Starting /dee2/code/volunteer_pipeline.sh SRR11699361
    current disk space = 2462086287360
    free memory = 1558877544 
SRR11699361 SRAfilesize
0736f1eee9875b4ead32ff1ba367dca2  SRR11699361.sra
SRR11699361.sra file validated
SRR11699361 is single end
SRR11699361 is conventional basespace
SRR11699361 read1 length is 86 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR11699361_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	675
Sequences flagged as poor quality	0
Sequence length	86
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.54074074074074	32.0	32.0	32.0	32.0	32.0
2	30.977777777777778	32.0	32.0	32.0	32.0	32.0
3	31.36740740740741	32.0	32.0	32.0	32.0	32.0
4	31.59259259259259	32.0	32.0	32.0	32.0	32.0
5	31.69037037037037	32.0	32.0	32.0	32.0	32.0
6	34.2	36.0	36.0	36.0	32.0	36.0
7	34.6162962962963	36.0	36.0	36.0	32.0	36.0
8	35.14074074074074	36.0	36.0	36.0	36.0	36.0
9	33.641481481481485	36.0	36.0	36.0	21.0	36.0
10-11	34.494814814814816	36.0	36.0	36.0	34.0	36.0
12-13	34.925925925925924	36.0	36.0	36.0	34.0	36.0
14-15	33.620000000000005	36.0	34.0	36.0	28.5	36.0
16-17	34.44592592592593	36.0	36.0	36.0	32.0	36.0
18-19	34.61259259259259	36.0	36.0	36.0	34.0	36.0
20-21	34.736296296296295	36.0	36.0	36.0	32.0	36.0
22-23	34.00370370370371	36.0	36.0	36.0	32.0	36.0
24-25	33.946666666666665	36.0	36.0	36.0	26.5	36.0
26-27	34.65851851851852	36.0	36.0	36.0	34.0	36.0
28-29	34.074074074074076	36.0	36.0	36.0	29.5	36.0
30-31	33.842962962962964	36.0	36.0	36.0	26.5	36.0
32-33	34.022222222222226	36.0	36.0	36.0	32.0	36.0
34-35	34.434074074074076	36.0	36.0	36.0	32.0	36.0
36-37	33.754074074074076	36.0	36.0	36.0	26.5	36.0
38-39	34.22814814814815	36.0	36.0	36.0	32.0	36.0
40-41	32.26148148148148	36.0	31.5	36.0	23.0	36.0
42-43	25.994814814814816	29.5	14.0	36.0	14.0	36.0
44-45	31.034074074074077	36.0	25.0	36.0	17.5	36.0
46-47	33.04518518518518	36.0	36.0	36.0	14.0	36.0
48-49	33.13925925925926	36.0	36.0	36.0	17.5	36.0
50-51	32.64074074074074	36.0	34.0	36.0	14.0	36.0
52-53	32.434074074074076	36.0	34.0	36.0	14.0	36.0
54-55	32.456296296296294	36.0	34.0	36.0	17.5	36.0
56-57	32.27703703703703	36.0	34.0	36.0	17.5	36.0
58-59	32.80888888888889	36.0	34.0	36.0	17.5	36.0
60-61	31.96222222222222	36.0	32.0	36.0	17.5	36.0
62-63	32.10074074074074	36.0	32.0	36.0	14.0	36.0
64-65	32.404444444444444	36.0	34.0	36.0	20.5	36.0
66-67	31.835555555555555	36.0	32.0	36.0	14.0	36.0
68-69	32.95481481481481	36.0	32.0	36.0	24.0	36.0
70-71	33.24148148148148	36.0	36.0	36.0	24.0	36.0
72-73	31.35333333333333	36.0	29.5	36.0	14.0	36.0
74-75	20.484444444444446	14.0	14.0	34.0	14.0	36.0
76-77	26.089629629629627	29.5	14.0	36.0	14.0	36.0
78-79	30.414074074074072	36.0	29.5	36.0	14.0	36.0
80-81	31.375555555555557	36.0	32.0	36.0	14.0	36.0
82-83	32.17259259259259	36.0	32.0	36.0	14.0	36.0
84-85	32.22	36.0	34.0	36.0	14.0	36.0
86	28.651851851851852	32.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	4.0
23	7.0
24	10.0
25	14.0
26	18.0
27	19.0
28	23.0
29	25.0
30	36.0
31	45.0
32	74.0
33	159.0
34	187.0
35	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.703703703703704	74.22222222222223	7.555555555555555	6.518518518518518
2	73.77777777777777	6.518518518518518	6.222222222222222	13.481481481481481
3	6.518518518518518	75.40740740740742	9.62962962962963	8.444444444444445
4	11.703703703703704	5.9259259259259265	74.22222222222223	8.148148148148149
5	5.777777777777778	6.814814814814815	12.74074074074074	74.66666666666667
6	76.0	4.296296296296296	13.185185185185185	6.518518518518518
7	84.5925925925926	6.666666666666667	3.111111111111111	5.62962962962963
8	10.962962962962964	74.96296296296296	10.37037037037037	3.7037037037037033
9	3.851851851851852	84.88888888888889	8.148148148148149	3.111111111111111
10-11	44.96296296296297	38.88888888888889	8.814814814814815	7.333333333333333
12-13	43.03703703703704	4.444444444444445	8.0	44.51851851851852
14-15	8.74074074074074	46.59259259259259	4.074074074074074	40.59259259259259
16-17	9.25925925925926	40.44444444444444	6.888888888888889	43.407407407407405
18-19	48.66666666666667	3.5555555555555554	40.51851851851852	7.2592592592592595
20-21	5.703703703703704	8.74074074074074	42.888888888888886	42.66666666666667
22-23	43.48148148148148	46.44444444444444	4.0	6.074074074074074
24-25	9.333333333333334	44.81481481481481	6.518518518518518	39.33333333333333
26-27	8.074074074074074	8.074074074074074	42.2962962962963	41.55555555555556
28-29	9.925925925925926	46.666666666666664	4.148148148148148	39.25925925925926
