Starting /dee2/code/volunteer_pipeline.sh SRR11699493
    current disk space = 2473143386112
    free memory = 1520451536 
SRR11699493 SRAfilesize
d383f0757cc3b5d6abcc0044d7038481  SRR11699493.sra
SRR11699493.sra file validated
SRR11699493 is single end
SRR11699493 is conventional basespace
SRR11699493 read1 length is 86 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR11699493_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	543
Sequences flagged as poor quality	0
Sequence length	86
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.244935543278086	32.0	32.0	32.0	21.0	32.0
2	30.683241252302025	32.0	32.0	32.0	32.0	32.0
3	30.688766114180478	32.0	32.0	32.0	32.0	32.0
4	30.43462246777164	32.0	32.0	32.0	21.0	32.0
5	30.34254143646409	32.0	32.0	32.0	21.0	32.0
6	32.86187845303867	36.0	36.0	36.0	14.0	36.0
7	33.721915285451196	36.0	36.0	36.0	21.0	36.0
8	33.28729281767956	36.0	36.0	36.0	21.0	36.0
9	33.32596685082873	36.0	36.0	36.0	21.0	36.0
10-11	33.26979742173113	36.0	36.0	36.0	17.5	36.0
12-13	33.17679558011049	36.0	36.0	36.0	17.5	36.0
14-15	33.19613259668508	36.0	36.0	36.0	21.0	36.0
16-17	32.892265193370164	36.0	36.0	36.0	17.5	36.0
18-19	33.467771639042354	36.0	36.0	36.0	21.0	36.0
20-21	32.61786372007367	36.0	36.0	36.0	14.0	36.0
22-23	32.75598526703499	36.0	36.0	36.0	14.0	36.0
24-25	32.8572744014733	36.0	36.0	36.0	14.0	36.0
26-27	31.933701657458563	36.0	32.0	36.0	14.0	36.0
28-29	29.26427255985267	36.0	17.5	36.0	14.0	36.0
30-31	29.236648250460405	36.0	14.0	36.0	14.0	36.0
32-33	28.935543278084715	36.0	14.0	36.0	14.0	36.0
34-35	29.311233885819522	36.0	14.0	36.0	14.0	36.0
36-37	29.380294659300183	36.0	14.0	36.0	14.0	36.0
38-39	29.15561694290976	36.0	14.0	36.0	14.0	36.0
40-41	29.31767955801105	36.0	17.5	36.0	14.0	36.0
42-43	28.547882136279927	36.0	14.0	36.0	14.0	36.0
44-45	28.345303867403317	36.0	14.0	36.0	14.0	36.0
46-47	28.614180478821364	36.0	14.0	36.0	14.0	36.0
48-49	27.570902394106813	36.0	14.0	36.0	14.0	36.0
50-51	28.03406998158379	36.0	14.0	36.0	14.0	36.0
52-53	27.089318600368323	36.0	14.0	36.0	14.0	36.0
54-55	28.44198895027624	36.0	14.0	36.0	14.0	36.0
56-57	26.975138121546962	32.0	14.0	36.0	14.0	36.0
58-59	27.452117863720076	34.0	14.0	36.0	14.0	36.0
60-61	27.700736648250462	36.0	14.0	36.0	14.0	36.0
62-63	25.37845303867403	29.5	14.0	36.0	14.0	36.0
64-65	25.627071823204417	29.5	14.0	36.0	14.0	36.0
66-67	25.674033149171272	32.0	14.0	36.0	14.0	36.0
68-69	26.17587476979742	32.0	14.0	36.0	14.0	36.0
70-71	26.33977900552486	32.0	14.0	36.0	14.0	36.0
72-73	26.375690607734807	32.0	14.0	36.0	14.0	36.0
74-75	26.413443830570902	32.0	14.0	36.0	14.0	36.0
76-77	26.35451197053407	32.0	14.0	36.0	14.0	36.0
78-79	26.16206261510129	32.0	14.0	36.0	14.0	36.0
80-81	25.903314917127073	32.0	14.0	36.0	14.0	36.0
82-83	26.597605893186007	32.0	14.0	36.0	14.0	36.0
84-85	26.455801104972373	32.0	14.0	36.0	14.0	36.0
86	25.0902394106814	27.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	12.0
21	15.0
22	43.0
23	56.0
24	46.0
25	22.0
26	23.0
27	20.0
28	16.0
29	10.0
30	27.0
31	31.0
32	34.0
33	86.0
34	79.0
35	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.60036832412523	16.574585635359114	44.935543278084715	19.88950276243094
2	20.257826887661142	13.812154696132598	12.338858195211786	53.591160220994475
3	21.915285451197054	16.206261510128915	8.287292817679557	53.591160220994475
4	59.11602209944752	19.152854511970535	9.208103130755065	12.52302025782689
5	14.180478821362799	27.624309392265197	45.11970534069982	13.075506445672191
6	12.52302025782689	17.495395948434624	55.61694290976059	14.3646408839779
7	57.0902394106814	13.259668508287293	15.101289134438305	14.548802946593002
8	8.655616942909761	16.022099447513813	60.40515653775322	14.917127071823206
9	11.233885819521179	60.40515653775322	13.259668508287293	15.101289134438305
10-11	37.01657458563536	16.206261510128915	30.847145488029465	15.930018416206263
12-13	13.627992633517497	14.64088397790055	35.45119705340699	36.279926335174956
14-15	36.64825046040516	40.055248618784525	11.141804788213628	12.154696132596685
16-17	14.732965009208105	19.981583793738487	36.187845303867405	29.097605893186003
18-19	56.44567219152854	15.65377532228361	12.98342541436464	14.917127071823206
20-21	17.67955801104972	22.65193370165746	31.123388581952117	28.5451197053407
22-23	38.67403314917127	40.79189686924494	9.576427255985267	10.957642725598527
24-25	35.576036866359445	42.3963133640553	9.12442396313364	12.903225806451612
26-27	15.930018416206263	40.147329650092075	28.360957642725598	15.561694290976058
28-29	27.900552486187845	32.50460405156538	18.69244935543278	20.902394106813997
30-31	25.96685082872928	33.24125230202578	22.099447513812155	18.69244935543278
32-33	32.50460405156538	25.32228360957643	11.141804788213628	31.031307550644566
34-35	32.78084714548803	36.46408839779006	15.930018416206263	14.825046040515655
36-37	33.70165745856354	39.41068139963168	9.023941068139964	17.863720073664823
38-39	28.82136279926335	36.095764272559855	12.246777163904236	22.83609576427256
40-41	16.666666666666664	35.819521178637196	9.208103130755065	38.30570902394107
42-43	21.639042357274402	34.06998158379374	13.075506445672191	31.215469613259668
44-45	25.59852670349908	20.718232044198896	17.495395948434624	36.187845303867405
46-47	16.206261510128915	27.071823204419886	21.54696132596685	35.17495395948435
48-49	24.49355432780847	26.611418047882136	25.59852670349908	23.296500920810313
50-51	13.351749539594843	43.73848987108656	15.930018416206263	26.979742173112335
52-53	16.298342541436465	24.12523020257827	25.78268876611418	33.79373848987109
54-55	13.167587476979742	25.59852670349908	16.85082872928177	44.38305709023941
56-57	18.60036832412523	35.819521178637196	20.073664825046038	25.50644567219153
58-59	13.996316758747698	26.703499079189687	23.572744014732965	35.727440147329645
60-61	11.694290976058932	36.279926335174956	19.337016574585636	32.68876611418048
62-63	15.377532228360957	39.77900552486188	25.23020257826888	19.613259668508288
64-65	12.61510128913444	29.18968692449355	22.92817679558011	35.2670349907919
66-67	20.349907918968693	18.23204419889503	34.714548802946595	26.703499079189687
68-69	11.049723756906078	27.163904235727436	33.33333333333333	28.45303867403315
70-71	15.101289134438305	17.587476979742174	35.45119705340699	31.860036832412526
72-73	28.360957642725598	13.627992633517497	25.69060773480663	32.32044198895028
74-75	13.627992633517497	26.795580110497237	33.33333333333333	26.243093922651934
76-77	26.979742173112335	13.167587476979742	39.134438305709025	20.718232044198896
78-79	12.79926335174954	20.902394106813997	21.731123388581953	44.56721915285451
80-81	24.769797421731123	21.086556169429098	34.622467771639045	19.521178637200737
82-83	14.917127071823206	24.677716390423573	31.215469613259668	29.18968692449355
84-85	15.009208103130756	27.255985267034994	30.570902394106813	27.163904235727436
86	14.391143911439114	28.044280442804425	41.14391143911439	16.420664206642066
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	0.5
