Starting /dee2/code/volunteer_pipeline.sh SRR11699706
    current disk space = 2452311642112
    free memory = 1581588188 
SRR11699706 SRAfilesize
6a624a3bab1826d13f22b897d94ec98f  SRR11699706.sra
SRR11699706.sra file validated
SRR11699706 is single end
SRR11699706 is conventional basespace
SRR11699706 read1 length is 76 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR11699706_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	253
Sequences flagged as poor quality	0
Sequence length	76
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.138339920948617	32.0	32.0	32.0	21.0	32.0
2	30.359683794466402	32.0	32.0	32.0	21.0	32.0
3	30.64426877470356	32.0	32.0	32.0	32.0	32.0
4	30.841897233201582	32.0	32.0	32.0	32.0	32.0
5	30.217391304347824	32.0	32.0	32.0	21.0	32.0
6	33.28853754940712	36.0	36.0	36.0	21.0	36.0
7	33.28853754940712	36.0	36.0	36.0	14.0	36.0
8	33.466403162055336	36.0	36.0	36.0	21.0	36.0
9	32.569169960474305	36.0	36.0	36.0	14.0	36.0
10-11	32.798418972332016	36.0	34.0	36.0	17.5	36.0
12-13	32.11067193675889	36.0	34.0	36.0	14.0	36.0
14-15	32.55731225296443	36.0	34.0	36.0	17.5	36.0
16-17	32.25889328063241	36.0	32.0	36.0	14.0	36.0
18-19	33.08102766798419	36.0	36.0	36.0	17.5	36.0
20-21	31.622529644268774	36.0	32.0	36.0	14.0	36.0
22-23	31.837944664031617	36.0	34.0	36.0	14.0	36.0
24-25	31.824110671936758	36.0	32.0	36.0	14.0	36.0
26-27	30.031620553359684	36.0	20.5	36.0	14.0	36.0
28-29	29.711462450592887	36.0	17.5	36.0	14.0	36.0
30-31	30.729249011857707	36.0	26.5	36.0	14.0	36.0
32-33	29.73913043478261	36.0	17.5	36.0	14.0	36.0
34-35	29.6501976284585	36.0	17.5	36.0	14.0	36.0
36-37	29.561264822134387	36.0	17.5	36.0	14.0	36.0
38-39	30.25296442687747	36.0	27.0	36.0	14.0	36.0
40-41	29.492094861660078	36.0	17.5	36.0	14.0	36.0
42-43	29.50592885375494	36.0	14.0	36.0	14.0	36.0
44-45	29.213438735177867	36.0	14.0	36.0	14.0	36.0
46-47	28.90513833992095	36.0	14.0	36.0	14.0	36.0
48-49	28.14624505928854	36.0	14.0	36.0	14.0	36.0
50-51	27.608695652173914	34.0	14.0	36.0	14.0	36.0
52-53	27.48616600790514	36.0	14.0	36.0	14.0	36.0
54-55	27.59486166007905	36.0	14.0	36.0	14.0	36.0
56-57	27.50395256916996	34.0	14.0	36.0	14.0	36.0
58-59	28.225296442687746	32.0	14.0	36.0	14.0	36.0
60-61	27.007905138339922	32.0	14.0	36.0	14.0	36.0
62-63	25.652173913043477	29.5	14.0	36.0	14.0	36.0
64-65	26.458498023715414	32.0	14.0	36.0	14.0	36.0
66-67	25.23517786561265	29.5	14.0	36.0	14.0	36.0
68-69	26.58300395256917	32.0	14.0	36.0	14.0	36.0
70-71	26.81818181818182	32.0	14.0	36.0	14.0	36.0
72-73	27.507905138339922	34.0	14.0	36.0	14.0	36.0
74-75	27.715415019762844	34.0	14.0	36.0	14.0	36.0
76	26.624505928853754	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	9.0
22	15.0
23	16.0
24	16.0
25	18.0
26	11.0
27	13.0
28	12.0
29	13.0
30	12.0
31	14.0
32	18.0
33	20.0
34	31.0
35	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.158102766798418	20.158102766798418	48.22134387351779	11.462450592885375
2	14.624505928853754	18.181818181818183	32.015810276679844	35.177865612648226
3	18.57707509881423	17.391304347826086	34.387351778656125	29.64426877470356
4	43.08300395256917	14.229249011857709	32.015810276679844	10.67193675889328
5	10.276679841897234	25.691699604743086	52.569169960474305	11.462450592885375
6	12.25296442687747	16.99604743083004	59.683794466403164	11.067193675889328
7	35.96837944664031	13.438735177865613	35.573122529644266	15.019762845849801
8	7.905138339920949	19.367588932806324	60.86956521739131	11.857707509881422
9	11.462450592885375	41.89723320158103	35.177865612648226	11.462450592885375
10-11	29.64426877470356	18.972332015810274	35.573122529644266	15.810276679841898
12-13	13.24110671936759	18.774703557312254	40.51383399209486	27.4703557312253
14-15	27.27272727272727	37.74703557312253	25.494071146245062	9.486166007905137
16-17	10.869565217391305	25.494071146245062	41.89723320158103	21.73913043478261
18-19	38.537549407114625	19.367588932806324	24.308300395256918	17.786561264822133
20-21	18.57707509881423	27.27272727272727	36.16600790513834	17.984189723320156
22-23	30.632411067193676	35.96837944664031	22.92490118577075	10.474308300395258
24-25	25.296442687747035	38.14229249011858	24.703557312252965	11.857707509881422
26-27	14.624505928853754	39.52569169960474	30.237154150197625	15.612648221343871
28-29	21.34387351778656	33.794466403162055	25.889328063241106	18.972332015810274
30-31	30.237154150197625	29.64426877470356	21.73913043478261	18.379446640316203