30-31	44.148148148148145	11.555555555555555	38.592592592592595	5.703703703703704
32-33	10.0	42.22222222222222	3.4074074074074074	44.370370370370374
34-35	7.185185185185185	11.925925925925926	37.48148148148148	43.407407407407405
36-37	45.407407407407405	9.777777777777779	3.6296296296296298	41.18518518518518
38-39	8.296296296296296	79.85185185185185	4.222222222222222	7.629629629629629
40-41	39.7037037037037	42.51851851851852	6.962962962962963	10.814814814814815
42-43	9.851851851851851	9.62962962962963	38.370370370370374	42.148148148148145
44-45	6.7407407407407405	12.0	38.148148148148145	43.111111111111114
46-47	40.370370370370374	8.88888888888889	40.81481481481482	9.925925925925926
48-49	5.2592592592592595	42.22222222222222	42.0	10.518518518518519
50-51	38.592592592592595	8.962962962962964	7.629629629629629	44.81481481481481
52-53	40.370370370370374	7.111111111111111	7.777777777777778	44.74074074074074
54-55	3.3333333333333335	7.2592592592592595	42.888888888888886	46.51851851851852
56-57	7.555555555555555	7.777777777777778	73.85185185185185	10.814814814814815
58-59	5.333333333333334	10.222222222222223	40.074074074074076	44.370370370370374
60-61	39.7037037037037	9.851851851851851	7.555555555555555	42.888888888888886
62-63	3.4814814814814814	8.814814814814815	79.55555555555556	8.148148148148149
64-65	37.111111111111114	41.92592592592593	11.25925925925926	9.703703703703702
66-67	6.666666666666667	75.18518518518519	8.148148148148149	10.0
68-69	3.4074074074074074	78.96296296296296	12.88888888888889	4.7407407407407405
70-71	8.148148148148149	76.29629629629629	7.4074074074074066	8.148148148148149
72-73	6.962962962962963	77.48148148148148	6.37037037037037	9.185185185185185
74-75	28.074074074074073	38.44444444444444	20.0	13.481481481481481
76-77	72.0	6.074074074074074	13.185185185185185	8.74074074074074
78-79	71.11111111111111	6.222222222222222	10.962962962962964	11.703703703703704
80-81	77.25925925925927	3.7777777777777777	10.222222222222223	8.74074074074074
82-83	77.03703703703704	2.9629629629629632	12.592592592592592	7.4074074074074066
84-85	79.33333333333333	5.2592592592592595	8.962962962962964	6.444444444444445
86	78.51851851851852	8.0	9.481481481481481	4.0
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.5
31	0.5
32	0.0
33	0.0
34	1.5
35	1.5
36	0.0
37	0.5
38	1.0
39	0.5
40	0.5
41	1.0
42	3.0
43	5.0
44	6.0
45	8.0
46	10.5
47	13.5
48	29.5
49	43.0
50	42.0
51	76.5
52	233.5
53	198.0
54	26.0
55	9.0
56	4.0
57	2.0
58	1.0
59	0.5
60	1.0
61	1.0
62	0.5
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.5
73	0.5
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	675.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.40740740740741
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.48648648648648	23.703703703703706
2	5.405405405405405	2.9629629629629632
3	4.324324324324325	3.5555555555555554
4	0.0	0.0
5	0.5405405405405406	0.7407407407407408
6	0.0	0.0
7	0.0	0.0
8	0.5405405405405406	1.1851851851851851
9	0.5405405405405406	1.3333333333333335
>10	1.6216216216216217	7.4074074074074066
>50	0.0	0.0
>100	0.5405405405405406	59.111111111111114
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATG	399	59.111111111111114	TruSeq Adapter, Index 10 (97% over 36bp)
GTTCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCT	20	2.9629629629629632	TruSeq Adapter, Index 10 (97% over 36bp)
GCTGTCGGTTGCTGTCGGTTAGATCGGAAGAGCACACGTCTGAACTCCAG	19	2.814814814814815	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	11	1.6296296296296295	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCATCTCATCCCTGCGTGTCTCCGACTCAGAGATCGGAAGAGCACACGTC	9	1.3333333333333335	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATGTCGTATG	8	1.1851851851851851	TruSeq Adapter, Index 10 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATATCGTATG	5	0.7407407407407408	TruSeq Adapter, Index 10 (97% over 36bp)
AGATCGGAAGAGCACACGTCAGATCGGAAGAGCACACGTCTGAACTCCAG	3	0.4444444444444444	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT	3	0.4444444444444444	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTAT	3	0.4444444444444444	TruSeq Adapter, Index 10 (97% over 36bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGC	3	0.4444444444444444	TruSeq Adapter, Index 3 (97% over 34bp)
