Starting /dee2/code/volunteer_pipeline.sh SRR11699270
    current disk space = 2472635404288
    free memory = 1579613016 
SRR11699270 SRAfilesize
f284741f8440ff6f169c3a7a32143963  SRR11699270.sra
SRR11699270.sra file validated
SRR11699270 is single end
SRR11699270 is conventional basespace
SRR11699270 read1 length is 76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR11699270_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	76
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1705	32.0	32.0	32.0	32.0	32.0
2	31.38725	32.0	32.0	32.0	32.0	32.0
3	31.5615	32.0	32.0	32.0	32.0	32.0
4	31.5135	32.0	32.0	32.0	32.0	32.0
5	31.53175	32.0	32.0	32.0	32.0	32.0
6	35.041	36.0	36.0	36.0	36.0	36.0
7	35.07775	36.0	36.0	36.0	36.0	36.0
8	35.02	36.0	36.0	36.0	36.0	36.0
9	34.854	36.0	36.0	36.0	32.0	36.0
10-11	35.046125	36.0	36.0	36.0	36.0	36.0
12-13	35.08862499999999	36.0	36.0	36.0	36.0	36.0
14-15	35.02475	36.0	36.0	36.0	36.0	36.0
16-17	35.153625	36.0	36.0	36.0	36.0	36.0
18-19	35.121	36.0	36.0	36.0	36.0	36.0
20-21	34.905	36.0	36.0	36.0	36.0	36.0
22-23	34.978875	36.0	36.0	36.0	36.0	36.0
24-25	34.898125	36.0	36.0	36.0	36.0	36.0
26-27	34.869749999999996	36.0	36.0	36.0	34.0	36.0
28-29	34.920625	36.0	36.0	36.0	36.0	36.0
30-31	34.820750000000004	36.0	36.0	36.0	34.0	36.0
32-33	34.8535	36.0	36.0	36.0	34.0	36.0
34-35	34.864374999999995	36.0	36.0	36.0	36.0	36.0
36-37	34.992999999999995	36.0	36.0	36.0	36.0	36.0
38-39	34.9065	36.0	36.0	36.0	34.0	36.0
40-41	34.876	36.0	36.0	36.0	36.0	36.0
42-43	34.88725	36.0	36.0	36.0	34.0	36.0
44-45	34.8735	36.0	36.0	36.0	36.0	36.0
46-47	34.766875	36.0	36.0	36.0	34.0	36.0
48-49	34.616625	36.0	36.0	36.0	34.0	36.0
50-51	34.219375	36.0	36.0	36.0	32.0	36.0
52-53	33.935125	36.0	36.0	36.0	32.0	36.0
54-55	33.42175	36.0	36.0	36.0	20.5	36.0
56-57	32.412125	36.0	32.0	36.0	14.0	36.0
58-59	31.571875	36.0	32.0	36.0	14.0	36.0
60-61	30.488875	36.0	29.5	36.0	14.0	36.0
62-63	29.546125	36.0	20.5	36.0	14.0	36.0
64-65	27.95125	32.0	14.0	36.0	14.0	36.0
66-67	25.421	27.0	14.0	36.0	14.0	36.0
68-69	24.06225	20.5	14.0	36.0	14.0	36.0
70-71	23.51875	20.5	14.0	36.0	14.0	36.0
72-73	23.099375000000002	17.5	14.0	36.0	14.0	36.0
74-75	22.567	14.0	14.0	34.0	14.0	36.0
76	22.2025	14.0	14.0	32.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	5.0
23	6.0
24	22.0
25	29.0
26	60.0
27	83.0
28	160.0
29	240.0
30	378.0
31	445.0
32	616.0
33	847.0
34	712.0
35	395.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.799999999999997	31.65	23.525	21.025
2	23.625	27.775	25.95	22.650000000000002
3	24.425	31.25	19.825	24.5
4	30.925000000000004	29.95	18.975	20.150000000000002
5	22.825	25.900000000000002	22.725	28.549999999999997
6	27.325	26.8	26.150000000000002	19.725
7	30.85	33.225	19.1	16.825000000000003
8	22.900000000000002	24.575	31.075000000000003	21.45
9	24.775	31.15	21.375	22.7
10-11	31.924999999999997	27.525	22.2125	18.337500000000002
12-13	23.45	22.0625	24.6	29.8875
14-15	29.4875	36.8375	18.025	15.65
16-17	24.75	24.212500000000002	31.2875	19.75
18-19	37.0375	24.65	22.237499999999997	16.075
20-21	24.0375	33.800000000000004	24.474999999999998	17.6875
22-23	32.6125	36.6375	14.1375	16.6125
24-25	28.725	38.737500000000004	15.225	17.3125
26-27	29.1625	32.087500000000006	18.0125	20.7375
28-29	31.15	28.999999999999996	16.662499999999998	23.1875
30-31	32.737500000000004	35.175	10.6	21.4875
32-33	29.062500000000004	36.9375	12.2875	21.712500000000002
34-35	29.775000000000002	30.7625	15.125	24.337500000000002
36-37	26.187500000000004	33.4125	12.174999999999999	28.225
38-39	23.125	33.6	14.174999999999999	29.099999999999998