21	0.0
22	0.5
23	1.5
24	1.5
25	1.0
26	1.0
27	2.5
28	4.0
29	4.0
30	2.5
31	3.0
32	7.5
33	7.5
34	2.5
35	3.5
36	7.0
37	5.5
38	3.0
39	6.0
40	10.5
41	13.0
42	14.5
43	14.0
44	16.0
45	21.5
46	43.5
47	59.0
48	69.0
49	63.0
50	44.0
51	34.0
52	21.0
53	17.0
54	19.0
55	17.0
56	11.5
57	11.0
58	8.5
59	6.0
60	6.0
61	5.5
62	4.0
63	4.5
64	6.0
65	4.5
66	2.5
67	1.5
68	0.5
69	2.0
70	2.0
71	0.0
72	0.0
73	0.5
74	1.0
75	2.0
76	2.5
77	1.5
78	1.0
79	1.0
80	0.5
81	0.0
82	0.5
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.09208103130755065
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.1841620626151013
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	543.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.87292817679558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.88888888888889	80.29465930018415
2	1.3333333333333335	2.209944751381215
3	0.8888888888888888	2.209944751381215
4	0.0	0.0
5	0.2222222222222222	0.9208103130755065
6	0.2222222222222222	1.1049723756906076
7	0.0	0.0
8	0.2222222222222222	1.4732965009208103
9	0.0	0.0
>10	0.0	0.0
>50	0.2222222222222222	11.786372007366483
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	64	11.786372007366483	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAT	8	1.4732965009208103	TruSeq Adapter, Index 8 (97% over 36bp)
TCCGTTGTAGTCTAGGTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	6	1.1049723756906076	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATT	5	0.9208103130755065	TruSeq Adapter, Index 8 (97% over 36bp)
TCCGTTGTAGTCTAGTTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	3	0.5524861878453038	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCC	3	0.5524861878453038	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGTTGTAGTCTAGCTGGTAAGGATAAGATCGGAAGAGCACACGTCTGA	3	0.5524861878453038	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGG	3	0.5524861878453038	TruSeq Adapter, Index 15 (97% over 36bp)
GGCAGAAATCACATCGCGTCAACACCCGCCGCGGGCCTTCGCGATGCTTT	2	0.3683241252302026	No Hit
GCACCAGTGGTCTAGTGGTAGAATAGTACCAGATCGGAAGAGCACACGTC	2	0.3683241252302026	No Hit
TCCGTTGTAGTCTAGATGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	2	0.3683241252302026	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGAACGAACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAG	2	0.3683241252302026	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTA	2	0.3683241252302026	No Hit
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTT	2	0.3683241252302026	TruSeq Adapter, Index 12 (97% over 36bp)
GGGGATGTAGCTCAGATCGGAAGAGCACAAGTCTGAACTCCAGTCACAAT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAGAACGGATGAAGACACCACTGA	1	0.1841620626151013	No Hit
TCAGGCTCGCACGATACTTAAAGCACATCGCAAAAGCACACGACTGAAAT	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAATAAGATAAGAAGAGCACACTTC	1	0.1841620626151013	No Hit
GGGGATGTAGCTCAGATCAGATCGGAAGAGCTCACGACTCAACTCCACTC	1	0.1841620626151013	No Hit
AACAGGTCTTTAGAAATTAATTGAAGATCGGAAGAGAACACAACTGAACT	1	0.1841620626151013	No Hit
CCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGG	1	0.1841620626151013	No Hit
AAGGAAGCTATAAGTAATGAAACTATGAAAGATCGGAAGAGCACAAGTCT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTACGGTTCATCCCG	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGTAAGAGCTCAAGACAGA	1	0.1841620626151013	No Hit
TCAGGAGAGATGACACCGACGAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAG	1	0.1841620626151013	No Hit
ATAAAACCAAAGTAAGACTGTGAGATCGGAAGAGCACACGACTGAACTCC	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATG	1	0.1841620626151013	TruSeq Adapter, Index 15 (100% over 36bp)
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTAGCTCGGGAGAGCACGAGTCT	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACAACTCTGA	1	0.1841620626151013	No Hit
TAAAACATCCAAAGCTAACGAAGTGCGCAAGGACGAGATGGGAAAAGCAC	1	0.1841620626151013	No Hit
ACAATCCACTAAAACAAACTCATTAGATCGGAAGAGCACACGTCTGAACT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACACGACTCTCGGCAACGGGTAAGATGGGAAGAGCACACGTCTGAACT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCAC	1	0.1841620626151013	No Hit
AACTATGCCTGAGCGAAATCGGAAGAGCACACGTCTGAACACCAGTCACA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
AGATAAAAGGCTGACGCGGGCTCTAGATCGGAAGAGCACACGTCTGAACT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGCACGGATTCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG	1	0.1841620626151013	TruSeq Adapter, Index 2 (97% over 35bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTAGGATAGATCGGAAGAGCACA	1	0.1841620626151013	No Hit
GGGGATGTAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATG	1	0.1841620626151013	TruSeq Adapter, Index 15 (100% over 36bp)
AGGAAAGAAGCTCAGAACCGAATATCAGAACTCAAAACACCACTAAAAAA	1	0.1841620626151013	No Hit
GGGGATGTAGCTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGG	1	0.1841620626151013	TruSeq Adapter, Index 15 (97% over 36bp)
CTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGT	1	0.1841620626151013	No Hit
AACATTTATGAAACATCTAGATCCGAATAGCAAACGAAAGAAAAACAAAA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGATGGTCAGGATAATCTCGGATAAGAACAAATCTCA	1	0.1841620626151013	No Hit
AGAAGTGGACCGACACGTAGGGGGAGATCGGAAGAGCACACGTCTGAACT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAGCTCCGATGAGCACCAGTCGGA	1	0.1841620626151013	No Hit
GTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATG	1	0.1841620626151013	No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA	1	0.1841620626151013	No Hit
ATCAGAGGATGAAAGCTTGAAAAGATCGGAAGAGCACACGTCTGAACTCC	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGTAAAAGCTCGTTTGATTCTGATTTCCAGTAGATCGGAAGAGCACACG	1	0.1841620626151013	No Hit
CTGACGTGCAAATCGTTCGTCTGACTTGGGTATAGGGGCGAAGATCGGAA	1	0.1841620626151013	No Hit
CGACACGACTCTCGGCAACGGGTTGGGCAGAGCGGAAGAGGGCACGGCTG	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATAGAGCGGAAGCGCACAGGTCAG	1	0.1841620626151013	No Hit
AAAGGGACGAGATCGGAGGAGCACACGTCTGAACTCCAGTCACCGGCGTA	1	0.1841620626151013	TruSeq Adapter, Index 2 (97% over 35bp)
TCCGTTGTAGTCTAGCTGGTCAGGATACAACGGAAGAGGACACGTCAGAA	1	0.1841620626151013	No Hit
CCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTAC	1	0.1841620626151013	No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATGGGAAGAGGAAGCGACAGC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGTTGGTCAGGATGAGATCGGAAGAGCACAAGTCTGA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCCGTTGTAGTCTAGCTGGTCAGGACAGATCGGAAGAGCACACGTCTGAA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGCATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTT	1	0.1841620626151013	TruSeq Adapter, Index 12 (97% over 36bp)
CAAAAGAACTACAGCGGAGCAGCGCACGGATGAGCACAAGTCACCACGCC	1	0.1841620626151013	No Hit
AGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGG	1	0.1841620626151013	No Hit
AGGGAAATATCAAAAATACTATGAAAACACATCTGAACACCACACAAATG	1	0.1841620626151013	No Hit
ATATAGTATATTTAATAGAATGTGAGAGACGAAGATCAGAGGTCTAAACA	1	0.1841620626151013	No Hit
GGGGATGTAGCTAAGATCTGAAGAGGACACGACTGAACTCCAGACACAAA	1	0.1841620626151013	No Hit