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC	3	0.4444444444444444	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGGGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATG	3	0.4444444444444444	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CAGAGTTCTACAGTCCGACGATCCAGAGATCGGAAGAGCACACGTCTGAA	3	0.4444444444444444	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATAGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATG	3	0.4444444444444444	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TTCAGAGTTCTACAGTCCGACGATCTTCAGAGATCGGAAGAGCACACGTC	2	0.2962962962962963	Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGAGGTCGTATG	2	0.2962962962962963	TruSeq Adapter, Index 10 (97% over 36bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATG	2	0.2962962962962963	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	2	0.2962962962962963	No Hit
CAGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTC	2	0.2962962962962963	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTAGTATG	2	0.2962962962962963	TruSeq Adapter, Index 10 (97% over 36bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATG	2	0.2962962962962963	TruSeq Adapter, Index 10 (97% over 36bp)
GATCGTAAGAGCACACGTCTTAGATCGGAAGAGCACACGTCTGAACTCCA	2	0.2962962962962963	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTAAG	2	0.2962962962962963	TruSeq Adapter, Index 10 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGAATG	2	0.2962962962962963	TruSeq Adapter, Index 10 (97% over 36bp)
GCACCAGTAGTCTAGTGGTAGAATAAGATCGGAAGAGCAACGTCTGAACT	1	0.14814814814814814	No Hit
AGATCGGAAGAGCACACCTCTGAACTCCAGTCACTCGAAGATCTCGTATG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AAGCTCGTAGTTGGACCAGATCGGAAGGGCACACGTCTGAACTCCAGTCA	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GAAACAGTAGTCTATTGTTATAATAGTACCATGCAGATATGAAGAGAACA	1	0.14814814814814814	No Hit
GCGACCCCAGGTCAGTCGGGACTACCCGCTGCGTATATCGTAAGCTCACA	1	0.14814814814814814	No Hit
AGATCGGAAGAGCACACCTCTGAACTCCAGTCACTCGAAGATCTCGAATG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CCTTCGGTTCTAGATAAACACTAGATCTTAAGAGCACACGACTGAACTCC	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AGATCGGAAGTGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGTCAGA	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CAGACTGAGAGCTCTTTCTTGAGATCGGAAGAGCACACGTCTGAATCCAG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TTCTGGCGGAGGGCACGTCTGCCTGGGCGTCACGAGATCGGAAGAGCACA	1	0.14814814814814814	No Hit
CGGCGAGAACCGACCGGCTCGACCCTACAGCCGGCGAGATCGGAAGAGCA	1	0.14814814814814814	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCAATCGAAGATCTCGTATG	1	0.14814814814814814	TruSeq Adapter, Index 3 (97% over 34bp)
CTGAGTCGGAGACACGCAGGGATGAGACGGAAGAGCACACGTCTGAACTC	1	0.14814814814814814	Illumina Multiplexing Index Sequencing Primer (95% over 24bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGTTGAA	1	0.14814814814814814	No Hit
CCAGGGAATAGCACAAACTCTAGAAAATCGGAAGCGCACACGTCTGAACT	1	0.14814814814814814	No Hit
GTTCAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCT	1	0.14814814814814814	TruSeq Adapter, Index 10 (97% over 36bp)
CCGACGATCAGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGT	1	0.14814814814814814	Illumina DpnII expression Sequencing Primer (96% over 26bp)
ACCGAATACCCTCCACGCCCAGAACCGAACGGCACACGTCTGAACTCAAG	1	0.14814814814814814	No Hit
CACGACTCTCGGCAACGGATAAGATCGGGAGAGCACACGTCTGAACTCCA	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AAGTTCTGGTCCTCTAAGATCGGAGGAGCACACGTCTGAACTCCAGTCAC	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGAGCTCGTATG	1	0.14814814814814814	TruSeq Adapter, Index 10 (97% over 36bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CTACAGTCCGACGATCCAGAGTTCTACAGTCCGACGATCAGATCGGAAGA	1	0.14814814814814814	Illumina DpnII expression Sequencing Primer (100% over 23bp)
CGACACGACTCTCGGCAACGGATAACTCGGCTCTCGAGAACGGAAGATCA	1	0.14814814814814814	No Hit
TACGTTGTAGTATAGCTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTTGTAGTCTAGCTGGCCAGGATATTTGGCTCAGATCGGAAGAGCAC	1	0.14814814814814814	No Hit
CAGGGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTC	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGTTGTAGTCTAGGTGGTCAGGATAAGATCGGAAGGGCACACGTCTGA	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ACCGAACAAGTATAGTAGAAAAAGATAGAGCAATAGAACACAACTCACAT	1	0.14814814814814814	No Hit
CAGGCGAGAGACCGGTAGCGAAGATCGGAAGAGACACGTCTGAACTCCAG	1	0.14814814814814814	No Hit
AAATATGATGTGCGATATTAGAGTAGATCGGAAGAGCACACGTCTGAATC	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGCCGTC	1	0.14814814814814814	RNA PCR Primer, Index 47 (100% over 34bp)
