Starting /dee2/code/volunteer_pipeline.sh SRR11699470
    current disk space = 2473939947520
    free memory = 1253294620 
SRR11699470 SRAfilesize
7a978f40479c41fe8c4548b91780e191  SRR11699470.sra
SRR11699470.sra file validated
SRR11699470 is single end
SRR11699470 is conventional basespace
SRR11699470 read1 length is 86 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR11699470_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	186
Sequences flagged as poor quality	0
Sequence length	86
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.93010752688172	32.0	32.0	32.0	32.0	32.0
2	30.913978494623656	32.0	32.0	32.0	32.0	32.0
3	31.322580645161292	32.0	32.0	32.0	32.0	32.0
4	31.198924731182796	32.0	32.0	32.0	32.0	32.0
5	30.698924731182796	32.0	32.0	32.0	32.0	32.0
6	34.61827956989247	36.0	36.0	36.0	32.0	36.0
7	34.98924731182796	36.0	36.0	36.0	32.0	36.0
8	35.09139784946237	36.0	36.0	36.0	36.0	36.0
9	34.31182795698925	36.0	36.0	36.0	32.0	36.0
10-11	34.5510752688172	36.0	36.0	36.0	32.0	36.0
12-13	34.57258064516129	36.0	36.0	36.0	32.0	36.0
14-15	34.94086021505376	36.0	36.0	36.0	36.0	36.0
16-17	34.64516129032258	36.0	36.0	36.0	32.0	36.0
18-19	34.946236559139784	36.0	36.0	36.0	34.0	36.0
20-21	34.28225806451613	36.0	36.0	36.0	29.5	36.0
22-23	34.817204301075265	36.0	36.0	36.0	34.0	36.0
24-25	34.59139784946237	36.0	36.0	36.0	32.0	36.0
26-27	34.00537634408602	36.0	36.0	36.0	32.0	36.0
28-29	33.10752688172043	36.0	36.0	36.0	17.5	36.0
30-31	33.35752688172043	36.0	36.0	36.0	17.5	36.0
32-33	33.067204301075265	36.0	36.0	36.0	14.0	36.0
34-35	33.56989247311828	36.0	36.0	36.0	20.5	36.0
36-37	32.60215053763441	36.0	36.0	36.0	14.0	36.0
38-39	32.975806451612904	36.0	36.0	36.0	14.0	36.0
40-41	33.5	36.0	36.0	36.0	14.0	36.0
42-43	32.63172043010753	36.0	36.0	36.0	14.0	36.0
44-45	32.99731182795699	36.0	36.0	36.0	14.0	36.0
46-47	32.744623655913976	36.0	36.0	36.0	14.0	36.0
48-49	32.77956989247312	36.0	36.0	36.0	14.0	36.0
50-51	32.49193548387097	36.0	36.0	36.0	14.0	36.0
52-53	32.56720430107527	36.0	36.0	36.0	14.0	36.0
54-55	32.52150537634409	36.0	34.0	36.0	14.0	36.0
56-57	31.612903225806452	36.0	32.0	36.0	14.0	36.0
58-59	31.52956989247312	36.0	32.0	36.0	14.0	36.0
60-61	31.352150537634408	36.0	32.0	36.0	14.0	36.0
62-63	30.155913978494624	36.0	20.5	36.0	14.0	36.0
64-65	30.1747311827957	36.0	27.0	36.0	14.0	36.0
66-67	29.870967741935484	36.0	24.0	36.0	14.0	36.0
68-69	29.782258064516128	36.0	24.0	36.0	14.0	36.0
70-71	30.077956989247312	36.0	27.0	36.0	14.0	36.0
72-73	30.387096774193548	36.0	26.5	36.0	14.0	36.0
74-75	29.658602150537636	36.0	14.0	36.0	14.0	36.0
76-77	29.922043010752688	36.0	20.5	36.0	14.0	36.0
78-79	29.263440860215052	36.0	14.0	36.0	14.0	36.0
80-81	29.66935483870968	36.0	14.0	36.0	14.0	36.0
82-83	30.102150537634408	36.0	24.0	36.0	14.0	36.0
84-85	29.666666666666664	36.0	14.0	36.0	14.0	36.0
86	27.919354838709676	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	1.0
22	7.0
23	6.0
24	5.0
25	7.0
26	4.0
27	4.0
28	1.0
29	7.0
30	10.0
31	7.0
32	8.0
33	14.0
34	43.0
35	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.344086021505376	23.655913978494624	30.107526881720432	19.892473118279568
2	21.50537634408602	25.268817204301076	15.591397849462366	37.634408602150536
3	23.118279569892472	21.50537634408602	16.129032258064516	39.247311827956985
4	39.784946236559136	27.956989247311824	15.591397849462366	16.666666666666664
5	15.053763440860216	26.881720430107524	32.795698924731184	25.268817204301076
6	16.666666666666664	19.892473118279568	39.247311827956985	24.193548387096776
7	46.236559139784944	13.978494623655912	23.118279569892472	16.666666666666664
8	17.741935483870968	20.967741935483872	42.473118279569896	18.817204301075268
9	17.20430107526882	43.54838709677419	23.118279569892472	16.129032258064516
10-11	37.096774193548384	14.516129032258066	29.838709677419356	18.548387096774192
12-13	19.892473118279568	16.129032258064516	31.989247311827956	31.989247311827956
14-15	34.946236559139784	32.526881720430104	17.20430107526882	15.32258064516129
