Starting /dee2/code/volunteer_pipeline.sh SRR11699580
    current disk space = 2457395515392
    free memory = 1580255760 
SRR11699580 SRAfilesize
8cf450563caf03e3fd5ee3bb6b3acf98  SRR11699580.sra
SRR11699580.sra file validated
SRR11699580 is single end
SRR11699580 is conventional basespace
SRR11699580 read1 length is 86 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR11699580_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	311
Sequences flagged as poor quality	0
Sequence length	86
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.244372990353696	32.0	32.0	32.0	32.0	32.0
2	31.186495176848876	32.0	32.0	32.0	32.0	32.0
3	31.627009646302252	32.0	32.0	32.0	32.0	32.0
4	31.360128617363344	32.0	32.0	32.0	32.0	32.0
5	31.511254019292604	32.0	32.0	32.0	32.0	32.0
6	34.47909967845659	36.0	36.0	36.0	32.0	36.0
7	34.630225080385856	36.0	36.0	36.0	32.0	36.0
8	34.938906752411576	36.0	36.0	36.0	36.0	36.0
9	34.71704180064309	36.0	36.0	36.0	32.0	36.0
10-11	34.762057877813504	36.0	36.0	36.0	34.0	36.0
12-13	34.964630225080384	36.0	36.0	36.0	34.0	36.0
14-15	34.79903536977492	36.0	36.0	36.0	32.0	36.0
16-17	34.78938906752411	36.0	36.0	36.0	34.0	36.0
18-19	34.937299035369776	36.0	36.0	36.0	34.0	36.0
20-21	35.10610932475885	36.0	36.0	36.0	34.0	36.0
22-23	34.725080385852095	36.0	36.0	36.0	34.0	36.0
24-25	34.659163987138264	36.0	36.0	36.0	34.0	36.0
26-27	34.32475884244373	36.0	36.0	36.0	32.0	36.0
28-29	34.2508038585209	36.0	36.0	36.0	32.0	36.0
30-31	33.88745980707395	36.0	36.0	36.0	29.5	36.0
32-33	34.11736334405145	36.0	36.0	36.0	32.0	36.0
34-35	34.29581993569132	36.0	36.0	36.0	32.0	36.0
36-37	34.323151125401935	36.0	36.0	36.0	32.0	36.0
38-39	33.954983922829584	36.0	36.0	36.0	29.5	36.0
40-41	33.9903536977492	36.0	36.0	36.0	32.0	36.0
42-43	33.7508038585209	36.0	36.0	36.0	27.0	36.0
44-45	34.159163987138264	36.0	36.0	36.0	32.0	36.0
46-47	33.77170418006431	36.0	36.0	36.0	24.0	36.0
48-49	33.69131832797427	36.0	36.0	36.0	24.0	36.0
50-51	33.38585209003215	36.0	36.0	36.0	17.5	36.0
52-53	32.934083601286176	36.0	36.0	36.0	14.0	36.0
54-55	33.71543408360129	36.0	36.0	36.0	26.5	36.0
56-57	32.79742765273312	36.0	34.0	36.0	14.0	36.0
58-59	32.13183279742765	36.0	34.0	36.0	14.0	36.0
60-61	32.2459807073955	36.0	32.0	36.0	14.0	36.0
62-63	32.40675241157557	36.0	34.0	36.0	14.0	36.0
64-65	32.10771704180064	36.0	32.0	36.0	14.0	36.0
66-67	32.22508038585209	36.0	32.0	36.0	14.0	36.0
68-69	31.79421221864952	36.0	32.0	36.0	14.0	36.0
70-71	31.85209003215434	36.0	32.0	36.0	14.0	36.0
72-73	31.580385852090032	36.0	32.0	36.0	14.0	36.0
74-75	31.639871382636656	36.0	32.0	36.0	14.0	36.0
76-77	32.10932475884244	36.0	32.0	36.0	14.0	36.0
78-79	32.58199356913184	36.0	34.0	36.0	14.0	36.0
80-81	32.657556270096464	36.0	36.0	36.0	14.0	36.0
82-83	32.61414790996784	36.0	36.0	36.0	14.0	36.0
84-85	32.68810289389067	36.0	36.0	36.0	14.0	36.0
86	32.28617363344051	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	4.0
23	5.0
24	4.0
25	8.0
26	5.0
27	6.0
28	3.0
29	5.0
30	7.0
31	12.0
32	20.0
33	21.0
34	64.0
35	145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.437299035369776	22.508038585209004	28.617363344051448	24.437299035369776
2	24.758842443729904	23.47266881028939	22.186495176848876	29.581993569131832
3	27.974276527331188	27.974276527331188	18.327974276527332	25.723472668810288
4	32.475884244372985	31.511254019292608	18.006430868167204	18.006430868167204
5	18.327974276527332	26.688102893890676	25.080385852090032	29.903536977491964
6	22.829581993569132	24.758842443729904	30.54662379421222	21.864951768488748
7	38.58520900321543	27.974276527331188	15.434083601286176	18.006430868167204
8	23.79421221864952	22.829581993569132	37.942122186495176	15.434083601286176
9	23.79421221864952	34.08360128617363	20.578778135048232	21.54340836012862
10-11	35.36977491961415	21.221864951768488	22.990353697749196	20.418006430868168
12-13	23.311897106109324	21.70418006430868	26.04501607717042	28.938906752411576
14-15	29.581993569131832	37.942122186495176	16.237942122186492	16.237942122186492
16-17	25.241157556270092	22.347266881028936	31.9935691318328	20.418006430868168
18-19	43.08681672025724	19.45337620578778	22.829581993569132	14.630225080385854
20-21	22.990353697749196	33.92282958199357	24.115755627009648	18.971061093247588
22-23	34.08360128617363	36.01286173633441	14.951768488745982	14.951768488745982
24-25	31.832797427652732	38.90675241157556	13.504823151125404	15.755627009646304
26-27	27.974276527331188	30.86816720257235	19.614147909967848	21.54340836012862
28-29	34.88745980707396	26.848874598070736	13.826366559485532	24.437299035369776
30-31	33.11897106109325	37.78135048231511	7.717041800643088	21.382636655948552
32-33	29.099678456591636	38.42443729903537	13.344051446945338	19.131832797427652
34-35	34.88745980707396	28.29581993569132	14.630225080385854	22.186495176848876
36-37	27.974276527331188	35.69131832797427	10.932475884244374	25.401929260450164
38-39	23.311897106109324	35.530546623794216	12.057877813504824	29.099678456591636
40-41	27.170418006430868	30.064308681672024	14.308681672025724	28.45659163987138
42-43	24.59807073954984	27.331189710610932	20.257234726688104	27.813504823151124
44-45	22.02572347266881	32.79742765273312	15.112540192926044	30.064308681672024
46-47	20.90032154340836	32.31511254019293	13.987138263665594	32.79742765273312
48-49	14.630225080385854	26.688102893890676	20.739549839228296	37.942122186495176
50-51	15.755627009646304	29.260450160771708	18.810289389067524	36.17363344051447