CAGACGACTTAAAACGCGACGGGGCATTGTAAGATCGGAAGAGCACACGT	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTGAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTTGTAGTCTAGCTGGTAAGGATATTTTGCTCAGATCTCAAGAGAAC	1	0.1841620626151013	No Hit
CTAACGAACGAACGATTTGAAAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGACACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAACTGTCTTTGGTAATCTGGAAGATCGGAAGAGCACACGTCTGAACTC	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATACGGCAGATCGGAAGAGCACAC	1	0.1841620626151013	No Hit
GCGTCTGTAGTCCAACGGTTAGGATAAGATCGTAAGAGCAGCAGTCTGAC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGGTGGTCAGGATATGATCGGATGAGGAGACGTCTCA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTGAGGATAATATGCTATGGGGAGGAGTCTCA	1	0.1841620626151013	No Hit
CCGGGCGGAGCGGCCGTCGGTGCAGATCTTGGTGGTAGGAGATCGGAAGA	1	0.1841620626151013	No Hit
TCCGATCTACTCTAGCTCGACACGATAAGATCGGAAGAGCTCACGTCTGA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCCAAACAACTATATAATAACCGAAAATAACACCACAAAACTACATACAA	1	0.1841620626151013	No Hit
AAGATGAAAAGGACTTTGAAAAGAGATCGGAAGAGCACACGTCTGAACTC	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAATCTGGGAAGAGGACACCTCTGA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTAAGGATATTATGCGATAGGGAGGACTCTCA	1	0.1841620626151013	No Hit
ACCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATGATCGGAAGAGCAGCCGTCTGC	1	0.1841620626151013	No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG	1	0.1841620626151013	No Hit
AAAACAAAAAAGCTATTTAGACTGAGATAAGAAGAGCACACTTCTCAACT	1	0.1841620626151013	No Hit
GGAGAATTACGGGTCCAAACCCGAGATCCAAAGAGCAACCGACGACTGAA	1	0.1841620626151013	No Hit
TCCGAAGATTCATGAAAACTTAACAGAACCGAAGAGAAAACATAAGAAAT	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAGATACGAGGTCGACACGTCTCAACT	1	0.1841620626151013	No Hit
ATTCGCTGGAGAGCTTTTAAGACGAGATCGGAAGAGCACACGTCTGAACT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGGAGGTAGGGTCCAGCGGCCGGAAGAGCTCGCTTGGTGCAGATCGGAA	1	0.1841620626151013	No Hit
CAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGC	1	0.1841620626151013	No Hit
CCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTGAGCAAGGGAGAGCAGCAGTC	1	0.1841620626151013	No Hit
GGGGATGTAGCTCAGATAGGAAGAGCACACGTCAGAACTACAGTCACAAT	1	0.1841620626151013	No Hit
AGAAATGAAGAAGATTACTTCAAGATCGGAACAGCACACGTCTGGACTCC	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAGGAACGAAGGGGACCCCTAAGGA	1	0.1841620626151013	No Hit
GCCCGCCGATCCGATGATTCATGATAACTTGACGGAGATCGGAAGAGCAC	1	0.1841620626151013	No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA	1	0.1841620626151013	No Hit
CCAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGC	1	0.1841620626151013	No Hit
GGTTTTAAGACATCTCGTACTAAACGAAAGGTAGGAAGAAAACACGTAAG	1	0.1841620626151013	No Hit
AACTTTAGAAAGCACAGACAGAGAGAACGGAACACCACACCTCAGAAAAA	1	0.1841620626151013	No Hit
CTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTT	1	0.1841620626151013	No Hit
ATTTGTAGTTTTTGAATTAGAGTGAGATCGGAAGAGCACACGTCTGAACT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT	1	0.1841620626151013	No Hit
CCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCA	1	0.1841620626151013	No Hit
AATAAAAAAACGTAGGAGAAGCAACTTACATCAGAAGAGAACAAGTCTTA	1	0.1841620626151013	No Hit
AACGATTTAAAACACATCCCAAATAACGAAAAAGCACCGAAATTAACACC	1	0.1841620626151013	No Hit
CACGACTCTCGGCAACGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCTCACGACAGA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAATATCCGAAGCGCAAACGAAAGA	1	0.1841620626151013	No Hit
GAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGCGGGTCTTCCGTAC	1	0.1841620626151013	No Hit
TCCATTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATGTAGGGAGGGCCCCCGGAGTCA	1	0.1841620626151013	No Hit
TGCTCGAGCTGCTCGAGATCGGAGAGCACACGTCGAACTCCAGTCACATA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAAAAGATCGGAAGAGAACAAGTCTGA	1	0.1841620626151013	No Hit
CACTTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAACC	1	0.1841620626151013	No Hit
CTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTT	1	0.1841620626151013	No Hit
GTGGCTGTAGTTTAGTCGTGAGAATTACGCGAGATCGGAAGAGAAAACGT	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGATGGTCAGGATAGATCAGAAGAGCACACGTCAGAA	1	0.1841620626151013	No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGG	1	0.1841620626151013	No Hit
TGGCGCTCTCGCAGACCCGACGCACCCCCGCCACGCAGTTTTATCCGGTA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAATAAGATATGAACAGCACACTTC	1	0.1841620626151013	No Hit
ATAACTGAATTTGTGGTAAAGGGAAGATCAGAAGAGCACACGTCAGAACA	1	0.1841620626151013	No Hit
CCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCG	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGCCTATCAGCCAGATAGGAA	1	0.1841620626151013	No Hit
TACATATTCTTTGTACAAAAACTAATATATGAAGAGAACACGTCTGAACT	1	0.1841620626151013	No Hit
CTGAAACTTAAAGGAATTGACGAGATCGGAAGAGCACACGTCTGAACTCC	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAATAACGGAAGATCACCACTCGGA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGGTGGTCAGGATAAGAAGGGATGAGGAGACCACTGA	1	0.1841620626151013	No Hit
TCCGAAAACCATTATATCCTGAAGATCGGAAGAGCACAAGTCTGAACTCC	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCC	1	0.1841620626151013	No Hit
ACCTATAAGAAACTTCGATATCGGAAGATCAAACGTCTGAACTCCATTAA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAGTAAGGAAGAAAGAACGACAGAC	1	0.1841620626151013	No Hit
CCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCATTTAAAGTTTGAGA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGGTGGTCAGGATAAGATCGTAAGGGGAGGAGTCTGA	1	0.1841620626151013	No Hit
TAGGATAAGTGGGAGCCTCCAGATCGGAAGAGCAAACGTCTGAACTCCAG	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CCGACTCGATCCGACATTATACAAAGATCGGAAGAGTACACGTCTGAACT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATAATTAGTGTCTCCGTAAGAATAGATCGGAAGAGCACACGTCTGAACT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTAGGTTTTGAACTTCTACAAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCT	1	0.1841620626151013	No Hit
TATCAGTCATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCC	1	0.1841620626151013	TruSeq Adapter, Index 18 (97% over 37bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAATATAGAAAGAAAGCGCGAAAGA	1	0.1841620626151013	No Hit
AGCGGGAGCCACCTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACGAAGCCGTCCACGGATCAGCGGGTGTTACTAAGATCGGAAGACCACA	1	0.1841620626151013	No Hit
GGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCGGAC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCTCCTAAGATCGGACGAGAGC	1	0.1841620626151013	No Hit
CCGCCCGTCGCTCCTACCGATTGAAAGATCGGAAGAGCACACGTCTGAAC	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGATGTAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATG	1	0.1841620626151013	TruSeq Adapter, Index 15 (100% over 36bp)
CCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCTAGAACGGAAGAGCA	1	0.1841620626151013	No Hit
TATTAGATAAAAGGCTGACGCGGGCTCTAGATCGGAAGAGCACACGTCTG	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGACCCCAGGTCAGTCGGGACTACCCGCTGAGATCGGAAGAGCACACGT	1	0.1841620626151013	No Hit