32-33	20.75098814229249	31.818181818181817	23.122529644268774	24.308300395256918
34-35	28.26086956521739	35.77075098814229	23.715415019762844	12.25296442687747
36-37	24.50592885375494	41.10671936758894	17.193675889328063	17.193675889328063
38-39	19.367588932806324	33.99209486166008	22.92490118577075	23.715415019762844
40-41	13.438735177865613	33.59683794466403	18.972332015810274	33.99209486166008
42-43	23.3201581027668	34.980237154150196	22.529644268774703	19.1699604743083
44-45	16.99604743083004	25.09881422924901	29.051383399209485	28.85375494071146
46-47	14.624505928853754	36.56126482213439	24.308300395256918	24.50592885375494
48-49	17.391304347826086	33.39920948616601	29.249011857707508	19.960474308300398
50-51	10.869565217391305	38.14229249011858	25.889328063241106	25.09881422924901
52-53	13.636363636363635	28.458498023715418	29.64426877470356	28.26086956521739
54-55	14.624505928853754	30.632411067193676	27.66798418972332	27.07509881422925
56-57	13.636363636363635	35.37549407114625	25.889328063241106	25.09881422924901
58-59	13.24110671936759	27.865612648221344	27.27272727272727	31.620553359683797
60-61	11.067193675889328	38.14229249011858	27.27272727272727	23.517786561264824
62-63	16.40316205533597	30.039525691699602	31.818181818181817	21.73913043478261
64-65	10.869565217391305	31.818181818181817	27.865612648221344	29.446640316205535
66-67	15.019762845849801	23.91304347826087	38.3399209486166	22.727272727272727
68-69	10.079051383399209	30.632411067193676	34.980237154150196	24.308300395256918
70-71	12.648221343873518	21.14624505928854	38.93280632411067	27.27272727272727
72-73	20.158102766798418	24.703557312252965	31.620553359683797	23.517786561264824
74-75	12.450592885375494	25.09881422924901	37.94466403162055	24.50592885375494
76	7.5098814229249005	22.529644268774703	48.616600790513836	21.34387351778656
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	5.0
2	2.5
3	0.5
4	0.0
5	1.0
6	2.0
7	3.0
8	4.0
9	2.5
10	1.5
11	1.5
12	1.0
13	0.5
14	0.5
15	2.0
16	3.0
17	3.0
18	1.5
19	0.5
20	2.0
21	3.0
22	3.5
23	4.0
24	5.5
25	7.0
26	5.0
27	4.5
28	4.0
29	2.0
30	1.5
31	2.5
32	5.0
33	6.0
34	4.0
35	3.5
36	3.5
37	2.0
38	3.5
39	3.5
40	3.0
41	6.5
42	9.0
43	11.0
44	11.0
45	11.5
46	14.0
47	19.0
48	22.0
49	16.0
50	12.0
51	17.0
52	16.0
53	10.5
54	11.0
55	9.5
56	6.5
57	4.0
58	3.0
59	1.5
60	0.5
61	0.5
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	253.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.16600790513834
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.78899082568807	83.399209486166
2	0.9174311926605505	1.5810276679841897
3	0.9174311926605505	2.371541501976284
4	0.45871559633027525	1.5810276679841897
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.9174311926605505	11.067193675889328
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	18	7.114624505928854	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10	3.9525691699604746	No Hit
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT	4	1.5810276679841897	TruSeq Adapter, Index 8 (97% over 36bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAA	3	1.185770750988142	TruSeq Adapter, Index 5 (97% over 36bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAT	3	1.185770750988142	TruSeq Adapter, Index 8 (97% over 36bp)
CACGACTCTCGGCAACGGATATCTAGATCGGAAGAGCACACGTCTGAACT	2	0.7905138339920948	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTT	2	0.7905138339920948	TruSeq Adapter, Index 9 (97% over 36bp)
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAA	1	0.3952569169960474	No Hit
TCGGAAGTAGAACAGATCCAAAAAGAACACAACAGAACACCACTCAAAAA	1	0.3952569169960474	No Hit
TGGGACAGTGGCTGTCTTGATGATCTGAATGCAAGGGAATCTGACACGGT	1	0.3952569169960474	No Hit
GAAGTAATTGGTGGCGACGTAGTAGAAGCATGAGGCTATAATGATGTCAC	1	0.3952569169960474	No Hit
GACTGGTAATGAGTGGACTCGCCCAGATCGGAAGAGCACACGTCTGAACT	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTTATTTTACTTATTCCGTGGGTCGGAAGCGGGGCACCAGATCCGAAGA	1	0.3952569169960474	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAATTTTTTTTTT	1	0.3952569169960474	No Hit
ATGGTATACAGTCGCTGGGTTGTAGATCGGAAGAGCACACGTCTGAACTC	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAGGATGGAAGAGCACACGTCTGAA	1	0.3952569169960474	No Hit
CCCCCCCCGACGTCCCGAGATCGGAAGAGCACACTTCTGAACTACAGTAA	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TCCCTTCTACAAAAGCTCGAAAGGATAACATACTAAGAGAACACAACAGA	1	0.3952569169960474	No Hit
AGGTCTATAGCTCAGATAGGAAGATCACAAGTCTGAACTACAGACAAAAT	1	0.3952569169960474	No Hit
