Starting /dee2/code/volunteer_pipeline.sh SRR11699274
    current disk space = 2470610673664
    free memory = 1581772576 
SRR11699274 SRAfilesize
3d3fbaba2b9ff17827eddeaee0671c12  SRR11699274.sra
SRR11699274.sra file validated
SRR11699274 is single end
SRR11699274 is conventional basespace
SRR11699274 read1 length is 76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR11699274_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	76
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.96875	32.0	32.0	32.0	32.0	32.0
2	31.235	32.0	32.0	32.0	32.0	32.0
3	31.42325	32.0	32.0	32.0	32.0	32.0
4	31.42075	32.0	32.0	32.0	32.0	32.0
5	31.49925	32.0	32.0	32.0	32.0	32.0
6	34.94525	36.0	36.0	36.0	36.0	36.0
7	35.04425	36.0	36.0	36.0	36.0	36.0
8	35.141	36.0	36.0	36.0	36.0	36.0
9	34.69225	36.0	36.0	36.0	32.0	36.0
10-11	34.9705	36.0	36.0	36.0	36.0	36.0
12-13	35.036625	36.0	36.0	36.0	36.0	36.0
14-15	34.86275	36.0	36.0	36.0	34.0	36.0
16-17	34.988375000000005	36.0	36.0	36.0	36.0	36.0
18-19	35.107124999999996	36.0	36.0	36.0	36.0	36.0
20-21	34.87587499999999	36.0	36.0	36.0	34.0	36.0
22-23	34.81725	36.0	36.0	36.0	34.0	36.0
24-25	34.90925	36.0	36.0	36.0	36.0	36.0
26-27	34.809875	36.0	36.0	36.0	34.0	36.0
28-29	34.821625	36.0	36.0	36.0	34.0	36.0
30-31	34.793875	36.0	36.0	36.0	34.0	36.0
32-33	34.862125	36.0	36.0	36.0	34.0	36.0
34-35	34.813625	36.0	36.0	36.0	36.0	36.0
36-37	34.78275	36.0	36.0	36.0	36.0	36.0
38-39	34.7415	36.0	36.0	36.0	32.0	36.0
40-41	34.838875	36.0	36.0	36.0	36.0	36.0
42-43	34.7795	36.0	36.0	36.0	34.0	36.0
44-45	34.75075	36.0	36.0	36.0	32.0	36.0
46-47	34.732	36.0	36.0	36.0	32.0	36.0
48-49	34.61175	36.0	36.0	36.0	32.0	36.0
50-51	34.48625	36.0	36.0	36.0	32.0	36.0
52-53	34.459	36.0	36.0	36.0	32.0	36.0
54-55	34.04675	36.0	36.0	36.0	29.5	36.0
56-57	33.495875	36.0	36.0	36.0	27.0	36.0
58-59	33.451	36.0	36.0	36.0	27.0	36.0
60-61	33.385999999999996	36.0	36.0	36.0	27.0	36.0
62-63	33.148624999999996	36.0	34.0	36.0	27.0	36.0
64-65	32.967375000000004	36.0	32.0	36.0	27.0	36.0
66-67	32.537125	36.0	32.0	36.0	27.0	36.0
68-69	32.39025	36.0	32.0	36.0	20.5	36.0
70-71	32.556124999999994	36.0	32.0	36.0	17.5	36.0
72-73	32.226625	36.0	32.0	36.0	14.0	36.0
74-75	32.796875	36.0	32.0	36.0	14.0	36.0
76	30.2045	32.0	27.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	2.0
23	3.0
24	9.0
25	15.0
26	39.0
27	47.0
28	65.0
29	109.0
30	125.0
31	139.0
32	225.0
33	391.0
34	1025.0
35	1804.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.275	29.075	25.825	18.825
2	25.3	25.2	23.925	25.575
3	25.95	29.375	16.25	28.425
4	30.3	30.025000000000002	19.0	20.674999999999997
5	20.625	26.974999999999998	25.674999999999997	26.724999999999998
6	23.275000000000002	29.075	28.575	19.075
7	36.925000000000004	31.574999999999996	17.525	13.975000000000001
8	20.200000000000003	23.625	37.425000000000004	18.75
9	23.625	38.725	16.525000000000002	21.125
10-11	32.6625	26.825	23.200000000000003	17.3125
12-13	20.4375	20.5	27.437499999999996	31.624999999999996
14-15	30.675	40.975	16.075	12.275
16-17	23.4375	22.35	34.5625	19.650000000000002
18-19	38.837500000000006	23.3125	24.099999999999998	13.750000000000002
20-21	23.4625	33.2375	26.25	17.05
22-23	33.804225528191026	39.70496312039005	13.064133016627078	13.42667833479185
24-25	30.0375	37.9625	15.0875	16.9125
26-27	28.325	32.3125	19.162499999999998	20.200000000000003
28-29	29.5	27.975	17.0	25.525
30-31	34.325	32.4625	10.7125	22.5
32-33	28.075	35.525	14.787500000000001	21.6125
34-35	30.337500000000002	31.025000000000002	15.875	22.7625
36-37	27.150000000000002	34.137499999999996	13.100000000000001	25.6125