CATTTGGTTGTAATGATAGGACGAGAGATCGGAAGAGCACACGTCTGAGC	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ACAAGCATATACACACAGAGATCTCATTATAAAGATACTAAGATCAACAC	1	0.1841620626151013	No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA	1	0.1841620626151013	No Hit
CGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG	1	0.1841620626151013	No Hit
AAAAAGAATATCACAGAAAGATATAGATCGGAACTGCACACGTCTGAACT	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTGAGGATAATCAGATAGGAGGAGAACACGTC	1	0.1841620626151013	No Hit
TGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT	1	0.1841620626151013	No Hit
GTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCC	1	0.1841620626151013	No Hit
CGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTAC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGATGAGGAGACGACTGA	1	0.1841620626151013	No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC	1	0.1841620626151013	No Hit
CTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTC	1	0.1841620626151013	No Hit
AAAAAGCTCTATAATATATTAGATAGATCGGAAGAGCACACGTCTGAACT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCT	1	0.1841620626151013	No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGG	1	0.1841620626151013	No Hit
ACCTTATTTTACTAAATCCTAGGGAGAGAAGCGGTGCAACATCCGAAGAC	1	0.1841620626151013	No Hit
TAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCAGT	1	0.1841620626151013	No Hit
ACAACTGCAATCCGACTGAATAAATAAAAAGAACTGAACAGAACACGACT	1	0.1841620626151013	No Hit
AAAGAACGAAAGATATGAAAACACCAAATATCAACACACTTAACTACTCT	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGATGGTCAGGATAAGGTAGGAAGAGCAAAAGTCTTA	1	0.1841620626151013	No Hit
CGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCGGCGG	1	0.1841620626151013	No Hit
GGGGATGTAGCTCAGATCGTAGAGCGCTCGCTAGGAGATCCGAAGAGCAC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCCAAGATCGGAAGAGCAC	1	0.1841620626151013	No Hit
AAAATAATCAAGTCAAAGAAAAAAATAGGAAACGAAAAAAAAACAAAAAC	1	0.1841620626151013	No Hit
TGGGATGTAGATAAGATCGTAAGAGAACGCTACTGAACTCCAGTCACAGA	1	0.1841620626151013	No Hit
TGCGCCCTCGGCGGACTGGAGAGGCCTCGGGATCCCACCTCGGCCGGCGG	1	0.1841620626151013	No Hit
TGCAAGTATGAACTAATTCGAGATCGGAAGAGCACACGTCTGAACTCCAG	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAACGAACGATTTGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGC	1	0.1841620626151013	No Hit
CTCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATTTAAT	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAGATACGAAAAACACCAGTATTCA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTATCGTAGGAGCACCCGTCTGC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCAAGCTGGTGAGGATGATCGGCTAGAAAGGAAGAGCAAA	1	0.1841620626151013	No Hit
CCGCGGGCCGGTCCCCCCCGCCGGGTCCGCCCCCGGGGCCGCGGTTCCGC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCGGGATAAGATCGGAAGAGCACACGTCTGA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAAAGTTGAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCG	1	0.1841620626151013	No Hit
ACCGAACTACTCTAGCAGGAAAGGATAAGAACCCAACAGCACACCTCTCA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTATCGGATGGGGACGAGTCTCA	1	0.1841620626151013	No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG	1	0.1841620626151013	No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTATCGGAAGAGCAAACGACAGA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTCAGATAGGAAGAGCACACGTC	1	0.1841620626151013	No Hit
GATGACTTGTGGCTAGGGGTGAAAGGACAACCAAGATCGGAAGAGCACAC	1	0.1841620626151013	No Hit
CGGTGCACCAGGGGACTTCACGCAGATCGGAAGAGCACACGTCTGAACTC	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCTAAAAACCCATCTATTTATATAAGAACCTAAAACGACACATCACAACT	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGATAAGAAGGGAAGACGTCTCAACTGAA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGATCAGGATAATCTCGTAGGACCGCACGTCACA	1	0.1841620626151013	No Hit
TTGGTAAGCAGAACTGAGATCGGAAGAGCACACGTCTGAACTCCGTCACA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAAC	1	0.1841620626151013	No Hit
TAAGATTAATACAAGCACTAAAGCATAAAAACGAAAAACAAACCTAATAA	1	0.1841620626151013	No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAATCTCCGAAGAGCACACCTCTGA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGAACTCGTCTGA	1	0.1841620626151013	No Hit
TAAATAGGTAGGACGGCGCGGCAGATCGGAAGAGCACACGTCTGAACTCC	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGTTGTAGTCTAGCTGGTAAGGATATTAGAGGAGGAGCACACCACTGA	1	0.1841620626151013	No Hit
TCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAAGATCGGAAGAGC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGCATAAGAGCGGAAGAGCAAACGAATGA	1	0.1841620626151013	No Hit
GCAAATCGGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAATCGAACCA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATTAGATCGGAAGAGCACACGTCTGA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCAGATCGGAAGAGCACACG	1	0.1841620626151013	No Hit
ATCAGTTATAGTTTGTTTGATGGTAGATCGGAAGAGCACACGTCTGAACT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCAT	1	0.1841620626151013	No Hit
GGGTGTTTGGTCTAGTGGTATGATTAGAAGCGATGAGGAGACCACTGATG	1	0.1841620626151013	No Hit
CTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCATTGAATT	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGATGAGAACACGTCTGA	1	0.1841620626151013	No Hit
GAACAATCGCACAATAATGTCAAGATATGAAGAGCACACTACTCAACTCA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCTTAGTAGCGAACAGC	1	0.1841620626151013	No Hit
ACGGCCCGCCAACCCTGGAATCGGAGATCGGAAGAGCACACGTCTGAACT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGGGCAACACGAATG	1	0.1841620626151013	No Hit
GCGAAACAAGCTCAGAACGGAATACCACAAGACTTAAATCGAAACACAAA	1	0.1841620626151013	No Hit
CCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTG	1	0.1841620626151013	No Hit
AGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTCTCAGGACCG	1	0.1841620626151013	No Hit
CTGTTCTATAGGATCGTACAGATCGGAAGAGCACACGTCTGAACTCCAGG	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAAC	1	0.1841620626151013	No Hit
GTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGGGTCAAATTAAGCCG	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCTCGTAGGATCAGCACTCTGA	1	0.1841620626151013	No Hit
AATCCATACATTAAATATAAACTAAAAAGAGAAAACAACAGAACAAGAGA	1	0.1841620626151013	No Hit
TCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCGCACGTCAGA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAATATCCGATGGGGAGGAGTCTCA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTATCCGATGACCACGCGACGGA	1	0.1841620626151013	No Hit
CGATGTCACGTAAGACTCACTGTGAGATCGGAAGAGCACACGTCTGAACT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATAATGATTAAAAAACAAACAAAAAAGAACGAAAAAGAAAACTAATAAAA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGNATATTAGAAGGGAACACGACACCTCA	1	0.1841620626151013	No Hit
AAAAGGACTTTGAAAAAAGAGATAGGAGGAGCACACGTCTGAACTCCAGT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAGATCGGAAGAGCACACGTCTGAA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCGCGCCGG	1	0.1841620626151013	No Hit
TGGGGATCGGGGATTGCAATTATTCCCCATGAACGAGGAATTCCCAGTAA	1	0.1841620626151013	No Hit
CCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTATATCGCAAGAGCTCA	1	0.1841620626151013	No Hit
GGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTCAGATCGGAAGAGCACACGTC	1	0.1841620626151013	No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT	1	0.1841620626151013	No Hit
CAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAGGTAGGAAGGTCCAACCGGATCA	1	0.1841620626151013	No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTGGGTACGGAGGGGCAC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAATCGGCTATGGGCACGAATCTCA	1	0.1841620626151013	No Hit
GGGGATGTAGCTCAGATGGTAGGAGGACAAGTCTGATCTCCTCACAGCAC	1	0.1841620626151013	No Hit
AGGATGTTGGGGGTACTTACTCTCAGATCGGAAGAGCACACGTCTGAACT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATCACCTATACTAAAATCGGAATAGCACACATATAAACTCAAATCACAC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATATCGGAAGAGCACACGTCTGAA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
ACACGACTATCGGCAAAGGAAATCAAAGCAGAACGGAAAACCAAACTGCA	1	0.1841620626151013	No Hit
ACTGATCGATTGACAAATATCCGAAGAGCACAAGACAACACTACAGAAAA	1	0.1841620626151013	No Hit
CTAACGAACGAACGATTTGAAGAGAGCATGAGAACACGACTCTGCACCCG	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTAAGGATAATCTCGAGAGCGCACGTGTCAGC	1	0.1841620626151013	No Hit
GCGTCTGTAGTCCAAGCGTTAGGATAAGATCGGAAGAGCACACGTCTCAA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AGCCATGCATGTGCAAGTATGAACTAATTCGAATAGATCGGAAGAGCACA	1	0.1841620626151013	No Hit
AACACATTATCAAATAGCTATGTCACTAGAAGATCTGAAAAGATCACAAC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAGATGGTAGGAGGACACCACGTCTCA	1	0.1841620626151013	No Hit
GCGGATGTAGCCAAGTGGATCAAGGCAGTGAGATCGGAAGAGCACACGTC	1	0.1841620626151013	No Hit
AGTAACCGACTCGCAATGAAGATAAGAACAGCACACCACACGACTCCACT	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGGTGGTCAGGATACTCAGATCGGAAGAGCACACGTC	1	0.1841620626151013	No Hit
CAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTA	1	0.1841620626151013	No Hit
CGAAGGCCCGCGGCGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCTCT	1	0.1841620626151013	No Hit
TTATTAGATAAAAGGCTGACGCGGGCTCTGCAGATCGGAAGAGCACACGT	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAATATCGTAGGAGGAGACCTCTTC	1	0.1841620626151013	No Hit
ACACGACTCTCGGCAACGGATATATCGGAACGGAACACCTCACGACTCAA	1	0.1841620626151013	No Hit
CTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCA	1	0.1841620626151013	No Hit
CAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGGCCGTCCCTC	1	0.1841620626151013	No Hit
ACAATTATCCTAAACACATCTTAAGATACCACGAATAAACTAAAATCACA	1	0.1841620626151013	No Hit
GCGACCCCAGGTCAGTCGGGACTACCCGCTGAGTGTAAGCAGATCGGAAG	1	0.1841620626151013	No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTGCAGATCGGAAGAGCA	1	0.1841620626151013	No Hit
GAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG	1	0.1841620626151013	No Hit
TCCGAAATAGTCAAATTGGTAAACAAAATAAACTAAAACCTCAGATCACA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAGAACGGAAGGACACACCGGTGCA	1	0.1841620626151013	No Hit
AAACGAATTATATAGAAGCTGGCTAGATCGGAACAGCACACGTCTGAACT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GCCAAACTCCTAATGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTAA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ACAAGACAAGTAGACCAGCACACAAGATCAGAAGAGCACACGTCTGAACA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
GGTCGTCCGACCTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTA	1	0.1841620626151013	No Hit
GATGAACCGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACC	1	0.1841620626151013	No Hit
GGGGATGTAGCTCAGATCGGAAGAGCACACCTCTGAACAGCAGTCACTAA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGCCATGATCAGAACACCACA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGTAGAGCAAACCTCAGA	1	0.1841620626151013	No Hit
CGAAAGCCGCCGTGGCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCC	1	0.1841620626151013	No Hit
CTCTTGATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC	1	0.1841620626151013	No Hit
TCCGTTGCAGTCTAGGTGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGC	1	0.1841620626151013	No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT	1	0.1841620626151013	No Hit
CCCAGGAATAGTTCACCATCTTTCGGGTCCTAAAACGTGCGCTCGTGCTC	1	0.1841620626151013	No Hit
ACTAACGCTTCAAACTTAACCTGTAGATCGGAAGAGCACACGTCTGAACT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACACTTATAAACTAACTAATAATAAAAATAAAAAAATAGCAAAAGAAAAA	1	0.1841620626151013	No Hit
TGAAGCTGCCAGCATGATCTGAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCGTTGTAGTCTAGATGGTAAGGATAAAGACGGAAAGACAACACTAATG	1	0.1841620626151013	No Hit
GCTCGGGGGTCCCGGCCCCGAAACCGTCCGCAGCTCGGAAGAGCACACGT	1	0.1841620626151013	No Hit
CACGACTCTCGGCAACGGATATCAGATCGGAAGAGCACACGTCTGAACTC	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCCTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCT	1	0.1841620626151013	No Hit
TGGTCGCAAGGCTGAAACTTAAAGGAATTAGATCGGAAGAGCACACGTCT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGACTTTGTGAAATGACTAGATCGGAAGAGCACACGTCTGAACTCCAT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GATTTGAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTA	1	0.1841620626151013	TruSeq Adapter, Index 27 (97% over 39bp)
ATTTTTCGTTGCGTTAGAAACACCAGATCGGAAGAGCACACGTCTGAACT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAATAGATGAAGAGAGCACGGCTGA	1	0.1841620626151013	No Hit
CGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCA	1	0.1841620626151013	No Hit
CACGGCCCCCTGGAGTGCAATGGCGCAATCTTGGCTCACTGCAACCTCCA	1	0.1841620626151013	No Hit
CCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGG	1	0.1841620626151013	No Hit
CACGACTCTCGGCAACGGATATCTAGATCGGAAGAGCACACGTCTGAACT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACACGACTCTCGGCAACGGAAGATCGGTAGAGCGCACGTCTGAACTCC	1	0.1841620626151013	No Hit
CCGACCTTAGCTCAGTTGGTAGAGCGGAGGACTGTAGAAGACCGGAAGAG	1	0.1841620626151013	No Hit
GGGGATGAAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAT	1	0.1841620626151013	TruSeq Adapter, Index 8 (97% over 36bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAGCTCGGAAGAGCACAAGTCTGA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATAAGGCTATCACAGCGGTATGCA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGCTCAGATCGGAAGAGCAC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATAGTAGGAAGGGCACACCTCATG	1	0.1841620626151013	No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGGTTTCGGAATTAACCA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAAGAGCGGAGGAGCACACGAGTCA	1	0.1841620626151013	No Hit
GACAGCAGGACGGTGGTCATGAGATCGGAAGAGCACACGTCTGAACTCCA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATGATCGGAAGAGCACACGTCTGA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CTGTCTATATCAACCAACACATTTTCTGGGGTCTGATGAGCGTCGGCATC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAATATCGGAAGATCACAACACTGA	1	0.1841620626151013	No Hit
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAA	1	0.1841620626151013	TruSeq Adapter, Index 5 (97% over 36bp)
TGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTG	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAGGAGCGGAAGGGAACACGGATCA	1	0.1841620626151013	No Hit
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAG	1	0.1841620626151013	TruSeq Adapter, Index 7 (97% over 36bp)
GCGAAACAAGCTCAAAAGATCGGAACACCACACGTCACAACTCAAATCAC	1	0.1841620626151013	No Hit