52-53	17.041800643086816	29.903536977491964	17.845659163987136	35.20900321543408
54-55	13.665594855305466	30.064308681672024	18.971061093247588	37.29903536977492
56-57	13.826366559485532	29.581993569131832	19.774919614147908	36.81672025723473
58-59	14.308681672025724	33.92282958199357	16.077170418006432	35.69131832797427
60-61	13.02250803858521	28.938906752411576	22.508038585209004	35.530546623794216
62-63	13.665594855305466	34.08360128617363	18.327974276527332	33.92282958199357
64-65	13.504823151125404	26.848874598070736	25.723472668810288	33.92282958199357
66-67	18.167202572347264	22.02572347266881	27.170418006430868	32.636655948553056
68-69	16.720257234726688	22.990353697749196	27.813504823151124	32.475884244372985
70-71	18.971061093247588	22.02572347266881	31.189710610932476	27.813504823151124
72-73	21.221864951768488	16.559485530546624	29.260450160771708	32.958199356913184
74-75	20.578778135048232	12.057877813504824	31.672025723472668	35.69131832797427
76-77	18.64951768488746	12.540192926045016	39.38906752411576	29.421221864951768
78-79	18.006430868167204	11.57556270096463	40.836012861736336	29.581993569131832
80-81	18.167202572347264	14.630225080385854	36.01286173633441	31.189710610932476
82-83	20.096463022508036	21.061093247588424	36.4951768488746	22.347266881028936
84-85	19.131832797427652	25.723472668810288	33.27974276527331	21.864951768488748
86	19.614147909967848	33.11897106109325	28.617363344051448	18.64951768488746
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	0.5
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.5
23	0.5
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.5
35	0.5
36	0.0
37	0.0
38	0.0
39	0.5
40	1.5
41	2.0
42	2.5
43	3.0
44	5.5
45	8.0
46	15.5
47	29.0
48	39.0
49	42.0
50	41.0
51	40.5
52	34.0
53	23.0
54	22.5
55	19.5
56	10.0
57	8.0
58	5.5
59	3.5
60	2.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.5
67	0.5
68	0.0
69	0.5
70	0.5
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	311.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.96141479099678
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.45454545454545	87.78135048231512
2	3.146853146853147	5.787781350482315
3	1.048951048951049	2.8938906752411575
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.34965034965034963	3.536977491961415
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCC	11	3.536977491961415	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTGAGATCGGAAGAGCAC	3	0.964630225080386	No Hit
TCCTCAGTAGCTCAGTGGTAGAGCGGTCGGCTAGATCGGAAGAGCACACG	3	0.964630225080386	No Hit
CTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCA	3	0.964630225080386	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCAGATCGGAAGAG	2	0.6430868167202572	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTCAGATCGGAAGAGCACACGTC	2	0.6430868167202572	No Hit
TCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACT	2	0.6430868167202572	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	2	0.6430868167202572	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCA	2	0.6430868167202572	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCAGCCCCGCGTCGCACGGATTCGTAGATCGGAAGAGCACACGTCTGAA	2	0.6430868167202572	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGCCATGGTGAAATTGGTAGACACGCTGCTCTTAGATCGGAAGAGCAC	2	0.6430868167202572	No Hit
ATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAAC	2	0.6430868167202572	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGGCTGTAGTTTAGTGGTTAGAATTCCACGTTGAGATCGGAAGAGCAC	2	0.6430868167202572	No Hit
CCTGCACCTGGACAGAAAGACCCTATGAAGAGATCGGAAGAGCACACGCT	1	0.3215434083601286	No Hit
TGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGT	1	0.3215434083601286	No Hit
CCGCCTCACGGCGAGAACCGACCGGCTCGACCGGGTCCCGGCCCCGAACA	1	0.3215434083601286	No Hit
ACCCGGGTTCGTTTCCCGGCTGGTACACCAAGATCGGAAGAGCACACGTC	1	0.3215434083601286	No Hit
TGTAGGATGGTCCGGCTTATTAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCCAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACGATGGATACTAGGTGCTGTGCAGATCGGAAAGAGCACACGTCTGAA	1	0.3215434083601286	No Hit
GCTGGTCCGCCGATCGGCTCGGGGCGTGGAGATCGGAAGAGCACACGTCT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGGCACTACGGTGAGACGTGTTAGATCAGAAGAGCACACGTCTGAAC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TATTCTGGTGTCCTAGGCGTAGAGGAAAGATCGGAAGAGCACACGTCTGA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCGGAACGGCTCCTAGATCGGAAGAGCACACGTCTGAACTCCGTCACAC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TCTGTTCTATAGGATCGTACCGTTGCATCCTTAGATCGGAAGAGCACACG	1	0.3215434083601286	No Hit
CACGCCTCTCGGCAACGGATATCTCGAGATCGGAAGAGCACACGTCTGAA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTCGCACGGATTCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GATGTGGAAACCAAAAAAACCGGTAGATCGGAAGAGGACACCTCTGAATC	1	0.3215434083601286	No Hit
TCCTAACGAACGAACGATTTGAACAGATCGGAAGGGCACACGTCTGAACT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AATACGTGTGCTTGGGAGTCCTAGATCGGAAGAGCACACGTCTGAACTCC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCAACTATAGAACTATGACACAATAGATCGGAAGAGCACACGTCTGAA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGTAGCTCAGTGGTAGAGCGGTCGGCTAGATGGGAAGAGCACACGTCTG	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCAGGGTAAGAAGGGGTAAGATCGGAAGAGCACACGGCTGAACTCCAGTC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CAAACGAACGAACGATATGAACAGATCGGAAGAGCACACGTCAGAACTCC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
AACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTAAC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
ATCCGACTGTTTAATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGGTACGGACAAGGGGAATCCGACTGTTAGATCGGAAGAGCACACGTCTG	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAATTACGGTAGAGCGTGTAGATCGGAAGGGCACACGTCTGAACTCCAG	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AGAAACAATGACGGTATCTGAGAGATCGGAAGAGCACACGTCTGAACTCC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCTGCGGCAAAATAGCTCGATGCCAGAATAAGATCGGAAGAGCACACGT	1	0.3215434083601286	No Hit
TCCTCAGTAGCTCAGTGGTAGAGCGGTCGGCTAAGATCGGAAGAGCACAC	1	0.3215434083601286	No Hit
CGGACACAGGTGGTAGATCGGAAGAGCACACGTCTGAACTCCGTCACACG	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GCGGCACGTGGGCCAGATCGGAAGAGCCACGTCTGAACTCCAGTCACACG	1	0.3215434083601286	TruSeq Adapter, Index 5 (95% over 23bp)
TCCGTTGTAGTCTAGGTGGTTAGGATACTCAGATCGGAAGAGCACACGTC	1	0.3215434083601286	No Hit
ATAAGGGCGAGGTCTCTGGTTCAAGTCCAGGAAGATTGGAAGAGCACACG	1	0.3215434083601286	No Hit
TAATTCATGATCTGGCAGCTCGGAAGAGCACACCTCTGAACTCCAGACAC	1	0.3215434083601286	No Hit
GGGCGCGTTAGTGTCTGGTAATTGGAATAGATCGGAAGAGCACACGTCTG	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTAATTACCGACCGTTTTGATTCTTTAGAACAACTAGAGATCGGGAGAG	1	0.3215434083601286	No Hit
ACGACCTTCGGGCTGAAGACGTAGATCGGAAGAGTACACGTCTGAACTCC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GTGGTTGGTGTCATCTTGGGCAGATCGGAGGAGCACACGTCTGAACTCCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
CATTCACAGCAGAGGACAGCTGGAAGATCGGAAGAGCACACGTCTGAACT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGACTATGGCCGTTTAGGCCACGGAAGATCGGAAGAGCACACGTCTGA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCATACGGAATTCGAATGAAACGGAGATCGGAAGAGCACACGTCTGAACT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATAAAAGGCTGACGCGGGCTCTAGATCGGAAGAGCATACGTCTGAACTC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CACACCCAGGACGCTGAACCTGTACGAGATCGGAAGGGCACACGTCTGAA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CCGGGCCTTGTACACAAGAAAAAAAAAAAAAACGGAAGAGCACACGTATA	1	0.3215434083601286	No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACTACAAGGGAAGGGCAGACCTCT	1	0.3215434083601286	No Hit
TCCGTTGTAGTCTAGGTGGTCAGGATACTCAGATCGGAAGAGCACACGTC	1	0.3215434083601286	No Hit
TTTGGTCGGAAGGCGGCGGGGGAGATCGGAAGAGCACACGTCTGAACTCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGTATCCGATCTAGTTACACCTTTAGATCGGAAGAGCACACGTCTGAACT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACACGACTCTCGGCAACGGATATCTCAGATCGGAAGAGCACACGTCTGAA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGGTATTAGTACTATGGCATTCAACCTAAATGGTCAGATCGGAAGAGTA	1	0.3215434083601286	No Hit
TGAGGCATCCTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTC	1	0.3215434083601286	No Hit
GTCCAGACATAGCAAGGATTGACAGACTAGATCGGAAGAGCACAGTCTGA	1	0.3215434083601286	No Hit
CACGACTCTCGGCAACGGATAGATGGGAAGAGCACACGTCTGAACTCCAG	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ATAAATATAGGATCTCTGCAGCAGAGATCGGAAGAGCACACGTCTGAACT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGAGAGAATCGTGGTTAAGGAACTCGGCAGATCGGAAGAGCACACGTC	1	0.3215434083601286	No Hit
TTAGAGCATTGAGAGGACCTTTCCCTAGTAAAATCGGAAGAGCACACGTC	1	0.3215434083601286	No Hit
ACAGGATATTGGGTATTTCTAAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGTCGATGCCCGAGCGGTTAATGGGGACGGACTGTAGATCGGAAGAGCA	1	0.3215434083601286	No Hit
CGCTTGTTCGCCGGCGGTTGTGAGATCGGAAGAGCACACGTCTGAACTCC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTAACGAACGAACGATTCGAACAGATCGGAAGAGCAGACGTCTGAACTC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GCACCAGTGGTCTAGTGGTAGAATAGATCGGAAGAGCACACGTCTGAACT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCGTCACACGA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
AGACCTAGCTGCTCTAGGTGGGAAGAGCACACGTCTGAACTCCAGTCACA	1	0.3215434083601286	TruSeq Adapter, Index 1 (100% over 30bp)
CCACACTGGGACTGAGACAAGATCGGAAGAGCACACGTCTGAACTCCAGT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGAGATGTGGACACCTACGATAAGATCGGAAGAGCACGTCTGAACTCC	1	0.3215434083601286	No Hit
ACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCAATGCGCGTGCTCGTCACTGGCGCCAGATCGGAAGAGCACACGTCTG	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTTA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TCTGTAGAAAGACTGATTCATTCAGATCGAAGAGCACACGTCTGAACTCC	1	0.3215434083601286	Illumina Multiplexing Index Sequencing Primer (95% over 23bp)
TGCGATAACTGGTGTGAATTGCAGAATCCCGCGAACCATCGAGTAGATCG	1	0.3215434083601286	No Hit
TAATCGACACGACTCTCGGCAACGGATAAGATGGGAAGAGCACACGTCTG	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AAATTGTTCGGGAGACATGGTCAAAGCCCGCAAGATCGGAAGAGCACACG	1	0.3215434083601286	No Hit
TGGCTCGACTCGGCTCGAGATCGGGAGAGCACACGTCTGAACTCCAGTCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TGAAAAGGACTTTGAAAAGAGAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGATTGTAGTTCAATTGGTCAGAGCAAGATCGAAAGAGCACACGTCTGA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAATATCGGAAGAGAACACCTCTGA	1	0.3215434083601286	No Hit
TAGACCTGCTCTGGGCCACCAGGCAGATCGGAAGAGCACACGTCTGAACT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATGAACTAATTCGAACTGTGAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACTTTTCCGCGACCGTGAGCTGGGAGAGATCGGAAGAGCACACGTCTGAG	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GTAACGCGTAAGAACCTACAGATCGGAAGAGCACACGTCTGAACTCCATC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TGAATTGCAGAATCCCGCGAGATCGGAAGAGCACACGTCTGAACTCAGTC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACCTCACAACACG	1	0.3215434083601286	No Hit