38-39	25.637500000000003	33.2625	13.750000000000002	27.35
40-41	25.674999999999997	31.112499999999997	15.3625	27.85
42-43	28.6125	28.712500000000002	17.675	25.0
44-45	22.8375	33.537499999999994	15.25	28.375
46-47	20.599999999999998	31.7	14.5875	33.1125
48-49	16.625	27.775	17.125	38.475
50-51	17.724999999999998	26.200000000000003	18.4375	37.6375
52-53	19.564945618202277	24.0780097512189	21.115139392424055	35.24190523815477
54-55	19.400000000000002	24.575	21.125	34.9
56-57	15.9125	25.837500000000002	20.8	37.45
58-59	18.1375	22.6375	21.25	37.974999999999994
60-61	18.575	22.75	23.75	34.925
62-63	19.6375	25.112499999999997	22.650000000000002	32.6
64-65	18.6125	21.224999999999998	25.124999999999996	35.0375
66-67	19.7375	16.35	28.675	35.2375
68-69	18.1125	17.5125	30.2375	34.137499999999996
70-71	22.640330041255158	15.589448681085136	31.17889736217027	30.591323915489436
72-73	23.1375	15.937499999999998	29.2	31.724999999999998
74-75	22.175	14.1625	31.5	32.1625
76	19.225	10.65	36.475	33.650000000000006
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	2.0
40	5.5
41	9.5
42	12.0
43	24.5
44	44.5
45	83.5
46	115.0
47	154.0
48	312.0
49	736.5
50	1042.0
51	799.0
52	523.0
53	474.5
54	459.0
55	381.5
56	231.0
57	107.0
58	56.0
59	52.0
60	31.0
61	8.5
62	3.0
63	3.5
64	7.0
65	5.5
66	1.0
67	1.0
68	0.5
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0125
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0125
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0125
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.68328445747801	52.325
2	5.40427314620863	6.45
3	2.3041474654377883	4.125
4	1.1730205278592376	2.8000000000000003
5	0.8378718056137411	2.5
6	0.41893590280687054	1.5
7	0.4608294930875576	1.925
8	0.12568077084206117	0.6
9	0.0837871805613741	0.44999999999999996
>10	1.3824884792626728	19.975
>50	0.0837871805613741	3.9
>100	0.04189359028068705	3.45
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCC	138	3.45	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCA	87	2.175	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAAC	69	1.725	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	49	1.225	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCTCAGTAGCTCAGTGGTAGAGCGGTCGGCTGAGATCGGAAGAGCACAC	46	1.15	No Hit
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG	44	1.0999999999999999	TruSeq Adapter, Index 7 (97% over 36bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	43	1.075	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACT	40	1.0	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTCAGTAGCTCAGTGGTAGAGCGGTCGGCTAGATCGGAAGAGCACACG	40	1.0	No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTGAGATCGGAAGAGCAC	39	0.975	No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCAGATCGGAAGAGCACAC	37	0.9249999999999999	No Hit
CTAACGAACGAACGATTTGAAGATCGGAAGAGCACACGTCTGAACTCCAG	31	0.775	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCGTTGTAGTCTAGCTGGTCAGGATACTCAGATCGGAAGAGCACACGTC	29	0.7250000000000001	No Hit
GGGGATGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT	27	0.675	TruSeq Adapter, Index 1 (97% over 35bp)
TAATTCATGATCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	26	0.65	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAG	25	0.625	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCTAGATCGGAAGAGCA	24	0.6	No Hit
TAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCA	23	0.575	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACT	22	0.5499999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGT	22	0.5499999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTC	22	0.5499999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGC	18	0.44999999999999996	TruSeq Adapter, Index 7 (97% over 36bp)
ACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC	17	0.42500000000000004	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCAGATCGGAAGAGCACACGTC	16	0.4	No Hit
AACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGT	16	0.4	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	16	0.4	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC	15	0.375	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCAGATCGGAAGAGCACACG	15	0.375	No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTAGATCGGAAGAGCACA	14	0.35000000000000003	No Hit
TCCTCAGTAGCTCAGTGGTAGAGCGGTCGGCTGTAGATCGGAAGAGCACA	13	0.325	No Hit
TAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG	13	0.325	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	13	0.325	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCCTAACGAACGAACGATTTGAAGATCGGAAGAGCACACGTCTGAACTC	12	0.3	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGATGTAGCGCAGCTTGGTAGCGCTTTTGTTTTAGATCGGAAGAGCAC	12	0.3	No Hit
CGACACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACT	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGT	9	0.22499999999999998	No Hit
TATGCAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGA	9	0.22499999999999998	No Hit
GCCCCCATCGTCTAGTGGTTCAGGACATCTCTCTTAGATCGGAAGAGCAC	8	0.2	No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTGCTCTTAGATCGGAAGAGCAC	8	0.2	No Hit
GATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGCAGA	8	0.2	TruSeq Adapter, Index 7 (97% over 36bp)
CACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACTCCA	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGGCTATAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTG	7	0.17500000000000002	TruSeq Adapter, Index 7 (97% over 36bp)
TCAAAAGAGGAAAGGCTTGCGGTGGATACCTAGGCACCCAGAGATCGGAA	7	0.17500000000000002	No Hit
CGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCGAGCGTAGTTCAGTGGTAAAACATCTCCTTGCCAAGGAGAGATCGGAA	7	0.17500000000000002	No Hit
TATGCAGCTGAGGCATCCTAACGAACGAACGATTTGAAAGATCGGAAGAG	7	0.17500000000000002	No Hit
AACGAACGAACGATTTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAATTCATGATCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTAGATCGGAAGAGCACA	7	0.17500000000000002	No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCTAGATCGGAAGAGCACAC	7	0.17500000000000002	No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTGTAGATCGGAAGAGCA	7	0.17500000000000002	No Hit
CAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGATTGTAGTTCAATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGC	6	0.15	TruSeq Adapter, Index 12 (97% over 35bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCCAGATCGGAAGAGCACA	6	0.15	No Hit
GGGTGTTTGGTCTAGTGGTATGATTCTCGCAGATCGGAAGAGCACACGTC	6	0.15	No Hit
GGAGGTCCTGCGGCAAAATAGCTCGATGCCAGAATAGATCGGAAGAGCAC	6	0.15	No Hit
GGAGAGATGGCTGAGTGGACTAAAGCGGCGGATTAGATCGGAAGAGCACA	6	0.15	No Hit
CCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTC	6	0.15	No Hit
ATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGCAGAT	6	0.15	TruSeq Adapter, Index 7 (97% over 36bp)
ACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACAC	5	0.125	No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACTCAGATCGGAAGAGCACACGTC	5	0.125	No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGAGATCGGAAGAGCACACG	5	0.125	No Hit
CGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT	5	0.125	TruSeq Adapter, Index 1 (97% over 35bp)
TGCAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGC	5	0.125	No Hit
CAGCTGAGGCATCCTAACGAACGAACGATTTGAAAGATCGGAAGAGCACA	5	0.125	No Hit
GCCCCCATCGTCTAGTGGTTCAGGACATCTCTCTTTAGATCGGAAGAGCA	5	0.125	No Hit
TATTCTGGTGTCCTAGGCGTAGAGGAACAGATCGGAAGAGCACACGTCTG	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCGGATGTAGCCAAGTGGAAGATCGGAAGAGCACACGTCTGAACTCCAG	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACTCC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTGAGATCGGAAGAGCAC	5	0.125	No Hit
TAACGAACGAACGATTTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGAGATCGGAAGAGCACACGT	5	0.125	No Hit
GGGCCTGTAGCTCAGAGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTAGATCGGAAGAGC	5	0.125	No Hit
AGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCAC	5	0.125	No Hit
CCTGCGGCAAAATAGCTCGATGCCAGAATAGATCGGAAGAGCACACGTCT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGATGTAGCGCAGCTTGGTAGCGCTTTTGTTTAGATCGGAAGAGCACA	5	0.125	No Hit
TATTCTGGTGTCCTAGGCGTAGAGGAACCACAAGATCGGAAGAGCACACG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.05	0.0	0.0	0.0	0.0
3	0.05	0.0	0.0	0.0	0.0
4	0.075	0.0	0.0	0.0	0.0
5	0.075	0.0	0.0	0.0	0.0
6	0.125	0.0	0.0	0.0	0.0
7	0.15	0.0	0.0	0.0	0.0
8	0.25	0.0	0.0	0.0	0.0
9	0.55	0.0	0.0	0.0	0.0
10	1.0	0.0	0.0	0.0	0.0
11	1.1	0.0	0.0	0.0	0.0
12	1.4	0.0	0.0	0.0	0.0
13	2.45	0.0	0.0	0.0	0.0
14	4.525	0.0	0.0	0.0	0.0
15	6.375	0.0	0.0	0.0	0.0
16	8.025	0.0	0.0	0.0	0.0
17	10.95	0.0	0.0	0.0	0.0
18	13.7	0.0	0.0	0.0	0.0
19	16.025	0.0	0.0	0.0	0.0
20	18.95	0.0	0.0	0.0	0.0
21	22.525	0.0	0.0	0.0	0.0
22	28.175	0.0	0.0	0.0	0.0
23	35.075	0.0	0.0	0.0	0.0
24	38.45	0.0	0.0	0.0	0.0
25	45.3	0.0	0.0	0.0	0.0
26	49.0	0.0	0.0	0.0	0.0
27	51.25	0.0	0.0	0.0	0.0
28	54.875	0.0	0.0	0.0	0.0
29	57.125	0.0	0.0	0.0	0.0
30	59.925	0.0	0.0	0.0	0.0
31	64.175	0.0	0.0	0.0	0.0
32	66.75	0.0	0.0	0.0	0.0
33	70.275	0.0	0.0	0.0	0.0
34	75.375	0.0	0.0	0.0	0.0
35	78.875	0.0	0.0	0.0	0.0
36	82.9	0.0	0.0	0.0	0.0
37	86.275	0.0	0.0	0.0	0.0
38	87.75	0.0	0.0	0.0	0.0
39	88.9	0.0	0.0	0.0	0.0
40	90.15	0.0	0.0	0.0	0.0
41	90.9	0.0	0.0	0.0	0.0
42	92.025	0.0	0.0	0.0	0.0
43	92.85	0.0	0.0	0.0	0.0
44	93.45	0.0	0.0	0.0	0.0
45	93.725	0.0	0.0	0.0	0.0
46	94.175	0.0	0.0	0.0	0.0
47	94.425	0.0	0.0	0.0	0.0
48	94.65	0.0	0.0	0.0	0.0
49	94.925	0.0	0.0	0.0	0.0
50	95.25	0.0	0.0	0.0	0.0
51	95.275	0.0	0.0	0.0	0.0
52	95.325	0.0	0.0	0.0	0.0
53	95.425	0.0	0.0	0.0	0.0
54	95.425	0.0	0.0	0.0	0.0
55	95.45	0.0	0.0	0.0	0.0
56	95.475	0.0	0.0	0.0	0.0
57	95.55	0.0	0.0	0.0	0.0
58	95.55	0.0	0.0	0.0	0.0
59	95.575	0.0	0.0	0.0	0.0
60	95.575	0.0	0.0	0.0	0.0
61	95.575	0.0	0.0	0.0	0.0
62	95.575	0.0	0.0	0.0	0.0
63	95.575	0.0	0.0	0.0	0.0
64	95.575	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAAGAT	15	0.0020658788	70.00001	12
CCCAGAT	15	0.0020658788	70.00001	31
AGCTCAA	15	0.0020658788	70.00001	9
GCCCAGA	15	0.0020658788	70.00001	30
TTGTAGT	60	0.0	70.00001	5
CGCCCAG	15	0.0020658788	70.00001	29
AGTGGTA	15	0.0020658788	70.00001	14
CCGCCCA	15	0.0020658788	70.00001	28
GGATTGT	30	7.878043E-8	70.00001	2
GGTCAGA	30	7.878043E-8	70.00001	18
GTTCAAT	30	7.878043E-8	70.00001	10
GATTGTA	30	7.878043E-8	70.00001	3
TAGTTCA	30	7.878043E-8	70.00001	8
ATTGTAG	30	7.878043E-8	70.00001	4
TGTAGCT	30	7.878043E-8	70.00001	6
GGGATTG	30	7.878043E-8	70.00001	1
GGGATGT	35	2.6138878E-9	70.00001	2
GTCTAGT	15	0.0020658788	70.00001	10
AGTTCAA	30	7.878043E-8	70.00001	9
GCTCAGA	15	0.0020658788	70.00001	10
>>END_MODULE
Rejected 272228 READS because READLEN < 1
Read 272228 spots for SRR11699274.sra
Written 272228 spots for SRR11699274.sra
Rejected 272228 READS because READLEN < 1