40-41	23.549999999999997	28.799999999999997	17.712500000000002	29.9375
42-43	26.424999999999997	25.7375	20.575	27.2625
44-45	21.1125	32.375	16.775000000000002	29.7375
46-47	20.95	30.099999999999998	17.1125	31.837500000000002
48-49	15.187500000000002	26.5625	18.3625	39.887499999999996
50-51	14.2875	25.2625	21.2625	39.1875
52-53	18.15	21.337500000000002	22.025	38.487500000000004
54-55	17.837500000000002	21.75	24.7875	35.625
56-57	13.925	19.6	26.375	40.1
58-59	15.162500000000001	18.1625	25.587500000000002	41.0875
60-61	19.0625	15.437500000000002	26.950000000000003	38.550000000000004
62-63	17.05	17.849999999999998	29.95	35.15
64-65	13.112499999999999	17.0875	30.6875	39.1125
66-67	15.562500000000002	12.075	33.675	38.6875
68-69	17.0125	12.812499999999998	36.199999999999996	33.975
70-71	17.5875	12.0375	38.025	32.35
72-73	15.262500000000001	11.5625	36.7375	36.4375
74-75	17.8625	9.4125	36.5625	36.162499999999994
76	12.825000000000001	8.55	42.975	35.65
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.5
39	2.0
40	4.0
41	9.0
42	13.0
43	20.5
44	40.0
45	95.5
46	139.0
47	222.5
48	425.5
49	628.5
50	712.0
51	647.0
52	561.0
53	489.5
54	439.0
55	348.0
56	205.0
57	127.0
58	101.0
59	82.0
60	47.0
61	20.5
62	10.0
63	8.5
64	6.0
65	4.0
66	2.0
67	1.0
68	0.5
69	0.5
70	0.5
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.09140767824498	61.6
2	4.2778793418647165	5.8500000000000005
3	2.010968921389397	4.125
4	0.9506398537477149	2.6
5	0.5484460694698354	1.875
6	0.47531992687385743	1.95
7	0.3290676416819013	1.575
8	0.10968921389396709	0.6
9	0.14625228519195613	0.8999999999999999
>10	0.9872029250457038	12.425
>50	0.03656307129798903	1.6
>100	0.03656307129798903	4.9
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCC	196	4.9	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAAC	64	1.6	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCA	41	1.0250000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	33	0.8250000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	31	0.775	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCTAGATCGGAAGAGCA	28	0.7000000000000001	No Hit
AACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAG	28	0.7000000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCT	25	0.625	TruSeq Adapter, Index 6 (97% over 36bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTGAGATCGGAAGAGCAC	24	0.6	No Hit
AGGGCTATAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCC	23	0.575	TruSeq Adapter, Index 6 (97% over 36bp)
TAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCA	22	0.5499999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAATTCATGATCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	19	0.475	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCCTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACT	19	0.475	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACT	18	0.44999999999999996	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	17	0.42500000000000004	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGT	16	0.4	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTGCAT	16	0.4	TruSeq Adapter, Index 6 (97% over 36bp)
CCAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT	14	0.35000000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTGTAGATCGGAAGAGCA	14	0.35000000000000003	No Hit
GGGGATGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC	13	0.325	TruSeq Adapter, Index 5 (97% over 35bp)
GCGACCCCAGGTCAGTCGGGACTAGATCGGAAGAGCACACGTCTGAACTC	13	0.325	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTAGATCGGAAGAGCACA	12	0.3	No Hit
