Starting /dee2/code/volunteer_pipeline.sh SRR11699293
    current disk space = 2469244686336
    free memory = 437460008 
SRR11699293 SRAfilesize
339294ec2121927e522e01dc2ce6b977  SRR11699293.sra
SRR11699293.sra file validated
SRR11699293 is single end
SRR11699293 is conventional basespace
SRR11699293 read1 length is 76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR11699293_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3305	32.0	32.0	32.0	32.0	32.0
2	31.5175	32.0	32.0	32.0	32.0	32.0
3	31.5295	32.0	32.0	32.0	32.0	32.0
4	31.55175	32.0	32.0	32.0	32.0	32.0
5	31.60075	32.0	32.0	32.0	32.0	32.0
6	35.08475	36.0	36.0	36.0	36.0	36.0
7	35.14625	36.0	36.0	36.0	36.0	36.0
8	35.3405	36.0	36.0	36.0	36.0	36.0
9	35.1055	36.0	36.0	36.0	36.0	36.0
10-11	35.252375	36.0	36.0	36.0	36.0	36.0
12-13	35.23325	36.0	36.0	36.0	36.0	36.0
14-15	35.19525	36.0	36.0	36.0	36.0	36.0
16-17	35.2025	36.0	36.0	36.0	36.0	36.0
18-19	35.268625	36.0	36.0	36.0	36.0	36.0
20-21	35.152125	36.0	36.0	36.0	36.0	36.0
22-23	35.197874999999996	36.0	36.0	36.0	36.0	36.0
24-25	35.176875	36.0	36.0	36.0	36.0	36.0
26-27	35.01175	36.0	36.0	36.0	36.0	36.0
28-29	35.054125	36.0	36.0	36.0	36.0	36.0
30-31	35.0265	36.0	36.0	36.0	36.0	36.0
32-33	35.048	36.0	36.0	36.0	36.0	36.0
34-35	35.016375	36.0	36.0	36.0	36.0	36.0
36-37	35.11125	36.0	36.0	36.0	36.0	36.0
38-39	35.056375	36.0	36.0	36.0	36.0	36.0
40-41	35.011250000000004	36.0	36.0	36.0	36.0	36.0
42-43	34.986000000000004	36.0	36.0	36.0	36.0	36.0
44-45	34.949875	36.0	36.0	36.0	36.0	36.0
46-47	35.0185	36.0	36.0	36.0	36.0	36.0
48-49	34.887249999999995	36.0	36.0	36.0	36.0	36.0
50-51	34.64425	36.0	36.0	36.0	32.0	36.0
52-53	34.51875	36.0	36.0	36.0	32.0	36.0
54-55	34.205625	36.0	36.0	36.0	32.0	36.0
56-57	33.503	36.0	36.0	36.0	27.0	36.0
58-59	33.257375	36.0	36.0	36.0	27.0	36.0
60-61	33.0745	36.0	34.0	36.0	20.5	36.0
62-63	32.692125	36.0	34.0	36.0	14.0	36.0
64-65	32.542125	36.0	32.0	36.0	14.0	36.0
66-67	31.858875	36.0	32.0	36.0	14.0	36.0
68-69	31.933875	36.0	32.0	36.0	14.0	36.0
70-71	32.081875	36.0	32.0	36.0	14.0	36.0
72-73	32.245875	36.0	32.0	36.0	14.0	36.0
74-75	32.608125	36.0	32.0	36.0	14.0	36.0
76	28.1445	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	0.0
22	3.0
23	7.0
24	8.0
25	22.0
26	27.0
27	45.0
28	54.0
29	79.0
30	102.0
31	154.0
32	249.0
33	409.0
34	1060.0
35	1778.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.875	28.999999999999996	24.375	21.75
2	26.35	24.725	23.200000000000003	25.724999999999998
3	26.224999999999998	30.9	16.400000000000002	26.474999999999998
4	31.624999999999996	31.25	17.625	19.5
5	20.0	25.3	26.75	27.950000000000003
6	24.675	24.875	30.875000000000004	19.575
7	34.675	34.675	16.875	13.775
8	20.925	24.725	35.5	18.85
9	24.425	37.55	16.2	21.825
10-11	33.037499999999994	26.9125	23.474999999999998	16.575
12-13	19.475	21.55	27.1375	31.837500000000002
14-15	30.1875	39.4125	16.8125	13.5875
16-17	21.825	22.8	35.625	19.75
18-19	40.112500000000004	23.3625	21.512500000000003	15.012500000000001
20-21	22.162499999999998	32.7	27.150000000000002	17.9875
22-23	34.5375	38.5625	12.712499999999999	14.1875
24-25	29.862499999999997	40.637499999999996	13.725000000000001	15.775
26-27	26.575	34.050000000000004	18.35	21.025
28-29	29.875	27.9125	16.5625	25.650000000000002
30-31	33.1	34.5	10.1875	22.2125
32-33	27.625	36.3625	12.375	23.6375
34-35	30.3	29.9375	15.75	24.0125
36-37	27.1	35.05	11.725	26.125
38-39	23.65	34.0	14.0625	28.287499999999998
40-41	24.525	29.6375	15.8875	29.95
42-43	28.050000000000004	27.375	18.587500000000002	25.9875
44-45	20.5375	34.150000000000006	15.325	29.9875
46-47	20.6125	32.0	14.5625	32.824999999999996
