Starting /dee2/code/volunteer_pipeline.sh SRR11699329
    current disk space = 2465293783040
    free memory = 1564528880 
SRR11699329 SRAfilesize
097ef305355711e65f21edaa93c0bccf  SRR11699329.sra
SRR11699329.sra file validated
SRR11699329 is single end
SRR11699329 is conventional basespace
SRR11699329 read1 length is 76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR11699329_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.093	32.0	32.0	32.0	32.0	32.0
2	31.39025	32.0	32.0	32.0	32.0	32.0
3	31.4315	32.0	32.0	32.0	32.0	32.0
4	31.5315	32.0	32.0	32.0	32.0	32.0
5	31.54225	32.0	32.0	32.0	32.0	32.0
6	35.061	36.0	36.0	36.0	36.0	36.0
7	35.1465	36.0	36.0	36.0	36.0	36.0
8	35.016	36.0	36.0	36.0	36.0	36.0
9	34.86075	36.0	36.0	36.0	32.0	36.0
10-11	35.080749999999995	36.0	36.0	36.0	36.0	36.0
12-13	35.039	36.0	36.0	36.0	36.0	36.0
14-15	34.981750000000005	36.0	36.0	36.0	36.0	36.0
16-17	35.0875	36.0	36.0	36.0	36.0	36.0
18-19	35.095124999999996	36.0	36.0	36.0	36.0	36.0
20-21	34.93925	36.0	36.0	36.0	36.0	36.0
22-23	34.989999999999995	36.0	36.0	36.0	36.0	36.0
24-25	34.98625	36.0	36.0	36.0	36.0	36.0
26-27	34.89375	36.0	36.0	36.0	34.0	36.0
28-29	34.8865	36.0	36.0	36.0	36.0	36.0
30-31	34.856750000000005	36.0	36.0	36.0	34.0	36.0
32-33	34.869125	36.0	36.0	36.0	36.0	36.0
34-35	34.9385	36.0	36.0	36.0	36.0	36.0
36-37	34.868	36.0	36.0	36.0	36.0	36.0
38-39	34.76625	36.0	36.0	36.0	34.0	36.0
40-41	34.832125	36.0	36.0	36.0	36.0	36.0
42-43	34.849875	36.0	36.0	36.0	34.0	36.0
44-45	34.804	36.0	36.0	36.0	32.0	36.0
46-47	34.84125	36.0	36.0	36.0	34.0	36.0
48-49	34.7685	36.0	36.0	36.0	34.0	36.0
50-51	34.543875	36.0	36.0	36.0	32.0	36.0
52-53	34.388374999999996	36.0	36.0	36.0	32.0	36.0
54-55	34.241375000000005	36.0	36.0	36.0	32.0	36.0
56-57	33.72725	36.0	36.0	36.0	27.0	36.0
58-59	33.330375000000004	36.0	34.0	36.0	27.0	36.0
60-61	33.035875	36.0	34.0	36.0	20.5	36.0
62-63	32.797375	36.0	32.0	36.0	21.0	36.0
64-65	32.378375	36.0	32.0	36.0	14.0	36.0
66-67	31.438875	36.0	32.0	36.0	14.0	36.0
68-69	31.570125	36.0	32.0	36.0	14.0	36.0
70-71	31.740875	36.0	32.0	36.0	14.0	36.0
72-73	31.995124999999998	36.0	32.0	36.0	14.0	36.0
74-75	32.515375	36.0	32.0	36.0	14.0	36.0
76	28.84475	32.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	2.0
22	3.0
23	3.0
24	9.0
25	19.0
26	26.0
27	43.0
28	73.0
29	100.0
30	147.0
31	154.0
32	251.0
33	428.0
34	1138.0
35	1603.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.3	30.3	21.65	22.75
2	20.775	27.575	26.900000000000002	24.75
3	24.4	32.525	17.375	25.7
4	28.075	34.175	17.675	20.075000000000003
5	19.675	25.474999999999998	25.025	29.825000000000003
6	26.700000000000003	26.724999999999998	26.525	20.05
7	32.275	35.375	16.775000000000002	15.575
8	20.525	28.625	29.75	21.099999999999998
9	24.3	33.45	18.425	23.825
10-11	31.6	27.35	24.099999999999998	16.950000000000003
12-13	21.762500000000003	24.5125	23.325000000000003	30.4
14-15	29.4875	36.8375	18.125	15.55
16-17	22.9875	24.075	32.6625	20.275000000000002
18-19	36.662499999999994	24.8	22.6	15.937499999999998
20-21	21.65	36.0375	24.5	17.8125
22-23	30.9875	36.9625	14.725	17.325
24-25	28.125	38.9125	15.725	17.2375
26-27	27.800000000000004	32.725	19.3125	20.1625
28-29	32.2625	27.224999999999998	17.8125	22.7