16-17	17.473118279569892	26.0752688172043	30.376344086021508	26.0752688172043
18-19	44.623655913978496	22.311827956989248	15.32258064516129	17.741935483870968
20-21	28.763440860215056	25.537634408602152	23.118279569892472	22.58064516129032
22-23	31.72043010752688	36.55913978494624	19.086021505376344	12.634408602150538
24-25	27.688172043010752	41.66666666666667	17.20430107526882	13.440860215053762
26-27	23.387096774193548	36.29032258064516	24.462365591397848	15.86021505376344
28-29	29.301075268817208	28.225806451612907	20.43010752688172	22.043010752688172
30-31	38.97849462365591	32.25806451612903	13.978494623655912	14.78494623655914
32-33	31.451612903225808	36.82795698924731	10.21505376344086	21.50537634408602
34-35	37.903225806451616	37.634408602150536	11.021505376344086	13.440860215053762
36-37	30.376344086021508	40.053763440860216	4.838709677419355	24.731182795698924
38-39	25.268817204301076	41.66666666666667	8.60215053763441	24.462365591397848
40-41	20.967741935483872	33.064516129032256	10.483870967741936	35.483870967741936
42-43	26.344086021505376	26.344086021505376	19.623655913978492	27.688172043010752
44-45	23.387096774193548	25.537634408602152	20.161290322580644	30.913978494623656
46-47	23.118279569892472	34.67741935483871	17.20430107526882	25.0
48-49	16.129032258064516	28.225806451612907	21.236559139784948	34.40860215053764
50-51	12.096774193548388	33.064516129032256	18.27956989247312	36.55913978494624
52-53	14.24731182795699	27.688172043010752	21.236559139784948	36.82795698924731
54-55	11.559139784946236	26.0752688172043	23.387096774193548	38.97849462365591
56-57	13.709677419354838	33.33333333333333	17.741935483870968	35.215053763440864
58-59	15.053763440860216	31.451612903225808	15.053763440860216	38.44086021505376
60-61	14.78494623655914	37.634408602150536	14.24731182795699	33.33333333333333
62-63	13.440860215053762	36.29032258064516	13.440860215053762	36.82795698924731
64-65	12.634408602150538	33.33333333333333	23.655913978494624	30.376344086021508
66-67	12.634408602150538	24.193548387096776	27.688172043010752	35.483870967741936
68-69	17.20430107526882	23.118279569892472	28.49462365591398	31.182795698924732
70-71	13.172043010752688	21.774193548387096	34.946236559139784	30.107526881720432
72-73	27.688172043010752	13.172043010752688	30.376344086021508	28.763440860215056
74-75	19.623655913978492	16.93548387096774	26.881720430107524	36.55913978494624
76-77	25.0	9.13978494623656	38.17204301075269	27.688172043010752
78-79	12.096774193548388	11.827956989247312	37.096774193548384	38.97849462365591
80-81	17.741935483870968	8.870967741935484	40.59139784946236	32.795698924731184
82-83	18.010752688172044	12.903225806451612	41.935483870967744	27.1505376344086
84-85	15.32258064516129	18.010752688172044	40.053763440860216	26.61290322580645
86	19.35483870967742	20.43010752688172	44.086021505376344	16.129032258064516
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.5
31	0.5
32	0.5
33	0.5
34	0.0
35	0.5
36	1.0
37	1.0
38	1.0
39	1.5
40	1.5
41	0.5
42	2.5
43	5.0
44	5.0
45	8.0
46	13.0
47	14.0
48	17.0
49	21.5
50	22.0
51	22.5
52	25.0
53	18.0
54	10.5
55	9.0
56	4.0
57	2.0
58	2.5
59	2.0
60	1.0
61	1.0
62	1.0
63	0.5
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	186.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.78494623655914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.40718562874252	86.55913978494624
2	2.9940119760479043	5.376344086021505
3	0.0	0.0
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.5988023952095809	8.064516129032258