CTAACGAACGAACGATTTGAAGATCGGAAGAGCACACGTCTGAACTCCAG	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTGAGATCGGAAGAGCAC	10	0.25	No Hit
TCCTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTC	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCAGGATAGCTCAGTTGGTAGAGCAGAGGACTGAGATCGGAAGAGCACA	10	0.25	No Hit
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCC	10	0.25	TruSeq Adapter, Index 6 (97% over 36bp)
AGGGATGTAGCGCAGCTTGGTAGCGCTTTTGTTTTAGATCGGAAGAGCAC	10	0.25	No Hit
CGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC	9	0.22499999999999998	TruSeq Adapter, Index 5 (97% over 35bp)
TATTCTGGTGTCCTAGGCGTAGAGGAACCACACCAGATCGGAAGAGCACA	9	0.22499999999999998	No Hit
TGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGT	9	0.22499999999999998	No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACTCAGATCGGAAGAGCACACGTC	9	0.22499999999999998	No Hit
AACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGT	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGACACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACT	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATAGCTCGATGCCAGAATAGATCGGAAGAGCACACGTCTGAACTCCAG	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAGGTCCTGCGGCAAAATAGCTCGATGCCAGAATAGATCGGAAGAGCAC	7	0.17500000000000002	No Hit
TTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTGCATCG	7	0.17500000000000002	TruSeq Adapter, Index 6 (97% over 36bp)
TCTCATGGAGAGTTCGATCCTGGCTAGATCGGAAGAGCACACGTCTGAAC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGAGATCGGAAGAGCA	7	0.17500000000000002	No Hit
GAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTC	7	0.17500000000000002	No Hit
GGGCCTGTAGCTCAGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATGTATGCAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGG	7	0.17500000000000002	No Hit
CACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAAAAGGCTGACGCGGGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGGCAAAATAGCTCGATGCCAGAATAGATCGGAAGAGCACACGTCTGAA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCAGTGATGTATGCAGCTGAGGCATCCTAACGAACGAACGATTTGAACAG	6	0.15	No Hit
TCCAGCCCCGCGTCGCACGGATTCGTAGATCGGAAGAGCACACGTCTGAA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCAGATCGGAAGAGCACACGTC	6	0.15	No Hit
CTAACGAACGAACGATTTGAATAGATCGGAAGAGCACACGTCTGAACTCC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGTTGTAGTCTAGCTGGTTAGGATACTCAGATCGGAAGAGCACACGTC	6	0.15	No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTGTAGATCGGAAGAGCA	6	0.15	No Hit
ACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATTCTGGTGTCCTAGGCGTAGAGGAACCACAAGATCGGAAGAGCACACG	6	0.15	No Hit
GCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACAC	5	0.125	No Hit
GTGATGTATGCAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATC	5	0.125	No Hit
TCGCACGGATTCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC	5	0.125	TruSeq Adapter, Index 5 (97% over 35bp)
CGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTGC	5	0.125	TruSeq Adapter, Index 6 (97% over 36bp)
CGACACGACTCTCGGCAACGAGATCGGAAGAGCACACGTCTGAACTCCAG	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGTCGATGCCCGAGCGGTTAATGGGGACGGACTGAGATCGGAAGAGCAC	5	0.125	No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTGCTCTTAGATCGGAAGAGCAC	5	0.125	No Hit
TCCTGCGGCAAAATAGCTCGATGCCAGAATAGATCGGAAGAGCACACGTC	5	0.125	No Hit
TAATTCATGATCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTCGATGCCAGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTAGATCGGAAGAGC	5	0.125	No Hit
