Starting /dee2/code/volunteer_pipeline.sh SRR27822271
    current disk space = 192084280
    free memory = 129597208 
SRR27822271 SRAfilesize
98980faa33c768244439fed2c174bd2e  SRR27822271.sra
SRR27822271.sra file validated
SRR27822271 is paired end
SRR27822271 is conventional basespace
SRR27822271 read1 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR27822271_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.44675	37.0	37.0	37.0	37.0	37.0
2	36.518	37.0	37.0	37.0	37.0	37.0
3	36.685	37.0	37.0	37.0	37.0	37.0
4	36.6995	37.0	37.0	37.0	37.0	37.0
5	36.613	37.0	37.0	37.0	37.0	37.0
6	36.554	37.0	37.0	37.0	37.0	37.0
7	36.593	37.0	37.0	37.0	37.0	37.0
8	36.614	37.0	37.0	37.0	37.0	37.0
9	36.633	37.0	37.0	37.0	37.0	37.0
10	36.6755	37.0	37.0	37.0	37.0	37.0
11	36.6295	37.0	37.0	37.0	37.0	37.0
12	36.6765	37.0	37.0	37.0	37.0	37.0
13	36.621	37.0	37.0	37.0	37.0	37.0
14	36.586	37.0	37.0	37.0	37.0	37.0
15	36.5745	37.0	37.0	37.0	37.0	37.0
16	36.569	37.0	37.0	37.0	37.0	37.0
17	36.6035	37.0	37.0	37.0	37.0	37.0
18	36.5645	37.0	37.0	37.0	37.0	37.0
19	36.5885	37.0	37.0	37.0	37.0	37.0
20	36.5765	37.0	37.0	37.0	37.0	37.0
21	36.6075	37.0	37.0	37.0	37.0	37.0
22	36.6345	37.0	37.0	37.0	37.0	37.0
23	36.557	37.0	37.0	37.0	37.0	37.0
24	36.491	37.0	37.0	37.0	37.0	37.0
25	36.531	37.0	37.0	37.0	37.0	37.0
26	36.584	37.0	37.0	37.0	37.0	37.0
27	36.541	37.0	37.0	37.0	37.0	37.0
28	36.5855	37.0	37.0	37.0	37.0	37.0
29	36.61	37.0	37.0	37.0	37.0	37.0
30	36.492	37.0	37.0	37.0	37.0	37.0
31	36.559	37.0	37.0	37.0	37.0	37.0
32	36.5815	37.0	37.0	37.0	37.0	37.0
33	36.5375	37.0	37.0	37.0	37.0	37.0
34	36.541	37.0	37.0	37.0	37.0	37.0
35	36.4865	37.0	37.0	37.0	37.0	37.0
36	36.356	37.0	37.0	37.0	37.0	37.0
37	36.4765	37.0	37.0	37.0	37.0	37.0
38	36.485	37.0	37.0	37.0	37.0	37.0
39	36.491	37.0	37.0	37.0	37.0	37.0
40	36.4105	37.0	37.0	37.0	37.0	37.0
41	36.494	37.0	37.0	37.0	37.0	37.0
42	36.4485	37.0	37.0	37.0	37.0	37.0
43	36.3245	37.0	37.0	37.0	37.0	37.0
44	36.493	37.0	37.0	37.0	37.0	37.0
45	36.4465	37.0	37.0	37.0	37.0	37.0
46	36.39	37.0	37.0	37.0	37.0	37.0
47	36.5195	37.0	37.0	37.0	37.0	37.0
48	36.5235	37.0	37.0	37.0	37.0	37.0
49	36.4465	37.0	37.0	37.0	37.0	37.0
50	36.4425	37.0	37.0	37.0	37.0	37.0
51	36.475	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
26	2.0
27	3.0
28	7.0
29	7.0
30	10.0
31	23.0
32	17.0
33	36.0
34	68.0
35	225.0
36	1873.0
37	1729.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.31135622963149	10.579092504387065	7.771371270995236	59.338179994986206
2	20.275000000000002	13.125	35.625	30.975
3	18.65	17.2	24.2	39.95
4	23.0	23.275000000000002	22.55	31.175000000000004
5	20.125	30.125	27.85	21.9
6	19.3	35.425000000000004	26.525	18.75
7	14.85	23.674999999999997	40.699999999999996	20.775
8	15.325	23.075000000000003	33.45	28.15
9	14.475	25.0	37.5	23.025000000000002
10	19.125	35.875	23.724999999999998	21.275
11	21.275	27.825	22.25	28.65
12	21.25	24.175	26.150000000000002	28.425
13	15.0	24.925	30.125	29.95
14	17.1	26.8	29.099999999999998	27.0
15	19.525000000000002	24.825	30.275000000000002	25.374999999999996
16	18.775	25.7	28.425	27.1
17	18.5	25.4	29.099999999999998	27.0
18	17.8	23.175	30.7	28.325
19	19.725	24.575	28.599999999999998	27.1
20	18.925	27.6	28.299999999999997	25.174999999999997
21	19.575	28.1	26.674999999999997	25.650000000000002
22	20.3	27.075	28.675	23.95
23	20.974999999999998	27.425	27.425	24.175
24	18.8	28.7	26.8	25.7
25	18.099999999999998	29.25	28.249999999999996	24.4
26	17.5	28.65	28.449999999999996	25.4
27	18.05	30.45	26.575	24.925
28	18.375	27.900000000000002	26.075	27.650000000000002
29	17.150000000000002	25.6	32.025	25.224999999999998
30	16.725	27.750000000000004	29.849999999999998	25.674999999999997
31	19.5	27.0	29.625	23.875
32	21.175	27.750000000000004	27.400000000000002	23.674999999999997
33	21.9	25.775	28.875	23.45
34	20.375	26.1	28.175	25.35
35	21.9	25.825	27.025	25.25
36	17.525	26.35	26.974999999999998	29.15
37	16.85	28.849999999999998	26.0	28.299999999999997
38	20.225	27.35	26.424999999999997	26.0
39	19.175	27.6	26.700000000000003	26.525
40	18.525	28.575	28.275	24.625
41	19.950000000000003	27.200000000000003	26.85	26.0
42	21.825	28.199999999999996	26.650000000000002	23.325000000000003
43	21.85	25.525	28.65	23.974999999999998
44	22.85	24.75	27.6	24.8
45	23.549999999999997	28.449999999999996	23.625	24.375
46	20.424999999999997	25.35	25.3	28.925
47	22.5	26.424999999999997	27.775	23.3
48	19.950000000000003	26.200000000000003	28.425	25.424999999999997
49	19.625	25.224999999999998	29.7	25.45
50	19.2	27.775	27.6	25.424999999999997
51	20.375	28.525	27.625	23.474999999999998
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	2.0