TATCTAGTGAGCCTGGCTCAGACTAGATCGGAAGAGCACACGTCTGAACT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGGTACGTGCTACTCGGATAAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCAGTCACTCTG	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AAGGCTGAAACTTAAAGGAGATCGGAAGAGCACACGTCTGACTCCAGTCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ATAAAAGGCTGACGCGGGCTCTAGATCGGAAGAGCACACGTCTGAACTCC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGTCTGTAGTCCAACGGTTAGGATAAGATCGGAAGAGCAAACGACAGCC	1	0.3215434083601286	No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCAGATCGGAAGAACACACGTCT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATAGTTTGTTTGATGGTACGTGCTAGATCGGAAGAGCACACGTCTGAACC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
AAGACGGTGGATGAGCTGAAAGGCGATCTAGATCGGAAGAGCACAAGTCT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TACAGGTTGTCTGACAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGGATGATTCAGCTGCTAGCACAACTGGAATAGATCGGAAAGAGCACAC	1	0.3215434083601286	No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCAGATCGGAAGAGCACACGTCT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAATTCATGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTTACACT	1	0.3215434083601286	TruSeq Adapter, Index 8 (97% over 36bp)
CAAATCTAACGAAGTGCTCCTGGACGACAGATTGGAAGAGCACACGTCTG	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TTGACGGATCGCACGGCCTTCGTGCCGGTAGATCGGAAGAGCACACGTCT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATTCTGGTGTCCTAGGCGTAGAGGAAGATCGGAAGAGCACACGTCTGAA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCTCACCGCACCAGATCGGAAGAGCACACGTCTGAACTCCAGTAACACG	1	0.3215434083601286	TruSeq Adapter, Index 5 (97% over 35bp)
TTTTTTTTCTTTTCCCCGGCCATGGTTCCTAGATCGGAAGAGCACACGTC	1	0.3215434083601286	No Hit
TTGGCACAAACGGACGTCGTGGTAAATCGGAAGAGCACACGTCTGAACTC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ATCCAGCCCCGCGTCGCACGGATTCGAGATCGGAAGAGCACACGTCTGAA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCTCGTGTTGCATTCCAGATCGGAAGAGCACACGTCTGAACTCCGGTCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ACGACGGCCTGGTAAACGTGTCGAAGATCGGAAGAGCACGCGTCTGAACT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GTGGCTGTAGTTTAGTGGTGAGAGTTCCAAATCGGAAGAGCACACGTCTG	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCCGAACTCC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CATCGACTCTGCTATGAGGATAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAG	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCAGATCGGAAGGGCACAC	1	0.3215434083601286	No Hit
ATCCCACTTCTGACACCAAGATCGGAAGGGCACACGTCTGAACTCCAGTC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CTAACGAACGAACGATTTGAACAGATTGGAAGAGCACACGTCTGAACTCC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTGTCAAAGATCGGAAGA	1	0.3215434083601286	No Hit
GCGTTTGTAGTCCAACGGTTAGGATAATTGCCTTCTAGATCGGAAGAGCA	1	0.3215434083601286	No Hit
GGGGATGTAGCTCAGATGGTAGAGCTCGCTTGAGATCGGAAGAGCACACG	1	0.3215434083601286	No Hit
ACAACCGACTCAGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCGACTGTTTAATTAAAACAAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGGCATG	1	0.3215434083601286	TruSeq Adapter, Index 20 (97% over 38bp)
TCAAAAGAGGAAAGGCTTGCGGTGGATACCTAGGCACCCAGAGACGAGAT	1	0.3215434083601286	No Hit
CGAACGAACGATTTGAAAAGATCGAAAGAGCAAGCGACAAAGCAACAGAC	1	0.3215434083601286	No Hit
ACACGACTCTCGGCAACGGAGATCGGAAGAGCACACGTCTGAACTCCAGT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCTCAGTAGCTCAGTGGTAGAGCGGTCGGCTGAGATCGGAAGGGCACAC	1	0.3215434083601286	No Hit
CACACTATAGGAGCTGGAGATCGGAAGAGCACACGTCTGAACTCCGTCAC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CCTTATCGGATTGGACGTGCCTCGAAGAAGATCGGAAGAGCACACGTCTA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTCGATATGTCCGAGTGGTTAAGGAGATCGGAAGAGCACACGTCTGAACT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TACAACGAGCGACGACGGTGAGATCGGAAGGAGCACACGTCTGAACTCCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA	1	0.3215434083601286	TruSeq Adapter, Index 2 (97% over 36bp)
GACCGAATTCCGCCCACGAGATCGGAAGAGCACACGTCTAACTCCAGTCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GCCCACTTAGCTCAGAGGTTAGAGCATCGCATTTGAGATCGGAAGAGCAC	1	0.3215434083601286	No Hit
TGCATGTGTGTATGACTATTCGAGATAGGAAGAGCACACGTCTGAACTCC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGAGAGATGGCTGAGTGGACTAAAGCGGCGGATTGGGATCGGAAGGGCAC	1	0.3215434083601286	No Hit
AAAATAATAAAAAAAAAAAAAAAAAAAAAAGGAAAAGAACAAAACTGAAA	1	0.3215434083601286	No Hit
GAACCCATCGAGAAATTGGTAGACACCCTACTCTTAGAACGGTAAAGCAC	1	0.3215434083601286	No Hit
CACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAATCCAG	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GACACGACTCTCGGCAACGGATATCTCGGATATCGGAGCAGAACAGCACA	1	0.3215434083601286	No Hit
CTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTAAACTCC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TCGGGCAAATTATCAGATTGGAAGATCGGAAGAGCACACGTCTGAACTCC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCACTTGTGTGTCTAGACCAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGCAGAACTGGCGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTTA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GCCCCCATCGTCTAGTGTTCAGGACATCTCTCTTTAGATCGGAAGAGCAC	1	0.3215434083601286	No Hit
AACGATGATGAAAAGCTCCGTGACGCATGAGATGGAAGAGCACACGTCTG	1	0.3215434083601286	No Hit