Read 272228 spots for SRR11699274.sra
Written 272228 spots for SRR11699274.sra
Rejected 272228 READS because READLEN < 1
Read 272228 spots for SRR11699274.sra
Written 272228 spots for SRR11699274.sra
Rejected 272228 READS because READLEN < 1
Read 272228 spots for SRR11699274.sra
Written 272228 spots for SRR11699274.sra
Rejected 272228 READS because READLEN < 1
Read 272228 spots for SRR11699274.sra
Written 272228 spots for SRR11699274.sra
Rejected 272228 READS because READLEN < 1
Read 272228 spots for SRR11699274.sra
Written 272228 spots for SRR11699274.sra
Rejected 272228 READS because READLEN < 1
Read 272228 spots for SRR11699274.sra
Written 272228 spots for SRR11699274.sra
Rejected 272228 READS because READLEN < 1
Read 272228 spots for SRR11699274.sra
Written 272228 spots for SRR11699274.sra
Rejected 272228 READS because READLEN < 1
Read 272228 spots for SRR11699274.sra
Written 272228 spots for SRR11699274.sra
Rejected 272228 READS because READLEN < 1
Read 272228 spots for SRR11699274.sra
Written 272228 spots for SRR11699274.sra
Rejected 272228 READS because READLEN < 1
Read 272228 spots for SRR11699274.sra
Written 272228 spots for SRR11699274.sra
Rejected 272228 READS because READLEN < 1
Read 272228 spots for SRR11699274.sra
Written 272228 spots for SRR11699274.sra
Rejected 272228 READS because READLEN < 1
Read 272228 spots for SRR11699274.sra
Written 272228 spots for SRR11699274.sra
Rejected 272233 READS because READLEN < 1
Read 272233 spots for SRR11699274.sra
Written 272233 spots for SRR11699274.sra
Rejected 272228 READS because READLEN < 1
Read 272228 spots for SRR11699274.sra
Written 272228 spots for SRR11699274.sra
Rejected 272228 READS because READLEN < 1
Read 272228 spots for SRR11699274.sra
Written 272228 spots for SRR11699274.sra
Rejected 272228 READS because READLEN < 1
Read 272228 spots for SRR11699274.sra
Written 272228 spots for SRR11699274.sra
Rejected 272228 READS because READLEN < 1
Read 272228 spots for SRR11699274.sra
Written 272228 spots for SRR11699274.sra
Rejected 272228 READS because READLEN < 1
Read 272228 spots for SRR11699274.sra
Written 272228 spots for SRR11699274.sra
Rejected 272228 READS because READLEN < 1
Read 272228 spots for SRR11699274.sra
Written 272228 spots for SRR11699274.sra
SRR ids: ['SRR11699274.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_5l43frg7
SRR11699274.sra spots: 5444565
blocks: [[1, 272228], [272229, 544456], [544457, 816684], [816685, 1088912], [1088913, 1361140], [1361141, 1633368], [1633369, 1905596], [1905597, 2177824], [2177825, 2450052], [2450053, 2722280], [2722281, 2994508], [2994509, 3266736], [3266737, 3538964], [3538965, 3811192], [3811193, 4083420], [4083421, 4355648], [4355649, 4627876], [4627877, 4900104], [4900105, 5172332], [5172333, 5444565]]
SRR11699274 file size 1034637
SRR11699274 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR11699274 SRR11699274_1.fastq
Input file:	SRR11699274_1.fastq
trimmed:	SRR11699274-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	20
Wed Jun 18 07:07:57 2025 >> started

Wed Jun 18 07:07:59 2025 >> done (2.407s)
5444565 reads processed; of these:
1008923 (18.53%) short reads filtered out after trimming by size control
   4132 ( 0.08%) empty reads filtered out after trimming by size control
4431510 (81.39%) reads available; of these:
4319880 (97.48%) trimmed reads available after processing
 111630 ( 2.52%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	 120003	  2.71%
 19	 180283	  4.07%
 20	 199311	  4.50%
 21	 300328	  6.78%
