Starting /dee2/code/volunteer_pipeline.sh SRR11699360
    current disk space = 2462525202432
    free memory = 973413904 
SRR11699360 SRAfilesize
914372b611ea5b27d598af1a703e1da0  SRR11699360.sra
SRR11699360.sra file validated
SRR11699360 is single end
SRR11699360 is conventional basespace
SRR11699360 read1 length is 86 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR11699360_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	348
Sequences flagged as poor quality	0
Sequence length	86
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.729885057471265	32.0	32.0	32.0	32.0	32.0
2	31.00574712643678	32.0	32.0	32.0	32.0	32.0
3	31.387931034482758	32.0	32.0	32.0	32.0	32.0
4	31.086206896551722	32.0	32.0	32.0	32.0	32.0
5	31.436781609195403	32.0	32.0	32.0	32.0	32.0
6	34.20977011494253	36.0	36.0	36.0	32.0	36.0
7	34.39080459770115	36.0	36.0	36.0	32.0	36.0
8	34.71264367816092	36.0	36.0	36.0	32.0	36.0
9	34.74425287356322	36.0	36.0	36.0	32.0	36.0
10-11	34.85057471264368	36.0	36.0	36.0	36.0	36.0
12-13	34.64798850574713	36.0	36.0	36.0	32.0	36.0
14-15	34.4051724137931	36.0	36.0	36.0	32.0	36.0
16-17	34.47557471264368	36.0	36.0	36.0	32.0	36.0
18-19	34.74425287356322	36.0	36.0	36.0	34.0	36.0
20-21	34.360632183908045	36.0	36.0	36.0	29.5	36.0
22-23	34.185344827586206	36.0	36.0	36.0	28.5	36.0
24-25	34.80603448275862	36.0	36.0	36.0	34.0	36.0
26-27	34.00574712643678	36.0	36.0	36.0	29.5	36.0
28-29	33.518678160919535	36.0	36.0	36.0	21.0	36.0
30-31	33.54597701149426	36.0	36.0	36.0	21.0	36.0
32-33	33.60201149425288	36.0	36.0	36.0	21.0	36.0
34-35	33.22844827586207	36.0	36.0	36.0	17.5	36.0
36-37	33.326149425287355	36.0	36.0	36.0	17.5	36.0
38-39	33.298850574712645	36.0	36.0	36.0	17.5	36.0
40-41	32.98419540229885	36.0	36.0	36.0	14.0	36.0
42-43	33.23850574712644	36.0	36.0	36.0	17.5	36.0
44-45	33.26149425287356	36.0	36.0	36.0	17.5	36.0
46-47	32.991379310344826	36.0	36.0	36.0	14.0	36.0
48-49	32.86494252873563	36.0	36.0	36.0	14.0	36.0
50-51	32.88649425287356	36.0	34.0	36.0	14.0	36.0
52-53	32.69827586206897	36.0	36.0	36.0	14.0	36.0
54-55	32.62931034482759	36.0	34.0	36.0	14.0	36.0
56-57	32.145114942528735	36.0	32.0	36.0	14.0	36.0
58-59	31.73419540229885	36.0	32.0	36.0	14.0	36.0
60-61	31.583333333333332	36.0	32.0	36.0	14.0	36.0
62-63	30.722701149425287	36.0	27.0	36.0	14.0	36.0
64-65	30.057471264367816	36.0	24.0	36.0	14.0	36.0
66-67	29.88074712643678	36.0	24.0	36.0	14.0	36.0
68-69	30.495689655172413	36.0	29.5	36.0	14.0	36.0
70-71	30.633620689655174	36.0	27.0	36.0	14.0	36.0
72-73	31.761494252873565	36.0	32.0	36.0	14.0	36.0
74-75	31.64655172413793	36.0	32.0	36.0	14.0	36.0
76-77	31.283045977011497	36.0	32.0	36.0	14.0	36.0
78-79	31.40948275862069	36.0	32.0	36.0	14.0	36.0
80-81	31.448275862068964	36.0	32.0	36.0	14.0	36.0
82-83	31.530172413793103	36.0	32.0	36.0	14.0	36.0
84-85	31.6867816091954	36.0	34.0	36.0	14.0	36.0
86	30.560344827586206	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	0.0
21	1.0
22	6.0
23	10.0
24	11.0
25	8.0
26	9.0
27	6.0
28	9.0
29	13.0
30	13.0
31	14.0
32	18.0
33	21.0
34	71.0
35	137.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.862068965517242	24.42528735632184	30.45977011494253	19.25287356321839
2	27.011494252873565	18.39080459770115	16.091954022988507	38.50574712643678
3	27.298850574712645	24.71264367816092	14.080459770114942	33.90804597701149
4	43.103448275862064	18.39080459770115	15.229885057471265	23.275862068965516
5	22.413793103448278	24.137931034482758	31.321839080459768	22.126436781609197
6	19.25287356321839	20.977011494252874	38.50574712643678	21.26436781609195
7	49.42528735632184	11.494252873563218	19.82758620689655	19.25287356321839
8	19.54022988505747	19.25287356321839	41.37931034482759	19.82758620689655
9	21.26436781609195	41.09195402298851	18.67816091954023	18.96551724137931
10-11	32.90229885057471	17.67241379310345	31.752873563218394	17.67241379310345
12-13	21.26436781609195	15.517241379310345	29.454022988505745	33.764367816091955
14-15	33.33333333333333	33.47701149425287	17.385057471264368	15.804597701149426
16-17	23.419540229885058	24.856321839080458	26.00574712643678	25.718390804597703
18-19	40.94827586206897	25.57471264367816	16.23563218390805	17.24137931034483
20-21	26.580459770114945	25.718390804597703	27.011494252873565	20.689655172413794
22-23	33.47701149425287	36.7816091954023	15.229885057471265	14.511494252873563
24-25	28.879310344827587	43.96551724137931	14.798850574712644	12.35632183908046
26-27	24.568965517241377	38.50574712643678	20.833333333333336	16.091954022988507
28-29	29.022988505747126	25.0	21.551724137931032	24.42528735632184
30-31	39.6551724137931	30.17241379310345	11.925287356321839	18.24712643678161
32-33	27.298850574712645	40.37356321839081	10.775862068965516	21.551724137931032
34-35	35.63218390804598	34.770114942528735	10.919540229885058	18.67816091954023
36-37	32.90229885057471	31.60919540229885	9.051724137931034	26.436781609195403
38-39	25.287356321839084	39.94252873563218	9.913793103448276	24.856321839080458
40-41	18.82183908045977	36.06321839080459	11.206896551724139	33.90804597701149
42-43	27.155172413793103	26.14942528735632	20.545977011494255	26.14942528735632
44-45	21.26436781609195	25.43103448275862	18.53448275862069	34.770114942528735
46-47	23.419540229885058	34.339080459770116	14.942528735632186	27.298850574712645
48-49	17.67241379310345	26.867816091954023	20.833333333333336	34.62643678160919
50-51	11.063218390804598	34.48275862068966	18.53448275862069	35.91954022988506
52-53	15.373563218390803	29.454022988505745	18.53448275862069	36.637931034482754
54-55	15.373563218390803	22.701149425287355	22.270114942528735	39.6551724137931
56-57	15.086206896551724	35.48850574712644	15.517241379310345	33.90804597701149
