Starting /dee2/code/volunteer_pipeline.sh SRR11699304
    current disk space = 2467438854144
    free memory = 438466464 
SRR11699304 SRAfilesize
186eca09007a77c7e42c94168a0fc702  SRR11699304.sra
SRR11699304.sra file validated
SRR11699304 is single end
SRR11699304 is conventional basespace
SRR11699304 read1 length is 76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR11699304_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22825	32.0	32.0	32.0	32.0	32.0
2	31.336	32.0	32.0	32.0	32.0	32.0
3	31.46725	32.0	32.0	32.0	32.0	32.0
4	31.4725	32.0	32.0	32.0	32.0	32.0
5	31.57025	32.0	32.0	32.0	32.0	32.0
6	35.052	36.0	36.0	36.0	36.0	36.0
7	35.03375	36.0	36.0	36.0	36.0	36.0
8	35.084	36.0	36.0	36.0	36.0	36.0
9	35.09875	36.0	36.0	36.0	36.0	36.0
10-11	35.10825	36.0	36.0	36.0	36.0	36.0
12-13	35.126000000000005	36.0	36.0	36.0	36.0	36.0
14-15	35.06425	36.0	36.0	36.0	36.0	36.0
16-17	35.103875	36.0	36.0	36.0	36.0	36.0
18-19	35.197625	36.0	36.0	36.0	36.0	36.0
20-21	35.01475	36.0	36.0	36.0	36.0	36.0
22-23	35.086375000000004	36.0	36.0	36.0	36.0	36.0
24-25	34.977875	36.0	36.0	36.0	36.0	36.0
26-27	34.924125000000004	36.0	36.0	36.0	36.0	36.0
28-29	34.933375	36.0	36.0	36.0	36.0	36.0
30-31	34.933875	36.0	36.0	36.0	36.0	36.0
32-33	34.94375	36.0	36.0	36.0	36.0	36.0
34-35	34.891000000000005	36.0	36.0	36.0	36.0	36.0
36-37	34.90275	36.0	36.0	36.0	36.0	36.0
38-39	34.848	36.0	36.0	36.0	36.0	36.0
40-41	34.760999999999996	36.0	36.0	36.0	36.0	36.0
42-43	34.914249999999996	36.0	36.0	36.0	36.0	36.0
44-45	34.816874999999996	36.0	36.0	36.0	34.0	36.0
46-47	34.813125	36.0	36.0	36.0	32.0	36.0
48-49	34.63475	36.0	36.0	36.0	32.0	36.0
50-51	34.20325	36.0	36.0	36.0	32.0	36.0
52-53	33.868875	36.0	36.0	36.0	32.0	36.0
54-55	33.524	36.0	36.0	36.0	23.0	36.0
56-57	32.8955	36.0	36.0	36.0	17.5	36.0
58-59	32.166624999999996	36.0	32.0	36.0	14.0	36.0
60-61	31.983874999999998	36.0	32.0	36.0	14.0	36.0
62-63	31.69925	36.0	32.0	36.0	14.0	36.0
64-65	31.58025	36.0	32.0	36.0	14.0	36.0
66-67	30.603749999999998	36.0	27.0	36.0	14.0	36.0
68-69	30.802625	36.0	29.5	36.0	14.0	36.0
70-71	31.394	36.0	32.0	36.0	14.0	36.0
72-73	31.627875000000003	36.0	32.0	36.0	14.0	36.0
74-75	32.123875	36.0	32.0	36.0	14.0	36.0
76	27.814	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	0.0
21	2.0
22	4.0
23	5.0
24	9.0
25	17.0
26	37.0
27	71.0
28	88.0
29	98.0
30	142.0
31	219.0
32	332.0
33	489.0
34	1057.0
35	1429.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.225	31.624999999999996	23.175	21.975
2	23.825	29.049999999999997	24.025	23.1
3	24.7	32.6	17.275	25.424999999999997
4	31.724999999999998	29.15	18.3	20.825
5	22.15	27.800000000000004	24.349999999999998	25.7
6	24.95	27.525	26.025	21.5
7	31.075000000000003	34.175	18.125	16.625
8	22.7	25.324999999999996	32.300000000000004	19.675
9	25.374999999999996	34.375	18.4	21.85
10-11	30.2	27.575	23.9875	18.2375
12-13	21.6125	22.9875	26.450000000000003	28.95
14-15	31.1875	37.25	17.4875	14.075
16-17	21.575	26.525	31.924999999999997	19.975
18-19	37.8	25.674999999999997	21.224999999999998	15.299999999999999
20-21	24.2375	34.8	23.7	17.2625
22-23	34.6625	37.45	12.4375	15.45
24-25	29.037499999999998	42.0125	12.812499999999998	16.1375
26-27	30.125	33.900000000000006	15.575	20.4
28-29	31.4375	29.225	15.049999999999999	24.2875
30-31	33.725	35.5375	8.9125	21.825
32-33	26.974999999999998	38.3875	11.375	23.2625
34-35	30.65	29.2875	15.162500000000001	24.9
36-37	24.349999999999998	33.037499999999994	12.3625	30.25
38-39	22.0125	32.975	15.1125	29.9
40-41	20.575	28.812500000000004	17.5875	33.025
42-43	24.712500000000002	23.8375	22.8875	28.5625
