Starting /dee2/code/volunteer_pipeline.sh SRR11699266
    current disk space = 2472243650560
    free memory = 1581307316 
SRR11699266 SRAfilesize
d32763ce06a5ffef0766cdb7af2f85e4  SRR11699266.sra
SRR11699266.sra file validated
SRR11699266 is single end
SRR11699266 is conventional basespace
SRR11699266 read1 length is 76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR11699266_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3955	32.0	32.0	32.0	32.0	32.0
2	31.4315	32.0	32.0	32.0	32.0	32.0
3	31.46725	32.0	32.0	32.0	32.0	32.0
4	31.54925	32.0	32.0	32.0	32.0	32.0
5	31.578	32.0	32.0	32.0	32.0	32.0
6	35.05675	36.0	36.0	36.0	36.0	36.0
7	35.177	36.0	36.0	36.0	36.0	36.0
8	35.1455	36.0	36.0	36.0	36.0	36.0
9	35.02025	36.0	36.0	36.0	36.0	36.0
10-11	35.15025	36.0	36.0	36.0	36.0	36.0
12-13	35.1425	36.0	36.0	36.0	36.0	36.0
14-15	35.033500000000004	36.0	36.0	36.0	36.0	36.0
16-17	35.16	36.0	36.0	36.0	36.0	36.0
18-19	35.14125	36.0	36.0	36.0	36.0	36.0
20-21	35.008250000000004	36.0	36.0	36.0	36.0	36.0
22-23	35.058	36.0	36.0	36.0	36.0	36.0
24-25	35.04025	36.0	36.0	36.0	36.0	36.0
26-27	34.828125	36.0	36.0	36.0	36.0	36.0
28-29	35.002125	36.0	36.0	36.0	36.0	36.0
30-31	34.936125000000004	36.0	36.0	36.0	36.0	36.0
32-33	34.9	36.0	36.0	36.0	36.0	36.0
34-35	34.966499999999996	36.0	36.0	36.0	36.0	36.0
36-37	34.994875	36.0	36.0	36.0	36.0	36.0
38-39	34.88775	36.0	36.0	36.0	36.0	36.0
40-41	34.963499999999996	36.0	36.0	36.0	36.0	36.0
42-43	34.8	36.0	36.0	36.0	34.0	36.0
44-45	34.90325	36.0	36.0	36.0	36.0	36.0
46-47	34.799375	36.0	36.0	36.0	34.0	36.0
48-49	34.716750000000005	36.0	36.0	36.0	34.0	36.0
50-51	34.30925	36.0	36.0	36.0	32.0	36.0
52-53	34.09325	36.0	36.0	36.0	32.0	36.0
54-55	33.72475	36.0	36.0	36.0	29.5	36.0
56-57	32.884	36.0	34.0	36.0	21.0	36.0
58-59	32.196	36.0	32.0	36.0	14.0	36.0
60-61	31.474875	36.0	32.0	36.0	14.0	36.0
62-63	30.81325	36.0	32.0	36.0	14.0	36.0
64-65	29.548125	36.0	20.5	36.0	14.0	36.0
66-67	27.74125	32.0	14.0	36.0	14.0	36.0
68-69	27.235875	32.0	14.0	36.0	14.0	36.0
70-71	26.772624999999998	32.0	14.0	36.0	14.0	36.0
72-73	26.548375	29.5	14.0	36.0	14.0	36.0
74-75	26.546	32.0	14.0	36.0	14.0	36.0
76	24.06225	21.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	2.0
22	4.0
23	9.0
24	10.0
25	22.0
26	44.0
27	66.0
28	132.0
29	171.0
30	252.0
31	351.0
32	470.0
33	692.0
34	1027.0
35	745.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.95	31.8	22.275	24.975
2	22.525000000000002	27.750000000000004	25.8	23.925
3	24.825	34.275	18.125	22.775000000000002
4	26.900000000000002	32.25	19.3	21.55
5	21.95	28.749999999999996	21.175	28.125
6	26.6	25.575	25.575	22.25
7	30.125	32.875	17.7	19.3
8	24.3	28.375	25.674999999999997	21.65
9	24.4	29.625	19.5	26.474999999999998
10-11	30.4625	28.125	22.375	19.037499999999998
12-13	23.8375	24.7875	22.875	28.499999999999996
14-15	29.6875	34.2	19.412499999999998	16.7
16-17	24.575	24.825	30.1875	20.4125
18-19	32.675	25.662499999999998	24.474999999999998	17.1875
20-21	25.525	34.9375	22.112499999999997	17.424999999999997
22-23	30.29507376844211	36.33408352088022	15.641410352588148	17.72943235808952
24-25	28.199999999999996	38.2625	15.6	17.9375
26-27	29.7125	32.9375	16.7875	20.5625
28-29	31.9875	27.775	16.5125	23.724999999999998
30-31	36.4375	34.7125	9.9125	18.9375
32-33	27.925	41.075	11.05	19.950000000000003
34-35	31.924999999999997	32.1125	12.0875	23.875
36-37	26.0	32.65	10.825	30.525000000000002
38-39	21.7375	33.7875	13.2875	31.1875
40-41	23.7375	28.9125	17.2125	30.1375
42-43	26.5125	25.112499999999997	22.375	26.0
44-45	20.1125	31.087500000000002	18.4875	30.312499999999996
46-47	21.575	32.35	14.5875	31.4875
48-49	14.0375	29.4875	16.6	39.875
