Starting /dee2/code/volunteer_pipeline.sh SRR11699267
    current disk space = 2472591683584
    free memory = 1578944764 
SRR11699267 SRAfilesize
bcaa36838d81b39e7b4e11e0d23914a0  SRR11699267.sra
SRR11699267.sra file validated
SRR11699267 is single end
SRR11699267 is conventional basespace
SRR11699267 read1 length is 76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR11699267_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3025	32.0	32.0	32.0	32.0	32.0
2	31.42725	32.0	32.0	32.0	32.0	32.0
3	31.47025	32.0	32.0	32.0	32.0	32.0
4	31.534	32.0	32.0	32.0	32.0	32.0
5	31.54625	32.0	32.0	32.0	32.0	32.0
6	35.167	36.0	36.0	36.0	36.0	36.0
7	35.25175	36.0	36.0	36.0	36.0	36.0
8	35.1045	36.0	36.0	36.0	36.0	36.0
9	35.016	36.0	36.0	36.0	36.0	36.0
10-11	35.118750000000006	36.0	36.0	36.0	36.0	36.0
12-13	35.1285	36.0	36.0	36.0	36.0	36.0
14-15	35.11175	36.0	36.0	36.0	36.0	36.0
16-17	35.15025	36.0	36.0	36.0	36.0	36.0
18-19	35.208375000000004	36.0	36.0	36.0	36.0	36.0
20-21	35.068875	36.0	36.0	36.0	36.0	36.0
22-23	35.092375000000004	36.0	36.0	36.0	36.0	36.0
24-25	35.209125	36.0	36.0	36.0	36.0	36.0
26-27	35.08	36.0	36.0	36.0	36.0	36.0
28-29	35.038	36.0	36.0	36.0	36.0	36.0
30-31	34.995125	36.0	36.0	36.0	36.0	36.0
32-33	34.94225	36.0	36.0	36.0	36.0	36.0
34-35	35.037125	36.0	36.0	36.0	36.0	36.0
36-37	35.00975	36.0	36.0	36.0	36.0	36.0
38-39	34.95375	36.0	36.0	36.0	36.0	36.0
40-41	34.932375	36.0	36.0	36.0	36.0	36.0
42-43	34.943375	36.0	36.0	36.0	36.0	36.0
44-45	34.93575	36.0	36.0	36.0	36.0	36.0
46-47	34.974875	36.0	36.0	36.0	36.0	36.0
48-49	34.7675	36.0	36.0	36.0	34.0	36.0
50-51	34.628125	36.0	36.0	36.0	32.0	36.0
52-53	34.575374999999994	36.0	36.0	36.0	32.0	36.0
54-55	34.287375	36.0	36.0	36.0	32.0	36.0
56-57	34.042625	36.0	36.0	36.0	32.0	36.0
58-59	33.936125000000004	36.0	36.0	36.0	32.0	36.0
60-61	33.98825	36.0	36.0	36.0	32.0	36.0
62-63	33.861000000000004	36.0	36.0	36.0	32.0	36.0
64-65	33.773624999999996	36.0	36.0	36.0	32.0	36.0
66-67	33.11525	36.0	32.0	36.0	27.0	36.0
68-69	33.191625	36.0	32.0	36.0	27.0	36.0
70-71	33.431749999999994	36.0	34.0	36.0	27.0	36.0
72-73	33.193875	36.0	34.0	36.0	24.0	36.0
74-75	33.608125	36.0	36.0	36.0	29.5	36.0
76	31.33325	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	0.0
21	0.0
22	2.0
23	2.0
24	3.0
25	17.0
26	26.0
27	32.0
28	52.0
29	72.0
30	74.0
31	140.0
32	162.0
33	331.0
34	890.0
35	2196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.400000000000002	31.55	21.525	23.525
2	21.625	28.249999999999996	26.05	24.075
3	25.35	32.625	17.05	24.975
4	27.55	32.65	17.849999999999998	21.95
5	21.349999999999998	28.1	23.35	27.200000000000003
6	26.75	26.674999999999997	24.65	21.925
7	30.675	34.8	18.525	16.0
8	21.8	26.900000000000002	27.900000000000002	23.400000000000002
9	26.474999999999998	30.25	19.55	23.724999999999998
10-11	31.0	28.3125	22.8375	17.849999999999998
12-13	22.5625	23.3	23.8625	30.275000000000002
14-15	29.8375	36.875	17.1875	16.1
16-17	22.75	24.6	31.574999999999996	21.075
18-19	34.9	24.875	23.3125	16.9125
20-21	24.5375	34.75	24.0375	16.675
22-23	30.841355169396174	38.05475684460558	14.701837729716214	16.402050256282035
24-25	29.15	39.7	14.524999999999999	16.625
26-27	29.425	32.337500000000006	17.549999999999997	20.6875
28-29	32.675	28.999999999999996	15.712499999999999	22.6125
30-31	32.6625	36.875	9.7125	20.75
32-33	29.075	39.375	10.95	20.599999999999998
34-35	31.112499999999997	30.325000000000003	13.2875	25.275
36-37	26.337500000000002	33.25	11.4	29.012500000000003
38-39	21.337500000000002	34.6625	13.350000000000001	30.65
40-41	23.7625	29.025000000000002	17.6625	29.549999999999997
42-43	24.975	24.7375	21.9375	28.349999999999998