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	15	8.064516129032258	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACACGACTCTCGGCAACGGATATCTCGAGATCGGAAGAGCACACGTCTG	2	1.0752688172043012	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACTC	2	1.0752688172043012	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGACCCCAGGTCAGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAG	2	1.0752688172043012	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGACCCCAGGTCAGTCGGGACTAGATCGGAAGAGCACACGTCTGAACTC	2	1.0752688172043012	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGTCTGTAGTCCAACGGTTAGGATAAGATCGGAAGAGCACACGTCTGAA	2	1.0752688172043012	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACACGACTCTCGGCAACGGATATCTCGGCTCTCAGATCGTAAGAGCAA	1	0.5376344086021506	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAAGAACCTAAGAGCAGACGTCTTC	1	0.5376344086021506	No Hit
AACAATTTGTTGAACAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG	1	0.5376344086021506	TruSeq Adapter, Index 15 (97% over 36bp)
CGACACGACTCTCGGCAACGGATATCTCGGCAGATCGGAAGAGCACACGT	1	0.5376344086021506	No Hit
AATCATGACTGTGCCTAGGGCCTAAGATCGGAAGAGCACACGTCTGGAAC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TGTTCTAATGTTTGTTTTGTAGAGATCGGAAGAACACACGTCTGAACTCC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTGAGATCGGAAGAGCAC	1	0.5376344086021506	No Hit
GACACGACTCTCGGCAACGGATATCAGATCGGAAGAGCACACGTCTGAAC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCTCATAGCCGAGTCGAGACAGAAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTAACGAACGAACGATTTGAACAGATCGGAAGATCACACGTCTGAACTCC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TGGCTGTGGGGAAAAGCTGGCTGTAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACGATTAGGCCTTATAACCTCAACCTCAAAATTTAAAAACTAAGTAAT	1	0.5376344086021506	No Hit
GGGGATGTAGCTCAGATCGGAAGAACACACGTCTGAACTCCAGTTACACG	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TAGGACGGCGCGGCTGCTTCGGTGAGCCGTGCCAGATCGGAAGAGCACAC	1	0.5376344086021506	No Hit
AAAACCTCAGGATAGCTAGAGCTAAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGATGATTCATGATAACTTGAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAAGTACCGCGAGGGAAAGATGAAAAGGACTTTGAAAAAAGAGATCGGAA	1	0.5376344086021506	No Hit
TAAAAGGCTGACGCGGGCTCTGCCCGCCAGATCGGAAGAGCACACGTCTG	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAACTGAATTTGGTTTCTGAGCCAAGATCGGAAGAGCACACGTCTGAAC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGCACGGTGCTTACCCCGGGCTTGTCGGATGTACTTGAAGATCGGAAGA	1	0.5376344086021506	No Hit
CGATGATTCATGATAACTTGAAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGCCCCGAACCCGTCGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAG	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GACACGACTCTCGGCAACGGATATCTCGGCTCAGATCGGAAGAGCACACG	1	0.5376344086021506	No Hit
ATCGAATTCTAAACTAAATAGCTTAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACTGAGATCCAGCCCCGCGTCGCACGGATTCGTAGATCGGAAGAGGACA	1	0.5376344086021506	No Hit
AGATAACGCAGGTGTCCTAAGATGAGCTCAACGAGAACAAGATCGGAAGA	1	0.5376344086021506	No Hit
GACACGACTCTCGGCAACGGATATCTCGAGATAGGAAGAGCACACGACTG	1	0.5376344086021506	No Hit
CGTTATTTTACTTATTCCGTGGGTCGGAAAGATCGGAAGAGCACACGTCT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGAGTCGGGTTGTTTGGGAATGCAAGATCGGAAGAGCACACGTCTGAAG	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GTATAGAAACAACTAGGCTCTCCAAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGCTGTAGTTTAGTGGCGAGAATTCCACGAGATCGGAAGAGCACACGT	1	0.5376344086021506	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTAGATCGGAAGAGCACA	1	0.5376344086021506	No Hit
TCGACACGACTCTCGGCAACGGATATCTCGAGATCGGAAGAGCACACGTC	1	0.5376344086021506	No Hit
GCACCAGTCGTCAAGACCTACAAAACTACCCAGATCGGAACACCACACGT	1	0.5376344086021506	No Hit
GCGGCCCCAGGTCAGTCGGGACTAGATCGGAAGAGCACACGTCTGAACTC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATAGAGTTCGGTCCTAATTCGATAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACATCATAGGATTCCGGTCCTATTGTGTTGGCCTTCGGGATCGGAAGATC	1	0.5376344086021506	No Hit
ACTGCATTATTTTCGGAGACGGGCAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAGAATTTGTTCGGAGACATGAGATCGGAAGAGCACACGTCTGAACTCC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGTGAACAGCCTCTGGCCAATGGAAAGATCGGAAGAGCACACGTCTGAAC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATAACCACTGGGTGATGACTGAGATCGGAAGAGCACACGTCTGAACTCC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCGGAGGTAGGGTCCAGCGGCCGGAAGAGCACCGCAAGATCGGAAGAGCA	1	0.5376344086021506	No Hit