GCAGATTTTTGTGAAGACTCTTACTGGGAAGACCATTACTTTGGAGGTAG	1	0.3952569169960474	No Hit
TTTTTTTTTTGCTATTTCTTTCTTTATTGACTTTTAAAACAACATAAGAG	1	0.3952569169960474	No Hit
GCGGAACTACAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAT	1	0.3952569169960474	TruSeq Adapter, Index 8 (97% over 36bp)
GTTTTTTTTTTTTTTTAAGAAATATAATCTCATCTTATTTTTCATTTTTT	1	0.3952569169960474	No Hit
TACATAACAATAGGTAGTGGGACCAGATCGGAAGAGCACACGTCTGAACT	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTCTCGGAATGCCCCCCATGATCGGAAGGGCACACGTCTGAACTCCAGT	1	0.3952569169960474	Illumina Multiplexing Index Sequencing Primer (96% over 30bp)
TTTTTTTTTTATCCAAAAGTTCATTGCTGCAACATTGAAACGAATACACA	1	0.3952569169960474	No Hit
AAATATTCAAATGAGATCTTTGAAGCACATCTGAAGAGCACACTACTGAA	1	0.3952569169960474	No Hit
AAAAATATTATTAGAAAAAACAAACGATATCAAAAGAACACAACTCAAAA	1	0.3952569169960474	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCTGAACAGCACACGACTGA	1	0.3952569169960474	No Hit
AAAGATGAAAAGGACATTGAAAAGAGAGTCAAAGAGTAGATCGGAAGAGC	1	0.3952569169960474	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATGAGCCTAAGATCAGAAGTCAAA	1	0.3952569169960474	No Hit
CCGATGATTCATGATAACTTGACAAGATCGGAAGAGCACACGTCTGAACT	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTTCAACACGCTTGATTTCATCATTCAGCTCTTGCAATGTAGCTTTTCC	1	0.3952569169960474	No Hit
GTTAATTCCGAAGGGCTTGTCGGTCTTGATCTTCTTGACGCCACTGGCGA	1	0.3952569169960474	No Hit
GCCAAAGAGAAACCTTCCATTGGCACCATAGTAGCAGTTGGGCCTGGGAC	1	0.3952569169960474	No Hit
CACGGTACAGACCCGGGTTCGTTTCCCGGCTAGATCGGAAGAGCACACGT	1	0.3952569169960474	No Hit
AGATTAATAAATGATAGATTAAAATAAAACGTCTGAACACAAGTCACAAA	1	0.3952569169960474	No Hit
GATGAAGAACGAAGCAAGATCGGAAGAGCACAGGTCTGAACTCCAGTCAC	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TTTTTTTTGAAACACGAGACGGGTCTTTTCTTTTATTTAGATAAAAACGT	1	0.3952569169960474	No Hit
AAAACAATATACAAACATAGCAAGAAAAGAAAGAAAGAACAAACGAATGA	1	0.3952569169960474	No Hit
CAAGAAAGCTAGAGCTTGTCAGGCCCGAAGTAGATGGCAAATGTGGGCCT	1	0.3952569169960474	No Hit
GCGACCCCATGAAACTCGGGAGATCAGAAGAGAACACGACAGAACAGAAG	1	0.3952569169960474	No Hit
TTTTTTTTTTTTTTTTTTAAAGAAAGGGAAATAAAAATTAAAAAACATGA	1	0.3952569169960474	No Hit
TCCGAAGTAGAATAGATCGTAAGCATAATCGTCTCAGATCCAAACAGAAA	1	0.3952569169960474	No Hit
GAGAATGATCATGATCGAAGTGATGATGAAGGTTATGGTTCATGTCGGGA	1	0.3952569169960474	No Hit
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAG	1	0.3952569169960474	TruSeq Adapter, Index 7 (97% over 36bp)
GACACGACTCTCGGCAACGGATATCTCGGCAGATCGGAAGGGCACACGTC	1	0.3952569169960474	No Hit
GCGACCCCAGGTCAGTCGGAGATCGCAAGAGCGCACGTCTGAACTCCAGA	1	0.3952569169960474	No Hit
TCCGTTCTAGACTTCGAGCACAAGATAGGAACCGAACACCACACCACTCA	1	0.3952569169960474	No Hit
GCTGATCTACATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTC	1	0.3952569169960474	TruSeq Adapter, Index 1 (97% over 36bp)
GATCTGGTCAGTTAAATCTGGAAGATCGGAAGAGCACACGTCTGAACTCC	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTGAGATCGGAAGAGCAAACGTCTGAACTCCAGTCACGTGCCAATCTCG	1	0.3952569169960474	TruSeq Adapter, Index 19 (97% over 36bp)
TTTTTTTTTTCGAAAGTAAAAGTTGGAATTTTATATGCGTTCAAACACGA	1	0.3952569169960474	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGGATAGCACAAGTCTTG	1	0.3952569169960474	No Hit
TTTTTTTTTTTTTTTTTTTTTGAAAAGCCCCGGAGTTGTTGTTTAAAATG	1	0.3952569169960474	No Hit
AATTTAACCCCCTAATTTTGGATCAAATCGGAAGAGCACACGTCTGAACT	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ATTATAAAAACATAATAACATCACAACTCAAAACAACACTAAAAAACTAA	1	0.3952569169960474	No Hit
TCATCAAACTCTAGATCTTAGATCAGATCGGAAGAGCACACGTCTGAACT	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCATGCTTGATACAGAAAATACACCAAAGCTAGTCGCAAAGAGAGAACT	1	0.3952569169960474	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTAACAAAAAAAAAAAAAAAATAAAAA	1	0.3952569169960474	No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTTTTTTATAAAACTCTGCAAACCAATCTTTGTAAATGGAGAAATAA	1	0.3952569169960474	No Hit
TTTTTTTTTTGCCAACGGATGTGTTTCCTACCGAAGAATTTACATTATTC	1	0.3952569169960474	No Hit
TTTTTTTTTTCCTAGGAATAGATTTTGTTGCCAGTTTTGTCTGTAATCAA	1	0.3952569169960474	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTATGAAAAAAATAAAAATTTAATT	1	0.3952569169960474	No Hit
TTTTTTTTTTGATTTGAACATTTTTACTGGATGTAAAATCCTTCAGTTTT	1	0.3952569169960474	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATGAGATCGGAAGAGCACACGTCTGA	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTTTTTTCCCGAATAAAAGGCCTCTCCGTTTTTGTCTGCCTCTAGTG	1	0.3952569169960474	No Hit
GCGTTTGTAGTCCAACGGTTAGGATAATTGCCTAGATCGGAAGAGCACAC	1	0.3952569169960474	No Hit