 22	 369841	  8.35%
 23	 181127	  4.09%
 24	 400199	  9.03%
 25	 199657	  4.51%
 26	 131123	  2.96%
 27	 172643	  3.90%
 28	 116187	  2.62%
 29	 132526	  2.99%
 30	 216664	  4.89%
 31	 147882	  3.34%
 32	 211005	  4.76%
 33	 317513	  7.16%
 34	 197431	  4.46%
 35	 194053	  4.38%
 36	 155243	  3.50%
 37	  78601	  1.77%
 38	  59586	  1.34%
 39	  51658	  1.17%
 40	  43477	  0.98%
 41	  58271	  1.31%
 42	  35579	  0.80%
 43	  29679	  0.67%
 44	  20003	  0.45%
 45	      0	  0.00%
 46	      0	  0.00%
 47	      0	  0.00%
 48	      0	  0.00%
 49	      0	  0.00%
 50	      0	  0.00%
 51	      0	  0.00%
 52	      0	  0.00%
 53	      0	  0.00%
 54	      0	  0.00%
 55	      0	  0.00%
 56	      0	  0.00%
 57	      0	  0.00%
 58	      0	  0.00%
 59	      0	  0.00%
 60	      0	  0.00%
 61	      0	  0.00%
 62	      0	  0.00%
 63	      0	  0.00%
 64	      0	  0.00%
 65	      0	  0.00%
 66	      0	  0.00%
 67	      0	  0.00%
 68	      0	  0.00%
 69	      0	  0.00%
 70	      0	  0.00%
 71	      0	  0.00%
 72	      0	  0.00%
 73	      0	  0.00%
 74	      0	  0.00%
 75	      7	  0.00%
 76	 111630	  2.52%
4431510 reads passed initial QC


criterion=sequence-density
sequence-density=5.94
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=16
prefix-density=0.00
prefix-fanout=1.0
sequence=TCCTCAGTAGCTCAGTGGTAGAGCGGTCGGCTGTTAACTGACT


criterion=fanout-score
sequence-density=2.06
sequence-density-rank=4
fanout-score=17.09
fanout-score-rank=1
prefix-density=2.10
prefix-fanout=16.8
sequence=AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGCAGATCTCGTATGCCGTCTTCTGCTTGA
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    1 (100.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    0 (0.00%) aligned >1 times
0.00% overall alignment rate
Adapter seq not found in reference. Now shuffling file before clipping
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -t 20 -x TCCTCAGTAGCTCAGTGGTAGAGCGGTCGGCTGTTAACTGACT -o SRR11699274 -
Input file:	STDIN
trimmed:	SRR11699274-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	TCCTCAGTAGCTCAGTGGTAGAGCGGTCGGCTGTTAACTGACT
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	3
-- number of concurrent threads (-t):	20
Wed Jun 18 07:08:05 2025 >> started

Wed Jun 18 07:08:07 2025 >> done (1.895s)
2954340 reads processed; of these:
   7254 ( 0.25%) short reads filtered out after trimming by size control
 177661 ( 6.01%) empty reads filtered out after trimming by size control
2769425 (93.74%) reads available; of these:
  49650 ( 1.79%) trimmed reads available after processing
2719775 (98.21%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  80406	  2.90%
 19	 119523	  4.32%
 20	 132271	  4.78%
 21	 200926	  7.26%
 22	 246312	  8.89%
 23	 120725	  4.36%
 24	 265527	  9.59%
 25	 133332	  4.81%
 26	  88544	  3.20%
 27	 117377	  4.24%
 28	  76322	  2.76%
 29	  87067	  3.14%
 30	 140362	  5.07%
 31	  92796	  3.35%
 32	  94368	  3.41%
 33	 107337	  3.88%
 34	 118267	  4.27%
 35	 125186	  4.52%
 36	 100711	  3.64%
 37	  52351	  1.89%
 38	  40063	  1.45%
 39	  34840	  1.26%
 40	  28987	  1.05%
 41	  38208	  1.38%
 42	  22375	  0.81%
 43	  17968	  0.65%
 44	  12892	  0.47%
 45	      0	  0.00%
 46	      0	  0.00%
 47	      0	  0.00%
 48	      0	  0.00%
 49	      0	  0.00%
 50	      0	  0.00%
 51	      0	  0.00%
 52	      0	  0.00%
 53	      0	  0.00%
 54	      0	  0.00%
 55	      0	  0.00%
 56	      0	  0.00%
 57	      0	  0.00%
 58	      0	  0.00%
 59	      0	  0.00%
 60	      0	  0.00%
 61	      0	  0.00%
 62	      0	  0.00%
 63	      0	  0.00%