50-51	15.65	26.450000000000003	19.25	38.65
52-53	16.893835188195574	28.860822808553205	18.794547955483306	35.450794047767914
54-55	17.6375	26.437500000000004	21.525	34.4
56-57	12.562499999999998	28.6875	16.6	42.15
58-59	14.499999999999998	26.787499999999998	17.5375	41.175
60-61	14.787500000000001	28.4375	24.887500000000003	31.887500000000003
62-63	16.5625	27.6125	28.237499999999997	27.5875
64-65	10.925	29.362500000000004	27.625	32.087500000000006
66-67	13.4375	16.650000000000002	33.875	36.0375
68-69	15.587500000000002	17.8625	34.4	32.15
70-71	18.142035508877218	18.86721680420105	35.45886471617904	27.53188297074269
72-73	17.1625	17.424999999999997	33.225	32.1875
74-75	20.474999999999998	12.275	32.3875	34.862500000000004
76	10.575	12.225	41.675000000000004	35.525
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.5
31	0.5
32	0.0
33	0.0
34	0.0
35	0.0
36	0.5
37	1.0
38	1.5
39	5.0
40	9.5
41	20.0
42	29.0
43	48.0
44	97.5
45	189.0
46	250.0
47	403.0
48	670.0
49	668.0
50	552.0
51	529.0
52	447.5
53	334.0
54	279.0
55	219.5
56	147.5
57	104.5
58	74.0
59	53.0
60	22.5
61	11.5
62	10.0
63	6.0
64	2.5
65	1.5
66	0.0
67	0.0
68	0.5
69	1.0
70	1.0
71	1.0
72	0.5
73	0.0
74	0.0
75	0.0
76	0.5
77	0.5
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.025
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0375
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.025
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.27124773960217	62.4
2	4.918625678119349	6.800000000000001
3	1.5551537070524413	3.225
4	0.5786618444846293	1.6
5	0.47016274864376134	1.625
6	0.5424954792043399	2.25
7	0.3978300180831827	1.925
8	0.14466546112115733	0.8
9	0.28933092224231466	1.7999999999999998
>10	0.7956600361663654	12.35
>50	0.0	0.0
>100	0.03616636528028933	5.225
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCC	209	5.225	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAAC	49	1.225	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCA	43	1.075	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACT	38	0.95	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	36	0.8999999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGT	28	0.7000000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTGAGATCGGAAGAGCAC	28	0.7000000000000001	No Hit
AACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAG	25	0.625	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT	23	0.575	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACG	22	0.5499999999999999	TruSeq Adapter, Index 7 (97% over 35bp)
CTAACGAACGAACGATTTGAAGATCGGAAGAGCACACGTCTGAACTCCAG	22	0.5499999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGCTATAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAC	22	0.5499999999999999	TruSeq Adapter, Index 7 (97% over 35bp)
TAATTCATGATCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	22	0.5499999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCA	21	0.525	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	19	0.475	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCCTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACT	17	0.42500000000000004	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAC	14	0.35000000000000003	TruSeq Adapter, Index 7 (97% over 35bp)
GCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACAC	12	0.3	No Hit
TCCTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTC	12	0.3	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGTAA	10	0.25	TruSeq Adapter, Index 7 (97% over 35bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCTAGATCGGAAGAGCA	10	0.25	No Hit
TCCTGCGGCAAAATAGCTCGATGCCAGAATAGATCGGAAGAGCACACGTC	10	0.25	No Hit
AGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGT	9	0.22499999999999998	TruSeq Adapter, Index 7 (97% over 35bp)
CGACACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACT	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCAGATCGGAAGAGCACACG	9	0.22499999999999998	No Hit
CGACACGACTCTCGGCAACGAGATCGGAAGAGCACACGTCTGAACTCCAG	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGTAAT	9	0.22499999999999998	TruSeq Adapter, Index 7 (97% over 35bp)
ACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATTCTGGTGTCCTAGGCGTAGAGGAACCACAAGATCGGAAGAGCACACG	9	0.22499999999999998	No Hit
ACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTGAGATCGGAAGAGCAC	8	0.2	No Hit
CGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGTA	8	0.2	TruSeq Adapter, Index 7 (97% over 35bp)
TATTCTGGTGTCCTAGGCGTAGAGGAACCACACCAGATCGGAAGAGCACA	7	0.17500000000000002	No Hit
TGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGT	7	0.17500000000000002	No Hit
TCAAAAGAGGAAAGGCTTGCGGTGGATACCTAGGCACCCAGAGATCGGAA	7	0.17500000000000002	No Hit
GCGGCAAAATAGCTCGATGCCAGAATAGATCGGAAGAGCACACGTCTGAA	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACTC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATGCAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGA	7	0.17500000000000002	No Hit
AGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACA	7	0.17500000000000002	No Hit
GGGGATGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA	7	0.17500000000000002	TruSeq Adapter, Index 7 (100% over 35bp)
ACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGT	7	0.17500000000000002	TruSeq Adapter, Index 7 (97% over 35bp)
GAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAC	7	0.17500000000000002	TruSeq Adapter, Index 7 (97% over 35bp)
CCTGCGGCAAAATAGCTCGATGCCAGAATAGATCGGAAGAGCACACGTCT	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACACGACTCTCGGCAACGGAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGCACGGATTCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA	6	0.15	TruSeq Adapter, Index 7 (100% over 35bp)
CGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA	6	0.15	TruSeq Adapter, Index 7 (100% over 35bp)
TCCAGCCCCGCGTCGCACGGATTCGTAGATCGGAAGAGCACACGTCTGAA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGCGGCAAAATAGCTCGATGCCAGAATAGATCGGAAGAGCACACGTCTG	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGT	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CACCCCAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAGGTCCTGCGGCAAAATAGCTCGATGCCAGAATAGATCGGAAGAGCAC	6	0.15	No Hit
CCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAATTCATGATCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGACCCCAGGTCAGTCGGGACTAGATCGGAAGAGCACACGTCTGAACTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCAC	6	0.15	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTCAGATCGGAAGAGCACACGTC	6	0.15	No Hit
CACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCAGATCGGAAGAGC	6	0.15	No Hit
CACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGCCCCGCGTCGCACGGATTCGTAGATCGGAAGAGCACACGTCTGAACT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCAGTGATGTATGCAGCTGAGGCATCCTAACGAACGAACGATTTGAACAG	5	0.125	No Hit
TGCAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGC	5	0.125	No Hit
GCGAGCGTAGTTCAGTGGTAAAACATCTCCTTGCCAAGGAGAGATCGGAA	5	0.125	No Hit
ACACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCAGATCGGAAGAG	5	0.125	No Hit
AAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTAACGAACGAACGATTTGAATAGATCGGAAGAGCACACGTCTGAACTCC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACACGACTCTCGGCAACGGATATCTCGGCAGATCGGAAGAGCACACGT	5	0.125	No Hit
CGACACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCAGATCGGAAG	5	0.125	No Hit
GACACGACTCTCGGCAACGGATATCTCGAGATCGGAAGAGCACACGTCTG	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACACGACTCTCGGCAACAGATCGGAAGAGCACACGTCTGAACTCCAGT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAC	5	0.125	TruSeq Adapter, Index 7 (97% over 35bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.025	0.0	0.0	0.0	0.0
2	0.1	0.0	0.0	0.0	0.0
3	0.15	0.0	0.0	0.0	0.0
4	0.2	0.0	0.0	0.0	0.0
5	0.3	0.0	0.0	0.0	0.0
6	0.425	0.0	0.0	0.0	0.0
7	0.475	0.0	0.0	0.0	0.0
8	0.675	0.0	0.0	0.0	0.0
9	1.05	0.0	0.0	0.0	0.0
10	1.475	0.0	0.0	0.0	0.0
11	2.125	0.0	0.0	0.0	0.0
12	3.1	0.0	0.0	0.0	0.0
13	4.375	0.0	0.0	0.0	0.0
14	6.85	0.0	0.0	0.0	0.0
15	8.225	0.0	0.0	0.0	0.0
16	10.05	0.0	0.0	0.0	0.0
17	12.85	0.0	0.0	0.0	0.0
18	15.35	0.0	0.0	0.0	0.0
19	17.875	0.0	0.0	0.0	0.0
20	21.475	0.0	0.0	0.0	0.0
21	25.525	0.0	0.0	0.0	0.0
22	30.975	0.0	0.0	0.0	0.0
23	40.675	0.0	0.0	0.0	0.0
24	44.675	0.0	0.0	0.0	0.0
25	56.65	0.0	0.0	0.0	0.0
26	60.25	0.0	0.0	0.0	0.0
27	62.8	0.0	0.0	0.0	0.0
28	65.3	0.0	0.0	0.0	0.0
29	67.9	0.0	0.0	0.0	0.0
30	70.275	0.0	0.0	0.0	0.0
31	72.825	0.0	0.0	0.0	0.0
32	75.05	0.0	0.0	0.0	0.0
33	77.25	0.0	0.0	0.0	0.0
34	79.075	0.0	0.0	0.0	0.0
35	81.25	0.0	0.0	0.0	0.0
36	84.35	0.0	0.0	0.0	0.0
37	86.3	0.0	0.0	0.0	0.0
38	87.775	0.0	0.0	0.0	0.0
39	88.65	0.0	0.0	0.0	0.0
40	89.775	0.0	0.0	0.0	0.0
41	90.55	0.0	0.0	0.0	0.0
42	91.525	0.0	0.0	0.0	0.0
43	91.9	0.0	0.0	0.0	0.0
44	92.325	0.0	0.0	0.0	0.0
45	92.8	0.0	0.0	0.0	0.0
46	93.2	0.0	0.0	0.0	0.0
47	93.525	0.0	0.0	0.0	0.0
48	93.75	0.0	0.0	0.0	0.0
49	94.075	0.0	0.0	0.0	0.0
50	94.15	0.0	0.0	0.0	0.0
51	94.2	0.0	0.0	0.0	0.0
52	94.275	0.0	0.0	0.0	0.0
53	94.325	0.0	0.0	0.0	0.0
54	94.375	0.0	0.0	0.0	0.0
55	94.425	0.0	0.0	0.0	0.0
56	94.425	0.0	0.0	0.0	0.0
57	94.425	0.0	0.0	0.0	0.0
58	94.475	0.0	0.0	0.0	0.0
59	94.475	0.0	0.0	0.0	0.0
60	94.475	0.0	0.0	0.0	0.0
61	94.475	0.0	0.0	0.0	0.0
62	94.475	0.0	0.0	0.0	0.0
63	94.475	0.0	0.0	0.0	0.0
64	94.475	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAGGAT	15	0.0020658788	70.00001	20
CCGTTGT	15	0.0020658788	70.00001	2
TAGTCTA	15	0.0020658788	70.00001	8
GGTCAGG	15	0.0020658788	70.00001	18
TGTAGTC	15	0.0020658788	70.00001	6
TCCGTTG	15	0.0020658788	70.00001	1
TTGGTCA	15	0.0020658788	70.00001	16
CGTTGTA	15	0.0020658788	70.00001	3
AGTCTAG	15	0.0020658788	70.00001	9
GTCAGGA	15	0.0020658788	70.00001	19
GTAGTCT	15	0.0020658788	70.00001	7
GTTGTAG	15	0.0020658788	70.00001	4
TGGTCAG	25	2.3570374E-6	70.0	17
TTGTAGT	25	2.3570374E-6	70.0	5
TTCTGCT	20	0.0064587677	52.5	69
TCTGCTT	20	0.0064587677	52.5	70
TGTAGTT	20	0.0064587677	52.5	6
CAGGATA	20	0.0064587677	52.5	21
TTTGAAC	50	1.4254966E-4	35.0	16
TTGAACA	50	1.4254966E-4	35.0	17
>>END_MODULE
Rejected 232066 READS because READLEN < 1
Read 232066 spots for SRR11699266.sra
Written 232066 spots for SRR11699266.sra
Rejected 232066 READS because READLEN < 1
Read 232066 spots for SRR11699266.sra
Written 232066 spots for SRR11699266.sra
Rejected 232066 READS because READLEN < 1
Read 232066 spots for SRR11699266.sra
Written 232066 spots for SRR11699266.sra
Rejected 232066 READS because READLEN < 1
Read 232066 spots for SRR11699266.sra
Written 232066 spots for SRR11699266.sra
Rejected 232066 READS because READLEN < 1
Read 232066 spots for SRR11699266.sra
Written 232066 spots for SRR11699266.sra
Rejected 232066 READS because READLEN < 1
Read 232066 spots for SRR11699266.sra
Written 232066 spots for SRR11699266.sra
Rejected 232066 READS because READLEN < 1
Read 232066 spots for SRR11699266.sra
Written 232066 spots for SRR11699266.sra
Rejected 232066 READS because READLEN < 1