CAAAGGGGCTTCATGTGGTGGAAGATCGGAAGAGCACACGTCTGAACTCC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCTGCGGCAAAATAGCTCGATGCCAGATCGGAAGAGCACACGTCTGAACT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAACGAACGATTTGAACAGATCGGAAGAGCACACGTCAGAACTCCAGTCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ATAGCAAGGATTGACAGACTGAGAGCTCTTTCTTGAGATCGGAAGAGCAC	1	0.3215434083601286	No Hit
TGAGGCATCCTAACGAACGAACGATTTGAACAGATGGAAGAGCACACGTC	1	0.3215434083601286	No Hit
AATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAGAACACCAA	1	0.3215434083601286	No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTAGATCGGAAGAGCACA	1	0.3215434083601286	No Hit
CTCGGATAACCGTAGTAATTCTAGAGCTAATACGTAGATCGGAAGAGCAC	1	0.3215434083601286	No Hit
GAACGAACGATTTGAACAGATCGGAGGAGCACACGTCTGAACTCCAGTCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ATCGATGATGAAAACGCTCAGTGACAAGATCGGAAGAGCACACGTCTGAA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACGACTCTCGGCAACGGATAAGATCGGAAGAGCAACGTCTGAACTCCAG	1	0.3215434083601286	No Hit
GGAGAGATGGCTGAGTGGACTAAAGCGGCGGATTAGATCGGAAGAGCACA	1	0.3215434083601286	No Hit
ATGGTACGTGCTACTCGGATAAGATCGGAAGGGCACACGTCTGAACTCCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GTGTTCGTTCCACGCTCACCGCACCAGATCGGAAGAACACACGTCTGAAC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
CCCCGCGTCGCACGGATTCGTCAGATCGGAAGAGGCACACGTCTGAACTC	1	0.3215434083601286	No Hit
AAGGAAGCTATAAGTAATGCAACTATGAAGATCGGAAGAGCACCACGTCT	1	0.3215434083601286	No Hit
GGGGATGTAGCTCAGATCGGAAGGAGCACACGTCTGAACTCCAGTCACAC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GGGATTGTAGTTCAATTAGATCGGAAGGGCACACGTCTGAACTCCAGTCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TAATTCATGATCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTCATGGAGAGTTCGATCCTGGCTAGATCGGAAGAGCACACGTCTGAAC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGGGGGCTCGAAGACGATCAGATACCGTCCTAAGATCGGAAGAGCACAC	1	0.3215434083601286	No Hit
GGGGCTGTAGCTCAGATGGGAGAGCGCTGCAGTAGATCGGAAGAGCACAC	1	0.3215434083601286	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCTAGATCGGAAGGGGA	1	0.3215434083601286	No Hit
ATGGATGGCGATCAAATGCAAGAAGATCGGAAGAGCAGACGTCTGAACTC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TTGACTTAATCGCACTCATCAGGTAGATCGGAAGAGCACACGTCTGAACT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG	1	0.3215434083601286	TruSeq Adapter, Index 7 (97% over 36bp)
CAGCCCCGCGTCGCACGGATTCGTAGATCGGAAGAGCACACGTCTGAACT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGTTCGGATCGCGGCGACTGGGGCGGTTCAGATCGGAAGAGCACCGTCT	1	0.3215434083601286	No Hit
CGATAAGTTAGACCTGCTGGGAGGAAGATCGGAAGAGCACCACGTCTGAA	1	0.3215434083601286	No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTGAGATCGGAAGAGCAC	1	0.3215434083601286	No Hit
AGGCCTACATAGAACAGGAAATGTAGATCGGAAGAGCACACGTCTGAACT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTGCGCTGCAACGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AATGAGTCTGCTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTTACA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TAACTGAGGCTGAGAGACTGTGGCAAGATCGGAAGAGCAACGTCTGAACT	1	0.3215434083601286	No Hit
GGGCGTTTGGTCTAGTGGTATGATTCTCGAGATCGGAAGAGCACACGTCT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGACGTGCTCGCCGTAGATCAGAAGAGCACACGTCTGAACTCCAGT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GATGCAAGCGTTATCCGGAATGATTGGGCGAGATCGGAAGAGCACACGTC	1	0.3215434083601286	No Hit
CATATCGGGTAGGTTGTGGTATTTCATTGCTAGATCGGAAGAGCACACGT	1	0.3215434083601286	No Hit
ATGTATGCAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGA	1	0.3215434083601286	No Hit
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCGCACG	1	0.3215434083601286	TruSeq Adapter, Index 5 (97% over 35bp)
GAAGATCGCCGTGGACATGGTTAACGAGGGAGATCGGAAGAGCACACGTC	1	0.3215434083601286	No Hit
CACCCCAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTAAACTC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ATTTAGTTGGATAAACACGAACGGAGATCGGAAGAGCACACGTCTGAACT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGACTGAACGCCTCTAAGTCAGAATGGATAGATCGGAAGAGCACACGTCT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGCAAGGTCGTGGAGGTTCGAGTCCTCTTAGATCGAAGAGCACACGTCT	1	0.3215434083601286	No Hit
GGGATTGTAGTTCAATCGGAAGAGCACACGTCTGAACCCAGTCACACGAC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
GGGTTCGTTCCTTTAGAGCGGCGATCGTCAGCTGAACCGTAGTGAAGACC	1	0.3215434083601286	No Hit
TAATAGGTAGTGTATTTGTATAAGATCGGAAGAGCACACGTCTGAACTCC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CACGCCTGTCGGGACCCGAAAGATGAGATCGGAAGAGCACACGTCTGAAC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTAACGATCTGGGCACTGTCTAGATCGGAAGAGCACACGTCTGAGCTCC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
AGGGCTATAGCTCAGATCGGAAAGAGCACACGTCTGAACTCCAGTCACAC	1	0.3215434083601286	TruSeq Adapter, Index 5 (96% over 31bp)
TCTCATGGAGAGTTCGATCCTGGCTCAGGATAGATCGGAAGAGCGCACGT	1	0.3215434083601286	No Hit
CGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGCA	1	0.3215434083601286	TruSeq Adapter, Index 7 (97% over 36bp)
AACGATTTGAACAGAACGGAAGAGCACACGTCTGAACTCCAGTCACACGT	1	0.3215434083601286	TruSeq Adapter, Index 5 (97% over 35bp)
TATGGAGGTAATGCACGAACGTAGATCGGAAGAGCACACGTCTGAACTCC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTTAC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TCCTCAGTAGCTCAGTGGTAGAGCGGTCGGCTGTAAGATCGGAAGAGCAC	1	0.3215434083601286	No Hit
AAAATCAAACAAGACGGTAAAAACAGATCGGAAGAGCACACGTCTGAACT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTCATTGTTCAACTCTTTGAAGATCGGAAGAGCACACGTCTGAACTCCG	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
