Starting /dee2/code/volunteer_pipeline.sh SRR11699265
    current disk space = 2472752189440
    free memory = 1581612184 
SRR11699265 SRAfilesize
3ed89669e4f235abafa40178d20e4caf  SRR11699265.sra
SRR11699265.sra file validated
SRR11699265 is single end
SRR11699265 is conventional basespace
SRR11699265 read1 length is 76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR11699265_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24675	32.0	32.0	32.0	32.0	32.0
2	31.276	32.0	32.0	32.0	32.0	32.0
3	31.37875	32.0	32.0	32.0	32.0	32.0
4	31.50875	32.0	32.0	32.0	32.0	32.0
5	31.487	32.0	32.0	32.0	32.0	32.0
6	34.965	36.0	36.0	36.0	36.0	36.0
7	34.936	36.0	36.0	36.0	36.0	36.0
8	35.03775	36.0	36.0	36.0	36.0	36.0
9	34.76025	36.0	36.0	36.0	32.0	36.0
10-11	35.0505	36.0	36.0	36.0	36.0	36.0
12-13	35.060249999999996	36.0	36.0	36.0	36.0	36.0
14-15	34.981625	36.0	36.0	36.0	36.0	36.0
16-17	35.091750000000005	36.0	36.0	36.0	36.0	36.0
18-19	35.0905	36.0	36.0	36.0	36.0	36.0
20-21	34.90475	36.0	36.0	36.0	34.0	36.0
22-23	34.918	36.0	36.0	36.0	36.0	36.0
24-25	35.008875	36.0	36.0	36.0	36.0	36.0
26-27	34.796	36.0	36.0	36.0	34.0	36.0
28-29	34.8745	36.0	36.0	36.0	36.0	36.0
30-31	34.86175	36.0	36.0	36.0	36.0	36.0
32-33	34.91675	36.0	36.0	36.0	36.0	36.0
34-35	34.865625	36.0	36.0	36.0	34.0	36.0
36-37	34.876875	36.0	36.0	36.0	36.0	36.0
38-39	34.88275	36.0	36.0	36.0	36.0	36.0
40-41	34.8035	36.0	36.0	36.0	36.0	36.0
42-43	34.74625	36.0	36.0	36.0	32.0	36.0
44-45	34.678625	36.0	36.0	36.0	32.0	36.0
46-47	34.67225	36.0	36.0	36.0	32.0	36.0
48-49	34.597	36.0	36.0	36.0	32.0	36.0
50-51	34.25375	36.0	36.0	36.0	32.0	36.0
52-53	33.974125	36.0	36.0	36.0	32.0	36.0
54-55	33.803375	36.0	36.0	36.0	29.5	36.0
56-57	32.99075	36.0	36.0	36.0	17.5	36.0
58-59	32.123625	36.0	32.0	36.0	14.0	36.0
60-61	31.52825	36.0	32.0	36.0	14.0	36.0
62-63	30.480874999999997	36.0	29.5	36.0	14.0	36.0
64-65	29.111874999999998	36.0	20.5	36.0	14.0	36.0
66-67	27.26775	32.0	14.0	36.0	14.0	36.0
68-69	26.41325	32.0	14.0	36.0	14.0	36.0
70-71	26.049500000000002	32.0	14.0	36.0	14.0	36.0
72-73	26.04725	29.5	14.0	36.0	14.0	36.0
74-75	25.938875000000003	32.0	14.0	36.0	14.0	36.0
76	23.49125	21.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	0.0
21	2.0
22	5.0
23	8.0
24	17.0
25	43.0
26	50.0
27	72.0
28	106.0
29	199.0
30	285.0
31	331.0
32	496.0
33	774.0
34	1079.0
35	531.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.45	30.25	22.475	22.825
2	21.375	28.575	27.325	22.725
3	22.075	33.0	18.175	26.75
4	28.9	31.7	18.55	20.849999999999998
5	20.974999999999998	27.925	23.375	27.725
6	28.325	27.975	25.074999999999996	18.625
7	29.725	35.275	17.474999999999998	17.525
8	23.575	26.375	29.875	20.175
9	24.5	33.45	17.150000000000002	24.9
10-11	30.562499999999996	30.125	22.375	16.9375
12-13	22.9375	23.75	23.2625	30.049999999999997
14-15	29.2911613951744	38.56732091511439	17.127140892611575	15.014376797099638
16-17	23.925	23.962500000000002	31.924999999999997	20.1875
18-19	36.3625	24.425	24.099999999999998	15.1125
20-21	23.275000000000002	35.6625	24.65	16.412499999999998
22-23	30.9875	38.4375	14.4125	16.162499999999998
24-25	28.3625	38.2	15.475	17.962500000000002
26-27	28.7375	31.175000000000004	18.787499999999998	21.3
28-29	32.550000000000004	29.5375	15.787499999999998	22.125
30-31	32.3125	35.9625	11.450000000000001	20.275000000000002
32-33	30.2	36.9625	11.3	21.5375
34-35	29.6625	31.85	13.7875	24.7
36-37	26.35	34.7375	11.075	27.8375
38-39	23.9	33.8125	13.750000000000002	28.537499999999998
40-41	25.2625	28.6625	17.5875	28.487499999999997
42-43	25.75	28.050000000000004	19.3875	26.8125