12	4.0
13	3.0
14	2.0
15	2.5
16	3.0
17	1.5
18	0.0
19	2.0
20	4.0
21	7.0
22	10.0
23	9.5
24	9.0
25	15.0
26	25.0
27	29.0
28	36.5
29	44.0
30	69.5
31	95.0
32	130.0
33	165.0
34	158.5
35	152.0
36	153.5
37	155.0
38	209.0
39	263.0
40	299.5
41	336.0
42	308.0
43	280.0
44	334.5
45	389.0
46	372.5
47	356.0
48	357.5
49	359.0
50	356.5
51	354.0
52	317.0
53	280.0
54	257.5
55	235.0
56	212.5
57	190.0
58	156.5
59	123.0
60	90.5
61	58.0
62	52.0
63	46.0
64	29.5
65	13.0
66	14.5
67	16.0
68	12.5
69	9.0
70	4.5
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.27499999999999997
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.8
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.70068027210884	45.1
2	10.926870748299319	12.85
3	4.549319727891157	8.025
4	2.423469387755102	5.7
5	1.488095238095238	4.375
6	1.0204081632653061	3.5999999999999996
7	0.8078231292517007	3.325
8	0.4251700680272109	2.0
9	0.3401360544217687	1.7999999999999998
>10	1.2755102040816326	11.875
>50	0.04251700680272109	1.35
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCA	54	1.35	No Hit
GCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAA	37	0.9249999999999999	No Hit
CTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCT	27	0.675	No Hit
ACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAAATTCTCCGCTCTG	26	0.65	No Hit
CGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAAATTCTCCGCTCTGA	25	0.625	No Hit
CCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAAT	25	0.625	No Hit
CCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGT	22	0.5499999999999999	No Hit
GTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAG	21	0.525	No Hit
CCTGTGGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAAAG	19	0.475	No Hit
CTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATAAC	18	0.44999999999999996	No Hit
GTACTTTTTCACTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTAC	17	0.42500000000000004	No Hit
AAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCC	17	0.42500000000000004	No Hit
CGCTTGACTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGGCACCTC	15	0.375	No Hit
CTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAGTTT	14	0.35000000000000003	No Hit
CCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATAA	14	0.35000000000000003	No Hit
ATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGC	14	0.35000000000000003	No Hit
CCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCCGAA	13	0.325	No Hit
CCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAA	13	0.325	No Hit
CCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCCGA	12	0.3	No Hit
CATAAAACTGATACGAGCTTCTGCTATCCTGAGGGAAACTTCGGCAGGAAC	11	0.27499999999999997	No Hit
CTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAAATTCTCCGCTCTGAGAT	11	0.27499999999999997	No Hit
CTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTCAAA	11	0.27499999999999997	No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	11	0.27499999999999997	No Hit
CGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCA	11	0.27499999999999997	No Hit
GTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTA	11	0.27499999999999997	No Hit
CTCGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAACG	10	0.25	No Hit
CCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAAA	10	0.25	No Hit
CTAGAATTACCACAGTTATACCATGTAGTAAAGGAACTATCAAATAAACGA	10	0.25	No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCA	10	0.25	No Hit
CTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTTCGCCAAGA	10	0.25	No Hit
CGCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGA	10	0.25	No Hit
CCAGATTTCAAATTTGAGCTTTTGCCGCTTCACTCGCCGTTACTAAGGCAA	9	0.22499999999999998	No Hit
CCCATCTCTTAGGATCGACTAACCCACGTCCAACTGCTGTTGACGTGGAAC	9	0.22499999999999998	No Hit
CTGTGGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAAAGG	9	0.22499999999999998	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCA	9	0.22499999999999998	No Hit
GTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTACA	9	0.22499999999999998	No Hit
GCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTTG	9	0.22499999999999998	No Hit
CGTACTTTTTCACTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTA	9	0.22499999999999998	No Hit
CCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAGCCT	9	0.22499999999999998	No Hit
CTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTATCG	8	0.2	No Hit
GTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAACGGCC	8	0.2	No Hit
CCCAAAGTTGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATT	8	0.2	No Hit
CAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACC	8	0.2	No Hit
CACGTACTTTTTCACTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTG	8	0.2	No Hit
CTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGC	8	0.2	No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTT	8	0.2	No Hit
CTCTATGTCTCTTCACAATGTCAAACTAGAGTCAAGCTCAACAGGGTCTTC	8	0.2	No Hit
CCGGAATCGAACCCTTATTCCCCGTTACCCGTTGAAACCATGGTAGGCCAC	8	0.2	No Hit
CGAGATTTTAAGCATGTATTAGCTCTAGAATTACCACAGTTATACCATGTA	8	0.2	No Hit
CCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTAAAAGCTCTTCAT	7	0.17500000000000002	No Hit
CTCCACGCCTGCCTACTCGTCAGGGCATCATATCAACCCTGACGGTAGAGT	7	0.17500000000000002	No Hit
CGAAGACATTGATTTTTTATCTAATAAATACATCTCTTCCAAAGGGTCGAG	7	0.17500000000000002	No Hit
GTAACATTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACC	7	0.17500000000000002	No Hit
CACCAACTAAGAACGGCCATGCACCACCACCCACAAAATCAAGAAAGAGCT	7	0.17500000000000002	No Hit
CCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTT	7	0.17500000000000002	No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGG	7	0.17500000000000002	No Hit
GCGGTAACATTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAA	7	0.17500000000000002	No Hit
CTCCAATTGTTCCTCGTTAAGGTATTTACATTGTACTCATTCCAATTACAA	7	0.17500000000000002	No Hit
GTCGCAGTCCTCAGTCCCAGCTGGCAGTATTCCCACAGGCTATAATACTTA	7	0.17500000000000002	No Hit
CGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTTCGCCA	7	0.17500000000000002	No Hit
CCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAATGAC	7	0.17500000000000002	No Hit
CACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGCTT	7	0.17500000000000002	No Hit
CTTTCGCCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCT	7	0.17500000000000002	No Hit
CCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTAC	7	0.17500000000000002	No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCG	7	0.17500000000000002	No Hit
CCCCAAAGTTGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGAT	7	0.17500000000000002	No Hit
CAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCCGAATG	7	0.17500000000000002	No Hit
GCGGCATATAACCATTATGCCAGCATCCTTGACTTACGTCGCAGTCCTCAG	7	0.17500000000000002	No Hit
CGCAGTCCTCAGTCCCAGCTGGCAGTATTCCCACAGGCTATAATACTTACC	6	0.15	No Hit
CCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCC	6	0.15	No Hit
CCCAGCACGACGGAGTTTCACAAGATTACCAAGACCTCTCGGCCAAGGTTA	6	0.15	No Hit
CCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTGA	6	0.15	No Hit
CGAGCTTCTGCTATCCTGAGGGAAACTTCGGCAGGAACCAGCTACTAGATG	6	0.15	No Hit
CTTCAGTCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCT	6	0.15	No Hit
CCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGGGAAT	6	0.15	No Hit
CAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCGCC	6	0.15	No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAAATTCGACGATCGATTTGCACG	6	0.15	No Hit
AGCATGTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAAC	6	0.15	No Hit
CCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACCGCACTCCTCG	6	0.15	No Hit
CCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTC	6	0.15	No Hit
CTGTTATTGCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGA	6	0.15	No Hit
CTAGAAACCAACAAAATAGAACCAAACGTCCTATTCTATTATTCCATGCTA	6	0.15	No Hit
CCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTA	6	0.15	No Hit
CACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCTGCGTTCTTCAT	6	0.15	No Hit
GTCCCAACAGCTATGCTCTTACTCAAATCCATCCGAAGACATCAGGATCGG	6	0.15	No Hit
GCCGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCC	6	0.15	No Hit
CTCGCTGGCTCCGTCAGTGTAGCGCGCGTGCGGCCCAGAACGTCTAAGGGC	6	0.15	No Hit
CCCGGAACCTCTAATCATTCGCTTTACCTCATAAAACTGATACGAGCTTCT	6	0.15	No Hit
CATCTATATTGTGTGGAGCAAAGAAATCACCGCGTTCTAGCATGGATTCTG	6	0.15	No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG	6	0.15	No Hit
CTTGTTACGACTTTTAGTTCCTCTAAATGACCAAGTTTGTCCAAATTCTCC	6	0.15	No Hit
GCCCCAGCCAAACTCCCCACCTGACAATGTCTTCAACCCGGATCAGCCCCG	6	0.15	No Hit
CCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGAATT	5	0.125	No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGCTATGCTCTTACTC	5	0.125	No Hit
CCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACGGTCTAGGCTTCG	5	0.125	No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCA	5	0.125	No Hit
GGGAAACTTCGGCAGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCC	5	0.125	No Hit
CCCCTATACCCAAATTCGACGATCGATTTGCACGTCAGAACCGCTACGAGC	5	0.125	No Hit
CCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTG	5	0.125	No Hit
CCGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTA	5	0.125	No Hit
GTGGATTTTCACGGGCCGTCACAAGCGCACCGGAGCCAGCAAAGGTGCTGG	5	0.125	No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTT	5	0.125	No Hit
TGACAATTGAATACTGATGCCCCCGACCGTCCCTATTAATCATTACGATGG	5	0.125	No Hit
CTTTTTCACTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTG	5	0.125	No Hit
CCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATA	5	0.125	No Hit
CTCATTTGAATATTTGCTACTACCACCAAGATCTGCACTAGAGGCCGTTCG	5	0.125	No Hit
CTCGTACTAAGTTCAATTACTATTGCGGTAACATTCATCAGTAGGGTAAAA	5	0.125	No Hit
CCGCGTTCTAGCATGGATTCTGACTTAGAGGCGTTCAGCCATAATCCAGCG	5	0.125	No Hit
CGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAG	5	0.125	No Hit
ATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCTACCTTAA	5	0.125	No Hit
CTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCAGAACCCAAAGACTTTG	5	0.125	No Hit
CCCAGGGCTCGCGCCGACGTCTCCACATTCAGTTACGTTACCGTGAAGAAT	5	0.125	No Hit
CGTTCGTTATCGCAATTAAGCAGACAAATCACTCCACCAACTAAGAACGGC	5	0.125	No Hit
GGGTGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTG	5	0.125	No Hit
CGGTAACATTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAA	5	0.125	No Hit
CTCTAAGAAGTGGATAACCAGCAAATGCTAGCACCACTATTTAGTAGGTTA	5	0.125	No Hit
CGCCTGCCTACTCGTCAGGGCATCATATCAACCCTGACGGTAGAGTATAGG	5	0.125	No Hit
GCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAAG	5	0.125	No Hit
CGGGCGGCATATAACCATTATGCCAGCATCCTTGACTTACGTCGCAGTCCT	5	0.125	No Hit
CTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTACCGAATTCTGCT	5	0.125	No Hit
CCTGTTATTGCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAG	5	0.125	No Hit
CTGACAATTGAATACTGATGCCCCCGACCGTCCCTATTAATCATTACGATG	5	0.125	No Hit
CACCTCTGACAATTGAATACTGATGCCCCCGACCGTCCCTATTAATCATTA	5	0.125	No Hit
GGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAAAGGATCG	5	0.125	No Hit
CACACTTTCATGGTTTGTATTCACACTGAAAATCAAAATCAAGGGGGCTTT	5	0.125	No Hit
AAAACTGATACGAGCTTCTGCTATCCTGAGGGAAACTTCGGCAGGAACCAG	5	0.125	No Hit
GTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCTGCCAAGCCCGTTCC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.025	0.0	0.0	0.0	0.0
7	0.025	0.0	0.0	0.0	0.0
8	0.025	0.0	0.0	0.0	0.0
9	0.025	0.0	0.0	0.0	0.0
10	0.025	0.0	0.0	0.0	0.0
11	0.025	0.0	0.0	0.0	0.0
12	0.025	0.0	0.0	0.0	0.0
13	0.025	0.0	0.0	0.0	0.0
14	0.025	0.0	0.0	0.0	0.0
15	0.025	0.0	0.0	0.0	0.0
16	0.025	0.0	0.0	0.0	0.0
17	0.025	0.0	0.0	0.0	0.0
18	0.025	0.0	0.0	0.0	0.0
19	0.025	0.0	0.0	0.0	0.0
20	0.025	0.0	0.0	0.0	0.0
21	0.025	0.0	0.0	0.0	0.0
22	0.025	0.0	0.0	0.0	0.0
23	0.025	0.0	0.0	0.0	0.0
24	0.025	0.0	0.0	0.0	0.0
25	0.025	0.0	0.0	0.0	0.0
26	0.025	0.0	0.0	0.0	0.0
27	0.025	0.0	0.0	0.0	0.0
28	0.025	0.0	0.0	0.0	0.0
29	0.025	0.0	0.0	0.0	0.0
30	0.025	0.0	0.0	0.0	0.0
31	0.025	0.0	0.0	0.0	0.0
32	0.025	0.0	0.0	0.0	0.0
33	0.025	0.0	0.0	0.0	0.0
34	0.025	0.0	0.0	0.0	0.0
35	0.025	0.0	0.0	0.0	0.0
36	0.025	0.0	0.0	0.0	0.0
37	0.025	0.0	0.0	0.0	0.0
38	0.025	0.0	0.0	0.0	0.0
39	0.025	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
SRR27822271 read2 length is 51 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR27822271_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.1225	37.0	37.0	37.0	37.0	37.0
2	36.1905	37.0	37.0	37.0	37.0	37.0
3	36.3025	37.0	37.0	37.0	37.0	37.0
4	36.0905	37.0	37.0	37.0	37.0	37.0
5	36.264	37.0	37.0	37.0	37.0	37.0
6	36.224	37.0	37.0	37.0	37.0	37.0
7	36.03	37.0	37.0	37.0	37.0	37.0
8	36.3	37.0	37.0	37.0	37.0	37.0
9	36.3885	37.0	37.0	37.0	37.0	37.0
10	36.3325	37.0	37.0	37.0	37.0	37.0
11	36.365	37.0	37.0	37.0	37.0	37.0
12	36.2505	37.0	37.0	37.0	37.0	37.0
13	36.4	37.0	37.0	37.0	37.0	37.0
14	36.3435	37.0	37.0	37.0	37.0	37.0
15	36.308	37.0	37.0	37.0	37.0	37.0
16	36.3205	37.0	37.0	37.0	37.0	37.0
17	36.264	37.0	37.0	37.0	37.0	37.0
18	36.1445	37.0	37.0	37.0	37.0	37.0
19	36.2935	37.0	37.0	37.0	37.0	37.0
20	36.2845	37.0	37.0	37.0	37.0	37.0
21	36.248	37.0	37.0	37.0	37.0	37.0
22	36.2535	37.0	37.0	37.0	37.0	37.0
23	36.277	37.0	37.0	37.0	37.0	37.0
24	36.2665	37.0	37.0	37.0	37.0	37.0
25	36.317	37.0	37.0	37.0	37.0	37.0
26	36.2905	37.0	37.0	37.0	37.0	37.0
27	36.292	37.0	37.0	37.0	37.0	37.0
28	36.304	37.0	37.0	37.0	37.0	37.0
29	36.211	37.0	37.0	37.0	37.0	37.0
30	36.306	37.0	37.0	37.0	37.0	37.0
31	36.1175	37.0	37.0	37.0	37.0	37.0
32	36.1635	37.0	37.0	37.0	37.0	37.0
33	36.1545	37.0	37.0	37.0	37.0	37.0
34	36.1625	37.0	37.0	37.0	37.0	37.0
35	36.0405	37.0	37.0	37.0	37.0	37.0
36	36.1415	37.0	37.0	37.0	37.0	37.0
37	36.117	37.0	37.0	37.0	37.0	37.0
38	36.269	37.0	37.0	37.0	37.0	37.0
39	36.168	37.0	37.0	37.0	37.0	37.0
40	36.144	37.0	37.0	37.0	37.0	37.0
41	36.1085	37.0	37.0	37.0	37.0	37.0
42	36.0635	37.0	37.0	37.0	37.0	37.0
43	36.1115	37.0	37.0	37.0	37.0	37.0
44	36.1325	37.0	37.0	37.0	37.0	37.0
45	36.2695	37.0	37.0	37.0	37.0	37.0
46	36.1065	37.0	37.0	37.0	37.0	37.0
47	36.087	37.0	37.0	37.0	37.0	37.0
48	36.0095	37.0	37.0	37.0	37.0	37.0
49	36.098	37.0	37.0	37.0	37.0	37.0
50	36.058	37.0	37.0	37.0	37.0	37.0
51	35.952	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10	0.0