30-31	32.7125	36.512499999999996	11.05	19.725
32-33	29.975	40.0	10.4375	19.5875
34-35	31.4	31.25	13.200000000000001	24.15
36-37	28.237499999999997	32.6375	10.487499999999999	28.6375
38-39	23.35	36.475	12.0375	28.1375
40-41	25.1875	31.362499999999997	16.2375	27.212500000000002
42-43	26.887499999999996	24.675	20.8625	27.575
44-45	21.712500000000002	32.025	16.075	30.1875
46-47	20.925	32.6625	15.187500000000002	31.225
48-49	16.6	27.437499999999996	19.375	36.5875
50-51	14.9875	27.3375	20.825	36.85
52-53	20.575	26.224999999999998	20.1875	33.0125
54-55	17.9	29.362500000000004	20.1625	32.574999999999996
56-57	15.187500000000002	25.074999999999996	23.8625	35.875
58-59	18.425	26.187500000000004	20.837500000000002	34.55
60-61	19.775000000000002	31.125000000000004	20.5	28.599999999999998
62-63	19.6125	32.9625	20.875	26.55
64-65	18.55	24.8125	26.5875	30.049999999999997
66-67	18.75	17.849999999999998	30.2875	33.1125
68-69	19.4875	21.3625	30.85	28.299999999999997
70-71	22.175	20.5625	32.300000000000004	24.962500000000002
72-73	21.6875	17.962500000000002	30.2125	30.1375
74-75	24.325	13.212499999999999	29.862499999999997	32.6
76	18.45	12.025	38.224999999999994	31.3
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.5
23	0.5
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.5
32	0.5
33	0.0
34	0.5
35	1.0
36	1.0
37	1.0
38	2.5
39	6.5
40	13.0
41	24.0
42	31.0
43	43.5
44	82.0
45	147.0
46	186.0
47	311.5
48	703.5
49	811.5
50	653.0
51	575.0
52	454.0
53	345.0
54	279.0
55	222.5
56	123.5
57	70.5
58	60.0
59	37.5
60	10.0
61	4.0
62	3.0
63	2.0
64	1.0
65	1.0
66	0.5
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.5
81	0.5
82	0.0
83	0.0
84	0.0
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.95
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.41493456505005	58.074999999999996
2	4.926866820631255	6.4
3	1.6551193225558123	3.225
4	1.0392609699769053	2.7
5	0.8468052347959968	2.75
6	0.4234026173979984	1.6500000000000001
7	0.26943802925327176	1.225
8	0.23094688221709006	1.2
9	0.03849114703618168	0.22499999999999998
>10	1.077752117013087	14.025000000000002
>50	0.03849114703618168	1.925
>100	0.03849114703618168	6.6000000000000005
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCC	264	6.6000000000000005	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAAC	77	1.925	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCA	49	1.225	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	44	1.0999999999999999	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCGTTGTAGTCTAGCTGGTCAGGATACTCAGATCGGAAGAGCACACGTC	38	0.95	No Hit
AACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAG	33	0.8250000000000001	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGT	30	0.75	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTGAGATCGGAAGAGCAC	25	0.625	No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCAGATCGGAAGAGCACACG	24	0.6	No Hit
TCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACT	24	0.6	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	23	0.575	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACT	19	0.475	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	19	0.475	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCAGATCGGAAGAGCACACGTC	18	0.44999999999999996	No Hit