CAAGGCTAAATACAGGCGAGAGACCGATAAGATCGGGAGAGCACACGTCT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCCGGCCCCGAACCCGTCGGCAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGAACGGTTTGATTAGATCAAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACGTATTTATGTAATCAATGGATAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGGCGGCAGACGGCGACGTTAAGATCGGAAGAGCACACGTCTGAACTC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGCCCCGTGCTCCAGATCGGAAGAGCACACGTAGGAAATCCAGTCACG	1	0.5376344086021506	No Hit
TACGTTGTAGTCTAGTTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTAGCAAATATTCAAATGAGAACTTTGAAGGAGATCGGAAGAGCACACGT	1	0.5376344086021506	No Hit
AAAAACTCTAGACGATTATTCCAGATCGGAAGAGCACACGTCTGAACTCC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGTTGTGGTCTAGCTGGTCAGGATAATAAGGGAAGAGGAGCCGTCTTC	1	0.5376344086021506	No Hit
CTACACTGATGTATCCAACGAGTATAAGATCGGAAGAGCACACGTCTGAA	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGTCCGTTGTTGCTGGCGCCAGATCGGAAGAGCACACGTCTGAACTCC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTATGACTGAACGCCTCTAAGTCAGAATCCAGATCGGAAGAGCACACGTC	1	0.5376344086021506	No Hit
TCCGTTGTGGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTAGCTCGGAAGAGCACAACTCA	1	0.5376344086021506	No Hit
AGGATTATGATGCTGATGTCGGTGAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TACCTAGAGGGAAAGATGAAAAGGAGTTTGAAAAGAGATAGGAAGAGAAC	1	0.5376344086021506	No Hit
GCGACCCCAGGTCAGTCGGGAAATCGGAAGAGCACACGTCTGAACTCCGT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
AACATTGTTTTACGATGTAGACTGAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGCTGTAGTCTAGCTGGTCAGGAAGATCGGAAGAGCACACGTCTGAAC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGTTGTAGTCTAGCTGGTGAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCACAAAGGGTGTTGGTCGATTAAGACAGCAGGACAAGAACGGAATAG	1	0.5376344086021506	No Hit
ACATTATCTCGTCTGAGCCAGACCAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCATAGTTCTCTTCGTCCCGACCGAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCATGACGAAAATCGTCTGGCTCTCTGAGGCAGATCGGAAGAGCACACG	1	0.5376344086021506	No Hit
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCGTCACACGA	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CACGACTCTCGGCAACGGATATCTCGGCAGATCGGAAGAGCACACGTCTG	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATTCTGGTGTCCTAGGCGTAGAGGAACCACAGATCGGAAGAGCACACGT	1	0.5376344086021506	No Hit
GGGATTGTAGTTCAATTGGTCAGAGAAGATCGGAAGCGCAAGCGAAAAAC	1	0.5376344086021506	No Hit
ATTCTGTTAGATGATGAAATTTTAGATCGGAGGAGCACACGTCTTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TTTAAGTTGTTGCAGTTAAAAAGCTCGTAAGATCGGAAGAGCACACGTCT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCTAAGTCTGTCAAGCTCGCTTAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAATATATCTGGAGCGCACGAGTCA	1	0.5376344086021506	No Hit
TCCGTTGTAGTCTAGCTGGCCAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCGAGGGAAAGATGAAAAGGACTTTGAAAAAAGAGATCGGAAGAGCAC	1	0.5376344086021506	No Hit
CATGTGAACGGCACTTGCACAGATCGGAAGAGCGCACGTCTGAACTCCAG	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TTTTAATGTCTGGCCTGCACGTGTAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAAAAAACGGATCGAAAACAGGTAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTTTTTGGACAGGTCGCTAGGTCAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAAGATTGTATCATGGGTTCGAATCCCACAGATCGGAAGAGCACACGTCT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGATGATTCATGATAACTTAGATCGGAAGAGCACACGTCTGAACTCCAT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AAGATGAAAAGGACTTTGAAAAGAGATCGGAAGAGCACACGTCTGAACTC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGACATGTGGGGTCCATGGTATAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGAAACAACAAAAGCTGTTCGTCTATTAAGAAGAACAGAAGACCACACC	1	0.5376344086021506	No Hit
AGACGGTCTTAGAAAAAAAGAGAAAGATCGGAAGAGCACACGTCTGAACA	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