44-45	17.2625	31.525	19.2375	31.974999999999998
46-47	20.225	29.599999999999998	19.425	30.75
48-49	12.3	28.575	23.3	35.825
50-51	14.05	25.137500000000003	27.237499999999997	33.575
52-53	14.75	25.7	30.9875	28.5625
54-55	17.349999999999998	23.425	33.025	26.200000000000003
56-57	10.6	24.0	37.8375	27.5625
58-59	14.3625	22.975	38.05	24.6125
60-61	13.737499999999999	21.337500000000002	43.375	21.55
62-63	18.0125	19.0	41.125	21.8625
64-65	12.475	19.325	40.75	27.450000000000003
66-67	17.5125	12.837499999999999	40.375	29.275000000000002
68-69	17.599999999999998	13.5875	39.875	28.9375
70-71	18.6625	13.5125	42.9875	24.837500000000002
72-73	19.925	12.1625	40.0375	27.875
74-75	21.425	8.6875	38.6	31.2875
76	15.5	9.975000000000001	44.725	29.799999999999997
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.5
35	2.0
36	4.0
37	5.0
38	9.5
39	19.5
40	38.0
41	83.5
42	116.0
43	160.5
44	338.0
45	743.0
46	1015.0
47	769.5
48	536.0
49	480.0
50	412.0
51	340.5
52	205.5
53	111.5
54	81.0
55	82.0
56	48.5
57	11.5
58	9.0
59	7.5
60	4.0
61	1.0
62	0.0
63	0.0
64	0.5
65	0.5
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.5662368112544	57.3
2	5.041031652989449	6.45
3	1.9148104728409536	3.675
4	0.5861664712778429	1.5
5	0.5861664712778429	1.875
6	0.3907776475185619	1.5
7	0.3516998827667058	1.575
8	0.19538882375928096	1.0
9	0.19538882375928096	1.125
>10	1.016021883548261	15.8
>50	0.11723329425556857	3.9
>100	0.039077764751856196	4.3
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCC	172	4.3	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	53	1.325	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAATTCATGATCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	52	1.3	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCA	51	1.275	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAAC	50	1.25	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAT	43	1.075	TruSeq Adapter, Index 9 (97% over 36bp)
AGGGCTATAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAT	40	1.0	TruSeq Adapter, Index 9 (97% over 36bp)
AACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATG	39	0.975	TruSeq Adapter, Index 9 (97% over 36bp)
AACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	38	0.95	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	34	0.8500000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACT	30	0.75	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTGAGATCGGAAGAGCAC	29	0.7250000000000001	No Hit
AACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAG	29	0.7250000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGT	28	0.7000000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCGTTGTAGTCTAGCTGGTCAGGATACTCAGATCGGAAGAGCACACGTC	28	0.7000000000000001	No Hit
TAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCA	27	0.675	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	24	0.6	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCCTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACT	22	0.5499999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCTAGATCGGAAGAGCA	20	0.5	No Hit
CCAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT	20	0.5	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGTTAT	20	0.5	TruSeq Adapter, Index 9 (97% over 36bp)
AAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	16	0.4	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGATGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA	14	0.35000000000000003	TruSeq Adapter, Index 7 (97% over 35bp)
GATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGTTA	14	0.35000000000000003	TruSeq Adapter, Index 9 (97% over 36bp)
ACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT	13	0.325	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGACCCCAGGTCAGTCGGGACTAGATCGGAAGAGCACACGTCTGAACTC	12	0.3	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACT	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGCCATGGTGAAATTGGTAGACACGCTGCTCTTAGATCGGAAGAGCAC	11	0.27499999999999997	No Hit
CAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGT	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGACACGACTCTCGGCAACGAGATCGGAAGAGCACACGTCTGAACTCCAG	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTGAGATCGGAAGAGCAC	9	0.22499999999999998	No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCAGATCGGAAGAGCACAC	9	0.22499999999999998	No Hit
TCCTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTC	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACAC	8	0.2	No Hit
CGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA	8	0.2	TruSeq Adapter, Index 7 (97% over 35bp)
GGGATTGTAGTTCAATCGGAAGAGCACACGTCTGAACTCCAGTCACTATG	8	0.2	TruSeq Adapter, Index 10 (97% over 35bp)
CTAACGAACGAACGATTTGAAGATCGGAAGAGCACACGTCTGAACTCCAG	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAT	8	0.2	TruSeq Adapter, Index 9 (97% over 36bp)
TCGCACGGATTCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA	7	0.17500000000000002	TruSeq Adapter, Index 7 (97% over 35bp)
CGAGCACCTCTGAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGT	7	0.17500000000000002	No Hit
CGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGTT	7	0.17500000000000002	TruSeq Adapter, Index 9 (97% over 36bp)
GACACGACTCTCGGCAACGGATATCTCGGCAGATCGGAAGAGCACACGTC	7	0.17500000000000002	No Hit
TTTGGATTGAAGGGAGCTCTGAGATCGGAAGAGCACACGTCTGAACTCCA	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGACGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA	7	0.17500000000000002	TruSeq Adapter, Index 7 (97% over 35bp)
GGCGGATGTAGCCAAGTGGATCAAGGCAGTGGATTGAGATCGGAAGAGCA	7	0.17500000000000002	No Hit
GAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAT	7	0.17500000000000002	TruSeq Adapter, Index 9 (97% over 36bp)
TCCGTTGTAGTCTAGGTGGTTAGGATACTCAGATCGGAAGAGCACACGTC	6	0.15	No Hit
GCGGCAAAATAGCTCGATGCCAGAATAGATCGGAAGAGCACACGTCTGAA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCACGGATTCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATG	6	0.15	TruSeq Adapter, Index 9 (97% over 36bp)
CTAACGAACGAACGATTTGAATAGATCGGAAGAGCACACGTCTGAACTCC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACACGACTCTCGGCAACAGATCGGAAGAGCACACGTCTGAACTCCAGTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACTCC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAGATCGGAAGAGCACACGTCTGAA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGTTGTAGTCTAGTTGGTCAGGATACTCAGATCGGAAGAGCACACGTC	6	0.15	No Hit
CGACACGACTCTCGGCAACGGAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGCCCCGCGTCGCACGGATTCGTAGATCGGAAGAGCACACGTCTGAACT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAAAAGGCTGACGCGGGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGT	5	0.125	TruSeq Adapter, Index 9 (97% over 36bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCAGATCGGAAGAGCACACGTC	5	0.125	No Hit
GCGAGCGTAGTTCAGTGGTAAAACATCTCCTTGCCAAGGAGAGATCGGAA	5	0.125	No Hit
TTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGTTATC	5	0.125	TruSeq Adapter, Index 9 (97% over 36bp)
TCTCATGGAGAGTTCGATCCTGGCTAGATCGGAAGAGCACACGTCTGAAC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCTGCGGCAAAATAGCTCGATGCCAGAATAGATCGGAAGAGCACACGTC	5	0.125	No Hit
CCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAATTCATGATCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGACCCCAGGTCAGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAG	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCAC	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.05	0.0	0.0	0.0	0.0
2	0.1	0.0	0.0	0.0	0.0
3	0.2	0.0	0.0	0.0	0.0
4	0.225	0.0	0.0	0.0	0.0
5	0.25	0.0	0.0	0.0	0.0
6	0.35	0.0	0.0	0.0	0.0
7	0.475	0.0	0.0	0.0	0.0
8	0.7	0.0	0.0	0.0	0.0
9	1.65	0.0	0.0	0.0	0.0
10	2.575	0.0	0.0	0.0	0.0
11	3.325	0.0	0.0	0.0	0.0
12	4.425	0.0	0.0	0.0	0.0
13	6.75	0.0	0.0	0.0	0.0
14	11.05	0.0	0.0	0.0	0.0
15	13.4	0.0	0.0	0.0	0.0
16	15.6	0.0	0.0	0.0	0.0
17	21.55	0.0	0.0	0.0	0.0
18	25.875	0.0	0.0	0.0	0.0
19	28.55	0.0	0.0	0.0	0.0
20	32.375	0.0	0.0	0.0	0.0
21	36.55	0.0	0.0	0.0	0.0
22	42.5	0.0	0.0	0.0	0.0
23	51.625	0.0	0.0	0.0	0.0
24	55.0	0.0	0.0	0.0	0.0
25	64.025	0.0	0.0	0.0	0.0
26	68.125	0.0	0.0	0.0	0.0
27	70.625	0.0	0.0	0.0	0.0
28	73.5	0.0	0.0	0.0	0.0
29	75.45	0.0	0.0	0.0	0.0
30	77.6	0.0	0.0	0.0	0.0
31	80.8	0.0	0.0	0.0	0.0
32	82.4	0.0	0.0	0.0	0.0
33	83.625	0.0	0.0	0.0	0.0
34	85.625	0.0	0.0	0.0	0.0
35	86.975	0.0	0.0	0.0	0.0
36	89.875	0.0	0.0	0.0	0.0
37	91.975	0.0	0.0	0.0	0.0
38	92.925	0.0	0.0	0.0	0.0
39	93.275	0.0	0.0	0.0	0.0
40	93.65	0.0	0.0	0.0	0.0
41	93.925	0.0	0.0	0.0	0.0
42	94.325	0.0	0.0	0.0	0.0
43	94.5	0.0	0.0	0.0	0.0
44	94.575	0.0	0.0	0.0	0.0
45	94.75	0.0	0.0	0.0	0.0
46	94.875	0.0	0.0	0.0	0.0
47	94.875	0.0	0.0	0.0	0.0
48	94.875	0.0	0.0	0.0	0.0
49	94.95	0.0	0.0	0.0	0.0
50	94.95	0.0	0.0	0.0	0.0
51	95.025	0.0	0.0	0.0	0.0
52	95.025	0.0	0.0	0.0	0.0
53	95.025	0.0	0.0	0.0	0.0
54	95.025	0.0	0.0	0.0	0.0
55	95.025	0.0	0.0	0.0	0.0
56	95.025	0.0	0.0	0.0	0.0
57	95.05	0.0	0.0	0.0	0.0
58	95.05	0.0	0.0	0.0	0.0
59	95.05	0.0	0.0	0.0	0.0
60	95.05	0.0	0.0	0.0	0.0
61	95.05	0.0	0.0	0.0	0.0
62	95.05	0.0	0.0	0.0	0.0
63	95.05	0.0	0.0	0.0	0.0
64	95.05	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCATGA	15	0.0020658788	70.00001	4
TCTAGAT	15	0.0020658788	70.00001	34
TTGTAGT	15	0.0020658788	70.00001	5
TCATGAT	15	0.0020658788	70.00001	5
TGTAGCT	15	0.0020658788	70.00001	6
CTGCTTT	15	0.0020658788	70.00001	70
ATGTAGC	15	0.0020658788	70.00001	5
GCTCTAG	15	0.0020658788	70.00001	32
ATTCATG	15	0.0020658788	70.00001	3
GTAGCTC	15	0.0020658788	70.00001	7
TGGTCAG	20	0.0064587677	52.5	17
TAGCTCA	20	0.0064587677	52.5	8
CTCTAGA	20	0.0064587677	52.5	33
TCTGCTT	75	4.3394008E-5	28.0	70
TTCTGCT	80	6.740297E-5	26.25	69
CTTCTGC	80	6.740297E-5	26.25	68
TCTTCTG	90	1.5026014E-4	23.333334	67
GTCTTCT	110	2.0955404E-5	22.272726	66
CGTCTTC	125	5.6304074E-5	19.6	65
CCGTCTT	150	2.2864617E-4	16.333334	64
>>END_MODULE
Rejected 370313 READS because READLEN < 1
Read 370313 spots for SRR11699304.sra
Written 370313 spots for SRR11699304.sra
Rejected 370313 READS because READLEN < 1
Read 370313 spots for SRR11699304.sra
Written 370313 spots for SRR11699304.sra
Rejected 370313 READS because READLEN < 1
Read 370313 spots for SRR11699304.sra
Written 370313 spots for SRR11699304.sra
Rejected 370313 READS because READLEN < 1
Read 370313 spots for SRR11699304.sra
Written 370313 spots for SRR11699304.sra
Rejected 370313 READS because READLEN < 1
Read 370313 spots for SRR11699304.sra
Written 370313 spots for SRR11699304.sra
Rejected 370313 READS because READLEN < 1
Read 370313 spots for SRR11699304.sra
Written 370313 spots for SRR11699304.sra
Rejected 370313 READS because READLEN < 1
Read 370313 spots for SRR11699304.sra
Written 370313 spots for SRR11699304.sra
Rejected 370313 READS because READLEN < 1
Read 370313 spots for SRR11699304.sra
Written 370313 spots for SRR11699304.sra
Rejected 370313 READS because READLEN < 1
