Starting /dee2/code/volunteer_pipeline.sh SRR11699302
    current disk space = 2466865479680
    free memory = 1581471236 
SRR11699302 SRAfilesize
37b9b6c2122d26e42c1f7087ea140b0e  SRR11699302.sra
SRR11699302.sra file validated
SRR11699302 is single end
SRR11699302 is conventional basespace
SRR11699302 read1 length is 76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR11699302_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26425	32.0	32.0	32.0	32.0	32.0
2	31.50925	32.0	32.0	32.0	32.0	32.0
3	31.54875	32.0	32.0	32.0	32.0	32.0
4	31.439	32.0	32.0	32.0	32.0	32.0
5	31.629	32.0	32.0	32.0	32.0	32.0
6	35.1	36.0	36.0	36.0	36.0	36.0
7	35.128	36.0	36.0	36.0	36.0	36.0
8	35.16225	36.0	36.0	36.0	36.0	36.0
9	34.9785	36.0	36.0	36.0	36.0	36.0
10-11	35.060125	36.0	36.0	36.0	36.0	36.0
12-13	35.176	36.0	36.0	36.0	36.0	36.0
14-15	35.107375000000005	36.0	36.0	36.0	36.0	36.0
16-17	35.206	36.0	36.0	36.0	36.0	36.0
18-19	35.18375	36.0	36.0	36.0	36.0	36.0
20-21	35.033500000000004	36.0	36.0	36.0	36.0	36.0
22-23	35.0515	36.0	36.0	36.0	36.0	36.0
24-25	35.102125	36.0	36.0	36.0	36.0	36.0
26-27	34.91975	36.0	36.0	36.0	36.0	36.0
28-29	34.980000000000004	36.0	36.0	36.0	36.0	36.0
30-31	34.947375	36.0	36.0	36.0	36.0	36.0
32-33	34.924875	36.0	36.0	36.0	36.0	36.0
34-35	35.017624999999995	36.0	36.0	36.0	36.0	36.0
36-37	34.89775	36.0	36.0	36.0	36.0	36.0
38-39	34.925375	36.0	36.0	36.0	36.0	36.0
40-41	34.93775	36.0	36.0	36.0	36.0	36.0
42-43	34.914375	36.0	36.0	36.0	36.0	36.0
44-45	34.84825	36.0	36.0	36.0	36.0	36.0
46-47	34.948125	36.0	36.0	36.0	36.0	36.0
48-49	34.772	36.0	36.0	36.0	34.0	36.0
50-51	34.615750000000006	36.0	36.0	36.0	32.0	36.0
52-53	34.48225	36.0	36.0	36.0	32.0	36.0
54-55	34.265	36.0	36.0	36.0	32.0	36.0
56-57	33.778	36.0	36.0	36.0	27.0	36.0
58-59	33.578625	36.0	36.0	36.0	27.0	36.0
60-61	33.602125	36.0	36.0	36.0	27.0	36.0
62-63	33.490750000000006	36.0	36.0	36.0	27.0	36.0
64-65	33.06225	36.0	32.0	36.0	27.0	36.0
66-67	32.373374999999996	36.0	32.0	36.0	20.5	36.0
68-69	32.781875	36.0	32.0	36.0	27.0	36.0
70-71	33.034875	36.0	32.0	36.0	27.0	36.0
72-73	32.6325	36.0	34.0	36.0	20.5	36.0
74-75	33.465625	36.0	36.0	36.0	27.0	36.0
76	30.6685	36.0	27.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	2.0
22	3.0
23	4.0
24	7.0
25	14.0
26	22.0
27	30.0
28	48.0
29	70.0
30	108.0
31	149.0
32	202.0
33	337.0
34	1060.0
35	1941.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.4	30.325000000000003	24.05	21.224999999999998
2	22.400000000000002	27.125	25.424999999999997	25.05
3	24.325	30.3	18.025	27.35
4	30.675	30.625000000000004	18.65	20.05
5	22.0	26.0	26.3	25.7
6	24.725	27.625	26.724999999999998	20.925
7	31.85	33.025	18.75	16.375
8	23.425	24.275	30.55	21.75
9	25.4	33.275	20.200000000000003	21.125
10-11	31.9625	26.825	23.35	17.8625
12-13	22.875	22.075	25.687500000000004	29.362500000000004
14-15	29.475	37.4	18.05	15.075
16-17	23.2625	24.099999999999998	31.724999999999998	20.9125
18-19	36.65	24.4375	22.0	16.9125
20-21	23.6625	32.487500000000004	26.3125	17.5375
22-23	31.8625	36.75	15.174999999999999	16.2125
24-25	29.525000000000002	38.987500000000004	15.0625	16.425
26-27	28.3125	33.3625	18.4	19.925
28-29	31.837500000000002	29.025000000000002	16.400000000000002	22.7375
30-31	33.537499999999994	35.325	10.212499999999999	20.925
32-33	29.225	38.3125	11.637500000000001	20.825
34-35	30.825000000000003	30.837500000000002	14.6875	23.65
36-37	28.512500000000003	33.725	11.475	26.2875
38-39	22.875	35.4125	13.200000000000001	28.512500000000003
40-41	25.112499999999997	29.6875	16.0125	29.1875
42-43	27.2625	26.0375	19.5625	27.1375