CGACACGACTCTCGGCAACGAGATCGGAAGAGCACACGTCTGAACTCCAG	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGACACGACTCTCGGCAACGGATATCAGGATCGGAAGAGCACACGTCTGA	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TTAGTTGGTGGAGCGATTAGATCGGAAGAGCACACGTCTGAACTCCATCA	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TCCGTTGTAGTCTAGTTGGTCAGGATAGATCGGAAGAGCACACGTCTGAA	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCCGGGTCGTGGCGCCGGCGCCGTTAATTTGTAGAAATTTGAATATAGG	1	0.5376344086021506	No Hit
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTTACACG	1	0.5376344086021506	TruSeq Adapter, Index 5 (97% over 35bp)
CGACTCGACACTAGACACGGATTGAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCCAGATCGGAAGAGCA	1	0.5376344086021506	No Hit
TTCGGAATACTTCGAAGACGGTCAGATCGGAAGAGCACACGTCTGAACTC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACAGTGAGGAACTAATTTTGCTAATGTATCTATGAGATCGGAAGAGCAC	1	0.5376344086021506	No Hit
ATTGTCGAGCGGAATATAAACACCAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGCAGTGGGAGGGGAAAGTGATCTCTGAGATCGGAAGAGCACACGTCTG	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAATGGCAAATGGAGGATGTGTAGATCGGAAGAGCACACGTCTGAACTCC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCTCGGTGGGACCTCCAAGATCGGAAGAGCACACGTCTGAACTCCATCA	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TCCAGCCCCGCGTCGCACGGATTCGTAGATCGGAAGAGCACACGTCTGAT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GTCCTCGAGGATGCTGACGCAATGTGATTTCTGCCCAGTGCTCTGAAGAT	1	0.5376344086021506	No Hit
GTTCTGCACTGTATATTGCACTGTAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACTCCGTACAACGCTAAGATCGGAAGAGCAAAAGTCTGAACTCCAGACA	1	0.5376344086021506	No Hit
CTAGTCCGACTTTGTGAAATGACAAATCGGAAGAGCACACGTCTGAACTC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TCTGGAATTGCTTGAGCCTCGGAGATCGGAAGAGCACACGTCTGAACTCC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGTAGCAGGTGGGATTTGGGAGAGAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCAAATTCTGTGATTGGATTACGCATCAATCTCAGCTCAGATCGCAAGAG	1	0.5376344086021506	No Hit
ACCTCCTGGGAAGTCCTCGTGTTGCATTCCTTTTTTAAGATCGGAAGAGC	1	0.5376344086021506	No Hit
GGATAGCGACGGTGTCAGAAAAAAGATCGGAAGAGCACACGTCTGAACTC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGATCTTCGTGGTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTTAC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GCGACCCCAGGTCAGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GGGGACGTAGCTCATATGGTAGAGCGCTCGCTTCGAGATCGGAAGAGCAC	1	0.5376344086021506	No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTGATGAAGGGATGGCAC	1	0.5376344086021506	No Hit
GGCTCATTAAATCAGTTATAGTTTGTTTGATGGTACGTGCTACTCGGAAG	1	0.5376344086021506	No Hit
TCTGGCTGTGAGGAGAATCTGGTAGATCGGAAGAGCACACGTCTGAACTC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACAAGACTCTCGGCAACGGATAAGATCGGAAGGGCACACGTCTGAACC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
CAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCGTCA	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CCATATGGATTGAAGGGGATTGAGAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGAATGTAACTGAAGTAGTCAGATCGGAAGAGCACACGTCTGAACTCC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTATGCGAAAAAGACGAATATCA	1	0.5376344086021506	No Hit
ACACGACTCTCGGCAACGGATATCTCGAGATCGGAAGAGCACACGTCTGA	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCGTCCAAGGCTAAATACAGGAGATCGGAAGAGCACACGTCTGAACTC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACGCGTCTGA	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ACAAGACTCACCACAACGAATAATAACCCAAGATCACAAGTAAAAACACC	1	0.5376344086021506	No Hit
CCGACCTTAGCTCAGTTGGTAGAGCTGAGGACTGAGATCGGAAGAGCACA	1	0.5376344086021506	No Hit
CGACACGACTCTCGGCAACGGATATCTCGGAGATCGGAAGAGCACACGTC	1	0.5376344086021506	No Hit
GTGTCTGGTAATTGGAATGAGTAAGATCGGAAGAGCACACGTCTGAACTC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGGTTCAGCCGGAGGTAGGGTCCAGATCGGAAGAGCACACGTCTGAAC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACAGACTGAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCGCAC	1	0.5376344086021506	TruSeq Adapter, Index 5 (97% over 35bp)