TTCAAAAATATATCTCAACTGATAGATCGGAAGATCACAAGTCAGAACTA	1	0.1841620626151013	No Hit
AAAAAATATAATATTCAAGTTAGGAAATATAAAGATCGGAAGAGCACACG	1	0.1841620626151013	No Hit
ATGGGGACGGACTGTAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACAAAAGCAGAACTGGCTAAAGACAGAACAGAACACCACACATCTGAAGT	1	0.1841620626151013	No Hit
AAAGGTTTCCTCGGGCCAGACGGACAAGATCGGAAGAGCACACGTCTGAA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCAGGCGATTGTGGGTTCGGGTCCCACCGGGCGAGCTCGGAAGAGCACAC	1	0.1841620626151013	No Hit
GCGACCCCAGGTCAGTCGGGACTACCCGCTGAGTAGATCGGAAGAGCACA	1	0.1841620626151013	No Hit
GCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTAT	1	0.1841620626151013	No Hit
ACTTCCCCAATATAAATACAAATAAAAACGAAAACCTAACATCACAACTC	1	0.1841620626151013	No Hit
CTAACGAACGAACGATTTGAACAGATCGGAAGAGCACACGAATGAAGACC	1	0.1841620626151013	No Hit
GCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCA	1	0.1841620626151013	No Hit
AGCGGAGTAGAGCAGTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAA	1	0.1841620626151013	No Hit
AGGAATTCATAAAGAAACATGGAAACAACGCAAAAGCAAAACTATGAACA	1	0.1841620626151013	No Hit
CACCCGCCCCGGACACGGACAGGATTGACTGATTGATAGCTCTTTCTCGA	1	0.1841620626151013	No Hit
ACACGACTCTCGGCAACGGATATCTCGGCAGATCGGAAGAGCACACGTCT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGTTGTAGTCTAGCTGGTGAGGATAAGATCGGATGAGCACACGACTGA	1	0.1841620626151013	No Hit
ACACGACTCTCGGCAACGGATATCTAGATCGGAAGAGCACACGTCTGAAC	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACACTACTCTCGGCAACGGATATAAAGATCGGAAAAACACAAGTATG	1	0.1841620626151013	No Hit
CCGTAAAAAGCTAAAAACAAAATAGCAAGAGAAAAAAAAACAAAAACAAT	1	0.1841620626151013	No Hit
GTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAAC	1	0.1841620626151013	No Hit
AATACATAAATAGACCGGCTGAGATATTAAGAGCACACGTCAGAACTCCA	1	0.1841620626151013	No Hit
CTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAG	1	0.1841620626151013	No Hit
CCGAATTAAGAAAAAAGAAGAACAGCACCGAACAGAACAACACTCAACAC	1	0.1841620626151013	No Hit
ACCGGCGCCTCTGCTCGCAGATCGGAAGAGCACACGTCTGAACTCCAGGT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAGTCGGCTCTAGAGAGGAAGCGAA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGGAAGAGCACACGTCAGA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCCGTTGTAGTCTAGCTGGTCAGGATACTAAGGTAAGAGCACACCACGTC	1	0.1841620626151013	No Hit
GGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAGGAACGAAGAGCACACCTGAGAA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGGTGGTCAGGATACTAGCGTAAGAGCGGAAGAGAGC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAGGAACGGAAGGGCACACGACTGA	1	0.1841620626151013	No Hit
CAGCTAAAAAGCTCGTAGTTAGATCGGAAGAGCACACGTCTGAACTCCAG	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACAACCGACTCAGAACTGGTACGGAGATCGGAAGAGCACACGTCTGAAC	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCAT	1	0.1841620626151013	No Hit
CCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACG	1	0.1841620626151013	No Hit
AACGATTTGAACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATC	1	0.1841620626151013	TruSeq Adapter, Index 9 (97% over 37bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAAATCATAGGAGCAGACGTCTGA	1	0.1841620626151013	No Hit
CAGACCCAGATCCTCTCTTAGCAACCCACCATCTTTAGATAGGAAGAGCA	1	0.1841620626151013	No Hit
TTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGC	1	0.1841620626151013	No Hit
GGGGATGTAGCTCAAATCGGAAGAGCACACGTCTGAACTCCAGTCACAAT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
AATCATTAAGAGGAAGGAGAAGTCGTAGATGGGAAGCGCACACGTATGAA	1	0.1841620626151013	No Hit
AAATCGAAAGAACTCCTCTAAATAACATAGGAAGAGCAGACGAATAAACA	1	0.1841620626151013	No Hit
TGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC	1	0.1841620626151013	No Hit
CCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCA	1	0.1841620626151013	No Hit
GCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACC	1	0.1841620626151013	No Hit
CCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCT	1	0.1841620626151013	No Hit
AGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCC	1	0.1841620626151013	No Hit
TCAGAATTCCACGTTGTGGCCGTGGAGAGCTGGAGATCGCAAGAGCACAC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCGCAAGAGCTCACGACTCA	1	0.1841620626151013	No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCCAGATAGGTAGAGCAAACGACT	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTCACGTAAGAACAGAAGTCTGC	1	0.1841620626151013	No Hit
CTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGCGTATGAGCCGACATCGGA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGGTGGTTAGGATACTTAGATCGGAAGAGCACACGTC	1	0.1841620626151013	No Hit
TACCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGG	1	0.1841620626151013	No Hit
TTAAAACCGGGTGTGACAGAAGTGAGATCGGAAGAGCACACGTCTGAACT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGTCATCGCGCTTGGTTGAAAGATCGGAAGAGCACACGTCTGAACTCC	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAGAAGGGAAAAGGAAACGTAATAA	1	0.1841620626151013	No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGGTGGTCAGGATAAGATCGGAAGAGCAGCCGACTGA	1	0.1841620626151013	No Hit
ACGAATCACGCCAACTGAAATATATGCTCTCACAACACATCGGAAGACCA	1	0.1841620626151013	No Hit
AACAGTAAACACTTCGACTCGAACATCTCGTAAGAGCACACGTCTGAACT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATCCCAAGAGCAAGCGAAACA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCAGGTCAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACAAAAGGCAAATAAAATTATAACAACACAAAAACACACAACTCCACA	1	0.1841620626151013	No Hit
GCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGGGCGC	1	0.1841620626151013	No Hit
GGCGAGCCCCGAGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCGCG	1	0.1841620626151013	No Hit
CACACGACTCTCGGCAACGGATATCTCGGCAGATACGAAGAACACACCAC	1	0.1841620626151013	No Hit
GACACGACTCTCGGCAACGGATAAGATCGGAAGAGCACACGTCTGAACTC	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACACGACTCTCGGCAACGGATATCACGGCACTAGCATACGTAAGAAAT	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAATATCGGAAGACCGAACGTCAGA	1	0.1841620626151013	No Hit
AACGGTTCTCTATCTATTTGAAAGATCGGAAGAGCACACGTCTGAACTCC	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTATCGGATGGGAACGAGTCGCA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATACTCACGTAGGAACACAACACTTC	1	0.1841620626151013	No Hit
CACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTG	1	0.1841620626151013	No Hit
TTGGTCATCGCGCTTGGTTGAAAAGATCGGAAGAGCACACGTCTGAACTC	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGA	1	0.1841620626151013	TruSeq Adapter, Index 2 (97% over 36bp)
GCGTCTGTAGTCCAACGGTTAGGATAATATCGTAAGGGAAGAATTATGAA	1	0.1841620626151013	No Hit
ACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCT	1	0.1841620626151013	No Hit
TGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACG	1	0.1841620626151013	No Hit
ACCGTTGTAGTCTAGCTGGTCAGGATAATATGCTATAAAAAGAAGTCTCA	1	0.1841620626151013	No Hit
GCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAA	1	0.1841620626151013	No Hit
ATAAAATGTTATAGACGAGTAAAGACATCTCATGAGGAAAATTCTTAACT	1	0.1841620626151013	No Hit
CATATCGGGTAGGTTGTGGTATTTCATTGCTAGATAGGAAGAGCGCACGT	1	0.1841620626151013	No Hit