GGGGATGTAGCTCAGATCGGAAGGGCACACGTCTGAACTCCAGTCACAAT	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TCGGCTCGGCTCGGATCGGCAGATCGGAAGAGCACACGTCTGAACTCCAG	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATACAACTAATATTAATAAAAAAACGAAAAACAACACTAATAAAAACAA	1	0.3952569169960474	No Hit
CGACACGACTCTCGGCAACGGATATCTCGGCTGACGCAAGATCGGAAGAG	1	0.3952569169960474	No Hit
ACTGATTACTGAATAAATACCAAGATCGGAAGGGCACACGTCTGAACTCC	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTCAACTCCAGTCACAAT	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GCAGGAGGCAAAACATATTAAAGATGAAATCATACAAGTATAAATATGGT	1	0.3952569169960474	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAGAAAAAAGGGGTTGTT	1	0.3952569169960474	No Hit
AACGATGATGAAAAGCTCCGTGACGCAAGATCGGAAGAGCACACGTCTGA	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACACTGTAACTCTGTAAGTAGATCAGAACAGAAGAGCACACGACTGAACT	1	0.3952569169960474	No Hit
CTCCATCAAACTCAAAAAACAAGATCACAAGACAACACTACAGAAAACAA	1	0.3952569169960474	No Hit
AAAAAGCTCGTAGTTGGACTAGATCGGAAGAGCACACGTCTGAACTCCAG	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCGTTGTAGTCTAGCTGGTAAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGACCCCAGGTCAGTCGGGACTAGATCGGAAGAGCACACGTCTGAACTC	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATTTGTACACGTACTTTTCCTTTGGTGGTGGTGGAGAGTGGTAAACCGG	1	0.3952569169960474	No Hit
TCCGTTGTAGTCTAGATGGTCAGGATAAGATAGGAAGAGCACACGTCTGA	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAGAGCGGAAGAGCACACGTCTGA	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GCTAAAGCTCATTTGTCGAATCCTTTTTTCTTTACAACCTGTCTAAAAAC	1	0.3952569169960474	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTAAAACAATTGGAGAGGGTAAAAA	1	0.3952569169960474	No Hit
CGACACCACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACT	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTTTTTTTTGATTGAAACTTGTTTATTACATTAACATTGCCATTACCAT	1	0.3952569169960474	No Hit
GTACGCAAGGGTTCCGATCTTGTTCATGTGCGCAAGGTTCTTGGGTCGCA	1	0.3952569169960474	No Hit
CGAGCACCTCTGAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAATTTTTTTTTTTTATT	1	0.3952569169960474	No Hit
CCGCTGTCGGAGTTGCCGTAGGGATCTGCGGGATGCAGCTGGTCCGCAAT	1	0.3952569169960474	No Hit
ATTTTGCTCTAGATTAGCCACGGGCCAGATCGGAAGAGCACACGTCTGAA	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTTTTTTGGGAAAAAAAAAAGATATAAAAAAAAAAAAAAAAAAAAAA	1	0.3952569169960474	No Hit
TTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.3952569169960474	No Hit
CAGGTGCTCGAATCATCACCAGCAAGATCGGAAGAGCACACGTCTGAATT	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TCCTGGGAAGTCCTCGTGTTGCATTCCAGATCGGAAGAGCACACGTCTGA	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCTACATCGGAAGAGCA	1	0.3952569169960474	No Hit
AAAGCTCGTTGATGGACCTTCGGCCGGGACGGCAGGTGCGCCTCAAGAAC	1	0.3952569169960474	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTAAATATTTGGGAATTTTTTTTTTC	1	0.3952569169960474	No Hit
CTTAGATGTCTATATTTTGTAGCTAAACTAGAATTGGATAGAGAGGAAAA	1	0.3952569169960474	No Hit
TACATGGAAGACATCCGAAGACCACAAATCTGAACACCACTCACAATCGA	1	0.3952569169960474	No Hit
ACACAAAACACTAGTAAAACTATATCTAATAACTCCAAAAAGAAAGAAAA	1	0.3952569169960474	No Hit
AGTGTTTGGATGAGAATAACGTAGAAGATCGGAAGAGCACACGTCTGAAC	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATCCACAAGTGTTCGAATATAACCCCACTAAAAGCGAACGAGATATAG	1	0.3952569169960474	No Hit
TATAATTAACAGGAAACGCTGAAGATCGGAAGAGCACACGTCTGAACTCC	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAAGGGCACCAAACA	1	0.3952569169960474	No Hit
AACGGCTAAGATCTGAGGAAAGCGAGATCGGAAGGGCACACGTCTGAACT	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ATACTGTAGGGGAGGTCCTGCGGCAAAATAGCTCGATGCCAGAATAGATC	1	0.3952569169960474	No Hit
CACCGTCCCGTAGATCCGGGGAGCAGATCGGAAGAGCACACGTCTGAACT	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAGGTAGGAAGAGCCCACGTAAGAA	1	0.3952569169960474	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCAGATCGGAAGAGAACAC	1	0.3952569169960474	No Hit
CTACCGTGTGCCGGATTATGACTGAGATCGGAAGAGCACACGACTGAACA	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
GTGAATCTATCTCATAAAAAAAGAAAAAACGTAAGAACACAAGTCACAAA	1	0.3952569169960474	No Hit
TCTGCCTGGGCGTCACGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCCAATGCTGATGTAGCTAAAGAAATCTTCAAAACCCAAGAACTGAACT	1	0.3952569169960474	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAATCGGCTCTAAGACGGAATCTCA	1	0.3952569169960474	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1	0.3952569169960474	No Hit
TTTTTTTTTTACAAAATCATATTCTACTTCGAAATCAATGTTTGGTATAC	1	0.3952569169960474	No Hit
GGGGATGTAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATA	1	0.3952569169960474	TruSeq Adapter, Index 3 (97% over 36bp)
TTAAAGGGAGAACTGCGAGGAGCCAACCCTAGTACGAGAGGACTAGATCG	1	0.3952569169960474	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGTAGAGCAGACGTCTGA	1	0.3952569169960474	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAAATAGGGCTATAGAACGCTAGAGCA	1	0.3952569169960474	No Hit
AAATCAATAGAACTAATAAATATCAGAACAGAAGTGCACACGACTCAACT	1	0.3952569169960474	No Hit
AATAATTCGAAAAAATATCTTAAAAAAAAACATAATAACAAAAGAAACAA	1	0.3952569169960474	No Hit
ACCGTAGAACTCTATAAGCAAACCATAACATCGGAAAAGCACACCACAGA	1	0.3952569169960474	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGGT	1	0.3952569169960474	No Hit
ATAGATTTGCATCACCAGGAGGGGCATTGGAAAAATGGACGTTGTTTAAG	1	0.3952569169960474	No Hit
TAACCGTAGTAATAGATCGGAAGAGAACACGACTGAACTCCAGTCACATT	1	0.3952569169960474	No Hit
TCAGGGCATGATAGACCAAGTCTATAATGGGAATCTCTCTGATGCTGAGA	1	0.3952569169960474	No Hit
CAAGGACACCAGAATTTTTACTAGATCGGAAGAGCACACGTCTGAACTCC	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTGTTTGTATCGATGTGGGTCAACACGATTTGTCGACATGCTGTCATTG	1	0.3952569169960474	No Hit
GCGCTGCAACGGCCTGCGAGCTAGATCGGAAGAGCACACGTCTGAACTCC	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTTTTTTTTCTGAAAAAGAATGTAATCCGTTCTCCAGTGGATGTATCTG	1	0.3952569169960474	No Hit
TTTTTTTTTTTATTGAAAGATATTTTTTGTAATGTGGTGATAGCTGGTTT	1	0.3952569169960474	No Hit
TTTTTTTTTTGCGTTAAAACATTTTATTTAAAAAGAAGAAACTCTTTTTT	1	0.3952569169960474	No Hit
AGCATGTTCCACAGCTTCCTTTTGTCCAATAACTCCAATTTGGCAAACAC	1	0.3952569169960474	No Hit
AGGACCCCGCTGGCACCTTATGAGAAATCAAAGTCAGATCGGAAGAGCAC	1	0.3952569169960474	No Hit
TCGACCTCTTCAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT	1	0.3952569169960474	TruSeq Adapter, Index 8 (97% over 36bp)
TTTTTTTTTTGAAAACTTCTTCTCTTTAACCATCACATTACATAACAAAG	1	0.3952569169960474	No Hit
CACCGGCACGCCAAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATAGCTATGAGATCTGAGGGATGATCGGAAGAGCACACGTCTGAACTCCA	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
TCCGTTGTAGTCTAGCAGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAAAAAGTATTAAATACTCTGCCAGATCGGAAGAGCACACGTCTGAACT	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCACCAGCGCTGCACTCAATTAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TACTTTTTAGAATTCCTCGTTAGATAATTAGAGAAAACGAAAGAAAGAAA	1	0.3952569169960474	No Hit
TTTTTTTTTTGCTAAATATAGCATTTTATTTCAATATATTCGCGCATTAC	1	0.3952569169960474	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	1	0.3952569169960474	No Hit
GGGGATGTAGCTCAGATCGGAAGGGCACACGTCTGAACTCCAGTCACATA	1	0.3952569169960474	TruSeq Adapter, Index 1 (97% over 35bp)
GTACTAGTAGAGATTTTCGATCGAGATCGGAAGAGCACACGTCTGAACTC	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TACTTTGATGAAAAACTCAATACTAACATCAAGAAAGAAAGAACAAAAGA	1	0.3952569169960474	No Hit
GGAAAACATATGAAATCCGAGTTTAGATATGCTTCATGGCATTTTATTGA	1	0.3952569169960474	No Hit
TTTTTTTTTTTTTTTTTTTTTTTAAAAAAGGTTTTTTTTTGTTTAATAAA	1	0.3952569169960474	No Hit
TTTTTTTTTTCGTCATCAAGAACTTTCTTATTAGAAGGAAAAAAACTTTA	1	0.3952569169960474	No Hit
TCCGTTGTAGTCTAGCTGGTGAGGATATTAGCTCTGAAGAGCACCAGTCT	1	0.3952569169960474	No Hit
ACAAATAATCTCATTAACATTAAGATCGGAAGAGCACACGTCTGAACTCC	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAAGACACTCCCCAACTGATATCTAAGATCTCAATAGAACACAACTCAA	1	0.3952569169960474	No Hit
TAATGCATTGGATGCAAGCCCATGCAGATCGCAAGCGAAAACGAATCAAA	1	0.3952569169960474	No Hit
AGCTCAAATTTTAAATCTGAAGATCTGAACAGCACACGACTGAACACCTG	1	0.3952569169960474	No Hit
TCCGTTGTAGTCTAGATGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTCGGTCACATAGATTGGATTGTAGATCGGAAGAGCACACGTCTGAACT	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGAGATAGGAAGAGCACACGT	1	0.3952569169960474	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAATTT	1	0.3952569169960474	No Hit
AGATCGGAAGAGCACCACGTCTGAACTCCGTCACATACTTATCTCGTATG	1	0.3952569169960474	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCTAGATAGGAAGAGCA	1	0.3952569169960474	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAATCGGCTCAACTACGGAAGCTCA	1	0.3952569169960474	No Hit
TTTTTTTTTTTTTTTTTTTTTAAAAAAATGACAAAAAGAATAAAGTGCGG	1	0.3952569169960474	No Hit
CTTGTTCGTTTCTACCGGATAGGTAGATCGGAAGAGCACAAGTCTGAACT	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TTTTTTTTTTGGCAACAACAACAAAATCGATACAGAAACAAACAAAAAAA	1	0.3952569169960474	No Hit
GTTGGCCTTCGTGAACGGAGTAATGATTAGATCTAAAGCGCACAAGTCTG	1	0.3952569169960474	No Hit
TCACCACAATAAAAGTTATAGAATATACCAAACAAACAAATATGAAAACA	1	0.3952569169960474	No Hit