CTCCGGTGTTTACTTTGTCCCCAGATCGGAAGAGCACACGTCTGAACTCC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGATGTCCTGTGTTGTCCAATAGATCGGAAGAGCACACGTCTGAACTCC	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCTCAATCTACTTTAATACCTGTAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTGAGATAGATAGAGAAC	1	0.5376344086021506	No Hit
TCCGTTGCAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGTATGAACTAATTCGAACTGTGAAATTGCGAATGAGATCGGAAGAGCA	1	0.5376344086021506	No Hit
GTCTATTGTTGACGGTCGTTAACTAGATCGGAAGAGCAGACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TTACCAGGACGATAAAACGCGCATCTGAGCTAGATCGGAAGAGCACACGT	1	0.5376344086021506	No Hit
AGTAATCCGACTAGGAATCAATCCAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTAGTGGGATAAAACATGTGAGATCGGAAGGGCACACGTCTGAGCTCCA	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTATAACGCCACGGCTCA	1	0.5376344086021506	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACAAGTCTGA	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCTCCGCTTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1	0.5376344086021506	No Hit
CACGACTCTCGGCAACGGATATCTCGAGATCGGAAGAGCACACGTCTGAA	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTACAGACCCGGGTTCGTTTCCCGGCTGGTGCACAAAGATCGGAAGAGC	1	0.5376344086021506	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAGGAGCGGAGGGGACCCCTGATGA	1	0.5376344086021506	No Hit
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCATCACACGA	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AAGGCCCGGCCTCGCCGGGCCGATCCGGAGATCGGAAGAGCACACGTCTG	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCCAGCCCCGCGTCGCACGGATTCGTAGATCGGAAGAGCACACGTCTG	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGC	1	0.5376344086021506	No Hit
AGCAATTAAGGAAACATATGAAAAAATCTCGGAAGAACACAAGTCACAAC	1	0.5376344086021506	No Hit
CGATCTTATACACTCTCTAGCAGCAGATGGGAAGGGCACACGTCTGAACT	1	0.5376344086021506	No Hit
GCGTTTGTAGTCCAACGGTTAGGATAATTGCCTTCCAGATCGGAAGAGCA	1	0.5376344086021506	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCTAGATCGGAAGAGCA	1	0.5376344086021506	No Hit
GTTGCTTCACGACTGGAAGAATGGCCTGATTAGATCGGAAGAGCACACGT	1	0.5376344086021506	No Hit
GGTCCCGAGTTCATTTCTCGGAATGCCCCCCGAGATCGGAAGAGCACACG	1	0.5376344086021506	No Hit
GACACGACTCTCGGCAGCGGATATCTCGGCTCTCGCATCGATGAGATCGG	1	0.5376344086021506	No Hit
AACAAACAGAACTGATAAATTTACACATCGGAAGAGAAAACGTATGAACA	1	0.5376344086021506	No Hit
CCGGATTATGACTGAACGCCTCTAAGATCGGAAGAGCACACGTCTGAACT	1	0.5376344086021506	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	2.150537634408602	0.0	0.0	0.0	0.0
15	2.6881720430107525	0.0	0.0	0.0	0.0
16	3.225806451612903	0.0	0.0	0.0	0.0
17	3.763440860215054	0.0	0.0	0.0	0.0
18	4.301075268817204	0.0	0.0	0.0	0.0
19	6.451612903225806	0.0	0.0	0.0	0.0
20	6.451612903225806	0.0	0.0	0.0	0.0
21	10.21505376344086	0.0	0.0	0.0	0.0
22	12.365591397849462	0.0	0.0	0.0	0.0
23	17.204301075268816	0.0	0.0	0.0	0.0
24	23.655913978494624	0.0	0.0	0.0	0.0
25	38.70967741935484	0.0	0.0	0.0	0.0
26	41.935483870967744	0.0	0.0	0.0	0.0
27	45.16129032258065	0.0	0.0	0.0	0.0
28	57.526881720430104	0.0	0.0	0.0	0.0
29	61.29032258064516	0.0	0.0	0.0	0.0
30	62.903225806451616	0.0	0.0	0.0	0.0
31	64.51612903225806	0.0	0.0	0.0	0.0
32	67.74193548387096	0.0	0.0	0.0	0.0
33	69.35483870967742	0.0	0.0	0.0	0.0
34	69.89247311827957	0.0	0.0	0.0	0.0
35	71.50537634408602	0.0	0.0	0.0	0.0
36	73.65591397849462	0.0	0.0	0.0	0.0
37	76.34408602150538	0.0	0.0	0.0	0.0
38	77.95698924731182	0.0	0.0	0.0	0.0
39	77.95698924731182	0.0	0.0	0.0	0.0
40	79.03225806451613	0.0	0.0	0.0	0.0
41	79.03225806451613	0.0	0.0	0.0	0.0
42	79.56989247311827	0.0	0.0	0.0	0.0
43	79.56989247311827	0.0	0.0	0.0	0.0
44	80.10752688172043	0.0	0.0	0.0	0.0
45	80.10752688172043	0.0	0.0	0.0	0.0
46	80.64516129032258	0.0	0.0	0.0	0.0
47	81.18279569892474	0.0	0.0	0.0	0.0
48	81.18279569892474	0.0	0.0	0.0	0.0
49	81.72043010752688	0.0	0.0	0.0	0.0
50	81.72043010752688	0.0	0.0	0.0	0.0
51	81.72043010752688	0.0	0.0	0.0	0.0
52	81.72043010752688	0.0	0.0	0.0	0.0
53	81.72043010752688	0.0	0.0	0.0	0.0
54	81.72043010752688	0.0	0.0	0.0	0.0