GCGACCCCAGGTCAGTCGGGACTACCCGCTAGATCGGAAGAGCACACGTC	5	0.125	No Hit
AGCGGAGTAGAGCAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCC	5	0.125	TruSeq Adapter, Index 6 (97% over 36bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.025	0.0	0.0	0.0	0.0
2	0.025	0.0	0.0	0.0	0.0
3	0.15	0.0	0.0	0.0	0.0
4	0.225	0.0	0.0	0.0	0.0
5	0.3	0.0	0.0	0.0	0.0
6	0.45	0.0	0.0	0.0	0.0
7	0.65	0.0	0.0	0.0	0.0
8	0.9	0.0	0.0	0.0	0.0
9	1.575	0.0	0.0	0.0	0.0
10	2.125	0.0	0.0	0.0	0.0
11	2.8	0.0	0.0	0.0	0.0
12	3.675	0.0	0.0	0.0	0.0
13	5.125	0.0	0.0	0.0	0.0
14	7.4	0.0	0.0	0.0	0.0
15	9.675	0.0	0.0	0.0	0.0
16	11.65	0.0	0.0	0.0	0.0
17	15.4	0.0	0.0	0.0	0.0
18	18.3	0.0	0.0	0.0	0.0
19	20.575	0.0	0.0	0.0	0.0
20	23.875	0.0	0.0	0.0	0.0
21	27.8	0.0	0.0	0.0	0.0
22	33.4	0.0	0.0	0.0	0.0
23	42.05	0.0	0.0	0.0	0.0
24	45.575	0.0	0.0	0.0	0.0
25	53.475	0.0	0.0	0.0	0.0
26	57.525	0.0	0.0	0.0	0.0
27	60.0	0.0	0.0	0.0	0.0
28	63.2	0.0	0.0	0.0	0.0
29	65.175	0.0	0.0	0.0	0.0
30	67.275	0.0	0.0	0.0	0.0
31	70.525	0.0	0.0	0.0	0.0
32	73.025	0.0	0.0	0.0	0.0
33	75.25	0.0	0.0	0.0	0.0
34	77.475	0.0	0.0	0.0	0.0
35	80.375	0.0	0.0	0.0	0.0
36	83.875	0.0	0.0	0.0	0.0
37	87.25	0.0	0.0	0.0	0.0
38	88.95	0.0	0.0	0.0	0.0
39	89.975	0.0	0.0	0.0	0.0
40	90.95	0.0	0.0	0.0	0.0
41	91.725	0.0	0.0	0.0	0.0
42	92.25	0.0	0.0	0.0	0.0
43	92.85	0.0	0.0	0.0	0.0
44	93.35	0.0	0.0	0.0	0.0
45	93.625	0.0	0.0	0.0	0.0
46	93.925	0.0	0.0	0.0	0.0
47	94.125	0.0	0.0	0.0	0.0
48	94.325	0.0	0.0	0.0	0.0
49	94.7	0.0	0.0	0.0	0.0
50	94.8	0.0	0.0	0.0	0.0
51	94.85	0.0	0.0	0.0	0.0
52	94.9	0.0	0.0	0.0	0.0
53	94.95	0.0	0.0	0.0	0.0
54	94.95	0.0	0.0	0.0	0.0
55	94.975	0.0	0.0	0.0	0.0
56	94.975	0.0	0.0	0.0	0.0
57	94.975	0.0	0.0	0.0	0.0
58	94.975	0.0	0.0	0.0	0.0
59	94.975	0.0	0.0	0.0	0.0
60	95.0	0.0	0.0	0.0	0.0
61	95.025	0.0	0.0	0.0	0.0
62	95.025	0.0	0.0	0.0	0.0
63	95.025	0.0	0.0	0.0	0.0
64	95.025	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCT	15	0.0020658788	70.00001	11
AGGATAT	15	0.0020658788	70.00001	22
GCTGGTC	15	0.0020658788	70.00001	15
TAGCTGG	15	0.0020658788	70.00001	13
CTAGCTG	15	0.0020658788	70.00001	12
GTCTAGC	15	0.0020658788	70.00001	10
AGCTGGT	15	0.0020658788	70.00001	14
TGGTCAG	25	2.3570374E-6	70.0	17
TTGTAGT	25	2.3570374E-6	70.0	5
TCAGGAT	20	7.003785E-5	70.0	20
CCGTTGT	20	7.003785E-5	70.0	2
TAGTCTA	20	7.003785E-5	70.0	8
GGTCAGG	20	7.003785E-5	70.0	18
TGTAGTC	20	7.003785E-5	70.0	6
TCCGTTG	20	7.003785E-5	70.0	1
CGTTGTA	20	7.003785E-5	70.0	3
AGTCTAG	20	7.003785E-5	70.0	9
CAGGATA	20	7.003785E-5	70.0	21
GTCAGGA	20	7.003785E-5	70.0	19
GTAGTCT	20	7.003785E-5	70.0	7
>>END_MODULE
Rejected 149377 READS because READLEN < 1
Read 149377 spots for SRR11699270.sra
Written 149377 spots for SRR11699270.sra
Rejected 149377 READS because READLEN < 1
Read 149377 spots for SRR11699270.sra
Written 149377 spots for SRR11699270.sra
Rejected 149377 READS because READLEN < 1
Read 149377 spots for SRR11699270.sra
Written 149377 spots for SRR11699270.sra
Rejected 149377 READS because READLEN < 1
Read 149377 spots for SRR11699270.sra
Written 149377 spots for SRR11699270.sra
Rejected 149377 READS because READLEN < 1
Read 149377 spots for SRR11699270.sra
Written 149377 spots for SRR11699270.sra
Rejected 149377 READS because READLEN < 1
Read 149377 spots for SRR11699270.sra
Written 149377 spots for SRR11699270.sra
Rejected 149377 READS because READLEN < 1
Read 149377 spots for SRR11699270.sra
Written 149377 spots for SRR11699270.sra
Rejected 149377 READS because READLEN < 1
Read 149377 spots for SRR11699270.sra
Written 149377 spots for SRR11699270.sra
Rejected 149377 READS because READLEN < 1
