit
CCTGCCACCAAAACTGATGCTGGCCCAGTGAAATGCGAGATTCCCCTACCC	5	0.125	No Hit
GTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAAAGGATCGA	5	0.125	No Hit
GGGAAACTTCGGCAGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCC	5	0.125	No Hit
CTCTTACTCAAATCCATCCGAAGACATCAGGATCGGTCGATTGTGCACCTC	5	0.125	No Hit
CCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCG	5	0.125	No Hit
CTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGGAACCTTTCCC	5	0.125	No Hit
CCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTATTCTAT	5	0.125	No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAAATTCGACGATCGATTTGCACG	5	0.125	No Hit
CTGAGGGAAACTTCGGCAGGAACCAGCTACTAGATGGTTCGATTAGTCTTT	5	0.125	No Hit
CCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATA	5	0.125	No Hit
GCCACACTTTCATGGTTTGTATTCACACTGAAAATCAAAATCAAGGGGGCT	5	0.125	No Hit
GTCTGATGAGCGTGTATTCCGGCACCTTAACTCTACGTTCGGTTCATCCCG	5	0.125	No Hit
GTCACTACCTCCCTGAATTAGGATTGGGTAATTTGCGCGCCTGCTGCCTTC	5	0.125	No Hit
TGCCGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGC	5	0.125	No Hit
CCGCTTCATTGAATAAGTAAAGAAACTATAAAGGTAGTGGTATTTCACTGG	5	0.125	No Hit
GTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTGATAGGGCAGA	5	0.125	No Hit
CTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACC	5	0.125	No Hit
CTAGAAACCAACAAAATAGAACCAAACGTCCTATTCTATTATTCCATGCTA	5	0.125	No Hit
CCCTTCTCTAAGCAGATCCTGAGGCCTCACTAAGCCATTCAATCGGTACTA	5	0.125	No Hit
CTGAATTAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGT	5	0.125	No Hit
CGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCAGAACCCA	5	0.125	No Hit
CGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAACGGC	5	0.125	No Hit
CCGGAATCGAACCCTTATTCCCCGTTACCCGTTGAAACCATGGTAGGCCAC	5	0.125	No Hit
CGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCA	5	0.125	No Hit
CAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAG	5	0.125	No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCTATTTTGCCGACTTCCC	5	0.125	No Hit
CCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAATGAC	5	0.125	No Hit
CTCTAAGAAGTGGATAACCAGCAAATGCTAGCACCACTATTTAGTAGGTTA	5	0.125	No Hit
CCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAAT	5	0.125	No Hit
CTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCAT	5	0.125	No Hit
TATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAG	5	0.125	No Hit
CTTATTCGTATCCATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTA	5	0.125	No Hit
CTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAAATTCTCC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.025	0.0	0.0	0.0	0.0
25	0.025	0.0	0.0	0.0	0.0
26	0.025	0.0	0.0	0.0	0.0
27	0.025	0.0	0.0	0.0	0.0
28	0.025	0.0	0.0	0.0	0.0
29	0.025	0.0	0.0	0.0	0.0
30	0.025	0.0	0.0	0.0	0.0
31	0.025	0.0	0.0	0.0	0.0
32	0.025	0.0	0.0	0.0	0.0
33	0.025	0.0	0.0	0.0	0.0
34	0.025	0.0	0.0	0.0	0.0
35	0.025	0.0	0.0	0.0	0.0
36	0.025	0.0	0.0	0.0	0.0
37	0.025	0.0	0.0	0.0	0.0
38	0.025	0.0	0.0	0.0	0.0
39	0.025	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR27822276 read2 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR27822276_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.33325	37.0	37.0	37.0	37.0	37.0
2	36.1995	37.0	37.0	37.0	37.0	37.0
3	36.214	37.0	37.0	37.0	37.0	37.0
4	36.142	37.0	37.0	37.0	37.0	37.0
5	36.37075	37.0	37.0	37.0	37.0	37.0