44-45	21.0625	32.225	17.1875	29.525000000000002
46-47	21.2625	31.412499999999998	16.1875	31.137500000000003
48-49	16.8625	27.1	20.325	35.712500000000006
50-51	14.099999999999998	26.7625	22.6	36.5375
52-53	19.037499999999998	23.7875	24.3875	32.7875
54-55	16.537499999999998	25.4375	27.537499999999998	30.4875
56-57	13.525	22.725	30.9	32.85
58-59	14.637500000000001	24.474999999999998	31.2625	29.625
60-61	14.762500000000001	21.5375	36.0875	27.6125
62-63	12.537499999999998	24.775	32.0125	30.675
64-65	12.375	20.849999999999998	33.45	33.324999999999996
66-67	16.537499999999998	15.312500000000002	37.2125	30.9375
68-69	16.3125	16.8125	36.95	29.925
70-71	19.2375	15.6375	38.224999999999994	26.900000000000002
72-73	18.375	14.7	37.762499999999996	29.1625
74-75	20.1125	10.9625	35.6875	33.2375
76	16.8	10.25	38.9	34.050000000000004
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.5
21	1.0
22	0.5
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	1.0
33	2.0
34	1.0
35	0.0
36	1.5
37	3.0
38	4.5
39	6.0
40	19.0
41	44.5
42	57.0
43	81.0
44	134.0
45	302.0
46	441.0
47	672.0
48	735.5
49	564.5
50	561.0
51	513.0
52	383.0
53	231.0
54	161.0
55	126.5
56	86.5
57	55.0
58	29.0
59	18.5
60	7.5
61	4.5
62	2.0
63	1.0
64	0.5
65	1.5
66	1.5
67	1.0
68	1.0
69	0.5
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.25
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.25650557620818	60.02499999999999
2	5.687732342007435	7.6499999999999995
3	1.5613382899628252	3.15
4	0.7434944237918215	2.0
5	0.7806691449814126	2.625
6	0.11152416356877323	0.44999999999999996
7	0.3345724907063197	1.575
8	0.26022304832713755	1.4000000000000001
9	0.18587360594795538	1.125
>10	0.966542750929368	12.9
>50	0.07434944237918216	3.2750000000000004
>100	0.03717472118959108	3.8249999999999997
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCC	153	3.8249999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAAC	79	1.975	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	52	1.3	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTGAGATCGGAAGAGCAC	41	1.0250000000000001	No Hit
CTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCA	36	0.8999999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACT	35	0.8750000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	35	0.8750000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTTGTAGTCTAGCTGGTCAGGATACTCAGATCGGAAGAGCACACGTC	32	0.8	No Hit
TAATTCATGATCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	30	0.75	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT	25	0.625	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGT	21	0.525	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAG	21	0.525	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGT	20	0.5	TruSeq Adapter, Index 13 (97% over 36bp)
AGGGCTATAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGT	19	0.475	TruSeq Adapter, Index 13 (97% over 36bp)
AACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTA	17	0.42500000000000004	TruSeq Adapter, Index 22 (97% over 37bp)
CACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACTCCA	16	0.4	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCTAGATCGGAAGAGCA	16	0.4	No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCAGATCGGAAGAGCACAC	16	0.4	No Hit
ATCCTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACT	15	0.375	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCA	14	0.35000000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCCGCCATGGTGAAATTGGTAGACACGCTGCTCTTAGATCGGAAGAGCAC	14	0.35000000000000003	No Hit
AACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	14	0.35000000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGACCCCAGGTCAGTCGGGACTAGATCGGAAGAGCACACGTCTGAACTC	13	0.325	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTATC	12	0.3	TruSeq Adapter, Index 22 (97% over 37bp)
CTAACGAACGAACGATTTGAAGATCGGAAGAGCACACGTCTGAACTCCAG	12	0.3	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCGTTGTAGTCTAGTTGGTCAGGATACTCAGATCGGAAGAGCACACGTC	12	0.3	No Hit
CGACACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACT	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCCTAACGAACGAACGATTTGAAGATCGGAAGAGCACACGTCTGAACTC	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCCCGCGTCGCACGGATTCGTAGATCGGAAGAGCACACGTCTGAACTCC	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCAGATCGGAAGAGCACACGTC	9	0.22499999999999998	No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTGAGATCGGAAGAGCAC	9	0.22499999999999998	No Hit
ATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTATCAT	9	0.22499999999999998	TruSeq Adapter, Index 22 (97% over 37bp)
TCCTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTC	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG	8	0.2	TruSeq Adapter, Index 2 (97% over 35bp)
CAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGATGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG	8	0.2	TruSeq Adapter, Index 2 (97% over 35bp)
TCCTCAGTAGCTCAGTGGTAGAGCGGTCGGCTGAGATCGGAAGAGCACAC	8	0.2	No Hit
GAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTC	8	0.2	No Hit
CAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCAC	8	0.2	No Hit
CCTGCGGCAAAATAGCTCGATGCCAGAATAGATCGGAAGAGCACACGTCT	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACTC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGACCAGGCTTCATTCCCCAGATCGGAAGAGCACACGTCTGAACTCCA	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGT	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACTCC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCAGGATAGCTCAGTTGGTAGAGCAGAGGACTGAGATCGGAAGAGCACA	7	0.17500000000000002	No Hit
GAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGT	7	0.17500000000000002	TruSeq Adapter, Index 13 (97% over 36bp)
AGGGATGTAGCGCAGCTTGGTAGCGCTTTTGTTTTAGATCGGAAGAGCAC	7	0.17500000000000002	No Hit
TATTCTGGTGTCCTAGGCGTAGAGGAACCACAAGATCGGAAGAGCACACG	7	0.17500000000000002	No Hit
TGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGT	6	0.15	No Hit
CATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAATTCATGATCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACAC	5	0.125	No Hit
GGTAGTTCGACCGTGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TATTCTGGTGTCCTAGGCGTAGAGGAACCACACCAAAGATCGGAAGAGCA	5	0.125	No Hit
TCAAAAGAGGAAAGGCTTGCGGTGGATACCTAGGCACCCAGAGATCGGAA	5	0.125	No Hit
ATATCGGGTAGGTTGTGGTATTTCATTGCAGATCGGAAGAGCACACGTCT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATGCAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGA	5	0.125	No Hit
TGCAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGC	5	0.125	No Hit
TAAGTAGTAAGCCCACCCCAAGATGAGTGCTCTCTCAGATCGGAAGAGCA	5	0.125	No Hit
GACACGACTCTCGGCAACGGATATCTCGGCAGATCGGAAGAGCACACGTC	5	0.125	No Hit
AAAGGCTGACGCGGGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTTGGATTGAAGGGAGCTCTGAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTTGTAGTCTAGCTGGTCAGGATACTTAGATCGGAAGAGCACACGTC	5	0.125	No Hit
TCCTGCGGCAAAATAGCTCGATGCCAGAATAGATCGGAAGAGCACACGTC	5	0.125	No Hit
GGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGAGATCGGAAGAGCA	5	0.125	No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTAGATCGGAAGAGCACA	5	0.125	No Hit
AGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACCTGGTTGATCCTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGATTGTAGTTCAATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTA	5	0.125	TruSeq Adapter, Index 4 (97% over 35bp)
ACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTGTAGATCGGAAGAGCA	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.025	0.0	0.0	0.0	0.0
2	0.075	0.0	0.0	0.0	0.0
3	0.125	0.0	0.0	0.0	0.0
4	0.125	0.0	0.0	0.0	0.0
5	0.15	0.0	0.0	0.0	0.0
6	0.2	0.0	0.0	0.0	0.0
7	0.25	0.0	0.0	0.0	0.0
8	0.45	0.0	0.0	0.0	0.0
9	0.775	0.0	0.0	0.0	0.0
10	1.0	0.0	0.0	0.0	0.0
11	1.55	0.0	0.0	0.0	0.0
12	1.925	0.0	0.0	0.0	0.0
13	2.925	0.0	0.0	0.0	0.0
14	5.1	0.0	0.0	0.0	0.0
15	6.75	0.0	0.0	0.0	0.0
16	8.25	0.0	0.0	0.0	0.0
17	11.45	0.0	0.0	0.0	0.0
18	14.475	0.0	0.0	0.0	0.0
19	16.925	0.0	0.0	0.0	0.0
20	20.375	0.0	0.0	0.0	0.0
21	23.6	0.0	0.0	0.0	0.0
22	28.725	0.0	0.0	0.0	0.0
23	37.425	0.0	0.0	0.0	0.0
24	41.175	0.0	0.0	0.0	0.0
25	49.425	0.0	0.0	0.0	0.0
26	54.2	0.0	0.0	0.0	0.0
27	57.175	0.0	0.0	0.0	0.0
28	60.575	0.0	0.0	0.0	0.0
29	62.8	0.0	0.0	0.0	0.0
30	65.525	0.0	0.0	0.0	0.0
31	70.45	0.0	0.0	0.0	0.0
32	72.85	0.0	0.0	0.0	0.0
33	74.875	0.0	0.0	0.0	0.0
34	77.925	0.0	0.0	0.0	0.0
35	80.625	0.0	0.0	0.0	0.0
36	84.725	0.0	0.0	0.0	0.0
37	87.9	0.0	0.0	0.0	0.0
38	89.2	0.0	0.0	0.0	0.0
39	90.2	0.0	0.0	0.0	0.0
40	91.0	0.0	0.0	0.0	0.0
41	91.55	0.0	0.0	0.0	0.0
42	92.275	0.0	0.0	0.0	0.0
43	92.8	0.0	0.0	0.0	0.0
44	93.125	0.0	0.0	0.0	0.0
45	93.35	0.0	0.0	0.0	0.0
46	93.6	0.0	0.0	0.0	0.0
47	93.725	0.0	0.0	0.0	0.0
48	93.775	0.0	0.0	0.0	0.0
49	93.85	0.0	0.0	0.0	0.0
50	93.875	0.0	0.0	0.0	0.0
51	93.875	0.0	0.0	0.0	0.0
52	93.9	0.0	0.0	0.0	0.0
53	93.9	0.0	0.0	0.0	0.0
54	93.9	0.0	0.0	0.0	0.0
55	93.9	0.0	0.0	0.0	0.0
56	93.9	0.0	0.0	0.0	0.0
57	93.9	0.0	0.0	0.0	0.0
58	93.9	0.0	0.0	0.0	0.0
59	93.9	0.0	0.0	0.0	0.0
60	93.9	0.0	0.0	0.0	0.0
61	93.9	0.0	0.0	0.0	0.0
62	93.9	0.0	0.0	0.0	0.0
63	93.9	0.0	0.0	0.0	0.0
64	93.9	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATGTA	15	0.0020658788	70.00001	3
TTCAATT	15	0.0020658788	70.00001	11
ATTGGTC	15	0.0020658788	70.00001	15
CACCGCC	15	0.0020658788	70.00001	26
GATGTAG	15	0.0020658788	70.00001	4
GGATTGT	15	0.0020658788	70.00001	2
CAATTGG	15	0.0020658788	70.00001	13
TCAATTG	15	0.0020658788	70.00001	12
GTTCAAT	15	0.0020658788	70.00001	10
GATTGTA	15	0.0020658788	70.00001	3
TAGTTCA	15	0.0020658788	70.00001	8
CAGAGCA	15	0.0020658788	70.00001	21
GAGCACC	15	0.0020658788	70.00001	23
ATTGTAG	15	0.0020658788	70.00001	4
TCAGAGC	15	0.0020658788	70.00001	20
TGTAGTT	15	0.0020658788	70.00001	6
GGGATTG	15	0.0020658788	70.00001	1
GGGATGT	15	0.0020658788	70.00001	2
AGCACCG	15	0.0020658788	70.00001	24
GCACCGC	15	0.0020658788	70.00001	25
>>END_MODULE
Rejected 305493 READS because READLEN < 1
Read 305493 spots for SRR11699302.sra
Written 305493 spots for SRR11699302.sra
Rejected 305493 READS because READLEN < 1
Read 305493 spots for SRR11699302.sra
Written 305493 spots for SRR11699302.sra
Rejected 305493 READS because READLEN < 1
Read 305493 spots for SRR11699302.sra
Written 305493 spots for SRR11699302.sra
Rejected 305493 READS because READLEN < 1
Read 305493 spots for SRR11699302.sra
Written 305493 spots for SRR11699302.sra
Rejected 305493 READS because READLEN < 1
Read 305493 spots for SRR11699302.sra
Written 305493 spots for SRR11699302.sra
Rejected 305493 READS because READLEN < 1
Read 305493 spots for SRR11699302.sra
Written 305493 spots for SRR11699302.sra
Rejected 305493 READS because READLEN < 1
Read 305493 spots for SRR11699302.sra
Written 305493 spots for SRR11699302.sra
Rejected 305493 READS because READLEN < 1
Read 305493 spots for SRR11699302.sra
Written 305493 spots for SRR11699302.sra