Read 370313 spots for SRR11699304.sra
Written 370313 spots for SRR11699304.sra
Rejected 370313 READS because READLEN < 1
Read 370313 spots for SRR11699304.sra
Written 370313 spots for SRR11699304.sra
Rejected 370313 READS because READLEN < 1
Read 370313 spots for SRR11699304.sra
Written 370313 spots for SRR11699304.sra
Rejected 370313 READS because READLEN < 1
Read 370313 spots for SRR11699304.sra
Written 370313 spots for SRR11699304.sra
Rejected 370313 READS because READLEN < 1
Read 370313 spots for SRR11699304.sra
Written 370313 spots for SRR11699304.sra
Rejected 370313 READS because READLEN < 1
Read 370313 spots for SRR11699304.sra
Written 370313 spots for SRR11699304.sra
Rejected 370313 READS because READLEN < 1
Read 370313 spots for SRR11699304.sra
Written 370313 spots for SRR11699304.sra
Rejected 370313 READS because READLEN < 1
Read 370313 spots for SRR11699304.sra
Written 370313 spots for SRR11699304.sra
Rejected 370329 READS because READLEN < 1
Read 370329 spots for SRR11699304.sra
Written 370329 spots for SRR11699304.sra
Rejected 370313 READS because READLEN < 1
Read 370313 spots for SRR11699304.sra
Written 370313 spots for SRR11699304.sra
Rejected 370313 READS because READLEN < 1
Read 370313 spots for SRR11699304.sra
Written 370313 spots for SRR11699304.sra
Rejected 370313 READS because READLEN < 1
Read 370313 spots for SRR11699304.sra
Written 370313 spots for SRR11699304.sra
SRR ids: ['SRR11699304.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_m9ush_iw
SRR11699304.sra spots: 7406276
blocks: [[1, 370313], [370314, 740626], [740627, 1110939], [1110940, 1481252], [1481253, 1851565], [1851566, 2221878], [2221879, 2592191], [2592192, 2962504], [2962505, 3332817], [3332818, 3703130], [3703131, 4073443], [4073444, 4443756], [4443757, 4814069], [4814070, 5184382], [5184383, 5554695], [5554696, 5925008], [5925009, 6295321], [6295322, 6665634], [6665635, 7035947], [7035948, 7406276]]
SRR11699304 file size 1408205
SRR11699304 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR11699304 SRR11699304_1.fastq
Input file:	SRR11699304_1.fastq
trimmed:	SRR11699304-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	20
Wed Jun 18 10:26:22 2025 >> started

Wed Jun 18 10:26:26 2025 >> done (3.117s)
7406276 reads processed; of these:
2129852 (28.76%) short reads filtered out after trimming by size control
   3363 ( 0.05%) empty reads filtered out after trimming by size control
5273061 (71.20%) reads available; of these:
5233703 (99.25%) trimmed reads available after processing
  39358 ( 0.75%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	 232154	  4.40%
 19	 331281	  6.28%
 20	 330006	  6.26%
 21	 482967	  9.16%
 22	 691740	 13.12%
 23	 268608	  5.09%
 24	 585999	 11.11%
 25	 292218	  5.54%
 26	 185223	  3.51%
 27	 228360	  4.33%
 28	 137410	  2.61%
 29	 146739	  2.78%
 30	 257465	  4.88%
 31	 124930	  2.37%
 32	 110959	  2.10%
 33	 134658	  2.55%
 34	 128155	  2.43%
 35	 205702	  3.90%
 36	 165372	  3.14%
 37	  58210	  1.10%
 38	  36215	  0.69%
 39	  27874	  0.53%
 40	  21014	  0.40%
 41	  23166	  0.44%
 42	  13365	  0.25%
 43	   8563	  0.16%
 44	   5349	  0.10%
 45	      0	  0.00%
 46	      0	  0.00%
 47	      0	  0.00%
 48	      0	  0.00%
 49	      0	  0.00%
 50	      0	  0.00%
 51	      0	  0.00%
 52	      0	  0.00%
 53	      0	  0.00%
 54	      0	  0.00%
 55	      0	  0.00%
 56	      0	  0.00%
 57	      0	  0.00%
 58	      0	  0.00%
 59	      0	  0.00%
 60	      0	  0.00%
 61	      0	  0.00%
 62	      0	  0.00%
 63	      0	  0.00%
 64	      0	  0.00%
 65	      0	  0.00%
 66	      0	  0.00%