6	36.2215	37.0	37.0	37.0	37.0	37.0
7	36.3285	37.0	37.0	37.0	37.0	37.0
8	36.29225	37.0	37.0	37.0	37.0	37.0
9	36.291	37.0	37.0	37.0	37.0	37.0
10	36.34475	37.0	37.0	37.0	37.0	37.0
11	36.427	37.0	37.0	37.0	37.0	37.0
12	36.37125	37.0	37.0	37.0	37.0	37.0
13	36.31925	37.0	37.0	37.0	37.0	37.0
14	36.2535	37.0	37.0	37.0	37.0	37.0
15	36.32025	37.0	37.0	37.0	37.0	37.0
16	36.3355	37.0	37.0	37.0	37.0	37.0
17	36.2735	37.0	37.0	37.0	37.0	37.0
18	36.27575	37.0	37.0	37.0	37.0	37.0
19	36.298	37.0	37.0	37.0	37.0	37.0
20	36.23675	37.0	37.0	37.0	37.0	37.0
21	36.2385	37.0	37.0	37.0	37.0	37.0
22	36.2855	37.0	37.0	37.0	37.0	37.0
23	36.2445	37.0	37.0	37.0	37.0	37.0
24	36.2585	37.0	37.0	37.0	37.0	37.0
25	36.358	37.0	37.0	37.0	37.0	37.0
26	36.2695	37.0	37.0	37.0	37.0	37.0
27	36.163	37.0	37.0	37.0	37.0	37.0
28	36.3105	37.0	37.0	37.0	37.0	37.0
29	36.245	37.0	37.0	37.0	37.0	37.0
30	36.29375	37.0	37.0	37.0	37.0	37.0
31	36.27625	37.0	37.0	37.0	37.0	37.0
32	36.165	37.0	37.0	37.0	37.0	37.0
33	36.20275	37.0	37.0	37.0	37.0	37.0
34	36.18975	37.0	37.0	37.0	37.0	37.0
35	36.2175	37.0	37.0	37.0	37.0	37.0
36	36.26275	37.0	37.0	37.0	37.0	37.0
37	36.1695	37.0	37.0	37.0	37.0	37.0
38	36.06325	37.0	37.0	37.0	37.0	37.0
39	36.132	37.0	37.0	37.0	37.0	37.0
40	36.12175	37.0	37.0	37.0	37.0	37.0
41	36.18675	37.0	37.0	37.0	37.0	37.0
42	36.13075	37.0	37.0	37.0	37.0	37.0
43	36.0465	37.0	37.0	37.0	37.0	37.0
44	36.21775	37.0	37.0	37.0	37.0	37.0
45	36.13325	37.0	37.0	37.0	37.0	37.0
46	36.24775	37.0	37.0	37.0	37.0	37.0
47	36.164	37.0	37.0	37.0	37.0	37.0
48	36.09475	37.0	37.0	37.0	37.0	37.0
49	36.03225	37.0	37.0	37.0	37.0	37.0
50	36.05325	37.0	37.0	37.0	37.0	37.0
51	36.07175	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	1.0
17	0.0
18	2.0
19	1.0
20	1.0
21	0.0
22	2.0
23	2.0
24	4.0
25	4.0
26	1.0
27	6.0
28	8.0
29	11.0
30	14.0
31	21.0
32	34.0
33	56.0
34	107.0
35	487.0
36	2152.0
37	1084.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.632158039509875	19.179794948737182	13.003250812703177	39.184796199049764
2	25.75	24.099999999999998	35.099999999999994	15.049999999999999
3	21.55	25.825	30.25	22.375
4	23.5	36.275	22.0	18.224999999999998
5	27.906976744186046	35.95898974743686	20.305076269067268	15.828957239309826
6	24.45	36.55	21.775	17.224999999999998
7	22.425	21.575	37.925	18.075
8	24.18104526131533	23.58089522380595	30.132533133283324	22.1055263815954
9	25.074999999999996	24.85	30.475	19.6
10	22.9057264316079	35.483870967741936	23.93098274568642	17.67941985496374
11	29.425	26.6	21.45	22.525000000000002
12	30.332583145786447	22.330582645661416	24.60615153788447	22.73068267066767
13	23.80595148787197	27.181795448862218	26.03150787696924	22.980745186296573
14	24.575	30.025000000000002	27.825	17.575
15	24.081020255063766	31.08277069267317	26.30657664416104	18.529632408102024
16	26.6	26.674999999999997	27.725	19.0
17	26.05	31.025000000000002	24.675	18.25
18	27.831957989497376	26.906726681670417	25.28132033008252	19.979994998749685
19	24.45	29.049999999999997	27.075	19.425
20	24.456114028507127	32.75818954738685	24.981245311327832	17.804451112778192
21	24.4	29.625	28.050000000000004	17.925
22	25.2	26.900000000000002	27.725	20.175
23	26.125	28.525	28.050000000000004	17.299999999999997
24	25.525	29.875	25.1	19.5
25	25.275	28.325	26.8	19.6