44-45	20.3625	31.775	18.05	29.812499999999996
46-47	19.75	32.3125	16.662499999999998	31.275
48-49	13.375	29.95	20.45	36.225
50-51	11.662500000000001	31.0125	21.8	35.525
52-53	15.214401800225028	30.30378797349669	22.95286910863858	31.528941117639704
54-55	12.9	31.887500000000003	24.637500000000003	30.575000000000003
56-57	8.025	31.075000000000003	27.5875	33.3125
58-59	7.9125	37.075	26.1125	28.9
60-61	8.0625	36.5	29.512500000000003	25.924999999999997
62-63	9.7375	31.837500000000002	28.875	29.549999999999997
64-65	10.2	25.7	29.9875	34.112500000000004
66-67	13.425	20.7	33.4375	32.4375
68-69	15.037500000000001	18.9625	36.1125	29.8875
70-71	16.802100262532814	20.302537817227154	36.96712089011127	25.928241030128767
72-73	17.4875	18.1625	33.9625	30.3875
74-75	20.8875	13.025	32.35	33.7375
76	15.125	11.75	40.6	32.525
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.5
29	1.0
30	0.5
31	0.5
32	0.5
33	0.0
34	1.5
35	2.0
36	3.0
37	5.0
38	5.5
39	13.5
40	36.5
41	66.0
42	80.0
43	135.5
44	300.5
45	718.0
46	1026.0
47	774.0
48	529.5
49	474.5
50	412.0
51	364.5
52	241.0
53	133.0
54	101.0
55	96.5
56	63.0
57	21.0
58	8.0
59	5.5
60	3.0
61	2.0
62	1.0
63	2.0
64	2.0
65	0.5
66	0.5
67	1.0
68	1.0
69	0.5
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0125
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0125
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0125
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.07297830374753	56.45
2	5.128205128205128	6.5
3	2.130177514792899	4.05
4	0.8284023668639053	2.1
5	0.5522682445759369	1.7500000000000002
6	0.39447731755424065	1.5
7	0.39447731755424065	1.7500000000000002
8	0.1183431952662722	0.6
9	0.1183431952662722	0.675
>10	1.143984220907298	15.275
>50	0.07889546351084813	3.4000000000000004
>100	0.03944773175542406	5.949999999999999
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCC	238	5.949999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAAC	76	1.9	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	60	1.5	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACT	43	1.075	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCA	34	0.8500000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCGTTGTAGTCTAGCTGGTCAGGATACTCAGATCGGAAGAGCACACGTC	34	0.8500000000000001	No Hit
TAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCA	33	0.8250000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAG	32	0.8	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGGGCTATAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAA	30	0.75	TruSeq Adapter, Index 3 (97% over 36bp)
ACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGT	30	0.75	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTGAGATCGGAAGAGCAC	30	0.75	No Hit
AACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAT	28	0.7000000000000001	TruSeq Adapter, Index 3 (97% over 36bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	27	0.675	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAA	25	0.625	TruSeq Adapter, Index 3 (97% over 36bp)
TAATTCATGATCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	24	0.6	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCCTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACT	24	0.6	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT	20	0.5	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGATGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA	19	0.475	TruSeq Adapter, Index 7 (97% over 35bp)
AACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	17	0.42500000000000004	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATACAT	16	0.4	TruSeq Adapter, Index 3 (97% over 36bp)
GAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	15	0.375	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATACA	15	0.375	TruSeq Adapter, Index 3 (97% over 36bp)
GCGACCCCAGGTCAGTCGGGACTAGATCGGAAGAGCACACGTCTGAACTC	14	0.35000000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT	14	0.35000000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	13	0.325	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGACACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACT	12	0.3	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACACGACTCTCGGCAACGGATATCTCGGCAGATCGGAAGAGCACACGTC	11	0.27499999999999997	No Hit
GCTGACGCGGGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA	11	0.27499999999999997	TruSeq Adapter, Index 7 (97% over 35bp)
CGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA	10	0.25	TruSeq Adapter, Index 7 (97% over 35bp)
CACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACTCCA	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCTAGATCGGAAGAGCA	10	0.25	No Hit
CTAACGAACGAACGATTTGAATAGATCGGAAGAGCACACGTCTGAACTCC	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACACGACTCTCGGCAACGAGATCGGAAGAGCACACGTCTGAACTCCAG	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGACGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA	9	0.22499999999999998	TruSeq Adapter, Index 7 (97% over 35bp)
GAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTC	9	0.22499999999999998	No Hit
TGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGT	8	0.2	No Hit
GACACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACTC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATAC	8	0.2	TruSeq Adapter, Index 3 (97% over 36bp)
CAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCAGTGATGTATGCAGCTGAGGCATCCTAACGAACGAACGATTTGAACAG	7	0.17500000000000002	No Hit
ACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATA	7	0.17500000000000002	TruSeq Adapter, Index 3 (97% over 36bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTGAGATCGGAAGAGCAC	7	0.17500000000000002	No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCAGATCGGAAGAGCACAC	7	0.17500000000000002	No Hit
TCCTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACGACTCTCGGCAACGGATATCTCGGCAGATCGGAAGAGCACACGTCTG	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAA	7	0.17500000000000002	TruSeq Adapter, Index 3 (97% over 36bp)
GCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACAC	6	0.15	No Hit
ATCCTAACGAACGAACGATTTGAATAGATCGGAAGAGCACACGTCTGAAC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGCCCCGCGTCGCACGGATTCGTAGATCGGAAGAGCACACGTCTGAACT	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGAGCGTAGTTCAGTGGTAAAACATCTCCTTGCCAAGGAGAGATCGGAA	6	0.15	No Hit
TTTGGATTGAAGGGAGCTCTGAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGCATCCTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTC	6	0.15	No Hit
CCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCAGGATAGCTCAGTTGGTAGAGCAGAGGACTGAGATCGGAAGAGCACA	6	0.15	No Hit
GACACGACTCTCGGCAACGGATATCTCGAGATCGGAAGAGCACACGTCTG	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGACCCCAGGTCAGTCGGGAGATCGGAAGAGCACACGTCTGAACTCCAG	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGTAGTTCGACCGTGGATTAGATCGGAAGAGCACACGTCTGAACTCCAGT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCAGATCGGAAGAGCACACG	5	0.125	No Hit
TATGCAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGA	5	0.125	No Hit
GCGGCAAAATAGCTCGATGCCAGAATAAGATCGGAAGAGCACACGTCTGA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGACCCCAGGTCAGTCGGGACTACCAGATCGGAAGAGCACACGTCTGAA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAGGCTGACGCGGGCTCTAGATCGGAAGAGCACACGTCTGAACTCCAGT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATACATCTC	5	0.125	TruSeq Adapter, Index 3 (97% over 36bp)
CGACACGACTCTCGGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCATGTTGCAGCTTCACTAGATCGGAAGAGCACACGTCTGAACTCCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCAC	5	0.125	No Hit
GCGACCCCAGGTCAGTCGGGACTACCCGCTAGATCGGAAGAGCACACGTC	5	0.125	No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACTCAGATCGGAAGAGCACACGTC	5	0.125	No Hit
TACCTGGTTGATCCTGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.025	0.0	0.0	0.0	0.0
2	0.075	0.0	0.0	0.0	0.0
3	0.1	0.0	0.0	0.0	0.0
4	0.15	0.0	0.0	0.0	0.0
5	0.2	0.0	0.0	0.0	0.0
6	0.35	0.0	0.0	0.0	0.0
7	0.425	0.0	0.0	0.0	0.0
8	0.525	0.0	0.0	0.0	0.0
9	1.075	0.0	0.0	0.0	0.0
10	1.625	0.0	0.0	0.0	0.0
11	2.125	0.0	0.0	0.0	0.0
12	2.975	0.0	0.0	0.0	0.0
13	4.425	0.0	0.0	0.0	0.0
14	7.05	0.0	0.0	0.0	0.0
15	9.5	0.0	0.0	0.0	0.0
16	11.425	0.0	0.0	0.0	0.0
17	14.75	0.0	0.0	0.0	0.0
18	18.6	0.0	0.0	0.0	0.0
19	20.925	0.0	0.0	0.0	0.0
20	24.75	0.0	0.0	0.0	0.0
21	28.3	0.0	0.0	0.0	0.0
22	33.65	0.0	0.0	0.0	0.0
23	44.325	0.0	0.0	0.0	0.0
24	47.9	0.0	0.0	0.0	0.0
25	56.825	0.0	0.0	0.0	0.0
26	61.65	0.0	0.0	0.0	0.0
27	64.25	0.0	0.0	0.0	0.0
28	67.425	0.0	0.0	0.0	0.0
29	69.6	0.0	0.0	0.0	0.0
30	71.525	0.0	0.0	0.0	0.0
31	75.35	0.0	0.0	0.0	0.0
32	77.275	0.0	0.0	0.0	0.0
33	79.05	0.0	0.0	0.0	0.0
34	81.25	0.0	0.0	0.0	0.0
35	83.775	0.0	0.0	0.0	0.0
36	86.575	0.0	0.0	0.0	0.0
37	88.85	0.0	0.0	0.0	0.0
38	90.05	0.0	0.0	0.0	0.0
39	90.975	0.0	0.0	0.0	0.0
40	91.7	0.0	0.0	0.0	0.0
41	92.475	0.0	0.0	0.0	0.0
42	93.325	0.0	0.0	0.0	0.0
43	93.85	0.0	0.0	0.0	0.0
44	94.25	0.0	0.0	0.0	0.0
45	94.425	0.0	0.0	0.0	0.0
46	94.8	0.0	0.0	0.0	0.0
47	94.9	0.0	0.0	0.0	0.0
48	94.975	0.0	0.0	0.0	0.0
49	95.2	0.0	0.0	0.0	0.0
50	95.225	0.0	0.0	0.0	0.0
51	95.25	0.0	0.0	0.0	0.0
52	95.275	0.0	0.0	0.0	0.0
53	95.3	0.0	0.0	0.0	0.0
54	95.3	0.0	0.0	0.0	0.0
55	95.3	0.0	0.0	0.0	0.0
56	95.375	0.0	0.0	0.0	0.0
57	95.375	0.0	0.0	0.0	0.0
58	95.375	0.0	0.0	0.0	0.0
59	95.4	0.0	0.0	0.0	0.0
60	95.4	0.0	0.0	0.0	0.0
61	95.4	0.0	0.0	0.0	0.0
62	95.4	0.0	0.0	0.0	0.0
63	95.4	0.0	0.0	0.0	0.0
64	95.4	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACACG	15	0.0020658788	70.00001	1
TCTAGCT	15	0.0020658788	70.00001	11
TCAGGAT	15	0.0020658788	70.00001	20
GCTGGTC	15	0.0020658788	70.00001	15
TAGCTGG	15	0.0020658788	70.00001	13
GGTCAGG	15	0.0020658788	70.00001	18
CTGGTCA	15	0.0020658788	70.00001	16
TGTAGTC	15	0.0020658788	70.00001	6
CTAGCTG	15	0.0020658788	70.00001	12
GTCTAGC	15	0.0020658788	70.00001	10
CAGGATA	15	0.0020658788	70.00001	21
GTCAGGA	15	0.0020658788	70.00001	19
AGCTGGT	15	0.0020658788	70.00001	14
TGGTCAG	20	7.003785E-5	70.0	17
TTGTAGT	25	2.3570374E-6	70.0	5
CCGTTGT	20	7.003785E-5	70.0	2
TAGTCTA	20	7.003785E-5	70.0	8
TCCGTTG	20	7.003785E-5	70.0	1
CGTTGTA	20	7.003785E-5	70.0	3
AGTCTAG	20	7.003785E-5	70.0	9
>>END_MODULE
Rejected 248108 READS because READLEN < 1
Read 248108 spots for SRR11699267.sra
Written 248108 spots for SRR11699267.sra
Rejected 248108 READS because READLEN < 1
Read 248108 spots for SRR11699267.sra
Written 248108 spots for SRR11699267.sra
Rejected 248108 READS because READLEN < 1
Read 248108 spots for SRR11699267.sra
Written 248108 spots for SRR11699267.sra
Rejected 248108 READS because READLEN < 1
Read 248108 spots for SRR11699267.sra
Written 248108 spots for SRR11699267.sra
Rejected 248108 READS because READLEN < 1
Read 248108 spots for SRR11699267.sra
Written 248108 spots for SRR11699267.sra
Rejected 248108 READS because READLEN < 1
Read 248108 spots for SRR11699267.sra
Written 248108 spots for SRR11699267.sra
Rejected 248108 READS because READLEN < 1
Read 248108 spots for SRR11699267.sra
Written 248108 spots for SRR11699267.sra
