Q) My datasets of interest aren't included in DEE. What should I do?
A) Firstly confirm that the species/accession number combination is correct. Then check whether the sequence data is
available from the SRA ftp site. Check that the study raw data is released and not under embargo. If the dataset has been
added recently, then you have a few options. You can run the docker or singularity image on your own server or on the cloud.
The instructions to do this are in the github README.md here.
Alternatively contact us and we'll have it added.
Q) How do I open zip compressed files?
A) Winzip can uncompress .zip files and is available
here for free. Decompression tools are also available from the Apple and Android app store for mobile devices.
Q) What tools are recommended for analyzing RNA-seq count data?
A) There are many options, but we recommend Degust
for new users.
Q) How do I load the data into R?
A) We have written an R function that interfaces with the webpage and loads data into R. For more
information, read the documentation here
Q) Can I load the data directly into my Galaxy history?
A) DEE data can be uploaded manually into Galaxy; we will be investigating direct integration in future.
Q) My DEE data contains multiple technical replicates, how can I aggregate the counts?
A) Counts can be aggregated in R (recommended) as outlined in our guide. Alternatives
include awk or even
spreadsheet software (ie. Excel). If using Excel or other spreadsheet software, please be wary of its default behaviour that converts
gene names and accession numbers to dates and scientific numbers.