AAACCTTGAGACTGTGTTTAGATCGGAAGAGCACACGTCTGAACTCCATC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTGTAGATCGGAAGAGCA	1	0.3215434083601286	No Hit
TTCTGACCGGCATTGAGAGAGCCACAGATCGGAAGAGCACACGTCTGAAC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCATTTATTAGATAAAAGTCTGACGCGGGCTCTAGATCGGAAGAGCACA	1	0.3215434083601286	No Hit
TCCGTTGTAGTCTAGGTGGTCAGGATAATAAGAGATGAAGAGACCACTGC	1	0.3215434083601286	No Hit
CCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGACCCCAGGTCAGTCGGGACTAGCTGGGAAGGGCCCACGTCGGAACTG	1	0.3215434083601286	No Hit
GATGAGACTGACCGGGGTTCGTTTCCCCGGATGCGCACCAAGATCGGAAG	1	0.3215434083601286	No Hit
TTCGGACCAGGCTTCATTCCCAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGGGTAAAGCCAATGATTAAGATCGGAAGAGCACACGTCTGACTCCAGT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GTGGTCGTGCCGGAGTGGTTATCGGGCAGATCGGAAGAGCACACGTCTGA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGAAGTGCTCCTGGACGAGATCGGAAGAGCACACGTCTGAACTCCATCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
CCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGATCGGAGGAGCAC	1	0.3215434083601286	No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCTAGATCGGAAGAGCACAC	1	0.3215434083601286	No Hit
TCAGTTGGTAGAGCAGAGGACTAGATCGGAAGAGCACACGTCTGAACTCC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAACCCTTGGCTGTCTGAGCTCCAGATCGGAAGGGCACACGTCTGAACTC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TATGCAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGA	1	0.3215434083601286	No Hit
TCTCATGGAGAGTTCGATCCTGGCTCAGGAAGATTGGAAGAGCACACGTC	1	0.3215434083601286	No Hit
CCTTTAGGAATATCTGGTACTTTCAACTAGATCGGAAGAGCACACGTCTG	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAACGGGCGGCGTGCTGAAAGATCGGAAGAGCGCACGTCTGAACTCCAG	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TCCTCAGTAGCTCAGTGGTAGAGCAGTCGGCTGAGATCGGAAGAGCACAG	1	0.3215434083601286	No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTGTAGATCGGAAGAGCA	1	0.3215434083601286	No Hit
TTTGTAGCAAGGGTGACAAGGTAAGATAGGAAGAGCACACGTCTGAACTC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ATGATTAATAGGGACAGTCGGGGGCAGGATCGGAAGAGCACACGTCTGAA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TCTCATGGAGAGTTCGATCAGATCGGAAGGGCACACGTCTGAACTCCAGT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TCAAAAGAGGAAAGGCTTGCGGTGGATACCAGATCGGAAGAGCACACGTC	1	0.3215434083601286	No Hit
GAAGTTATGGTAAAAGGTGCTGGTAGCGGAGATGGGAAGAGCACACGTCT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTGATGGTATGCCTTTAGGAATATCTGGTACTTTCATCTAGATCGGAAGA	1	0.3215434083601286	No Hit
TAGCTCAGTTCGGTAGAGCAACTCGTTTAGATCGGAAGAGCACGCGTCTG	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCGAGTGAATCACGGCTAAGGAACTCGGCAGATCGGAAGAGCACACGTCT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAGATCGGAAGA	1	0.3215434083601286	No Hit
CCGACCCGCGCCGCGAGATCGGAAGAGCACACGTCTGAACTCCATCACAC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
CGACACGACTCTCGGCAACGGATATCTCGGCTCTCAGATCGGAAGAGCAC	1	0.3215434083601286	No Hit
ATTTGACCCTTAGCCCCGGTGTAGATCGGAAGAGCACACGTCTGAACTCC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGTGTTTGGTCTAGTGGTATGATTCTCGCTTCGAGATCGGAAGAGCACA	1	0.3215434083601286	No Hit
CTTGTTCGTTTCTGTCGGATTGGTAGATCGGAAGAGCACACGTCTGAACT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAATTCATGATCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGGACAAGGGGAAAGATCGGAAGAGCACACGTCTGAACTCGAGTCACACG	1	0.3215434083601286	TruSeq Adapter, Index 5 (97% over 35bp)
ACTTGACGGATCGCACGGCCTTCGTGCCGGCGAGATCGGAAGAGCACACG	1	0.3215434083601286	No Hit
CGGACAAGGGGAATCCGACTGTTTAATAGATCGGAAGAGCAAACGTCTGA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ATCTCTGACCGCGTGCCTGAGATCGGAAGAGCACACGTCTGAACACCAGT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
CACGACTCTCGGCAACGAGATCGGAAGAGCACACGTCTGAACTCCAGTTA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TGACGGGTGACGGAGAATTAGGGTTCGATTCCGAGATCGGAAGAGCACGC	1	0.3215434083601286	No Hit
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAATCCAGTCACACGA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GGGTCGATGCCCGAGCGGTTAATGGGGACGGACTGAGATCGGAAGAGCAC	1	0.3215434083601286	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTAAGGTAGGAGCAGACCTCTTC	1	0.3215434083601286	No Hit
AACGTCAGCAATCTCTCTCGTCTGAGATCGGAAGAGCACACGTCTGAACT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCAGATCGGAGGAGCAC	1	0.3215434083601286	No Hit
CGGTCTCCGATCGTTTGGTTCCCAGTAGACTCGAGATCGGAAGAGCACAC	1	0.3215434083601286	No Hit
GATACCTGGTGTGAATTGCAGAATCCCGTAGATCGGAAGAGCACACGTCT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGAACTGTGAAACTGCGAATGGCTCATTAAATCAGATCGGAAGAGCACAC	1	0.3215434083601286	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCAGATCGGAAGAGCACACGTC	1	0.3215434083601286	No Hit
ATTTTTCAGTGAGGACAAGCAAGAAATCGGAAGAGCACACGTCTGAAGTC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
ACCCTTCTGCCGGCGATGCGCTCCTGGCCTTAGATCGGAAGATCACACGT	1	0.3215434083601286	No Hit
GAACGAACGATTTGAACAGATCGGAAGAGCACACTCTGAACTCCAGTCAC	1	0.3215434083601286	No Hit
AGCTCAGCTGGCTAGAGCGCCTGAGATCAGAAGAGCACACGTCTGAACTC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CCCAAGATGAGTGCTCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GGGATTGTAGTTCAATTGGTCAGAGCAGATCGGAAGAGCACACGTCTGAA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTAAGAGGACCGGTTAGCAGATCGGGAGAGCACACGTCTGAACTCCAGT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GGGATTGTAGTTCAATTAGATCGGAAGAGCAAACGTCTGAACTCCAGTCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCATCACACGA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCAGATCGGAAGAGCACAC	1	0.3215434083601286	No Hit