44-45	22.475	32.800000000000004	16.650000000000002	28.075
46-47	20.8	30.362499999999997	16.900000000000002	31.937500000000004
48-49	16.25	29.825000000000003	18.862499999999997	35.0625
50-51	17.0125	29.225	20.525	33.2375
52-53	20.7375	25.5375	23.075000000000003	30.65
54-55	18.275	28.8625	22.6875	30.175
56-57	17.275	30.8	23.974999999999998	27.950000000000003
58-59	21.175	26.5875	26.85	25.387500000000003
60-61	22.05	25.05	29.812499999999996	23.0875
62-63	18.4125	28.962500000000002	29.825000000000003	22.8
64-65	15.562500000000002	25.55	31.0375	27.85
66-67	16.9125	19.037499999999998	34.2875	29.762499999999996
68-69	18.475	20.075000000000003	35.7875	25.662499999999998
70-71	19.675	19.6375	37.175000000000004	23.5125
72-73	20.9	15.837499999999999	34.112500000000004	29.15
74-75	20.4625	15.437500000000002	34.5125	29.5875
76	12.575	13.15	44.4	29.875
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.5
28	0.5
29	0.0
30	0.0
31	0.0
32	0.5
33	1.0
34	0.5
35	0.0
36	2.0
37	4.0
38	5.5
39	10.0
40	26.0
41	57.0
42	75.0
43	109.5
44	222.5
45	437.5
46	574.0
47	714.5
48	715.0
49	519.0
50	463.0
51	410.5
52	291.0
53	187.0
54	150.0
55	129.0
56	84.5
57	39.5
58	18.0
59	13.5
60	7.5
61	4.5
62	3.0
63	2.0
64	1.0
65	2.5
66	2.5
67	1.0
68	0.5
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.5
89	1.0
90	0.5
91	0.5
92	1.0
93	0.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0125
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.849999999999994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.37947494033412	56.175
2	5.0914876690533015	6.4
3	1.511535401750199	2.85
4	1.1137629276054097	2.8000000000000003
5	0.35799522673031026	1.125
6	0.5568814638027049	2.1
7	0.47732696897374705	2.1
8	0.15910898965791567	0.8
9	0.11933174224343676	0.675
>10	1.1137629276054097	14.924999999999999
>50	0.07955449482895784	4.2
>100	0.03977724741447892	5.8500000000000005
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCC	234	5.8500000000000005	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAAC	85	2.125	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCA	83	2.075	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACT	48	1.2	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAG	44	1.0999999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	42	1.05	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTGAGATCGGAAGAGCAC	42	1.05	No Hit
GAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	40	1.0	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGT	31	0.775	TruSeq Adapter, Index 15 (100% over 37bp)
ATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTGAAT	26	0.65	TruSeq Adapter, Index 15 (97% over 39bp)
AACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	25	0.625	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGT	23	0.575	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCCTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACT	23	0.575	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTC	22	0.5499999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATG	19	0.475	TruSeq Adapter, Index 15 (100% over 36bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCTAGATCGGAAGAGCA	19	0.475	No Hit
AACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGT	17	0.42500000000000004	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGGCTATAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATG	17	0.42500000000000004	TruSeq Adapter, Index 15 (100% over 36bp)
TAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCA	15	0.375	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	14	0.35000000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAATTCATGATCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	14	0.35000000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT	13	0.325	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTAACGAACGAACGATTTGAAGATCGGAAGAGCACACGTCTGAACTCCAG	13	0.325	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTC	13	0.325	No Hit
CGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTGA	12	0.3	TruSeq Adapter, Index 15 (97% over 39bp)
GGGGATGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT	12	0.3	TruSeq Adapter, Index 1 (100% over 35bp)
GCGAGCGTAGTTCAGTGGTAAAACATCTCCTTGCCAAGGAGAGATCGGAA	11	0.27499999999999997	No Hit
CCTGCGGCAAAATAGCTCGATGCCAGAATAGATCGGAAGAGCACACGTCT	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTTGTAGTCTAGCTGGTCAGGATACTCAGATCGGAAGAGCACACGTC	11	0.27499999999999997	No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCAGATCGGAAGAGCACAC	10	0.25	No Hit
TGCAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGC	9	0.22499999999999998	No Hit
GATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTGAA	9	0.22499999999999998	TruSeq Adapter, Index 15 (97% over 39bp)
ACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT	8	0.2	TruSeq Adapter, Index 1 (100% over 35bp)
TATGCAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGA	8	0.2	No Hit
CTAACGAACGAACGATTTGAATAGATCGGAAGAGCACACGTCTGAACTCC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACAC	7	0.17500000000000002	No Hit
TATTCTGGTGTCCTAGGCGTAGAGGAACCACACCAGATCGGAAGAGCACA	7	0.17500000000000002	No Hit
AAATAGCTCGATGCCAGAATAGATCGGAAGAGCACACGTCTGAACTCCAG	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GACACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACTC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACACGACTCTCGGCAACGGATATCTCGGCAGATCGGAAGAGCACACGTC	7	0.17500000000000002	No Hit
CATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAA	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAGGCATCCTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTC	7	0.17500000000000002	No Hit
CGACACGACTCTCGGCAACGGATATCTCGGCAGATCGGAAGAGCACACGT	7	0.17500000000000002	No Hit
ATCCTAACGAACGAACGATTAGATCGGAAGAGCACACGTCTGAACTCCAG	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAATTCATGATCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGATGTAGCGCAGCTTGGTAGCGCTTTTGTTTTAGATCGGAAGAGCAC	7	0.17500000000000002	No Hit
TATTCTGGTGTCCTAGGCGTAGAGGAACCACAAGATCGGAAGAGCACACG	7	0.17500000000000002	No Hit
CGACACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACT	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCAGATCGGAAGAGCACACG	6	0.15	No Hit
CACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACACGACTCTCGGCAACGAGATCGGAAGAGCACACGTCTGAACTCCAG	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGT	6	0.15	No Hit
ACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACA	6	0.15	No Hit
ATCCTAACGAACGAACGATTTGAAGATCGGAAGAGCACACGTCTGAACTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACCCCAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCGCCATGGTGAAATTGGTAGACACGCTGCTCTTAGATCGGAAGAGCAC	6	0.15	No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTAGATCGGAAGAGCACA	6	0.15	No Hit
CACGACTCTCGGCAACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCAC	6	0.15	No Hit
ACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTG	6	0.15	TruSeq Adapter, Index 15 (97% over 37bp)
ACACATGCAAATCTAACGAAGTGCTCCTGGACGAGATCGGAAGAGCACAC	5	0.125	No Hit
GCAGTGATGTATGCAGCTGAGGCATCCTAACGAACGAACGATTTGAACAG	5	0.125	No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTAAGATCGGAAGAGCAC	5	0.125	No Hit
AAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTGGATTGAAGGGAGCTCTGAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTGAGATCGGAAGAGCAC	5	0.125	No Hit
GGGCCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATG	5	0.125	TruSeq Adapter, Index 15 (100% over 36bp)
GCACCAGTGGTCTAGTGGTAGAATAGATCGGAAGAGCACACGTCTGAACT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGACCCCAGGTCAGTCGGGACTAGATCGGAAGAGCACACGTCTGAACTC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.075	0.0	0.0	0.0	0.0
2	0.125	0.0	0.0	0.0	0.0
3	0.25	0.0	0.0	0.0	0.0
4	0.275	0.0	0.0	0.0	0.0
5	0.3	0.0	0.0	0.0	0.0
6	0.4	0.0	0.0	0.0	0.0
7	0.475	0.0	0.0	0.0	0.0
8	0.675	0.0	0.0	0.0	0.0
9	1.55	0.0	0.0	0.0	0.0
10	2.075	0.0	0.0	0.0	0.0
11	2.65	0.0	0.0	0.0	0.0
12	3.225	0.0	0.0	0.0	0.0
13	4.675	0.0	0.0	0.0	0.0
14	6.375	0.0	0.0	0.0	0.0
15	7.9	0.0	0.0	0.0	0.0
16	9.8	0.0	0.0	0.0	0.0
17	12.675	0.0	0.0	0.0	0.0
18	15.075	0.0	0.0	0.0	0.0
19	17.225	0.0	0.0	0.0	0.0
20	20.75	0.0	0.0	0.0	0.0
21	24.775	0.0	0.0	0.0	0.0
22	30.65	0.0	0.0	0.0	0.0
23	40.05	0.0	0.0	0.0	0.0
24	43.6	0.0	0.0	0.0	0.0
25	50.35	0.0	0.0	0.0	0.0
26	54.95	0.0	0.0	0.0	0.0
27	57.7	0.0	0.0	0.0	0.0
28	61.95	0.0	0.0	0.0	0.0
29	64.05	0.0	0.0	0.0	0.0
30	66.65	0.0	0.0	0.0	0.0
31	70.15	0.0	0.0	0.0	0.0
32	72.775	0.0	0.0	0.0	0.0
33	75.175	0.0	0.0	0.0	0.0
34	77.925	0.0	0.0	0.0	0.0
35	81.025	0.0	0.0	0.0	0.0
36	84.675	0.0	0.0	0.0	0.0
37	86.75	0.0	0.0	0.0	0.0
38	88.2	0.0	0.0	0.0	0.0
39	89.075	0.0	0.0	0.0	0.0
40	90.25	0.0	0.0	0.0	0.0
41	91.025	0.0	0.0	0.0	0.0
42	92.2	0.0	0.0	0.0	0.0
43	92.525	0.0	0.0	0.0	0.0
44	92.975	0.0	0.0	0.0	0.0
45	93.35	0.0	0.0	0.0	0.0
46	93.875	0.0	0.0	0.0	0.0
47	94.125	0.0	0.0	0.0	0.0
48	94.35	0.0	0.0	0.0	0.0
49	94.575	0.0	0.0	0.0	0.0
50	94.675	0.0	0.0	0.0	0.0
51	94.8	0.0	0.0	0.0	0.0
52	94.925	0.0	0.0	0.0	0.0
53	94.95	0.0	0.0	0.0	0.0
54	94.975	0.0	0.0	0.0	0.0
55	94.975	0.0	0.0	0.0	0.0
56	95.0	0.0	0.0	0.0	0.0
57	95.025	0.0	0.0	0.0	0.0
58	95.025	0.0	0.0	0.0	0.0
59	95.05	0.0	0.0	0.0	0.0
60	95.075	0.0	0.0	0.0	0.0
61	95.075	0.0	0.0	0.0	0.0
62	95.075	0.0	0.0	0.0	0.0
63	95.1	0.0	0.0	0.0	0.0
64	95.1	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTCAG	30	7.878043E-8	70.00001	17
GATATTC	15	0.0020658788	70.00001	24
TTGTAGT	35	2.6138878E-9	70.00001	5
AGGATAT	15	0.0020658788	70.00001	22
TAGCTCA	15	0.0020658788	70.00001	8
GGATATT	15	0.0020658788	70.00001	23
ATTCGGC	15	0.0020658788	70.00001	27
TGTAGTC	30	7.878043E-8	70.00001	6
TATTCGG	15	0.0020658788	70.00001	26
ATATTCG	15	0.0020658788	70.00001	25
TTCGGCT	15	0.0020658788	70.00001	28
TCTAGCT	20	7.003785E-5	70.0	11
TCAGGAT	25	2.3570374E-6	70.0	20
GCTGGTC	20	7.003785E-5	70.0	15
TAGCTGG	20	7.003785E-5	70.0	13
CCGTTGT	25	2.3570374E-6	70.0	2
TAGTCTA	25	2.3570374E-6	70.0	8
GGTCAGG	25	2.3570374E-6	70.0	18
CTGGTCA	20	7.003785E-5	70.0	16
CTAGCTG	20	7.003785E-5	70.0	12
>>END_MODULE
Rejected 166815 READS because READLEN < 1
Read 166815 spots for SRR11699265.sra
Written 166815 spots for SRR11699265.sra
Rejected 166815 READS because READLEN < 1
Read 166815 spots for SRR11699265.sra
Written 166815 spots for SRR11699265.sra
Rejected 166815 READS because READLEN < 1
Read 166815 spots for SRR11699265.sra
Written 166815 spots for SRR11699265.sra
Rejected 166815 READS because READLEN < 1
Read 166815 spots for SRR11699265.sra
Written 166815 spots for SRR11699265.sra
Rejected 166815 READS because READLEN < 1
Read 166815 spots for SRR11699265.sra
Written 166815 spots for SRR11699265.sra
Rejected 166815 READS because READLEN < 1
Read 166815 spots for SRR11699265.sra
Written 166815 spots for SRR11699265.sra
Rejected 166815 READS because READLEN < 1
Read 166815 spots for SRR11699265.sra
Written 166815 spots for SRR11699265.sra