CTAACGAACGAACGATTTGAAGATCGGAAGAGCACACGTCTGAACTCCAG	18	0.44999999999999996	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCA	17	0.42500000000000004	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAATTCATGATCTGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	17	0.42500000000000004	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGA	15	0.375	TruSeq Adapter, Index 10 (97% over 36bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCG	15	0.375	TruSeq Adapter, Index 10 (97% over 36bp)
CCAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGT	14	0.35000000000000003	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGCAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGC	13	0.325	No Hit
AAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	13	0.325	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCAC	13	0.325	No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATACTCAGATCGGAAGAGCACACGTC	13	0.325	No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACTCAGATCGGAAGAGCACACGTC	12	0.3	No Hit
TGAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGT	12	0.3	No Hit
GCGGCAAAATAGCTCGATGCCAGAATAGATCGGAAGAGCACACGTCTGAA	12	0.3	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGGCTATAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCG	11	0.27499999999999997	TruSeq Adapter, Index 10 (97% over 36bp)
GGAGGTCCTGCGGCAAAATAGCTCGATGCCAGAATAGATCGGAAGAGCAC	10	0.25	No Hit
CCTGCGGCAAAATAGCTCGATGCCAGAATAGATCGGAAGAGCACACGTCT	10	0.25	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT	9	0.22499999999999998	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCGTTGTAGTCTAGTTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GACACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACTC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTAACGAACGAACGATTTGAATAGATCGGAAGAGCACACGTCTGAACTCC	8	0.2	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCGCCATGGTGAAATTGGTAGACACGCTGCTCTTAGATCGGAAGAGCAC	8	0.2	No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCAGATCGGAAGAGCACAC	8	0.2	No Hit
CACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACTCCA	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACACGACTCTCGGCAACGAGATCGGAAGAGCACACGTCTGAACTCCAG	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGT	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGATGTAGCTCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC	7	0.17500000000000002	TruSeq Adapter, Index 5 (97% over 35bp)
ATATAAGTAGTAAGCCCACCCCAAGATGAGTGCTCTCTCAGATCGGAAGA	7	0.17500000000000002	No Hit
GCAAAATAGCTCGATGCCAGAATAGATCGGAAGAGCACACGTCTGAACTC	7	0.17500000000000002	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCAGATCGGAAGAGC	7	0.17500000000000002	No Hit
ATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGAT	6	0.15	TruSeq Adapter, Index 10 (97% over 36bp)
CGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC	6	0.15	TruSeq Adapter, Index 5 (97% over 35bp)
AAAATAGCTCGATGCCAGAATAGATCGGAAGAGCACACGTCTGAACTCCA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCGAGCGTAGTTCAGTGGTAAAACATCTCCTTGCCAAGGAGAGATCGGAA	6	0.15	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCAGATCGGAAGAGCACAC	6	0.15	No Hit
CATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAA	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACCCCAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTCTGAACTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCACCAGTGGTCTAGTGGTAGAATAAGATCGGAAGAGCACACGTCTGAAC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGAGATCGGAAGAGCACACGT	6	0.15	No Hit
GAGGCATCCTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTC	6	0.15	No Hit
GCGACCCCAGGTCAGTCGGGACTAGATCGGAAGAGCACACGTCTGAACTC	6	0.15	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGATGTATGCAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATC	5	0.125	No Hit
AACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCACCAGTAGTCTAGTGGTAGAATAGTACCAGATCGGAAGAGCACACGTC	5	0.125	No Hit
ACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAAGGATCATTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGA	5	0.125	TruSeq Adapter, Index 10 (97% over 36bp)
CTGCGGCAAAATAGCTCGATGCCAGAATAGATCGGAAGAGCACACGTCTG	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCTAGATCGGAAGAGCA	5	0.125	No Hit
GACACGACTCTCGGCAACGGATATCTCGGCAGATCGGAAGAGCACACGTC	5	0.125	No Hit
GACACGACTCTCGGCAACGGATATCAGATCGGAAGAGCACACGTCTGAAC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCCTAACGAACGAACGATTTGAAGATCGGAAGAGCACACGTCTGAACTC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACTCC	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGACACGACTCTCGGCAACGGATATCTCGGCAGATCGGAAGAGCACACGT	5	0.125	No Hit
GTAAGCCCACCCCAAGATGAGTGCTCTCTCAGATCGGAAGAGCACACGTC	5	0.125	No Hit
ACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAA	5	0.125	TruSeq Adapter, Index 10 (97% over 36bp)
GTCAGGATAGCTCAGTTGGTAGAGCAGAGGACTGAGATCGGAAGAGCACA	5	0.125	No Hit
GACACGACTCTCGGCAACGGATATCTCGAGATCGGAAGAGCACACGTCTG	5	0.125	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACCTGGTTGATCCTGCCAGTAGTCATATGCTTGAGATCGGAAGAGCACA	5	0.125	No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCCTAGATCGGAAGAGCACA	5	0.125	No Hit
GAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCG	5	0.125	TruSeq Adapter, Index 10 (97% over 36bp)
GACACGACTCTCGGCAACGGATATCTCGGCTAGATCGGAAGAGCACACGT	5	0.125	No Hit
GATGTATGCAGCTGAGGCATCCTAACGAACGAACGATTTGAACAGATCGG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.025	0.0	0.0	0.0	0.0
2	0.025	0.0	0.0	0.0	0.0
3	0.05	0.0	0.0	0.0	0.0
4	0.05	0.0	0.0	0.0	0.0
5	0.05	0.0	0.0	0.0	0.0
6	0.05	0.0	0.0	0.0	0.0
7	0.1	0.0	0.0	0.0	0.0
8	0.125	0.0	0.0	0.0	0.0
9	0.4	0.0	0.0	0.0	0.0
10	0.8	0.0	0.0	0.0	0.0
11	1.0	0.0	0.0	0.0	0.0
12	1.65	0.0	0.0	0.0	0.0
13	2.525	0.0	0.0	0.0	0.0
14	4.125	0.0	0.0	0.0	0.0
15	5.4	0.0	0.0	0.0	0.0
16	6.8	0.0	0.0	0.0	0.0
17	9.625	0.0	0.0	0.0	0.0
18	12.575	0.0	0.0	0.0	0.0
19	14.45	0.0	0.0	0.0	0.0
20	17.575	0.0	0.0	0.0	0.0
21	21.1	0.0	0.0	0.0	0.0
22	25.975	0.0	0.0	0.0	0.0
23	36.7	0.0	0.0	0.0	0.0
24	40.3	0.0	0.0	0.0	0.0
25	49.775	0.0	0.0	0.0	0.0
26	54.25	0.0	0.0	0.0	0.0
27	57.025	0.0	0.0	0.0	0.0
28	60.475	0.0	0.0	0.0	0.0
29	62.875	0.0	0.0	0.0	0.0
30	65.475	0.0	0.0	0.0	0.0
31	70.925	0.0	0.0	0.0	0.0
32	73.9	0.0	0.0	0.0	0.0
33	76.65	0.0	0.0	0.0	0.0
34	78.925	0.0	0.0	0.0	0.0
35	81.275	0.0	0.0	0.0	0.0
36	84.425	0.0	0.0	0.0	0.0
37	86.675	0.0	0.0	0.0	0.0
38	88.7	0.0	0.0	0.0	0.0