ACGTGAGAGATAAAAAAATAGATCAGATCGGAAGAAGCACACGTCTGAAC	1	0.14814814814814814	No Hit
GGAGTATGGTCGCAAGGCTGAAACTTAAAGGAAGATCGGAAGAGAACACG	1	0.14814814814814814	No Hit
AGATCGGAAGAGCACACGTCTGACTCCAGTCACTCGAAGATCTCGTATGC	1	0.14814814814814814	RNA PCR Primer, Index 47 (96% over 29bp)
AGAATCGGAGGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTAT	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTATCCGATGGGAACAAAGCTCA	1	0.14814814814814814	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGAAGATCTCGTATG	1	0.14814814814814814	TruSeq Adapter, Index 15 (97% over 36bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAATCTCGGAAGAGAACAAGTCTGA	1	0.14814814814814814	No Hit
GTTCAGAGACCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCT	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
ACACGTGTTCGGTCTCTTTTTAAAAAGATCGGAAGAGCACACGTCTGAAC	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCTGATAGGTAGAGCAC	1	0.14814814814814814	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCACTCACTCGAAGATGTCGTATG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AGATCGGAGGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CAGAGTTCTACAGTCCGACGATCTCAGATCGGAAGAGCACACGTCTGAAC	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCAATCTTAAACTTCTTAGACGAATCACCAGAACGGAAAACATCAATCA	1	0.14814814814814814	No Hit
AGGATCATTGCGGGGGCCAGATCGGAAGAGCACACGTCTGACTCCAGTCA	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAG	1	0.14814814814814814	TruSeq Adapter, Index 10 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAGCTCCAGTCACTCGAAGATCTCGTATG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCAGTCACTCGAAGATCTCGTATGC	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TCCGTTGTAGTCTAGCAGATCGGAAGAGCACACGTCTGACTCCAGTCACA	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ACAGTCCGACGATCGTTCTACAGTCCGACGATCGGAAGAGCACACGTCTG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GACAGACTGAGAGCTCAGATCGGAAGGGCACACGTCTGAACTCCAGTCAC	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AACCGACTCAGAACTGGTACGGACAAGGGGAGATCGGAAGTGCACACGTC	1	0.14814814814814814	No Hit
GCGACCCCAGGTCAGTCGGGAATAGCGGATGAGGAGAAGAAGATCGCACG	1	0.14814814814814814	No Hit
CGGGGTGGCCCATAGGTTGTCTGACAGGCAGATCGGAAGAGCACACGTCT	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTTGGATTTATGAAAGACGAACAACAGATCGGAAGAGCACACGTCTGA	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTTCACTCTGCAAGCAGCTGGAGGTGGGAAGAGCACACGTCTGAACTCC	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTCGTAGTTGGACCTTGGGCCGGGCCGGCCGGTCCGCCTCAAGATCGGA	1	0.14814814814814814	No Hit
GAGTTCTACAGTCCGGACGACAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATAGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATGTCCTAAG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCC	1	0.14814814814814814	TruSeq Adapter, Index 6 (97% over 36bp)
CAGTCCGACGATCCAGGGTTCTACAGTCCGACGATCAGATCGGAAGAGCA	1	0.14814814814814814	Illumina DpnII expression Sequencing Primer (95% over 23bp)
AACAGCCCCGCGTCGCACGGATTCGTAGATCGGAAGGGCACACCTCTGAA	1	0.14814814814814814	No Hit
AGATCGGAAGAGCTCACGTCTGAACTCCAGTCACTCGAAGAGCTCGTATG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AGTTCTACAGTCCGACGAACAGATCGGGAGAGCACACGTCTGAACTCCAG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ACACCACGCGGCGAGGACGGATGGAGATCGGAAGGGCACACGTCTGAACT	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GCGACCCCAGGTCAGTCGGGACTACAACCGAAGAACACACCTCTGAACAC	1	0.14814814814814814	No Hit
AAATCAGACTTAAAACTGAAACGATAAGATCTGAACACCACACTTAACAA	1	0.14814814814814814	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGGGCACACGTCTGA	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAATATCGGTAGATCTCAAGAGAAC	1	0.14814814814814814	No Hit
AAATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGAGCTCGTATG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CTTAATCTGTGAGATGTCTACAGATCGGAAGAGCACACGTCTGAACTCCG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGACCAGTCAGCCGTGTTCCGTTGACCTGTGCAGACGTCTGGAGGGTCAC	1	0.14814814814814814	No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCTAGATCGGAAGAGCACAC	1	0.14814814814814814	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGACCTCGTATG	1	0.14814814814814814	TruSeq Adapter, Index 10 (97% over 36bp)
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACTCGAAGATCTCGTATG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TCCGTTGTAGTCTAGCTGGTGAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTCTTTGATGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTCTGACACCAAGATCGGAAGAGCACACGTCTGAACTCCGGTCACAATA	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGCCTAGATCGGAAGAGCACA	1	0.14814814814814814	No Hit