55	81.72043010752688	0.0	0.0	0.0	0.0
56	81.72043010752688	0.0	0.0	0.0	0.0
57	81.72043010752688	0.0	0.0	0.0	0.0
58	81.72043010752688	0.0	0.0	0.0	0.0
59	81.72043010752688	0.0	0.0	0.0	0.0
60	81.72043010752688	0.0	0.0	0.0	0.0
61	81.72043010752688	0.0	0.0	0.0	0.0
62	81.72043010752688	0.0	0.0	0.0	0.0
63	81.72043010752688	0.0	0.0	0.0	0.0
64	81.72043010752688	0.0	0.0	0.0	0.0
65	81.72043010752688	0.0	0.0	0.0	0.0
66	81.72043010752688	0.0	0.0	0.0	0.0
67	81.72043010752688	0.0	0.0	0.0	0.0
68	81.72043010752688	0.0	0.0	0.0	0.0
69	81.72043010752688	0.0	0.0	0.0	0.0
70	81.72043010752688	0.0	0.0	0.0	0.0
71	81.72043010752688	0.0	0.0	0.0	0.0
72	81.72043010752688	0.0	0.0	0.0	0.0
73	81.72043010752688	0.0	0.0	0.0	0.0
74	81.72043010752688	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGCTT	10	0.009481483	79.99999	80
>>END_MODULE
Rejected 9 READS because READLEN < 1
Read 9 spots for SRR11699470.sra
Written 9 spots for SRR11699470.sra
Rejected 9 READS because READLEN < 1
Read 9 spots for SRR11699470.sra
Written 9 spots for SRR11699470.sra
Rejected 9 READS because READLEN < 1
Read 9 spots for SRR11699470.sra
Written 9 spots for SRR11699470.sra
Rejected 9 READS because READLEN < 1
Read 9 spots for SRR11699470.sra
Written 9 spots for SRR11699470.sra
Rejected 9 READS because READLEN < 1
Read 9 spots for SRR11699470.sra
Written 9 spots for SRR11699470.sra
Rejected 9 READS because READLEN < 1
Read 9 spots for SRR11699470.sra
Written 9 spots for SRR11699470.sra
Rejected 9 READS because READLEN < 1
Read 9 spots for SRR11699470.sra
Written 9 spots for SRR11699470.sra
Rejected 9 READS because READLEN < 1
Read 9 spots for SRR11699470.sra
Written 9 spots for SRR11699470.sra
Rejected 9 READS because READLEN < 1
Read 9 spots for SRR11699470.sra
Written 9 spots for SRR11699470.sra
Rejected 9 READS because READLEN < 1
Read 9 spots for SRR11699470.sra
Written 9 spots for SRR11699470.sra
Rejected 9 READS because READLEN < 1
Read 9 spots for SRR11699470.sra
Written 9 spots for SRR11699470.sra
Rejected 9 READS because READLEN < 1
Read 9 spots for SRR11699470.sra
Written 9 spots for SRR11699470.sra
Rejected 9 READS because READLEN < 1
Read 9 spots for SRR11699470.sra
Written 9 spots for SRR11699470.sra
Rejected 9 READS because READLEN < 1
Read 9 spots for SRR11699470.sra
Written 9 spots for SRR11699470.sra
Rejected 9 READS because READLEN < 1
Read 9 spots for SRR11699470.sra
Written 9 spots for SRR11699470.sra
Rejected 9 READS because READLEN < 1
Read 9 spots for SRR11699470.sra
Written 9 spots for SRR11699470.sra
Rejected 9 READS because READLEN < 1
Read 9 spots for SRR11699470.sra
Written 9 spots for SRR11699470.sra
Rejected 9 READS because READLEN < 1
Read 9 spots for SRR11699470.sra
Written 9 spots for SRR11699470.sra
Rejected 9 READS because READLEN < 1
Read 9 spots for SRR11699470.sra
Written 9 spots for SRR11699470.sra
Rejected 15 READS because READLEN < 1
Read 15 spots for SRR11699470.sra
Written 15 spots for SRR11699470.sra
SRR ids: ['SRR11699470.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_gao5ra6z
SRR11699470.sra spots: 186
blocks: [[1, 9], [10, 18], [19, 27], [28, 36], [37, 45], [46, 54], [55, 63], [64, 72], [73, 81], [82, 90], [91, 99], [100, 108], [109, 117], [118, 126], [127, 135], [136, 144], [145, 153], [154, 162], [163, 171], [172, 186]]
SRR11699470 file size 38
SRR11699470 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR11699470 SRR11699470_1.fastq
Input file:	SRR11699470_1.fastq
trimmed:	SRR11699470-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	20
Fri Jun 20 07:03:18 2025 >> started

Fri Jun 20 07:03:18 2025 >> done (0.002s)
186 reads processed; of these:
  9 ( 4.84%) short reads filtered out after trimming by size control
  0 ( 0.00%) empty reads filtered out after trimming by size control
177 (95.16%) reads available; of these:
151 (85.31%) trimmed reads available after processing
 26 (14.69%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  4	  2.26%
 19	  0	  0.00%