TCGGTTCAGCCGGAGGTAGGGTCCAGATCGGAAGAGCACACGTCTGAACT	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATGGAGAAAGAGTGTTCAGAGGCGTGTTCGAAAGCGGCGACGCATAGATG	1	0.3952569169960474	No Hit
TACTTGTAGATCTAGCATTTCCAGATCGGAAGAGAACACGTAAGAACACC	1	0.3952569169960474	No Hit
GCCGAACTGTAAATACTCTCGTCCAGATCGGAAGAGCACACGTCTGAACT	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTTTTTTTTTTTTTTTTTTTTTGGGGGAAATTTGATGTATAGTGAGGTA	1	0.3952569169960474	No Hit
TTTTTTTTTTTTTTTTTTTATAAAGAGGATTGAGATGGATGTTTTTTTAA	1	0.3952569169960474	No Hit
GTAAAGTTCAGCATTGACACCAACGTCATAAGTTGGGATGTCACCTTTTC	1	0.3952569169960474	No Hit
TTTTTTTTTTACAAAGTGGTCAGCTCGAGGACGAATATATAATGTTTTGC	1	0.3952569169960474	No Hit
CTCGGATTCGCATGTCGACCCCAGCAAAACGGTGCCGACCAAGAAGAAGG	1	0.3952569169960474	No Hit
CGGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCGTATC	1	0.3952569169960474	TruSeq Adapter, Index 9 (97% over 37bp)
AACGAATAAGAAAATATCATCAACAAACAACGAAACGCAACACTAATCAA	1	0.3952569169960474	No Hit
CACGGGCGCCAAGAACCTCTAAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTTTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	1	0.3952569169960474	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAAGGTCGGAAGAGCACACGTCTGA	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAAAGATCGGAAG	1	0.3952569169960474	No Hit
ATTTTAACGCAAAATCGGAACCAAGCATAAGAACAGAACAGGACTCCACT	1	0.3952569169960474	No Hit
GGGGATGTAGCTCAGATCGAAAGAGCACACGTCTGAACTCCAGTCACAAT	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
ACTTAACATTATCGGTCCATGCTAGATCGGAAGAGCACACGTCTGAACTC	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCTTCGATGTCGGCTCTTCCTAAGATCGGAAGAGCACACGTCTGAACTC	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT	1	0.3952569169960474	TruSeq Adapter, Index 8 (97% over 36bp)
ATTATTTGTCTGCTAACTGTTAGTAGATCGGAAGAGCACACGTCTGAACT	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGCCAGATCGCAAGAGAACAC	1	0.3952569169960474	No Hit
ACGTGCTACTCGGATAACCGTAGTAATTAGATCGGAAGAGCACACGTCTG	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCAAGAGCCGATCTCGACGAATGAAGAACGGAAGAGCACACGACTCAACT	1	0.3952569169960474	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAATAGCCTAAGAAAACGAATCTCA	1	0.3952569169960474	No Hit
AATTTTAATAGGGGACGACGACGAAACACAGGACAATATAAAGAAACATA	1	0.3952569169960474	No Hit
AGAAGCTCACATGGTAGAGCAGATCGGAAGAGCACACGTCAGAACTCCAG	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AATTGTAATTTTGGTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAG	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAAGACAATACATCCAACTAGTCAACAACGCAAAACCACACTAATCAAAA	1	0.3952569169960474	No Hit
TTTTTTTTTAAAATAAACAGAGAAGCATTAGGAAGAAGAGACCACGATAT	1	0.3952569169960474	No Hit
TTTTTTTTTTGACTTATCTAACTCACTCTTTTTCTCTCTTTTATTATTTC	1	0.3952569169960474	No Hit
AGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT	1	0.3952569169960474	TruSeq Adapter, Index 12 (100% over 36bp)
GCGGGGATAGCTCAGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAATCTCAGATCGGAACACCTCACC	1	0.3952569169960474	No Hit
GTGCAATCCGGTCGAGTCGGACCAGATCCGAAGAGCACACGTCTGAACTC	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ACCGACCGGCTCGACCCTAGAACGGAAGAGCACACGTCTGAACTCCAGTC	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GCGGAGGAGAAGAAACTTACGAGGATTCTCCATATCGGAAGATCACACGT	1	0.3952569169960474	No Hit
AAAGCCCAGAGCTCAGAAGATAGTAAGAACGAACCGCACAAGTCAATACA	1	0.3952569169960474	No Hit
GCACCAGTGGTCTAGTGGTAGAATAGTACCCAGATCGGAAGAGCACACGT	1	0.3952569169960474	No Hit
GCTTGATCCAACCGCTACATACGAATAGGAGCCTCTGAACAGAGGACCAC	1	0.3952569169960474	No Hit
AAAAACATGGAAATCTAACAAGAACAAACGACAGAACACCAATGAACACC	1	0.3952569169960474	No Hit
TCTCGTGTGGAACAAAAGGAGATCGGAAGAGCACACGTCTGAACTCCAGT	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGTGGCTGTAGTTTAGTGGTGAGAATTCGACCTCGTAAGACCACAGGGCA	1	0.3952569169960474	No Hit
CAGCTACAGAAAAGTTGCTGCAAGAGACAATAGAAAAGCACCAAGCAGAA	1	0.3952569169960474	No Hit
AGTCGAGCTGGCCACGAGTCGAGCAGATCGGAAGAGCACACGTCTGAACT	1	0.3952569169960474	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.3952569169960474	0.0	0.0	0.0	0.0
2	0.3952569169960474	0.0	0.0	0.0	0.0
3	0.3952569169960474	0.0	0.0	0.0	0.0
4	0.3952569169960474	0.0	0.0	0.0	0.0
5	0.7905138339920948	0.0	0.0	0.0	0.0
6	0.7905138339920948	0.0	0.0	0.0	0.0
7	0.7905138339920948	0.0	0.0	0.0	0.0
8	1.1857707509881423	0.0	0.0	0.0	0.0
9	1.1857707509881423	0.0	0.0	0.0	0.0
10	1.1857707509881423	0.0	0.0	0.0	0.0
11	1.1857707509881423	0.0	0.0	0.0	0.0
12	1.1857707509881423	0.0	0.0	0.0	0.0
13	1.1857707509881423	0.0	0.0	0.0	0.0
14	9.486166007905139	0.0	0.0	0.0	0.0