TCTTTTGATTAGAGTAGAGAGATAGGAAGAGCACACGTCTGAACTCCAGT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GGGTCGATGCCCGAGCGGTTAATGGGGACGAGATCGGAAGAGCACACGTC	1	0.3215434083601286	No Hit
CCAAGAGGTTACTCTAGACGGAGCAGATCGGAAGAGCACACGTCTGAACT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGCGTGTCGCTCCAGAACCTCAAAGATCGGAAGGGCACACGTCTGAAC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
CCGACTTTGTGAAATGAGATCGGAGGAGCACACGTCTGAACTCCAGTCAC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TCCTCAGTAGCTCAGTGGTAGAGCGGTCGGCTGAGATCGGAAGAACACAC	1	0.3215434083601286	No Hit
GTGTGAATTGCAGAATCCCGCGAACCAGATCGGAAGAGCACACGCTGAAC	1	0.3215434083601286	No Hit
CCAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGACCCCAGGTCAGTCGGGACTACCAGATCGGAAGAGCACACGTCTGAA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACACCGCCTACGACGTGGACCAGATCGGAAGAGCACACGTCTGAACTC	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCA	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CGCGACCCCAGGTCAGTCGGGACTAGATCGGAAGAGCACACGTCTGAACT	1	0.3215434083601286	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.3215434083601286	0.0	0.0	0.0	0.0
10	0.3215434083601286	0.0	0.0	0.0	0.0
11	0.6430868167202572	0.0	0.0	0.0	0.0
12	0.6430868167202572	0.0	0.0	0.0	0.0
13	0.6430868167202572	0.0	0.0	0.0	0.0
14	3.8585209003215435	0.0	0.0	0.0	0.0
15	4.501607717041801	0.0	0.0	0.0	0.0
16	5.787781350482315	0.0	0.0	0.0	0.0
17	7.717041800643087	0.0	0.0	0.0	0.0
18	10.610932475884244	0.0	0.0	0.0	0.0
19	12.218649517684888	0.0	0.0	0.0	0.0
20	15.434083601286174	0.0	0.0	0.0	0.0
21	16.39871382636656	0.0	0.0	0.0	0.0
22	22.186495176848876	0.0	0.0	0.0	0.0
23	31.832797427652732	0.0	0.0	0.0	0.0
24	33.762057877813504	0.0	0.0	0.0	0.0
25	41.47909967845659	0.0	0.0	0.0	0.0
26	43.72990353697749	0.0	0.0	0.0	0.0
27	47.90996784565916	0.0	0.0	0.0	0.0
28	49.839228295819936	0.0	0.0	0.0	0.0
29	52.41157556270097	0.0	0.0	0.0	0.0
30	54.98392282958199	0.0	0.0	0.0	0.0
31	59.80707395498392	0.0	0.0	0.0	0.0
32	61.09324758842444	0.0	0.0	0.0	0.0
33	63.344051446945336	0.0	0.0	0.0	0.0
34	66.2379421221865	0.0	0.0	0.0	0.0
35	67.52411575562701	0.0	0.0	0.0	0.0
36	72.34726688102894	0.0	0.0	0.0	0.0
37	73.95498392282958	0.0	0.0	0.0	0.0
38	73.95498392282958	0.0	0.0	0.0	0.0
39	74.59807073954984	0.0	0.0	0.0	0.0
40	75.88424437299035	0.0	0.0	0.0	0.0
41	76.20578778135048	0.0	0.0	0.0	0.0
42	76.20578778135048	0.0	0.0	0.0	0.0
43	76.52733118971061	0.0	0.0	0.0	0.0
44	76.52733118971061	0.0	0.0	0.0	0.0
45	76.84887459807074	0.0	0.0	0.0	0.0
46	76.84887459807074	0.0	0.0	0.0	0.0
47	76.84887459807074	0.0	0.0	0.0	0.0
48	76.84887459807074	0.0	0.0	0.0	0.0
49	76.84887459807074	0.0	0.0	0.0	0.0
50	77.17041800643086	0.0	0.0	0.0	0.0
51	77.17041800643086	0.0	0.0	0.0	0.0
52	77.17041800643086	0.0	0.0	0.0	0.0
53	77.17041800643086	0.0	0.0	0.0	0.0
54	77.17041800643086	0.0	0.0	0.0	0.0
55	77.17041800643086	0.0	0.0	0.0	0.0
56	77.17041800643086	0.0	0.0	0.0	0.0
57	77.17041800643086	0.0	0.0	0.0	0.0
58	77.17041800643086	0.0	0.0	0.0	0.0
59	77.17041800643086	0.0	0.0	0.0	0.0
60	77.17041800643086	0.0	0.0	0.0	0.0
61	77.17041800643086	0.0	0.0	0.0	0.0
62	77.17041800643086	0.0	0.0	0.0	0.0
63	77.17041800643086	0.0	0.0	0.0	0.0
64	77.17041800643086	0.0	0.0	0.0	0.0
65	77.17041800643086	0.0	0.0	0.0	0.0
66	77.17041800643086	0.0	0.0	0.0	0.0
67	77.17041800643086	0.0	0.0	0.0	0.0
68	77.17041800643086	0.0	0.0	0.0	0.0
69	77.17041800643086	0.0	0.0	0.0	0.0
70	77.17041800643086	0.0	0.0	0.0	0.0
71	77.17041800643086	0.0	0.0	0.0	0.0
72	77.17041800643086	0.0	0.0	0.0	0.0
73	77.17041800643086	0.0	0.0	0.0	0.0
74	77.17041800643086	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Rejected 15 READS because READLEN < 1
Read 15 spots for SRR11699580.sra
Written 15 spots for SRR11699580.sra
Rejected 15 READS because READLEN < 1
Read 15 spots for SRR11699580.sra
Written 15 spots for SRR11699580.sra
Rejected 15 READS because READLEN < 1
Read 15 spots for SRR11699580.sra
Written 15 spots for SRR11699580.sra
Rejected 15 READS because READLEN < 1
Read 15 spots for SRR11699580.sra
Written 15 spots for SRR11699580.sra
Rejected 15 READS because READLEN < 1
Read 15 spots for SRR11699580.sra
Written 15 spots for SRR11699580.sra
Rejected 15 READS because READLEN < 1
Read 15 spots for SRR11699580.sra
Written 15 spots for SRR11699580.sra
Rejected 15 READS because READLEN < 1
Read 15 spots for SRR11699580.sra
Written 15 spots for SRR11699580.sra
Rejected 15 READS because READLEN < 1
Read 15 spots for SRR11699580.sra
Written 15 spots for SRR11699580.sra
Rejected 15 READS because READLEN < 1
Read 15 spots for SRR11699580.sra
Written 15 spots for SRR11699580.sra
Rejected 15 READS because READLEN < 1
Read 15 spots for SRR11699580.sra
Written 15 spots for SRR11699580.sra
Rejected 15 READS because READLEN < 1
Read 15 spots for SRR11699580.sra
Written 15 spots for SRR11699580.sra
Rejected 15 READS because READLEN < 1
Read 15 spots for SRR11699580.sra
Written 15 spots for SRR11699580.sra
Rejected 15 READS because READLEN < 1
Read 15 spots for SRR11699580.sra
Written 15 spots for SRR11699580.sra
Rejected 15 READS because READLEN < 1
Read 15 spots for SRR11699580.sra
Written 15 spots for SRR11699580.sra
Rejected 15 READS because READLEN < 1
Read 15 spots for SRR11699580.sra
Written 15 spots for SRR11699580.sra
Rejected 15 READS because READLEN < 1
Read 15 spots for SRR11699580.sra
Written 15 spots for SRR11699580.sra