26	25.374999999999996	30.925000000000004	25.374999999999996	18.325
27	25.974999999999998	30.925000000000004	24.9	18.2
28	25.374999999999996	28.875	26.0	19.75
29	26.55	29.2	26.25	18.0
30	23.85596399099775	28.732183045761438	26.30657664416104	21.10527631907977
31	25.481370342585645	28.457114278569644	27.581895473868467	18.479619904976243
32	23.799999999999997	29.75	26.0	20.45
33	24.656164041010253	28.107026756689173	26.18154538634659	21.05526381595399
34	22.55563890972743	30.882720680170046	25.93148287071768	20.630157539384847
35	23.9	31.025000000000002	26.375	18.7
36	24.88122030507627	29.232308077019255	28.532133033258315	17.35433858464616
37	26.424999999999997	27.725	26.075	19.775000000000002
38	26.531632908227053	29.507376844211052	25.95648912228057	18.00450112528132
39	28.475	29.599999999999998	23.025000000000002	18.9
40	23.13078269567392	29.707426856714182	26.106526631657918	21.05526381595399
41	26.006501625406354	28.982245561390346	26.38159539884971	18.629657414353588
42	24.956239059764943	28.80720180045011	23.78094523630908	22.455613903475868
43	25.874999999999996	29.075	26.5	18.55
44	24.131032758189548	28.68217054263566	26.18154538634659	21.005251312828207
45	23.680920230057513	30.132533133283324	27.7569392348087	18.42960740185046
46	26.831707926981746	28.132033008252062	24.956239059764943	20.080020005001252
47	24.8	28.4	26.6	20.200000000000003
48	25.55638909727432	28.75718929732433	25.98149537384346	19.70492623155789
49	23.23080770192548	28.75718929732433	29.35733933483371	18.65466366591648
50	25.656414103525883	27.731932983245812	27.306826706676667	19.30482620655164
51	26.18154538634659	28.207051762940733	28.307076769192296	17.30432608152038
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	1.0
16	1.0
17	2.5
18	4.0
19	3.0
20	2.0
21	7.0
22	12.0
23	12.0
24	12.0
25	20.5
26	28.5
27	28.0
28	45.0
29	62.0
30	88.5
31	115.0
32	101.0
33	87.0
34	128.0
35	169.0
36	216.0
37	263.0
38	252.0
39	241.0
40	283.5
41	326.0
42	396.5
43	467.0
44	463.0
45	459.0
46	420.5
47	382.0
48	363.0
49	344.0
50	315.5
51	287.0
52	251.5
53	216.0
54	180.0
55	144.0
56	150.0
57	156.0
58	112.0
59	68.0
60	59.5
61	51.0
62	41.5
63	32.0
64	27.5
65	23.0
66	15.5
67	8.0
68	5.0
69	2.0
70	1.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.5
78	1.0
79	1.0
80	1.0
81	0.5
82	0.0
83	0.0
84	0.0
85	0.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	1.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025
2	0.0
3	0.0
4	0.0
5	0.025
6	0.0
7	0.0
8	0.025
9	0.0
10	0.025
11	0.0
12	0.025
13	0.025
14	0.0
15	0.025
16	0.0
17	0.0
18	0.025
19	0.0
20	0.025
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.025
31	0.025
32	0.0
33	0.025
34	0.025
35	0.0
36	0.025
37	0.0
38	0.025
39	0.0
40	0.025
41	0.025
42	0.025
43	0.0
44	0.025
45	0.025
46	0.025
47	0.0
48	0.025
49	0.025
50	0.025
51	0.025
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.324999999999996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.5491512041058	46.575
2	14.56770627714173	18.45
3	5.48756415317805	10.424999999999999
4	3.158310303987367	8.0
5	1.342281879194631	4.25
6	0.7106198183971575	2.7
7	0.2763521515988946	1.225
8	0.07895775759968417	0.4
9	0.11843663639952626	0.675
>10	0.7106198183971575	7.3
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTG	36	0.8999999999999999	No Hit
CAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA	32	0.8	No Hit
CCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAA	23	0.575	No Hit
CTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAAA	20	0.5	No Hit
CTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAGT	16	0.4	No Hit
GGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATG	16	0.4	No Hit
CGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATT	16	0.4	No Hit
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGA	14	0.35000000000000003	No Hit
CACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGAT	14	0.35000000000000003	No Hit
ACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATT	13	0.325	No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT	13	0.325	No Hit
CTTGGATTTATTGAAGACTAACTACTGCGAAAGCATTTGCCAAGGACGTTT	13	0.325	No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGT	13	0.325	No Hit
CAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCTA	12	0.3	No Hit
CTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTTAG	11	0.27499999999999997	No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG	10	0.25	No Hit
GGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAA	10	0.25	No Hit
GTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAAC	10	0.25	No Hit
GTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGA	9	0.22499999999999998	No Hit
ACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGG	9	0.22499999999999998	No Hit
CGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTGC	9	0.22499999999999998	No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGT	8	0.2	No Hit
GTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGG	8	0.2	No Hit
GTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTTAACCTACTAAAT	7	0.17500000000000002	No Hit
CAAAGATTAAGCCATGCATGTCTAAGTATAAGCAATTTATACAGTGAAACT	7	0.17500000000000002	No Hit
CAGTATTCAATTGTCAGAGGTGAAATTCTTGGATTTATTGAAGACTAACTA	7	0.17500000000000002	No Hit
ATTGGCTCTAAGGGTCGGGTAGTGAGGGCCTTGGTCAGACGCAGCGGGCGT	7	0.17500000000000002	No Hit
GCCATGCATGTCTAAGTATAAGCAATTTATACAGTGAAACTGCGAATGGCT	7	0.17500000000000002	No Hit
CTTAGACGTTCTGGGCCGCACGCGCGCTACACTGACGGAGCCAGCGAGTCT	7	0.17500000000000002	No Hit
GTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGT	7	0.17500000000000002	No Hit
CTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAGCG	6	0.15	No Hit
AGCTACCATCCGCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCCATGC	6	0.15	No Hit
CCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAGAAGGGGGCAA	6	0.15	No Hit
GAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGCATTTGATCAGACATGGT	6	0.15	No Hit
GTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGT	6	0.15	No Hit
GATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAGAAGGGGGCAACT	6	0.15	No Hit
CTGATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAATA	6	0.15	No Hit
AACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCT	6	0.15	No Hit
CTTGAGTCCTTGTGGCTCTTGGCGAACCAGGACTTTTACTTTGAAAAAATT	6	0.15	No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTTTGACATTGTGAAGAGACATAGA	6	0.15	No Hit
CTTCGATGTCGGCTCTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTG	6	0.15	No Hit
ATCGGTTTCAAGCCGATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCC	6	0.15	No Hit
AGTGAACGTAGGTTGGGGCCTCGCAAGAGGTGCACAATCGACCGATCCTGA	6	0.15	No Hit