15	9.486166007905139	0.0	0.0	0.0	0.0
16	9.486166007905139	0.0	0.0	0.0	0.0
17	10.276679841897232	0.0	0.0	0.0	0.0
18	11.067193675889328	0.0	0.0	0.0	0.0
19	11.857707509881424	0.0	0.0	0.0	0.0
20	12.25296442687747	0.0	0.0	0.0	0.0
21	13.83399209486166	0.0	0.0	0.0	0.0
22	14.624505928853756	0.0	0.0	0.0	0.0
23	16.99604743083004	0.0	0.0	0.0	0.0
24	18.972332015810277	0.0	0.0	0.0	0.0
25	25.691699604743082	0.0	0.0	0.0	0.0
26	26.08695652173913	0.0	0.0	0.0	0.0
27	26.48221343873518	0.0	0.0	0.0	0.0
28	36.36363636363637	0.0	0.0	0.0	0.0
29	36.758893280632414	0.0	0.0	0.0	0.0
30	36.758893280632414	0.0	0.0	0.0	0.0
31	36.758893280632414	0.0	0.0	0.0	0.0
32	37.54940711462451	0.0	0.0	0.0	0.0
33	37.54940711462451	0.0	0.0	0.0	0.0
34	38.3399209486166	0.0	0.0	0.0	0.0
35	38.3399209486166	0.0	0.0	0.0	0.0
36	38.73517786561265	0.0	0.0	0.0	0.0
37	38.73517786561265	0.0	0.0	0.0	0.0
38	39.130434782608695	0.0	0.0	0.0	0.0
39	39.52569169960474	0.0	0.0	0.0	0.0
40	39.52569169960474	0.0	0.0	0.0	0.0
41	39.92094861660079	0.0	0.0	0.0	0.0
42	39.92094861660079	0.0	0.0	0.0	0.0
43	39.92094861660079	0.0	0.0	0.0	0.0
44	39.92094861660079	0.0	0.0	0.0	0.0
45	40.316205533596836	0.0	0.0	0.0	0.0
46	40.71146245059288	0.0	0.0	0.0	0.0
47	40.71146245059288	0.0	0.0	0.0	0.0
48	41.10671936758893	0.0	0.0	0.0	0.0
49	41.10671936758893	0.0	0.0	0.0	0.0
50	41.10671936758893	0.0	0.0	0.0	0.0
51	41.10671936758893	0.0	0.0	0.0	0.0
52	41.10671936758893	0.0	0.0	0.0	0.0
53	41.10671936758893	0.0	0.0	0.0	0.0
54	41.10671936758893	0.0	0.0	0.0	0.0
55	41.10671936758893	0.0	0.0	0.0	0.0
56	41.10671936758893	0.0	0.0	0.0	0.0
57	41.10671936758893	0.0	0.0	0.0	0.0
58	41.10671936758893	0.0	0.0	0.0	0.0
59	41.10671936758893	0.0	0.0	0.0	0.0
60	41.10671936758893	0.0	0.0	0.0	0.0
61	41.10671936758893	0.0	0.0	0.0	0.0
62	41.10671936758893	0.0	0.0	0.0	0.0
63	41.10671936758893	0.0	0.0	0.0	0.0
64	41.10671936758893	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE
Rejected 12 READS because READLEN < 1
Read 12 spots for SRR11699706.sra
Written 12 spots for SRR11699706.sra
Rejected 12 READS because READLEN < 1
Read 12 spots for SRR11699706.sra
Written 12 spots for SRR11699706.sra
Rejected 12 READS because READLEN < 1
Read 12 spots for SRR11699706.sra
Written 12 spots for SRR11699706.sra
Rejected 12 READS because READLEN < 1
Read 12 spots for SRR11699706.sra
Written 12 spots for SRR11699706.sra
Rejected 12 READS because READLEN < 1
Read 12 spots for SRR11699706.sra
Written 12 spots for SRR11699706.sra
Rejected 12 READS because READLEN < 1
Read 12 spots for SRR11699706.sra
Written 12 spots for SRR11699706.sra
Rejected 12 READS because READLEN < 1
Read 12 spots for SRR11699706.sra
Written 12 spots for SRR11699706.sra
Rejected 12 READS because READLEN < 1
Read 12 spots for SRR11699706.sra
Written 12 spots for SRR11699706.sra
Rejected 12 READS because READLEN < 1
Read 12 spots for SRR11699706.sra
Written 12 spots for SRR11699706.sra
Rejected 12 READS because READLEN < 1
Read 12 spots for SRR11699706.sra
Written 12 spots for SRR11699706.sra
Rejected 12 READS because READLEN < 1
Rejected 12 READS because READLEN < 1
Read 12 spots for SRR11699706.sra
Read 12 spots for SRR11699706.sra
Written 12 spots for SRR11699706.sra
Written 12 spots for SRR11699706.sra
Rejected 12 READS because READLEN < 1
Read 12 spots for SRR11699706.sra
Written 12 spots for SRR11699706.sra
Rejected 12 READS because READLEN < 1
Read 12 spots for SRR11699706.sra
Written 12 spots for SRR11699706.sra
Rejected 12 READS because READLEN < 1
Read 12 spots for SRR11699706.sra
Written 12 spots for SRR11699706.sra
Rejected 12 READS because READLEN < 1
Read 12 spots for SRR11699706.sra
Written 12 spots for SRR11699706.sra
Rejected 12 READS because READLEN < 1
Rejected 12 READS because READLEN < 1
Read 12 spots for SRR11699706.sra
Read 12 spots for SRR11699706.sra
Written 12 spots for SRR11699706.sra
Written 12 spots for SRR11699706.sra
Rejected 25 READS because READLEN < 1
Rejected 12 READS because READLEN < 1
Read 25 spots for SRR11699706.sra
Read 12 spots for SRR11699706.sra
Written 25 spots for SRR11699706.sra
Written 12 spots for SRR11699706.sra
SRR ids: ['SRR11699706.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_uy0zytxi
SRR11699706.sra spots: 253
blocks: [[1, 12], [13, 24], [25, 36], [37, 48], [49, 60], [61, 72], [73, 84], [85, 96], [97, 108], [109, 120], [121, 132], [133, 144], [145, 156], [157, 168], [169, 180], [181, 192], [193, 204], [205, 216], [217, 228], [229, 253]]