39	89.725	0.0	0.0	0.0	0.0
40	90.95	0.0	0.0	0.0	0.0
41	91.7	0.0	0.0	0.0	0.0
42	92.475	0.0	0.0	0.0	0.0
43	93.0	0.0	0.0	0.0	0.0
44	93.625	0.0	0.0	0.0	0.0
45	94.025	0.0	0.0	0.0	0.0
46	94.475	0.0	0.0	0.0	0.0
47	94.825	0.0	0.0	0.0	0.0
48	94.925	0.0	0.0	0.0	0.0
49	95.2	0.0	0.0	0.0	0.0
50	95.2	0.0	0.0	0.0	0.0
51	95.275	0.0	0.0	0.0	0.0
52	95.325	0.0	0.0	0.0	0.0
53	95.325	0.0	0.0	0.0	0.0
54	95.35	0.0	0.0	0.0	0.0
55	95.375	0.0	0.0	0.0	0.0
56	95.375	0.0	0.0	0.0	0.0
57	95.375	0.0	0.0	0.0	0.0
58	95.375	0.0	0.0	0.0	0.0
59	95.375	0.0	0.0	0.0	0.0
60	95.375	0.0	0.0	0.0	0.0
61	95.375	0.0	0.0	0.0	0.0
62	95.375	0.0	0.0	0.0	0.0
63	95.375	0.0	0.0	0.0	0.0
64	95.375	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATATCT	15	0.0020658788	70.00001	20
AGTGGTA	15	0.0020658788	70.00001	14
CGGATAT	15	0.0020658788	70.00001	18
GGATATC	15	0.0020658788	70.00001	19
TATCTCG	15	0.0020658788	70.00001	22
ATATCTC	15	0.0020658788	70.00001	21
TCTAGCT	20	7.003785E-5	70.0	11
TTGTAGT	25	2.3570374E-6	70.0	5
TCAGGAT	20	7.003785E-5	70.0	20
GCTGGTC	20	7.003785E-5	70.0	15
TAGCTGG	20	7.003785E-5	70.0	13
CCGTTGT	20	7.003785E-5	70.0	2
TAGTCTA	20	7.003785E-5	70.0	8
GGTCAGG	20	7.003785E-5	70.0	18
CTGGTCA	20	7.003785E-5	70.0	16
TGTAGTC	25	2.3570374E-6	70.0	6
CTAGCTG	20	7.003785E-5	70.0	12
GTCTAGC	20	7.003785E-5	70.0	10
TCCGTTG	20	7.003785E-5	70.0	1
CGTTGTA	20	7.003785E-5	70.0	3
>>END_MODULE
Rejected 362705 READS because READLEN < 1
Read 362705 spots for SRR11699329.sra
Written 362705 spots for SRR11699329.sra
Rejected 362705 READS because READLEN < 1
Read 362705 spots for SRR11699329.sra
Written 362705 spots for SRR11699329.sra
Rejected 362705 READS because READLEN < 1
Read 362705 spots for SRR11699329.sra
Written 362705 spots for SRR11699329.sra
Rejected 362705 READS because READLEN < 1
Read 362705 spots for SRR11699329.sra
Written 362705 spots for SRR11699329.sra
Rejected 362705 READS because READLEN < 1
Read 362705 spots for SRR11699329.sra
Written 362705 spots for SRR11699329.sra
Rejected 362714 READS because READLEN < 1
Read 362714 spots for SRR11699329.sra
Written 362714 spots for SRR11699329.sra
Rejected 362705 READS because READLEN < 1
Read 362705 spots for SRR11699329.sra
Written 362705 spots for SRR11699329.sra
Rejected 362705 READS because READLEN < 1
Read 362705 spots for SRR11699329.sra
Written 362705 spots for SRR11699329.sra
Rejected 362705 READS because READLEN < 1
Read 362705 spots for SRR11699329.sra
Written 362705 spots for SRR11699329.sra
Rejected 362705 READS because READLEN < 1
Read 362705 spots for SRR11699329.sra
Written 362705 spots for SRR11699329.sra
Rejected 362705 READS because READLEN < 1
Read 362705 spots for SRR11699329.sra
Written 362705 spots for SRR11699329.sra
Rejected 362705 READS because READLEN < 1
Read 362705 spots for SRR11699329.sra
Written 362705 spots for SRR11699329.sra
Rejected 362705 READS because READLEN < 1
Read 362705 spots for SRR11699329.sra
Written 362705 spots for SRR11699329.sra
Rejected 362705 READS because READLEN < 1
Read 362705 spots for SRR11699329.sra
Written 362705 spots for SRR11699329.sra
Rejected 362705 READS because READLEN < 1
Read 362705 spots for SRR11699329.sra
Written 362705 spots for SRR11699329.sra