48-49	15.862499999999999	29.849999999999998	16.275000000000002	38.012499999999996
50-51	15.312500000000002	31.6875	16.5	36.5
52-53	18.025	27.875	17.9875	36.1125
54-55	15.6125	30.7	18.8375	34.849999999999994
56-57	13.200000000000001	33.6125	17.5	35.6875
58-59	16.7875	32.875	14.762500000000001	35.575
60-61	12.925	29.825000000000003	19.7125	37.5375
62-63	13.9875	33.1875	19.75	33.074999999999996
64-65	12.587499999999999	30.8	22.3625	34.25
66-67	17.3875	19.650000000000002	27.5875	35.375
68-69	14.924999999999999	23.4875	27.5875	34.0
70-71	17.925	23.575	29.4125	29.0875
72-73	20.125	18.575	28.237499999999997	33.0625
74-75	18.75	17.0625	30.6375	33.550000000000004
76	14.649999999999999	15.675	39.324999999999996	30.349999999999998
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.5
27	0.5
28	0.0
29	0.0
30	1.0
31	1.5
32	1.0
33	1.0
34	0.5
35	0.0
36	0.5
37	1.0
38	2.0
39	3.0
40	6.5
41	17.0
42	24.0
43	39.0
44	69.5
45	137.5
46	190.0
47	318.5
48	708.5
49	794.5
50	619.0
51	609.5
52	521.0
53	351.5
54	261.0
55	194.0
56	99.0
57	62.5
58	54.0
59	37.0
60	12.5
61	4.5
62	4.0
63	3.0
64	1.5
65	0.5
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.06933219906423	51.175000000000004
2	6.252658443215653	7.35
3	2.1692896639727772	3.8249999999999997
4	1.105912377711612	2.6
5	0.680561463207146	2.0
6	0.340280731603573	1.2
7	0.1701403658017865	0.7000000000000001
8	0.46788600595491275	2.1999999999999997
9	0.2977456401531263	1.575
>10	1.1484474691620588	13.675
>50	0.25521054870267973	9.125
>100	0.04253509145044662	4.575
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCC	183	4.575	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCA	75	1.875	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAAC	65	1.625	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGCTATAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG	63	1.575	TruSeq Adapter, Index 15 (97% over 36bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTGAGATCGGAAGAGCAC	56	1.4000000000000001	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	54	1.35	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTTGTAGTCTAGCTGGTCAGGATACTCAGATCGGAAGAGCACACGTC	52	1.3	No Hit
GAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	40	1.0	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCCTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACT	34	0.8500000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACT	31	0.775	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG	28	0.7000000000000001	TruSeq Adapter, Index 15 (97% over 36bp)
CCAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT	28	0.7000000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGT	28	0.7000000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAG	26	0.65	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGA	25	0.625	TruSeq Adapter, Index 15 (97% over 36bp)
TAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCA	24	0.6	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCAGATCGGAAGAGCACAC	24	0.6	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCTAGATCGGAAGAGCA	23	0.575	No Hit
ATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGACAAT	23	0.575	TruSeq Adapter, Index 15 (97% over 36bp)
TCCGTTGTAGTCTAGTTGGTCAGGATACTCAGATCGGAAGAGCACACGTC	22	0.5499999999999999	No Hit
GAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	21	0.525	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAATTCATGATCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	21	0.525	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC	16	0.4	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	16	0.4	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCGTTGTAGTCTAGGTGGTTAGGATACTCAGATCGGAAGAGCACACGTC	15	0.375	No Hit
GATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGACAA	14	0.35000000000000003	TruSeq Adapter, Index 15 (97% over 36bp)
TAATTCATGATCTGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA	14	0.35000000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	12	0.3	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCGTTGTAGTCTAGTTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTC	11	0.27499999999999997	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCAGATCGGAAGAGCACACG	10	0.25	No Hit
CGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTC	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTAGATCGGAAGAGCACA	10	0.25	No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTGTAGATCGGAAGAGCA	10	0.25	No Hit
CGACACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACT	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC	9	0.22499999999999998	TruSeq Adapter, Index 5 (100% over 35bp)
TGCAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGC	9	0.22499999999999998	No Hit
AGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGAC	9	0.22499999999999998	TruSeq Adapter, Index 15 (97% over 36bp)
GAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTC	9	0.22499999999999998	No Hit
ACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATTCTGGTGTCCTAGGCGTAGAGGAACCACAAGATCGGAAGAGCACACG	9	0.22499999999999998	No Hit
AACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGACACGACTCTCGGCAACGAGATCGGAAGAGCACACGTCTGAACTCCAG	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCCAGATCGGAAGAGCACA	8	0.2	No Hit
TAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGT	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTAACGAACGAACGATTTGAATAGATCGGAAGAGCACACGTCTGAACTCC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTGAGATCGGAAGAGCAC	8	0.2	No Hit
GGAGAGATGGCTGAGTGGACTAAAGCGGCGGATTAGATCGGAAGAGCACA	8	0.2	No Hit
TCCTCAGTAGCTCAGTGGTAGAGCGGTCGGCTGAGATCGGAAGAGCACAC	8	0.2	No Hit
AGGGATGTAGCGCAGCTTGGTAGCGCTTTTGTTTTAGATCGGAAGAGCAC	8	0.2	No Hit
TGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGT	7	0.17500000000000002	No Hit
GGAGAGATGGCTGAGTGGACTAAAGCGGCGGATTGAGATCGGAAGAGCAC	7	0.17500000000000002	No Hit
CATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAA	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCTAGATCGGAAGAGCACAC	7	0.17500000000000002	No Hit
GGGTCGATGCCCGAGCGGTTAATGGGGACGGACTGTAGATCGGAAGAGCA	6	0.15	No Hit
TCAAAAGAGGAAAGGCTTGCGGTGGATACCTAGGCACCCAGAGATCGGAA	6	0.15	No Hit
GCGGCAAAATAGCTCGATGCCAGAATAGATCGGAAGAGCACACGTCTGAA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG	6	0.15	TruSeq Adapter, Index 15 (97% over 36bp)
AAAATAGCTCGATGCCAGAATAAGATCGGAAGAGCACACGTCTGAACTCC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCCAGATCGGAAGAGCACACGTCT	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCAGGATAGCTCAGTTGGTAGAGCAGAGGACTGAGATCGGAAGAGCACA	6	0.15	No Hit
CGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGACA	6	0.15	TruSeq Adapter, Index 15 (97% over 36bp)
AGGCATCCTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTG	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATTCTGGTGTCCTAGGCGTAGAGGAACCACACCAGATCGGAAGAGCACA	5	0.125	No Hit
TATTCTGGTGTCCTAGGCGTAGAGGAACCACACCAATCAGATCGGAAGAG	5	0.125	No Hit
CGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GACACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACTC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACA	5	0.125	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCCAGATCGGAAGAGCA	5	0.125	No Hit
GGGCCTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACG	5	0.125	TruSeq Adapter, Index 15 (97% over 36bp)
GCGAGCGTAGTTCAGTGGTAAAACATCTCCTTGCCAAGGAGAGATCGGAA	5	0.125	No Hit
AGCGGAGTAGAGCAGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTAGATCGGAAGAGCACA	5	0.125	No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTGCTCTTAGATCGGAAGAGCAC	5	0.125	No Hit
GCGACCCCAGGTCAGTCGGGACTAGATCGGAAGAGCACACGTCTGAACTC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTAGATCGGAAGAGCACA	5	0.125	No Hit
CGACACGACTCTCGGCAACGGAAGATCGGAAGAGCACACGTCTGAACTCC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAGATCGGAAGAGCACACGTCTGAA	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.075	0.0	0.0	0.0	0.0
2	0.125	0.0	0.0	0.0	0.0
3	0.2	0.0	0.0	0.0	0.0
4	0.225	0.0	0.0	0.0	0.0
5	0.3	0.0	0.0	0.0	0.0
6	0.4	0.0	0.0	0.0	0.0
7	0.5	0.0	0.0	0.0	0.0
8	0.6	0.0	0.0	0.0	0.0
9	1.3	0.0	0.0	0.0	0.0
10	1.725	0.0	0.0	0.0	0.0
11	2.15	0.0	0.0	0.0	0.0
12	2.925	0.0	0.0	0.0	0.0
13	3.975	0.0	0.0	0.0	0.0
14	7.875	0.0	0.0	0.0	0.0
15	9.1	0.0	0.0	0.0	0.0
16	10.7	0.0	0.0	0.0	0.0
17	14.075	0.0	0.0	0.0	0.0
18	17.35	0.0	0.0	0.0	0.0
19	19.55	0.0	0.0	0.0	0.0
20	23.0	0.0	0.0	0.0	0.0
21	26.25	0.0	0.0	0.0	0.0
22	32.05	0.0	0.0	0.0	0.0
23	40.55	0.0	0.0	0.0	0.0
24	43.125	0.0	0.0	0.0	0.0
25	50.4	0.0	0.0	0.0	0.0
26	54.075	0.0	0.0	0.0	0.0
27	56.825	0.0	0.0	0.0	0.0
28	60.875	0.0	0.0	0.0	0.0
29	63.05	0.0	0.0	0.0	0.0
30	65.275	0.0	0.0	0.0	0.0
31	70.75	0.0	0.0	0.0	0.0
32	72.6	0.0	0.0	0.0	0.0
33	74.95	0.0	0.0	0.0	0.0
34	78.275	0.0	0.0	0.0	0.0
35	81.4	0.0	0.0	0.0	0.0
36	85.8	0.0	0.0	0.0	0.0
37	89.25	0.0	0.0	0.0	0.0
38	90.5	0.0	0.0	0.0	0.0
39	91.3	0.0	0.0	0.0	0.0
40	92.125	0.0	0.0	0.0	0.0
41	92.775	0.0	0.0	0.0	0.0
42	93.75	0.0	0.0	0.0	0.0
43	93.975	0.0	0.0	0.0	0.0
44	94.15	0.0	0.0	0.0	0.0
45	94.375	0.0	0.0	0.0	0.0
46	94.7	0.0	0.0	0.0	0.0
47	94.825	0.0	0.0	0.0	0.0
48	94.975	0.0	0.0	0.0	0.0
49	95.075	0.0	0.0	0.0	0.0
50	95.15	0.0	0.0	0.0	0.0
51	95.2	0.0	0.0	0.0	0.0
52	95.325	0.0	0.0	0.0	0.0
53	95.375	0.0	0.0	0.0	0.0
54	95.375	0.0	0.0	0.0	0.0
55	95.375	0.0	0.0	0.0	0.0
56	95.4	0.0	0.0	0.0	0.0
57	95.425	0.0	0.0	0.0	0.0
58	95.45	0.0	0.0	0.0	0.0
59	95.475	0.0	0.0	0.0	0.0
60	95.475	0.0	0.0	0.0	0.0
61	95.475	0.0	0.0	0.0	0.0
62	95.475	0.0	0.0	0.0	0.0
63	95.475	0.0	0.0	0.0	0.0
64	95.475	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGTCAG	30	7.878043E-8	70.00001	17
TTCAATT	15	0.0020658788	70.00001	11
TTGTAGT	45	3.6379788E-12	70.00001	5
TCAGGAT	15	0.0020658788	70.00001	20
GCTGGTC	15	0.0020658788	70.00001	15
ATTGGTC	15	0.0020658788	70.00001	15
CACCGCC	15	0.0020658788	70.00001	26
GGATTGT	15	0.0020658788	70.00001	2
ACCGCCC	15	0.0020658788	70.00001	27
CAATTGG	15	0.0020658788	70.00001	13
TCAATTG	15	0.0020658788	70.00001	12
CCGTTGT	30	7.878043E-8	70.00001	2
GGTCAGG	15	0.0020658788	70.00001	18
GGTCAGA	15	0.0020658788	70.00001	18
GTTCAAT	15	0.0020658788	70.00001	10
GATTGTA	15	0.0020658788	70.00001	3
CAGAGCA	15	0.0020658788	70.00001	21
CTGGTCA	15	0.0020658788	70.00001	16
ATTGTAG	15	0.0020658788	70.00001	4
TCAGAGC	15	0.0020658788	70.00001	20
>>END_MODULE
Rejected 344832 READS because READLEN < 1
Read 344832 spots for SRR11699293.sra
Written 344832 spots for SRR11699293.sra
Rejected 344832 READS because READLEN < 1
Read 344832 spots for SRR11699293.sra
Written 344832 spots for SRR11699293.sra
Rejected 344832 READS because READLEN < 1
Read 344832 spots for SRR11699293.sra
Written 344832 spots for SRR11699293.sra
Rejected 344832 READS because READLEN < 1
Read 344832 spots for SRR11699293.sra
Written 344832 spots for SRR11699293.sra
Rejected 344832 READS because READLEN < 1
Read 344832 spots for SRR11699293.sra
Written 344832 spots for SRR11699293.sra
Rejected 344832 READS because READLEN < 1
Rejected 344832 READS because READLEN < 1
Read 344832 spots for SRR11699293.sra
Rejected 344832 READS because READLEN < 1