ACACGGGGAGGTAGTGACAAGATCGGAAGAGCACACGTCTGAACTCCAGT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT	1	0.1841620626151013	TruSeq Adapter, Index 8 (97% over 36bp)
GTTTCCCTCAGGATAGCTGGCGCTCTCGCAGACCCGACGCACCCCCGCCA	1	0.1841620626151013	No Hit
ATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTG	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAAGATGGGAGAGCACACCTAAGTA	1	0.1841620626151013	No Hit
GGGGATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTT	1	0.1841620626151013	TruSeq Adapter, Index 9 (97% over 36bp)
ATCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAATATCGTAAGAGGAGACGTCGGA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGAGAAGATCGGAAGAGCACACGTCTGA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTGGACTGAAGGGTGCTCCCTAGATCGGAAGAGCACACGTCTGAACTCC	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAG	1	0.1841620626151013	No Hit
CCCCGCCGCTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGC	1	0.1841620626151013	No Hit
AAATCCAAAACTAAAGAGAACGCAAGAGAACTCCACTGAACTCCAAACAG	1	0.1841620626151013	No Hit
ATTAAATATCTAGCAAAATAGTACAACTGAAGAGCACACCACTGAACAAC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAATAAGGGATGAGGAGACCACTTC	1	0.1841620626151013	No Hit
CGGAAACTCCAAAAATATCATGACAGATCGGAAGAGCACACGTCTGAACT	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCC	1	0.1841620626151013	No Hit
GGGGATGTAGCTCAGATCGGAAGAGAACAGCTCTCAACTACAGTCACACT	1	0.1841620626151013	No Hit
AGTAACCTCCAACGAAACGGAAGCAGAACCGAATAACAAACATAAAATAT	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGGCAGGATAAGATCGGAAGAGCACACGTCTGA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGACTGTCCCGTTTTAACCTTAAGAAGAGAAGAAGACAACTCTTCACAA	1	0.1841620626151013	No Hit
TGCCACGATCCACTGAGATCCAGCCCCGCGTCGCACGGATTCGCAGATCG	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATGATCGGAATAGGAAACGGCTGA	1	0.1841620626151013	No Hit
GCGGAGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACA	1	0.1841620626151013	No Hit
GGGCATGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAAA	1	0.1841620626151013	TruSeq Adapter, Index 5 (97% over 36bp)
ATCACAAAAGATGTAGAGAATTTGAACAAAAACCTACATAACTAAATACA	1	0.1841620626151013	No Hit
GGGGATGTAGCTTAGATCGGAAGAGCAAACGACTGAACTCCAGTCACAAT	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATAAGCTCGGAAGAGCGCACGTCTGC	1	0.1841620626151013	No Hit
CAAATGCCTCGTCATCTAATTAGTGAGATCGGAAGAGCACACGTCTGAAC	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCCTCTCCCCC	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGAAGATCGGAAGAGCGCAC	1	0.1841620626151013	No Hit
CGCCGCCCGAATTGTAGTCTGGAAGGTCGGAAGAGCACACGTCTGAACTC	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TCCGTTGTAGTCTAGCTGGTCAGGATAGGATCGGAAGAGCACACGTCTGA	1	0.1841620626151013	Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TCCGTTGTAGGCTAGCTGGTCAGGATAGGTAGGGAGGGCACCCGTATGGA	1	0.1841620626151013	No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATAGATCGGAAGAGAAAACGTATG	1	0.1841620626151013	No Hit
GGGGATGTAGCTCAGATCGGAAGGGCACACGTCTGAACTCCAGTCACAGA	1	0.1841620626151013	TruSeq Adapter, Index 13 (97% over 35bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.1841620626151013	0.0	0.0	0.0	0.0
11	0.1841620626151013	0.0	0.0	0.0	0.0
12	0.1841620626151013	0.0	0.0	0.0	0.0
13	0.5524861878453039	0.0	0.0	0.0	0.0
14	6.813996316758748	0.0	0.0	0.0	0.0
15	6.998158379373849	0.0	0.0	0.0	0.0
16	6.998158379373849	0.0	0.0	0.0	0.0
17	7.366482504604051	0.0	0.0	0.0	0.0
18	8.103130755064457	0.0	0.0	0.0	0.0
19	8.47145488029466	0.0	0.0	0.0	0.0
20	9.023941068139964	0.0	0.0	0.0	0.0
21	10.128913443830571	0.0	0.0	0.0	0.0
22	11.049723756906078	0.0	0.0	0.0	0.0
23	13.259668508287293	0.0	0.0	0.0	0.0
24	14.3646408839779	0.0	0.0	0.0	0.0
25	17.863720073664826	0.0	0.0	0.0	0.0
26	18.784530386740332	0.0	0.0	0.0	0.0
27	19.521178637200737	0.0	0.0	0.0	0.0
28	37.20073664825046	0.0	0.0	0.0	0.0
29	37.569060773480665	0.0	0.0	0.0	0.0
30	38.12154696132597	0.0	0.0	0.0	0.0
31	39.226519337016576	0.0	0.0	0.0	0.0
32	39.96316758747698	0.0	0.0	0.0	0.0
33	40.515653775322285	0.0	0.0	0.0	0.0
34	41.06813996316759	0.0	0.0	0.0	0.0
35	41.62062615101289	0.0	0.0	0.0	0.0
36	42.173112338858196	0.0	0.0	0.0	0.0
37	42.3572744014733	0.0	0.0	0.0	0.0
38	42.5414364640884	0.0	0.0	0.0	0.0
39	42.5414364640884	0.0	0.0	0.0	0.0
40	42.7255985267035	0.0	0.0	0.0	0.0
41	42.9097605893186	0.0	0.0	0.0	0.0
42	43.278084714548804	0.0	0.0	0.0	0.0
43	43.278084714548804	0.0	0.0	0.0	0.0
44	43.278084714548804	0.0	0.0	0.0	0.0
45	43.462246777163905	0.0	0.0	0.0	0.0
46	43.462246777163905	0.0	0.0	0.0	0.0
47	43.462246777163905	0.0	0.0	0.0	0.0
48	43.462246777163905	0.0	0.0	0.0	0.0
49	43.462246777163905	0.0	0.0	0.0	0.0
50	43.462246777163905	0.0	0.0	0.0	0.0
51	43.462246777163905	0.0	0.0	0.0	0.0
52	43.462246777163905	0.0	0.0	0.0	0.0
53	43.462246777163905	0.0	0.0	0.0	0.0
54	43.462246777163905	0.0	0.0	0.0	0.0
55	43.462246777163905	0.0	0.0	0.0	0.0
56	43.462246777163905	0.0	0.0	0.0	0.0
57	43.462246777163905	0.0	0.0	0.0	0.0
58	43.462246777163905	0.0	0.0	0.0	0.0
59	43.462246777163905	0.0	0.0	0.0	0.0
60	43.462246777163905	0.0	0.0	0.0	0.0
61	43.462246777163905	0.0	0.0	0.0	0.0
62	43.462246777163905	0.0	0.0	0.0	0.0
63	43.462246777163905	0.0	0.0	0.0	0.0
64	43.462246777163905	0.0	0.0	0.0	0.0
65	43.462246777163905	0.0	0.0	0.0	0.0
66	43.462246777163905	0.0	0.0	0.0	0.0
67	43.462246777163905	0.0	0.0	0.0	0.0
68	43.462246777163905	0.0	0.0	0.0	0.0
69	43.462246777163905	0.0	0.0	0.0	0.0
70	43.462246777163905	0.0	0.0	0.0	0.0
71	43.462246777163905	0.0	0.0	0.0	0.0
72	43.462246777163905	0.0	0.0	0.0	0.0
73	43.462246777163905	0.0	0.0	0.0	0.0
74	43.462246777163905	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTAGT	20	1.2635892E-5	80.00001	5
CCGTTGT	20	1.2635892E-5	80.00001	2
TAGTCTA	20	1.2635892E-5	80.00001	8
TGTAGTC	20	1.2635892E-5	80.00001	6
TCCGTTG	20	1.2635892E-5	80.00001	1
CGTTGTA	20	1.2635892E-5	80.00001	3
AGTCTAG	20	1.2635892E-5	80.00001	9
GTAGTCT	20	1.2635892E-5	80.00001	7
GTTGTAG	20	1.2635892E-5	80.00001	4
TGGTCAG	20	7.456525E-4	40.000004	16-17
TCTAGCT	20	7.456525E-4	40.000004	10-11
TCAGGAT	20	7.456525E-4	40.000004	20-21
GCTGGTC	20	7.456525E-4	40.000004	14-15
TAGCTGG	20	7.456525E-4	40.000004	12-13
GGTCAGG	20	7.456525E-4	40.000004	18-19
CTGGTCA	20	7.456525E-4	40.000004	16-17
CTAGCTG	20	7.456525E-4	40.000004	12-13
GTCTAGC	20	7.456525E-4	40.000004	10-11
CAGGATA	20	7.456525E-4	40.000004	20-21
GTCAGGA	20	7.456525E-4	40.000004	18-19
>>END_MODULE
Rejected 27 READS because READLEN < 1
Read 27 spots for SRR11699493.sra
Written 27 spots for SRR11699493.sra
Rejected 27 READS because READLEN < 1
Read 27 spots for SRR11699493.sra
Written 27 spots for SRR11699493.sra
Rejected 27 READS because READLEN < 1
Read 27 spots for SRR11699493.sra
Written 27 spots for SRR11699493.sra
Rejected 27 READS because READLEN < 1
Read 27 spots for SRR11699493.sra
Written 27 spots for SRR11699493.sra
Rejected 27 READS because READLEN < 1
Read 27 spots for SRR11699493.sra
Written 27 spots for SRR11699493.sra
Rejected 27 READS because READLEN < 1
Read 27 spots for SRR11699493.sra
Written 27 spots for SRR11699493.sra
Rejected 27 READS because READLEN < 1