1101	11	0.0
1101	12	0.0
1101	13	0.0
1101	14	0.0
1101	15	0.0
1101	16	0.0
1101	17	0.0
1101	18	0.0
1101	19	0.0
1101	20	0.0
1101	21	0.0
1101	22	0.0
1101	23	0.0
1101	24	0.0
1101	25	0.0
1101	26	0.0
1101	27	0.0
1101	28	0.0
1101	29	0.0
1101	30	0.0
1101	31	0.0
1101	32	0.0
1101	33	0.0
1101	34	0.0
1101	35	0.0
1101	36	0.0
1101	37	0.0
1101	38	0.0
1101	39	0.0
1101	40	0.0
1101	41	0.0
1101	42	0.0
1101	43	0.0
1101	44	0.0
1101	45	0.0
1101	46	0.0
1101	47	0.0
1101	48	0.0
1101	49	0.0
1101	50	0.0
1101	51	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	1.0
23	2.0
24	4.0
25	2.0
26	2.0
27	8.0
28	7.0
29	8.0
30	14.0
31	26.0
32	41.0
33	76.0
34	128.0
35	532.0
36	2135.0
37	1012.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.575	17.675	12.975	40.775
2	27.025	21.8	36.4	14.774999999999999
3	20.9	26.375	29.325000000000003	23.400000000000002
4	23.425	35.35	22.7	18.525
5	27.0	34.425	23.175	15.4
6	24.099999999999998	36.85	21.025	18.025
7	22.575	21.925	38.224999999999994	17.275
8	23.25	21.975	30.599999999999998	24.175
9	23.674999999999997	22.8	33.825	19.7
10	19.5	37.974999999999994	23.849999999999998	18.675
11	26.325	27.224999999999998	22.875	23.575
12	28.875	22.7	25.724999999999998	22.7
13	24.45	27.500000000000004	26.0	22.05
14	25.1	29.7	27.35	17.849999999999998
15	23.599999999999998	31.0	25.900000000000002	19.5
16	25.275	26.775	28.4	19.55
17	24.95	29.925	25.275	19.85
18	28.075	28.749999999999996	24.675	18.5
19	24.099999999999998	29.299999999999997	25.05	21.55
20	23.1	31.775	27.525	17.599999999999998
21	23.375	30.225	28.349999999999998	18.05
22	25.0	28.925	28.275	17.8
23	26.174999999999997	29.4	26.05	18.375
24	25.275	30.65	25.45	18.625
25	23.65	29.625	27.575	19.15
26	27.425	29.15	25.3	18.125
27	26.0	28.625	26.974999999999998	18.4
28	25.1	29.549999999999997	26.3	19.05
29	25.424999999999997	29.475	25.124999999999996	19.975
30	24.625	28.675	26.575	20.125
31	26.125	29.4	24.625	19.85
32	24.775	29.549999999999997	25.074999999999996	20.599999999999998
33	26.174999999999997	29.849999999999998	25.75	18.224999999999998
34	23.225	31.175000000000004	25.324999999999996	20.275000000000002
35	24.474999999999998	32.9	26.174999999999997	16.45
36	24.625	28.025	28.449999999999996	18.9
37	24.775	28.525	26.674999999999997	20.025000000000002
38	26.075	27.500000000000004	26.924999999999997	19.5
39	27.650000000000002	29.7	23.0	19.650000000000002
40	22.650000000000002	29.475	25.775	22.1
41	26.8	30.0	25.35	17.849999999999998
42	23.275000000000002	28.95	25.924999999999997	21.85
43	24.95	31.624999999999996	25.4	18.025
44	24.099999999999998	28.675	26.424999999999997	20.8
45	23.5	28.9	27.925	19.675
46	25.374999999999996	28.95	26.575	19.1
47	23.35	28.925	28.575	19.15
48	25.3	28.775000000000002	26.0	19.925
49	21.85	31.95	27.425	18.775
50	27.175	28.7	26.375	17.75
51	25.575	29.849999999999998	29.349999999999998	15.225
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	0.5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	2.0
17	1.0
18	0.0
19	1.5
20	3.0
21	7.0
22	11.0
23	10.5
24	10.0
25	17.0
26	31.5
27	39.0
28	50.0
29	61.0
30	81.0
31	101.0
32	106.5
33	112.0
34	143.0
35	174.0
36	213.5
37	253.0
38	260.5
39	268.0
40	294.0
41	320.0
42	409.0
43	498.0
44	493.0
45	488.0
46	459.0
47	430.0
48	373.0
49	316.0
50	292.5
51	269.0
52	223.0
53	177.0
54	157.0
55	137.0
56	136.5
57	136.0
58	106.5
59	77.0
60	56.0
61	35.0
62	31.5
63	28.0
64	20.5
65	13.0
66	12.5
67	12.0
68	7.0
69	2.0
70	1.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.14999999999999
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.63055339049104	49.8
2	12.314886983632112	15.8
3	4.208885424785659	8.1
4	2.6110678098207325	6.7
5	1.0911925175370227	3.5000000000000004
6	0.46765393608729544	1.7999999999999998
7	0.5066250974279034	2.275
8	0.19485580670303976	1.0
9	0.23382696804364772	1.35
>10	0.7404520654715511	9.675
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTG	40	1.0	No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG	35	0.8750000000000001	No Hit
CTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGA	31	0.775	No Hit
CAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA	31	0.775	No Hit
CTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAAGT	29	0.7250000000000001	No Hit
CGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATT	29	0.7250000000000001	No Hit
CTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGT	28	0.7000000000000001	No Hit
CGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGT	23	0.575	No Hit
GTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGA	20	0.5	No Hit
TCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTG	18	0.44999999999999996	No Hit
CACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGAT	16	0.4	No Hit
GGTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAA	13	0.325	No Hit
CTTGGATTTATTGAAGACTAACTACTGCGAAAGCATTTGCCAAGGACGTTT	12	0.3	No Hit
CTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAAA	11	0.27499999999999997	No Hit
GGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATG	11	0.27499999999999997	No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT	10	0.25	No Hit
GGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAAAAGCCCCCTTGATTTT	10	0.25	No Hit
CTAGTACCGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAGAAGG	10	0.25	No Hit
GTCTAACGTCTATGCGAGTGTTTGGGTGTAAAACCCATACGCGTAATGAAA	10	0.25	No Hit
CGCAGATGTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACA	9	0.22499999999999998	No Hit
GTAAAACCCATACGCGTAATGAAAGTGAACGTAGGTTGGGGCCTCGCAAGA	9	0.22499999999999998	No Hit
AGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAA	9	0.22499999999999998	No Hit
ACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGG	9	0.22499999999999998	No Hit
CAGATGTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAA	9	0.22499999999999998	No Hit
CCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAA	9	0.22499999999999998	No Hit
ACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATT	8	0.2	No Hit
GGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAAAAGCC	8	0.2	No Hit
GTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGT	8	0.2	No Hit
CGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCG	8	0.2	No Hit
ATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTATGCCT	8	0.2	No Hit
GTGATTTGTCTGCTTAATTGCGATAACGAACGAGACCTTAACCTACTAAAT	7	0.17500000000000002	No Hit
GTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATA	7	0.17500000000000002	No Hit
CGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTT	7	0.17500000000000002	No Hit
CACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTGT	7	0.17500000000000002	No Hit
CAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCTA	7	0.17500000000000002	No Hit
GTTCATTCGGATAATTGGTTTTTGCGGCTGTCTGATCAGGCATTGCCGCGA	7	0.17500000000000002	No Hit
ACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCTT	7	0.17500000000000002	No Hit
CGATTGAATGGCTTAGTGAGGCCTCAGGATCTGCTTAGAGAAGGGGGCAAC	7	0.17500000000000002	No Hit
GTCAGAATCCATGCTAGAACGCGGTGATTTCTTTGCTCCACACAATATAGA	7	0.17500000000000002	No Hit
CAGAAATCTCCAGTAGAACAAAAGGGTAAAAGCCCCCTTGATTTTGATTTT	7	0.17500000000000002	No Hit
ATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAG	7	0.17500000000000002	No Hit
CTTTGGGCCCGGTTGGCCGGTCCGATTTTTTCGTGTACTGGATTTCCAACG	7	0.17500000000000002	No Hit
GGAAACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTT	7	0.17500000000000002	No Hit
AAGAAATTCAACCAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTT	6	0.15	No Hit
CGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCG	6	0.15	No Hit
CCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGA	6	0.15	No Hit
AGATGTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAA	6	0.15	No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA	6	0.15	No Hit
GCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTG	6	0.15	No Hit
GAACAGTTCATTCGGATAATTGGTTTTTGCGGCTGTCTGATCAGGCATTGC	6	0.15	No Hit
CAAACTTTAAATATGTAAGAAGTCCTTGTTACTTAATTGAACGTGGACATT	6	0.15	No Hit
GTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGCGGGAGTAACTAT	6	0.15	No Hit
GTAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAAC	6	0.15	No Hit
CCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCGGGA	6	0.15	No Hit
GTCAGAAAGTGATGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGT	6	0.15	No Hit
ACACAATAAGGATTGACAGATTGAGAGCTCTTTCTTGATTTTGTGGGTGGT	5	0.125	No Hit
CCACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTG	5	0.125	No Hit
GTGAGGGCCTTGGTCAGACGCAGCGGGCGTGCTTGTGGACTGCTTGGTGGG	5	0.125	No Hit
CCACTTCTTAGAGGGACTATCGGTTTCAAGCCGATGGAAGTTTGAGGCAAT	5	0.125	No Hit
CTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTG	5	0.125	No Hit
GATTTGTCTGCTTAATTGCGATAACGAACGAGACCTTAACCTACTAAATAG	5	0.125	No Hit
AACTCACCAGGTCCAGACACAATAAGGATTGACAGATTGAGAGCTCTTTCT	5	0.125	No Hit
GTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAA	5	0.125	No Hit
CTTATTCAATGAAGCGGAGCTGGAATTCATTTTCCACGTTCTAGCATTCAA	5	0.125	No Hit
AGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAA	5	0.125	No Hit
AGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACC	5	0.125	No Hit
CTTCGATGTCGGCTCTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTG	5	0.125	No Hit
GGCTTAGTGAGGCCTCAGGATCTGCTTAGAGAAGGGGGCAACTCCATCTCA	5	0.125	No Hit
GTTTATTTGATAGTTCCTTTACTACATGGTATAACTGTGGTAATTCTAGAG	5	0.125	No Hit