58-59	14.080459770114942	32.4712643678161	17.52873563218391	35.91954022988506
60-61	15.948275862068966	31.46551724137931	16.091954022988507	36.49425287356322
62-63	12.931034482758621	34.339080459770116	17.67241379310345	35.05747126436782
64-65	13.505747126436782	36.637931034482754	21.982758620689655	27.873563218390807
66-67	11.637931034482758	23.850574712643677	27.155172413793103	37.35632183908046
68-69	15.804597701149426	21.839080459770116	28.879310344827587	33.47701149425287
70-71	15.373563218390803	20.545977011494255	34.05172413793103	30.028735632183906
72-73	23.132183908045977	18.103448275862068	27.586206896551722	31.178160919540232
74-75	18.24712643678161	18.39080459770115	30.17241379310345	33.189655172413794
76-77	23.563218390804597	13.218390804597702	34.05172413793103	29.166666666666668
78-79	12.931034482758621	10.919540229885058	37.64367816091954	38.50574712643678
80-81	18.96551724137931	13.07471264367816	42.672413793103445	25.287356321839084
82-83	16.23563218390805	18.103448275862068	38.793103448275865	26.867816091954023
84-85	16.379310344827587	22.126436781609197	36.35057471264368	25.143678160919542
86	14.942528735632186	22.413793103448278	43.39080459770115	19.25287356321839
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	1.0
17	1.0
18	0.5
19	0.5
20	0.5
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.5
32	0.5
33	0.5
34	1.0
35	1.0
36	1.0
37	1.0
38	1.0
39	0.5
40	0.0
41	2.0
42	5.5
43	7.0
44	8.5
45	14.0
46	18.0
47	24.0
48	38.0
49	45.5
50	45.0
51	44.5
52	37.0
53	26.0
54	21.5
55	15.0
56	10.0
57	11.0
58	10.0
59	6.5
60	3.0
61	2.0
62	1.5
63	2.5
64	4.0
65	2.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	348.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.65517241379311
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.11538461538461	87.06896551724138
2	1.6025641025641024	2.8735632183908044
3	0.641025641025641	1.7241379310344827
4	0.3205128205128205	1.1494252873563218
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.3205128205128205	7.183908045977011
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	25	7.183908045977011	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGTTTGTAGTCCAACGGTTAGGATAATTGCCTTCCAGATCGGAAGAGCA	4	1.1494252873563218	No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	3	0.8620689655172413	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACACGACTCTCGGCAACGGATATCTCGAGATCGGAAGAGCACACGTCT	3	0.8620689655172413	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACGACTCTCGGCAACGGATATCAGATCGGAAGAGCACACGTCTGAACT	2	0.5747126436781609	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTCGGGGGTCCCGGCCCCGAACCCGTCGGCAGATCGGAAGAGCACACGT	2	0.5747126436781609	No Hit
GCGACCCCAGGTCAGTCGGGACTACCCGCTGAGTAGATCGGAAGAGCACA	2	0.5747126436781609	No Hit
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCG	2	0.5747126436781609	TruSeq Adapter, Index 10 (97% over 36bp)
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCTTCAGATCGGAAGAGCAC	2	0.5747126436781609	No Hit
GCGCCCGAAGCCTTCTGGCGGAGGGCACGTCTGCCTGGGCGTCACGCCAG	1	0.28735632183908044	No Hit
GTGCTCTGAATGTCAAAGTGAAGAAATTAGATCGGAAGAGCGCACGTCTG	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGACACGACTCTCGGCAACGGATATCTCGGCTAGATCGGAAGAGCACACG	1	0.28735632183908044	No Hit
AGTTAACTAGTTATCTGAACCGTTAAGATCGGAAGAGCACACGTCTGAAC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATAAGACTAAGAATAAAACAATCCAGATCGGAAGAGCAACGTCTGAACTC	1	0.28735632183908044	No Hit
GCGGTCTCTTCCACTCAGATCGGAAGAGCACACGTCTGAACTCCATCACA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AAGGCTAGTTAGCATTGGCACGTGAGATCGGAAGGGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TCCGTTGTAGTCTAGGTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTACCGCGAGGGAAAGATGAAAAGGACTTTGAAAAGAGAGATCGGAAGA	1	0.28735632183908044	No Hit
TCCGTTGTAGTCTAGCTGGTGAGGATATTCTCGTATCAGATCACTAGAGC	1	0.28735632183908044	No Hit
AGAAGGATGGAGGATGCAGACATTAGGTCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GGGGATGTAGCTCAGATCGGAAGGGCACACGTCTGAACTCCAGTCACTCG	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CACGTCTGCCTGGGCGTCACGCAGATCGGAAGAGCACACGTCTGAATCCA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GGCCGGGTCGTGAGATCAGAAGAGCACACGTCTGAACTCCAGTCACACGA	1	0.28735632183908044	TruSeq Adapter, Index 5 (97% over 35bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAGATCGGAAGAGCACACGTCTGAA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCTGATCTGGTCTTAAGAAGCCAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGAACCAAGCCAGAATTCTGATCCTCAGATCGGAAGAGCAACGTCAGA	1	0.28735632183908044	No Hit
GCGATGTAGAGGCGTAGGGGGCCGAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTCGCGATTCCCGGTTACTCACCAGATCGGAGGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GCGGCCGTCGGTGCAGATCTTGGTAGATCGGGAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CAACTGAATCCATAGACCAAGAAGATAGGAAGACCACACTTCACAACTCA	1	0.28735632183908044	No Hit
AGGACTACCTAGCTCGACGCCAAGATCGGAAGGGCACACGTCTGAACTCC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TTGTGCTCAACTATTATGGATGAGATCGGAAGAGCACGCGTCTGAACTCC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GCGACCCCAGGTCAGTCGGGACTACCCGCTAGATCGGAAGAGCACACGTC	1	0.28735632183908044	No Hit
AGCGAACAAGTACCGCGAGATCGGAAGAGCACACGTCTGAACTCCGTCAC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TAATCAGGCGATTGTGGGTCCGTGTCCCACCGGGCGTGCCAAGATCGGAA	1	0.28735632183908044	No Hit
GGGATGGCCTACAGGTTGTCTGACAGGCAGATCGGAAGAGCACACATCTG	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TTCCGAGCGAGTCTCTGGGATCTCAGATCGGAAGAGCACACGTCTGAAGT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TCGATCGGCATACTTCATATGTGCAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGGACCGTTGTCGCCCGCGCCGGCGAGATCGGAAGAGCACTCGTCTGA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CTCTCGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTTCACACGACA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
CTCGATGATCCGCTCGATGACGACAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TACCGTCCTAGTCTCAACCATAAACGAAGATCGGAAGAGCACACGTCTGA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGCGTGGACCGTTGTCGCCCGCGCCGGCGGCAGATCGGAAGAGCACA	1	0.28735632183908044	No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGAGATCGGAAGAGCACACG	1	0.28735632183908044	No Hit
GACACGACTCTCGGCAACGGATATCTCGAGATCGGAAAGAGCACACGTCT	1	0.28735632183908044	No Hit
CCATCAGAACTCCGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTGA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TCCCTGTCCACTCTACAAATAGTAGATCGGAAGAGCACACGTCTGAACTC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGCCGGATCGCAAGCGGAGCC	1	0.28735632183908044	No Hit
GCCCCTCCTTTTGGCTCCAAGGCCCGGCCAGATCGGAAGAGCACACGTCT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACCAATCTAGGAAAACGATATCTCGTCACACCCATCGATAACAACCGAA	1	0.28735632183908044	No Hit
GGTGGCTGTAGTTTAGTGGTTAGAATTCTAGATCGGAAGAGCACACGTCT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAATCTCGGAAGAGATCGCTTCAGC	1	0.28735632183908044	No Hit
TAGTACGAGAGGAACCAGATCGGAAGAGCACACGTCTGAACTCCAATCAC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CGTAGACGATGGCTTACAAGAGAAAGATCGGAAGGGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AGACTCCAGCTAAGGGTTAGGCCTGAGCCTAGATCGGAAGAGCACAAGTC	1	0.28735632183908044	No Hit
CGACACGACTCTCGGCAACGGATATCAGATCTGAAGAGAACACGTCTGAA	1	0.28735632183908044	No Hit
GACACGACTCTCGGCAACGGATATCTCGGCCCTAGATCGGAAGAGCACAC	1	0.28735632183908044	No Hit
ACAGATTTAATCTACATGATCAGAAATAACAACTCAACATATTAAAATCA	1	0.28735632183908044	No Hit
TCCGTTGTAGTCTAGATGGTCAGGAGGTACGAAGAGAACACTTCAGCACT	1	0.28735632183908044	No Hit
GGACTAGGGAAGGACTTGGCGAGTAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGATGTTCTGGGCCGCACGCGCGCTACAGATCGGAAGAGCACACGTCTG	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGCATCGATGAAGGACGTAGCAGATCGGAAGAGCAGACGTCTGAACTC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AATCATGTGGGCTTTAGATCGGAAGAGCACACGTCTGAACTCCATCACAC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GCGCTTGTAGCTCAGTGGAGATCGGAGGAGCACACGTCTGAACTCCAGTC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGGGCACACGTCTGA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TGAAGGTCGCTAGGTTGTACACGCAGATCGGAAGAGCACACGCTGAACTC	1	0.28735632183908044	No Hit
TCCGTTGTAGTCTAGCTGGTAAGGATATTAGCGCAGGAGCGCACTACTCA	1	0.28735632183908044	No Hit
ATTACGGTCTATAGTGCGAAACATAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATAGACTGTGAGAAGACACCGAGCAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATGTGCTCTGAATTTGCAGTAGATCGGAAGAGCACACGTCTTAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TCCGTTGTAGTCTAGCTGGTGAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGTCTAA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GACACGACTCTCGGCAACGGATATCTAGGCTCTCAGATCGGAAGAGAACA	1	0.28735632183908044	No Hit
GCCACGATCCACTGAGATCCAGCCCCGCGTCGCACGGATTCGTAGATCGG	1	0.28735632183908044	No Hit
AAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGAGATGGGAAGAG	1	0.28735632183908044	No Hit
AACTTTAAATAGGTAGGACGGCAGATCGGAAGAGCACACGTCTGAACTCC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATGGGAATCTCTCTGATGCTTAGATCGGAAGAGCACACGTCTGAACTCC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTTATTTTTTGTGGGGGAGGACTCAAGATCGGAAGAGCACACGTCTGAA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCTTTTCGTCCGTTAGATCTAGAGATCGGGAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CGGCCCGCCAACCCTGGAATCAGATCGGAAGAGCAACGTCTGAACTCCAG	1	0.28735632183908044	No Hit
TGAAAGACACCTAATCCATTCATAATTCAAGACTGAGAACAAGATCGGAA	1	0.28735632183908044	No Hit
TCGCACGGATTCGTAGATCGGAAGAGCAACGTCTGAACTCCAGTCACACG	1	0.28735632183908044	TruSeq Adapter, Index 15 (95% over 22bp)
ACGTCTGCCTGGGCGTTACGCAGATCGGAGGAGCACACGTCTGAACTCCA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GTAAGATTTGATTTGATTGGAATTAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTTAGAGGGACTATGGCCGTTTAGGCCACGGAAGTTTGAGGCAGATCG	1	0.28735632183908044	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAATAGATCGATAGAGCACAACTGA	1	0.28735632183908044	No Hit
ATTCCTAAGGATAAGATGAAGACAAGATCGGAAGAGCACACGTCTGAACC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TGCGGTCTCGTCGGCTCGAGAGCGAGAACGCAACAGCACACGTCTGAACT	1	0.28735632183908044	No Hit
AAATGATTGAGCAAAGGCGAAGCTGAGCCCCAGATCGGAAGAGCACACGT	1	0.28735632183908044	No Hit
AAAATTGGCTAAGAGTTTTAGAATAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGAAGGGGCATGCAGAGGAGAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTTGCGCTTATAAAAGAAAGGACTAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGACTGTTGTAGCTGTAATCTAAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACACGACTCTCGGCAACGGATATCTGAGCAGCTCGCAACTGCACTCCT	1	0.28735632183908044	No Hit
AGTCCGAGAGAAGGGAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1	0.28735632183908044	No Hit
ATTGTTGCCGAGCTGTGTGCTACAGATCGGAAGAGCACACGTCTGAACTC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGGAGGTAGGGTCAAGCGGCCGAGATCGGAAGAGCACACGTCTGAACTC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCTGGGCCAGACAACCAAACATAAGATCGGAAGAGCACACGTCTGAACTC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGACCCCAGGTCAGTCGGGACTACCCGCTGAGTTTAAGCAGATCGGAAG	1	0.28735632183908044	No Hit
TTCTCCGGTTTTGTGGCTCTTAAAAGATGGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCTTCAGATCGGGAGAGCAC	1	0.28735632183908044	No Hit