Rejected 166815 READS because READLEN < 1
Read 166815 spots for SRR11699265.sra
Written 166815 spots for SRR11699265.sra
Rejected 166815 READS because READLEN < 1
Read 166815 spots for SRR11699265.sra
Written 166815 spots for SRR11699265.sra
Rejected 166815 READS because READLEN < 1
Read 166815 spots for SRR11699265.sra
Written 166815 spots for SRR11699265.sra
Rejected 166815 READS because READLEN < 1
Read 166815 spots for SRR11699265.sra
Written 166815 spots for SRR11699265.sra
Rejected 166815 READS because READLEN < 1
Read 166815 spots for SRR11699265.sra
Written 166815 spots for SRR11699265.sra
Rejected 166815 READS because READLEN < 1
Read 166815 spots for SRR11699265.sra
Written 166815 spots for SRR11699265.sra
Rejected 166815 READS because READLEN < 1
Read 166815 spots for SRR11699265.sra
Written 166815 spots for SRR11699265.sra
Rejected 166815 READS because READLEN < 1
Read 166815 spots for SRR11699265.sra
Written 166815 spots for SRR11699265.sra
Rejected 166815 READS because READLEN < 1
Read 166815 spots for SRR11699265.sra
Written 166815 spots for SRR11699265.sra
Rejected 166815 READS because READLEN < 1
Read 166815 spots for SRR11699265.sra
Written 166815 spots for SRR11699265.sra
Rejected 166815 READS because READLEN < 1
Read 166815 spots for SRR11699265.sra
Written 166815 spots for SRR11699265.sra
Rejected 166815 READS because READLEN < 1
Read 166815 spots for SRR11699265.sra
Written 166815 spots for SRR11699265.sra
Rejected 166824 READS because READLEN < 1
Read 166824 spots for SRR11699265.sra
Written 166824 spots for SRR11699265.sra
SRR ids: ['SRR11699265.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_ezlpexct
SRR11699265.sra spots: 3336309
blocks: [[1, 166815], [166816, 333630], [333631, 500445], [500446, 667260], [667261, 834075], [834076, 1000890], [1000891, 1167705], [1167706, 1334520], [1334521, 1501335], [1501336, 1668150], [1668151, 1834965], [1834966, 2001780], [2001781, 2168595], [2168596, 2335410], [2335411, 2502225], [2502226, 2669040], [2669041, 2835855], [2835856, 3002670], [3002671, 3169485], [3169486, 3336309]]
SRR11699265 file size 633163
SRR11699265 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR11699265 SRR11699265_1.fastq
Input file:	SRR11699265_1.fastq
trimmed:	SRR11699265-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	20
Wed Jun 18 05:29:04 2025 >> started

Wed Jun 18 05:29:05 2025 >> done (1.465s)
3336309 reads processed; of these:
 618131 (18.53%) short reads filtered out after trimming by size control
   3514 ( 0.11%) empty reads filtered out after trimming by size control
2714664 (81.37%) reads available; of these:
2653643 (97.75%) trimmed reads available after processing
  61021 ( 2.25%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  76388	  2.81%
 19	 120842	  4.45%
 20	 131410	  4.84%
 21	 188116	  6.93%
 22	 335084	 12.34%
 23	 116480	  4.29%
 24	 258472	  9.52%
 25	 145815	  5.37%
 26	  91180	  3.36%
 27	 126135	  4.65%
 28	  78432	  2.89%
 29	  83183	  3.06%
 30	 112726	  4.15%
 31	  86883	  3.20%
 32	  83815	  3.09%
 33	  84746	  3.12%
 34	  96651	  3.56%
 35	 124829	  4.60%
 36	  87208	  3.21%
 37	  49996	  1.84%
 38	  38514	  1.42%
 39	  33825	  1.25%
 40	  25508	  0.94%
 41	  32671	  1.20%
 42	  18740	  0.69%
 43	  15730	  0.58%
 44	  10258	  0.38%
 45	      0	  0.00%
 46	      0	  0.00%
 47	      0	  0.00%
 48	      0	  0.00%
 49	      0	  0.00%
 50	      0	  0.00%
 51	      0	  0.00%
 52	      0	  0.00%
 53	      0	  0.00%
 54	      0	  0.00%
 55	      0	  0.00%
 56	      0	  0.00%
 57	      0	  0.00%
 58	      0	  0.00%
 59	      0	  0.00%