CACTACCTTTATAGTTTCTTTACTTATTCAATGAAGCGGAGCTGGAATTCA	5	0.125	No Hit
CTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAAAAGCCCCCTTG	5	0.125	No Hit
ATTGGCTCTAAGGGTCGGGTAGTGAGGGCCTTGGTCAGACGCAGCGGGCGT	5	0.125	No Hit
AGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAG	5	0.125	No Hit
CGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTGC	5	0.125	No Hit
GTGTAAAACCCATACGCGTAATGAAAGTGAACGTAGGTTGGGGCCTCGCAA	5	0.125	No Hit
CAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGATT	5	0.125	No Hit
GTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACCG	5	0.125	No Hit
GTGTTTTGTGCCCTCTGCTCCTTGTGGGTAGGGGAATCTCGCATTTCACTG	5	0.125	No Hit
TAGGAGTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACC	5	0.125	No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGCTTAATTGCGATAACGAACG	5	0.125	No Hit
GTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGACGTTCTGGGCCGCACG	5	0.125	No Hit
AAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAAAAG	5	0.125	No Hit
GTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.025	0.0	0.0	0.0	0.0
7	0.025	0.0	0.0	0.0	0.0
8	0.025	0.0	0.0	0.0	0.0
9	0.025	0.0	0.0	0.0	0.0
10	0.025	0.0	0.0	0.0	0.0
11	0.025	0.0	0.0	0.0	0.0
12	0.025	0.0	0.0	0.0	0.0
13	0.025	0.0	0.0	0.0	0.0
14	0.025	0.0	0.0	0.0	0.0
15	0.025	0.0	0.0	0.0	0.0
16	0.025	0.0	0.0	0.0	0.0
17	0.025	0.0	0.0	0.0	0.0
18	0.025	0.0	0.0	0.0	0.0
19	0.025	0.0	0.0	0.0	0.0
20	0.025	0.0	0.0	0.0	0.0
21	0.025	0.0	0.0	0.0	0.0
22	0.025	0.0	0.0	0.0	0.0
23	0.025	0.0	0.0	0.0	0.0
24	0.025	0.0	0.0	0.0	0.0
25	0.025	0.0	0.0	0.0	0.0
26	0.025	0.0	0.0	0.0	0.0
27	0.025	0.0	0.0	0.0	0.0
28	0.025	0.0	0.0	0.0	0.0
29	0.025	0.0	0.0	0.0	0.0
30	0.025	0.0	0.0	0.0	0.0
31	0.025	0.0	0.0	0.0	0.0
32	0.025	0.0	0.0	0.0	0.0
33	0.025	0.0	0.0	0.0	0.0
34	0.025	0.0	0.0	0.0	0.0
35	0.025	0.0	0.0	0.0	0.0
36	0.025	0.0	0.0	0.0	0.0
37	0.025	0.0	0.0	0.0	0.0
38	0.025	0.0	0.0	0.0	0.0
39	0.025	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Read 1691488 spots for SRR27822271.sra
Written 1691488 spots for SRR27822271.sra
Read 1691488 spots for SRR27822271.sra
Written 1691488 spots for SRR27822271.sra
Read 1691494 spots for SRR27822271.sra
Written 1691494 spots for SRR27822271.sra
Read 1691488 spots for SRR27822271.sra
Written 1691488 spots for SRR27822271.sra
Read 1691488 spots for SRR27822271.sra
Written 1691488 spots for SRR27822271.sra
Read 1691488 spots for SRR27822271.sra
Written 1691488 spots for SRR27822271.sra
Read 1691488 spots for SRR27822271.sra
Written 1691488 spots for SRR27822271.sra
Read 1691488 spots for SRR27822271.sra
Written 1691488 spots for SRR27822271.sra
SRR ids: ['SRR27822271.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_shws3dba
SRR27822271.sra spots: 13531910
blocks: [[1, 1691488], [1691489, 3382976], [3382977, 5074464], [5074465, 6765952], [6765953, 8457440], [8457441, 10148928], [10148929, 11840416], [11840417, 13531910]]
SRR27822271 file size 2334300
SRR27822271 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	/usr/local/bin/skewer -f sanger -l 18 -q 10 -k inf -t 8 -o SRR27822271 SRR27822271_1.fastq SRR27822271_2.fastq
Input file:	SRR27822271_1.fastq
Paired file:	SRR27822271_2.fastq
trimmed:	SRR27822271-trimmed-pair1.fastq, SRR27822271-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	8
Thu May 14 08:00:16 2026 >> started

Thu May 14 08:00:24 2026 >> done (7.380s)
13531910 read pairs processed; of these:
      82 ( 0.00%) short read pairs filtered out after trimming by size control
    1064 ( 0.01%) empty read pairs filtered out after trimming by size control
13530764 (99.99%) read pairs available; of these:
    3580 ( 0.03%) trimmed read pairs available after processing
13527184 (99.97%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	       7	  0.00%
 19	      84	  0.00%
 20	      16	  0.00%
 21	       7	  0.00%
 22	       5	  0.00%
 23	       9	  0.00%
 24	       7	  0.00%
 25	      18	  0.00%
 26	       5	  0.00%
 27	      22	  0.00%
 28	      11	  0.00%
 29	      10	  0.00%
 30	      18	  0.00%
 31	      13	  0.00%
 32	      18	  0.00%
 33	      29	  0.00%
 34	      25	  0.00%
 35	      42	  0.00%
 36	      59	  0.00%
 37	      56	  0.00%
 38	      78	  0.00%
 39	      70	  0.00%
 40	     133	  0.00%
 41	     133	  0.00%
 42	     189	  0.00%
 43	     189	  0.00%
 44	     214	  0.00%
 45	     215	  0.00%
 46	     226	  0.00%
 47	     352	  0.00%
 48	     373	  0.00%
 49	     445	  0.00%
 50	     502	  0.00%
 51	13527184	 99.97%
13530764 reads passed initial QC


criterion=sequence-density
sequence-density=0.26
sequence-density-rank=1
fanout-score=2.55
fanout-score-rank=8
prefix-density=0.24
prefix-fanout=2.6