GCCGTTGTAGTCTAGCTGGTCAGGATAATCTCCGAAGCGCACAAGACAAA	1	0.14814814814814814	No Hit
GTCCGACGATCTCCGATCAGAGTTCTACAGTCCGACGATCAGATCGGAAG	1	0.14814814814814814	Illumina DpnII expression Sequencing Primer (96% over 26bp)
TTTTCAAGCACAGCATGTTCAGTCAGATCGGAAGAGCACACGTCTGACTC	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAATCTCGGTAGAGCACAAGACAGC	1	0.14814814814814814	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGAAATCGTATG	1	0.14814814814814814	TruSeq Adapter, Index 10 (97% over 36bp)
TACTATGATGGTTAGATACTTGTGAGATCGGAAGGGCACACGTCTGAACT	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TCCGTTGTAGTCTAGTTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGTCCATAGCTCAGTGGTAGAAGATCGGAAGAGCACACGTTGAACTCCA	1	0.14814814814814814	No Hit
AGCCGCTCTAATACCCTCTGGTTTAGATCGGAAGAGCACACGTCGAACTC	1	0.14814814814814814	No Hit
AGATGGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AAAATAACTGCTTATTTTCGACGGAGATCGGAAGAGCCACGTCTGAACTC	1	0.14814814814814814	No Hit
AGATCGGAAGAGCACACCTCTGAACACCAGTCACTCGAAGATCTCGTATG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
AAAAAGGGACTGATCTTCTTTTCAAGATCGGAAGAGCACACGTCTGAACT	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTTGTAGTCTAGCTGGTGAGGAGAAGATCGGAAGAGCACACGTCTGA	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAGCAGGGGAAGACAAACGGAATAA	1	0.14814814814814814	No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCTTCAGTCGGAAGAGCACA	1	0.14814814814814814	No Hit
AGATCGGAAGAGCACACGTGTGAACTCCAGTCACTCGAAGACCTCGTATG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
CGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGAT	1	0.14814814814814814	TruSeq Adapter, Index 10 (97% over 36bp)
CGACACGACTCTCGGCAACGAGATCGGAAGAGCACACGTCTGAACTCCAG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCT	1	0.14814814814814814	TruSeq Adapter, Index 10 (97% over 36bp)
AGATCGGAATAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TCCTTTTTGCTCCAAGGCCCGGCCAGATCGGACGGCCAAACTACGGACCT	1	0.14814814814814814	No Hit
GTTCAGAGCACACGTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCATTGTAGTCTAGATGGTCAGGATAATCTCCTAAGAACAAACGACTCA	1	0.14814814814814814	No Hit
CGGCTTGTGGGTAAAGTGCACAAGATCGGGAGAGCACACGTCTGAACTCC	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
AGAGTTCTACAGTCCGCCGATCAGATCGGAAGAGCACACGTCTGAACTCC	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCTGTCACTCGAAGATCTCGTATG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AGATCAGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GCACCAGTGGCCTAGTGGTAGAATAAGATCGGAAGGGCACACGTCTGAAC	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
CGAGTTGGCTCGTTAAGGAAACGAAGATCGGAAGGGCACACGTCTGAACT	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AGATCGGAAGAGCACACGTCGGAACTCCAGTCACTCGAAGATCTCGTATG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TGGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCT	1	0.14814814814814814	TruSeq Adapter, Index 10 (97% over 36bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTAT	1	0.14814814814814814	TruSeq Adapter, Index 10 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATT	1	0.14814814814814814	TruSeq Adapter, Index 10 (97% over 36bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGC	1	0.14814814814814814	RNA PCR Primer, Index 47 (96% over 31bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTAGTATG	1	0.14814814814814814	TruSeq Adapter, Index 10 (97% over 36bp)
CCGCTTATACACATTGTCAGATAAGATCGGAAGGGCACACGTCTGAACTC	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATGAGGCGAAGATCGGAAGACACA	1	0.14814814814814814	No Hit
AGACCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TTCTACAGTCCGACGATCGACGATCAGATCGGAAGAGCACACGTCTGAAC	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGTTGTAGTCTAGCTGGTCAGGAGATCGGAAGAGCACACCTCTGCACT	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATTTATGAAAGACGAACAACTGCGAAAGCATTAGATCGGAAGAGCACAC	1	0.14814814814814814	No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATATTAGATCGGAAGAGCACACGTCT	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCTACCTCGTAAGAACACCA	1	0.14814814814814814	No Hit
TGAACCGGAAGATCGGAAGAGCACACTCTGAACTCCAGTCACAATAACAT	1	0.14814814814814814	No Hit
AGAGTTCTACACTCCGACGATCGATCAGATCGGAAGAGCACACGTCTGAA	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGAGTCGGAGACACGCAGGGATGAGATCGGAAGAGCACACGTCTGAACT	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGGTGGTGAGAAGATTTTTCTGACACAAGATCGGAAGAGCACACGTCT	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGGGTAAAAGCTCGTTTGATTCTGATTTCCAAGATCGGAAGGGCACA	1	0.14814814814814814	No Hit
GATCGGAAGAGCACACGTCTCAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATGTCGTAAG	1	0.14814814814814814	TruSeq Adapter, Index 10 (97% over 36bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AGATCGGAAGAGCACACGTCTTAGATCGGAAGAGCACACGTCTGAACTCC	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACCCAGTCACTCGAAGATCTCGTATGC	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTA	1	0.14814814814814814	TruSeq Adapter, Index 10 (97% over 36bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAATATCCTCTCAGCACTGGACAGC	1	0.14814814814814814	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGAAGATCTCGTATG	1	0.14814814814814814	TruSeq Adapter, Index 7 (97% over 37bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATG	1	0.14814814814814814	TruSeq Adapter, Index 10 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGACACTCGAAGATCTCGTATG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAGGAGCACACGTCTGA	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GATCCAGCCCCGCGTCGCACGGATTCGTAGATCGGAAGAGCACACGTCTG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTCAGAGTTCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGAGCT	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TCCGTTGTAGTCTAGCTGCTCAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTCTACAGTCCGACGATAGATCGGAAGAGCACACGTCTGAACTCCAGT	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTAGGCTCTAGTACGGAAGGGCA	1	0.14814814814814814	No Hit
CAGAGTTCTACAGTCCGACGATCTAGATCGGAAGAGCACACGTCTGAACT	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGACCCCAGGTCAGTCGGGACTAGCGCGTGAGATAGCTCGTATGAGCTC	1	0.14814814814814814	No Hit
AGATCGGAAGAGCACACGTCTCAGATCGGAAGAGCACACGTCTGAACTCC	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTCATCGATGTGCGAGACTCAGATCGGAAGAGCACATGTCTGAACTCCA	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
CGTAAATGCGGTCTTTATTTATTTCCATGTACGCTGCTTCCTCGTATACC	1	0.14814814814814814	No Hit
ACGTCTGCCTGGGCGTCACGCAGATCGGAAGAGAACACATCTGAACTCCA	1	0.14814814814814814	No Hit
CCGCCGCGTGCCGGCCGGGGGACGGACCGGGAGCAGAGCGGAAGAGCACA	1	0.14814814814814814	No Hit
GAAACTTAAAGGAATTGACGGAAAATCGGAAGAGCACATGTCTGAACTCC	1	0.14814814814814814	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGAACTCGTATG	1	0.14814814814814814	TruSeq Adapter, Index 10 (97% over 36bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATATCGTATG	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TACGTTGTAGTCTAGCTGGTCAGGATATAGAACGTAAGATCACACGTCTT	1	0.14814814814814814	No Hit
TCCAGCCCCGCGTCGCACGGATTCGTAGATCGGAAGGGCACACGTCTGAA	1	0.14814814814814814	Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	66.37037037037037	0.0	0.0	0.0	0.0
2	66.96296296296296	0.0	0.0	0.0	0.0
3	67.11111111111111	0.0	0.0	0.0	0.0
4	67.11111111111111	0.0	0.0	0.0	0.0
5	67.11111111111111	0.0	0.0	0.0	0.0
6	67.11111111111111	0.0	0.0	0.0	0.0
7	70.51851851851852	0.0	0.0	0.0	0.0
8	70.51851851851852	0.0	0.0	0.0	0.0
9	70.66666666666667	0.0	0.0	0.0	0.0
10	70.81481481481481	0.0	0.0	0.0	0.0
11	70.96296296296296	0.0	0.0	0.0	0.0
12	70.96296296296296	0.0	0.0	0.0	0.0
13	71.11111111111111	0.0	0.0	0.0	0.0
14	71.25925925925925	0.0	0.0	0.0	0.0
15	71.25925925925925	0.0	0.0	0.0	0.0
16	71.4074074074074	0.0	0.0	0.0	0.0
17	71.55555555555556	0.0	0.0	0.0	0.0
18	71.55555555555556	0.0	0.0	0.0	0.0
19	71.85185185185185	0.0	0.0	0.0	0.0
20	72.44444444444444	0.0	0.0	0.0	0.0
21	75.4074074074074	0.0	0.0	0.0	0.0
22	76.74074074074075	0.0	0.0	0.0	0.0
23	77.48148148148148	0.0	0.0	0.0	0.0
24	77.92592592592592	0.0	0.0	0.0	0.0
25	79.11111111111111	0.0	0.0	0.0	0.0
26	79.70370370370371	0.0	0.0	0.0	0.0
27	80.29629629629629	0.0	0.0	0.0	0.0
28	83.25925925925925	0.0	0.0	0.0	0.0
29	83.4074074074074	0.0	0.0	0.0	0.0
30	83.85185185185185	0.0	0.0	0.0	0.0
31	85.48148148148148	0.0	0.0	0.0	0.0
32	85.62962962962963	0.0	0.0	0.0	0.0
33	85.77777777777777	0.0	0.0	0.0	0.0
34	86.07407407407408	0.0	0.0	0.0	0.0
35	86.37037037037037	0.0	0.0	0.0	0.0
36	86.51851851851852	0.0	0.0	0.0	0.0
37	86.81481481481481	0.0	0.0	0.0	0.0
38	86.81481481481481	0.0	0.0	0.0	0.0
39	86.81481481481481	0.0	0.0	0.0	0.0
40	86.96296296296296	0.0	0.0	0.0	0.0
41	87.11111111111111	0.0	0.0	0.0	0.0
42	87.11111111111111	0.0	0.0	0.0	0.0
43	87.25925925925925	0.0	0.0	0.0	0.0
44	87.25925925925925	0.0	0.0	0.0	0.0
45	87.25925925925925	0.0	0.0	0.0	0.0
46	87.25925925925925	0.0	0.0	0.0	0.0