Rejected 248108 READS because READLEN < 1
Read 248108 spots for SRR11699267.sra
Written 248108 spots for SRR11699267.sra
Rejected 248108 READS because READLEN < 1
Read 248108 spots for SRR11699267.sra
Written 248108 spots for SRR11699267.sra
Rejected 248108 READS because READLEN < 1
Read 248108 spots for SRR11699267.sra
Written 248108 spots for SRR11699267.sra
Rejected 248108 READS because READLEN < 1
Read 248108 spots for SRR11699267.sra
Written 248108 spots for SRR11699267.sra
Rejected 248108 READS because READLEN < 1
Read 248108 spots for SRR11699267.sra
Written 248108 spots for SRR11699267.sra
Rejected 248108 READS because READLEN < 1
Read 248108 spots for SRR11699267.sra
Written 248108 spots for SRR11699267.sra
Rejected 248108 READS because READLEN < 1
Read 248108 spots for SRR11699267.sra
Written 248108 spots for SRR11699267.sra
Rejected 248108 READS because READLEN < 1
Read 248108 spots for SRR11699267.sra
Written 248108 spots for SRR11699267.sra
Rejected 248108 READS because READLEN < 1
Read 248108 spots for SRR11699267.sra
Written 248108 spots for SRR11699267.sra
Rejected 248108 READS because READLEN < 1
Read 248108 spots for SRR11699267.sra
Written 248108 spots for SRR11699267.sra
Rejected 248108 READS because READLEN < 1
Read 248108 spots for SRR11699267.sra
Written 248108 spots for SRR11699267.sra
Rejected 248125 READS because READLEN < 1
Read 248125 spots for SRR11699267.sra
Written 248125 spots for SRR11699267.sra
Rejected 248108 READS because READLEN < 1
Read 248108 spots for SRR11699267.sra
Written 248108 spots for SRR11699267.sra
SRR ids: ['SRR11699267.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_ufam1187
SRR11699267.sra spots: 4962177
blocks: [[1, 248108], [248109, 496216], [496217, 744324], [744325, 992432], [992433, 1240540], [1240541, 1488648], [1488649, 1736756], [1736757, 1984864], [1984865, 2232972], [2232973, 2481080], [2481081, 2729188], [2729189, 2977296], [2977297, 3225404], [3225405, 3473512], [3473513, 3721620], [3721621, 3969728], [3969729, 4217836], [4217837, 4465944], [4465945, 4714052], [4714053, 4962177]]
SRR11699267 file size 942776
SRR11699267 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR11699267 SRR11699267_1.fastq
Input file:	SRR11699267_1.fastq
trimmed:	SRR11699267-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	20
Wed Jun 18 05:43:48 2025 >> started

Wed Jun 18 05:43:50 2025 >> done (2.144s)
4962177 reads processed; of these:
1046726 (21.09%) short reads filtered out after trimming by size control
   2811 ( 0.06%) empty reads filtered out after trimming by size control
3912640 (78.85%) reads available; of these:
3847575 (98.34%) trimmed reads available after processing
  65065 ( 1.66%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	 131111	  3.35%
 19	 212838	  5.44%
 20	 195321	  4.99%
 21	 308583	  7.89%
 22	 513623	 13.13%
 23	 190300	  4.86%
 24	 448172	 11.45%
 25	 239132	  6.11%
 26	 132712	  3.39%
 27	 147269	  3.76%
 28	 107635	  2.75%
 29	 110113	  2.81%
 30	 198453	  5.07%
 31	 108318	  2.77%
 32	  90458	  2.31%
 33	 103948	  2.66%
 34	 103179	  2.64%
 35	 136295	  3.48%
 36	 105545	  2.70%
 37	  61185	  1.56%
 38	  42878	  1.10%
 39	  41884	  1.07%
 40	  29953	  0.77%
 41	  35778	  0.91%
 42	  22455	  0.57%
 43	  18707	  0.48%
 44	  11728	  0.30%
 45	      0	  0.00%
 46	      0	  0.00%
 47	      0	  0.00%
 48	      0	  0.00%
 49	      0	  0.00%
 50	      0	  0.00%
 51	      0	  0.00%
 52	      0	  0.00%
 53	      0	  0.00%
 54	      0	  0.00%
 55	      0	  0.00%
 56	      0	  0.00%
 57	      0	  0.00%
 58	      0	  0.00%
 59	      0	  0.00%
 60	      0	  0.00%
 61	      0	  0.00%
 62	      0	  0.00%