Read 27 spots for SRR11699493.sra
Written 27 spots for SRR11699493.sra
Rejected 27 READS because READLEN < 1
Read 27 spots for SRR11699493.sra
Written 27 spots for SRR11699493.sra
Rejected 27 READS because READLEN < 1
Read 27 spots for SRR11699493.sra
Written 27 spots for SRR11699493.sra
Rejected 27 READS because READLEN < 1
Read 27 spots for SRR11699493.sra
Written 27 spots for SRR11699493.sra
Rejected 27 READS because READLEN < 1
Read 27 spots for SRR11699493.sra
Written 27 spots for SRR11699493.sra
Rejected 27 READS because READLEN < 1
Read 27 spots for SRR11699493.sra
Written 27 spots for SRR11699493.sra
Rejected 27 READS because READLEN < 1
Read 27 spots for SRR11699493.sra
Written 27 spots for SRR11699493.sra
Rejected 27 READS because READLEN < 1
Read 27 spots for SRR11699493.sra
Written 27 spots for SRR11699493.sra
Rejected 27 READS because READLEN < 1
Read 27 spots for SRR11699493.sra
Written 27 spots for SRR11699493.sra
Rejected 27 READS because READLEN < 1
Read 27 spots for SRR11699493.sra
Written 27 spots for SRR11699493.sra
Rejected 30 READS because READLEN < 1
Read 30 spots for SRR11699493.sra
Written 30 spots for SRR11699493.sra
Rejected 27 READS because READLEN < 1
Read 27 spots for SRR11699493.sra
Written 27 spots for SRR11699493.sra
Rejected 27 READS because READLEN < 1
Read 27 spots for SRR11699493.sra
Written 27 spots for SRR11699493.sra
Rejected 27 READS because READLEN < 1
Read 27 spots for SRR11699493.sra
Written 27 spots for SRR11699493.sra
SRR ids: ['SRR11699493.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_9lmygsvp
SRR11699493.sra spots: 543
blocks: [[1, 27], [28, 54], [55, 81], [82, 108], [109, 135], [136, 162], [163, 189], [190, 216], [217, 243], [244, 270], [271, 297], [298, 324], [325, 351], [352, 378], [379, 405], [406, 432], [433, 459], [460, 486], [487, 513], [514, 543]]
SRR11699493 file size 110
SRR11699493 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR11699493 SRR11699493_1.fastq
Input file:	SRR11699493_1.fastq
trimmed:	SRR11699493-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	20
Fri Jun 20 09:11:45 2025 >> started

Fri Jun 20 09:11:45 2025 >> done (0.002s)
543 reads processed; of these:
 48 ( 8.84%) short reads filtered out after trimming by size control
  0 ( 0.00%) empty reads filtered out after trimming by size control
495 (91.16%) reads available; of these:
210 (42.42%) trimmed reads available after processing
285 (57.58%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	  2	  0.40%
 19	  4	  0.81%
 20	  6	  1.21%
 21	  5	  1.01%
 22	 13	  2.63%
 23	  8	  1.62%
 24	 22	  4.44%
 25	  5	  1.01%
 26	  6	  1.21%
 27	 98	 19.80%
 28	  1	  0.20%
 29	  3	  0.61%
 30	  6	  1.21%
 31	  5	  1.01%
 32	  3	  0.61%
 33	  5	  1.01%
 34	  4	  0.81%
 35	  4	  0.81%
 36	  3	  0.61%
 37	  1	  0.20%
 38	  1	  0.20%
 39	  1	  0.20%
 40	  1	  0.20%
 41	  2	  0.40%
 42	  0	  0.00%
 43	  0	  0.00%
 44	  1	  0.20%
 45	  0	  0.00%
 46	  0	  0.00%
 47	  0	  0.00%
 48	  0	  0.00%
 49	  0	  0.00%
 50	  0	  0.00%
 51	  0	  0.00%
 52	  0	  0.00%
 53	  0	  0.00%
 54	  0	  0.00%
 55	  0	  0.00%
 56	  0	  0.00%
 57	  0	  0.00%
 58	  0	  0.00%
 59	  0	  0.00%
 60	  0	  0.00%
 61	  0	  0.00%
 62	  0	  0.00%
 63	  0	  0.00%
 64	  0	  0.00%
 65	  0	  0.00%
 66	  0	  0.00%
 67	  0	  0.00%
 68	  0	  0.00%
 69	  0	  0.00%
 70	  0	  0.00%
 71	  0	  0.00%
 72	  0	  0.00%
 73	  0	  0.00%
 74	  0	  0.00%
 75	  0	  0.00%
 76	  0	  0.00%
 77	  0	  0.00%
 78	  0	  0.00%
 79	  0	  0.00%
 80	  0	  0.00%
 81	  0	  0.00%
 82	  0	  0.00%
 83	  0	  0.00%
 84	  0	  0.00%
 85	  0	  0.00%
 86	285	 57.58%
495 reads passed initial QC


criterion=sequence-density
sequence-density=39.19
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=1
prefix-density=0.00
prefix-fanout=1.0
sequence=TCCGTTGTAGTCTAGCTGGTCAGGATA


criterion=fanout-score
sequence-density=39.19
sequence-density-rank=1
fanout-score=0.00
fanout-score-rank=1
prefix-density=0.00
prefix-fanout=1.0
sequence=TCCGTTGTAGTCTAGCTGGTCAGGATA
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	Jun 20 09:11:51
                             Started mapping on |	Jun 20 09:11:51
                                    Finished on |	Jun 20 09:11:52
       Mapping speed, Million of reads per hour |	1.78

                          Number of input reads |	495
                      Average input read length |	56
                                    UNIQUE READS:
                   Uniquely mapped reads number |	27
                        Uniquely mapped reads % |	5.45%
                          Average mapped length |	36.22
                       Number of splices: Total |	0
            Number of splices: Annotated (sjdb) |	0
                       Number of splices: GT/AG |	0
                       Number of splices: GC/AG |	0
                       Number of splices: AT/AC |	0
               Number of splices: Non-canonical |	0
                      Mismatch rate per base, % |	5.73%
                         Deletion rate per base |	0.00%
                        Deletion average length |	0.00
                        Insertion rate per base |	0.00%
                       Insertion average length |	0.00
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	47
             % of reads mapped to multiple loci |	9.49%
        Number of reads mapped to too many loci |	113
             % of reads mapped to too many loci |	22.83%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	52.32%
                     % of reads unmapped: other |	9.90%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	421	421	421
N_multimapping	47	47	47
N_noFeature	27	27	27
N_ambiguous	0	0	0
UnstrandedReadsAssigned:0 PositiveStrandReadsAssigned:0 NegativeStrandReadsAssigned:0
Dataset is classified positive stranded
MeadianReadLen=82 20thPercentileLength=23 echo kmer=19
SRR11699493 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=19

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 19
[index] number of targets: 72,539
[index] number of k-mers: 56,563,136
[index] number of equivalence classes: 414,827
[quant] running in single-end mode
[quant] will process file 1: SRR11699493-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 495 reads, 28 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 100 rounds

  72540 SRR11699493.ke.tsv
  44303 SRR11699493.se.tsv
 116843 total
==> SRR11699493.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Zm00001eb096110_T001	2067	1968	0	0
Zm00001eb321680_T001	3417	3318	0	0
Zm00001eb323640_T001	3273	3174	0	0
Zm00001eb314140_T001	893	794	0	0
Zm00001eb080260_T001	3529	3430	0	0
Zm00001eb026490_T001	2433	2334	0	0
Zm00001eb026490_T002	2229	2130	0	0
Zm00001eb026490_T003	2220	2121	0	0
Zm00001eb281360_T001	2412	2313	0	0

==> SRR11699493.se.tsv <==
Zm00001eb015280	0
Zm00001eb000610	0
Zm00001eb033210	0
Zm00001eb044610	0
Zm00001eb014360	0
Zm00001eb051320	0
Zm00001eb021860	0
Zm00001eb011690	0
Zm00001eb016840	0
Zm00001eb052060	0
SRR11699493 completed mapping pipeline successfully