CAAAAGGGTAAAAGCCCCCTTGATTTTGATTTTCAGTGTGAATACAAACCA	6	0.15	No Hit
CTTTAAATATGTAAGAAGTCCTTGTTACTTAATTGAACGTGGACATTTGAA	6	0.15	No Hit
GTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGCGGGAGTAACTAT	6	0.15	No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACG	6	0.15	No Hit
GTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAA	6	0.15	No Hit
AAGAAATTCAACCAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTT	5	0.125	No Hit
GTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGG	5	0.125	No Hit
CTTTTCTTCTTAACAGCTTATCACCCCGGAATTGGTTTATCCGGAGATGGG	5	0.125	No Hit
ATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAAC	5	0.125	No Hit
CCTTAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTAC	5	0.125	No Hit
TGAAAATCCACAGGAAGGAATAGTTTTCATGCCAGGTCGTACTGATAACCG	5	0.125	No Hit
GCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTGTTGAG	5	0.125	No Hit
GATTAAGCCATGCATGTCTAAGTATAAGCAATTTATACAGTGAAACTGCGA	5	0.125	No Hit
ATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACA	5	0.125	No Hit
AACAACTCACCGGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGC	5	0.125	No Hit
CAGGACTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTATTGCTC	5	0.125	No Hit
CGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAA	5	0.125	No Hit
CTTAACCATAAACTATGCCGACTAGGGATCGGGTGGTGTTTTTTTAATGAC	5	0.125	No Hit
CCTAATTCAGGGAGGTAGTGACAATAAATAACGATACAGGGCCCATTCGGG	5	0.125	No Hit
CCGGAGATGGGGTCTTATGGCTGGAAGAGGCCAGCACCTTTGCTGGCTCCG	5	0.125	No Hit
CCACTTCTTAGAGGGACTATCGGTTTCAAGCCGATGGAAGTTTGAGGCAAT	5	0.125	No Hit
GGACATTTGAATGAAGAGCTTTTAGTGGGCCATTTTTGGTAAGCAGAACTG	5	0.125	No Hit
GTAAGAAGTCCTTGTTACTTAATTGAACGTGGACATTTGAATGAAGAGCTT	5	0.125	No Hit
AGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACC	5	0.125	No Hit
CAACTCACCGGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGT	5	0.125	No Hit
CTTCGGGATAAGGATTGGCTCTAAGGGTCGGGTAGTGAGGGCCTTGGTCAG	5	0.125	No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGAT	5	0.125	No Hit
GCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGAT	5	0.125	No Hit
CGAAAGGGAATCCGGTTAAGATTCCGGAACCTGGATATGGATTCTTCACGG	5	0.125	No Hit
GGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAAAAGCCCCCTTGATTTT	5	0.125	No Hit
GGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTA	5	0.125	No Hit
GTTCATTCGGATAATTGGTTTTTGCGGCTGTCTGATCAGGCATTGCCGCGA	5	0.125	No Hit
ACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTT	5	0.125	No Hit
GGAGGAGTTATCTTTTCTTCTTAACAGCTTATCACCCCGGAATTGGTTTAT	5	0.125	No Hit
GACAGATTGAGAGCTCTTTCTTGATTTTGTGGGTGGTGGTGCATGGCCGTT	5	0.125	No Hit
GATTAATAGGGACGGTCGGGGGCATCAGTATTCAATTGTCAGAGGTGAAAT	5	0.125	No Hit
CGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTA	5	0.125	No Hit
TAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACC	5	0.125	No Hit
GTCGGCTCTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.025	0.0	0.0	0.0	0.0
25	0.025	0.0	0.0	0.0	0.0
26	0.025	0.0	0.0	0.0	0.0
27	0.025	0.0	0.0	0.0	0.0
28	0.025	0.0	0.0	0.0	0.0
29	0.025	0.0	0.0	0.0	0.0
30	0.025	0.0	0.0	0.0	0.0
31	0.025	0.0	0.0	0.0	0.0
32	0.025	0.0	0.0	0.0	0.0
33	0.025	0.0	0.0	0.0	0.0
34	0.025	0.0	0.0	0.0	0.0
35	0.025	0.0	0.0	0.0	0.0
36	0.025	0.0	0.0	0.0	0.0
37	0.025	0.0	0.0	0.0	0.0
38	0.025	0.0	0.0	0.0	0.0