47	87.25925925925925	0.0	0.0	0.0	0.0
48	87.25925925925925	0.0	0.0	0.0	0.0
49	87.25925925925925	0.0	0.0	0.0	0.0
50	87.25925925925925	0.0	0.0	0.0	0.0
51	87.25925925925925	0.0	0.0	0.0	0.0
52	87.25925925925925	0.0	0.0	0.0	0.0
53	87.25925925925925	0.0	0.0	0.0	0.0
54	87.25925925925925	0.0	0.0	0.0	0.0
55	87.25925925925925	0.0	0.0	0.0	0.0
56	87.25925925925925	0.0	0.0	0.0	0.0
57	87.25925925925925	0.0	0.0	0.0	0.0
58	87.25925925925925	0.0	0.0	0.0	0.0
59	87.25925925925925	0.0	0.0	0.0	0.0
60	87.25925925925925	0.0	0.0	0.0	0.0
61	87.25925925925925	0.0	0.0	0.0	0.0
62	87.25925925925925	0.0	0.0	0.0	0.0
63	87.25925925925925	0.0	0.0	0.0	0.0
64	87.25925925925925	0.0	0.0	0.0	0.0
65	87.25925925925925	0.0	0.0	0.0	0.0
66	87.25925925925925	0.0	0.0	0.0	0.0
67	87.25925925925925	0.0	0.0	0.0	0.0
68	87.25925925925925	0.0	0.0	0.0	0.0
69	87.25925925925925	0.0	0.0	0.0	0.0
70	87.25925925925925	0.0	0.0	0.0	0.0
71	87.25925925925925	0.0	0.0	0.0	0.0
72	87.25925925925925	0.0	0.0	0.0	0.0
73	87.25925925925925	0.0	0.0	0.0	0.0
74	87.25925925925925	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAAGAGC	65	8.367351E-11	49.230766	7
GGAAGAG	65	8.367351E-11	49.230766	6
GATCGGA	60	6.2918843E-9	46.666664	2
TCGGAAG	60	6.2918843E-9	46.666664	4
CGGAAGA	60	6.2918843E-9	46.666664	5
ATCGGAA	60	6.2918843E-9	46.666664	3
AGATCGG	60	6.2918843E-9	46.666664	1
AAGAGCA	70	1.6189006E-10	45.714287	8
AGAGCAC	70	1.6189006E-10	45.714287	9
AGGGGGG	25	4.289079E-5	40.0	74-75
AAGGGGG	25	4.289079E-5	40.0	72-73
AAAAGGG	25	4.289079E-5	40.0	70-71
AAAGGGG	25	4.289079E-5	40.0	72-73
AAAAAGG	25	4.289079E-5	40.0	70-71
AAAAAAG	25	4.289079E-5	40.0	68-69
GAAAAAA	50	3.103014E-8	32.0	64-65
TTGAAAA	50	3.103014E-8	32.0	62-63
CTTGAAA	50	3.103014E-8	32.0	60-61
TGAAAAA	50	3.103014E-8	32.0	62-63
GCTTGAA	55	7.209019E-8	29.090908	60-61
>>END_MODULE
Rejected 33 READS because READLEN < 1
Read 33 spots for SRR11699361.sra
Rejected 33 READS because READLEN < 1
Read 33 spots for SRR11699361.sra
Rejected 33 READS because READLEN < 1
Read 33 spots for SRR11699361.sra
Written 33 spots for SRR11699361.sra
Rejected 33 READS because READLEN < 1
Read 33 spots for SRR11699361.sra
Rejected 33 READS because READLEN < 1
Read 33 spots for SRR11699361.sra
Rejected 33 READS because READLEN < 1
Read 33 spots for SRR11699361.sra
Rejected 33 READS because READLEN < 1
Read 33 spots for SRR11699361.sra
Rejected 33 READS because READLEN < 1
Read 33 spots for SRR11699361.sra
Rejected 33 READS because READLEN < 1
Read 33 spots for SRR11699361.sra
Rejected 33 READS because READLEN < 1
Read 33 spots for SRR11699361.sra
Rejected 33 READS because READLEN < 1
Read 33 spots for SRR11699361.sra
Rejected 33 READS because READLEN < 1
Read 33 spots for SRR11699361.sra
Rejected 33 READS because READLEN < 1
Read 33 spots for SRR11699361.sra
Rejected 33 READS because READLEN < 1
Read 33 spots for SRR11699361.sra
Rejected 33 READS because READLEN < 1
Read 33 spots for SRR11699361.sra
Rejected 33 READS because READLEN < 1
Read 33 spots for SRR11699361.sra
Rejected 33 READS because READLEN < 1
Read 33 spots for SRR11699361.sra
Rejected 33 READS because READLEN < 1
Read 33 spots for SRR11699361.sra
Rejected 48 READS because READLEN < 1
Read 48 spots for SRR11699361.sra
Rejected 33 READS because READLEN < 1
Read 33 spots for SRR11699361.sra
Written 33 spots for SRR11699361.sra
Written 33 spots for SRR11699361.sra
Written 33 spots for SRR11699361.sra
Written 33 spots for SRR11699361.sra
Written 33 spots for SRR11699361.sra
Written 33 spots for SRR11699361.sra
Written 33 spots for SRR11699361.sra
Written 33 spots for SRR11699361.sra
Written 33 spots for SRR11699361.sra
Written 33 spots for SRR11699361.sra
Written 33 spots for SRR11699361.sra
Written 33 spots for SRR11699361.sra
Written 33 spots for SRR11699361.sra
Written 33 spots for SRR11699361.sra
Written 33 spots for SRR11699361.sra
Written 33 spots for SRR11699361.sra
Written 33 spots for SRR11699361.sra
Written 48 spots for SRR11699361.sra
Written 33 spots for SRR11699361.sra
SRR ids: ['SRR11699361.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd__buu2c9x
SRR11699361.sra spots: 675
blocks: [[1, 33], [34, 66], [67, 99], [100, 132], [133, 165], [166, 198], [199, 231], [232, 264], [265, 297], [298, 330], [331, 363], [364, 396], [397, 429], [430, 462], [463, 495], [496, 528], [529, 561], [562, 594], [595, 627], [628, 675]]
SRR11699361 file size 137
SRR11699361 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR11699361 SRR11699361_1.fastq