SRR11699706 file size 46
SRR11699706 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR11699706 SRR11699706_1.fastq
Input file:	SRR11699706_1.fastq
trimmed:	SRR11699706-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	20
Sun Jun 22 03:31:06 2025 >> started

Sun Jun 22 03:31:06 2025 >> done (0.002s)
253 reads processed; of these:
 30 (11.86%) short reads filtered out after trimming by size control
  0 ( 0.00%) empty reads filtered out after trimming by size control
223 (88.14%) reads available; of these:
 93 (41.70%) trimmed reads available after processing
130 (58.30%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  3	  1.35%
 19	  3	  1.35%
 20	  4	  1.79%
 21	  3	  1.35%
 22	  7	  3.14%
 23	  6	  2.69%
 24	 20	  8.97%
 25	  1	  0.45%
 26	  3	  1.35%
 27	 29	 13.00%
 28	  1	  0.45%
 29	  0	  0.00%
 30	  1	  0.45%
 31	  3	  1.35%
 32	  0	  0.00%
 33	  1	  0.45%
 34	  0	  0.00%
 35	  1	  0.45%
 36	  2	  0.90%
 37	  1	  0.45%
 38	  1	  0.45%
 39	  1	  0.45%
 40	  1	  0.45%
 41	  0	  0.00%
 42	  0	  0.00%
 43	  0	  0.00%
 44	  1	  0.45%
 45	  0	  0.00%
 46	  0	  0.00%
 47	  0	  0.00%
 48	  0	  0.00%
 49	  0	  0.00%
 50	  0	  0.00%
 51	  0	  0.00%
 52	  0	  0.00%
 53	  0	  0.00%
 54	  0	  0.00%
 55	  0	  0.00%
 56	  0	  0.00%
 57	  0	  0.00%
 58	  0	  0.00%
 59	  0	  0.00%
 60	  0	  0.00%
 61	  0	  0.00%
 62	  0	  0.00%
 63	  0	  0.00%
 64	  0	  0.00%
 65	  0	  0.00%
 66	  0	  0.00%
 67	  0	  0.00%
 68	  0	  0.00%
 69	  0	  0.00%
 70	  0	  0.00%
 71	  0	  0.00%
 72	  0	  0.00%
 73	  0	  0.00%
 74	  0	  0.00%
 75	  0	  0.00%
 76	130	 58.30%
223 reads passed initial QC
                                 Started job on |	Jun 22 03:31:23
                             Started mapping on |	Jun 22 03:31:23
                                    Finished on |	Jun 22 03:31:24
       Mapping speed, Million of reads per hour |	0.80

                          Number of input reads |	223
                      Average input read length |	55
                                    UNIQUE READS:
                   Uniquely mapped reads number |	17
                        Uniquely mapped reads % |	7.62%
                          Average mapped length |	23.94
                       Number of splices: Total |	0
            Number of splices: Annotated (sjdb) |	0
                       Number of splices: GT/AG |	0
                       Number of splices: GC/AG |	0
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	0
                      Mismatch rate per base, % |	1.23%
                         Deletion rate per base |	0.00%
                        Deletion average length |	0.00
                        Insertion rate per base |	0.00%
                       Insertion average length |	0.00
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	30
             % of reads mapped to multiple loci |	13.45%
        Number of reads mapped to too many loci |	39
             % of reads mapped to too many loci |	17.49%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	53.36%
                     % of reads unmapped: other |	8.07%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	176	176	176
N_multimapping	30	30	30
N_noFeature	14	16	15
N_ambiguous	0	0	0
UnstrandedReadsAssigned:3 PositiveStrandReadsAssigned:1 NegativeStrandReadsAssigned:2
Dataset is classified unstranded
MeadianReadLen=76 20thPercentileLength=24 echo kmer=19
SRR11699706 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 72,539
[index] number of k-mers: 56,563,136
[index] number of equivalence classes: 414,827
[quant] running in single-end mode
[quant] will process file 1: SRR11699706-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 223 reads, 80 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 291 rounds

  72540 SRR11699706.ke.tsv
  44303 SRR11699706.se.tsv
 116843 total
==> SRR11699706.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Zm00001eb096110_T001	2067	1968	0	0
Zm00001eb321680_T001	3417	3318	0	0
Zm00001eb323640_T001	3273	3174	0	0
Zm00001eb314140_T001	893	794	0	0
Zm00001eb080260_T001	3529	3430	0	0
Zm00001eb026490_T001	2433	2334	0	0
Zm00001eb026490_T002	2229	2130	0	0
Zm00001eb026490_T003	2220	2121	0	0
Zm00001eb281360_T001	2412	2313	0	0

==> SRR11699706.se.tsv <==
Zm00001eb015280	0
Zm00001eb000610	0
Zm00001eb033210	0
Zm00001eb044610	0
Zm00001eb014360	0
Zm00001eb051320	0
Zm00001eb021860	0
Zm00001eb011690	0
Zm00001eb016840	0
Zm00001eb052060	0
SRR11699706 completed mapping pipeline successfully