39	0.025	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 1218157 spots for SRR27822276.sra
Written 1218157 spots for SRR27822276.sra
Read 1218157 spots for SRR27822276.sra
Written 1218157 spots for SRR27822276.sra
Read 1218157 spots for SRR27822276.sra
Written 1218157 spots for SRR27822276.sra
Read 1218157 spots for SRR27822276.sra
Written 1218157 spots for SRR27822276.sra
Read 1218157 spots for SRR27822276.sra
Written 1218157 spots for SRR27822276.sra
Read 1218159 spots for SRR27822276.sra
Written 1218159 spots for SRR27822276.sra
Read 1218157 spots for SRR27822276.sra
Written 1218157 spots for SRR27822276.sra
Read 1218157 spots for SRR27822276.sra
Written 1218157 spots for SRR27822276.sra
SRR ids: ['SRR27822276.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_g2ptyx6a
SRR27822276.sra spots: 9745258
blocks: [[1, 1218157], [1218158, 2436314], [2436315, 3654471], [3654472, 4872628], [4872629, 6090785], [6090786, 7308942], [7308943, 8527099], [8527100, 9745258]]
SRR27822276 file size 1678316
SRR27822276 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR27822276 SRR27822276_1.fastq SRR27822276_2.fastq
Input file:	SRR27822276_1.fastq
Paired file:	SRR27822276_2.fastq
trimmed:	SRR27822276-trimmed-pair1.fastq, SRR27822276-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Thu May 14 08:09:24 2026 >> started

Thu May 14 08:09:41 2026 >> done (16.855s)
9745258 read pairs processed; of these:
     80 ( 0.00%) short read pairs filtered out after trimming by size control
   1810 ( 0.02%) empty read pairs filtered out after trimming by size control
9743368 (99.98%) read pairs available; of these:
   8521 ( 0.09%) trimmed read pairs available after processing
9734847 (99.91%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	      8	  0.00%
 19	     79	  0.00%
 20	     10	  0.00%
 21	      8	  0.00%
 22	      6	  0.00%
 23	     18	  0.00%
 24	      4	  0.00%
 25	     21	  0.00%
 26	      7	  0.00%
 27	     25	  0.00%
 28	     22	  0.00%
 29	     25	  0.00%
 30	     22	  0.00%
 31	     46	  0.00%
 32	     33	  0.00%
 33	     56	  0.00%
 34	     77	  0.00%
 35	     90	  0.00%
 36	    107	  0.00%
 37	    135	  0.00%
 38	    226	  0.00%
 39	    252	  0.00%
 40	    295	  0.00%
 41	    363	  0.00%
 42	    434	  0.00%
 43	    408	  0.00%
 44	    418	  0.00%
 45	    542	  0.01%
 46	    660	  0.01%
 47	    739	  0.01%
 48	    945	  0.01%
 49	   1128	  0.01%
 50	   1312	  0.01%
 51	9734847	 99.91%
9743368 reads passed initial QC


criterion=sequence-density
sequence-density=0.28
sequence-density-rank=1
fanout-score=2.38
fanout-score-rank=9
prefix-density=0.27
prefix-fanout=2.4
sequence=ACGCTTGACTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAAAGGATCGATAGGCCACACTTTCATGGTTTGTATTCACACTGAAAATCAAAATCAAGGGGGCTTTTACCCTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCCTTAGGACATCTGCGTTATCGTTT


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=8
fanout-score=22.83
fanout-score-rank=1
prefix-density=0.73
prefix-fanout=1.0
sequence=ACAAGACCCGACTGGGCCCTGTA


criterion=sequence-density
sequence-density=0.32
sequence-density-rank=1
fanout-score=2.58
fanout-score-rank=19
prefix-density=0.33
prefix-fanout=2.5