GTCTTGGAAGTCGAAATCCGCTAAGGAGTAGATCGGAAGAGCACACGTCT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACACGACTCTCGGCAACAGATCGGAAGAGCACACGTCTGAACTCCAGT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGCGACGACGACTTCTGCTCCGGAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAATCAGATAAGAACAGAACACGTC	1	0.28735632183908044	No Hit
AGCGGAGGAGAAGAAACTTAGATCGGAAGAGCACACGTCTGAACTCCAGT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTAGATCGGAAGAGC	1	0.28735632183908044	No Hit
TCCGTTGTAGTCTAGCTGGTAAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACACGACTCTCGGCAACGGATATCTCGGCTCTAGAAAGGAAGAGCACA	1	0.28735632183908044	No Hit
TTAAGGATCAAATCGGATTAGGGTAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGCAACATTCGGCCGTAGAGATGAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGCCAAGGATGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCCCAGGTCAGTCGGGACTACCCGCAAGATCGGAAGAGCACACGTC	1	0.28735632183908044	No Hit
CGGGGAGGTAGTGAGATCGGAAGAGCACACTCTGAACTCCAGTCACACGA	1	0.28735632183908044	No Hit
GGTACAGACCCGGGTTCGATTCCCGGCTGGTGCACCAGATCGGATGAGCA	1	0.28735632183908044	No Hit
CGAGCACCTCTGAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTTA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGGCCGGGTCGTGCCTCCGGCGCCGTTACTTTGAAGATCGGAAGAGCAC	1	0.28735632183908044	No Hit
CGGTATACCTTTTGATCCACGGACTAGATCGGAAGAGCACACGTCTGAAC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACACGACTCTCGGCAACGGATATCTCGAGATCGGAAGAGCACACGTCTGA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGCCTGCGAGCTCCCCATCCGAGATCGGAAGGGCACACGTCTGAACTCC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
AGTTTGTTTGATGGTACGTGCTACTCGGAAGATCGGAAGAGCACACGTCT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCGTTTATCAACCGACTGTGATAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGCATGGTTGATGATTGTTAGAAGATCGGAAGAACACACGTCTGAACTC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGATGATCTTTCAAAAACTTGGAGATCGGAAGAGCACACGTCTGAACTCC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCAGAATTCACCAAGTGTTGAGATCGGAAGAGCACACATCTGAACTCC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
ATAGGTGTGGACGTCCTCTTTAGGAGATCGGAAGAGCAACGTCTGAACTC	1	0.28735632183908044	No Hit
CCCTACTGATGACCGCGCCGCAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGACTCA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGATGCGATCATACCAGCACTAAAGCACACGTCTTAGATCGGAAGAGCAC	1	0.28735632183908044	No Hit
CGGTATGGTGTAGTGGCTAACATTTCGCGCTAGATCGGAAGAGCACACGT	1	0.28735632183908044	No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCAGATCGGAAGAGCACACG	1	0.28735632183908044	No Hit
ACAGTAGTAAACAAAAAGATCAAGATATTAAGCTCACACATCGAAATACA	1	0.28735632183908044	No Hit
TCCGATGTAGTCTAGTTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACACGACTCTCGGCAACGGATATCTCGGAGATCGGAAGAGCACACGTC	1	0.28735632183908044	No Hit
CGCAGATGGGACTTAGCGACGAAGATCTGAAGAGCACACGTCTGAACTCC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGGGATGTAGCTTAGATCGGAAGAGCACACGTCTAACTCCAGTCACACTT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GGGGAATCCGACTGTTAGATCGGAGGAGCACACGTCTGAACTCCAGTCAC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AACCATATCGTCTACGATGACTAGATTGGAAGAGCACACGTCTGAACTCC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GATATCTCGGCCGACACGACTCTCGGCAACGAGATCGGAAGAGCACACGT	1	0.28735632183908044	No Hit
AGCAAGTGATGATCATACTCGGATTCACCTTTAGATCGGGAGAGCACACG	1	0.28735632183908044	No Hit
GTCGTTGTAGTATAGTGGTGAGTATTCCCGCCTGCAGATCGGAAGAGCAC	1	0.28735632183908044	No Hit
ATACGCTGAAATTGTCCACTCTAGATCGGAGGAGCACACGTCTGAACTCC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CCACACGAAAATCCGCTCAGCAGATCTCTAGAAACGAATAACAAACGAAT	1	0.28735632183908044	No Hit
TTGAAGGCCGAAGAGGAGAAAGGTTCCAGATCGGAAGAGCACACGTCTGA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGACCCCAGGTCAGTCGGGAAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GACGGAGTGGTAGTGGAGGGTAAGATCGGAAGAGCACACGTCTGAACTCC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGAGGTGTAGGATAAGTGGGAGCCTCCAGATCGGAAGAGCACACGTCT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTGAAGCGATCTCGTACTAAACGAAAGGTCGGAAGAGCACACGTCTGA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CAAATGAGAACTTTGAAAGATCGGAAGAGCACACGTCTGAACTCCATTCA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
AAATTATTAAAACAAATGTAGAAGATCAGAAGACCACACGTCACAAAACA	1	0.28735632183908044	No Hit
GGTGGCTGTAGTTTAGTGGTAAGAATTCTACGAGATCGGAAGAGCACACG	1	0.28735632183908044	No Hit
CGCGCTACACTGATGTATCCAAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATAGCAGTCGGCGTGTGAATCATTAGCCATGCGACCCTCGGCAGCAAGAA	1	0.28735632183908044	No Hit
AAAGGATTGGCTCTGAGGGTTGGGCAGATCGGAAGAGCACACGTCTGAAC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGACTGAAGGGTGCTCCCTGGAGAGGGAGCCTAGATCGGAAGAGCACA	1	0.28735632183908044	No Hit
TTGAGCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGAAAACGCAC	1	0.28735632183908044	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCTAGATCGGAAGAGCA	1	0.28735632183908044	No Hit
CACTGTTGGATTCTCTATATATAGAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TACCTGGATGATCCTGCCAGTAGTCATATGCTTGTCTAGATCGGAAGAGC	1	0.28735632183908044	No Hit
CACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACTCC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACAGAAGAGAGTGAGCACAGATCGGAAGAGCACACGTCTGAACTCCAG	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCGGTTTCGGACAGCGAGGGAAGAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACCGGCTCGACCAAGATCGGAAGAGCACACGTCTGAACTCCGTCACACG	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TAGGGATTTTTCGAGTTGATTCGTTAGATCGGAAGAGCACACGTCTGAAC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAGATCGGGAGAGCA	1	0.28735632183908044	No Hit
GTAAAAATTCTGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCGCA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AGCAAGGATTGACAGACTGAAGATCGGAAGAGCACACGTCTGAACTCCAG	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGGAACGGCTCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCG	1	0.28735632183908044	TruSeq Adapter, Index 10 (97% over 36bp)