 67	      0	  0.00%
 68	      0	  0.00%
 69	      0	  0.00%
 70	      0	  0.00%
 71	      0	  0.00%
 72	      0	  0.00%
 73	      0	  0.00%
 74	      1	  0.00%
 75	      0	  0.00%
 76	  39358	  0.75%
5273061 reads passed initial QC


criterion=sequence-density
sequence-density=5.43
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=14
prefix-density=0.02
prefix-fanout=1.0
sequence=TCCGTTGTAGTCTAGCTGGTCAGGATA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=46
fanout-score=107.01
fanout-score-rank=1
prefix-density=0.54
prefix-fanout=1.0
sequence=AGCCAAGTGTGGAGAGGATAACTGCTGAAAGCATATAAGTAGTAAGCCCACCCCAAGATGAGTGCTCTCTCCTCCGACTTCC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	Jun 18 10:26:37
                             Started mapping on |	Jun 18 10:26:38
                                    Finished on |	Jun 18 10:26:52
       Mapping speed, Million of reads per hour |	1355.93

                          Number of input reads |	5273061
                      Average input read length |	25
                                    UNIQUE READS:
                   Uniquely mapped reads number |	553562
                        Uniquely mapped reads % |	10.50%
                          Average mapped length |	23.29
                       Number of splices: Total |	12818
            Number of splices: Annotated (sjdb) |	5665
                       Number of splices: GT/AG |	12066
                       Number of splices: GC/AG |	577
                       Number of splices: AT/AC |	3
               Number of splices: Non-canonical |	172
                      Mismatch rate per base, % |	1.14%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.53
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.20
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	757222
             % of reads mapped to multiple loci |	14.36%
        Number of reads mapped to too many loci |	1206890
             % of reads mapped to too many loci |	22.89%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	4.55%
                     % of reads unmapped: other |	47.70%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	3962277	3962277	3962277
N_multimapping	757222	757222	757222
N_noFeature	441771	458828	532529
N_ambiguous	4055	54	38
UnstrandedReadsAssigned:107736 PositiveStrandReadsAssigned:94680 NegativeStrandReadsAssigned:20995
Dataset is classified unstranded
MeadianReadLen=24 20thPercentileLength=21 echo kmer=19
SRR11699304 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 72,539
[index] number of k-mers: 56,563,136
[index] number of equivalence classes: 414,827
[quant] running in single-end mode
[quant] will process file 1: SRR11699304-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 5,273,061 reads, 1,180,281 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 938 rounds

  72540 SRR11699304.ke.tsv
  44303 SRR11699304.se.tsv
 116843 total
==> SRR11699304.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Zm00001eb096110_T001	2067	1968	1	0.480615
Zm00001eb321680_T001	3417	3318	4	1.14027
Zm00001eb323640_T001	3273	3174	1	0.297999
Zm00001eb314140_T001	893	794	0	0
Zm00001eb080260_T001	3529	3430	0	0
Zm00001eb026490_T001	2433	2334	0	0
Zm00001eb026490_T002	2229	2130	0	0
Zm00001eb026490_T003	2220	2121	0	0
Zm00001eb281360_T001	2412	2313	1	0.408928

==> SRR11699304.se.tsv <==
Zm00001eb015280	1
Zm00001eb000610	0
Zm00001eb033210	0
Zm00001eb044610	0
Zm00001eb014360	0
Zm00001eb051320	1
Zm00001eb021860	0
Zm00001eb011690	0
Zm00001eb016840	0
Zm00001eb052060	0
SRR11699304 completed mapping pipeline successfully