Rejected 305493 READS because READLEN < 1
Read 305493 spots for SRR11699302.sra
Written 305493 spots for SRR11699302.sra
Rejected 305493 READS because READLEN < 1
Read 305493 spots for SRR11699302.sra
Written 305493 spots for SRR11699302.sra
Rejected 305493 READS because READLEN < 1
Read 305493 spots for SRR11699302.sra
Written 305493 spots for SRR11699302.sra
Rejected 305493 READS because READLEN < 1
Read 305493 spots for SRR11699302.sra
Written 305493 spots for SRR11699302.sra
Rejected 305496 READS because READLEN < 1
Read 305496 spots for SRR11699302.sra
Written 305496 spots for SRR11699302.sra
Rejected 305493 READS because READLEN < 1
Read 305493 spots for SRR11699302.sra
Written 305493 spots for SRR11699302.sra
Rejected 305493 READS because READLEN < 1
Read 305493 spots for SRR11699302.sra
Written 305493 spots for SRR11699302.sra
Rejected 305493 READS because READLEN < 1
Read 305493 spots for SRR11699302.sra
Written 305493 spots for SRR11699302.sra
Rejected 305493 READS because READLEN < 1
Read 305493 spots for SRR11699302.sra
Written 305493 spots for SRR11699302.sra
Rejected 305493 READS because READLEN < 1
Read 305493 spots for SRR11699302.sra
Written 305493 spots for SRR11699302.sra
Rejected 305493 READS because READLEN < 1
Read 305493 spots for SRR11699302.sra
Written 305493 spots for SRR11699302.sra
Rejected 305493 READS because READLEN < 1
Read 305493 spots for SRR11699302.sra
Written 305493 spots for SRR11699302.sra
SRR ids: ['SRR11699302.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_sttwrqbt
SRR11699302.sra spots: 6109863
blocks: [[1, 305493], [305494, 610986], [610987, 916479], [916480, 1221972], [1221973, 1527465], [1527466, 1832958], [1832959, 2138451], [2138452, 2443944], [2443945, 2749437], [2749438, 3054930], [3054931, 3360423], [3360424, 3665916], [3665917, 3971409], [3971410, 4276902], [4276903, 4582395], [4582396, 4887888], [4887889, 5193381], [5193382, 5498874], [5498875, 5804367], [5804368, 6109863]]
SRR11699302 file size 1161330
SRR11699302 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR11699302 SRR11699302_1.fastq
Input file:	SRR11699302_1.fastq
trimmed:	SRR11699302-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	20
Wed Jun 18 11:15:07 2025 >> started

Wed Jun 18 11:15:09 2025 >> done (2.525s)
6109863 reads processed; of these:
1045779 (17.12%) short reads filtered out after trimming by size control
   4312 ( 0.07%) empty reads filtered out after trimming by size control
5059772 (82.81%) reads available; of these:
4995030 (98.72%) trimmed reads available after processing
  64742 ( 1.28%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	 149902	  2.96%
 19	 236674	  4.68%
 20	 245877	  4.86%
 21	 359785	  7.11%
 22	 555785	 10.98%
 23	 243270	  4.81%
 24	 528602	 10.45%
 25	 282389	  5.58%
 26	 188611	  3.73%
 27	 218489	  4.32%
 28	 158384	  3.13%
 29	 164332	  3.25%
 30	 278121	  5.50%
 31	 154976	  3.06%
 32	 137513	  2.72%
 33	 160822	  3.18%
 34	 158373	  3.13%
 35	 256883	  5.08%
 36	 181908	  3.60%
 37	  86617	  1.71%
 38	  57646	  1.14%
 39	  50878	  1.01%
 40	  37166	  0.73%
 41	  43770	  0.87%
 42	  26264	  0.52%
 43	  19946	  0.39%
 44	  12044	  0.24%
 45	      0	  0.00%
 46	      0	  0.00%
 47	      0	  0.00%
 48	      0	  0.00%
 49	      0	  0.00%
 50	      0	  0.00%
 51	      0	  0.00%
 52	      0	  0.00%
 53	      0	  0.00%
 54	      0	  0.00%
 55	      0	  0.00%
 56	      0	  0.00%
 57	      0	  0.00%
 58	      0	  0.00%
 59	      0	  0.00%
 60	      0	  0.00%
 61	      0	  0.00%
 62	      0	  0.00%
 63	      0	  0.00%