Rejected 15 READS because READLEN < 1
Rejected 15 READS because READLEN < 1
Read 15 spots for SRR11699580.sra
Read 15 spots for SRR11699580.sra
Written 15 spots for SRR11699580.sra
Written 15 spots for SRR11699580.sra
Rejected 15 READS because READLEN < 1
Read 15 spots for SRR11699580.sra
Written 15 spots for SRR11699580.sra
Rejected 26 READS because READLEN < 1
Read 26 spots for SRR11699580.sra
Written 26 spots for SRR11699580.sra
SRR ids: ['SRR11699580.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_qavieapi
SRR11699580.sra spots: 311
blocks: [[1, 15], [16, 30], [31, 45], [46, 60], [61, 75], [76, 90], [91, 105], [106, 120], [121, 135], [136, 150], [151, 165], [166, 180], [181, 195], [196, 210], [211, 225], [226, 240], [241, 255], [256, 270], [271, 285], [286, 311]]
SRR11699580 file size 63
SRR11699580 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR11699580 SRR11699580_1.fastq
Input file:	SRR11699580_1.fastq
trimmed:	SRR11699580-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	20
Sat Jun 21 05:14:18 2025 >> started

Sat Jun 21 05:14:18 2025 >> done (0.001s)
311 reads processed; of these:
 42 (13.50%) short reads filtered out after trimming by size control
  0 ( 0.00%) empty reads filtered out after trimming by size control
269 (86.50%) reads available; of these:
252 (93.68%) trimmed reads available after processing
 17 ( 6.32%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  6	  2.23%
 19	 14	  5.20%
 20	  6	  2.23%
 21	 20	  7.43%
 22	 31	 11.52%
 23	 12	  4.46%
 24	 24	  8.92%
 25	 11	  4.09%
 26	 14	  5.20%
 27	  7	  2.60%
 28	 12	  4.46%
 29	 11	  4.09%
 30	 18	  6.69%
 31	  6	  2.23%
 32	  9	  3.35%
 33	 12	  4.46%
 34	  4	  1.49%
 35	 18	  6.69%
 36	  5	  1.86%
 37	  0	  0.00%
 38	  3	  1.12%
 39	  4	  1.49%
 40	  1	  0.37%
 41	  0	  0.00%
 42	  1	  0.37%
 43	  0	  0.00%
 44	  1	  0.37%
 45	  0	  0.00%
 46	  1	  0.37%
 47	  0	  0.00%
 48	  0	  0.00%
 49	  1	  0.37%
 50	  0	  0.00%
 51	  0	  0.00%
 52	  0	  0.00%
 53	  0	  0.00%
 54	  0	  0.00%
 55	  0	  0.00%
 56	  0	  0.00%
 57	  0	  0.00%
 58	  0	  0.00%
 59	  0	  0.00%
 60	  0	  0.00%
 61	  0	  0.00%
 62	  0	  0.00%
 63	  0	  0.00%
 64	  0	  0.00%
 65	  0	  0.00%
 66	  0	  0.00%
 67	  0	  0.00%
 68	  0	  0.00%
 69	  0	  0.00%
 70	  0	  0.00%
 71	  0	  0.00%
 72	  0	  0.00%
 73	  0	  0.00%
 74	  0	  0.00%
 75	  0	  0.00%
 76	  0	  0.00%
 77	  0	  0.00%
 78	  0	  0.00%
 79	  0	  0.00%
 80	  0	  0.00%
 81	  0	  0.00%
 82	  0	  0.00%
 83	  0	  0.00%
 84	  0	  0.00%
 85	  0	  0.00%
 86	 17	  6.32%
269 reads passed initial QC
                                 Started job on |	Jun 21 05:14:27
                             Started mapping on |	Jun 21 05:14:28
                                    Finished on |	Jun 21 05:14:28
       Mapping speed, Million of reads per hour |	inf

                          Number of input reads |	269
                      Average input read length |	30
                                    UNIQUE READS:
                   Uniquely mapped reads number |	40
                        Uniquely mapped reads % |	14.87%
                          Average mapped length |	24.40
                       Number of splices: Total |	1
            Number of splices: Annotated (sjdb) |	0
                       Number of splices: GT/AG |	1
                       Number of splices: GC/AG |	0
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	0
                      Mismatch rate per base, % |	0.92%
                         Deletion rate per base |	0.00%
                        Deletion average length |	0.00
                        Insertion rate per base |	0.00%
                       Insertion average length |	0.00
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	26
             % of reads mapped to multiple loci |	9.67%
        Number of reads mapped to too many loci |	67
             % of reads mapped to too many loci |	24.91%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	12.27%
                     % of reads unmapped: other |	38.29%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	203	203	203
N_multimapping	26	26	26
N_noFeature	25	27	37
N_ambiguous	1	0	0
UnstrandedReadsAssigned:14 PositiveStrandReadsAssigned:13 NegativeStrandReadsAssigned:3
Dataset is classified unstranded
MeadianReadLen=26 20thPercentileLength=22 echo kmer=19
SRR11699580 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 72,539
[index] number of k-mers: 56,563,136
[index] number of equivalence classes: 414,827
[quant] running in single-end mode
[quant] will process file 1: SRR11699580-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 269 reads, 56 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 362 rounds

  72540 SRR11699580.ke.tsv
  44303 SRR11699580.se.tsv
 116843 total
==> SRR11699580.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Zm00001eb096110_T001	2067	1968	0	0
Zm00001eb321680_T001	3417	3318	0	0
Zm00001eb323640_T001	3273	3174	0	0
Zm00001eb314140_T001	893	794	0	0
Zm00001eb080260_T001	3529	3430	0	0
Zm00001eb026490_T001	2433	2334	0	0
Zm00001eb026490_T002	2229	2130	0	0
Zm00001eb026490_T003	2220	2121	0	0
Zm00001eb281360_T001	2412	2313	0	0

==> SRR11699580.se.tsv <==
Zm00001eb015280	0
Zm00001eb000610	0
Zm00001eb033210	0
Zm00001eb044610	0
Zm00001eb014360	0
Zm00001eb051320	0
Zm00001eb021860	0
Zm00001eb011690	0
Zm00001eb016840	0
Zm00001eb052060	0
SRR11699580 completed mapping pipeline successfully