sequence=ACGCTTGACTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGGCACCTCTAGCCTCAAATTCCGAGGGACTAAAGGATCGATAGGCCACACTTTCATGGTTTGTATTCACACTGAAAATCAAAATCAAGGGGGCTTTTACCCTTTTGTTCTACTGGAGATTTCTGTTCTCCATGAGCCCCCCTTAGGACATCTGCGTTATCGTTT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=30
fanout-score=16.27
fanout-score-rank=1
prefix-density=0.06
prefix-fanout=2.4
sequence=ACCTTCTTGATAAAGTTGGAGGTTTCCTTGACAATTTCTTGGAATCTGGATTCGTCCCATTTGACGGAGTCCATCTTGTTGACAGCAACAATCAATTGTCTAACACCCAAGGTGAAAGCCAACAAAGCGTGTTCTCTGGTTTGACCATCCTTAGAGATACCGGCTTCGAATTCACCGACACCACCAGCAATAATCAAGATAGCACAGTCAGCTTGAGAAGTACCAGTAATCATGTTCTTGATGAAATCTCTGTGACCTGGAGCATCAATAACGGTAACTTGGTACTTTGGAGTTTCGAACTTCCACAAAGCAATATCGATAGTGATACCTCTTTCTCTTTCAGCCTTTAACTTGTCCAAAACCCAAGCGTACTTGAAAGAACCCTTACCTAATTCAGCGGCTTCCTTTTCGAACTTTTCGATGGTTCTCTTGTCAATACCACCACACTTGTA


criterion=sequence-density
sequence-density=0.26
sequence-density-rank=1
fanout-score=2.69
fanout-score-rank=17
prefix-density=0.27
prefix-fanout=2.6
sequence=CATAGCGACATTGCTTTTTGATTCTTCGATGTCGGCTCTTCCTATCATACCGAAGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGAATGTTACCGCAATAGTAATTGAACTTAGTACGAGAGGAACAGTTCATTCGGATAATTGGTTTTTGCGGCTGTCTGATCAGGCATTGCCGCGAAGCTACCATCCGCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCGGTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGAAAGGCCTTGGGTGCTTGCTGGCGAATTGCAATGTCATTTTGCGTGGGGATAAATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGT


criterion=fanout-score
sequence-density=0.04
sequence-density-rank=6
fanout-score=64.92
fanout-score-rank=1
prefix-density=2.54
prefix-fanout=1.0
sequence=GTAAAACCCATCCGCGTAATGAA
SRR27822271 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	May 14 08:00:45
                             Started mapping on |	May 14 08:00:45
                                    Finished on |	May 14 08:01:06
       Mapping speed, Million of reads per hour |	2319.56

                          Number of input reads |	13530764
                      Average input read length |	101
                                    UNIQUE READS:
                   Uniquely mapped reads number |	3577770
                        Uniquely mapped reads % |	26.44%
                          Average mapped length |	101.85
                       Number of splices: Total |	87934
            Number of splices: Annotated (sjdb) |	79821
                       Number of splices: GT/AG |	85302
                       Number of splices: GC/AG |	181
                       Number of splices: AT/AC |	86
               Number of splices: Non-canonical |	2365
                      Mismatch rate per base, % |	0.08%
                         Deletion rate per base |	0.00%
                        Deletion average length |	1.28
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.09
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	9818726
             % of reads mapped to multiple loci |	72.57%
        Number of reads mapped to too many loci |	78102
             % of reads mapped to too many loci |	0.58%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	0.41%
                     % of reads unmapped: other |	0.00%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	134268	134268	134268
N_multimapping	9818726	9818726	9818726
N_noFeature	133295	3332639	202586
N_ambiguous	208654	1801	34679
UnstrandedReadsAssigned:3235821 PositiveStrandReadsAssigned:243330 NegativeStrandReadsAssigned:3340505
Dataset is classified negative stranded
MeadianReadLen=51 20thPercentileLength=51 echo kmer=47
SRR27822271 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 6,713
[index] number of k-mers: 8,283,195
[index] number of equivalence classes: 8,710
[quant] running in paired-end mode
[quant] will process pair 1: SRR27822271-trimmed-pair1.fastq
                             SRR27822271-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 13,530,764 reads, 3,696,579 reads pseudoaligned
[quant] estimated average fragment length: 232.13
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 844 rounds

  6714 SRR27822271.ke.tsv
  7126 SRR27822271.se.tsv
 13840 total
==> SRR27822271.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
YHR055C	186	53.0655	261.5	372.727
YPR161C	1974	1742.87	343	14.8854
YOL138C	4026	3794.87	408	8.13196
YDR395W	2835	2603.87	1040	30.2097
YGR129W	648	419.921	110	19.8133
YPR165W	630	402.676	877	164.731
YPR098C	486	269.38	111	31.1666
YPL015C	1074	842.87	295	26.4724
YCL050C	966	734.87	713	73.3857

==> SRR27822271.se.tsv <==
YDL248W	35
YDL247W-A	0
YDL247W	0
YDL246C	0
YDL245C	2
YDL244W	6
YDL243C	67
YDL242W	3
YDL241W	23
YDL240C-A	0
SRR27822271 completed mapping pipeline successfully