 60	      0	  0.00%
 61	      0	  0.00%
 62	      0	  0.00%
 63	      0	  0.00%
 64	      0	  0.00%
 65	      0	  0.00%
 66	      0	  0.00%
 67	      0	  0.00%
 68	      0	  0.00%
 69	      0	  0.00%
 70	      0	  0.00%
 71	      0	  0.00%
 72	      0	  0.00%
 73	      0	  0.00%
 74	      1	  0.00%
 75	      5	  0.00%
 76	  61021	  2.25%
2714664 reads passed initial QC


criterion=sequence-density
sequence-density=4.08
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=14
prefix-density=0.01
prefix-fanout=1.0
sequence=TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCTCACCCGAAAGACCCGGGTT


criterion=fanout-score
sequence-density=0.21
sequence-density-rank=21
fanout-score=14.85
fanout-score-rank=1
prefix-density=2.09
prefix-fanout=1.5
sequence=GTCAGGATAGCTCAGTTGGTAGAGCAGAGGACTG
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	Jun 18 05:29:16
                             Started mapping on |	Jun 18 05:29:16
                                    Finished on |	Jun 18 05:29:27
       Mapping speed, Million of reads per hour |	888.44

                          Number of input reads |	2714664
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	235382
                        Uniquely mapped reads % |	8.67%
                          Average mapped length |	24.40
                       Number of splices: Total |	5710
            Number of splices: Annotated (sjdb) |	2725
                       Number of splices: GT/AG |	5243
                       Number of splices: GC/AG |	286
                       Number of splices: AT/AC |	2
               Number of splices: Non-canonical |	179
                      Mismatch rate per base, % |	1.19%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.54
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.22
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	280288
             % of reads mapped to multiple loci |	10.32%
        Number of reads mapped to too many loci |	629852
             % of reads mapped to too many loci |	23.20%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.32%
                     % of reads unmapped: other |	51.49%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	2198994	2198994	2198994
N_multimapping	280288	280288	280288
N_noFeature	190024	196794	227283
N_ambiguous	1357	17	16
UnstrandedReadsAssigned:44001 PositiveStrandReadsAssigned:38571 NegativeStrandReadsAssigned:8083
Dataset is classified unstranded
MeadianReadLen=25 20thPercentileLength=22 echo kmer=19
SRR11699265 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 72,539
[index] number of k-mers: 56,563,136
[index] number of equivalence classes: 414,827
[quant] running in single-end mode
[quant] will process file 1: SRR11699265-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 2,714,664 reads, 547,142 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 889 rounds

  72540 SRR11699265.ke.tsv
  44303 SRR11699265.se.tsv
 116843 total
==> SRR11699265.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Zm00001eb096110_T001	2067	1968	0	0
Zm00001eb321680_T001	3417	3318	1	0.605314
Zm00001eb323640_T001	3273	3174	0	0
Zm00001eb314140_T001	893	794	0	0
Zm00001eb080260_T001	3529	3430	0	0
Zm00001eb026490_T001	2433	2334	1	0.860511
Zm00001eb026490_T002	2229	2130	0	0
Zm00001eb026490_T003	2220	2121	0	0
Zm00001eb281360_T001	2412	2313	0	0

==> SRR11699265.se.tsv <==
Zm00001eb015280	0
Zm00001eb000610	0
Zm00001eb033210	1
Zm00001eb044610	0
Zm00001eb014360	0
Zm00001eb051320	0
Zm00001eb021860	0
Zm00001eb011690	0
Zm00001eb016840	0
Zm00001eb052060	0
SRR11699265 completed mapping pipeline successfully