sequence=CATAGCGACATTGCTTTTTGATTCTTCGATGTCGGCTCTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGAATGTTACCGCAATAGTAATTGAACTTAGTACGAGAGGAACAGTTCATTCGGATAATTGGTTTTTGCGGCTGTCTGATCAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAATGTCATTTTGCGTGGGGATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTAG


criterion=fanout-score
sequence-density=0.03
sequence-density-rank=5
fanout-score=70.44
fanout-score-rank=1
prefix-density=2.17
prefix-fanout=1.0
sequence=GTAAAACCCATCCGCGTAATGAA
SRR27822276 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 14 08:10:10
                             Started mapping on |	May 14 08:10:10
                                    Finished on |	May 14 08:10:26
       Mapping speed, Million of reads per hour |	2192.26

                          Number of input reads |	9743368
                      Average input read length |	101
                                    UNIQUE READS:
                   Uniquely mapped reads number |	2421374
                        Uniquely mapped reads % |	24.85%
                          Average mapped length |	101.83
                       Number of splices: Total |	44439
            Number of splices: Annotated (sjdb) |	38651
                       Number of splices: GT/AG |	41755
                       Number of splices: GC/AG |	121
                       Number of splices: AT/AC |	63
               Number of splices: Non-canonical |	2500
                      Mismatch rate per base, % |	0.08%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.30
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.08
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	7198944
             % of reads mapped to multiple loci |	73.89%
        Number of reads mapped to too many loci |	62127
             % of reads mapped to too many loci |	0.64%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.62%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	123050	123050	123050
N_multimapping	7198944	7198944	7198944
N_noFeature	97826	2252207	153517
N_ambiguous	135988	1017	24942
UnstrandedReadsAssigned:2187560 PositiveStrandReadsAssigned:168150 NegativeStrandReadsAssigned:2242915
Dataset is classified negative stranded
MeadianReadLen=51 20thPercentileLength=51 echo kmer=47
SRR27822276 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 6,713
[index] number of k-mers: 8,283,195
[index] number of equivalence classes: 8,710
[quant] running in paired-end mode
[quant] will process pair 1: SRR27822276-trimmed-pair1.fastq
                             SRR27822276-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 9,743,368 reads, 2,584,573 reads pseudoaligned
[quant] estimated average fragment length: 200.779
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 635 rounds

  6714 SRR27822276.ke.tsv
  7126 SRR27822276.se.tsv
 13840 total
==> SRR27822276.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
YHR055C	186	58.4102	170.5	306.051
YPR161C	1974	1774.22	248	14.6556
YOL138C	4026	3826.22	273	7.48085
YDR395W	2835	2635.22	732	29.1241
YGR129W	648	449.011	90	21.0157
YPR165W	630	431.27	545	132.497
YPR098C	486	292.455	91	32.6243
YPL015C	1074	874.221	208	24.946
YCL050C	966	766.221	590	80.734

==> SRR27822276.se.tsv <==
YDL248W	32
YDL247W-A	0
YDL247W	2
YDL246C	0
YDL245C	0
YDL244W	5
YDL243C	60
YDL242W	0
YDL241W	20
YDL240C-A	0
SRR27822276 completed mapping pipeline successfully