Read 149377 spots for SRR11699270.sra
Written 149377 spots for SRR11699270.sra
Rejected 149377 READS because READLEN < 1
Read 149377 spots for SRR11699270.sra
Written 149377 spots for SRR11699270.sra
Rejected 149377 READS because READLEN < 1
Read 149377 spots for SRR11699270.sra
Written 149377 spots for SRR11699270.sra
Rejected 149377 READS because READLEN < 1
Read 149377 spots for SRR11699270.sra
Written 149377 spots for SRR11699270.sra
Rejected 149377 READS because READLEN < 1
Read 149377 spots for SRR11699270.sra
Written 149377 spots for SRR11699270.sra
Rejected 149377 READS because READLEN < 1
Read 149377 spots for SRR11699270.sra
Written 149377 spots for SRR11699270.sra
Rejected 149377 READS because READLEN < 1
Read 149377 spots for SRR11699270.sra
Written 149377 spots for SRR11699270.sra
Rejected 149377 READS because READLEN < 1
Read 149377 spots for SRR11699270.sra
Written 149377 spots for SRR11699270.sra
Rejected 149391 READS because READLEN < 1
Read 149391 spots for SRR11699270.sra
Written 149391 spots for SRR11699270.sra
Rejected 149377 READS because READLEN < 1
Read 149377 spots for SRR11699270.sra
Written 149377 spots for SRR11699270.sra
Rejected 149377 READS because READLEN < 1
Read 149377 spots for SRR11699270.sra
Written 149377 spots for SRR11699270.sra
Rejected 149377 READS because READLEN < 1
Read 149377 spots for SRR11699270.sra
Written 149377 spots for SRR11699270.sra
SRR ids: ['SRR11699270.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_xfa1q380
SRR11699270.sra spots: 2987554
blocks: [[1, 149377], [149378, 298754], [298755, 448131], [448132, 597508], [597509, 746885], [746886, 896262], [896263, 1045639], [1045640, 1195016], [1195017, 1344393], [1344394, 1493770], [1493771, 1643147], [1643148, 1792524], [1792525, 1941901], [1941902, 2091278], [2091279, 2240655], [2240656, 2390032], [2390033, 2539409], [2539410, 2688786], [2688787, 2838163], [2838164, 2987554]]
SRR11699270 file size 566749
SRR11699270 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR11699270 SRR11699270_1.fastq
Input file:	SRR11699270_1.fastq
trimmed:	SRR11699270-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	20
Wed Jun 18 05:52:00 2025 >> started

Wed Jun 18 05:52:01 2025 >> done (1.362s)
2987554 reads processed; of these:
 625136 (20.92%) short reads filtered out after trimming by size control
   1569 ( 0.05%) empty reads filtered out after trimming by size control
2360849 (79.02%) reads available; of these:
2305381 (97.65%) trimmed reads available after processing
  55468 ( 2.35%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  76607	  3.24%
 19	 115681	  4.90%
 20	 113623	  4.81%
 21	 166910	  7.07%
 22	 259889	 11.01%
 23	 102650	  4.35%
 24	 247895	 10.50%
 25	 117870	  4.99%
 26	  79195	  3.35%
 27	  82285	  3.49%
 28	  66745	  2.83%
 29	  70075	  2.97%
 30	 108499	  4.60%
 31	  68470	  2.90%
 32	  66303	  2.81%
 33	  73981	  3.13%
 34	  87030	  3.69%
 35	 112055	  4.75%
 36	 101492	  4.30%
 37	  43465	  1.84%
 38	  31365	  1.33%
 39	  27937	  1.18%
 40	  21944	  0.93%
 41	  23100	  0.98%
 42	  17041	  0.72%
 43	  13802	  0.58%
 44	   9467	  0.40%
 45	      0	  0.00%
 46	      0	  0.00%
 47	      0	  0.00%
 48	      0	  0.00%
 49	      0	  0.00%
 50	      0	  0.00%
 51	      0	  0.00%
 52	      0	  0.00%
 53	      0	  0.00%
 54	      0	  0.00%
 55	      0	  0.00%
 56	      0	  0.00%
 57	      0	  0.00%
 58	      0	  0.00%
 59	      0	  0.00%
 60	      0	  0.00%
 61	      0	  0.00%
 62	      0	  0.00%
 63	      0	  0.00%
 64	      0	  0.00%
 65	      0	  0.00%