TCTCGCATCGATGAAGAACGTAAGATCGGAAGAGCACACGTCTGAACTCC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGATACCTGGTGTGAATTGCAGAATCCCAGATCGGAAGAGCACACGTCT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCAGAACTGGCGATGCGGGATGAACCAGATCGGAAGAGCACACGTCTGA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAACTAATTCGAACTGAGATCGGAAGAGCACACGTCTGAACTCCGTCAC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CTAAACTTGATTGATCTGGCCGGTTCAGAAAGACTGTCGAGATCGGAAGA	1	0.28735632183908044	No Hit
AATCTGTTGGGCTACAGATCGGAAGAGCACACGTCTGAACTCCATCACAC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTGTAGATCGGAGGAGCA	1	0.28735632183908044	No Hit
GTTATCCAAGCCGCTCACCTTATGAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTACATGCAGAGCCTTGGCGCCGCAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGATCACCCATGACTTCTGTGTAGATCGGAAGAGCACACGTCTGAACTCC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACGACTCTCGGCAACGGATATCTCGGCTCTCAGATCGGAAGAGCACACG	1	0.28735632183908044	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	1	0.28735632183908044	No Hit
GCACGTCTGCCTGGGCGTCACGCCAGATCGGAAGGGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCTTCCAGATCGGAAGAGAA	1	0.28735632183908044	No Hit
TCCTGGGAAGTCCTCGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCGTTGTAGTCTAGCAGATCGGTATAACACACGTCTGAACACCAGTCTC	1	0.28735632183908044	No Hit
TCCACTGAGATCCAGCCCCGCGTCGCAAGATCGGAAGAGCACACGTCTGA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGCCCCGCGTCGCACGGATTCGTAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGCAATATCGTCGGTCCTTATACAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGGACTAACTGTGACTGATGAAGAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTTCATCGTGATGGGGATAGATCAAGATCGGAAGAGCACACGTCTGAAC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTGAGATCGGAAGAGCAC	1	0.28735632183908044	No Hit
CCGACTTTGTGAAATGACTAGATCGGAAGAGCGCACGTCTGAACTCCAGT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GCGACGTTGCTTTTTGATCCTTCGATGTCGGCTCTTCAGATCGGAAGAGC	1	0.28735632183908044	No Hit
ACGGAGCTTATATATAAAAAATAAAATCGGAAGAGCACACGTCTGAACTC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CGACACGACTCTCGGCAACAGATCGGAAGAGCACACGTCTCAACTCCAGT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GCAACGTCCTCTAAACGATCCTCTAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGTCGGGACTACCCGCTGAGTTTAAGCGGATCGGAAGAGCACACGTCTGA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ATAGACATGTGTGTGCGAGCCAAGATCGGAAGAGCACACGTCTGAACTCC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCGTGCCACGGAATCGGGAGCTCCAAGTGGGCCAAGATCGGAAGAGCAC	1	0.28735632183908044	No Hit
AGTGGCCGATCGACGATTTTTGTAAGATCGGAAGAGCACACGTCTGAATC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AAATTTCATCGTGATGGGGATAGATCAAGATCGGAAGAGCACACGTCTGT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ATTTCTAGGTGCTAAAGTTTACTGAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGTTAGTTCTCCTAGGGTTAGGATATTACGCTACACATGCGAACAGCAC	1	0.28735632183908044	No Hit
CGGCGAGAACCGACCGGCTCGACCCTTCTGCCGGCGAGATCGGAAGAGCA	1	0.28735632183908044	No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCTTCAGATCGGAAGGGCAC	1	0.28735632183908044	No Hit
GAAATATTGTAAACTGAACTTAGTACTAGGATTGTCGATAGAGGAATCGA	1	0.28735632183908044	No Hit
GTGGCTGTAGTTTAGTGGTAAGAATTCTAAGATCGGAAGAGCACACGTCT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGACCCCGCTGGCACCTTATGAGAAATCAGATCGGAGAGCACACGTCT	1	0.28735632183908044	No Hit
TTATTTCCGACGGTACTCTAGGAAAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGGCCTAATCATAGAGAAAACGTAGATCGGAAGAGCGCACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CACGACTCTCGGCAACGGAAAACTAGAGAACGGAAGAGCACACCACTGAA	1	0.28735632183908044	No Hit
TCGGTTCAGCCGGAGGTAGGGTCCAGAGATCGGAAGGCACACGTCTGAAC	1	0.28735632183908044	No Hit
CCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCAGATCGGAAGAGCAC	1	0.28735632183908044	No Hit
TCTCGGCTCTCGCATCGATGAAGAACGTAAGATCGGAAGAGCACACGTCT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGTAAAAGCTCGTTTGATTATGATTTCCAGTAGATCGGAAGGGCACAC	1	0.28735632183908044	No Hit
GAAGACATATACGCGTATCTGAGCTTGAGATCGGAAGAGCACACGATGAA	1	0.28735632183908044	No Hit
CGATAGGTAGTGTCGTGCCTGGGTAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGGAAGGAGAACTCGTAAGATCGGAAGAGCACACGACTCAACTCCAGAC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTCTGGTGGAGGCTCGAAGATCGGAAGAGCACACGTCTGAACTCCATCAC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
CTTGAGAGGTGTAGGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCAGATAGGAAAAGCAC	1	0.28735632183908044	No Hit
GAGGAAGGAGAAGTCGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
AGAACTCCGAAGTTAAGCGTGCTAGATCGGAAGAGCACACGTCTGAACTC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGTGGAGACCTGGGCTAGATCGGAAGGGCACACGTCTGAACTCCAGTC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ATACTCTGATGATTTATGACATTTAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCT	1	0.28735632183908044	TruSeq Adapter, Index 8 (97% over 36bp)
CGAACTGTGAAACTGCGAATGGCTCATTAAATCAGTCAGATCGGAAGAGC	1	0.28735632183908044	No Hit
GCTACACGGGTGCTCAGAATCGAAGATCAGAAGAGCACACGTCTGAACTC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GCCGTTACTTTGAAGAAATTAGAAGATCGGAAGAGCACACGTCTGAACTC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGGACCAGGCTTCATTCCCCAGATCGGAAGAGCACACGTCTGAACTCCG	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
ATTGGGGAACAGAGAGGTTATGCCAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTGATTTTTTGGAGCGGAATGGTAGATCGGAAGAGCACACGTCTAAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TCAAAGAATAGGCCCGGATCGGCTAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATGGAACAATGTAGGCAAGGGAAGTCGGCAAGATCGGAAGAGCACACGT	1	0.28735632183908044	No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATATTAGAAGGGAAGTCGGCAGGTCT	1	0.28735632183908044	No Hit