Written 344832 spots for SRR11699293.sra
Read 344832 spots for SRR11699293.sra
Read 344832 spots for SRR11699293.sra
Written 344832 spots for SRR11699293.sra
Written 344832 spots for SRR11699293.sra
Rejected 344832 READS because READLEN < 1
Read 344832 spots for SRR11699293.sra
Written 344832 spots for SRR11699293.sra
Rejected 344832 READS because READLEN < 1
Rejected 344832 READS because READLEN < 1
Read 344832 spots for SRR11699293.sra
Written 344832 spots for SRR11699293.sra
Read 344832 spots for SRR11699293.sra
Written 344832 spots for SRR11699293.sra
Rejected 344832 READS because READLEN < 1
Read 344832 spots for SRR11699293.sra
Written 344832 spots for SRR11699293.sra
Rejected 344834 READS because READLEN < 1
Rejected 344832 READS because READLEN < 1
Rejected 344832 READS because READLEN < 1
Read 344834 spots for SRR11699293.sra
Read 344832 spots for SRR11699293.sra
Read 344832 spots for SRR11699293.sra
Written 344834 spots for SRR11699293.sra
Written 344832 spots for SRR11699293.sra
Written 344832 spots for SRR11699293.sra
Rejected 344832 READS because READLEN < 1
Read 344832 spots for SRR11699293.sra
Written 344832 spots for SRR11699293.sra
Rejected 344832 READS because READLEN < 1
Read 344832 spots for SRR11699293.sra
Written 344832 spots for SRR11699293.sra
Rejected 344832 READS because READLEN < 1
Read 344832 spots for SRR11699293.sra
Written 344832 spots for SRR11699293.sra
Rejected 344832 READS because READLEN < 1
Read 344832 spots for SRR11699293.sra
Written 344832 spots for SRR11699293.sra
Rejected 344832 READS because READLEN < 1
Read 344832 spots for SRR11699293.sra
Written 344832 spots for SRR11699293.sra
SRR ids: ['SRR11699293.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_04gdgycu
SRR11699293.sra spots: 6896642
blocks: [[1, 344832], [344833, 689664], [689665, 1034496], [1034497, 1379328], [1379329, 1724160], [1724161, 2068992], [2068993, 2413824], [2413825, 2758656], [2758657, 3103488], [3103489, 3448320], [3448321, 3793152], [3793153, 4137984], [4137985, 4482816], [4482817, 4827648], [4827649, 5172480], [5172481, 5517312], [5517313, 5862144], [5862145, 6206976], [6206977, 6551808], [6551809, 6896642]]
SRR11699293 file size 1311156
SRR11699293 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR11699293 SRR11699293_1.fastq
Input file:	SRR11699293_1.fastq
trimmed:	SRR11699293-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	20
Wed Jun 18 08:30:28 2025 >> started

Wed Jun 18 08:30:32 2025 >> done (4.271s)
6896642 reads processed; of these:
1274722 (18.48%) short reads filtered out after trimming by size control
   4069 ( 0.06%) empty reads filtered out after trimming by size control
5617851 (81.46%) reads available; of these:
5521651 (98.29%) trimmed reads available after processing
  96200 ( 1.71%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	 152601	  2.72%
 19	 243712	  4.34%
 20	 228003	  4.06%
 21	 421307	  7.50%
 22	 569878	 10.14%
 23	 207857	  3.70%
 24	 535705	  9.54%
 25	 294036	  5.23%
 26	 176482	  3.14%
 27	 295845	  5.27%
 28	 148773	  2.65%
 29	 164225	  2.92%
 30	 357014	  6.35%
 31	 167279	  2.98%
 32	 171493	  3.05%
 33	 235856	  4.20%
 34	 217343	  3.87%
 35	 290268	  5.17%
 36	 235828	  4.20%
 37	  93069	  1.66%
 38	  66815	  1.19%
 39	  58159	  1.04%
 40	  44638	  0.79%
 41	  69835	  1.24%
 42	  33291	  0.59%
 43	  26532	  0.47%
 44	  15805	  0.28%
 45	      0	  0.00%
 46	      0	  0.00%
 47	      0	  0.00%
 48	      0	  0.00%
 49	      0	  0.00%
 50	      0	  0.00%
 51	      0	  0.00%
 52	      0	  0.00%
 53	      0	  0.00%
 54	      0	  0.00%
 55	      0	  0.00%
 56	      0	  0.00%