 63	      0	  0.00%
 64	      0	  0.00%
 65	      0	  0.00%
 66	      0	  0.00%
 67	      0	  0.00%
 68	      0	  0.00%
 69	      0	  0.00%
 70	      0	  0.00%
 71	      0	  0.00%
 72	      1	  0.00%
 73	      0	  0.00%
 74	      0	  0.00%
 75	      1	  0.00%
 76	  65065	  1.66%
3912640 reads passed initial QC


criterion=sequence-density
sequence-density=3.99
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=12
prefix-density=0.01
prefix-fanout=1.0
sequence=TCCGTTGTAGTCTAGCTGGTCAGGATA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=32
fanout-score=64.71
fanout-score-rank=1
prefix-density=0.40
prefix-fanout=1.0
sequence=CCTCGTGTTTAGTTGCCACTATGAGTTTGGAACCCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGA
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	Jun 18 05:44:08
                             Started mapping on |	Jun 18 05:44:09
                                    Finished on |	Jun 18 05:44:22
       Mapping speed, Million of reads per hour |	1083.50

                          Number of input reads |	3912640
                      Average input read length |	27
                                    UNIQUE READS:
                   Uniquely mapped reads number |	377058
                        Uniquely mapped reads % |	9.64%
                          Average mapped length |	23.89
                       Number of splices: Total |	9041
            Number of splices: Annotated (sjdb) |	4413
                       Number of splices: GT/AG |	8406
                       Number of splices: GC/AG |	473
                       Number of splices: AT/AC |	6
               Number of splices: Non-canonical |	156
                      Mismatch rate per base, % |	1.12%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.49
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.35
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	481777
             % of reads mapped to multiple loci |	12.31%
        Number of reads mapped to too many loci |	786720
             % of reads mapped to too many loci |	20.11%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	5.58%
                     % of reads unmapped: other |	52.36%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	3053805	3053805	3053805
N_multimapping	481777	481777	481777
N_noFeature	300325	310903	363505
N_ambiguous	3029	35	28
UnstrandedReadsAssigned:73704 PositiveStrandReadsAssigned:66120 NegativeStrandReadsAssigned:13525
Dataset is classified unstranded
MeadianReadLen=24 20thPercentileLength=21 echo kmer=19
SRR11699267 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 72,539
[index] number of k-mers: 56,563,136
[index] number of equivalence classes: 414,827
[quant] running in single-end mode
[quant] will process file 1: SRR11699267-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 3,912,640 reads, 793,878 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 937 rounds

  72540 SRR11699267.ke.tsv
  44303 SRR11699267.se.tsv
 116843 total
==> SRR11699267.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Zm00001eb096110_T001	2067	1968	0	0
Zm00001eb321680_T001	3417	3318	4	1.64731
Zm00001eb323640_T001	3273	3174	0	0
Zm00001eb314140_T001	893	794	0	0
Zm00001eb080260_T001	3529	3430	0	0
Zm00001eb026490_T001	2433	2334	0	0
Zm00001eb026490_T002	2229	2130	0	0
Zm00001eb026490_T003	2220	2121	0	0
Zm00001eb281360_T001	2412	2313	1	0.590766

==> SRR11699267.se.tsv <==
Zm00001eb015280	0
Zm00001eb000610	0
Zm00001eb033210	0
Zm00001eb044610	0
Zm00001eb014360	0
Zm00001eb051320	0
Zm00001eb021860	0
Zm00001eb011690	0
Zm00001eb016840	0
Zm00001eb052060	0
SRR11699267 completed mapping pipeline successfully