CCAGCCCCGCGTCGCACGGATTCGTAGATCGGAAGAGCACACGTCTGAAC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGGTGTAGGATAAGTGGGAGCCCAGATCGGAAGAGCACACGTCTGATC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TGAGATCGGCACCAGGAATGGTTAGATCGGAAGAGCACACGTCTGAACTC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCAATGATGATTAGCTATTACATCAGATCGGAAGGGCACACGACTGAA	1	0.28735632183908044	No Hit
AACGATGAAGAAAAGCTCCGTGACGAGATCGGAAGAGCACACGTCTGAAC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGCGTGGAGCCTGCGGCTTAAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTAACACATGCAAATCTAACGAAGTGCTCCTGGACGAGATCGGAAGAGCA	1	0.28735632183908044	No Hit
AAAGTTGCATTGCTTTCAAAAAAAAAAAAAAAAAAAACAAAAAACAAAAC	1	0.28735632183908044	No Hit
CGACACGACTCTCGGCAAGATCGGAAGAGCACACGTCTAAACTCCAGTCA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GTGCTCTCCAATGAACAAACTGAGATCGGAAGAGCACACGTCTGAACTCC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGTTGTAGTCTAGATGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTTGTAGTCTAGCAGATCGGATGAGCACACGTCTGAACTCCAGTCAC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TCCCCGCACCTCCAGATCGGAAGAGCACACTCTGAACTCCAGTCACACTT	1	0.28735632183908044	TruSeq Adapter, Index 8 (95% over 21bp)
GGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATAGATCGGAAGAGCACG	1	0.28735632183908044	No Hit
GGGACGTGGCGGCAGACGGAGATCGGAAGAGCACACGTCGAACTCCAGTC	1	0.28735632183908044	No Hit
ACGGGGCATTGTAAGTGGCAGAGTATATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CGGGCCCAGAGCCACATTTAAGCAGACCACCATCTTTAGATCGGAAAAGC	1	0.28735632183908044	No Hit
GAGGCGAAAGAAGCTGTAGAGAACAGATCGGAAGAGCACACGTCTGAATT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CGAGCACCTCTGAGCATAGATCGGAAGAGCACACGTCTGAACACCGTCAC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GAGAGTGTTGTGCTAGGTACGGCAGATCGGAAGAGCACACGTCTGACTCC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCGGCCTCTAGAATTTTTTTCTTGAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACAACCCACTGATGTAGCACAATCAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTTGACTCTAGTCCGACTTTGTGAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGTAAGGATACCTGAGAGGATTAGATCGGAAGAGCACACGTCTGAACTC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGACAAGGGGAATCCGACTGTAGATGGGAAGAGCACACGTCTGAACTCC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
AAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCAGATCGGAAG	1	0.28735632183908044	No Hit
AATACTGCGGCGTGTTGCCAGAGCAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAATGACACTGCCGTAGACGAAAGATCGGAAGAGCACACGTCTGAACTCC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATTCTTGGATTTATGAAAGACGAACAACTGCGAAGATCGGAAGAGCACA	1	0.28735632183908044	No Hit
TGCCACGTCAAATCCACGTAGCCGGAGATCGGAAGAGCACACGTCTGAAC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACACGACTCTCGGCAACGAGATGGGAAGAGCACACGTCTGAACTCCAGTC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GGAAGGACGTCAATAGACACACAGTCCTTGACGGTATAATAACCACCATC	1	0.28735632183908044	No Hit
GGGACGGTCGCGTTTTCGGCGTGTAGATCGGAAGAGCACACGTTGAACTC	1	0.28735632183908044	No Hit
CGGCGAGAACCGACCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
CGGGGATAGCTCAGAGATCGGAAGAGCAAACGTCTGAACTCCAGTCACAC	1	0.28735632183908044	TruSeq Adapter, Index 5 (97% over 35bp)
CTCGAAGCTCCCTTCCCAACGGGCGGCGTGCTGAATAGATCGGAAGAGCA	1	0.28735632183908044	No Hit
ACACGACACTCTGCAAAAGATCGGAAGACCACACGTCTGAATCCAGTCAC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GCCTGGGCGTCACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTAAC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
ACTGACGGCCGTCAGTTGTAGACGAGATTGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AATTCTTGGATTTATGAAAGACGAACAAGATGGGAAGAGCACACGTCTGA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AAGACGGTCTTCTAATAAATTCGGAGATCGGAAGAGCACACGGCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CAGGTATCTTCGGACTCGTATCAGAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCCATTCTGTGTTCAAAAAAAAAAAAAAAAAAAAAGGAACGGAAGAACA	1	0.28735632183908044	No Hit
ATCATATACACTGGCGGCTGTATAAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAAATACGAACAGGACAACTAAGA	1	0.28735632183908044	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAGGTAGGAAAGGGACACGGAATCA	1	0.28735632183908044	No Hit
GCTCGGGGGTCCCGGCCCCGAACCCGTCGGCTAGATCGGAAGAGCACACG	1	0.28735632183908044	No Hit
GCCGTTACTTTGAAGAAGATCGGAAGAGCACATGTCTGAACTCCAGTCAC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CACGGGGAGGTAGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCGC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCTAGATCGGAAGTGCA	1	0.28735632183908044	No Hit
CGTCGTCGGCGCGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTTA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GGACCACCTCTGTATCGAACTACTAGATCGGAAGAGCACCGTCTGAACTC	1	0.28735632183908044	No Hit
GACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAGATCGGAA	1	0.28735632183908044	No Hit
TAAAACCCGTCGGGTATTCGGAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTACCAGTTAGAAGACCAATAGACAGATCGAAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ACCGACCGGCTCGACCCAGATCGGAAGAGCACACGTCTGAACTCCAGTAA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GCACCAGTGGTCTAGTGGTAGAATAGTACCCAGATCGGAAGTGCACACGT	1	0.28735632183908044	No Hit
CGTTATTTTACTTCCGTGGGTCGGAAGCGGGGCACCAGATCGGAAGAGCA	1	0.28735632183908044	No Hit
TGTAGGATAAGTGGGAGCTAGATCGGAAGGGCACACGTCTGAACTCCAGT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TTACGAATAAATAGGATATAGAATAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGGGCGGAGCGGCCGTCGGTGCAGATCTTGGTGAGATCGGAAGAGCAC	1	0.28735632183908044	No Hit