Rejected 362705 READS because READLEN < 1
Read 362705 spots for SRR11699329.sra
Written 362705 spots for SRR11699329.sra
Rejected 362705 READS because READLEN < 1
Read 362705 spots for SRR11699329.sra
Written 362705 spots for SRR11699329.sra
Rejected 362705 READS because READLEN < 1
Read 362705 spots for SRR11699329.sra
Written 362705 spots for SRR11699329.sra
Rejected 362705 READS because READLEN < 1
Read 362705 spots for SRR11699329.sra
Written 362705 spots for SRR11699329.sra
Rejected 362705 READS because READLEN < 1
Read 362705 spots for SRR11699329.sra
Written 362705 spots for SRR11699329.sra
SRR ids: ['SRR11699329.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_dg9dc7t4
SRR11699329.sra spots: 7254109
blocks: [[1, 362705], [362706, 725410], [725411, 1088115], [1088116, 1450820], [1450821, 1813525], [1813526, 2176230], [2176231, 2538935], [2538936, 2901640], [2901641, 3264345], [3264346, 3627050], [3627051, 3989755], [3989756, 4352460], [4352461, 4715165], [4715166, 5077870], [5077871, 5440575], [5440576, 5803280], [5803281, 6165985], [6165986, 6528690], [6528691, 6891395], [6891396, 7254109]]
SRR11699329 file size 1379228
SRR11699329 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR11699329 SRR11699329_1.fastq
Input file:	SRR11699329_1.fastq
trimmed:	SRR11699329-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	20
Wed Jun 18 14:54:44 2025 >> started

Wed Jun 18 14:58:33 2025 >> done (229.562s)
7254109 reads processed; of these:
1172014 (16.16%) short reads filtered out after trimming by size control
   4292 ( 0.06%) empty reads filtered out after trimming by size control
6077803 (83.78%) reads available; of these:
5946061 (97.83%) trimmed reads available after processing
 131742 ( 2.17%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	 158710	  2.61%
 19	 248480	  4.09%
 20	 269286	  4.43%
 21	 373224	  6.14%
 22	 730674	 12.02%
 23	 268763	  4.42%
 24	 715421	 11.77%
 25	 328555	  5.41%
 26	 206535	  3.40%
 27	 242268	  3.99%
 28	 183715	  3.02%
 29	 192276	  3.16%
 30	 375714	  6.18%
 31	 211389	  3.48%
 32	 203771	  3.35%
 33	 184051	  3.03%
 34	 169476	  2.79%
 35	 212730	  3.50%
 36	 155693	  2.56%
 37	 107755	  1.77%
 38	  81655	  1.34%
 39	  82445	  1.36%
 40	  58800	  0.97%
 41	  72807	  1.20%
 42	  46449	  0.76%
 43	  40192	  0.66%
 44	  25221	  0.41%
 45	      0	  0.00%
 46	      0	  0.00%
 47	      0	  0.00%
 48	      0	  0.00%
 49	      0	  0.00%
 50	      0	  0.00%
 51	      0	  0.00%
 52	      0	  0.00%
 53	      0	  0.00%
 54	      0	  0.00%
 55	      0	  0.00%
 56	      0	  0.00%
 57	      0	  0.00%
 58	      0	  0.00%
 59	      0	  0.00%
 60	      0	  0.00%
 61	      0	  0.00%
 62	      0	  0.00%
 63	      0	  0.00%
 64	      0	  0.00%
 65	      0	  0.00%
 66	      0	  0.00%
 67	      0	  0.00%
 68	      0	  0.00%
 69	      0	  0.00%
 70	      0	  0.00%
 71	      0	  0.00%
 72	      0	  0.00%
 73	      0	  0.00%
 74	      0	  0.00%
 75	      6	  0.00%
 76	 131742	  2.17%
6077803 reads passed initial QC


criterion=sequence-density
sequence-density=4.94
sequence-density-rank=1
fanout-score=1.00
fanout-score-rank=17
prefix-density=0.01
prefix-fanout=1.0
sequence=TCCGTTGTAGTCTAGCTGGTCAGGATA