Read 232066 spots for SRR11699266.sra
Written 232066 spots for SRR11699266.sra
Rejected 232066 READS because READLEN < 1
Read 232066 spots for SRR11699266.sra
Written 232066 spots for SRR11699266.sra
Rejected 232066 READS because READLEN < 1
Read 232066 spots for SRR11699266.sra
Written 232066 spots for SRR11699266.sra
Rejected 232066 READS because READLEN < 1
Read 232066 spots for SRR11699266.sra
Written 232066 spots for SRR11699266.sra
Rejected 232066 READS because READLEN < 1
Read 232066 spots for SRR11699266.sra
Written 232066 spots for SRR11699266.sra
Rejected 232066 READS because READLEN < 1
Read 232066 spots for SRR11699266.sra
Written 232066 spots for SRR11699266.sra
Rejected 232066 READS because READLEN < 1
Read 232066 spots for SRR11699266.sra
Written 232066 spots for SRR11699266.sra
Rejected 232072 READS because READLEN < 1
Read 232072 spots for SRR11699266.sra
Written 232072 spots for SRR11699266.sra
Rejected 232066 READS because READLEN < 1
Read 232066 spots for SRR11699266.sra
Written 232066 spots for SRR11699266.sra
Rejected 232066 READS because READLEN < 1
Read 232066 spots for SRR11699266.sra
Written 232066 spots for SRR11699266.sra
Rejected 232066 READS because READLEN < 1
Read 232066 spots for SRR11699266.sra
Written 232066 spots for SRR11699266.sra
Rejected 232066 READS because READLEN < 1
Read 232066 spots for SRR11699266.sra
Written 232066 spots for SRR11699266.sra
Rejected 232066 READS because READLEN < 1
Read 232066 spots for SRR11699266.sra
Written 232066 spots for SRR11699266.sra
SRR ids: ['SRR11699266.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_xxcld3pf
SRR11699266.sra spots: 4641326
blocks: [[1, 232066], [232067, 464132], [464133, 696198], [696199, 928264], [928265, 1160330], [1160331, 1392396], [1392397, 1624462], [1624463, 1856528], [1856529, 2088594], [2088595, 2320660], [2320661, 2552726], [2552727, 2784792], [2784793, 3016858], [3016859, 3248924], [3248925, 3480990], [3480991, 3713056], [3713057, 3945122], [3945123, 4177188], [4177189, 4409254], [4409255, 4641326]]
SRR11699266 file size 881677
SRR11699266 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR11699266 SRR11699266_1.fastq
Input file:	SRR11699266_1.fastq
trimmed:	SRR11699266-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	20
Wed Jun 18 06:12:39 2025 >> started

Wed Jun 18 06:12:41 2025 >> done (1.959s)
4641326 reads processed; of these:
 823968 (17.75%) short reads filtered out after trimming by size control
   2135 ( 0.05%) empty reads filtered out after trimming by size control
3815223 (82.20%) reads available; of these:
3731816 (97.81%) trimmed reads available after processing
  83407 ( 2.19%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	 116076	  3.04%
 19	 175680	  4.60%
 20	 193547	  5.07%
 21	 280541	  7.35%
 22	 452325	 11.86%
 23	 187861	  4.92%
 24	 540568	 14.17%
 25	 195726	  5.13%
 26	 126532	  3.32%
 27	 131992	  3.46%
 28	 110725	  2.90%
 29	 112625	  2.95%
 30	 147240	  3.86%
 31	 118507	  3.11%
 32	 104719	  2.74%
 33	 100138	  2.62%
 34	 100805	  2.64%
 35	 141641	  3.71%
 36	  93484	  2.45%
 37	  66782	  1.75%
 38	  48564	  1.27%
 39	  45858	  1.20%
 40	  36041	  0.94%
 41	  40118	  1.05%
 42	  26466	  0.69%
 43	  21778	  0.57%
 44	  15473	  0.41%
 45	      0	  0.00%
 46	      0	  0.00%
 47	      0	  0.00%
 48	      0	  0.00%
 49	      0	  0.00%
 50	      0	  0.00%
 51	      0	  0.00%
 52	      0	  0.00%
 53	      0	  0.00%
 54	      0	  0.00%
 55	      0	  0.00%
 56	      0	  0.00%
 57	      0	  0.00%
 58	      0	  0.00%
 59	      0	  0.00%
 60	      0	  0.00%
 61	      0	  0.00%
 62	      0	  0.00%
 63	      0	  0.00%