GAGATGCTTCGAGTCGGAGAGGTGAGATCGGAAGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTGGAGAACTGAGATCATCTCAGATCGGAAGAGCACACGTCTGAACTCC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCAGGTCCAGACATAGCAAGGATTGACAGATCGGAAGGGCACACGTCTGA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CGACACGACTCTCGGCAACGGATATCTCGGCTAGATCGGTAGAGCACACG	1	0.28735632183908044	No Hit
AAATCTCACTATCCAAACAAACTAGATCGGAAGAGCACACGTCTGAACTA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TTTGAATAGCACCGGACACGACATAGATCGGAGGAGCACACGTCTGAACT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TCCTCAGTAGCTCAGTGGTAGAGCGGTCGGCTGAGATCGGAAGAGCACAC	1	0.28735632183908044	No Hit
CGGCTCGGGGCGTGGACCGAGATCGGAGGAGCACACGTCTGAACTCCAGT	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TGACGTGGTTACAAGAAACAAAAGATCGGAAGAGCACACGTCTGAACTCC	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGGCTCAGAACCTCACTAAGCAAACCACCATCTTAGATCGAAGAGCACA	1	0.28735632183908044	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAGAACGGAAGAGCACACGTCTGAA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GACCGGCTCGACCAGATCGGAAGAGCACACGTCTGAACTCCGTCACACGA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
ATCTAGTGATGTATTAATCAGTCTAGATCGGAAGAGCACACTCTGAACTC	1	0.28735632183908044	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAATAGCGCGGAAGAGCACACCTCA	1	0.28735632183908044	No Hit
TCAGTTGGTAGAGCAGAAAGATCGGAAGAGCACACGTCTGAACTCCATCA	1	0.28735632183908044	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AACACCCCCTTAGTCGTTTAGATGAGATCGGAAGAGCACACGTCCTGAAC	1	0.28735632183908044	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.28735632183908044	0.0	0.0	0.0	0.0
11	0.28735632183908044	0.0	0.0	0.0	0.0
12	0.28735632183908044	0.0	0.0	0.0	0.0
13	0.28735632183908044	0.0	0.0	0.0	0.0
14	2.586206896551724	0.0	0.0	0.0	0.0
15	3.4482758620689653	0.0	0.0	0.0	0.0
16	4.310344827586207	0.0	0.0	0.0	0.0
17	5.747126436781609	0.0	0.0	0.0	0.0
18	8.620689655172415	0.0	0.0	0.0	0.0
19	10.057471264367816	0.0	0.0	0.0	0.0
20	11.781609195402298	0.0	0.0	0.0	0.0
21	12.35632183908046	0.0	0.0	0.0	0.0
22	14.942528735632184	0.0	0.0	0.0	0.0
23	19.25287356321839	0.0	0.0	0.0	0.0
24	22.413793103448278	0.0	0.0	0.0	0.0
25	37.93103448275862	0.0	0.0	0.0	0.0
26	40.51724137931034	0.0	0.0	0.0	0.0
27	41.0919540229885	0.0	0.0	0.0	0.0
28	53.44827586206897	0.0	0.0	0.0	0.0
29	54.59770114942529	0.0	0.0	0.0	0.0
30	58.04597701149425	0.0	0.0	0.0	0.0
31	59.195402298850574	0.0	0.0	0.0	0.0
32	60.91954022988506	0.0	0.0	0.0	0.0
33	62.64367816091954	0.0	0.0	0.0	0.0
34	63.2183908045977	0.0	0.0	0.0	0.0
35	65.22988505747126	0.0	0.0	0.0	0.0
36	67.816091954023	0.0	0.0	0.0	0.0
37	70.6896551724138	0.0	0.0	0.0	0.0
38	71.83908045977012	0.0	0.0	0.0	0.0
39	71.83908045977012	0.0	0.0	0.0	0.0
40	72.41379310344827	0.0	0.0	0.0	0.0
41	72.70114942528735	0.0	0.0	0.0	0.0
42	73.27586206896552	0.0	0.0	0.0	0.0
43	73.27586206896552	0.0	0.0	0.0	0.0
44	73.5632183908046	0.0	0.0	0.0	0.0
45	73.85057471264368	0.0	0.0	0.0	0.0
46	73.85057471264368	0.0	0.0	0.0	0.0
47	73.85057471264368	0.0	0.0	0.0	0.0
48	73.85057471264368	0.0	0.0	0.0	0.0
49	73.85057471264368	0.0	0.0	0.0	0.0
50	73.85057471264368	0.0	0.0	0.0	0.0
51	73.85057471264368	0.0	0.0	0.0	0.0
52	73.85057471264368	0.0	0.0	0.0	0.0
53	73.85057471264368	0.0	0.0	0.0	0.0
54	73.85057471264368	0.0	0.0	0.0	0.0
55	73.85057471264368	0.0	0.0	0.0	0.0
56	73.85057471264368	0.0	0.0	0.0	0.0
57	73.85057471264368	0.0	0.0	0.0	0.0
58	73.85057471264368	0.0	0.0	0.0	0.0
59	73.85057471264368	0.0	0.0	0.0	0.0
60	73.85057471264368	0.0	0.0	0.0	0.0
61	73.85057471264368	0.0	0.0	0.0	0.0
62	73.85057471264368	0.0	0.0	0.0	0.0
63	73.85057471264368	0.0	0.0	0.0	0.0
64	73.85057471264368	0.0	0.0	0.0	0.0
65	73.85057471264368	0.0	0.0	0.0	0.0
66	73.85057471264368	0.0	0.0	0.0	0.0
67	73.85057471264368	0.0	0.0	0.0	0.0
68	73.85057471264368	0.0	0.0	0.0	0.0
69	73.85057471264368	0.0	0.0	0.0	0.0
70	73.85057471264368	0.0	0.0	0.0	0.0
71	73.85057471264368	0.0	0.0	0.0	0.0
72	73.85057471264368	0.0	0.0	0.0	0.0
73	73.85057471264368	0.0	0.0	0.0	0.0
74	73.85057471264368	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Rejected 17 READS because READLEN < 1
Read 17 spots for SRR11699360.sra
Rejected 17 READS because READLEN < 1
Read 17 spots for SRR11699360.sra
Rejected 17 READS because READLEN < 1
Read 17 spots for SRR11699360.sra
Rejected 17 READS because READLEN < 1
Read 17 spots for SRR11699360.sra
Rejected 17 READS because READLEN < 1
Read 17 spots for SRR11699360.sra
Rejected 17 READS because READLEN < 1
Read 17 spots for SRR11699360.sra
Rejected 17 READS because READLEN < 1
Read 17 spots for SRR11699360.sra
Rejected 17 READS because READLEN < 1
Read 17 spots for SRR11699360.sra
Written 17 spots for SRR11699360.sra
Rejected 17 READS because READLEN < 1
Read 17 spots for SRR11699360.sra
Rejected 17 READS because READLEN < 1
Read 17 spots for SRR11699360.sra
Rejected 17 READS because READLEN < 1
Read 17 spots for SRR11699360.sra
Rejected 17 READS because READLEN < 1
Read 17 spots for SRR11699360.sra
Rejected 17 READS because READLEN < 1
Read 17 spots for SRR11699360.sra
Rejected 17 READS because READLEN < 1
Read 17 spots for SRR11699360.sra
Rejected 17 READS because READLEN < 1
Read 17 spots for SRR11699360.sra
Rejected 17 READS because READLEN < 1
Rejected 17 READS because READLEN < 1
Read 17 spots for SRR11699360.sra
Rejected 17 READS because READLEN < 1
Read 17 spots for SRR11699360.sra
Rejected 17 READS because READLEN < 1
Read 17 spots for SRR11699360.sra
Rejected 25 READS because READLEN < 1
Read 25 spots for SRR11699360.sra
Written 17 spots for SRR11699360.sra
Written 17 spots for SRR11699360.sra