Input file:	SRR11699361_1.fastq
trimmed:	SRR11699361-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	20
Wed Jun 18 20:38:52 2025 >> started

Wed Jun 18 20:38:52 2025 >> done (0.158s)
675 reads processed; of these:
 40 ( 5.93%) short reads filtered out after trimming by size control
471 (69.78%) empty reads filtered out after trimming by size control
164 (24.30%) reads available; of these:
132 (80.49%) trimmed reads available after processing
 32 (19.51%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  2	  1.22%
 19	  4	  2.44%
 20	 24	 14.63%
 21	 10	  6.10%
 22	 11	  6.71%
 23	  4	  2.44%
 24	 13	  7.93%
 25	  5	  3.05%
 26	  6	  3.66%
 27	 23	 14.02%
 28	  2	  1.22%
 29	  3	  1.83%
 30	 12	  7.32%
 31	  1	  0.61%
 32	  1	  0.61%
 33	  1	  0.61%
 34	  4	  2.44%
 35	  1	  0.61%
 36	  2	  1.22%
 37	  0	  0.00%
 38	  0	  0.00%
 39	  1	  0.61%
 40	  1	  0.61%
 41	  0	  0.00%
 42	  1	  0.61%
 43	  0	  0.00%
 44	  0	  0.00%
 45	  0	  0.00%
 46	  0	  0.00%
 47	  0	  0.00%
 48	  0	  0.00%
 49	  0	  0.00%
 50	  0	  0.00%
 51	  0	  0.00%
 52	  0	  0.00%
 53	  0	  0.00%
 54	  0	  0.00%
 55	  0	  0.00%
 56	  0	  0.00%
 57	  0	  0.00%
 58	  0	  0.00%
 59	  0	  0.00%
 60	  0	  0.00%
 61	  0	  0.00%
 62	  0	  0.00%
 63	  0	  0.00%
 64	  0	  0.00%
 65	  0	  0.00%
 66	  0	  0.00%
 67	  0	  0.00%
 68	  0	  0.00%
 69	  0	  0.00%
 70	  0	  0.00%
 71	  0	  0.00%
 72	  0	  0.00%
 73	  0	  0.00%
 74	  0	  0.00%
 75	  0	  0.00%
 76	  0	  0.00%
 77	  0	  0.00%
 78	  0	  0.00%
 79	  0	  0.00%
 80	  0	  0.00%
 81	  0	  0.00%
 82	  0	  0.00%
 83	  0	  0.00%
 84	  0	  0.00%
 85	  0	  0.00%
 86	 32	 19.51%
164 reads passed initial QC
                                 Started job on |	Jun 18 20:43:18
                             Started mapping on |	Jun 18 20:43:57
                                    Finished on |	Jun 18 20:44:06
       Mapping speed, Million of reads per hour |	0.07

                          Number of input reads |	164
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	21
                        Uniquely mapped reads % |	12.80%
                          Average mapped length |	19.90
                       Number of splices: Total |	0
            Number of splices: Annotated (sjdb) |	0
                       Number of splices: GT/AG |	0
                       Number of splices: GC/AG |	0
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	0
                      Mismatch rate per base, % |	0.72%
                         Deletion rate per base |	0.00%
                        Deletion average length |	0.00
                        Insertion rate per base |	0.00%
                       Insertion average length |	0.00
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	33
             % of reads mapped to multiple loci |	20.12%
        Number of reads mapped to too many loci |	24
             % of reads mapped to too many loci |	14.63%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	20.73%
                     % of reads unmapped: other |	31.71%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	110	110	110
N_multimapping	33	33	33
N_noFeature	16	18	19
N_ambiguous	0	0	0
UnstrandedReadsAssigned:5 PositiveStrandReadsAssigned:3 NegativeStrandReadsAssigned:2
Dataset is classified unstranded
MeadianReadLen=19 20thPercentileLength=13 echo kmer=19
SRR11699361 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 72,539
[index] number of k-mers: 56,563,136
[index] number of equivalence classes: 414,827
[quant] running in single-end mode
[quant] will process file 1: SRR11699361-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 164 reads, 36 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 52 rounds

  72540 SRR11699361.ke.tsv
  44303 SRR11699361.se.tsv
 116843 total
==> SRR11699361.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Zm00001eb096110_T001	2067	1968	0	0
Zm00001eb321680_T001	3417	3318	0	0
Zm00001eb323640_T001	3273	3174	0	0
Zm00001eb314140_T001	893	794	0	0
Zm00001eb080260_T001	3529	3430	0	0
Zm00001eb026490_T001	2433	2334	0	0
Zm00001eb026490_T002	2229	2130	0	0
Zm00001eb026490_T003	2220	2121	0	0
Zm00001eb281360_T001	2412	2313	0	0

==> SRR11699361.se.tsv <==
Zm00001eb015280	0
Zm00001eb000610	0
Zm00001eb033210	0
Zm00001eb044610	0
Zm00001eb014360	0
Zm00001eb051320	0
Zm00001eb021860	0
Zm00001eb011690	0
Zm00001eb016840	0
Zm00001eb052060	0
SRR11699361 completed mapping pipeline successfully