 64	      0	  0.00%
 65	      0	  0.00%
 66	      0	  0.00%
 67	      0	  0.00%
 68	      0	  0.00%
 69	      0	  0.00%
 70	      0	  0.00%
 71	      0	  0.00%
 72	      2	  0.00%
 73	      0	  0.00%
 74	      0	  0.00%
 75	      1	  0.00%
 76	  64742	  1.28%
5059772 reads passed initial QC


criterion=sequence-density
sequence-density=4.70
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=18
prefix-density=0.01
prefix-fanout=1.0
sequence=TCCGTTGTAGTCTAGCTGGTCAGGATA


criterion=fanout-score
sequence-density=0.22
sequence-density-rank=24
fanout-score=20.37
fanout-score-rank=1
prefix-density=3.16
prefix-fanout=1.4
sequence=GTCAGGATAGCTCAGTTGGTAGAGCAGAGGACTG
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	Jun 18 11:15:32
                             Started mapping on |	Jun 18 11:15:32
                                    Finished on |	Jun 18 11:15:46
       Mapping speed, Million of reads per hour |	1301.08

                          Number of input reads |	5059772
                      Average input read length |	27
                                    UNIQUE READS:
                   Uniquely mapped reads number |	487483
                        Uniquely mapped reads % |	9.63%
                          Average mapped length |	24.72
                       Number of splices: Total |	11297
            Number of splices: Annotated (sjdb) |	5320
                       Number of splices: GT/AG |	10440
                       Number of splices: GC/AG |	608
                       Number of splices: AT/AC |	4
               Number of splices: Non-canonical |	245
                      Mismatch rate per base, % |	1.16%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.54
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.38
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	642642
             % of reads mapped to multiple loci |	12.70%
        Number of reads mapped to too many loci |	1168962
             % of reads mapped to too many loci |	23.10%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	5.20%
                     % of reads unmapped: other |	49.36%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	3929647	3929647	3929647
N_multimapping	642642	642642	642642
N_noFeature	387423	403678	467770
N_ambiguous	3524	44	29
UnstrandedReadsAssigned:96536 PositiveStrandReadsAssigned:83761 NegativeStrandReadsAssigned:19684
Dataset is classified unstranded
MeadianReadLen=25 20thPercentileLength=22 echo kmer=19
SRR11699302 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 72,539
[index] number of k-mers: 56,563,136
[index] number of equivalence classes: 414,827
[quant] running in single-end mode
[quant] will process file 1: SRR11699302-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 5,059,772 reads, 1,169,680 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 911 rounds

  72540 SRR11699302.ke.tsv
  44303 SRR11699302.se.tsv
 116843 total
==> SRR11699302.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Zm00001eb096110_T001	2067	1968	1.26626	0.605207
Zm00001eb321680_T001	3417	3318	5	1.41742
Zm00001eb323640_T001	3273	3174	0	0
Zm00001eb314140_T001	893	794	0	0
Zm00001eb080260_T001	3529	3430	0	0
Zm00001eb026490_T001	2433	2334	0	0
Zm00001eb026490_T002	2229	2130	0	0
Zm00001eb026490_T003	2220	2121	0	0
Zm00001eb281360_T001	2412	2313	0	0

==> SRR11699302.se.tsv <==
Zm00001eb015280	2
Zm00001eb000610	0
Zm00001eb033210	0
Zm00001eb044610	0
Zm00001eb014360	0
Zm00001eb051320	1
Zm00001eb021860	0
Zm00001eb011690	0
Zm00001eb016840	0
Zm00001eb052060	0
SRR11699302 completed mapping pipeline successfully