Written 17 spots for SRR11699360.sra
Written 17 spots for SRR11699360.sra
Written 17 spots for SRR11699360.sra
Written 17 spots for SRR11699360.sra
Written 17 spots for SRR11699360.sra
Written 17 spots for SRR11699360.sra
Written 17 spots for SRR11699360.sra
Written 17 spots for SRR11699360.sra
Written 17 spots for SRR11699360.sra
Written 17 spots for SRR11699360.sra
Written 17 spots for SRR11699360.sra
Written 17 spots for SRR11699360.sra
Read 17 spots for SRR11699360.sra
Written 17 spots for SRR11699360.sra
Written 17 spots for SRR11699360.sra
Written 17 spots for SRR11699360.sra
Written 25 spots for SRR11699360.sra
Written 17 spots for SRR11699360.sra
SRR ids: ['SRR11699360.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_2y8vscnh
SRR11699360.sra spots: 348
blocks: [[1, 17], [18, 34], [35, 51], [52, 68], [69, 85], [86, 102], [103, 119], [120, 136], [137, 153], [154, 170], [171, 187], [188, 204], [205, 221], [222, 238], [239, 255], [256, 272], [273, 289], [290, 306], [307, 323], [324, 348]]
SRR11699360 file size 71
SRR11699360 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR11699360 SRR11699360_1.fastq
Input file:	SRR11699360_1.fastq
trimmed:	SRR11699360-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	20
Wed Jun 18 20:09:26 2025 >> started

Wed Jun 18 20:09:26 2025 >> done (0.355s)
348 reads processed; of these:
 35 (10.06%) short reads filtered out after trimming by size control
  0 ( 0.00%) empty reads filtered out after trimming by size control
313 (89.94%) reads available; of these:
281 (89.78%) trimmed reads available after processing
 32 (10.22%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  8	  2.56%
 19	  8	  2.56%
 20	  2	  0.64%
 21	 10	  3.19%
 22	 21	  6.71%
 23	 14	  4.47%
 24	 67	 21.41%
 25	  9	  2.88%
 26	  6	  1.92%
 27	 46	 14.70%
 28	  5	  1.60%
 29	 12	  3.83%
 30	  5	  1.60%
 31	  7	  2.24%
 32	  8	  2.56%
 33	  3	  0.96%
 34	  8	  2.56%
 35	 12	  3.83%
 36	 14	  4.47%
 37	  5	  1.60%
 38	  1	  0.32%
 39	  2	  0.64%
 40	  2	  0.64%
 41	  3	  0.96%
 42	  0	  0.00%
 43	  1	  0.32%
 44	  1	  0.32%
 45	  0	  0.00%
 46	  0	  0.00%
 47	  0	  0.00%
 48	  1	  0.32%
 49	  0	  0.00%
 50	  0	  0.00%
 51	  0	  0.00%
 52	  0	  0.00%
 53	  0	  0.00%
 54	  0	  0.00%
 55	  0	  0.00%
 56	  0	  0.00%
 57	  0	  0.00%
 58	  0	  0.00%
 59	  0	  0.00%
 60	  0	  0.00%
 61	  0	  0.00%
 62	  0	  0.00%
 63	  0	  0.00%
 64	  0	  0.00%
 65	  0	  0.00%
 66	  0	  0.00%
 67	  0	  0.00%
 68	  0	  0.00%
 69	  0	  0.00%
 70	  0	  0.00%
 71	  0	  0.00%
 72	  0	  0.00%
 73	  0	  0.00%
 74	  0	  0.00%
 75	  0	  0.00%
 76	  0	  0.00%
 77	  0	  0.00%
 78	  0	  0.00%
 79	  0	  0.00%
 80	  0	  0.00%
 81	  0	  0.00%
 82	  0	  0.00%
 83	  0	  0.00%
 84	  0	  0.00%
 85	  0	  0.00%
 86	 32	 10.22%
313 reads passed initial QC
                                 Started job on |	Jun 18 20:16:01
                             Started mapping on |	Jun 18 20:17:03
                                    Finished on |	Jun 18 20:17:13
       Mapping speed, Million of reads per hour |	0.11

                          Number of input reads |	313
                      Average input read length |	33
                                    UNIQUE READS:
                   Uniquely mapped reads number |	40
                        Uniquely mapped reads % |	12.78%
                          Average mapped length |	23.88
                       Number of splices: Total |	0
            Number of splices: Annotated (sjdb) |	0
                       Number of splices: GT/AG |	0
                       Number of splices: GC/AG |	0
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	0
                      Mismatch rate per base, % |	0.63%
                         Deletion rate per base |	0.00%
                        Deletion average length |	0.00
                        Insertion rate per base |	0.00%
                       Insertion average length |	0.00
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	62
             % of reads mapped to multiple loci |	19.81%
        Number of reads mapped to too many loci |	51
             % of reads mapped to too many loci |	16.29%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	16.61%
                     % of reads unmapped: other |	34.50%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	211	211	211
N_multimapping	62	62	62
N_noFeature	38	39	39
N_ambiguous	0	0	0
UnstrandedReadsAssigned:2 PositiveStrandReadsAssigned:1 NegativeStrandReadsAssigned:1
Dataset is classified unstranded
MeadianReadLen=27 20thPercentileLength=23 echo kmer=19
SRR11699360 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 72,539
[index] number of k-mers: 56,563,136
[index] number of equivalence classes: 414,827
[quant] running in single-end mode
[quant] will process file 1: SRR11699360-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 313 reads, 105 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 302 rounds

  72540 SRR11699360.ke.tsv
  44303 SRR11699360.se.tsv
 116843 total
==> SRR11699360.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Zm00001eb096110_T001	2067	1968	0	0
Zm00001eb321680_T001	3417	3318	0	0
Zm00001eb323640_T001	3273	3174	0	0
Zm00001eb314140_T001	893	794	0	0
Zm00001eb080260_T001	3529	3430	0	0
Zm00001eb026490_T001	2433	2334	0	0
Zm00001eb026490_T002	2229	2130	0	0
Zm00001eb026490_T003	2220	2121	0	0
Zm00001eb281360_T001	2412	2313	0	0

==> SRR11699360.se.tsv <==
Zm00001eb015280	0
Zm00001eb000610	0
Zm00001eb033210	0
Zm00001eb044610	0
Zm00001eb014360	0
Zm00001eb051320	0
Zm00001eb021860	0
Zm00001eb011690	0
Zm00001eb016840	0
Zm00001eb052060	0
SRR11699360 completed mapping pipeline successfully