 20	  8	  4.52%
 21	  5	  2.82%
 22	 11	  6.21%
 23	 13	  7.34%
 24	 28	 15.82%
 25	  6	  3.39%
 26	  6	  3.39%
 27	 23	 12.99%
 28	  7	  3.95%
 29	  4	  2.26%
 30	  3	  1.69%
 31	  6	  3.39%
 32	  3	  1.69%
 33	  1	  0.56%
 34	  3	  1.69%
 35	  5	  2.82%
 36	  5	  2.82%
 37	  3	  1.69%
 38	  1	  0.56%
 39	  2	  1.13%
 40	  0	  0.00%
 41	  1	  0.56%
 42	  0	  0.00%
 43	  1	  0.56%
 44	  0	  0.00%
 45	  1	  0.56%
 46	  1	  0.56%
 47	  0	  0.00%
 48	  0	  0.00%
 49	  0	  0.00%
 50	  0	  0.00%
 51	  0	  0.00%
 52	  0	  0.00%
 53	  0	  0.00%
 54	  0	  0.00%
 55	  0	  0.00%
 56	  0	  0.00%
 57	  0	  0.00%
 58	  0	  0.00%
 59	  0	  0.00%
 60	  0	  0.00%
 61	  0	  0.00%
 62	  0	  0.00%
 63	  0	  0.00%
 64	  0	  0.00%
 65	  0	  0.00%
 66	  0	  0.00%
 67	  0	  0.00%
 68	  0	  0.00%
 69	  0	  0.00%
 70	  0	  0.00%
 71	  0	  0.00%
 72	  0	  0.00%
 73	  0	  0.00%
 74	  0	  0.00%
 75	  0	  0.00%
 76	  0	  0.00%
 77	  0	  0.00%
 78	  0	  0.00%
 79	  0	  0.00%
 80	  0	  0.00%
 81	  0	  0.00%
 82	  0	  0.00%
 83	  0	  0.00%
 84	  0	  0.00%
 85	  0	  0.00%
 86	 26	 14.69%
177 reads passed initial QC
                                 Started job on |	Jun 20 07:03:48
                             Started mapping on |	Jun 20 07:03:48
                                    Finished on |	Jun 20 07:03:49
       Mapping speed, Million of reads per hour |	0.64

                          Number of input reads |	177
                      Average input read length |	35
                                    UNIQUE READS:
                   Uniquely mapped reads number |	28
                        Uniquely mapped reads % |	15.82%
                          Average mapped length |	24.82
                       Number of splices: Total |	0
            Number of splices: Annotated (sjdb) |	0
                       Number of splices: GT/AG |	0
                       Number of splices: GC/AG |	0
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	0
                      Mismatch rate per base, % |	2.16%
                         Deletion rate per base |	0.00%
                        Deletion average length |	0.00
                        Insertion rate per base |	0.00%
                       Insertion average length |	0.00
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	25
             % of reads mapped to multiple loci |	14.12%
        Number of reads mapped to too many loci |	35
             % of reads mapped to too many loci |	19.77%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	17.51%
                     % of reads unmapped: other |	32.77%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	124	124	124
N_multimapping	25	25	25
N_noFeature	28	28	28
N_ambiguous	0	0	0
UnstrandedReadsAssigned:0 PositiveStrandReadsAssigned:0 NegativeStrandReadsAssigned:0
Dataset is classified positive stranded
MeadianReadLen=27 20thPercentileLength=23 echo kmer=19
SRR11699470 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 72,539
[index] number of k-mers: 56,563,136
[index] number of equivalence classes: 414,827
[quant] running in single-end mode
[quant] will process file 1: SRR11699470-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 177 reads, 20 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 218 rounds

  72540 SRR11699470.ke.tsv
  44303 SRR11699470.se.tsv
 116843 total
==> SRR11699470.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Zm00001eb096110_T001	2067	1968	0	0
Zm00001eb321680_T001	3417	3318	0	0
Zm00001eb323640_T001	3273	3174	0	0
Zm00001eb314140_T001	893	794	0	0
Zm00001eb080260_T001	3529	3430	0	0
Zm00001eb026490_T001	2433	2334	0	0
Zm00001eb026490_T002	2229	2130	0	0
Zm00001eb026490_T003	2220	2121	0	0
Zm00001eb281360_T001	2412	2313	0	0

==> SRR11699470.se.tsv <==
Zm00001eb015280	0
Zm00001eb000610	0
Zm00001eb033210	0
Zm00001eb044610	0
Zm00001eb014360	0
Zm00001eb051320	0
Zm00001eb021860	0
Zm00001eb011690	0
Zm00001eb016840	0
Zm00001eb052060	0
SRR11699470 completed mapping pipeline successfully