 66	      0	  0.00%
 67	      0	  0.00%
 68	      0	  0.00%
 69	      0	  0.00%
 70	      0	  0.00%
 71	      0	  0.00%
 72	      2	  0.00%
 73	      0	  0.00%
 74	      1	  0.00%
 75	      2	  0.00%
 76	  55468	  2.35%
2360849 reads passed initial QC


criterion=sequence-density
sequence-density=3.98
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=17
prefix-density=0.02
prefix-fanout=1.0
sequence=TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCTCACCCGAAAGACCCGGGTT


criterion=fanout-score
sequence-density=0.34
sequence-density-rank=19
fanout-score=17.57
fanout-score-rank=1
prefix-density=1.30
prefix-fanout=4.6
sequence=GTCTAGTGGTATGATTCTCGCTT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	Jun 18 05:52:23
                             Started mapping on |	Jun 18 05:52:23
                                    Finished on |	Jun 18 05:52:35
       Mapping speed, Million of reads per hour |	708.25

                          Number of input reads |	2360849
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	246259
                        Uniquely mapped reads % |	10.43%
                          Average mapped length |	25.00
                       Number of splices: Total |	5855
            Number of splices: Annotated (sjdb) |	2989
                       Number of splices: GT/AG |	5425
                       Number of splices: GC/AG |	281
                       Number of splices: AT/AC |	2
               Number of splices: Non-canonical |	147
                      Mismatch rate per base, % |	1.15%
                         Deletion rate per base |	0.02%
                        Deletion average length |	1.48
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.51
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	282832
             % of reads mapped to multiple loci |	11.98%
        Number of reads mapped to too many loci |	571390
             % of reads mapped to too many loci |	24.20%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.65%
                     % of reads unmapped: other |	46.73%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	1831758	1831758	1831758
N_multimapping	282832	282832	282832
N_noFeature	189362	196927	236774
N_ambiguous	1966	33	15
UnstrandedReadsAssigned:54931 PositiveStrandReadsAssigned:49299 NegativeStrandReadsAssigned:9470
Dataset is classified positive stranded
MeadianReadLen=25 20thPercentileLength=21 echo kmer=19
SRR11699270 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 72,539
[index] number of k-mers: 56,563,136
[index] number of equivalence classes: 414,827
[quant] running in single-end mode
[quant] will process file 1: SRR11699270-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,360,849 reads, 340,857 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 850 rounds

  72540 SRR11699270.ke.tsv
  44303 SRR11699270.se.tsv
 116843 total
==> SRR11699270.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Zm00001eb096110_T001	2067	1968	0	0
Zm00001eb321680_T001	3417	3318	2	1.76928
Zm00001eb323640_T001	3273	3174	0	0
Zm00001eb314140_T001	893	794	0	0
Zm00001eb080260_T001	3529	3430	1	0.855754
Zm00001eb026490_T001	2433	2334	0	0
Zm00001eb026490_T002	2229	2130	0	0
Zm00001eb026490_T003	2220	2121	0	0
Zm00001eb281360_T001	2412	2313	1	1.26902

==> SRR11699270.se.tsv <==
Zm00001eb015280	0
Zm00001eb000610	0
Zm00001eb033210	0
Zm00001eb044610	0
Zm00001eb014360	0
Zm00001eb051320	1
Zm00001eb021860	0
Zm00001eb011690	0
Zm00001eb016840	0
Zm00001eb052060	0
SRR11699270 completed mapping pipeline successfully