 64	      0	  0.00%
 65	      0	  0.00%
 66	      0	  0.00%
 67	      0	  0.00%
 68	      0	  0.00%
 69	      0	  0.00%
 70	      1	  0.00%
 71	      0	  0.00%
 72	      0	  0.00%
 73	      0	  0.00%
 74	      0	  0.00%
 75	      3	  0.00%
 76	  83407	  2.19%
3815223 reads passed initial QC


criterion=sequence-density
sequence-density=2.06
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=14
prefix-density=0.01
prefix-fanout=1.0
sequence=TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCTCACCCGAAAGACCCGGGTT


criterion=fanout-score
sequence-density=0.09
sequence-density-rank=18
fanout-score=19.25
fanout-score-rank=1
prefix-density=1.27
prefix-fanout=1.3
sequence=GTCAGGATAGCTCAGTTGGTAGAGCAGAGGACTG
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	Jun 18 06:12:52
                             Started mapping on |	Jun 18 06:12:52
                                    Finished on |	Jun 18 06:13:04
       Mapping speed, Million of reads per hour |	1144.57

                          Number of input reads |	3815223
                      Average input read length |	27
                                    UNIQUE READS:
                   Uniquely mapped reads number |	455220
                        Uniquely mapped reads % |	11.93%
                          Average mapped length |	24.08
                       Number of splices: Total |	9949
            Number of splices: Annotated (sjdb) |	4244
                       Number of splices: GT/AG |	9257
                       Number of splices: GC/AG |	494
                       Number of splices: AT/AC |	7
               Number of splices: Non-canonical |	191
                      Mismatch rate per base, % |	1.16%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.43
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.21
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	456737
             % of reads mapped to multiple loci |	11.97%
        Number of reads mapped to too many loci |	722204
             % of reads mapped to too many loci |	18.93%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.42%
                     % of reads unmapped: other |	50.74%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	2903266	2903266	2903266
N_multimapping	456737	456737	456737
N_noFeature	373653	387141	439008
N_ambiguous	2801	42	46
UnstrandedReadsAssigned:78766 PositiveStrandReadsAssigned:68037 NegativeStrandReadsAssigned:16166
Dataset is classified unstranded
MeadianReadLen=24 20thPercentileLength=21 echo kmer=19
SRR11699266 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 72,539
[index] number of k-mers: 56,563,136
[index] number of equivalence classes: 414,827
[quant] running in single-end mode
[quant] will process file 1: SRR11699266-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,815,223 reads, 740,435 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,261 rounds

  72540 SRR11699266.ke.tsv
  44303 SRR11699266.se.tsv
 116843 total
==> SRR11699266.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Zm00001eb096110_T001	2067	1968	1	0.732744
Zm00001eb321680_T001	3417	3318	3	1.30383
Zm00001eb323640_T001	3273	3174	0	0
Zm00001eb314140_T001	893	794	0	0
Zm00001eb080260_T001	3529	3430	0	0
Zm00001eb026490_T001	2433	2334	0	0
Zm00001eb026490_T002	2229	2130	0	0
Zm00001eb026490_T003	2220	2121	0	0
Zm00001eb281360_T001	2412	2313	2	1.2469

==> SRR11699266.se.tsv <==
Zm00001eb015280	0
Zm00001eb000610	0
Zm00001eb033210	0
Zm00001eb044610	0
Zm00001eb014360	0
Zm00001eb051320	2
Zm00001eb021860	0
Zm00001eb011690	0
Zm00001eb016840	0
Zm00001eb052060	0
SRR11699266 completed mapping pipeline successfully