criterion=fanout-score
sequence-density=0.20
sequence-density-rank=23
fanout-score=40.24
fanout-score-rank=1
prefix-density=0.89
prefix-fanout=8.9
sequence=TAGCTCAGTGGTAGAGC
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	Jun 18 15:04:08
                             Started mapping on |	Jun 18 15:04:37
                                    Finished on |	Jun 18 15:09:38
       Mapping speed, Million of reads per hour |	72.69

                          Number of input reads |	6077803
                      Average input read length |	28
                                    UNIQUE READS:
                   Uniquely mapped reads number |	654720
                        Uniquely mapped reads % |	10.77%
                          Average mapped length |	24.36
                       Number of splices: Total |	13951
            Number of splices: Annotated (sjdb) |	5423
                       Number of splices: GT/AG |	13072
                       Number of splices: GC/AG |	596
                       Number of splices: AT/AC |	7
               Number of splices: Non-canonical |	276
                      Mismatch rate per base, % |	1.00%
                         Deletion rate per base |	0.01%
                        Deletion average length |	1.52
                        Insertion rate per base |	0.00%
                       Insertion average length |	1.07
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	793202
             % of reads mapped to multiple loci |	13.05%
        Number of reads mapped to too many loci |	1163771
             % of reads mapped to too many loci |	19.15%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	7.97%
                     % of reads unmapped: other |	49.06%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	4629881	4629881	4629881
N_multimapping	793202	793202	793202
N_noFeature	556287	573586	634354
N_ambiguous	3166	51	55
UnstrandedReadsAssigned:95267 PositiveStrandReadsAssigned:81083 NegativeStrandReadsAssigned:20311
Dataset is classified unstranded
MeadianReadLen=25 20thPercentileLength=22 echo kmer=19
SRR11699329 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 72,539
[index] number of k-mers: 56,563,136
[index] number of equivalence classes: 414,827
[quant] running in single-end mode
[quant] will process file 1: SRR11699329-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 6,077,803 reads, 1,204,639 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 895 rounds

  72540 SRR11699329.ke.tsv
  44303 SRR11699329.se.tsv
 116843 total
==> SRR11699329.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Zm00001eb096110_T001	2067	1968	0	0
Zm00001eb321680_T001	3417	3318	4	1.08711
Zm00001eb323640_T001	3273	3174	0	0
Zm00001eb314140_T001	893	794	0	0
Zm00001eb080260_T001	3529	3430	0	0
Zm00001eb026490_T001	2433	2334	0	0
Zm00001eb026490_T002	2229	2130	0	0
Zm00001eb026490_T003	2220	2121	0	0
Zm00001eb281360_T001	2412	2313	0	0

==> SRR11699329.se.tsv <==
Zm00001eb015280	0
Zm00001eb000610	0
Zm00001eb033210	0
Zm00001eb044610	0
Zm00001eb014360	0
Zm00001eb051320	0
Zm00001eb021860	0
Zm00001eb011690	0
Zm00001eb016840	0
Zm00001eb052060	0
SRR11699329 completed mapping pipeline successfully