 57	      0	  0.00%
 58	      0	  0.00%
 59	      0	  0.00%
 60	      0	  0.00%
 61	      0	  0.00%
 62	      0	  0.00%
 63	      0	  0.00%
 64	      0	  0.00%
 65	      0	  0.00%
 66	      0	  0.00%
 67	      0	  0.00%
 68	      0	  0.00%
 69	      0	  0.00%
 70	      0	  0.00%
 71	      0	  0.00%
 72	      0	  0.00%
 73	      0	  0.00%
 74	      0	  0.00%
 75	      2	  0.00%
 76	  96200	  1.71%
5617851 reads passed initial QC


criterion=sequence-density
sequence-density=8.02
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=17
prefix-density=0.01
prefix-fanout=1.0
sequence=TCCGTTGTAGTCTAGCTGGTCAGGATA


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=38
fanout-score=67.27
fanout-score-rank=1
prefix-density=0.41
prefix-fanout=1.0
sequence=CCTCGTGTTTAGTTGCCACTATGAGTTTGGAACCCTGAACAGACCGCCGGTGTTAAGCCGGAGGAAGGAGATCGGAAG
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	Jun 18 08:31:05
                             Started mapping on |	Jun 18 08:31:05
                                    Finished on |	Jun 18 08:31:17
       Mapping speed, Million of reads per hour |	1685.36

                          Number of input reads |	5617851
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	493864
                        Uniquely mapped reads % |	8.79%
                          Average mapped length |	24.95
                       Number of splices: Total |	10613
            Number of splices: Annotated (sjdb) |	4031
                       Number of splices: GT/AG |	9549
                       Number of splices: GC/AG |	763
                       Number of splices: AT/AC |	6
               Number of splices: Non-canonical |	295
                      Mismatch rate per base, % |	1.17%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.42
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.18
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	771309
             % of reads mapped to multiple loci |	13.73%
        Number of reads mapped to too many loci |	1454707
             % of reads mapped to too many loci |	25.89%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.62%
                     % of reads unmapped: other |	44.97%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	4352678	4352678	4352678
N_multimapping	771309	771309	771309
N_noFeature	414731	428871	477198
N_ambiguous	2577	36	21
UnstrandedReadsAssigned:76556 PositiveStrandReadsAssigned:64957 NegativeStrandReadsAssigned:16645
Dataset is classified unstranded
MeadianReadLen=26 20thPercentileLength=22 echo kmer=19
SRR11699293 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 72,539
[index] number of k-mers: 56,563,136
[index] number of equivalence classes: 414,827
[quant] running in single-end mode
[quant] will process file 1: SRR11699293-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 5,617,851 reads, 1,230,767 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 900 rounds

  72540 SRR11699293.ke.tsv
  44303 SRR11699293.se.tsv
 116843 total
==> SRR11699293.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Zm00001eb096110_T001	2067	1968	0	0
Zm00001eb321680_T001	3417	3318	5	1.48601
Zm00001eb323640_T001	3273	3174	1	0.310686
Zm00001eb314140_T001	893	794	0	0
Zm00001eb080260_T001	3529	3430	0	0
Zm00001eb026490_T001	2433	2334	0	0
Zm00001eb026490_T002	2229	2130	0	0
Zm00001eb026490_T003	2220	2121	0	0
Zm00001eb281360_T001	2412	2313	0	0

==> SRR11699293.se.tsv <==
Zm00001eb015280	0
Zm00001eb000610	0
Zm00001eb033210	0
Zm00001eb044610	0
Zm00001eb014360	0
Zm00001eb051320	0
Zm00001eb021860	0
Zm00001eb011690	0
Zm00001eb016840	0
Zm00001eb052060	0
SRR11699293 completed mapping pipeline successfully