 64	      0	  0.00%
 65	      0	  0.00%
 66	      0	  0.00%
 67	      0	  0.00%
 68	      5	  0.00%
 69	      0	  0.00%
 70	      1	  0.00%
 71	     15	  0.00%
 72	     46	  0.00%
 73	  11150	  0.40%
 74	      0	  0.00%
 75	      5	  0.00%
 76	  63160	  2.28%


criterion=sequence-density
sequence-density=5.23
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=15
prefix-density=0.02
prefix-fanout=1.0
sequence=TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCTCACCCGAAAGACCCGGGTT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=35
fanout-score=65.86
fanout-score-rank=1
prefix-density=0.39
prefix-fanout=1.0
sequence=CCTCGTGTTTAGTTGCCACTATGAGTTTGGAACCCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGATCGGAA
                                 Started job on |	Jun 18 07:08:32
                             Started mapping on |	Jun 18 07:08:32
                                    Finished on |	Jun 18 07:08:43
       Mapping speed, Million of reads per hour |	1389.79

                          Number of input reads |	4246595
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	381050
                        Uniquely mapped reads % |	8.97%
                          Average mapped length |	25.73
                       Number of splices: Total |	8795
            Number of splices: Annotated (sjdb) |	4446
                       Number of splices: GT/AG |	8031
                       Number of splices: GC/AG |	511
                       Number of splices: AT/AC |	4
               Number of splices: Non-canonical |	249
                      Mismatch rate per base, % |	1.29%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.30
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.31
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	532439
             % of reads mapped to multiple loci |	12.54%
        Number of reads mapped to too many loci |	1023439
             % of reads mapped to too many loci |	24.10%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.47%
                     % of reads unmapped: other |	47.92%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	3333106	3333106	3333106
N_multimapping	532439	532439	532439
N_noFeature	301937	311981	368634
N_ambiguous	2425	39	22
UnstrandedReadsAssigned:76688 PositiveStrandReadsAssigned:69030 NegativeStrandReadsAssigned:12394
Dataset is classified positive stranded
MeadianReadLen=26 20thPercentileLength=22 echo kmer=19
SRR11699274 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 72,539
[index] number of k-mers: 56,563,136
[index] number of equivalence classes: 414,827
[quant] running in single-end mode
[quant] will process file 1: SRR11699274-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 4,246,595 reads, 502,765 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 882 rounds

  72540 SRR11699274.ke.tsv
  44303 SRR11699274.se.tsv
 116843 total
==> SRR11699274.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Zm00001eb096110_T001	2067	1968	2	2.04948
Zm00001eb321680_T001	3417	3318	1	0.607804
Zm00001eb323640_T001	3273	3174	0	0
Zm00001eb314140_T001	893	794	0	0
Zm00001eb080260_T001	3529	3430	1	0.587957
Zm00001eb026490_T001	2433	2334	0	0
Zm00001eb026490_T002	2229	2130	0	0
Zm00001eb026490_T003	2220	2121	0	0
Zm00001eb281360_T001	2412	2313	0	0

==> SRR11699274.se.tsv <==
Zm00001eb015280	0
Zm00001eb000610	0
Zm00001eb033210	0
Zm00001eb044610	0
Zm00001eb014360	0
Zm00001eb051320	0
Zm00001eb021860	0
Zm00001eb011690	0
Zm00001eb016840	0
Zm00001eb052060	0
SRR11699274 completed mapping pipeline successfully
